CN111913295B - Three-color LED array illumination microscope - Google Patents
Three-color LED array illumination microscope Download PDFInfo
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- CN111913295B CN111913295B CN202010906587.8A CN202010906587A CN111913295B CN 111913295 B CN111913295 B CN 111913295B CN 202010906587 A CN202010906587 A CN 202010906587A CN 111913295 B CN111913295 B CN 111913295B
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- 238000005286 illumination Methods 0.000 title claims abstract description 65
- 230000000694 effects Effects 0.000 claims abstract description 9
- 230000003287 optical effect Effects 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 4
- 230000002708 enhancing effect Effects 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 2
- 230000000007 visual effect Effects 0.000 claims description 2
- 238000009827 uniform distribution Methods 0.000 claims 1
- 238000009434 installation Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 238000009826 distribution Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 4
- 238000003491 array Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000005282 brightening Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
Abstract
The invention discloses a three-color LED array illumination microscope, which comprises an LED array illumination device arranged on a microscope base and an aperture fixed at the bottom of an objective table and used for selecting an illumination area of an LED array light source, and is characterized in that the LED array illumination device comprises an LED array area light source, an LED brightness adjusting knob, an adjustable support, a power jack, a level V, a level H and an aperture; the LED array lighting device is formed by arranging screened LEDs according to a design scheme, and brightness is adjusted through an LED brightness adjusting knob; the adjustable support consists of three same cylindrical height-adjustable supports and is used for setting the height and the direction; the level gauge V and the level gauge H are mutually perpendicular and fixed on the surface light source, and are used for assisting the requirement of horizontal adjustment of the plane of the light source; and the aperture is fixed at the bottom of the objective table and is parallel to the axis of the light passing hole of the objective table. The device has the advantages of uniform brightness, high brightness, outstanding color effect, theoretical analysis uniformity of more than 90%, small volume, long service life and convenient installation when being projected to an observation area.
Description
Technical Field
The invention relates to a three-color LED array illumination microscope, in particular to a three-color LED array back illumination microscope.
Background
The earliest microscope was manufactured in the netherlands at the end of the 16 th century, and microscope technology developed over several hundred years and was widely and commonly used in the fields of medicine, biology, etc.
The method is divided into transmission type illumination and reflection type illumination according to a microscope illumination mode, wherein the transmission type illumination is suitable for observing transparent or semitransparent observation samples, and the reflection type illumination is suitable for observing non-transparent observation samples. The method comprises the following steps: back lighting, forward lighting, structured light, and stroboscopic lighting, etc. The back lighting is that the object to be measured is placed between the light source and the observation point, and the advantage is that high-contrast images can be obtained. In order to highlight information showing a certain color, a color matching combination is usually selected appropriately, and the same-tone light can lighten (lighten) the illuminated part and darken (darken) the illuminated part by using the complementary tone, thereby achieving the effect of enhancing the contrast.
The LED is a solid semiconductor light source, mainly comprising PN junction chips, electrodes and an optical system, is a four-generation illumination light source, and has the unique advantages of high electro-optic conversion efficiency, long service life, single luminous wavelength, good monochromaticity, pure light color, small attenuation, abundant wave bands, small heating value, small volume and light weight since the LED in the 60 th century is developed, and has obvious light and electric characteristics and spectrum distribution advantages compared with the traditional light source, and is concerned by people and widely applied. With the rapid development of science and technology and manufacturing process, the luminous brightness of the LED is improved by 20 times each year on average, the price is reduced to 1/100 of that of the first, and the LED has been widely and universally applied to the fields of daily lighting and a plurality of related disciplines.
Disclosure of Invention
The invention aims to solve the technical problem of providing an LED array illumination microscope which is uniform in distribution, small in size, high in electro-optic conversion efficiency and high in brightness and a design method of the illumination system.
The technical scheme adopted by the invention is as follows: the utility model provides a three-colour LED array illumination microscope, including LED array lighting device and light ring, LED array light source optical axis, microscope main optical axis, the three-wire parallel of objective table light-passing hole axis, LED array light source is placed on the microscope base, height and direction through adjustable support regulation LED array light source, the light ring is fixed in the objective table bottom and light ring axis and two-wire parallel of objective table light-passing hole axis, if two main axis coincidence effects are better, the light ring is used for screening illumination region and shelters from the inhomogeneous and unnecessary illumination in edge, the light that LED array light source projected out, through the screening of space stack and light ring, and the regulation of adjustable support, shine the objective table observation face and obtain illumination intensity distribution even region. In order to highlight information showing a certain color, a color matching combination is usually selected appropriately, and the same-tone light can lighten (lighten) the illuminated part and darken (darken) the illuminated part by using the complementary tone, thereby achieving the effect of enhancing the contrast.
The method for designing the LED array light source comprises the following specific steps:
the LED light source is selected, and three types of LEDs adopted by the invention form a group. Firstly, determining the color type of an LED, if a black solid circle selects a red LED, a black empty circle selects a yellow LED, and a gray solid circle selects a blue LEDThe method comprises the steps of carrying out a first treatment on the surface of the The LED group types can be switched, such as blue LEDs are selected from black solid circles, red LEDs are selected from black empty circles, yellow LEDs are selected from gray solid circles, and the like; secondly, the three types of the selected LEDs have the same visual angle theta so as to ensure that the value of m is the same when the illumination intensity is calculated, and the parameter m is defined byDetermining θ 1/2 The viewing angle, defined as half the value of 0 for illumination intensity, is called half the illumination intensity angle, and the three types of LEDs have the same optimal pitch when designing the LED array, all LED groups remain parallel.
And establishing a rectangular coordinate system plane X0Y by taking the center of the plane where the LED array light source is positioned as an origin 0, taking a perpendicular line of the plane X0Y passing through the origin 0 as a Z axis, taking the intersection point of the plane X0Y and the Z axis as 0, and taking the positive direction of the Z axis from the mirror base to the object stage. In the plane X0Y, a group of parallel lines parallel to the X axis are formed by an interval d, a group of parallel lines parallel to the Y axis are formed by an interval d, and the intersection points are LED group arrangement points, so that an N multiplied by N LED array is obtained.
The observation surface is set as the plane of the objective table, and the distance between the observation surface and the plane of the LED array light source is recorded as z. The single LED is used as a lambertian body light source, and the illumination intensity is expressed as follows: i (θ) =i0·cos m (θ), where I 0 Representing the illumination intensity on the optical axis, θ is the viewing angle, and the illumination intensity at each point (x, y, 0) in the illumination reference plane is expressed as:
according to the separo rule and the superposition principle, the viewing-plane intensity can be expressed as follows, when N is even,
when N is an odd number, the number of the N,
wherein E is R 、E G 、E B The light intensity of the light emitted by the three primary LEDs is expressed in the same expression form, and the specific form is E t Shown, then the total illumination intensity e=e R +E G +E B N represents the number of rows and columns of the LED array, d represents the center-to-center distance between two nearest adjacent LEDs of the same type in the LED array, and N represents the number of rows and columns of the LEDs of the LED array light source. From mathematical knowledge, when the total illumination intensity is evenly distributed, the best d satisfies the relationship:
compared with the traditional microscope at present, the invention has the advantages that the illumination distribution uniformity, the brightness and the color of the illumination component have the effect on the observation effect of the microscope, the maximum brightness which can be achieved by the illumination of the microscope is greatly improved after the high-brightness LED is adopted, and the requirement of the observation of the microscope on the illumination intensity is completely met. Based on the advantages of LEDs, the LED array lighting system has the advantages of small heating value, small volume, long service life, uniform illumination intensity, high maximum brightness value, pure light color, small attenuation and energy conservation, and achieves the brightening or darkening of the observation effect through LED type combination.
Drawings
FIG. 1 is an overall side elevational view of the present invention;
FIG. 2 is a side view of an LED array light source and aperture according to the present invention;
fig. 3 is a top view of the LED array light source of the present invention.
FIG. 4 is a diagram of an even row and column layout and an XY coordinate system of an LED array according to the present invention;
FIG. 5 is a diagram of an odd-numbered row-column layout and an XY coordinate system of an LED array according to the present invention;
Detailed Description
The following detailed description of implementations of the invention is provided in connection with the accompanying drawings.
The utility model provides a three-colour LED array illumination microscope, including LED array lighting device and light ring, LED array light source optical axis, microscope main optical axis, the three-wire parallel of objective table light-passing hole axis, LED array light source is placed on the microscope base, height and the direction through adjustable support regulation LED array light source, the light ring is fixed in the objective table bottom and two main shafts of light ring and objective table light-passing hole are parallel, if two main shafts coincide the effect better, the light ring is used for screening illumination region and shelters from the inhomogeneous illumination of edge and unnecessary illumination, the light that LED array light source projected out, through the screening of space stack and light ring, and the regulation of adjustable support, shine the objective table observation face and obtain illumination intensity distribution even region.
In accordance with the method of the present invention, a specific design of an 8 x 8LED array illumination source is performed as follows. And establishing a rectangular coordinate plane X0Y by taking the center of the plane where the LED array light source is positioned as an origin 0, taking a perpendicular line of the plane X0Y passing through the origin 0 as a Z axis, taking the intersection point of the plane X0Y and the Z axis as 0, and taking the direction from the mirror base to the object stage as the positive direction of the Z axis. In the plane X0Y, a group of 8 parallel lines parallel to the X axis are formed by an interval d, a group of 8 parallel lines parallel to the Y axis are formed by an interval d, and the intersection point is the arrangement point of the LEDs of the LED array, as shown in FIG. 4, and all LED subgroups are kept parallel.
Selecting a common LED, selecting a red LED from a black solid circle, selecting a yellow LED from a black empty circle, selecting a blue LED from a gray solid circle, and basically carrying out the following information on parameters of the LED: the standard working current of the red LED is 10mA, the standard working current of the blue LED is 20mA, the standard working current of the yellow LED is 10mA, and the half angle value theta 1/2 80.67 is calculated by corresponding to an m value of 7.5 degrees, wherein the m value is 81, the illumination maximum value of a red LED is 10cd, the illumination maximum value of a blue LED is 4cd, the illumination maximum value of a yellow LED is 8cd, and the distance from an LED array light source to an object stage observation surface, namely z, is 10cm.
Substituting the selected LED parameter basic information into the relation d of the optimal center distance of the LEDD=1.3 cm is calculated, specific position parameters of the arrangement of the LED arrays are determined, and the corresponding size of the arranged LED arrays is 9 multiplied by 9cm 2 Square matrix, the size of the illumination area of the LED array at z=10cm is 10×10cm 2 Via even number LED arrayThe column illumination intensity calculation formula is used for calculating, and the maximum value of the illumination intensity of the observation plane, which is 10cm away from the LED array, of the designed optimal distance is 515cd. The LED arrays with various colors are controlled by independent switches and adjusting knobs respectively.
The aperture is fixed at the bottom of the objective table, the LED array light source is placed on the lens base, the appropriate size of the aperture is adjusted, and the height and the direction of the light source are adjusted by adjusting the adjustable support.
Claims (6)
1. The three-color LED array illumination microscope comprises an LED array illumination device which is projected to an observation surface of an objective table and the optical axis of which is parallel to the main optical axis of the microscope, and an aperture which is fixed at the bottom of the objective table and is used for selecting an area where an LED array light source is projected uniformly, and is characterized in that the LED array illumination device comprises an LED array area light source, an LED brightness adjusting knob, an adjustable support, a power jack, a level meter and an aperture; the aperture is fixed at the bottom of the objective table and is parallel to the axis of the light passing hole of the objective table, the LED array lighting device is arranged on the lens seat and below the objective table, the optical axis of the LED array, the axis of the aperture and the axis of the light passing hole of the objective table are parallel to each other in three lines, the LED array lighting device consists of LEDs in the LED array, light generated by the LED array lighting device is overlapped by light emitted by each LED in the LED array, the LED array lighting device projects to the observation surface of the objective table to obtain an illumination area with uniform illumination intensity, two color LEDs in three primary colors are arranged at each LED placement point of the LED array lighting device, the LED placement points are arranged according to rows and columns, the LED array lighting device can select light source color combination matching, the matching color is identical to the observation target, the same color part of the observation target becomes bright, or the matching color and the color of the observation target becomes the complementary color relation, and the complementary color part of the observation target becomes dark, so that the effect of enhancing the contrast is achieved.
2. The three-color LED array illumination microscope of claim 1, wherein if two vertical symmetry axes of the LED array are respectively an X-axis and a Y-axis, the LED array optical axis is taken as a Z-axis direction, the direction of the pointing stage is taken as a positive direction, the intersection point of the three axes is taken as an origin of coordinates, and the origin is x=x0, Y=0, Z=0, set up a three-dimensional space coordinate system, if regard interval d as X axis parallel line in X0Y plane, regard interval d as Y axis parallel line, set up LED minor group in every crossing point, form LED array; the LED light sources are selected, and three types of LED light sources are adopted, wherein the three types of LED light sources comprise a red LED, a yellow LED and a blue LED, and each LED light source forms a group; the three types of LEDs are selected to have the same visual angle theta so as to ensure that m has the same value when calculating illumination intensity, and the three types of LEDs have the same optimal spacing d when designing the LED array, wherein the parameter m is defined byDetermining θ 1/2 An angle value defined as half the viewing angle value.
3. The three-color LED array illumination microscope of claim 1, wherein the light emitted from the LED array illumination device is projected from the lens base to the observation surface through the bottom of the stage, and the illumination intensity projected to the observation surface is uniformly distributed by selecting a desired illumination area when passing through the aperture at the bottom of the stage.
4. The three-color LED array illumination microscope of claim 1, wherein the LED array illumination device adjusts the height and the light source plane direction through the bottom adjustable support, and ensures uniform distribution of illumination intensity of the LED array light source projected on the observation surface through adjustment.
5. A three-color LED array illumination microscope as claimed in claim 1, wherein the LEDs of each type of LED of the LED array illumination device are independently adjustable, and each color LED is set according to the observed target color information.
6. A three-color LED array illumination microscope as claimed in claim 1, wherein the LED illumination intensity of the LED array illumination device is set according to the microscope usage requirement.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010906587.8A CN111913295B (en) | 2020-08-23 | 2020-08-23 | Three-color LED array illumination microscope |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010853415.9A CN111856738A (en) | 2020-08-23 | 2020-08-23 | LED array lighting microscope |
| CN202010906587.8A CN111913295B (en) | 2020-08-23 | 2020-08-23 | Three-color LED array illumination microscope |
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| CN111913295B true CN111913295B (en) | 2023-12-22 |
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| CN202010906587.8A Active CN111913295B (en) | 2020-08-23 | 2020-08-23 | Three-color LED array illumination microscope |
| CN202010853415.9A Pending CN111856738A (en) | 2020-08-23 | 2020-08-23 | LED array lighting microscope |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105158887A (en) * | 2015-09-29 | 2015-12-16 | 南京理工大学 | Multi-mode microimaging method based on programmable LED array illumination |
| CN106772978A (en) * | 2016-12-30 | 2017-05-31 | 宁波永新光学股份有限公司 | A kind of illuminated light microscope of LED reflection |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070211460A1 (en) * | 2006-03-09 | 2007-09-13 | Ilya Ravkin | Multi-color LED light source for microscope illumination |
| US20090251751A1 (en) * | 2008-04-02 | 2009-10-08 | Kurt Kuhlmann | Optical Imaging System |
| DE102013204945B4 (en) * | 2013-03-20 | 2015-03-26 | Leica Microsystems (Schweiz) Ag | Microscope with transmitted-light illumination for critical illumination |
| CN205507208U (en) * | 2016-04-13 | 2016-08-24 | 麦克奥迪实业集团有限公司 | Microscope lighting system based on LED array |
| CN212515203U (en) * | 2020-08-23 | 2021-02-09 | 浙江农林大学 | LED array lighting microscope |
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- 2020-08-23 CN CN202010902494.8A patent/CN112014966B/en active Active
- 2020-08-23 CN CN202010906587.8A patent/CN111913295B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105158887A (en) * | 2015-09-29 | 2015-12-16 | 南京理工大学 | Multi-mode microimaging method based on programmable LED array illumination |
| CN106772978A (en) * | 2016-12-30 | 2017-05-31 | 宁波永新光学股份有限公司 | A kind of illuminated light microscope of LED reflection |
Non-Patent Citations (1)
| Title |
|---|
| 基于可编程LED阵列照明的透射体视显微镜;孔富城等;《光学学报》;20160510(第05期);全文 * |
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| CN111913295A (en) | 2020-11-10 |
| CN112014966B (en) | 2023-12-22 |
| CN112014966A (en) | 2020-12-01 |
| CN111856738A (en) | 2020-10-30 |
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