CN111921563A - Cobalt-based mimic enzyme and preparation method and application thereof - Google Patents
Cobalt-based mimic enzyme and preparation method and application thereof Download PDFInfo
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- 229910017052 cobalt Inorganic materials 0.000 title claims abstract description 97
- 239000010941 cobalt Substances 0.000 title claims abstract description 97
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 title claims abstract description 97
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 59
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 59
- 230000003278 mimic effect Effects 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 36
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims abstract description 21
- 235000018417 cysteine Nutrition 0.000 claims abstract description 21
- 108010024636 Glutathione Proteins 0.000 claims abstract description 18
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical group OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229960003180 glutathione Drugs 0.000 claims abstract description 18
- 239000002105 nanoparticle Substances 0.000 claims abstract description 16
- 235000018102 proteins Nutrition 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 15
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 15
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims abstract description 13
- 230000004048 modification Effects 0.000 claims abstract description 7
- 238000012986 modification Methods 0.000 claims abstract description 7
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 60
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 34
- 238000003756 stirring Methods 0.000 claims description 18
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 17
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 17
- 239000002245 particle Substances 0.000 claims description 12
- 239000007853 buffer solution Substances 0.000 claims description 10
- 238000000502 dialysis Methods 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 239000013078 crystal Substances 0.000 claims description 7
- 229910001429 cobalt ion Inorganic materials 0.000 claims description 6
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 claims description 6
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 5
- 230000035484 reaction time Effects 0.000 claims description 5
- 229910021503 Cobalt(II) hydroxide Inorganic materials 0.000 claims description 4
- 230000032683 aging Effects 0.000 claims description 4
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 3
- 108010067306 Fibronectins Proteins 0.000 claims description 3
- 102000016359 Fibronectins Human genes 0.000 claims description 3
- 108091006905 Human Serum Albumin Proteins 0.000 claims description 3
- 102000008100 Human Serum Albumin Human genes 0.000 claims description 3
- 108010058846 Ovalbumin Proteins 0.000 claims description 3
- 229940098773 bovine serum albumin Drugs 0.000 claims description 3
- UFMZWBIQTDUYBN-UHFFFAOYSA-N cobalt dinitrate Chemical compound [Co+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O UFMZWBIQTDUYBN-UHFFFAOYSA-N 0.000 claims description 3
- 229910001981 cobalt nitrate Inorganic materials 0.000 claims description 3
- 229940044175 cobalt sulfate Drugs 0.000 claims description 3
- 229910000361 cobalt sulfate Inorganic materials 0.000 claims description 3
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 claims description 3
- INPLXZPZQSLHBR-UHFFFAOYSA-N cobalt(2+);sulfide Chemical compound [S-2].[Co+2] INPLXZPZQSLHBR-UHFFFAOYSA-N 0.000 claims description 3
- ASKVAEGIVYSGNY-UHFFFAOYSA-L cobalt(ii) hydroxide Chemical compound [OH-].[OH-].[Co+2] ASKVAEGIVYSGNY-UHFFFAOYSA-L 0.000 claims description 3
- 229940092253 ovalbumin Drugs 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 102000008857 Ferritin Human genes 0.000 claims description 2
- 108050000784 Ferritin Proteins 0.000 claims description 2
- 238000008416 Ferritin Methods 0.000 claims description 2
- 229940011182 cobalt acetate Drugs 0.000 claims description 2
- QAHREYKOYSIQPH-UHFFFAOYSA-L cobalt(II) acetate Chemical compound [Co+2].CC([O-])=O.CC([O-])=O QAHREYKOYSIQPH-UHFFFAOYSA-L 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 238000002156 mixing Methods 0.000 description 12
- UBEWDCMIDFGDOO-UHFFFAOYSA-N cobalt(2+);cobalt(3+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[O-2].[Co+2].[Co+3].[Co+3] UBEWDCMIDFGDOO-UHFFFAOYSA-N 0.000 description 8
- 239000007974 sodium acetate buffer Substances 0.000 description 8
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 6
- 238000012512 characterization method Methods 0.000 description 6
- 238000002296 dynamic light scattering Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 230000002431 foraging effect Effects 0.000 description 5
- 238000003384 imaging method Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000006911 enzymatic reaction Methods 0.000 description 4
- 238000007792 addition Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 150000002978 peroxides Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000004627 transmission electron microscopy Methods 0.000 description 3
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000009088 enzymatic function Effects 0.000 description 1
- -1 ferroglobin Proteins 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 150000002500 ions Chemical group 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 229940031182 nanoparticles iron oxide Drugs 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
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- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
- B01J31/003—Catalysts comprising hydrides, coordination complexes or organic compounds containing enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
- B01J31/26—Catalysts comprising hydrides, coordination complexes or organic compounds containing in addition, inorganic metal compounds not provided for in groups B01J31/02 - B01J31/24
- B01J31/28—Catalysts comprising hydrides, coordination complexes or organic compounds containing in addition, inorganic metal compounds not provided for in groups B01J31/02 - B01J31/24 of the platinum group metals, iron group metals or copper
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- B01J35/00—Catalysts, in general, characterised by their form or physical properties
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Abstract
The invention provides a cobalt-based mimic enzyme and a preparation method and application thereof, wherein the cobalt-based mimic enzyme comprises cobalt-based nanoparticles and a biological modification molecule compounded outside the cobalt-based nanoparticles, and the biological modification molecule comprises any one or combination of at least two of a biological protein molecule, glutathione or cysteine molecule. The cobalt-based mimic enzyme has excellent mimic enzyme characteristics and can effectively catalyze the decomposition of hydrogen peroxide.
Description
Technical Field
The invention belongs to the technical field of biological materials, and relates to a cobalt-based mimic enzyme, and a preparation method and application thereof.
Background
The mimic enzyme is a substance with similar biological enzyme activity synthesized by a chemical method, and is mainly divided into organic high-molecular mimic enzyme and nano mimic enzyme (hereinafter referred to as nano enzyme) developed along with nano science and technology in recent years. The nature of the mimic enzyme is defined as that of a catalyst with enzyme property synthesized by an artificial method, and compared with biological enzyme, the mimic enzyme has the advantages of simple structure, stable chemical property, enzyme function, high efficiency, high selectivity, low price, easy obtainment and the like. The main structure of the nano material enzyme which is researched more is iron oxide nano particles, and the nano material enzyme achieves the effect similar to biological enzyme based on the valence-variable property of elements such as iron and the like, and is widely researched in the application of biological treatment.
Besides iron element, other transition metal elements also have valence-variable properties, such as copper, cobalt, manganese and other elements, and the non-iron-based nano-bio-enzyme is one of the hot spots of more researches in recent years in the field of nano-bio research.
CN104568934A discloses application of nano cobaltosic oxide as a peroxide mimic enzyme to determination of hydrogen peroxide, and the invention discovers that the nano cobaltosic oxide has the function of the peroxide mimic enzyme and can be used for detecting the content of the hydrogen peroxide; compared with HRP (horse radish peroxidase), the nano cobaltosic oxide prepared by the invention has high stability, can be repeatedly used and has high-efficiency catalytic activity of peroxide mimic enzyme; it is found that the nanometer cobaltosic oxide can also catalyze the color developing agent to be used as a mimic enzyme in the absence of hydrogen peroxide.
CN109650463A discloses a preparation method and application of a cobaltosic-peroxide mimic enzyme material. The material can be used as a novel mimic enzyme, has potential application value in the fields of immunoassay, biological detection, clinical diagnosis and the like, and has wide application prospect in novel catalytic oxidation analysis.
However, these cobalt mimetic enzyme materials disclosed above have poor biocompatibility. In the art, it is desirable to develop a cobalt-based mimic enzyme material that is biocompatible and has good mimic enzyme properties.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a cobalt-based mimic enzyme, and a preparation method and application thereof. The cobalt-based nanoenzyme has excellent enzyme simulation characteristics, can effectively catalyze the decomposition of hydrogen peroxide, has good biocompatibility, and has good application prospects in the fields of biological medicines and the like.
In order to achieve the purpose, the invention adopts the following technical scheme:
in one aspect, the invention provides a cobalt-based mimic enzyme, which comprises cobalt-based nanoparticles and a biological modification molecule compounded outside the cobalt-based nanoparticles, wherein the biological modification molecule comprises any one or a combination of at least two of a biological protein molecule, glutathione or cysteine molecule.
Preferably, the cobalt-based nanoparticles of the present invention are cobalt sulfide nanoparticles or cobalt hydroxide nanoparticles. The cobalt ions are combined with sulfur elements in cysteine or glutathione to form cobalt sulfide nanoparticles, and if the reaction substrate of the system has no cysteine or glutathione, the cobalt ions and hydroxide ions form cobalt hydroxide nanoparticles.
Preferably, the cobalt-based nanoparticles have a particle size of 0.5 to 20nm, such as 0.5nm, 1nm, 2nm, 3nm, 5nm, 8nm, 10nm, 12nm, 14nm, 16nm, 18nm, or 20 nm.
Preferably, the molar ratio of the biological protein molecules to the cobalt element is 1 (10-200), such as 1:10, 1:15, 1:20, 1:25, 1:30, 1:40, 1:50, 1:60, 1:80, 1:100, 1:120, 1:140, 1:160, 1:180, 1:200, and the like.
Preferably, the molar ratio of the cobalt element to the glutathione or cysteine molecule in the cobalt-based mimic enzyme is 1 (0.05-1), such as 1:0.05, 1:0.08, 1:0.1, 1:0.3, 1:0.5, 1:0.8, 1:1, and the like.
Preferably, the biological protein molecule is any one of bovine serum albumin, human serum albumin, ferroglobin, fibronectin or ovalbumin or a combination of at least two of them.
The cobalt-based nanoenzyme constructed by the invention can effectively realize the activity of the biological enzyme, and hydrogen peroxide (hereinafter referred to as H)2O2) Has stronger catalytic ability.
In another aspect, the invention provides a method for preparing a cobalt-based mimic enzyme, wherein the method comprises the following steps:
(1) adding the solution containing cobalt into sodium bicarbonate buffer solution of biological protein molecules, and reacting under stirring;
(2) and (2) adding a glutathione solution or a cysteine solution into the reaction solution obtained in the step (1) to assist the formation of crystal grains, then continuously stirring and reacting at a constant temperature of 37 ℃, then standing and aging to obtain a cobalt-based nano enzyme solution, dialyzing, purifying and freeze-drying to obtain the cobalt-based mimic enzyme.
Preferably, the concentration of the biological protein molecules in the sodium bicarbonate buffer solution of the biological protein molecules in the step (1) is 5-50 g/L, such as 5g/L, 8g/L, 10g/L, 13g/L, 15g/L, 18g/L, 20g/L, 25g/L, 30g/L, 35g/L, 38g/L, 40g/L, 45g/L, 48g/L or 50 g/L.
Preferably, the pH of the sodium bicarbonate buffer of step (1) is 6-10, such as 6, 6.5, 6.8, 7, 7.4, 7.8, 8, 8.4, 8.7, 9, 9.5, 9.8 or 10, etc.
Preferably, the solution containing cobalt in step (1) is a solution containing cobalt ions, which is obtained by dissolving inorganic salt containing cobalt or cobalt acetate in water;
preferably, the inorganic salt containing cobalt element is any one of cobalt chloride, cobalt sulfate or cobalt nitrate or a combination of at least two of them.
Preferably, the concentration of cobalt ions in the cobalt element-containing solution in step (1) is 0.05-1 mol/L, such as 0.05mol/L, 0.06mol/L, 0.08mol/L, 0.1mol/L, 0.2mol/L, 0.3mol/L, 0.4mol/L, 0.5mol/L, 0.6mol/L, 0.7mol/L, 0.8mol/L, 0.9mol/L or 1 mol/L.
Preferably, the molar ratio of the biological protein molecule in the step (1) to the cobalt element in the solution containing the cobalt element is 1 (10-200), such as 1:10, 1:15, 1:20, 1:25, 1:30, 1:40, 1:50, 1:60, 1:80, 1:100, 1:120, 1:140, 1:160, 1:180, 1:200, and the like.
Preferably, the reaction time in step (1) is 1 to 10 minutes, such as 1 minute, 2 minutes, 3 minutes, 4 minutes, 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes or 10 minutes, preferably 5 minutes.
In the invention, the reaction time in the step (1) is controlled, so as to control the adding time of the solution of glutathione or cysteine in the step (2), namely the adding time of the solution of glutathione or cysteine is 1-10 minutes after the solution containing cobalt element is mixed and reacted with the solution of biological protein molecules, and the more preferable adding time of the solution of glutathione or cysteine is 5 minutes after the solution containing cobalt element is mixed and reacted with the solution of biological protein molecules.
Preferably, the concentration of the glutathione solution or the solution of cysteine of step (2) is 5-100mmol/L, such as 5mmol/L, 8mmol/L, 10mmol/L, 20mmol/L, 30mmol/L, 40mmol/L, 50mmol/L, 60mmol/L, 70mmol/L, 80mmol/L, 90mmol/L or 100 mmol/L.
Preferably, the molar ratio of the cobalt element in the solution containing cobalt element in step (1) to the glutathione or cysteine in step (2) is 1 (0.05-1), such as 1:0.05, 1:0.08, 1:0.1, 1:0.3, 1:0.5, 1:0.8, 1:1, etc.
Preferably, the rotation speed of the stirring in step (2) is 500 and 1200 revolutions per minute, such as 500 revolutions per minute, 600 revolutions per minute, 700 revolutions per minute, 800 revolutions per minute, 900 revolutions per minute, 1000 revolutions per minute, 1100 revolutions per minute or 1200 revolutions per minute.
Preferably, the stirring reaction time in step (2) is 1-10 hours, such as 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours or 10 hours.
Preferably, the standing and aging time in step (2) is 8-24 hours, such as 8 hours, 9 hours, 10 hours, 12 hours, 14 hours, 15 hours, 16 hours, 18 hours, 20 hours, 22 hours, 24 hours and the like.
Preferably, the dialysis in step (2) is performed by selecting a dialysis bag with a cut-off molecular weight of 3000-15000 Da (such as 3000Da, 5000Da, 7000Da, 8000Da, 10000Da, 12000Da, 14000Da or 15000Da) and dialyzing for 1-3 days.
In the invention, the constructed cobalt-based nanoenzyme is added into 450 microliters of acetic acid-sodium acetate buffer solutions with different pH values, after uniform mixing, 3',5,5' -tetramethylbenzidine (hereinafter referred to as TMB) is added and uniformly mixed by shaking, and the performance of the added cobalt-based nanoenzyme is tested.
The pH range of the acetic acid-sodium acetate buffer chosen at the time of the test was 2-12. The concentration of the cobalt-based nanoenzyme is preferably 5-100 grams per liter. The selected volume of TMB added is 2.5-40 microliters.
In addition, in the invention, the constructed cobalt-based nanoenzyme is added into 450 microliters of acetic acid-sodium acetate buffer solutions with different pH values, and after uniform mixing, TMB and H are added2O2And shaking and mixing uniformly, testing the existence of H in the added cobalt-based nanoenzyme2O2Simulated enzyme performance in the presence.
The pH range of the acetic acid-sodium acetate buffer chosen at the time of the test was 2-12. The concentration of the cobalt-based nanoenzyme is preferably 5-100 grams per liter. The concentration of TMB added is chosen to be 10-100 millimoles per liter and the volume is 2.5-40 microliters. Selected addition of H2O2The volume of the solution (30% aqueous solution) is 10-100. mu.l.
Through tests, the cobalt-based nanoenzyme has excellent simulated enzyme characteristics.
In another aspect, the invention provides a cobalt-based mimic enzyme as described above in catalyzing hydrogen peroxide (H)2O2) Application in decomposition.
Compared with the prior art, the invention has the following beneficial effects:
the cobalt-based mimic enzyme constructed by the invention can be effective under the condition of low pH value (especially in the environment with the pH value of 2-6.5), has excellent mimic enzyme characteristics, and can also effectively catalyze the decomposition of hydrogen peroxide.
Drawings
FIG. 1 is a graph showing the dynamic light scattering characterization of cobalt-based nanoenzymes prepared in example 1 of the present invention.
FIG. 2 is an electron transmission microscope image of the cobalt-based nanoenzyme prepared in example 1 of the present invention, with a scale of 50 nm.
FIG. 3 is a graph showing the results of the enzyme performance measurement of cobalt-based nanoenzymes in different pH environments obtained in example 3 of the present invention.
FIG. 4 is a graph showing the results of the enzyme performance measurement of H2O 2-based nanoenzymes in different pH environments obtained in example 4 of the present invention.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Example 1
Preparation of cobalt-based nanoenzyme
1) Bovine serum albumin (hereinafter referred to as BSA) was added to a sodium bicarbonate buffer solution of 0.1mol/L and pH 8 at a final concentration of 10g/L, 6 ml of a sodium bicarbonate solution of BSA was taken after completion of dissolution by stirring, transferred to a 20 ml reaction flask, followed by adding 0.6 ml of a cobalt chloride solution of 0.4mol/L and mixing and reacting for 5 minutes,
2) adding 0.3 ml of glutathione aqueous solution with the concentration of 0.1mol per liter into the solution obtained in the step 1) to assist the formation of crystal grains, then keeping on vigorously stirring for 4 hours at constant temperature of 37 ℃, then standing for 12 hours for aging, and then dialyzing for 3 days by using a dialysis bag with the molecular weight cutoff of 10000.
The cobalt-based nanoenzyme obtained by the preparation method is characterized by dynamic light scattering (instrument model MALVERN Zetasizer Nano ZS), and the result is shown in fig. 1, and as can be seen from fig. 1, the average hydrated particle size of the cobalt-based nanoenzyme prepared in example 1 of the invention is 13.69 nanometers.
The cobalt-based nanoenzyme obtained by the preparation is subjected to imaging characterization by an electron transmission microscope (instrument model number TECNAI G220-S-TWIN), and the result is shown in figure 2, and as can be seen from figure 2, the cobalt-based nanoenzyme prepared in example 1 of the invention has an average particle size of 1.2 nm.
Example 2
1) Adding ovalbumin into sodium bicarbonate buffer solution with pH of 7.5 and concentration of 20g/L finally, taking 6 ml of BSA sodium bicarbonate solution after stirring and dissolving completely, transferring the BSA sodium bicarbonate solution into a 20 ml reaction bottle, and adding 0.3 ml of cobalt chloride solution with concentration of 0.2mol/L for mixing and reacting for 5 minutes;
2) adding 0.6 ml of cysteine aqueous solution with the concentration of 0.05mol per liter into the solution obtained in the step 1) to assist the formation of crystal grains, then keeping on vigorously stirring for 6 hours at a constant temperature of 37 ℃, then standing for 8 hours for aging, and then dialyzing for 2 days by using a dialysis bag with the molecular weight cutoff of 8000.
The cobalt-based nanoenzyme prepared by the embodiment is characterized by dynamic light scattering, and the average hydrated particle size is 15.72 nanometers; the transmission electron microscopy imaging characterization shows that the cobalt-based nanoenzyme prepared in the example has an average particle size of 2.61 nanometers.
Example 3
1) Adding human serum albumin with the final concentration of 5g/L into sodium bicarbonate buffer solution with the pH value of 6 and 0.1mol/L, stirring and dissolving completely, taking 6 ml of BSA sodium bicarbonate solution, transferring into a 20 ml reaction bottle, adding 0.3 ml of cobalt sulfate solution with the concentration of 0.05mol/L, and mixing and reacting for 10 minutes;
2) adding 0.6 ml of cysteine aqueous solution with the concentration of 0.05mol per liter into the solution obtained in the step 1) to assist the formation of crystal grains, then keeping on vigorously stirring for 1 hour at a constant temperature of 37 ℃, then standing for 12 hours for aging, and then dialyzing for 1 day by using a dialysis bag with the molecular weight cutoff of 15000.
The cobalt-based nanoenzyme prepared by the embodiment is characterized by dynamic light scattering, and the average hydrated particle size is 13.19 nanometers; the transmission electron microscopy imaging characterization shows that the cobalt-based nanoenzyme prepared in the embodiment has an average particle size of 1.67 nm.
Example 4
1) Adding the final ferritin with the concentration of 30g per liter into a sodium bicarbonate buffer solution with the pH value of 10 and the concentration of 0.1mol per liter, stirring and dissolving completely, taking 6 ml of a sodium bicarbonate solution of BSA, transferring into a 20 ml reaction bottle, and adding 0.3 ml of a cobalt nitrate solution with the concentration of 1mol per liter for mixing and reacting for 1 minute;
2) adding 0.6 ml of cysteine aqueous solution with the concentration of 0.05mol per liter into the solution obtained in the step 1) to assist the formation of crystal grains, then keeping on vigorously stirring for 1 hour at a constant temperature of 37 ℃, then standing for 24 hours for aging, and then dialyzing for 3 days by using a dialysis bag with the molecular weight cutoff of 5000.
The cobalt-based nanoenzyme prepared by the embodiment is characterized by dynamic light scattering, and the average hydrated particle size is 10.59 nanometers; the imaging characterization of an electron transmission microscope shows that the average particle size of the cobalt-based nanoenzyme prepared in the embodiment is 0.92 nanometer.
Example 5
1) Adding fibronectin to 0.1mol/L sodium bicarbonate buffer solution with pH of 8 at a final concentration of 30g/L, stirring to dissolve completely, taking 6 ml of BSA sodium bicarbonate solution, transferring into a 20 ml reaction flask, adding 0.3 ml of cobalt chloride solution with a concentration of 1mol/L, and mixing for reaction for 5 minutes;
2) adding 0.6 ml of cysteine aqueous solution with the concentration of 0.05mol per liter into the solution obtained in the step 1) to assist the formation of crystal grains, then keeping on vigorously stirring for 5 hours at a constant temperature of 37 ℃, then standing for 12 hours for aging, and then dialyzing for 2 days by using a dialysis bag with the molecular weight cutoff of 8000.
The cobalt-based nanoenzyme prepared by the embodiment is characterized by dynamic light scattering, and the average hydrated particle size is 9.92 nanometers; the transmission electron microscopy imaging characterization shows that the cobalt-based nanoenzyme prepared in the embodiment has an average particle size of 1.69 nanometers.
Example 6
The method for investigating the simulated enzyme performance of the cobalt-based nanoenzyme comprises the following steps:
adding the constructed cobalt-based nanoenzyme into 450 microliters of acetic acid-sodium acetate buffer solution with different pH values to enable the final concentration to be 25 grams per liter, uniformly mixing, adding 10 microliters of TMB solution with the concentration of 40 millimoles per liter, uniformly mixing by shaking, and testing the performance of the added cobalt-based nanoenzyme.
The pH range of the selected acetic acid-sodium acetate buffer is 2-12.
As shown in FIG. 3, FIG. 3 shows the results of activity detection of cobalt-based nanoenzyme in different pH environments, and it is found that the solution in acidic solution has a color change phenomenon, the solution at pH 3-5 has a significant color change, and especially the solution at pH 4 has a darker blue color. The cobalt-based nanoenzyme has excellent enzyme simulation performance in a low pH value environment, and particularly has an obvious enzymatic reaction in an environment with pH of 3-5, and the enzymatic reaction is more excellent when the pH is 4.
Example 7
H2O2The method for measuring the simulated enzyme performance of the cobalt-based nanoenzyme in the presence of the cobalt-based nanoenzyme comprises the following steps:
adding the constructed cobalt-based nanoenzyme into 450 microliters of acetic acid-sodium acetate buffer solution with different pH values to enable the final concentration to be 25 grams per liter, and after uniform mixing, adding 10 microliters of TMB with the concentration of 40 millimoles per liter and H with the concentration of 30 percent2O2And shaking and mixing uniformly, and testing the performance of the added cobalt-based nanoenzyme mimic enzyme.
The pH range of the selected acetic acid-sodium acetate buffer is 2-12.
The results are shown in FIG. 4, in which FIG. 4 shows the addition of H to different pH environments2O2The simulated enzyme performance of the cobalt-based nanoenzyme is found out that the solution has color change in neutral and acid environments, particularly the color change in the acid environment is more obvious, and the solution with the pH value of 4 still has the darkest color, which indicates that the solution has the H color2O2Under the existing conditions, the simulated enzyme performance of the cobalt-based nanoenzyme is remarkably improved in a low pH value environment, particularly in an environment with the pH value of 2-6.5, the enzymatic reaction is obvious, and the enzymatic reaction is more excellent when the pH value is 4.
The applicant states that the cobalt-based mimic enzyme of the present invention and the preparation method and application thereof are illustrated by the above examples, but the present invention is not limited to the above examples, i.e., it is not meant that the present invention must be implemented by the above examples. It will be apparent to those skilled in the art that any modification of the present invention, equivalent substitutions of selected materials and additions of auxiliary components, selection of specific modes and the like, which are within the scope and disclosure of the present invention, are contemplated by the present invention.
Claims (10)
1. A cobalt-based mimic enzyme, which comprises cobalt-based nanoparticles and a biological modification molecule complexed outside the cobalt-based nanoparticles, wherein the biological modification molecule comprises any one of a biological protein molecule, glutathione or cysteine molecule, or a combination of at least two of the biological protein molecule, the glutathione or cysteine molecule.
2. The cobalt-based mimetic enzyme according to claim 1, wherein the cobalt-based nanoparticle is a cobalt sulfide nanoparticle or a cobalt hydroxide nanoparticle;
preferably, the cobalt-based nanoparticles have a particle size of 0.5 to 20 nm.
3. The cobalt-based mimic enzyme according to claim 1 or 2, wherein the molar ratio of the bioprotein molecule to cobalt element is 1 (10-200);
preferably, the molar ratio of the cobalt element to the glutathione or cysteine molecules in the cobalt-based mimic enzyme is 1 (0.05-1).
4. The cobalt-based mimetic enzyme according to any one of claims 1 to 3, wherein the biological protein molecule is any one of or a combination of at least two of bovine serum albumin, human serum albumin, ferritin, fibronectin, and ovalbumin.
5. The method for preparing a cobalt-based mimic enzyme according to any one of claims 1 to 4, wherein the method comprises the following steps:
(1) adding the solution containing cobalt into sodium bicarbonate buffer solution of biological protein molecules, and reacting under stirring;
(2) and (2) adding a glutathione solution or a cysteine solution into the reaction solution obtained in the step (1) to assist the formation of crystal grains, then continuously stirring and reacting at a constant temperature of 37 ℃, then standing and aging to obtain a cobalt-based nano enzyme solution, dialyzing, purifying and freeze-drying to obtain the cobalt-based mimic enzyme.
6. The preparation method according to claim 5, wherein the concentration of the bioprotein molecules in the sodium bicarbonate buffer solution of the bioprotein molecules in the step (1) is 5-50 g/L;
preferably, the pH value of the sodium bicarbonate buffer solution in the step (1) is 6-10.
7. The production method according to claim 5 or 6, wherein the solution containing cobalt in step (1) is a solution containing cobalt ions obtained by dissolving an inorganic salt containing cobalt or cobalt acetate in water;
preferably, the inorganic salt containing cobalt element is any one of cobalt chloride, cobalt sulfate or cobalt nitrate or a combination of at least two of them.
8. The method according to any one of claims 5 to 7, wherein the concentration of cobalt ions in the cobalt-containing solution of step (1) is 0.05 to 1 mol/L;
preferably, the molar ratio of the biological protein molecules in the step (1) to the cobalt element in the solution containing the cobalt element is 1 (10-200);
preferably, the reaction time in the step (1) is 1-10 minutes.
9. The production method according to any one of claims 5 to 8, wherein the concentration of the glutathione solution or the solution of cysteine in step (2) is 5 to 100 mmol/L;
preferably, the molar ratio of the cobalt element in the solution containing the cobalt element in the step (1) to the glutathione or cysteine in the step (2) is 1 (0.05-1);
preferably, the rotation speed of the stirring in the step (2) is 500-1200 rpm;
preferably, the stirring reaction time in the step (2) is 1-10 hours;
preferably, the standing and aging time of the step (2) is 8 to 24 hours;
preferably, the dialysis in the step (2) selects a dialysis bag with the cut-off molecular weight of 3000-15000 Da, and the dialysis is carried out for 1-3 days.
10. Use of a cobalt-based mimetic enzyme according to any one of claims 1 to 4 for catalyzing the decomposition of hydrogen peroxide.
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