CN111965177B - Pesticide residue detection method capable of quickly knowing result - Google Patents
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- CN111965177B CN111965177B CN202010845017.2A CN202010845017A CN111965177B CN 111965177 B CN111965177 B CN 111965177B CN 202010845017 A CN202010845017 A CN 202010845017A CN 111965177 B CN111965177 B CN 111965177B
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Abstract
The invention relates to the technical field of pesticide residue detection, and discloses a pesticide residue detection method capable of quickly knowing a result, wherein a metal catalyst is ground into powder, and the diameter of powder particles is controlled to be 8-10 mu m. According to the pesticide residue detection method capable of quickly knowing the result, a phenolphthalein reagent and an alkaline reagent are colorless liquids, when the test paper soaked with the phenolphthalein solution enters the alkaline reagent, a white test paper clamping piece can be changed into red, when the white test paper clamping piece is changed into red, the white test paper clamping piece is dried, redundant moisture in the test paper is evaporated, and the test paper is restored to a dry state, the test paper clamping piece still keeps a red dominant appearance in the state, the red test paper clamping piece is alkaline, when the red test paper clamping piece is in contact with hydrolysis acid products on stems and leaves, the red test paper clamping piece is changed into colorless, namely the original white state of the test paper, and then after the test paper clamping piece is taken down, the pesticide residue condition in the area can be directly judged through a colorimetric method.
Description
Technical Field
The invention relates to the technical field of pesticide residue detection, in particular to a pesticide residue detection method capable of quickly knowing a result.
Background
The conventional pesticide residue analysis method is an instrumental analysis method, namely, the pesticide content is quickly and accurately analyzed by technologies such as chromatography, chromatography-mass spectrometry, supercritical fluid and the like, but the methods cannot meet the requirement of on-site quick detection of samples, so that people are forced to develop certain pesticide residue quick detection technologies with convenient and quick operation and accurate results, including an enzyme inhibition method, an immunoassay method, a living body bioassay method, a biosensor method and the like.
The existing chemical rapid detection method for pesticide residues sometimes has the defects of unobvious color change, unfavorable judgment and easy interference of plant tissues and chloroplasts in the using process.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a pesticide residue detection method capable of quickly knowing the result, which has the advantages of obvious color comparison, high comparison accuracy and the like, and solves the problems that the existing chemical quick detection method for pesticide residue is not obvious in color change sometimes, is not beneficial to judgment, and is easily interfered by plant tissues and chloroplasts in the use process.
(II) technical scheme
In order to realize the purposes of obvious color contrast and high contrast accuracy, the invention provides the following technical scheme: a pesticide residue detection method capable of quickly knowing a result comprises the following steps:
the method comprises the following steps: grinding the metal catalyst into powder, controlling the diameter of powder particles to be 8-10 mu m, and then putting the powder metal catalyst into purified water according to the proportion of 1: 9;
step two: stirring the mixed liquid obtained in the step one, and quickly filtering the mixed liquid through a filter screen after stirring for 30min, wherein the filtering is to avoid the situation that some larger particles are deposited in the equipment to cause blockage in the spraying process;
step three: the filtered liquid is injected into spraying equipment, and then an experimenter wearing the protective clothing holds a spraying pot filled with catalytic liquid to spray a specified detection area, so that the weight of particles is increased through moisture and the settling speed is accelerated because dust particles are small;
step four: experimenters need to carry out two to three times of circulating spraying in a specified detection area, so that the uniformity of spraying coverage is guaranteed;
step five: before spraying, preparing a plurality of V-shaped card test paper, soaking the test paper clamping pieces in phenolphthalein solution, taking out the test paper clamping pieces, soaking the test paper clamping pieces in alkaline solution, and taking out the test paper after soaking for a period of time;
step six: placing the test paper clamping piece soaked and taken out in the step five into a vacuum drying device for rapid drying, controlling the drying temperature to be 55-60 ℃, taking out the test paper after the moisture on the test paper is completely evaporated, sealing and storing the test paper, and facilitating carrying and use;
step seven: after spraying, clamping the test paper clamping piece on the surface of the plant stem and leaf sprayed with the catalyst, randomly selecting different areas for detection, then taking down the test paper clamping piece, observing the change of the surface color of the test paper, and judging the condition of pesticide residue;
step eight: and after the detection is finished, the plants in the detection area are subjected to spray irrigation cleaning through clear water, and residual reagents and pesticide components on the surfaces of the plants are treated.
Preferably, in the first step, the metal catalyst can be an electrolytic silver catalyst or a molten iron catalyst.
Preferably, in the first step, the smaller the particle size of the dust is, the slower the settling speed is, and the dust with the diameter less than 10 μm is easily inhaled into the human body, whereas the dust with the diameter more than 10 μm can be retained by the upper respiratory tract of the human body, so as to reduce the dust hazard.
Preferably, in the second step, some larger particles still exist in the mixed liquid, so after a period of stirring, large particles in the liquid need to be filtered by a small-gap filter screen, thereby ensuring the passing of the catalyst in the spraying equipment.
Preferably, in the third step, the sprayed liquid contains a large amount of metal ions, while most of the traditional pesticides are organophosphorus pesticides, and the organophosphorus pesticides can be hydrolyzed into alcohol and phosphoric acid under the catalytic action of the metal ions.
Preferably, in the fifth step, the phenolphthalein reagent and the alkaline reagent are colorless liquids, and when the test paper soaked in the phenolphthalein solution enters the alkaline reagent, the white test paper clip turns red.
Preferably, in the sixth step, after the white test paper clip turns red, the white test paper clip is dried, and excess moisture in the test paper is evaporated to return to a dry state, and the test paper clip still keeps a red dominant appearance in the dry state.
Preferably, in the seventh step, the red test paper clip is alkaline, and when the red test paper clip contacts with the hydrolysis acidic product on the stem and leaf, the red test paper clip is changed into colorless, that is, the original white state of the test paper.
Preferably, in the seventh step, after the test paper clamping piece is taken down, the situation of pesticide residues in the area can be directly judged through a colorimetric method.
Preferably, in the step eight, some special soluble pesticides may react chemically when being exposed to alkali, so that the toxicity of the pesticides is enhanced, and therefore, secondary pollution is not avoided, and after the experiment is finished, plants in the area need to be washed.
Compared with the prior art, the invention provides a pesticide residue detection method capable of quickly knowing the result, which has the following beneficial effects:
1. according to the pesticide residue detection method capable of quickly knowing the result, a phenolphthalein reagent and an alkaline reagent are colorless liquids, when test paper soaked with a phenolphthalein solution enters the alkaline reagent, a white test paper clamping piece can be changed into red, when the white test paper clamping piece is changed into red, the white test paper clamping piece is dried, redundant moisture in the test paper is evaporated, and the test paper is restored to a dry state, the test paper clamping piece still keeps a red dominant appearance in the state, the red test paper clamping piece is alkaline, when the red test paper clamping piece is in contact with hydrolysis acid products on stems and leaves, the red test paper clamping piece can be changed into colorless, namely the original white state of the test paper, and then after the test paper clamping piece is taken down, the pesticide residue condition in the area can be directly judged through a colorimetric method.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A pesticide residue detection method capable of quickly knowing a result comprises the following steps:
the method comprises the following steps: grinding the metal catalyst into powder, controlling the diameter of powder particles to be 8-10 mu m, and then putting the powder metal catalyst into purified water according to the proportion of 1: 9;
step two: stirring the mixed liquid in the step one for 30min, and quickly filtering the mixed liquid through a filter screen;
step three: injecting the filtered liquid into spraying equipment, and then spraying the specified detection area by holding a spraying pot filled with catalytic liquid by experimenters wearing protective clothing;
step four: experimenters need to carry out two to three times of circulating spraying in a specified detection area, so that the uniformity degree of spraying coverage is guaranteed;
step five: before spraying, preparing a plurality of V-shaped card test paper, soaking the test paper clamping pieces in phenolphthalein solution, taking out the test paper clamping pieces, soaking the test paper clamping pieces in alkaline solution, and taking out the test paper after soaking for a period of time;
step six: placing the test paper clamping piece soaked and taken out in the step five into a vacuum drying device for rapid drying, controlling the drying temperature to be 55-60 ℃, and taking out the test paper after the moisture on the test paper is completely evaporated, and sealing and storing the test paper;
step seven: after spraying, clamping the test paper clamping piece on the surface of the plant stem and leaf sprayed with the catalyst, randomly selecting different areas for detection, then taking down the test paper clamping piece, observing the change of the surface color of the test paper, and judging the condition of pesticide residue;
step eight: and after the detection is finished, the plants in the detection area are subjected to spray irrigation and cleaning through clear water, and residual reagents and pesticide components on the surfaces of the plants are treated.
Further, in the step one, the metal catalyst can be an electrolytic silver catalyst or a molten iron catalyst.
Further, in the first step, the smaller the particle size of the dust is, the slower the settling speed is, and the dust with the diameter less than 10 μm is easily absorbed into the human body, whereas the dust with the diameter more than 10 μm can be blocked by the upper respiratory tract of the human body, so that the dust harm is reduced.
Further, in the second step, some larger particles still exist in the mixed liquid, so after a period of stirring, large particles in the liquid need to be filtered by a small-gap filter screen, and the passing performance of the catalyst in the spraying equipment is guaranteed.
Furthermore, in the third step, the sprayed liquid contains a large amount of metal ions, while most of the traditional pesticides are organophosphorus pesticides, and the organophosphorus pesticides can be hydrolyzed into alcohol and phosphoric acid under the catalytic action of the metal ions.
Furthermore, in the fifth step, the phenolphthalein reagent and the alkaline reagent are colorless liquids, and when the test paper soaked in the phenolphthalein solution enters the alkaline reagent, the white test paper clamping piece turns red.
Further, in the sixth step, after the white test paper clip is changed into red, the white test paper clip is dried, redundant moisture in the test paper is evaporated, the test paper clip is restored to a dry state, and the test paper clip still keeps a red dominant appearance in the dry state.
Further, in the seventh step, the red test paper clip is alkaline, and when the red test paper clip is contacted with the hydrolysis acid product on the stem leaves, the red test paper clip is changed into colorless test paper, namely the original white state of the test paper.
And further, in the seventh step, after the test paper clamping piece is taken down, the situation of pesticide residue in the area can be directly judged through a colorimetric method.
Furthermore, in step eight, some special soluble pesticides may react chemically when meeting alkali, so that the toxicity of the pesticides is enhanced, secondary pollution is not avoided, and plants in the area need to be washed after the experiment is finished.
When in use, grinding the metal catalyst into powder, controlling the particle diameter of the powder to be 8-10 microns, then putting the powder metal catalyst into purified water according to the proportion of 1:9, stirring the mixed liquid in the first step for 30min, quickly filtering the mixed liquid through a filter screen, injecting the filtered liquid into spraying equipment, then spraying the appointed detection area by an experimenter wearing the protective clothing with a spraying pot holding catalytic liquid, wherein the experimenter needs to spray circularly for two to three times in the appointed detection area to ensure the uniform degree of spraying coverage, before spraying, preparing a plurality of V-shaped card test paper, firstly soaking the test paper clips in a phenolphthalein solution, then taking out the test paper clips, soaking the test paper clips in an alkaline solution for a period of time, then taking out the test paper clips soaked and taken out in the fifth step, placing the test paper clips in a vacuum drying device for quick drying, the drying temperature is controlled to be 55-60 ℃, the test paper is taken out for sealing and storage after the moisture on the test paper is completely evaporated, after spraying is finished, the test paper clamping pieces are clamped on the surfaces of plant stems and leaves sprayed with the catalyst, different areas are randomly selected for detection, then the test paper clamping pieces are taken down, the change of the surface color of the test paper is observed, the condition of pesticide residues is judged, after detection is finished, plants in the detection area are subjected to spray irrigation and cleaning through clear water, and residual reagents and pesticide components on the surfaces of the plants are treated.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A pesticide residue detection method capable of quickly knowing a result is characterized by comprising the following steps:
the method comprises the following steps: grinding the metal catalyst into powder, controlling the diameter of powder particles to be 8-10 mu m, and then putting the powder metal catalyst into purified water according to the proportion of 1: 9;
step two: stirring the mixed liquid in the step one for 30min, and quickly filtering the mixed liquid through a filter screen;
step three: injecting the filtered liquid into spraying equipment, and then spraying the specified detection area by using a spraying pot which is filled with catalytic liquid and is held by an experimenter wearing protective clothing, wherein the sprayed liquid contains a large amount of metal ions, and organophosphorus pesticide can be hydrolyzed into alcohol and phosphoric acid under the catalytic action of the metal ions;
step four: experimenters need to carry out two to three times of circulating spraying in a specified detection area, so that the uniformity of spraying coverage is guaranteed;
step five: before spraying, preparing a plurality of V-shaped card test paper, soaking the test paper clamping pieces in phenolphthalein solution, taking out the test paper clamping pieces, soaking the test paper clamping pieces in alkaline solution, and taking out the test paper after soaking for a period of time;
step six: placing the test paper clamping piece soaked and taken out in the step five into a vacuum drying device for rapid drying, controlling the drying temperature to be 55-60 ℃, and taking out the test paper after the moisture on the test paper is completely evaporated, and sealing and storing the test paper;
step seven: after spraying, clamping the test paper clamping piece on the surface of the plant stem and leaf sprayed with the catalyst, randomly selecting different areas for detection, then taking down the test paper clamping piece, observing the change of the surface color of the test paper, and judging the condition of pesticide residue;
step eight: and after the detection is finished, the plants in the detection area are subjected to spray irrigation and cleaning through clear water, and residual reagents and pesticide components on the surfaces of the plants are treated.
2. The method for detecting pesticide residue capable of quickly knowing a result according to claim 1, characterized in that: in the first step, the metal catalyst can be an electrolytic silver catalyst or a molten iron catalyst.
3. The method for detecting pesticide residue capable of rapidly knowing the result according to claim 1, which is characterized in that: in the fifth step, the phenolphthalein reagent and the alkaline reagent are colorless liquids, and when the test paper soaked in the phenolphthalein solution enters the alkaline reagent, the white test paper clip is changed into red.
4. The method for detecting pesticide residue capable of rapidly knowing the result according to claim 1, which is characterized in that: in the sixth step, after the white test paper clip is red, the white test paper clip is dried, redundant moisture in the test paper is evaporated, the test paper clip is restored to a dry state, and the test paper clip still keeps a red dominant appearance in the dry state.
5. The method for detecting pesticide residue capable of quickly knowing a result according to claim 1, characterized in that: in the seventh step, the red test paper clip is alkaline, and when the red test paper clip is contacted with the hydrolysis acid products on the stems and leaves, the red test paper clip is changed into colorless test paper from red, namely the original white state of the test paper.
6. The method for detecting pesticide residue capable of rapidly knowing the result according to claim 1, which is characterized in that: and seventhly, after the test paper clamping piece is taken down, the situation of pesticide residue in the area can be directly judged through a colorimetric method.
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| CN1266990A (en) * | 1999-03-12 | 2000-09-20 | 汪土新 | Reagent for speedily detecting residual agricultural organophosphorus chemicals |
| CN201773068U (en) * | 2010-03-12 | 2011-03-23 | 韩少春 | Multivariate test paper capable of testing pesticide residues of fruits and vegetables |
| CN105866117A (en) * | 2016-06-03 | 2016-08-17 | 四川大学 | Detection method for organophosphorus pesticide dimethoate residue based on test paper colorimetry |
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| WO2020063716A1 (en) * | 2018-09-26 | 2020-04-02 | 中国农业大学 | Detection kit and detection method for drift or deposition characteristic of sprayed droplet |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWM479417U (en) * | 2013-12-23 | 2014-06-01 | Brilliant Sensing Technology | Apparatus for residual pesticide detection |
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- 2020-08-20 CN CN202010845017.2A patent/CN111965177B/en active Active
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|---|---|---|---|---|
| CN1266990A (en) * | 1999-03-12 | 2000-09-20 | 汪土新 | Reagent for speedily detecting residual agricultural organophosphorus chemicals |
| CN201773068U (en) * | 2010-03-12 | 2011-03-23 | 韩少春 | Multivariate test paper capable of testing pesticide residues of fruits and vegetables |
| CN105866117A (en) * | 2016-06-03 | 2016-08-17 | 四川大学 | Detection method for organophosphorus pesticide dimethoate residue based on test paper colorimetry |
| CN108152095A (en) * | 2017-11-28 | 2018-06-12 | 辽宁出入境检验检疫局检验检疫技术中心 | Four kinds of bhc pesticide residue material standard samples and preparation method thereof in spinach |
| WO2020063716A1 (en) * | 2018-09-26 | 2020-04-02 | 中国农业大学 | Detection kit and detection method for drift or deposition characteristic of sprayed droplet |
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