CN111940005A - Test tube rack for biological cell research and application thereof - Google Patents
Test tube rack for biological cell research and application thereof Download PDFInfo
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- CN111940005A CN111940005A CN202010884373.5A CN202010884373A CN111940005A CN 111940005 A CN111940005 A CN 111940005A CN 202010884373 A CN202010884373 A CN 202010884373A CN 111940005 A CN111940005 A CN 111940005A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The utility model provides a test-tube rack for biological cell research and application thereof, relates to biological cell technical field, including the coil, the inside swing joint of coil has outer mounting panel, the inboard fixedly connected with test tube ring of outer mounting panel. This a test-tube rack for biological cell research and application thereof, through the advantage of overspeed self-stop, can utilize self structure to make the centrifugal process automatic stop of test tube when centrifugal device's rotational speed exceeds the rated value, prevents the problem of the cell tissue sample damage that causes because of centrifugal force is too big, prevents that cell tissue from being destroyed, can not cause the deactivation of cell tissue because of the stall even more. The validity of the centrifuged cell tissue sample is improved, the authenticity of experimental data is guaranteed, the reliability of the experimental result is improved, in addition, the problem that the experimental process is delayed due to the inactivation of the cell tissue can be avoided, certain time and cell tissue resources are saved, and the requirement of biological cell research is better met.
Description
Technical Field
The invention relates to the technical field of biological cells, in particular to a test tube rack for biological cell research and application thereof.
Background
The biological cell technology is an important part of the biological technology, and the research results and the research field of the biological cell technology lay a solid foundation for the development of modern biological science, thereby not only directly promoting the process of biological research, but also providing scientific basis for the development of other related fields.
In biological cell research, components of cells are often analyzed and identified, and a centrifugal machine is usually needed to perform centrifugal operation on a cell tissue sample, so that different cell tissues are layered, and the cell tissues are conveniently extracted. The test tube rack of the existing centrifuge is directly connected with the driving device, namely the rotating speed of the test tube rack is the same as that of the driving device. Since the driving device is generally controlled by a motor, when the device is used, the rotating speed of the motor may stall due to program or mechanical failure, and the rotating speed of the test tube rack exceeds a normal rated value. When the cell sample in the centrifugation exceeds the rated centrifugal force, the cell sample can cause irreversible damage to the tissue of the cell, destroy the tissue components of the cell, and seriously even cause the inactivation of the cell. The above problems not only affect the validity of the tissue sample, resulting in reduced authenticity and reliability of experimental data, but also delay progress of experimental process and research, resulting in a great amount of time and resource waste.
In order to solve the above problems, the inventors propose a test tube rack for biological cell research and an application thereof, which have the advantage of overspeed self-stop.
Disclosure of Invention
In order to achieve the purpose of overspeed self-stopping, the invention provides the following technical scheme: a test tube rack for biological cell research and application thereof comprise a coil, an outer mounting plate, a test tube ring, a conductor, a clamping mechanism, an inner mounting plate, a driving mechanism, a positioning groove, a positioning ring, a limiting column, a limiting spring, a driving shaft, a central shaft, a frame body, a reset spring, a clamping block and an electromagnetic device.
The position and connection relation of the structure is as follows:
the inner side of the test tube ring is fixedly connected with an inner mounting plate, the inner side of the inner mounting plate is movably connected with a driving mechanism, and a positioning groove is formed between the outer mounting plate and the inner mounting plate;
the clamping mechanism comprises a positioning ring, the outer side of the positioning ring is connected with a limiting column in a prescribed mode, and the inner side of the positioning ring is fixedly connected with a limiting spring;
the driving mechanism comprises a driving shaft, a central shaft is fixedly connected to the inside of the driving shaft, a frame body is fixedly connected to the outer side of the central shaft, a return spring is fixedly connected to the inside of the frame body, a clamping block is fixedly connected to the outer side of the return spring, and an electromagnetic device is arranged inside the central shaft.
Preferably, in an initial state, the four clamping blocks correspond to the four clamping grooves in the inner side of the inner mounting plate respectively, and the clamping blocks are located in the clamping grooves and are in inserted connection.
Preferably, one end, far away from the positioning ring, of the limiting column is located inside the test tube ring, one end, far away from the positioning ring, of the limiting column is provided with an anti-falling block, and the size of the anti-falling block is larger than that of the limiting column.
Preferably, the clamping mechanism in the same group comprises two limiting springs with the same specification, the two limiting springs are respectively and fixedly connected with one sides of the two positioning rings in the same group, which are opposite to each other, and the two limiting springs are distributed in parallel.
Preferably, the driving shaft is fixedly connected with an output shaft of the external motor.
Preferably, the inner side end of the fixture block is provided with a block structure, the inner side of one end of the frame body away from the central shaft is provided with a protrusion, and the size of the block structure is smaller than that of the protrusion.
Advantageous effects
Compared with the prior art and products, the invention has the beneficial effects that:
1. this a test-tube rack for biological cell research and application thereof can be when equipment is in normal rotational speed, does not influence its normal use, guarantees centrifugal equipment's normal work, does not cause extra influence and burden to equipment, and the operability is higher and more reasonable.
2. This a test-tube rack for biological cell research and application thereof, through the advantage of overspeed self-stop, can utilize self structure to make the centrifugal process automatic stop of test tube when centrifugal device's rotational speed exceeds the rated value, prevents the problem of the cell tissue sample damage that causes because of centrifugal force is too big, prevents that cell tissue from being destroyed, can not cause the deactivation of cell tissue because of the stall even more. The validity of the centrifuged cell tissue sample is improved, the authenticity of experimental data is guaranteed, the reliability of the experimental result is improved, in addition, the problem that the experimental process is delayed due to the inactivation of the cell tissue can be avoided, certain time and cell tissue resources are saved, and the requirement of biological cell research is better met.
Drawings
FIG. 1 is a first schematic view of a connection structure according to the present invention;
FIG. 2 is a schematic diagram of the movement traces of the structures in FIG. 1 according to the present invention;
FIG. 3 is a schematic view of the connection structure of the clamping mechanism of the present invention;
FIG. 4 is a schematic view of the connection structure of the driving mechanism of the present invention;
FIG. 5 is a schematic diagram of the movement traces of the structures shown in FIG. 4 according to the present invention;
FIG. 6 is a schematic view of a coil structure according to the present invention;
FIG. 7 is a second schematic view of a connection structure according to the present invention;
fig. 8 is a schematic diagram of the motion traces of the structures in fig. 7 according to the present invention.
In the figure: 1. a coil; 2. an outer mounting plate; 3. a test tube ring; 4. a conductor; 5. a clamping mechanism; 6. an inner mounting plate; 7. a drive mechanism; 8. positioning a groove; 51. a positioning ring; 52. a limiting column; 53. a limiting spring; 71. a drive shaft; 72. a central shaft; 73. a frame body; 74. a return spring; 75. a clamping block; 76. an electromagnetic device;
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Please refer to fig. 1-8:
the test tube rack for biological cell research and the application thereof comprise a coil 1, an outer mounting plate 2, a test tube ring 3, a conductor 4, a clamping mechanism 5, an inner mounting plate 6, a driving mechanism 7, a positioning groove 8, a positioning ring 51, a limiting column 52, a limiting spring 53, a driving shaft 71, a central shaft 72, a frame 73, a return spring 74, a fixture block 75 and an electromagnetic device 76.
The initial positions and the connection relations of the structures are as follows:
the inside of the coil 1 is movably connected with an outer mounting plate 2, the inner side of the outer mounting plate 2 is fixedly connected with a test tube ring 3, the front surface of the outer mounting plate 2 is fixedly connected with a conductor 4, the inside of the test tube ring 3 is provided with a clamping mechanism 5, the inner side of the test tube ring 3 is fixedly connected with an inner mounting plate 6, the inside of the inner mounting plate 6 is movably connected with a driving mechanism 7, and a positioning groove 8 is formed between the outer mounting plate 2 and the inner mounting plate 6;
the clamping mechanism 5 comprises a positioning ring 51, the outer side of the positioning ring 51 is connected with a limiting column 52 in a prescribed way, and the inner side of the positioning ring 51 is fixedly connected with a limiting spring 53;
the driving mechanism 7 includes a driving shaft 71, a central shaft 72 is fixedly connected to the inside of the driving shaft 71, a frame 73 is fixedly connected to the outside of the central shaft 72, a return spring 74 is fixedly connected to the inside of the frame 73, a latch 75 is fixedly connected to the outside of the return spring 74, and an electromagnetic device 76 is provided inside the central shaft 72.
Wherein:
a. the coil 1 comprises a bracket and a wire body, wherein the bracket is annular and is provided with ten uniformly distributed winding blocks, the wire body mainly comprises an enameled wire, a coating coated on the outer side of the enameled wire is required to be free of damage, and the wire body of the coil 1 is a complete enameled wire; the coil 1 has a current passing therethrough and magnetic induction lines, and the conductor 4 is perpendicular to the magnetic induction lines of the coil 1. In the initial state, the four locking blocks 75 respectively correspond to the four locking slots on the inner side of the inner mounting plate 6, and the locking blocks 75 are located inside the locking slots and are inserted into each other.
b. Outer mounting panel 2, test tube ring 3 and interior mounting panel 6 are concentric distribution, and constant head tank 8 is located outer mounting panel 2, test tube ring 3 and interior mounting panel 6's inside, is provided with ten and specification homogeneous phases altogether, and ten constant head tanks 8 are evenly distributed for referring to the centre of a circle of mounting panel 2 beyond for. The limiting column 52 is far away from one end of the positioning ring 51 and is positioned inside the test tube ring 3, and one end of the limiting column 52, which is far away from the positioning ring 51, is provided with an anti-falling block, and the size of the anti-falling block is larger than that of the limiting column 52.
c. The conductors 4 are provided with ten conductors which have the same specification and are uniformly distributed; ten positioning grooves 8 are respectively positioned among the ten conductors 4; the centers of the ten conductors 4 are on the same circle, and the distance and the angle between two adjacent conductors 4 are the same; the conductors 4 are electrically connected in series. The clamping mechanism 5 of the same group comprises two limiting springs 53 with the same specification, the two limiting springs 53 are respectively and fixedly connected with one side of the same group opposite to the two positioning rings 51, and the two limiting springs 53 are distributed in parallel.
d. Ten clamping mechanisms 5 are arranged, have the same specification and are uniformly distributed by taking the test tube ring 3 as a reference; ten clamping mechanisms 5 correspond to the ten positioning grooves 8, respectively, and the midpoints of the clamping mechanisms 5 are located on the center lines of the positioning grooves 8.
Wherein:
e. the same group of positioning rings 51 comprises two snap ring structures which are symmetrically distributed and have the same specification, the middle parts of the snap rings are circular, and the inner sides of the snap rings are made of rubber materials with larger friction coefficients; the two limiting columns 52 in the same group correspond to the two positioning rings 51 in the same group respectively, and are fixedly connected to the opposite sides of the two positioning rings 51 respectively, and the central lines of the two positioning rings are the same straight line. The driving shaft 71 is fixedly connected with an output shaft of the external motor.
f. The driving shaft 71 is concentric with the central shaft 72, four frame bodies 73, four return springs 74 and four latch blocks 75 are provided and respectively correspond to each other, the four frame bodies 73, the four return springs 74 and the four latch blocks 75 are identical in specification and size, the corresponding frame bodies 73, the four return springs 74 and the four latch blocks 75 are provided in a group, four groups are provided in total, and the four groups are uniformly distributed by taking the central shaft 72 as a reference.
g. The four electromagnetic devices 76 are arranged and have the same specification, and the four electromagnetic devices 76 are respectively and electrically connected with the coil 1 and the conductor 4; the fixture block 75 is made of a magnetic material, the magnetic pole is an N pole, four clamping grooves are formed in the inner side of the inner mounting plate 6, and the four clamping grooves are the same in specification and size and correspond to the four fixture blocks 75 respectively. The inner end of the latch 75 is provided with a block structure, and the inner side of the end of the frame 73 away from the central shaft 72 is provided with a protrusion, and the size of the block structure is smaller than that of the protrusion.
h. The four electromagnetic devices 76 respectively correspond to the four fixture blocks 75, and the electromagnetic devices 76 generate magnetism under rated current intensity and have S-shaped magnetic poles; the four electromagnetic devices 76 are spaced from the corresponding fixture block 75 by the same distance, and the centers of the four electromagnetic devices 76 are on the same circle.
When the test tube fixing device is used, a test tube filled with a biological cell sample is inserted between the two positioning rings 51, the two positioning rings 51 can clamp and fix the test tube by utilizing the tension action of the limiting spring 53, and the driving shaft 71 is connected with the output shaft of the external motor and starts the motor.
When the driving shaft 71 rotates, it can drive the structure inside and connected with it to rotate synchronously and in the same direction. Under the initial condition, because the inboard of interior mounting panel 6 is provided with four draw-in grooves, the specification and size of draw-in groove is the same and correspond with four fixture blocks 75 respectively, so can drive interior mounting panel 6 synchronous syntropy when drive shaft 71 rotates and rotate, and on the same principle, interior mounting panel 6 can drive the outer mounting panel 2, test tube ring 3, conductor 4, clamping mechanism 5 and the synchronous rotation of constant head tank 8 rather than being connected. When the clamping mechanism 5 rotates, the test tube which is clamped inside and is provided with the biological cell sample can be driven to synchronously rotate, and the biological cell sample is subjected to centrifugal operation.
In the process, the outer mounting plate 2 drives the conductor 4 to rotate, and because current passes through the coil 1 and magnetic induction lines exist in the coil, the conductor 4 is perpendicular to the magnetic induction lines of the coil 1, the conductor 4 cuts the magnetic induction lines of the coil 1 and generates induced current. Under normal rotation speed, the induction current value generated by the magnetic induction line of the conductor 4 cutting coil 1 is not enough to reach the rated current value of the electromagnetic device 76, so that the electromagnetic device 76 does not generate magnetic force at the moment, the fixture block 75 is at the initial position under the action of the return spring 74, namely, is clamped with the clamping groove formed in the inner side of the inner mounting plate 6, at the moment, the test tube ring 3 normally rotates, and the test tube filled with biological cell tissues in the clamping mechanism 5 continues to be centrifuged.
When the rotating speed of the driving shaft 71 is increased, the rotating speeds of the inner mounting plate 6, the outer mounting plate 2, the test tube ring 3, the conductor 4, the clamping mechanism 5 and the positioning groove 8 are driven to be synchronously increased, at the moment, the speed and the frequency of the conductor 4 for cutting the coil 1 are increased, and the generated induction current value is synchronously increased. When the induced current value reaches the rated current value of the electromagnetic device 76, the electromagnetic device 76 is started and generates magnetism, because the electromagnetic device 76 generates magnetism under the rated current intensity and the magnetic pole is S pole, the fixture block 75 is made of magnetic material and the magnetic pole is N pole, at the moment, the electromagnetic device 76 attracts the fixture block 75 by utilizing the principle of opposite attraction, so that the fixture block is separated from the clamping groove on the inner side of the inner mounting plate 6, at the moment, the driving shaft 71 does not drive the inner mounting plate 6 to rotate any more, similarly, when the mounting plate 6 stops, the clamping mechanism 5 connected with the mounting plate stops synchronously, and the test tube which is clamped inside and is provided with the biological cell tissue stops centrifuging.
The above structure and process are shown in FIGS. 1-8.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A test-tube rack for biological cell research and application thereof, including coil (1), characterized in that: an outer mounting plate (2) is movably connected inside the coil (1), a test tube ring (3) is fixedly connected to the inner side of the outer mounting plate (2), a conductor (4) is fixedly connected to the front side of the outer mounting plate (2), a clamping mechanism (5) is arranged inside the test tube ring (3), an inner mounting plate (6) is fixedly connected to the inner side of the test tube ring (3), a driving mechanism (7) is movably connected inside the inner mounting plate (6), and a positioning groove (8) is formed between the outer mounting plate (2) and the inner mounting plate (6);
the clamping mechanism (5) comprises a positioning ring (51), the outer side of the positioning ring (51) is connected with a limiting column (52) in a prescribed manner, and the inner side of the positioning ring (51) is fixedly connected with a limiting spring (53);
the driving mechanism (7) comprises a driving shaft (71), a central shaft (72) is fixedly connected inside the driving shaft (71), a frame body (73) is fixedly connected to the outer side of the central shaft (72), a return spring (74) is fixedly connected inside the frame body (73), a clamping block (75) is fixedly connected to the outer side of the return spring (74), and an electromagnetic device (76) is arranged inside the central shaft (72).
2. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: the coil (1) comprises a support and a wire body, the support is annular and is provided with ten uniformly distributed winding blocks, the wire body mainly comprises an enameled wire, the enamel coating coated on the outer side of the enameled wire is required to be free of damage, and the wire body of the coil (1) is a complete enameled wire; current passes through the coil (1) and magnetic induction lines exist in the coil, and the conductor (4) is perpendicular to the magnetic induction lines of the coil (1).
3. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: outer mounting panel (2), test tube ring (3) and interior mounting panel (6) are concentric and distribute, constant head tank (8) are located the inside of outer mounting panel (2), test tube ring (3) and interior mounting panel (6), and it is the same to be provided with ten and specification altogether, and the centre of a circle of mounting panel (2) is evenly distributed for referring to outside ten constant head tanks (8).
4. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: the conductors (4) are provided with ten conductors which have the same specification and are uniformly distributed; the ten positioning grooves (8) are respectively positioned among the ten conductors (4); the centers of the ten conductors (4) are on the same circle, and the distances and the angles between two adjacent conductors (4) are the same; the conductors (4) are electrically connected in series.
5. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: ten clamping mechanisms (5) are arranged, have the same specification and are uniformly distributed by taking the test tube ring (3) as a reference; the ten clamping mechanisms (5) correspond to the ten positioning grooves (8), and the middle points of the clamping mechanisms (5) are positioned on the center lines of the positioning grooves (8).
6. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: the positioning rings (51) in the same group comprise two clamping ring structures which are symmetrically distributed and have the same specification, the middle parts of the clamping rings are annular, and the inner sides of the clamping rings are made of rubber materials with larger friction coefficients; the same group of the limiting columns (52) are provided with two limiting columns which respectively correspond to the same group of two positioning rings (51), the two limiting columns are respectively fixedly connected to one side, opposite to the two positioning rings (51), of the limiting columns, and the central lines of the two limiting columns and the central lines of the two positioning rings are the same straight line.
7. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: the driving shaft (71) and the central shaft (72) are in a concentric state, the frame bodies (73), the return springs (74) and the fixture blocks (75) are four and respectively correspond to each other, the specification sizes of the four frame bodies (73), the return springs (74) and the fixture blocks (75) are the same, the corresponding frame bodies (73), the return springs (74) and the fixture blocks (75) are in a group, four groups are arranged, and the four groups are uniformly distributed by taking the central shaft (72) as a reference.
8. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: the four electromagnetic devices (76) are arranged and have the same specification, and the four electromagnetic devices (76) are respectively and electrically connected with the coil (1) and the conductor (4); the fixture blocks (75) are made of magnetic materials, magnetic poles of the fixture blocks are N poles, four clamping grooves are formed in the inner side of the inner mounting plate (6), and the four clamping grooves are identical in specification and size and correspond to the four fixture blocks (75) respectively.
9. The test tube rack for biological cell research and the application thereof according to claim 1 are characterized in that: the four electromagnetic devices (76) respectively correspond to the four fixture blocks (75), the electromagnetic devices (76) generate magnetism under rated current intensity, and magnetic poles are S poles; the distances between the four electromagnetic devices (76) and the corresponding fixture blocks (75) are the same, and the centers of the four electromagnetic devices (76) are on the same circle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010884373.5A CN111940005A (en) | 2020-08-28 | 2020-08-28 | Test tube rack for biological cell research and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010884373.5A CN111940005A (en) | 2020-08-28 | 2020-08-28 | Test tube rack for biological cell research and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111940005A true CN111940005A (en) | 2020-11-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010884373.5A Withdrawn CN111940005A (en) | 2020-08-28 | 2020-08-28 | Test tube rack for biological cell research and application thereof |
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| Country | Link |
|---|---|
| CN (1) | CN111940005A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113856785A (en) * | 2021-10-25 | 2021-12-31 | 马邓友 | Medical sampling bottle storage rack easy to quickly find and provided with verification structure |
-
2020
- 2020-08-28 CN CN202010884373.5A patent/CN111940005A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113856785A (en) * | 2021-10-25 | 2021-12-31 | 马邓友 | Medical sampling bottle storage rack easy to quickly find and provided with verification structure |
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Application publication date: 20201117 |