CN111925534A - Preparation method of polyacrylamide gel capable of being rapidly and uniformly solidified - Google Patents
Preparation method of polyacrylamide gel capable of being rapidly and uniformly solidified Download PDFInfo
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- CN111925534A CN111925534A CN202010770277.8A CN202010770277A CN111925534A CN 111925534 A CN111925534 A CN 111925534A CN 202010770277 A CN202010770277 A CN 202010770277A CN 111925534 A CN111925534 A CN 111925534A
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- polyacrylamide gel
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- agarose solution
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/005—Processes for mixing polymers
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/12—Agar-agar; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2333/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
- C08J2333/24—Homopolymers or copolymers of amides or imides
- C08J2333/26—Homopolymers or copolymers of acrylamide or methacrylamide
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/12—Agar-agar; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2433/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
- C08J2433/24—Homopolymers or copolymers of amides or imides
- C08J2433/26—Homopolymers or copolymers of acrylamide or methacrylamide
Abstract
The application discloses a preparation method of quickly and uniformly solidified polyacrylamide gel, which comprises the following steps of selecting two pieces of glass with the same size and agarose solution; step two, vertically placing the two pieces of glass in the step one in parallel; step three, injecting an agarose solution from the bottom of a cavity formed by the two pieces of glass until the bottoms of the two pieces of glass are completely sealed; and step four, injecting the polyacrylamide gel raw material into a cavity formed by the two pieces of glass. According to the invention, ultrasonic waves are emitted from the bottom end of the first side of the polyacrylamide gel to the top end of the second side of the polyacrylamide gel, so that the uniform distribution of the polyacrylamide gel can be rapidly realized; the method comprises the steps of heating agar to 60-70 ℃, filtering at the temperature higher than 60 ℃, recovering supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution to quickly solidify polyacrylamide gel.
Description
Technical Field
The application relates to the field of physics or chemistry, in particular to a preparation method of polyacrylamide gel capable of being rapidly and uniformly solidified.
Background
Polyacrylamide gel electrophoresis (PAGE) is the most basic method used in molecular biology research to perform qualitative and quantitative analysis on small amounts of proteins or nucleic acids. Sodium Dodecyl Sulfate (SDS) is added to the gel, namely SDS-PAGE, and Native PAGE is carried out without adding SDS.
At present, the polyacrylamide gel has a slow solidification speed, and the use is seriously influenced.
Disclosure of Invention
The application mainly aims to provide a preparation method of polyacrylamide gel capable of being solidified quickly and uniformly, so as to solve the problem that the polyacrylamide gel in the prior art is low in solidification speed and seriously affects use.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions:
according to a first aspect of embodiments of the present invention, there is provided a method for preparing a polyacrylamide gel that solidifies rapidly and uniformly, comprising the steps of:
selecting two pieces of glass with the same size and an agarose solution for later use;
step two, vertically placing the two pieces of glass in the step one in parallel;
step three, injecting an agarose solution from the bottom of a cavity formed by the two pieces of glass until the bottoms of the two pieces of glass are completely sealed;
step four, after the agarose solution in the step three is completely solidified, injecting the polyacrylamide gel raw material into a cavity formed by the two pieces of glass.
Further, the preparation method of the agarose solution comprises the steps of heating agar to 60-70 ℃, filtering at the temperature higher than 60 ℃, recovering supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution.
Further, the agar comprises any one or a combination of more than two of strip gracilaria agar, powder gracilaria agar and strip laver agar.
Further, the fourth step includes injecting the raw material of the polyacrylamide gel into a cavity formed by the two pieces of glass, emitting the first ultrasonic wave from the bottom end of the first side of the polyacrylamide gel to the top end of the second side of the polyacrylamide gel, and emitting the second ultrasonic wave from the bottom end of the second volume of the polyacrylamide gel to the top end of the first side of the polyacrylamide gel.
Further, the use gap of the first ultrasonic wave and the second ultrasonic wave is 0.6-0.8 s.
Further, the frequency of the first ultrasonic wave and the second ultrasonic wave is 15-25 khz.
Furthermore, before injecting the polyacrylamide gel raw material, the agarose solution is processed at constant temperature, the temperature is controlled at 40-50 ℃ and the time is 2-4 minutes.
Furthermore, the mass concentration of the agarose gel is 1.5-2%.
According to a first aspect of the embodiments of the present invention, there is provided a supporting device, which includes two equal-height platforms for placing glass sets and a buckle disposed on top of the equal-height platforms, the buckle has a groove for accommodating the glass sets, the buckles disposed on the equal-height platforms respectively clamp the glass therebetween, and a clamping block is disposed between the glass sets and the groove of the buckle.
Further, the glass group comprises two pieces of glass which are vertically placed in parallel.
The embodiment of the invention has the following advantages: the embodiment of the invention provides a preparation method of a polyacrylamide gel capable of being rapidly and uniformly solidified, wherein first ultrasonic waves are emitted from the bottom end of a first side of the polyacrylamide gel to the top end of a second side of the polyacrylamide gel, and second ultrasonic waves are emitted from the bottom end of a second volume of the polyacrylamide gel to the top end of the first side of the polyacrylamide gel, so that the polyacrylamide gel can be rapidly and uniformly distributed; the method comprises the steps of taking strip-shaped gracilaria agar, powdered gracilaria agar and strip-shaped laver agar as raw materials, heating the agar to 60-70 ℃, filtering at the temperature higher than 60 ℃, recovering supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution to quickly realize the solidification of polyacrylamide gel.
Drawings
Fig. 1 is a schematic structural diagram of a supporting device provided in embodiment 5 of the present invention;
the labels in the figure are: 1-front glass, 2-back glass, 3-equal height table and 4-clamping block.
Detailed Description
In order to make the technical solutions in the embodiments of the present application better understood, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to examples, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Example 1
The embodiment provides a preparation method of a polyacrylamide gel capable of being solidified quickly and uniformly, which comprises the following steps:
selecting two pieces of glass with the same size and an agarose solution for later use;
step two, vertically placing the two pieces of glass in the step one in parallel;
step three, injecting an agarose solution from the bottom of a cavity formed by the two pieces of glass until the bottoms of the two pieces of glass are completely sealed;
step four, after the agarose solution in the step three is completely solidified, injecting the polyacrylamide gel raw material into a cavity formed by the two pieces of glass.
Example 2
On the basis of the technical scheme of the embodiment 1, the preparation method of the agarose solution comprises the steps of heating the gracilaria agar to 60 ℃, then filtering the gracilaria agar at the temperature higher than 60 ℃, then recovering the supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution. Further, the fourth step includes injecting the raw material of the polyacrylamide gel into a cavity formed by the two pieces of glass, emitting the first ultrasonic wave from the bottom end of the first side of the polyacrylamide gel to the top end of the second side of the polyacrylamide gel, and emitting the second ultrasonic wave from the bottom end of the second volume of the polyacrylamide gel to the top end of the first side of the polyacrylamide gel. The usage gap between the first ultrasonic wave and the second ultrasonic wave was 0.6 s. The frequency of the first ultrasonic wave and the second ultrasonic wave is 15 khz. Before injecting the polyacrylamide gel raw material, the agarose solution is processed at constant temperature, the temperature is controlled at 40 ℃, and the time is 2 minutes. The mass concentration of the agarose gel is 1.5%.
Example 3
On the basis of the technical scheme of the embodiment 1, the preparation method of the agarose solution comprises the steps of heating the gracilaria agar to 70 ℃, then filtering the gracilaria agar at the temperature higher than 60 ℃, then recovering the supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution. Further, the fourth step includes injecting the raw material of the polyacrylamide gel into a cavity formed by the two pieces of glass, emitting the first ultrasonic wave from the bottom end of the first side of the polyacrylamide gel to the top end of the second side of the polyacrylamide gel, and emitting the second ultrasonic wave from the bottom end of the second volume of the polyacrylamide gel to the top end of the first side of the polyacrylamide gel. The usage gap between the first ultrasonic wave and the second ultrasonic wave was 0.8 s. The frequency of the first ultrasonic wave and the second ultrasonic wave is 25 khz. Before injecting the polyacrylamide gel raw material, the agarose solution is processed at constant temperature, the temperature is controlled at 50 ℃ and the time is 4 minutes. The mass concentration of the agarose gel is 2%.
Example 4
On the basis of the technical scheme of the embodiment 1, the preparation method of the agarose solution comprises the steps of heating the gracilaria agar to 65 ℃, then filtering the gracilaria agar at the temperature higher than 60 ℃, then recovering the supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution. Further, the fourth step includes injecting the raw material of the polyacrylamide gel into a cavity formed by the two pieces of glass, emitting the first ultrasonic wave from the bottom end of the first side of the polyacrylamide gel to the top end of the second side of the polyacrylamide gel, and emitting the second ultrasonic wave from the bottom end of the second volume of the polyacrylamide gel to the top end of the first side of the polyacrylamide gel. The usage gap between the first ultrasonic wave and the second ultrasonic wave was 0.7 s. The frequency of the first ultrasonic wave and the second ultrasonic wave is 20 khz. Before injecting the polyacrylamide gel raw material, the agarose solution is processed at constant temperature, the temperature is controlled at 45 ℃ and the time is 3 minutes. The mass concentration of the agarose gel is 1.7%.
Example 5
The embodiment provides a supporting device, including being used for placing two equal altitude platforms 3 of glass group and setting up the buckle at equal altitude platform 3 tops, the buckle has a recess that can hold glass group, sets up respectively at equal altitude platform 3 on with glass centre gripping in the middle, and is equipped with fixture block 4 between the recess of glass group and buckle. The glass group comprises a front glass 1 and a rear glass 2 which are vertically placed in parallel.
The principle of the application lies in that: the first ultrasonic wave is emitted from the bottom end of the first side of the polyacrylamide gel to the top end of the second side of the polyacrylamide gel, and the second ultrasonic wave is emitted from the bottom end of the second volume of the polyacrylamide gel to the top end of the first side of the polyacrylamide gel, so that the uniform distribution of the polyacrylamide gel can be quickly realized; the method comprises the steps of taking strip-shaped gracilaria agar, powdered gracilaria agar and strip-shaped laver agar as raw materials, heating the agar to 60-70 ℃, filtering at the temperature higher than 60 ℃, recovering supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution to quickly realize the solidification of polyacrylamide gel.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (10)
1. A preparation method of polyacrylamide gel capable of being solidified quickly and uniformly is characterized by comprising the following steps:
selecting two pieces of glass with the same size and an agarose solution for later use;
step two, vertically placing the two pieces of glass in the step one in parallel;
step three, injecting an agarose solution from the bottom of a cavity formed by the two pieces of glass until the bottoms of the two pieces of glass are completely sealed;
step four, after the agarose solution in the step three is completely solidified, injecting the polyacrylamide gel raw material into a cavity formed by the two pieces of glass.
2. The method for preparing a rapid and uniform solidification polyacrylamide gel according to claim 1, wherein: the preparation method of the agarose solution comprises the steps of heating agar to 60-70 ℃, then filtering at the temperature higher than 60 ℃, then recovering supernatant or filtrate, and separating and purifying the supernatant or the filtrate to obtain the required agarose solution.
3. The method for preparing the rapid and uniform solidification polyacrylamide gel according to claim 2, wherein: the agar comprises one or more of Gracilaria verrucosa agar, Gracilaria verrucosa agar powder, and Porphyra tenera agar.
4. The method for preparing the rapid and uniform solidification polyacrylamide gel according to claim 2, wherein: and the fourth step comprises the steps of injecting a polyacrylamide gel raw material into a cavity formed by the two pieces of glass, emitting first ultrasonic waves from the bottom end of the first side of the polyacrylamide gel to the top end of the second side of the polyacrylamide gel, and emitting second ultrasonic waves from the bottom end of the second side of the polyacrylamide gel to the top end of the first side of the polyacrylamide gel.
5. The method for preparing a rapid and uniform solidification polyacrylamide gel according to claim 4, wherein: the using gap between the first ultrasonic wave and the second ultrasonic wave is 0.6-0.8 s.
6. The method for preparing a rapid and uniform solidification polyacrylamide gel according to claim 4, wherein: the frequency of the first ultrasonic wave and the second ultrasonic wave is 15-25 khz.
7. The method for preparing a rapid and uniform solidification polyacrylamide gel according to claim 4, wherein: before injecting the polyacrylamide gel raw material, carrying out constant temperature treatment on the agarose solution, controlling the temperature at 40-50 ℃ and the time at 2-4 minutes.
8. The method for preparing a rapid and uniform solidification polyacrylamide gel according to claim 1, wherein: the mass concentration of the agarose gel is 1.5-2%.
9. A support device for use according to any one of claims 1 to 8, wherein: the glass group clamping device comprises two equal-height platforms for placing glass groups and buckles arranged at the tops of the equal-height platforms, each buckle is provided with a groove capable of accommodating the glass group, the buckles arranged on the equal-height platforms respectively clamp the glass in the middle, and a clamping block is arranged between the glass group and the groove of the buckle.
10. The support device of claim 9, wherein: the glass group comprises two pieces of glass which are vertically placed in parallel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010770277.8A CN111925534A (en) | 2020-08-03 | 2020-08-03 | Preparation method of polyacrylamide gel capable of being rapidly and uniformly solidified |
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| CN202010770277.8A CN111925534A (en) | 2020-08-03 | 2020-08-03 | Preparation method of polyacrylamide gel capable of being rapidly and uniformly solidified |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104558322A (en) * | 2013-10-15 | 2015-04-29 | 中国石油化工股份有限公司 | Method for polymerizing acrylamide by ultrasonic technology |
| CN105263606A (en) * | 2013-03-15 | 2016-01-20 | 生物辐射实验室股份有限公司 | Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability |
| CN106568825A (en) * | 2016-11-11 | 2017-04-19 | 浙江农林大学 | Polyacrylamide gel preparation method capable of preventing solution leakage |
| CN108387630A (en) * | 2018-01-29 | 2018-08-10 | 温州科技职业学院 | A kind of improvement SDS- polyacrylamide gel electrophoresis techniques |
-
2020
- 2020-08-03 CN CN202010770277.8A patent/CN111925534A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105263606A (en) * | 2013-03-15 | 2016-01-20 | 生物辐射实验室股份有限公司 | Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability |
| CN104558322A (en) * | 2013-10-15 | 2015-04-29 | 中国石油化工股份有限公司 | Method for polymerizing acrylamide by ultrasonic technology |
| CN106568825A (en) * | 2016-11-11 | 2017-04-19 | 浙江农林大学 | Polyacrylamide gel preparation method capable of preventing solution leakage |
| CN108387630A (en) * | 2018-01-29 | 2018-08-10 | 温州科技职业学院 | A kind of improvement SDS- polyacrylamide gel electrophoresis techniques |
Non-Patent Citations (1)
| Title |
|---|
| 肖文静等: "超声促进聚合物合成研究进展", 《江苏化工》 * |
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Application publication date: 20201113 |