CN111948167A - Method for quickly quantifying concentration of polystyrene microspheres and tetracycline in water - Google Patents

Method for quickly quantifying concentration of polystyrene microspheres and tetracycline in water Download PDF

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CN111948167A
CN111948167A CN202010831062.2A CN202010831062A CN111948167A CN 111948167 A CN111948167 A CN 111948167A CN 202010831062 A CN202010831062 A CN 202010831062A CN 111948167 A CN111948167 A CN 111948167A
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tetracycline
concentration
polystyrene microspheres
absorbance
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赵伟高
田一梅
赵鹏
崔丽敏
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Tianjin University
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    • GPHYSICS
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    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract

The invention discloses a method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water, belonging to the technical field of environmental pollution and chemical analysis, and comprising the following steps: diluting or concentrating a sample to be detected containing polystyrene microspheres and tetracycline by a certain multiple, determining the absorbance of the sample to be detected at a specific wavelength by using an ultraviolet-visible spectrophotometer, and calculating to obtain the concentrations of the polystyrene microspheres and the tetracycline in the sample to be detected according to an established calibration curve or regression equation between the absorbance and the concentrations of the polystyrene microspheres and the tetracycline. The invention provides a method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water under the condition that two pollutants exist simultaneously, and the method is convenient to detect and can be used for rapidly measuring mass samples in scientific research.

Description

Method for quickly quantifying concentration of polystyrene microspheres and tetracycline in water
Technical Field
The invention relates to the technical field of environmental pollution and chemical analysis, in particular to a method for quickly quantifying the concentration of polystyrene microspheres and tetracycline in water.
Background
With the increase of the global plastic usage, the problem of waste plastic in the environment is not negligible. The micro plastic is plastic particles with the size less than 5mm, has the characteristics of small size, large specific surface area and the like, can be used as a carrier of a plurality of pollutants in a water environment and can be diffused in the environment, so that the living of organisms in the water environment is damaged, and even the micro plastic enters a food chain to influence the health of a human body. In addition, organic pollutants in the water environment, such as antibiotics and the like, are also persistent pollutants which are harmful to the ecology and the human health.
The polystyrene microspheres and tetracycline serving as representative substances of micro plastic particles (colloid) and antibiotics (organic matters) can be used for researching the synergistic effect of two pollutants in a complex water environment in the presence of the two pollutants at the same time. At present, the concentration of polystyrene microspheres can be measured by a coulter particle technique instrument, a microscopic counting method, a turbidity method, an ultraviolet spectrophotometry and the like, and the concentration of tetracycline can be measured by a spectroscopic method, a microbiological method, a chromatography method, capillary electrophoresis and the like.
However, real water environments are complex, often with multiple contaminants present at the same time. Although the detection method can carry out quantification under the condition that a single pollutant exists, when two pollutants or multiple pollutants exist at the same time, rapid quantitative measurement cannot be carried out simultaneously due to mutual interference between the pollutants, the two or multiple pollutants are often required to be separated and then detected respectively, the operation is complex, and the price is high.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, provides a method for quickly quantifying the concentration of polystyrene microsphere microspheres and tetracycline in water aiming at the current situation that a method for quickly quantifying two pollutants simultaneously exists in water is not enough, and is used for testing the quantification of pollutants in the environmental field.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water, which comprises the steps of diluting or concentrating a sample to be tested containing the polystyrene microspheres and the tetracycline, measuring the absorbance of the diluted or concentrated sample to be tested at a specific wavelength by using an ultraviolet-visible spectrophotometer, and calculating to obtain the concentration of the polystyrene microspheres and the tetracycline in the sample to be tested according to an established calibration curve or regression equation between the absorbance and the concentration of the polystyrene microspheres and the tetracycline.
As a further improvement of the invention, the method for rapidly quantifying the concentration of the polystyrene microspheres and the tetracycline in the water specifically comprises the following steps:
(1) establishing a correction curve or regression equation
The first step is as follows: preparing polystyrene microsphere-tetracycline mixed suspension with known concentration C and C', diluting by different times, and adding a blank group into a background solution;
the second step is that: respectively measuring the absorbance of the mixed suspension at each concentration at the wavelength of 220nm and 360nm by an ultraviolet-visible spectrophotometer, averaging to obtain the absorbance A, A' of the mixed suspension at the concentration, and obtaining the absorbance A of the blank sampleIAnd AI'; and in the second step, the wavelengths 220nm and 360nm are absorption wavelengths determined after the two substances and the mixture thereof are subjected to absorption spectrum scanning, the absorbance of the measured substance at the wavelength is stable and easy to measure, and when the two substances are mixed, the interference of the other substance has no nonlinear effect on the measured substance.
The third step: according to absorbance A-AIWith A' -AI'drawing a correction curve with the concentration C and C' of the polystyrene microspheres and the tetracycline in the suspension, or performing linear regression to obtain a regression equation at the corresponding wavelength; if the correlation coefficient of the correction curve or the regression equation is higher than 0.99, the mutual interference effect of the two test pollutants in the ultraviolet spectrophotometry can be ignored, and the method is feasible;
(2) calculating the concentration of polystyrene microspheres and tetracycline
The first step is as follows: diluting or concentrating a sample to be detected containing the polystyrene microspheres and the tetracycline to obtain a diluted or concentrated suspension, and enabling the concentrations of the polystyrene microspheres and the tetracycline in the diluted or concentrated suspension to be in a linear range of a regression equation or a correction curve;
the second step is that: and (2) under the same conditions in the step (1), measuring the absorbance of the diluted or concentrated suspension at 220nm and 360nm by using an ultraviolet-visible spectrophotometer, and calculating the concentrations of the polystyrene microspheres and tetracycline in the sample to be measured according to a corresponding correction curve or a regression equation.
As a further improvement of the invention, the first step of step (1) is diluted by 1.25, 2, 3, 10, 12.5, 20, 33 and 100 times, respectively.
As a further improvement of the present invention, the polystyrene microspheres and tetracycline can be replaced with other contaminants including colloids and organics.
As a further improvement of the invention, the other contaminants include micro plastic particles, clay particles, engineered nanoparticles or humic acid.
As a further improvement of the present invention, when the polystyrene microspheres and tetracycline are replaced with other contaminants, the corresponding test absorbances are replaced with the corresponding absorbances of the contaminants tested from 220nm and 360 nm.
The invention discloses the following technical effects:
(1) the invention provides a novel test method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water, compared with the prior quantification technology, the method can realize the simultaneous quantification and concentration comparison of the polystyrene microspheres and the tetracycline in the water environment, does not need separation, and has simple operation, high efficiency and convenience;
(2) the invention provides a new idea of a rapid quantification method when two or more pollutants coexist in a complex water environment, if different pollutants can be measured by a spectrophotometry method and are calculated by a correction curve or a regression equation, the mutual interference effect can be ignored, and complex calculation is not needed for eliminating the influence of the mutual interference.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a calibration curve of absorbance versus concentration of polystyrene microspheres, tetracycline, polystyrene microspheres-tetracycline in example 1;
FIG. 2 is a calibration curve for absorbance and concentration of polystyrene microsphere suspensions, tetracycline solutions, polystyrene microsphere-tetracycline mixed suspensions at wavelength 220nm and at wavelength 360nm in example 1;
FIG. 3 is a calibration curve of the calculated absorbance versus the measured absorbance for the polystyrene microsphere-tetracycline mixed suspension of example 1;
FIG. 4 is the absorption spectra of the polystyrene microsphere suspension, tetracycline solution, and mixed suspension of polystyrene microsphere-tetracycline in example 2 at different concentrations (a), (b), (c), and (d) respectively for MPs +20mg/L TC, MPs, and TC stock solution diluted 1.25, 2, 3.33, 10, 12.5, 20, 33.3, and 100 times;
FIG. 5 is a graph of the filtration penetration of polystyrene microspheres and tetracycline over time at different ionic strengths from example 3;
FIG. 6 is a calibration curve of absorbance versus concentration of polystyrene microspheres, tetracycline, polystyrene microspheres-tetracycline in example 4;
FIG. 7 is a graph of the filtration breakthrough of polystyrene microspheres and tetracycline over time in different tetracycline concentration environments of example 4.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The invention relates to a method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water, which comprises the steps of diluting or concentrating a sample to be tested containing the polystyrene microspheres and the tetracycline by a certain multiple, measuring the absorbance of the sample to be tested at a specific wavelength by using an ultraviolet-visible spectrophotometer, and calculating to obtain the concentration of the polystyrene microspheres and the tetracycline in the sample to be tested according to an established calibration curve or regression equation between the absorbance and the concentration of the polystyrene microspheres and the tetracycline.
(1) Establishing a correction curve or regression equation
The first step is as follows: preparing a certain amount of polystyrene microsphere-tetracycline mixed suspension with known concentration C and C', respectively diluting by 1.25, 2, 3, 10, 12.5, 20, 33 and 100 times, and adding a background solution into a blank group;
the second step is that: respectively measuring the absorbance of the mixed suspension at each concentration at the wavelength of 220nm and 360nm by an ultraviolet-visible spectrophotometer, averaging to obtain the absorbance A, A' of the mixed suspension at the concentration, and obtaining the absorbance A of the blank sampleIAnd AI'. The wavelengths of 220nm and 360nm are absorption wavelengths determined after the absorption spectrum scanning of the two substances and the mixture thereof. At the wavelength, the absorbance of the substance to be measured is stable and easy to measure, and when the two substances are mixed, the interference of the other substance has no nonlinear effect on the substance to be measured. The polystyrene microspheres and the tetracycline can be replaced by other pollutants (including colloids, organic matters and the like), such as micro plastic particles, clay particles, engineering nanoparticles, humic acid and the like, and at the moment, the corresponding test absorbance is replaced by the corresponding absorbance of the pollutants to be tested from 220nm and 360 nm;
the third step: according to absorbance A-AIWith A' -AI'and the concentration C and C' of the polystyrene microspheres and the tetracycline in the suspension are used for drawing a calibration curve, or linear regression is carried out to obtain a regression equation at the corresponding wavelength. If the correlation coefficient of the calibration curve or the regression equation is higher than 0.99, the mutual interference of the two tested pollutants in the ultraviolet spectrophotometry can be ignored, and the method is feasible.
(2) Calculating the concentration of polystyrene microspheres and tetracycline
The first step is as follows: diluting or concentrating a sample to be detected containing the polystyrene microspheres and the tetracycline, so that the concentrations of the polystyrene microspheres and the tetracycline in the diluted or concentrated suspension are in a linear range of a regression equation or a correction curve;
the second step is that: and (2) under the same conditions in the step (1), measuring the absorbance of the diluted or concentrated suspension at 220nm and 360nm by using an ultraviolet-visible spectrophotometer, and calculating the concentrations of the polystyrene microspheres and tetracycline in the sample to be measured according to a corresponding correction curve or a regression equation.
The test method for rapidly quantifying the concentration of the polystyrene microspheres and the tetracycline in the water is used for scientific research in the fields of environmental pollution and the like, and has important significance for scientific research on the condition that two or more pollutants exist in a complex water environment at the same time and understanding the synergistic migration and transformation of multiple pollutants in the environment.
Example 1
The invention relates to a method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water, which comprises the steps of diluting or concentrating a sample to be tested containing the polystyrene microspheres and the tetracycline by a certain multiple, measuring the absorbance of the sample to be tested at a specific wavelength by using an ultraviolet-visible spectrophotometer, and calculating to obtain the concentration of the polystyrene microspheres and the tetracycline in the sample to be tested according to an established calibration curve or regression equation between the absorbance and the concentration of the polystyrene microspheres and the tetracycline.
1. Preparation of standard suspension: 50mL of polystyrene microspheres with a concentration of 5.69X 10 were prepared7The mixed suspension of polystyrene Microspheres (MS) -Tetracycline (TC) with tetracycline concentration of 20mg/L is diluted by 1.25, 2, 3, 10, 12.5, 20, 33 and 100 times respectively, and the blank group is added with the background solution. As a control, polystyrene microsphere suspensions of the same concentration C and tetracycline solutions of the same concentration C' were prepared and diluted 1.25, 2, 3, 10, 12.5, 20, 33, 100 times, respectively.
2. Measurement of absorbance: respectively measuring the absorbance of the mixed suspension at each concentration at the wavelengths of 220nm and 360nm by using an ultraviolet-visible spectrophotometer, taking the average value of 3 parallel samples in each group to obtain the absorbance A, A' of the mixed suspension at the concentration, and obtaining the absorbance A of a blank sampleIAnd AI'. As a control, the absorbance A of the polystyrene microsphere suspension at a wavelength of 220nm was measured0And the absorbance A of the tetracycline solution at a wavelength of 360nm0'. In addition, in order to investigate whether the two mixed solutions have nonlinear interference effect on absorbance, the absorbance A of the polystyrene microsphere suspension at 360nm wavelength was measured respectively2And the absorbance A of the tetracycline solution at a wavelength of 220nm2’。
3. Establishment of a linear relationship: and obtaining a correction curve according to the absorbance A, A 'measured in the mixed suspension and the concentrations C and C' of the polystyrene microspheres and the tetracycline in the mixed suspension, wherein the correction curve has a good linear relation. As a control, the absorbance A was measured according to the polystyrene microsphere suspension and the tetracycline solution0、A0', the concentration C and C' of the polystyrene microsphere suspension and the tetracycline solution, and a calibration curve is obtained, and the calibration curve has a good linear relation. According to the absorbance A of the polystyrene suspension at the wavelength of 360nm2And adsorption of the tetracycline solution at a wavelength of 220nmLuminosity A2' to obtain a correction curve with good linear relation. The results are shown in table 1, fig. 1, and fig. 2.
TABLE 1 Linear relationship between absorbance and concentration of polystyrene microspheres, tetracycline
Figure BDA0002637996130000091
Wherein y is the photometric quantity A-A at the corresponding wavelength measured by an ultraviolet-visible spectrophotometerI、A’-AI’、A0-AI、A0’-AIX is polystyrene microsphere, and the concentration of tetracycline C and C' are unit/ml, mg/L, R2Is a linear correlation coefficient.
As can be seen from FIG. 1 and Table 1, when the concentration of polystyrene microspheres is 105-108The tetracycline concentration is within the range of 0-20mg/L in the range of one/mL, and the linear correlation coefficients of the absorbance and the concentration in single and mixed suspensions are both more than 0.999, which indicates that the regression equation has good linear relation.
FIG. 2 shows the absorbance and concentration calibration curves of polystyrene microsphere suspension, tetracycline solution and polystyrene microsphere-tetracycline mixed suspension at wavelength 220nm and wavelength 360nm, the concentrations and absorbances of polystyrene microsphere, tetracycline and polystyrene microsphere-tetracycline are highly correlated on two y-axes, and the linear correlation coefficients are all above 0.999. FIG. 3 is a relationship between a calculated absorbance value and a measured absorbance value in a mixed suspension obtained by using a single-term addition method, showing a correlation addition between the measured absorbance (polystyrene microsphere-tetracycline mixed suspension) and the calculated single-term absorbance of the polystyrene microsphere suspension and the tetracycline solution, indicating that mutual interference of two test pollutants in an ultraviolet spectrophotometry has no nonlinear effect and can be ignored, and the method is feasible.
Therefore, the regression equation obtained in Table 1 and FIG. 1 of the present method is suitable for the rapid quantification of the concentrations of polystyrene microspheres and tetracycline in the mixed solution.
Example 2
According to the test method for rapidly quantifying the concentration of the polystyrene microspheres and the tetracycline in the water, the mutual influence of the polystyrene microspheres and the tetracycline in coexistence under different concentration conditions is explored, and the feasibility of the method is verified. The method comprises the following specific steps:
1. preparation of standard suspension: respectively preparing a certain quantity of polystyrene microspheres with the concentration of 5.69 multiplied by 107Mixing each/mL with polystyrene Microsphere (MS) -Tetracycline (TC) mixed suspension with tetracycline concentration of 1, 5, 10, 20mg/L, diluting respectively by 1.25, 2, 3, 10, 12.5, 20, 33, 100 times, and adding the blank group into the background solution. As a control, polystyrene microsphere suspensions of the same concentration C and tetracycline solutions of the same concentration C' were prepared and diluted 1.25, 2, 3, 10, 12.5, 20, 33, 100 times, respectively.
2. Measurement of absorbance: respectively measuring the absorbance of the mixed suspension at each concentration at the wavelengths of 220nm and 360nm by using an ultraviolet-visible spectrophotometer, taking the average value of 3 parallel samples in each group to obtain the absorbance A, A' of the mixed suspension at the concentration, and obtaining the absorbance A of a blank sampleIAnd AI'. As a control, the absorbance A of the polystyrene microsphere suspension at a wavelength of 220nm was measured0And the absorbance A of the tetracycline solution at a wavelength of 360nm0’。
3. Full wavelength scanning: the scanning wave band is set to be 200-800nm, the scanning speed is medium speed, and the scanning interval is 1 nm. After the baseline scan was performed, each sample was placed for scanning. And scanning to obtain an absorbance value-wavelength relation curve, namely a spectrum curve of the sample. The results are shown in FIG. 4.
As can be seen from FIG. 4, under any condition, the polystyrene microspheres and tetracycline have characteristic peaks at the measurement wavelengths of 220nm and 360nm, and have good linear relationship, and the mutual interference of the two substances has no nonlinear effect.
Example 3
According to the method for rapidly quantifying the concentration of the polystyrene microspheres and the tetracycline in the water, the filtering effect of the same filter material in the coexistence of the polystyrene microspheres and the tetracycline in the environments with different ionic strengths is researched, and the method comprises the following specific steps:
1. preparation of mixed suspension: the prepared polystyrene microsphere has the concentration of 5.69 multiplied by 107Filtration experiments were performed on polystyrene Microsphere (MS) -Tetracycline (TC) mixed suspensions of 20 mg/mL tetracycline, 20mg/L tetracycline, and 1mM KCl and 50mM KCl, respectively, as the background solution ionic strength.
2. Filtration experiment: and (3) performing wet filling by using a filter column with the inner diameter of 16mm, wherein the thickness of a filter layer is 15.0 +/-0.1 cm, and performing a filtration experiment after the filter column is filled. The filtration flow rate is 1.5M/h, the pH is 7.0. + -. 0.5, the environment is room temperature, and the detailed experimental procedures can be referred to [ Jin C, Normani S D, Emelko M B. surface routes images on granular media positioning conditions: Experiments and modeling [ J ]. Environmental science & technology,2015,49(13): 7879-. And (3) investigating the filtering effect of the same filter material on the coexistence of the polystyrene microspheres and the tetracycline under different ionic strengths.
3. Measurement of absorbance in filtration experiment: samples were taken over time and the absorbance at wavelengths 220nm and 360nm of the filtered mixed suspension samples was determined by UV-visible spectrophotometer. The concentration was calculated by the linear relationship established in example 1 between polystyrene microspheres, tetracycline concentration and absorbance in the mixed suspension at this concentration. The results are shown in FIG. 5.
From fig. 5, the concentration of the polystyrene microspheres and the tetracycline measured by the method can reflect the filtering and removing effects of the same filter material on the coexistence of two substances under different ionic strengths. Inorganic anionic Cl in higher and lower salinity environments-The ultraviolet photometry of the polystyrene microspheres and the tetracycline is almost free of interference. When a large number of samples need to be measured, the method is convenient to operate and simple and easy to implement.
Example 4
According to the test method for rapidly quantifying the concentration of the polystyrene microspheres and the tetracycline in the water, provided by the invention, a calibration curve or a regression equation between the absorbance of the polystyrene microspheres and the tetracycline and the concentration of the polystyrene microspheres and the tetracycline under different concentration conditions is established, and the filtering effect of the same filter material on the coexistence of the polystyrene microspheres and the tetracycline in different tetracycline concentration environments is researched. The method comprises the following specific steps:
1. preparation of mixed suspension: the prepared polystyrene microsphere has the concentration of 5.69 multiplied by 107Polystyrene Microsphere (MS) -Tetracycline (TC) mixed suspension with tetracycline concentration of 1mg/L and 10mg/L and background solution ionic strength of 100mM KCl respectively. The diluted solutions are respectively diluted by 1.25, 2, 3, 10, 12.5, 20, 33 and 100 times, and the blank group is added with the background solution.
2. Measurement of absorbance: and respectively measuring the absorbance of the mixed suspension with each concentration at the wavelengths of 220nm and 360nm by using an ultraviolet-visible spectrophotometer, taking the average value of 3 parallel samples in each group to obtain the absorbance of the mixed suspension with the concentration, and measuring the absorbance of a blank sample.
3. Establishment of a linear relationship: and (3) according to the measured absorbance and the concentrations of the polystyrene microspheres and the tetracycline in the mixed suspension, obtaining a correction curve, and having a good linear relation. The results are shown in table 2 and fig. 6.
4. Filtration experiment: and (3) performing wet filling by using a filter column with the inner diameter of 16mm, wherein the thickness of a filter layer is 15.0 +/-0.1 cm, and performing a filtration experiment after the filter column is filled. The filtration flow rate is 1.5M/h, the pH is 7.0. + -. 0.5, the environment is room temperature, and the detailed experimental procedures can be referred to [ Jin C, Normani S D, Emelko M B. surface routes images on granular media positioning conditions: Experiments and modeling [ J ]. Environmental science & technology,2015,49(13): 7879-. And (3) investigating the filtering effect of the same filter material on the coexistence of the polystyrene microspheres and the tetracycline in different tetracycline concentration environments.
5. Measurement of absorbance in filtration experiment: samples were taken over time and the absorbance at wavelengths 220nm and 360nm of the filtered mixed suspension samples was determined by UV-visible spectrophotometer. The concentration was calculated from the linear relationship between polystyrene microspheres, tetracycline concentration and absorbance in the mixed suspension at the different concentrations described above. The results are shown in FIG. 7.
TABLE 2 Linear relationship between absorbance and concentration of polystyrene microspheres, tetracycline
Figure BDA0002637996130000131
Wherein y is the photometric value at the corresponding wavelength measured by an ultraviolet-visible spectrophotometer, and x is the concentration of polystyrene microspheres and tetracycline, and the unit is unit/ml, mg/L, R2Is a linear correlation coefficient.
From fig. 7, the concentration of the polystyrene microspheres and the tetracycline determined by the method can reflect the filtering and removing effects of the same filter material on the coexistence of two substances in different tetracycline concentration environments. When a large number of samples need to be measured, the method is convenient to operate and simple and easy to implement.
The invention can be used for researching the behavior of pollutants when water environment colloid and organic matters exist simultaneously. In the filtration experiment, the method is used for measuring the concentration of the pollutants in the filtered water sample, so that the mechanism analysis and research of the migration behavior of the pollutants are carried out. In other fields of research, the method can be used when concentration measurements are required in the presence of two colloids or organics.
Although the method and assay of the present invention are described in the specification with reference to the drawings, the present invention is not limited to the specific test procedures and contaminants described above, and the specific embodiments described above are illustrative only and not limiting. Equivalents may be made without departing from the spirit and scope of the invention as defined in the claims, and are not excluded from the scope of the invention.

Claims (6)

1. A method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water is characterized in that a sample to be tested containing the polystyrene microspheres and the tetracycline is diluted or concentrated, the absorbance of the diluted or concentrated sample to be tested at a specific wavelength is measured by an ultraviolet-visible spectrophotometer, and the concentration of the polystyrene microspheres and the tetracycline in the sample to be tested is obtained by calculation according to an established calibration curve or regression equation between the absorbance and the concentration of the polystyrene microspheres and the tetracycline.
2. The method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water according to claim 1, comprising the steps of:
(1) establishing a correction curve or regression equation
The first step is as follows: preparing polystyrene microsphere-tetracycline mixed suspension with known concentration C and C', diluting by different times, and adding a blank group into a background solution;
the second step is that: respectively measuring the absorbance of the mixed suspension at each concentration at the wavelength of 220nm and 360nm by an ultraviolet-visible spectrophotometer, averaging to obtain the absorbance A, A' of the mixed suspension at the concentration, and obtaining the absorbance A of the blank sampleIAnd AI’;
The third step: according to absorbance A-AIWith A' -AI'drawing a correction curve with the concentration C and C' of the polystyrene microspheres and the tetracycline in the suspension, or performing linear regression to obtain a regression equation at the corresponding wavelength;
(2) calculating the concentration of polystyrene microspheres and tetracycline
The first step is as follows: diluting or concentrating a sample to be detected containing the polystyrene microspheres and the tetracycline to obtain a diluted or concentrated suspension, and enabling the concentrations of the polystyrene microspheres and the tetracycline in the diluted or concentrated suspension to be in a linear range of a regression equation or a correction curve;
the second step is that: and (2) under the same conditions in the step (1), measuring the absorbance of the diluted or concentrated suspension at 220nm and 360nm by using an ultraviolet-visible spectrophotometer, and calculating the concentrations of the polystyrene microspheres and tetracycline in the sample to be measured according to a corresponding correction curve or a regression equation.
3. The method for rapidly quantifying the concentration of polystyrene microspheres and tetracycline in water according to claim 2, wherein the first step of step (1) is dilution by 1.25, 2, 3, 10, 12.5, 20, 33 and 100 times, respectively.
4. The method of claim 1, wherein the polystyrene microspheres and tetracycline are replaced with other contaminants including colloids and organics.
5. The method of claim 4, wherein the other contaminants comprise micro plastic particles, clay particles, engineered nanoparticles, or humic acid.
6. The method of claim 5, wherein when polystyrene microspheres and tetracycline are replaced with other contaminants, the corresponding test absorbance is replaced with the corresponding absorbance of the contaminant being tested from 220nm and 360 nm.
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CN117309793B (en) * 2023-10-25 2024-05-07 连云港石化有限公司 Method for measuring polymer of styrene device

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