CN111893031B - Sampling device for microbial detection of semisolid sample - Google Patents

Sampling device for microbial detection of semisolid sample Download PDF

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Publication number
CN111893031B
CN111893031B CN202010805770.9A CN202010805770A CN111893031B CN 111893031 B CN111893031 B CN 111893031B CN 202010805770 A CN202010805770 A CN 202010805770A CN 111893031 B CN111893031 B CN 111893031B
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outer shell
inner shell
sliding block
plate
piston
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CN111893031A (en
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王文博
邬元娟
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Institute of Agricultural Quality Standards and Testing Technology of Shandong Academy of Agricultural Sciences
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Institute of Agricultural Quality Standards and Testing Technology of Shandong Academy of Agricultural Sciences
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Publication of CN111893031A publication Critical patent/CN111893031A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

Abstract

The invention discloses a sampling device for detecting microorganisms of semisolid samples, which comprises a supporting flat plate, an upper pressing plate and an extracting mechanism, wherein a first long hole is arranged on the supporting flat plate, a sliding block is arranged between the extracting mechanism and the first long hole, the extracting mechanism comprises an outer shell, an inner shell and a piston, the outer shell penetrates through the sliding block and is in sliding connection with the sliding block, a suction nozzle is arranged at the lower end of the inner shell, an opening is arranged at the lower end of the outer shell, the suction nozzle penetrates through the opening, the upper end of the inner shell is detachably connected with the upper end of the outer shell, the piston is positioned in the inner shell and is in sealing fit with the inner shell, a piston rod is arranged at the upper end of the piston, the upper end of the piston rod is connected with the upper pressing plate, when in use, the position of the extracting mechanism is firstly adjusted along the first long hole, so that semisolid samples at different positions are extracted, the outer shell is moved up and down, and the samples at different depths of the semisolid samples are extracted, the upper pressing plate is lifted upwards, so that synchronous sampling of a plurality of extracting mechanisms can be completed at one time, and time and labor are saved.

Description

Sampling device for microbial detection of semisolid sample
Technical Field
The invention relates to the technical field of microbial detection, in particular to a sampling device for microbial detection of a semisolid sample.
Background
Semi-solid formulations, typically suspensions, gels, creams, ointments, creams, and the like. In the traditional sense, the preparation of a semi-solid preparation sample is always more troublesome than that of a solid preparation and a liquid preparation, the problems of troublesome sample preparation, long time consumption, poor reproducibility and the like are troubled by many people, moreover, the semi-solid preparation sample is difficult to accurately sample due to overlarge viscosity, and the steps of uniformly dispersing, extracting, filtering and the like of the sample also influence the quality of an experimental result.
The sampling of current semi-solid sample is carried out the sample many times through the manual work in the different positions of sample, the different degree of depth, wastes time and energy, and the sampling precision is low.
Disclosure of Invention
The invention aims to solve the problems of time and labor waste and low sampling precision of the semi-solid sample, and provides a sampling device for detecting microorganisms in the semi-solid sample, which can finish sampling at different positions and different depths at one time, and is time-saving, labor-saving and high in sampling precision.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a sampling device for detecting microorganisms in semisolid samples comprises a supporting flat plate, an upper pressing plate and an extracting mechanism, wherein a first strip hole is formed in the supporting flat plate, a plurality of first strip holes are uniformly formed in the circumferential direction of the supporting flat plate and correspond to the extracting mechanism one by one, a sliding block is arranged between the extracting mechanism and the first strip hole, the sliding block is connected with the first strip hole in a sliding mode, the extracting mechanism comprises an outer shell, an inner shell and a piston, the outer shell penetrates through the sliding block and is connected with the sliding block in a sliding mode, the inner shell is located in the outer shell and is connected with the outer shell in a sliding mode, a suction nozzle is arranged at the lower end of the inner shell, an opening is formed in the lower end of the outer shell, the suction nozzle penetrates through the opening, the upper end of the inner shell is detachably connected with the upper end of the outer shell, the piston is located in the inner shell and is in sealing fit with the inner shell, a piston rod is arranged at the upper end of the piston, and a second strip hole is formed in the upper pressing plate, the upper end of the piston rod is connected with the second strip hole;
the support plate is provided with a guide pillar, the upper pressing plate is provided with a guide sleeve, the guide pillar is matched with the guide sleeve, the upper end of the guide pillar is provided with a limit nut, and the limit nut is in threaded connection with the guide pillar.
Furthermore, a threaded sleeve is arranged at the lower end of the sliding block and penetrates through the first strip hole, a positioning plate is arranged in the middle of the sliding block and is in contact with the upper end face of the supporting flat plate, and a first nut is arranged on the threaded sleeve.
Further, be equipped with the through-hole in the slider, the shell body passes the through-hole, and the slider upper end is equipped with the sleeve, is equipped with locking screw on the sleeve.
Further, a sealing ring is arranged between the suction nozzle and the inner wall of the opening.
Further, the shell body upper end is equipped with the spacing groove, and the interior casing upper end is equipped with the stopper, stopper and spacing groove cooperation and through pin joint.
Furthermore, a second nut is arranged at the upper end of the piston rod, the upper end of the piston rod penetrates through the second strip hole, and the second nut is matched with the upper end face of the upper pressing plate.
Furthermore, a handle is arranged in the middle of the upper pressure plate.
Furthermore, the lower end of the support flat plate is provided with a support leg.
The invention has the beneficial effects that:
1. the invention comprises a supporting flat plate, an upper pressing plate and an extracting mechanism, wherein a first long hole is arranged on the supporting flat plate, a sliding block is arranged between the extracting mechanism and the first long hole, the extracting mechanism comprises an outer shell, an inner shell and a piston, the outer shell penetrates through the sliding block and is in sliding connection with the sliding block, a suction nozzle is arranged at the lower end of the inner shell, an opening is arranged at the lower end of the outer shell, the suction nozzle penetrates through the opening, the upper end of the inner shell is detachably connected with the upper end of the outer shell, the piston is positioned in the inner shell and is in sealing fit with the inner shell, a piston rod is arranged at the upper end of the piston, the upper end of the piston rod is connected with the upper pressing plate, when in use, the position of the extracting mechanism is adjusted along the first long hole, so that semi-solid samples at different positions are extracted, the outer shell is moved up and down, the lower ends of different extracting mechanisms penetrate into the semi-solid samples to different depths, and sampling of the semi-solid samples is realized, and then the upper pressure plate is lifted upwards, the piston moves upwards, and a plurality of extraction mechanisms can be synchronously sampled at one time, so that time and labor are saved.
2. According to the invention, the guide pillar is arranged on the support flat plate, the guide sleeve is arranged on the upper pressure plate, the guide pillar is matched with the guide sleeve, the limiting nut is arranged at the upper end of the guide pillar and is in threaded connection with the guide pillar 21, the upper pressure plate can move up and down along the guide pillar, and the position of the limiting nut can be adjusted up and down, so that the upward pulling distance of the upper pressure plate is adjusted, a sample with a required volume is obtained, and the experimental precision is high.
3. According to the invention, the lower end of the sliding block is provided with the threaded sleeve, the threaded sleeve penetrates through the first strip hole, the middle position of the sliding block is provided with the positioning plate, the positioning plate is in contact with the upper end surface of the supporting flat plate, the first nut is arranged on the threaded sleeve, the sliding block can be moved along the axis of the first strip hole by loosening the first nut through fixing the sliding block by the first nut, the position of the extraction mechanism can be adjusted, semi-solid samples at different positions can be sampled, and the practicability is good.
4. According to the invention, the through hole is formed in the sliding block, the outer shell penetrates through the through hole, the sleeve is arranged at the upper end of the sliding block, the locking screw is arranged on the sleeve, the outer shell can move up and down in the through hole, and the locking screw is used for locking, so that the sampling of semi-solid samples at different depths can be realized, and the sampling precision can be improved.
5. According to the invention, the limiting groove is formed in the upper end of the outer shell, the limiting block is arranged at the upper end of the inner shell, the limiting block is matched with the limiting groove and connected with the inner shell through the pin, the inner shell can be taken out of the outer shell after sampling is finished, a sample can be conveniently taken out, meanwhile, the position between the outer shell and the supporting flat plate can be kept unchanged, next sampling can be carried out after cleaning, adjustment is not needed, and the practicability is good.
Drawings
In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a first schematic structural diagram of the present invention;
FIG. 2 is a second schematic structural view of the present invention;
fig. 3 is a drawing cross-sectional view of the present invention.
In the figure: the support plate comprises a support plate 1, an upper pressing plate 2, a first strip hole 3, a sliding block 4, an outer shell 5, an inner shell 6, a piston 7, a threaded sleeve 8, a positioning plate 9, a first nut 10, a sleeve 11, a locking screw 12, a suction nozzle 13, an opening 14, a piston rod 15, a limiting groove 16, a limiting block 17, a second strip hole 18, a second nut 19, a handle 20, a guide pillar 21, a guide sleeve 22, a limiting nut 23 and a supporting leg 24.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the drawings in the embodiment of the present invention, and it is obvious that the described embodiment is only a part of the embodiment of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
For convenience of description, a coordinate system is now defined as shown in fig. 1.
As shown in fig. 1 to 3, a sampling device for detecting microorganisms in a semisolid sample comprises a support plate 1, an upper pressing plate 2 and an extraction mechanism, wherein the upper pressing plate 2 is located above the support plate 1, the upper pressing plate 2 and the support plate 1 are both circular flat plates, the support plate 1 is provided with a plurality of first strip holes 3, the first strip holes 3 are uniformly arranged along the circumferential direction of the support plate 1, in this embodiment, six first strip holes 3 are provided, the first strip holes 3 correspond to the extraction mechanism one by one, a sliding block 4 is arranged between the extraction mechanism and the first strip holes 3, the sliding block 4 is connected with the first strip holes 3 in a sliding manner, the extraction mechanism comprises an outer shell 5, an inner shell 6 and a piston 7, the outer shell 5 penetrates through the sliding block 4 and is connected with the sliding block 4 in a sliding manner, the inner shell 6 is located in the outer shell 5 and is connected with the outer shell 5 in a sliding manner, the lower end of the inner shell 6 is provided with a suction nozzle 13, the lower extreme of shell body 5 is equipped with opening 14, and suction nozzle 13 passes opening 14, shell body 6 upper end can be dismantled with shell body 5 upper end and be connected, and piston 7 is arranged in interior casing 6 and with interior casing 6 seal fit, and piston 7 upper end is equipped with piston rod 15, be equipped with second rectangular hole 18 on top board 2, piston rod 15 upper end and second rectangular hole 18 are connected, during the use, follow first rectangular hole 3 earlier and adjust the position of extraction mechanism to the realization is extracted the semi-solid sample of different positions, later reciprocates shell body 5, and the degree of depth that different extraction mechanism lower extremes deepened semi-solid sample is different, realizes the sample of the different degree of depth of half fixed sample, later upwards lifts up top board 2, upwards stimulates piston 7, once can accomplish the synchronous sample of a plurality of extraction mechanisms, labour saving and time saving.
Second rectangular hole 18 and first rectangular hole 3 one-to-one are equipped with the clearance between interior casing 6 and the shell body 5, when preventing to take out the shell body, produce negative pressure suction between interior casing 6 and the shell body 5, inhale the shell body with the emulsion in, guaranteed the clean and tidy of shell body.
As shown in fig. 1, the support plate 1 is provided with three guide pillars 21, the upper platen 2 is provided with a guide sleeve 22, the guide pillars 21 are matched with the guide sleeve 22, the upper end of the guide pillar 21 is provided with a limit nut 23, the guide pillar 21 is provided with an external thread, the limit nut 23 is in threaded connection with the guide pillar 21, the upper platen 2 can move up and down along the guide pillar 21, the position of the limit nut 23 can be adjusted up and down, so that the upward pulling distance of the upper platen 2 is adjusted, a sample with a fixed volume is obtained, and the experiment precision is high.
As shown in fig. 3, the lower extreme of slider 4 is equipped with threaded sleeve 8, and threaded sleeve 8 passes first rectangular hole 3, and the slider 4 intermediate position is equipped with locating plate 9, and locating plate 9 and the contact of support flat board 1 up end are equipped with first nut 10 on the threaded sleeve 8, through the fixed slider 4 of first nut 10, can follow first rectangular hole 3 axis removal slider 4 with first nut 10 unscrewing, can realize the regulation of extraction mechanism position, take a sample to the semi-solid sample of different positions, the practicality is good.
As shown in fig. 3, be equipped with the through-hole in the slider 4, shell body 5 passes the through-hole, and slider 4 upper end is equipped with sleeve 11, is equipped with locking screw 12 on the sleeve 11, and shell body 5 can reciprocate in the through-hole, and through locking screw 12 locking, can take a sample to the different degree of depth of semi-solid sample, heightens the sampling precision.
As shown in fig. 3, a sealing ring is arranged between the suction nozzle 13 and the inner wall of the opening 14, so that the emulsion is prevented from entering the outer shell through a gap between the suction nozzle 13 and the opening 14, and the inside of the outer shell is not required to be cleaned for many times, which is time-consuming and labor-consuming and affects the reuse of the outer shell.
As shown in fig. 2, outer casing 5 upper end is equipped with spacing groove 16, interior casing 6 upper end is equipped with stopper 17, stopper 17 cooperates and passes through pin joint with spacing groove 16, during the installation, go deep into outer casing 5 with interior casing 6, spacing groove 16 cooperates with stopper 17, make and to rotate relatively between interior casing 6 and the outer casing 5, and can confirm completely through spacing groove 16 and stopper 17 whether the cooperation has impurity in the outer casing, and confirm whether interior casing is installed in place, later realize the axial positioning of interior casing and outer casing through the pin, prevent upwards extracting the piston, when drawing the sample, interior casing rises along with the piston. After the sample is accomplished, can take out interior casing 6 from shell body 5, conveniently take out the sample, simultaneously, the position between shell body and the support plate 1 can remain unchanged, can take a sample next time after the washing, need not adjust, and the practicality is good.
As shown in fig. 1, the upper end of the piston rod 15 is provided with a second nut 19, the upper end of the piston rod 15 passes through the second elongated hole 18, the second nut 19 is matched with the upper end surface of the upper pressure plate 2, and when the upper pressure plate 2 is lifted upwards, the upper end surface of the upper pressure plate 2 is contacted with the second nut 19, so that the piston rod 15 is pulled upwards, the piston generates negative pressure in the inner shell 6, and the emulsion is sucked into the inner shell.
As shown in FIG. 1, a handle 20 is disposed at the middle position of the upper press plate 2, so as to facilitate the upward pulling of the upper press plate 2.
As shown in figure 1, the lower end of the support plate 1 is provided with a support leg 24, and the support leg 24 is supported on the ground, so that the device is more stable when a sample is extracted.
In the description of the present invention, it should be noted that the terms "left", "right", "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; they may be mechanically or electrically connected, directly or indirectly through intervening media, or may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Claims (6)

1. The sampling device for detecting the microorganisms in the semisolid sample is characterized by comprising a supporting flat plate (1), an upper pressing plate (2) and an extracting mechanism, wherein a first strip hole (3) is formed in the supporting flat plate (1), a plurality of first strip holes (3) are uniformly formed in the circumferential direction of the supporting flat plate (1), the first strip holes (3) correspond to the extracting mechanism one by one, a sliding block (4) is arranged between the extracting mechanism and the first strip holes (3), the sliding block (4) is in sliding connection with the first strip holes (3), the extracting mechanism comprises an outer shell (5), an inner shell (6) and a piston (7), the outer shell (5) penetrates through the sliding block (4) and is in sliding connection with the sliding block (4), a threaded sleeve (8) is arranged at the lower end of the sliding block (4), the threaded sleeve (8) penetrates through the first strip holes (3), a positioning plate (9) is arranged in the middle position of the sliding block (4), the positioning plate (9) is in contact with the upper end face of the support flat plate (1), a first nut (10) is arranged on the threaded sleeve (8), a through hole is formed in the slider (4), the outer shell (5) penetrates through the through hole, a sleeve (11) is arranged at the upper end of the slider (4), a locking screw (12) is arranged on the sleeve (11), the inner shell (6) is positioned in the outer shell (5) and is in sliding connection with the outer shell (5), a suction nozzle (13) is arranged at the lower end of the inner shell (6), an opening (14) is formed at the lower end of the outer shell (5), the suction nozzle (13) penetrates through the opening (14), the upper end of the inner shell (6) is detachably connected with the upper end of the outer shell (5), the piston (7) is positioned in the inner shell (6) and is in sealing fit with the inner shell (6), a piston rod (15) is arranged at the upper end of the piston (7), a second long strip hole (18) is formed in the upper pressing plate (2), the upper end of the piston rod (15) is connected with the second strip hole (18);
the support plate (1) is provided with a guide pillar (21), the upper pressing plate (2) is provided with a guide sleeve (22), the guide pillar (21) is matched with the guide sleeve (22), the upper end of the guide pillar (21) is provided with a limit nut (23), and the limit nut (23) is in threaded connection with the guide pillar (21).
2. The sampling device for the microbiological test of semi-solid samples as claimed in claim 1, characterized in that a sealing ring is provided between said suction nozzle (13) and the inner wall of said opening (14).
3. The sampling device for detecting the microorganisms in the semisolid sample according to claim 1, wherein a limit groove (16) is arranged at the upper end of the outer shell (5), a limit block (17) is arranged at the upper end of the inner shell (6), and the limit block (17) is matched with the limit groove (16) and is connected with the limit groove through a pin.
4. The sampling device for detecting the microorganisms in the semisolid sample according to claim 1, wherein a second nut (19) is arranged at the upper end of the piston rod (15), the upper end of the piston rod (15) penetrates through the second elongated hole (18), and the second nut (19) is matched with the upper end surface of the upper pressure plate (2).
5. The sampling device for the microbiological test of semi-solid samples as claimed in claim 1, characterized in that said upper plate (2) is provided with a handle (20) in the middle.
6. A sampling device for microbiological testing of semi-solid samples according to claim 1 characterized in that said support plate (1) is provided with legs (24) at its lower end.
CN202010805770.9A 2020-08-12 2020-08-12 Sampling device for microbial detection of semisolid sample Active CN111893031B (en)

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CN111893031B true CN111893031B (en) 2022-03-22

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