CN111868032A - Kynurenine pathway inhibitors - Google Patents
Kynurenine pathway inhibitors Download PDFInfo
- Publication number
- CN111868032A CN111868032A CN201980012783.6A CN201980012783A CN111868032A CN 111868032 A CN111868032 A CN 111868032A CN 201980012783 A CN201980012783 A CN 201980012783A CN 111868032 A CN111868032 A CN 111868032A
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- YGPSJZOEDVAXAB-UHFFFAOYSA-N kynurenine Chemical compound OC(=O)C(N)CC(=O)C1=CC=CC=C1N YGPSJZOEDVAXAB-UHFFFAOYSA-N 0.000 title abstract description 30
- 230000037361 pathway Effects 0.000 title abstract description 12
- 239000003112 inhibitor Substances 0.000 title abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 216
- 150000003839 salts Chemical class 0.000 claims abstract description 39
- -1 2H-indazolyl Chemical group 0.000 claims description 91
- 125000005842 heteroatom Chemical group 0.000 claims description 44
- 229910052757 nitrogen Inorganic materials 0.000 claims description 35
- 206010028980 Neoplasm Diseases 0.000 claims description 21
- 239000003814 drug Substances 0.000 claims description 15
- 125000004404 heteroalkyl group Chemical group 0.000 claims description 14
- 125000002023 trifluoromethyl group Chemical group FC(F)(F)* 0.000 claims description 14
- 125000000753 cycloalkyl group Chemical group 0.000 claims description 11
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 11
- 125000000592 heterocycloalkyl group Chemical group 0.000 claims description 11
- 229910052794 bromium Inorganic materials 0.000 claims description 10
- 229910052801 chlorine Inorganic materials 0.000 claims description 10
- 229910052740 iodine Inorganic materials 0.000 claims description 10
- 229910052731 fluorine Inorganic materials 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- JNCMHMUGTWEVOZ-UHFFFAOYSA-N F[CH]F Chemical compound F[CH]F JNCMHMUGTWEVOZ-UHFFFAOYSA-N 0.000 claims description 8
- VUWZPRWSIVNGKG-UHFFFAOYSA-N fluoromethane Chemical compound F[CH2] VUWZPRWSIVNGKG-UHFFFAOYSA-N 0.000 claims description 8
- 125000004076 pyridyl group Chemical group 0.000 claims description 8
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 7
- 201000011510 cancer Diseases 0.000 claims description 6
- 229910052736 halogen Inorganic materials 0.000 claims description 6
- 150000002367 halogens Chemical class 0.000 claims description 6
- VOLGAXAGEUPBDM-UHFFFAOYSA-N $l^{1}-oxidanylethane Chemical compound CC[O] VOLGAXAGEUPBDM-UHFFFAOYSA-N 0.000 claims description 4
- 229910003204 NH2 Inorganic materials 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- GRVDJDISBSALJP-UHFFFAOYSA-N methyloxidanyl Chemical compound [O]C GRVDJDISBSALJP-UHFFFAOYSA-N 0.000 claims description 4
- 239000008194 pharmaceutical composition Substances 0.000 claims description 4
- 125000005955 1H-indazolyl group Chemical group 0.000 claims description 3
- 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 claims description 3
- 125000001041 indolyl group Chemical group 0.000 claims description 3
- 125000002183 isoquinolinyl group Chemical group C1(=NC=CC2=CC=CC=C12)* 0.000 claims description 3
- 125000002943 quinolinyl group Chemical group N1=C(C=CC2=CC=CC=C12)* 0.000 claims description 3
- 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims description 2
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 114
- 239000000203 mixture Substances 0.000 description 95
- 239000000243 solution Substances 0.000 description 95
- 239000012043 crude product Substances 0.000 description 81
- 230000015572 biosynthetic process Effects 0.000 description 72
- 238000003786 synthesis reaction Methods 0.000 description 70
- 238000006243 chemical reaction Methods 0.000 description 67
- IAZDPXIOMUYVGZ-WFGJKAKNSA-N Dimethyl sulfoxide Chemical compound [2H]C([2H])([2H])S(=O)C([2H])([2H])[2H] IAZDPXIOMUYVGZ-WFGJKAKNSA-N 0.000 description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 54
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 53
- 239000012074 organic phase Substances 0.000 description 53
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 49
- 238000001914 filtration Methods 0.000 description 48
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 45
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 44
- 235000019439 ethyl acetate Nutrition 0.000 description 43
- 238000001035 drying Methods 0.000 description 41
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical class O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 41
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 39
- 229910052938 sodium sulfate Inorganic materials 0.000 description 35
- 238000005160 1H NMR spectroscopy Methods 0.000 description 34
- 239000007832 Na2SO4 Substances 0.000 description 34
- JGFZNNIVVJXRND-UHFFFAOYSA-N N,N-Diisopropylethylamine (DIPEA) Chemical compound CCN(C(C)C)C(C)C JGFZNNIVVJXRND-UHFFFAOYSA-N 0.000 description 33
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 32
- 238000004237 preparative chromatography Methods 0.000 description 30
- 125000000217 alkyl group Chemical group 0.000 description 24
- 239000007821 HATU Substances 0.000 description 23
- KDLHZDBZIXYQEI-UHFFFAOYSA-N palladium Substances [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 23
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 20
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 19
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 19
- 125000001424 substituent group Chemical group 0.000 description 19
- 125000003118 aryl group Chemical group 0.000 description 18
- 125000002619 bicyclic group Chemical group 0.000 description 16
- WRIKHQLVHPKCJU-UHFFFAOYSA-N sodium bis(trimethylsilyl)amide Chemical compound C[Si](C)(C)N([Na])[Si](C)(C)C WRIKHQLVHPKCJU-UHFFFAOYSA-N 0.000 description 15
- 239000000706 filtrate Substances 0.000 description 14
- 239000011541 reaction mixture Substances 0.000 description 14
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 13
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 13
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 12
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 12
- 239000000376 reactant Substances 0.000 description 12
- 230000002829 reductive effect Effects 0.000 description 12
- 238000005406 washing Methods 0.000 description 12
- 125000003342 alkenyl group Chemical group 0.000 description 11
- 239000008346 aqueous phase Substances 0.000 description 11
- 125000004429 atom Chemical group 0.000 description 11
- 239000000460 chlorine Substances 0.000 description 11
- 229940079593 drug Drugs 0.000 description 11
- 239000012299 nitrogen atmosphere Substances 0.000 description 11
- 229910052717 sulfur Inorganic materials 0.000 description 11
- 239000002253 acid Substances 0.000 description 10
- 125000000304 alkynyl group Chemical group 0.000 description 10
- 229910052760 oxygen Inorganic materials 0.000 description 10
- 125000000392 cycloalkenyl group Chemical group 0.000 description 9
- 125000001072 heteroaryl group Chemical group 0.000 description 9
- 125000004366 heterocycloalkenyl group Chemical group 0.000 description 9
- KRTIYQIPSAGSBP-KLAILNCOSA-N linrodostat Chemical compound C1(CCC(CC1)C1=C2C=C(F)C=CC2=NC=C1)[C@@H](C)C(=O)NC1=CC=C(Cl)C=C1 KRTIYQIPSAGSBP-KLAILNCOSA-N 0.000 description 9
- 125000002950 monocyclic group Chemical group 0.000 description 9
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 9
- KZPYGQFFRCFCPP-UHFFFAOYSA-N 1,1'-bis(diphenylphosphino)ferrocene Chemical compound [Fe+2].C1=CC=C[C-]1P(C=1C=CC=CC=1)C1=CC=CC=C1.C1=CC=C[C-]1P(C=1C=CC=CC=1)C1=CC=CC=C1 KZPYGQFFRCFCPP-UHFFFAOYSA-N 0.000 description 8
- PFKFTWBEEFSNDU-UHFFFAOYSA-N carbonyldiimidazole Chemical compound C1=CN=CN1C(=O)N1C=CN=C1 PFKFTWBEEFSNDU-UHFFFAOYSA-N 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000000605 extraction Methods 0.000 description 8
- 239000012065 filter cake Substances 0.000 description 8
- 229910052739 hydrogen Inorganic materials 0.000 description 8
- 239000001257 hydrogen Substances 0.000 description 8
- 125000006239 protecting group Chemical group 0.000 description 8
- 238000006467 substitution reaction Methods 0.000 description 8
- 125000004434 sulfur atom Chemical group 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 description 7
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical group C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 7
- 125000004122 cyclic group Chemical group 0.000 description 7
- 125000000524 functional group Chemical group 0.000 description 7
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 6
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 6
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 239000012298 atmosphere Substances 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 125000004432 carbon atom Chemical group C* 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 239000011734 sodium Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 6
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical class [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 5
- 241000699670 Mus sp. Species 0.000 description 5
- 125000003545 alkoxy group Chemical group 0.000 description 5
- 125000003710 aryl alkyl group Chemical group 0.000 description 5
- 229910052796 boron Inorganic materials 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 125000005647 linker group Chemical group 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000003981 vehicle Substances 0.000 description 5
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical class [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 125000005110 aryl thio group Chemical group 0.000 description 4
- 125000004104 aryloxy group Chemical group 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 238000004440 column chromatography Methods 0.000 description 4
- 125000004475 heteroaralkyl group Chemical group 0.000 description 4
- 125000005553 heteroaryloxy group Chemical group 0.000 description 4
- 125000005368 heteroarylthio group Chemical group 0.000 description 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- HCZHHEIFKROPDY-UHFFFAOYSA-N kynurenic acid Chemical compound C1=CC=C2NC(C(=O)O)=CC(=O)C2=C1 HCZHHEIFKROPDY-UHFFFAOYSA-N 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 4
- JLFNLZLINWHATN-UHFFFAOYSA-N pentaethylene glycol Chemical compound OCCOCCOCCOCCOCCO JLFNLZLINWHATN-UHFFFAOYSA-N 0.000 description 4
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- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 description 4
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- 238000010189 synthetic method Methods 0.000 description 4
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- 238000002560 therapeutic procedure Methods 0.000 description 4
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 4
- CXNIUSPIQKWYAI-UHFFFAOYSA-N xantphos Chemical compound C=12OC3=C(P(C=4C=CC=CC=4)C=4C=CC=CC=4)C=CC=C3C(C)(C)C2=CC=CC=1P(C=1C=CC=CC=1)C1=CC=CC=C1 CXNIUSPIQKWYAI-UHFFFAOYSA-N 0.000 description 4
- YPBKTZBXSBLTDK-PKNBQFBNSA-N (3e)-3-[(3-bromo-4-fluoroanilino)-nitrosomethylidene]-4-[2-(sulfamoylamino)ethylamino]-1,2,5-oxadiazole Chemical compound NS(=O)(=O)NCCNC1=NON\C1=C(N=O)/NC1=CC=C(F)C(Br)=C1 YPBKTZBXSBLTDK-PKNBQFBNSA-N 0.000 description 3
- UIADWSXPNFQQCZ-UHFFFAOYSA-N 4-[4-(3,4-dichlorophenyl)-5-phenyl-1,3-oxazol-2-yl]butanoic acid Chemical compound ClC=1C=C(C=CC=1Cl)C=1N=C(OC=1C1=CC=CC=C1)CCCC(=O)O UIADWSXPNFQQCZ-UHFFFAOYSA-N 0.000 description 3
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- 125000002971 oxazolyl group Chemical group 0.000 description 1
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- 125000003566 oxetanyl group Chemical group 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 125000006340 pentafluoro ethyl group Chemical group FC(F)(F)C(F)(F)* 0.000 description 1
- 125000002255 pentenyl group Chemical group C(=CCCC)* 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 125000005981 pentynyl group Chemical group 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
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- 125000004483 piperidin-3-yl group Chemical group N1CC(CCC1)* 0.000 description 1
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- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 125000006684 polyhaloalkyl group Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- 239000002244 precipitate Substances 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 125000004368 propenyl group Chemical group C(=CC)* 0.000 description 1
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- OSFBJERFMQCEQY-UHFFFAOYSA-N propylidene Chemical compound [CH]CC OSFBJERFMQCEQY-UHFFFAOYSA-N 0.000 description 1
- 125000002568 propynyl group Chemical group [*]C#CC([H])([H])[H] 0.000 description 1
- 125000003072 pyrazolidinyl group Chemical group 0.000 description 1
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- 230000006340 racemization Effects 0.000 description 1
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- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
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- 239000012279 sodium borohydride Substances 0.000 description 1
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- ISIJQEHRDSCQIU-UHFFFAOYSA-N tert-butyl 2,7-diazaspiro[4.5]decane-7-carboxylate Chemical compound C1N(C(=O)OC(C)(C)C)CCCC11CNCC1 ISIJQEHRDSCQIU-UHFFFAOYSA-N 0.000 description 1
- 125000004192 tetrahydrofuran-2-yl group Chemical group [H]C1([H])OC([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
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- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
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- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 125000001425 triazolyl group Chemical group 0.000 description 1
- 125000003866 trichloromethyl group Chemical group ClC(Cl)(Cl)* 0.000 description 1
- ITMCEJHCFYSIIV-UHFFFAOYSA-M triflate Chemical compound [O-]S(=O)(=O)C(F)(F)F ITMCEJHCFYSIIV-UHFFFAOYSA-M 0.000 description 1
- 125000004044 trifluoroacetyl group Chemical group FC(C(=O)*)(F)F 0.000 description 1
- LEIMLDGFXIOXMT-UHFFFAOYSA-N trimethylsilyl cyanide Chemical compound C[Si](C)(C)C#N LEIMLDGFXIOXMT-UHFFFAOYSA-N 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
- 125000002221 trityl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C([*])(C1=C(C(=C(C(=C1[H])[H])[H])[H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- 230000005909 tumor killing Effects 0.000 description 1
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D217/00—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems
- C07D217/02—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with only hydrogen atoms or radicals containing only carbon and hydrogen atoms, directly attached to carbon atoms of the nitrogen-containing ring; Alkylene-bis-isoquinolines
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Abstract
The invention discloses a novel compound serving as a kynurenine pathway inhibitor, and particularly discloses a compound shown as a formula (I) and a pharmaceutically acceptable salt thereof.
Description
The following priority is claimed in the present application:
CN201810143151.0, application date 2018, 2 month 11.
The invention relates to a novel compound serving as a kynurenine pathway inhibitor, and particularly discloses a compound shown as a formula (I) and a pharmaceutically acceptable salt thereof. The invention also relates to application of the compound shown in the formula (I) and pharmaceutically acceptable salts thereof in preparing medicaments for treating tumors.
Malignant tumors are now one of the major diseases that jeopardize human life safety. In recent centuries, in order to combat malignant tumors, many diagnostic and therapeutic approaches including most common chemotherapy, surgery, radiotherapy and targeted therapy have been developed. These therapies delay the development of tumors to some extent, prolonging patient life. However, due to the unlimited growth, infiltration and metastasis characteristics of malignant tumors, the above therapies still fail to achieve the desired inhibitory effect. Meanwhile, the toxic and side effects of the above therapies are also key factors limiting the application thereof.
In recent years, immunotherapy has been developed as an emerging therapy for the treatment of malignant tumors, characterized by combating the growth of tumors by mobilizing the host's natural defense mechanisms. The main approach is to enhance the host collective immune response to tumor cells by activating the original immune system of the host, and to achieve accurate and effective tumor killing by using the immune system capability, thereby inhibiting the development of the tumor.
The production of kynurenic acid inside tumors is mainly regulated by Indoleamine 2,3-dioxygenase (IDO) and Tryptophan-2, 3-dioxygenase (TDO), which function to catalyze the rate-determining step of Tryptophan metabolism to kynurenic acid. The above enzymes can rapidly consume tryptophan in a tumor microenvironment, so that T cells infiltrated into the tumor are stagnated in the middle stage of G1 due to the lack of tryptophan, thereby inhibiting the proliferation of the T cells and preventing the signal conduction of the T cells and losing the functions. At the same time, the concentration of kynurenine is increased, which leads to the formation of an immunosuppressive microenvironment by the tumor. Therefore, inhibition of kynurenine production in tumors helps activate the collective immune system, counteracting tumor growth.
The kynurenine pathway inhibitor has good application prospect in the pharmaceutical industry as a medicament, but no kynurenine pathway inhibitor is on the market at present. The clinical kynurenine pathway inhibitors are (D) -1-methyltryptophan developed by NewLink Genetics, NLG-0919, INCB-24360 from Incyte, and BMS-985205 from Bethes. However, the existing clinical kynurenine pathway inhibitor has the problems of CYP inhibition (NLG 0919), large administration dose, short half-life, more administration times (INCB-24360), poor solubility (BMS-986205) and the like. Therefore, kynurenine pathway inhibitors that do not have the above disadvantages remain unmet medical needs.
The specific structures of NLG-0919, INCB-24360 and BMS-986205 are as follows:
disclosure of Invention
The invention provides a compound shown in a formula (I) or a pharmaceutically acceptable salt thereof,
wherein the content of the first and second substances,
ring A is selected from the group consisting of phenyl, pyridyl, cyclopentyl 1H-indazolyl, 2H-indazolyl, 1H-indolyl and cyclohexyl;
ring B is selected from phenyl, pyridyl, quinolinyl, isoquinolinyl, 1H-indazolyl, and 2H-indazolyl;
l is selected from a single bond, -O-, -CH2-、-CH2CH2-and-CH2CH2CH2-;
X is selected from CH and N;
l' is selected from a single bond and-O-;
R1each independently selected from halogen and NH2、CN、C1-3Alkyl, CF3、CHF2、CH2F、C1-3A heteroalkyl group;
R2each independently selected from halogen, CN, C1-3Alkyl, CF3、CHF2、CH2F、C1-3Heteroalkyl, 3-6 membered cycloalkyl, 3-6 membered heterocycloalkyl, 3-6 membered cycloalkyl-O-, 3-6 membered heterocycloalkyl-O-, 3-6 membered cycloalkyl-NH-, 3-6 membered heterocycloalkyl-NH-;
m and n are each independently selected from 0, 1,2 and 3;
"hetero" means a heteroatom or a heteroatom group, said C1-3"hetero" of heteroalkyl, 3-6 membered heterocycloalkyl-O-, and 3-6 membered heterocycloalkyl-NH-is selected from the group consisting of N, -NH-, -O-, -S-, -C (═ O) -, -C (═ S) -, -C (═ O) O-, -S (═ O) -, and-S (═ O)2-;
In any of the above cases, the number of heteroatoms or heteroatom groups is independently selected from 1,2 and 3, respectively.
In some embodiments of the invention, R is as defined above1Each independently selected from F, Cl, Br, I, NH2、CN、CH3、CF3、CH3CH2、CH3O and CH3CH2O。
In some embodiments of the invention, R is as defined above2Each independently selected from F, Cl, Br, I, CN, CH3、CF3、CHF2、CH2F、CH3CH2、CH3O-、CH3CH2O-、
In some embodiments of the invention, R is as defined above1Each independently selected from F, Cl, Br, I, NH2、CN、CH3、CF3、CH3CH2、CH3O and CH3CH2O, other variables are as defined above.
In some embodiments of the invention, R is as defined above2Each independently selected from F, Cl, Br, I, CN, CH3、CF3、CHF2、CH2F、CH3CH2、CH3O-、CH3CH2O-、The other variables are as defined above.
In some embodiments of the invention, the structural unitIs selected fromThe other variables are as defined above.
In some embodiments of the invention, the structural unitIs selected from The other variables are as defined above.
In some embodiments of the invention, the structural unitIs selected from The other variables are as defined above.
In some embodiments of the invention, the structural unitIs selected from The other variables are as defined above.
In some embodiments of the invention, the structural unitIs selected from Other variables are as aboveThe above definitions.
In some embodiments of the invention, the compound, or a pharmaceutically acceptable salt thereof, is selected from
Wherein, ring A, X, m, n, L', R1And R2As defined above.
In some embodiments of the invention, the compound, or a pharmaceutically acceptable salt thereof, is selected from
Wherein, L, R1And R2As defined above.
In some embodiments of the invention, R is as defined above1Each independently selected from F, Cl, Br, I, NH2、CN、CH3、CF3、CH3CH2、CH3O and CH3CH2O, other variables are as defined herein.
In some embodiments of the invention, R is as defined above2Each independently selected from F, Cl, Br, I, CN, CH3、CF3、CHF2、CH2F、CH3CH2、CH3O-、CH3CH2O-、The other variables are as defined above.
In some embodiments of the invention, the structural unitIs selected fromOther variables are as defined herein.
In some embodiments of the invention, the structural unitIs selected from Other variables are as defined herein.
In some embodiments of the invention, the structural unitIs selected from Other variables are as defined herein.
In some embodiments of the invention, the structural unitIs selected from Other variables are as defined herein.
In some embodiments of the invention, the structural unitIs selected from Other variables are as defined herein.
In some embodiments of the invention, the compound, or a pharmaceutically acceptable salt thereof, is selected from
Wherein, ring A, m, n, X, L', R1And R2As defined herein.
10. A compound according to claim 9, or a pharmaceutically acceptable salt thereof, selected from
Wherein L is,R1And R2As defined herein.
The invention also provides a compound or a pharmaceutically acceptable salt thereof selected from
The invention provides a pharmaceutical composition which comprises a therapeutically effective amount of the compound or the pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.
The invention also provides application of the compound or the pharmaceutically acceptable salt thereof or the pharmaceutical composition in preparing a medicament for treating cancer.
Still other embodiments of the present invention are derived from any combination of the above variables.
Technical effects
The invention provides a kynurenine pathway inhibitor with a novel structure, which has excellent in vitro cell activity; the in vivo metabolic stability of the series of compounds can be enhanced by the cyclic structure in the ortho position of the amide in the structure. At the same dose, the structure of the invention has a higher AUC than BMS-986205, and the half-life is longer. Meanwhile, compared with BMS-986205, the series of compounds have better solubility, higher oral bioavailability, better tissue distribution in mice and better comprehensive pharmacokinetic property. The novel structures provided by the present invention are likely to be selective IDO inhibitors.
Definitions and explanations
As used herein, the following terms and phrases are intended to have the following meanings, unless otherwise indicated. A particular term or phrase, unless specifically defined, should not be considered as indefinite or unclear, but rather construed according to ordinary meaning. When a trade name appears herein, it is intended to refer to its corresponding commodity or its active ingredient. The term "pharmaceutically acceptable" as used herein is intended to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
The term "pharmaceutically acceptable salts" refers to salts of the compounds of the present invention, prepared from the compounds of the present invention found to have particular substituents, with relatively nontoxic acids or bases. When compounds of the present invention contain relatively acidic functional groups, base addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of a base in neat solution or in a suitable inert solvent. Pharmaceutically acceptable base addition salts include sodium, potassium, calcium, ammonium, organic amines or magnesium salts or similar salts. When compounds of the present invention contain relatively basic functional groups, acid addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of acid in neat solution or in a suitable inert solvent. Examples of pharmaceutically acceptable acid addition salts include inorganic acid salts including, for example, hydrochloric acid, hydrobromic acid, nitric acid, carbonic acid, bicarbonate, phosphoric acid, monohydrogen phosphate, dihydrogen phosphate, sulfuric acid, hydrogen sulfate, hydroiodic acid, phosphorous acid, and the like; and salts of organic acids including acids such as acetic, propionic, isobutyric, maleic, malonic, benzoic, succinic, suberic, fumaric, lactic, mandelic, phthalic, benzenesulfonic, p-toluenesulfonic, citric, tartaric, methanesulfonic, and the like; also included are salts of amino acids such as arginine and the like, and salts of organic acids such as glucuronic acid and the like. Certain specific compounds of the invention contain both basic and acidic functionalities and can thus be converted to any base or acid addition salt.
The pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound, which contains an acid or base, by conventional chemical methods. In general, such salts are prepared by the following method: prepared by reacting these compounds in free acid or base form with a stoichiometric amount of the appropriate base or acid, in water or an organic solvent or a mixture of the two.
In addition to salt forms, the compounds provided herein also exist in prodrug forms. Prodrugs of the compounds described herein readily undergo chemical changes under physiological conditions to convert to the compounds of the present invention. In addition, prodrugs can be converted to the compounds of the present invention in an in vivo environment by chemical or biochemical means.
Certain compounds of the present invention may exist in unsolvated forms as well as solvated forms, including hydrated forms. In general, the solvated forms are equivalent to unsolvated forms and are intended to be encompassed within the scope of the present invention.
The compounds of the present invention may exist in specific geometric or stereoisomeric forms. The present invention contemplates all such compounds, including cis and trans isomers, (-) -and (+) -enantiomers, (R) -and (S) -enantiomers, diastereomers, (D) -isomers, (L) -isomers, as well as racemic and other mixtures thereof, such as enantiomerically or diastereomerically enriched mixtures, all of which are within the scope of the present invention. Additional asymmetric carbon atoms may be present in substituents such as alkyl groups. All such isomers, as well as mixtures thereof, are included within the scope of the present invention.
Unless otherwise indicated, the terms "enantiomer" or "optical isomer" refer to stereoisomers that are mirror images of each other.
Unless otherwise indicated, the term "cis-trans isomer" or "geometric isomer" results from the inability of a double bond or a single bond to rotate freely within a ring-forming carbon atom.
Unless otherwise indicated, the term "diastereomer" refers to a stereoisomer in which the molecules have two or more chiral centers and a non-mirror image relationship between the molecules.
Unless otherwise indicated, "(D)" or "(+)" means dextrorotation, "(L)" or "(-) -means levorotation," (DL) "or" (±) "means racemization.
Using solid wedge keys, unless otherwise indicatedAnd wedge dotted bondShowing the absolute configuration of a solid centre, by means of straight solid keysAnd straight dotted line bondShowing the relative configuration of the centres of solids, by wavy linesRepresenting solid-line keys of wedge shapeOr wedge dotted bondOr by wavy linesIndicating straight solid-line keysAnd straight dotted line bond
The compounds of the invention may be present specifically. Unless otherwise indicated, the term "tautomer" or "tautomeric form" means that at room temperature, the isomers of different functional groups are in dynamic equilibrium and can be rapidly interconverted. If tautomers are possible (e.g., in solution), then the chemical equilibrium of the tautomers can be reached. For example, proton tautomers (prototropic tautomers), also known as proton transfer tautomers (prototropic tautomers), include interconversions by proton transfer, such as keto-enol isomerization and imine-enamine isomerization. Valence isomers (valencetatomer) include interconversion by recombination of some of the bonding electrons. A specific example of where keto-enol tautomerism is the interconversion between two tautomers of pentane-2, 4-dione and 4-hydroxypent-3-en-2-one.
Unless otherwise indicated, the terms "enriched in one isomer", "isomer enriched", "enantiomer enriched" or "enantiomeric enrichment" refer to a content of one isomer or enantiomer of less than 100%, and the content of the isomer or enantiomer is greater than or equal to 60%, or greater than or equal to 70%, or greater than or equal to 80%, or greater than or equal to 90%, or greater than or equal to 95%, or greater than or equal to 96%, or greater than or equal to 97%, or greater than or equal to 98%, or greater than or equal to 99%, or greater than or equal to 99.5%, or greater than or equal to 99.6%, or greater than or equal to 99.7%, or greater than or equal to 99.8%, or greater than or equal to 99.9%.
Unless otherwise indicated, the term "isomeric excess" or "enantiomeric excess" refers to the difference between the relative percentages of two isomers or enantiomers. For example, if the content of one isomer or enantiomer is 90%, and the content of the other isomer or enantiomer is 10%, the isomer or enantiomer excess (ee value) is 80%.
Optically active (R) -and (S) -isomers as well as D and L isomers can be prepared by chiral synthesis or chiral reagents or other conventional techniques.If one of the enantiomers of a compound of the invention is desired, it can be prepared by asymmetric synthesis or derivatization with a chiral auxiliary, wherein the resulting diastereomeric mixture is separated and the auxiliary group is cleaved to provide the pure desired enantiomer. Alternatively, when the molecule contains a basic functional group (e.g., amino) or an acidic functional group (e.g., carboxyl), diastereomeric salts are formed with an appropriate optically active acid or base, followed by diastereomeric resolution by conventional methods known in the art, and the pure enantiomers are recovered. Furthermore, separation of enantiomers and diastereomers is typically accomplished by using chromatography employing a chiral stationary phase, optionally in combination with chemical derivatization (e.g., carbamate formation from amines). The compounds of the present invention may contain unnatural proportions of atomic isotopes at one or more of the atoms that constitute such compounds. For example, the compounds may be labelled with radioactive isotopes, such as tritium (A), (B), (C3H) Iodine-125 (125I) Or C-14(14C) In that respect For example, deuterium can be used to replace hydrogen to form a deuterated drug, the bond formed by deuterium and carbon is stronger than the bond formed by common hydrogen and carbon, and compared with an undeuterated drug, the deuterated drug has the advantages of reducing toxic and side effects, increasing the stability of the drug, enhancing the curative effect, prolonging the biological half-life period of the drug and the like. All isotopic variations of the compounds of the present invention, whether radioactive or not, are intended to be encompassed within the scope of the present invention. "optional" or "optionally" means that the subsequently described event or circumstance may, but need not, occur, and that the description includes instances where said event or circumstance occurs and instances where it does not.
The term "substituted" means that any one or more hydrogen atoms on a particular atom is replaced with a substituent, and may include variations of deuterium and hydrogen, so long as the valency of the particular atom is normal and the substituted compound is stable. When the substituent is oxygen (i.e., ═ O), it means that two hydrogen atoms are substituted. Oxygen substitution does not occur on aromatic groups. The term "optionally substituted" means that it may or may not be substituted, and unless otherwise specified, the kind and number of substituents may be arbitrary on the basis of chemical realizability.
When any variable (e.g., R) occurs more than one time in the composition or structure of a compound, its definition in each case is independent. Thus, for example, if a group is substituted with 0-2R, the group may optionally be substituted with up to two R, and there are separate options for R in each case. Furthermore, combinations of substituents and/or variants thereof are permissible only if such combinations result in stable compounds.
When the number of one linking group is 0, e.g. - (CRR)0-, represents that the linking group is a single bond.
When one of the variables is selected from a single bond, it means that the two groups to which it is attached are directly connected, for example, where L represents a single bond in A-L-Z means that the structure is actually A-Z.
When a substituent is absent, it indicates that the substituent is absent, e.g., when X is absent in A-X, it indicates that the structure is actually A. When no atom through which a substituent is attached to a substituted group is indicated in the listed substituents, such substituents may be bonded through any atom thereof, for example, a pyridyl group as a substituent may be attached to a substituted group through any one of carbon atoms on the pyridine ring.
When the listed linking groups do not indicate their direction of attachment, the direction of attachment is arbitrary, for example,wherein the linking group L is-M-W-, in which case-M-W-can be formed by connecting the ring A and the ring B in the same direction as the reading sequence from left to rightThe ring A and the ring B may be connected in the reverse direction of the reading sequence from left to rightCombinations of the linking groups, substituents, and/or variants thereof are permissible only if such combinations result in stable compounds.
Unless otherwise specified, the term "hetero" denotes a heteroatom or a heteroatom group (i.e., a heteroatom-containing radical) including atoms other than carbon (C) and hydrogen (H) and radicals containing such heteroatoms, including, for example, oxygen (O), nitrogen (N), sulfur (S), silicon (Si), germanium (Ge), aluminum (Al), boron (B), -O-, -S-, -C (═ O) O-, -C (═ O) -, -C (═ S) -, -S (═ O)2-, and optionally substituted-C (═ O) n (h) -, -C (═ NH) -, -S (═ O)2N (h) -or-S (═ O) n (h) -.
Unless otherwise specified, "cyclic" means substituted or unsubstituted cycloalkyl, heterocycloalkyl, cycloalkenyl, heterocycloalkenyl, cycloalkynyl, heterocycloalkynyl, aryl, or heteroaryl. The ring includes monocyclic ring, and also includes bicyclic or polycyclic ring systems such as spiro ring, fused ring and bridged ring. The number of atoms in the ring is generally defined as the number of ring members, for example, "5 to 7 membered ring" means 5 to 7 atoms arranged around the ring. Unless otherwise specified, the ring optionally contains 1-3 heteroatoms. Thus, "5-to 7-membered ring" includes, for example, phenyl, pyridyl and piperidinyl; on the other hand, the term "5 to 7 membered heterocycloalkyl" includes pyridyl and piperidyl, but does not include phenyl. The term "ring" also includes ring systems containing at least one ring, each of which "ring" independently conforms to the above definition.
Unless otherwise specified, the term "alkyl" is used to denote a straight or branched chain saturated hydrocarbon group, in some embodiments the alkyl group is C1-12An alkyl group; in other embodiments, the alkyl is C1-6An alkyl group; in other embodiments, the alkyl is C1-3An alkyl group. It may be monosubstituted (e.g. -CH)2F) Or polysubstituted (e.g. -CF)3) And may be monovalent (e.g., methyl), divalent (e.g., methylene), or polyvalent (e.g., methine). Examples of alkyl groups include, but are not limited to, methyl (Me), ethyl (Et), propyl (including n-propyl and isopropyl), butyl (clathrated)Including n-butyl, isobutyl, s-butyl and t-butyl), pentyl (including n-pentyl, isopentyl and neopentyl), hexyl and the like.
Unless otherwise specified, "alkenyl" is used to indicate a straight or branched hydrocarbon group containing one or more carbon-carbon double bonds, which may be located anywhere in the group. In some embodiments, the alkenyl is C2-8An alkenyl group; in other embodiments, the alkenyl group is C2-6An alkenyl group; in other embodiments, the alkenyl group is C2-4An alkenyl group. It may be mono-or poly-substituted, and may be mono-, di-or polyvalent. Examples of alkenyl groups include, but are not limited to, ethenyl, propenyl, butenyl, pentenyl, hexenyl, butadienyl, piperylene, hexadienyl, and the like.
Unless otherwise specified, "alkynyl" is used to denote a straight or branched hydrocarbon group containing one or more carbon-carbon triple bonds, which may be located anywhere in the group. In some embodiments, the alkynyl is C2-8An alkynyl group; in other embodiments, the alkynyl is C2-6An alkynyl group; in other embodiments, the alkynyl is C2-4Alkynyl. It may be mono-or poly-substituted, and may be mono-, di-or polyvalent. Examples of alkynyl groups include, but are not limited to, ethynyl, propynyl, butynyl, pentynyl, and the like.
Unless otherwise specified, the term "heteroalkyl," by itself or in combination with another term, means a stable straight or branched chain alkyl radical consisting of a number of carbon atoms and at least one heteroatom or heteroatom group, or combinations thereof. In some embodiments, the heteroatom is selected from B, O, N and S, wherein the nitrogen and sulfur atoms are optionally oxidized and the nitrogen heteroatom is optionally quaternized. In other embodiments, the heteroatom group is selected from-C (═ O) O-, -C (═ O) -, -C (═ S) -, -S (═ O)2-、-C(=O)N(H)-、-N(H)-、-C(=NH)-、-S(=O)2N (h) -and-S (═ O) n (h) -. In some embodiments, the heteroalkyl is C1-6A heteroalkyl group; in other embodiments, the heteroalkyl is C1-3A heteroalkyl group. The heteroatom or heteroatom group may be located at any internal position of the heteroalkyl group, including the position of attachment of the alkyl group to the remainder of the molecule, but the terms "alkoxy", "alkylamino" and "alkylthio" (or thioalkoxy) are used conventionally to refer to those alkyl groups that are attached to the remainder of the molecule through an oxygen atom, an amino group, or a sulfur atom, respectively. Examples of heteroalkyl groups include, but are not limited to, -OCH3、-OCH2CH3、-OCH2CH2CH3、-OCH2(CH3)2、-CH2-CH2-O-CH3、-NHCH3、-N(CH3)2、-NHCH2CH3、-N(CH3)(CH2CH3)、-CH2-CH2-NH-CH3、-CH2-CH2-N(CH3)-CH3、-SCH3、-SCH2CH3、-SCH2CH2CH3、-SCH2(CH3)2、-CH2-S-CH2-CH3、-CH2-CH2、-S(=O)-CH3、-CH2-CH2-S(=O)2-CH3、-CH=CH-O-CH3、-CH2-CH=N-OCH3and-CH ═ CH-N (CH)3)-CH3. Up to two heteroatoms may be consecutive, e.g. -CH2-NH-OCH3。
Unless otherwise specified, the term "heteroalkenyl" by itself or in combination with another term means a stable, straight or branched chain alkenyl radical, or combination thereof, consisting of a number of carbon atoms and at least one heteroatom or heteroatom group. In some embodiments, the heteroatom is selected from B, O, N and S, wherein the nitrogen and sulfur atoms are optionally oxidized and the nitrogen heteroatom is optionally quaternized. In other embodiments, the heteroatom group is selected from-C (═ O) O-, -C (═ O) -, -C (═ S) -, -S (═ O)2-、-C(=O)N(H)-、-N(H)-、-C(=NH)-、-S(=O)2N (h) -and-S (═ O) n (h) -. In some embodiments, the heteroalkenyl is C2-6A heteroalkenyl group; in yet other embodiments of the present invention, the substrate is,the heteroalkyl group is C2-4A heteroalkenyl group. The heteroatom or heteroatom group may be located at any internal position of the heteroalkenyl group, including the position of attachment of the alkenyl group to the rest of the molecule, but the terms "alkenyloxy", "alkenylamino" and "alkenylthio" are to be taken in their customary expression and refer to those alkenyl groups which are attached to the rest of the molecule via an oxygen atom, an amino group or a sulfur atom, respectively. Examples of heteroalkenyl include, but are not limited to, -O-CH ═ CH2、-O-CH=CHCH3、-O-CH=C(CH3)2、-CH=CH-O-CH3、-O-CH=CHCH2CH3、-CH2-CH=CH-OCH3、-NH-CH=CH2、-N(CH=CH2)- CH3、-CH=CH-NH-CH3、-CH=CH-N(CH3)2、-S-CH=CH2、-S-CH=CHCH3、-S-CH=C(CH3)2、-CH2-S-CH=CH2、-S(=O)-CH=CH2and-CH-S (O)2-CH3. Up to two heteroatoms may be consecutive, e.g. -CH-NH-OCH3。
Unless otherwise specified, the term "heteroalkynyl" by itself or in combination with another term refers to a stable straight or branched chain alkynyl radical, or combination thereof, composed of a number of carbon atoms and at least one heteroatom or heteroatom group. In some embodiments, the heteroatom is selected from B, O, N and S, wherein the nitrogen and sulfur atoms are optionally oxidized and the nitrogen heteroatom is optionally quaternized. In other embodiments, the heteroatom group is selected from-C (═ O) O-, -C (═ O) -, -C (═ S) -, -S (═ O)2-、-C(=O)N(H)-、-N(H)-、-C(=NH)-、-S(=O)2N (h) -and-S (═ O) n (h) -. In some embodiments, the heteroalkynyl is C2-6A heteroalkynyl group; in other embodiments, the heteroalkyl is C2-4A heteroalkynyl group. The heteroatom or heteroatom group may be located at any internal position of the heteroalkynyl group, including the position of attachment of the alkynyl group to the rest of the molecule, but the terms "alkynyloxy", "alkynylamino" and "alkynylthio" are used conventionally to indicate attachment to the molecule via an oxygen atom, amino group or sulfur atom, respectivelyThose alkynyl groups of the remainder of the molecule. Examples of heteroalkynyl groups include, but are not limited to Up to two heteroatoms may be consecutive, e.g.
Unless otherwise specified, "cycloalkyl" includes any stable cyclic alkyl group including monocyclic, bicyclic, or tricyclic systems, wherein bicyclic and tricyclic systems include spirocyclic, bicyclic, and bridged rings. In some embodiments, the cycloalkyl is C3-8A cycloalkyl group; in other embodiments, the cycloalkyl is C3-6A cycloalkyl group; in other embodiments, the cycloalkyl is C5-6A cycloalkyl group. It may be mono-or poly-substituted, and may be mono-, di-or polyvalent. Examples of such cycloalkyl groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, norbornyl, [2.2.2 ] n]Bicyclo-octane, [4.4.0]Bicyclodecane and the like.
Unless otherwise specified, "cycloalkenyl" includes any stable cyclic alkenyl group containing one or more unsaturated carbon-carbon double bonds at any position in the group, including monocyclic, bicyclic, or tricyclic ring systems, including spirocyclic, bicyclic, and bridged rings, but any ring of such systems is non-aromatic. In some embodiments, the cycloalkenyl group is C3-8A cycloalkenyl group; in other embodiments, the cycloalkenyl group is C3-6A cycloalkenyl group; in other embodiments, the cycloalkenyl group is C5-6A cycloalkenyl group. It may be mono-or poly-substituted, and may be mono-, di-or polyvalent. Examples of such cycloalkenyl groups include, but are not limited to, cyclopentenyl, cyclohexenyl, and the like.
Unless otherwise specified, "cycloalkynyl" includes any stable cyclic alkynyl group containing one or more carbon-carbon triple bonds at any position in the group, including monocyclic, bicyclic, or tricyclic systems, including spirocyclic, bicyclic, and bridged rings. It may be mono-or poly-substituted, and may be mono-, di-or polyvalent.
Unless otherwise specified, the term "heterocycloalkyl" by itself or in combination with other terms denotes a cyclized "heteroalkyl," respectively, which includes monocyclic, bicyclic, and tricyclic systems, wherein bicyclic and tricyclic systems include spirocyclic, bicyclic, and bridged rings. Furthermore, with respect to the "heterocycloalkyl group," a heteroatom may occupy the position of the attachment of the heterocycloalkyl group to the rest of the molecule. In some embodiments, the heterocycloalkyl group is a 4-to 6-membered heterocycloalkyl group; in other embodiments, the heterocycloalkyl group is a 5-to 6-membered heterocycloalkyl group. Examples of heterocycloalkyl include, but are not limited to, azetidinyl, oxetanyl, thietanyl, pyrrolidinyl, pyrazolidinyl, imidazolidinyl, tetrahydrothienyl (including tetrahydrothien-2-yl and tetrahydrothien-3-yl, and the like), tetrahydrofuranyl (including tetrahydrofuran-2-yl, and the like), tetrahydropyranyl, piperidinyl (including 1-piperidinyl, 2-piperidinyl, and 3-piperidinyl, and the like), piperazinyl (including 1-piperazinyl and 2-piperazinyl, and the like), morpholinyl (including 3-morpholinyl and 4-morpholinyl, etc.), dioxanyl, dithianyl, isoxazolidinyl, isothiazolidinyl, 1, 2-oxazinyl, 1, 2-thiazinyl, hexahydropyridazinyl, homopiperazinyl, homopiperidinyl, or oxepanyl.
Unless otherwise specified, the term "heterocycloalkenyl" by itself or in combination with other terms denotes a cyclized "heteroalkenyl" group, respectively, which includes monocyclic, bicyclic, and tricyclic systems, including spiro, fused, and bridged rings, but any ring of such systems is non-aromatic. Furthermore, with respect to the "heterocycloalkenyl," a heteroatom may occupy both the heterocycloalkenyl and the rest of the moleculeA discrete connection location. In some embodiments, the heterocycloalkenyl is a 4-6 membered heterocycloalkenyl; in other embodiments, the heterocycloalkenyl is a 5-6 membered heterocycloalkenyl. Examples of heterocycloalkenyl include, but are not limited to
Unless otherwise specified, the term "heterocycloalkynyl" by itself or in combination with other terms denotes a cyclized "heteroalkynyl" group, respectively, which includes monocyclic, bicyclic, and tricyclic systems, including spiro, fused, and bridged rings. Furthermore, with respect to the "heterocycloalkynyl," a heteroatom may occupy the position of the heterocycle alkynyl attachment to the rest of the molecule. In some embodiments, the heterocycloalkynyl is a 4-to 6-membered heterocycloalkynyl; in other embodiments, the heterocycloalkynyl is a 5-to 6-membered heterocycloalkynyl. Unless otherwise specified, the term "halogen" or "halogen" by itself or as part of another substituent means a fluorine, chlorine, bromine or iodine atom. Furthermore, the term "haloalkyl" is intended to include monohaloalkyl and polyhaloalkyl. For example, the term "halo (C)1-C4) Alkyl "is intended to include, but not be limited to, trifluoromethyl, 2,2, 2-trifluoroethyl, 4-chlorobutyl, and 3-bromopropyl, and the like. Unless otherwise specified, examples of haloalkyl include, but are not limited to: trifluoromethyl, trichloromethyl, pentafluoroethyl, and pentachloroethyl.
"alkoxy" represents the above alkyl group having the specified number of carbon atoms attached through an oxygen bridge, unless otherwise specified, C1-6Alkoxy radicals comprising C1、C2、C3、C4、C5And C6Alkoxy group of (2). In some embodiments, the alkoxy is C1-3An alkoxy group. Examples of alkoxy groups include, but are not limited to: methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, sec-butoxy, n-butoxy,t-butoxy, n-pentyloxy, and S-pentyloxy.
Unless otherwise specified, the terms "aromatic ring" and "aryl" are used interchangeably herein and the terms "aromatic ring" or "aryl" refer to a polyunsaturated carbocyclic ring system which may be a monocyclic, bicyclic or polycyclic ring system in which at least one ring is aromatic, each of the rings in the bicyclic and polycyclic ring systems being fused together. Which may be mono-or poly-substituted, and may be mono-, di-or polyvalent, and in some embodiments the aryl group is C6-12An aryl group; in other embodiments, the aryl is C6-10And (4) an aryl group. Examples of aryl groups include, but are not limited to, phenyl, naphthyl (including 1-naphthyl and 2-naphthyl, and the like). The substituents of any of the above aryl ring systems are selected from the group of acceptable substituents described herein.
Unless otherwise specified, the terms "heteroaryl ring" and "heteroaryl" are used interchangeably herein, the term "heteroaryl" refers to an aryl (or aromatic ring) containing 1,2,3 or 4 heteroatoms independently selected from B, N, O and S, which may be a monocyclic, bicyclic or tricyclic ring system, wherein the nitrogen atom may be substituted or unsubstituted (i.e., N or NR, wherein R is H or other substituents already defined herein), and optionally quaternized, and the nitrogen and sulfur heteroatoms may be optionally oxidized (i.e., NO and S (O))pAnd p is 1 or 2). The heteroaryl group may be attached to the rest of the molecule through a heteroatom. In some embodiments, the heteroaryl is a 5-10 membered heteroaryl; in other embodiments, the heteroaryl is a 5-6 membered heteroaryl. Examples of such heteroaryl groups include, but are not limited to, pyrrolyl (including N-pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, and the like), pyrazolyl (including 2-pyrazolyl, 3-pyrazolyl, and the like), imidazolyl (including N-imidazolyl, 2-imidazolyl, 4-imidazolyl, 5-imidazolyl, and the like), oxazolyl (including 2-oxazolyl, 4-oxazolyl, 5-oxazolyl, and the like), triazolyl (1H-1,2, 3-triazolyl, 2H-1,2, 3-triazolyl, 1H-1,2, 4-triazolyl, and 4H-1,2, 4-triazolyl, and the like), tetrazolyl, isoxazolyl (3-isoxazolyl, 4-isoxazolyl, and 5-isoxazolyl, and the like), thiazolyl (including 2-thiazolyl, 5-isoxazolyl, and the like), thiazolyl (including the like), 4-thiazolyl and 5-thiazolyl, etc.), furyl (including 2-furyl and 3-furyl, etc.), thienyl (package)Including 2-thienyl and 3-thienyl, etc.), pyridyl (including 2-pyridyl, 3-pyridyl and 4-pyridyl, etc.), pyrazinyl, pyrimidinyl (including 2-pyrimidinyl and 4-pyrimidinyl, etc.), benzothiazolyl (including 5-benzothiazolyl, etc.), purinyl, benzimidazolyl (including 2-benzimidazolyl, etc.), indolyl (including 5-indolyl, etc.), isoquinolinyl (including 1-isoquinolinyl, 5-isoquinolinyl, and the like), quinoxalinyl (including 2-quinoxalinyl, 5-quinoxalinyl, and the like), quinolinyl (including 3-quinolinyl, 6-quinolinyl, and the like), pyrazinyl, purinyl, phenyloxazolyl, indazolyl (including 1H-indazolyl and 2H-indazolyl), and the like. The substituents of any of the above heteroaryl ring systems are selected from the group of acceptable substituents described herein.
Unless otherwise specified, the term "aralkyl" is intended to include those groups in which the aryl group is attached to an alkyl group, in some embodiments the aralkyl group is C6-10aryl-C1-4An alkyl group; in other embodiments, the aralkyl is C6-10aryl-C1-2An alkyl group. Examples of aralkyl groups include, but are not limited to, benzyl, phenethyl, naphthylmethyl, and the like. "aryloxy" and "arylthio" refer to those groups in which a carbon atom (e.g., methyl) in an aralkyl group has been replaced with an oxygen or sulfur atom, respectively, and in some embodiments, the aryloxy group is C6-10aryl-O-C1-2An alkyl group; in other embodiments, the aryloxy group is C6-10aryl-C1-2alkyl-O-. In some embodiments, the arylthio is C6-10aryl-S-C1-2An alkyl group; in other embodiments, the arylthio is C6-10aryl-C1-2alkyl-S-. Examples of aryloxy and arylthio groups include, but are not limited to, phenoxymethyl, 3- (1-naphthyloxy) propyl, phenylthiomethyl, and the like.
Unless otherwise specified, the term "heteroaralkyl" is intended to include those groups in which a heteroaryl group is attached to an alkyl group, in some embodiments the heteroaralkyl group is a 5-8 membered heteroaryl-C1-4An alkyl group; in other embodiments, the heteroaralkyl group is a 5-6 membered heteroaryl-C1-2An alkyl group. Examples of heteroaralkyl groups include, but are not limited to, pyrrolylmethyl, pyrazolylmethyl, pyridylmethyl, and the like,Pyrimidylmethyl and the like. "heteroaryloxy" and "heteroarylthio" refer to those groups in which a carbon atom (e.g., methyl) in a heteroarylalkyl group has been replaced by an oxygen or sulfur atom, respectively, and in some embodiments the heteroaryloxy group is a 5-8 membered heteroaryl-O-C1-2An alkyl group; in other embodiments, the heteroaryloxy group is a 5-6 membered heteroaryl-C1-2alkyl-O-. In some embodiments, the heteroarylthio is 5-8 membered heteroaryl-S-C1-2An alkyl group; in other embodiments, the heteroarylthio group is a 5-6 membered heteroaryl-C1-2alkyl-S-. Examples of heteroaryloxy and heteroarylthio include, but are not limited to, pyrrolooxymethyl, pyrazoloxymethyl, 2-pyridyloxymethyl, pyrrolothiomethyl, pyrazolthiomethyl, 2-pyridylthiomethyl and the like.
Unless otherwise specified, Cn-n+mOr Cn-Cn+mIncluding any one particular case of n to n + m carbons, e.g. C1-12Comprising C1、C2、C3、C4、C5、C6、C7、C8、C9、C10、C11And C12Also included are any ranges of n to n + m, e.g. C1-12Comprising C1-3、C1-6、C1-9、C3-6、C3-9、C3-12、C6-9、C6-12And C9-12Etc.; similarly, n to n + m means the number of atoms on the ring is n to n + m, for example, the 3-12 membered ring includes a 3-membered ring, a 4-membered ring, a 5-membered ring, a 6-membered ring, a 7-membered ring, an 8-membered ring, a 9-membered ring, a 10-membered ring, a 11-membered ring, and a 12-membered ring, and any range of n to n + m is also included, for example, the 3-12 membered ring includes a 3-6-membered ring, a 3-9-membered ring, a 5-6-membered ring, a 5-7-membered ring, a 6-8-membered ring, and a 6-.
The term "leaving group" refers to a functional group or atom that can be substituted by another functional group or atom through a substitution reaction (e.g., an affinity substitution reaction). For example, representative leaving groups include triflate; chlorine, bromine, iodine; sulfonate groups such as methanesulfonate, toluenesulfonate, p-bromobenzenesulfonate, p-toluenesulfonate and the like; acyloxy groups such as acetoxy, trifluoroacetyloxy, and the like.
The term "protecting group" includes, but is not limited to, "amino protecting group," hydroxyl protecting group, "or" thiol protecting group. The term "amino protecting group" refers to a protecting group suitable for use in preventing side reactions at the amino nitrogen position. Representative amino protecting groups include, but are not limited to: a formyl group; acyl, for example alkanoyl (such as acetyl, trichloroacetyl or trifluoroacetyl); alkoxycarbonyl such as tert-butoxycarbonyl (Boc); arylmethoxycarbonyl groups such as benzyloxycarbonyl (Cbz) and 9-fluorenylmethoxycarbonyl (Fmoc); arylmethyl groups such as benzyl (Bn), trityl (Tr), 1-bis- (4' -methoxyphenyl) methyl; silyl groups, such as Trimethylsilyl (TMS) and t-butyldimethylsilyl (TBS), and the like. The term "hydroxy protecting group" refers to a protecting group suitable for use in preventing side reactions of a hydroxy group. Representative hydroxy protecting groups include, but are not limited to: alkyl groups such as methyl, ethyl and tert-butyl; acyl groups, such as alkanoyl (e.g., acetyl); arylmethyl groups such as benzyl (Bn), p-methoxybenzyl (PMB), 9-fluorenylmethyl (Fm) and diphenylmethyl (benzhydryl, DPM); silyl groups, such as Trimethylsilyl (TMS) and t-butyldimethylsilyl (TBS), and the like.
The compounds of the present invention may be prepared by a variety of synthetic methods well known to those skilled in the art, including the specific embodiments listed below, embodiments formed by combinations thereof with other chemical synthetic methods, and equivalents thereof known to those skilled in the art, with preferred embodiments including, but not limited to, examples of the present invention.
The invention employs the following abbreviations: DCM represents dichloromethane; DMF represents N, N-dimethylformamide; DMSO represents dimethyl sulfoxide; EtOAc and EA represent ethyl acetate; MeOH represents methanol; BOC represents tert-butoxycarbonyl as an amine protecting group; THF represents tetrahydrofuran; boc2O represents di-tert-butyl dicarbonate; na (Na)2SO4Represents sodium sulfate; TEA for triethylamine; pd (dppf) Cl2Represents [1,1' -bis (diphenylphosphino) ferrocene]Palladium dichloride; NaOH represents sodium hydroxide; NaHMDS stands for sodium bis (trimethylsilyl) amide; HATU stands for 2- (7-azobenzotriazol)-N, N-tetramethyluronium hexafluorophosphate; DIEA stands for diisopropylethylamine; HCl represents hydrogen chloride; CDI represents N, N' -carbonyldiimidazole; MTBE represents methyl tert-butyl ether; pd2(dba)3Represents tris (dibenzylideneacetone) dipalladium; xanphos represents 4, 5-bis diphenylphosphine-9, 9-dimethylxanthene; PGE represents polyethylene glycol.
The compound is made by hand orThe software names, and the commercial compounds are under the supplier catalog name.
FIG. 1 shows the results of the drug effect of CT26 mouse syngeneic transplanted tumor model.
FIG. 2 shows the body weight change of mice in the CT26 mouse syngeneic transplantation tumor model.
The present invention is described in detail below by way of examples, but is not meant to be limited to any of the disadvantages of the present invention. The compounds of the present invention may be prepared by a variety of synthetic methods well known to those skilled in the art, including the specific embodiments listed below, embodiments formed by combinations thereof with other chemical synthetic methods, and equivalents thereof known to those skilled in the art, with preferred embodiments including, but not limited to, examples of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made in the specific embodiments of the invention without departing from the spirit and scope of the invention.
Example 1
Synthesis of Compound 1-b:
iodomethane (185.33g) was added to an acetone solution (1500mL) of Compound 1-a (98.5g) at 0 ℃ to react at 25 ℃ for 1 hour, followed by filtration and spin-drying of the filter cake to give Compound 1-b.
Synthesis of Compounds 1-d:
thionyl chloride (96.92g,814.69mmol) was slowly added dropwise to an anhydrous ethanol solution (1000mL) at 0 ℃ followed by addition of compound 1-c (95g, hydrochloride salt), reaction at 80 ℃ for 12 hours, and concentration under reduced pressure gave compound 1-d.
Synthesis of Compounds 1-e:
to a solution of compound 1-d (98g, hydrochloride) and N, N-diisopropylethylamine (141.01g,1.09mol) in dioxane (1400mL) and water (350mL) was added compound 1-b (208.74g,818.28mmol) in portions at 90 ℃, after 12 hours of reaction at 9008, the solvent dioxane was removed by concentration under reduced pressure, 1000mL of ethyl acetate was added, the solution was washed with saturated sodium chloride solution 3 times, 1000mL each time, the organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated to give compound 1-e.
Synthesis of Compounds 1-f:
to a solution of compound 1-e (76g) and N, N-bis (trifluoromethanesulfonyl) aniline (126.55g) in tetrahydrofuran (1500mL) was added NaHMDS (1M,371.09mL) dropwise at a temperature not higher than-60 ℃, reacted at 25 ℃ for 12 hours after completion of the dropwise addition, 1500mL of hydrochloric acid (0.2M) solution was added to quench the reaction, the solvent tetrahydrofuran was removed by concentration under reduced pressure, 1500mL of methyl t-butyl ether was added, the solution was washed with hydrochloric acid (0.2M) solution 2 times, 1500mL each time, 3 times with 5% NaOH solution, 1500mL each time, 1 time with saturated sodium chloride solution, 1500mL each time, the organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to give compound 1-f as a brown gum.
Synthesis of Compounds 1-h:
to a solution of compound 1-f (93.5g,261.65mmol) and pinacol boronate (73.09g,287.82mmol) in dioxane (1000mL) was added sodium bromide (10.77g,104.66mmol,3.37mL), potassium acetate (77.04g) and Pd (dppf) Cl2(9.57g) and after 12 hours of reaction at 80 ℃ under nitrogen,adding 2000 ml of ethyl acetate, filtering, washing the filtrate for 2 times with 1500ml of saturated sodium chloride solution each time, then adding 1000ml of hydrochloric acid solution (0.2M), separating to obtain an aqueous phase, extracting the organic phase twice with hydrochloric acid solution (0.2M) 1000ml each time, washing the combined aqueous phase for 2 times with ethyl acetate 2000 ml each time, then adjusting the pH to 9 with saturated sodium carbonate solution, extracting twice with ethyl acetate 1500ml each time, washing the combined organic phase extracted twice with saturated sodium chloride solution 2 times with 2000 ml each time, drying the organic phase with anhydrous sodium sulfate, filtering, and concentrating to obtain the compound 1-h.
Synthesis of Compounds 1-j:
to a solution of compound 1-h (29.85g) and 4-chloro-6-fluoroquinoline (14.7g) in dioxane (240mL) and water (60mL) was added triethylamine (24.57g) and Pd (dppf) Cl2(2.96g), after 12 hours of reaction at 100 ℃ under nitrogen, 1000mL of ethyl acetate was added, filtration was carried out, the filtrate was washed 2 times with saturated sodium chloride solution, 500mL each time, then the pH was adjusted to 1 with hydrochloric acid solution (1M), the aqueous phase was separated, the organic phase was extracted twice with hydrochloric acid solution (0.2M), 500mL each time, the combined aqueous phase was washed 2 times with ethyl acetate, 1000mL each time, then the pH was adjusted to 9 with saturated sodium carbonate solution, extracted twice with ethyl acetate, 750 mL each time, the combined organic phase was extracted twice with saturated sodium chloride solution, 2 times 1000mL each time, the organic phase was dried over anhydrous sodium sulfate, filtering, concentrating to obtain 30g of crude product, adding the crude product into 150 ml of acetonitrile, stirring for 1 hour at 25 ℃, filtering, and spin-drying a filter cake to obtain the compound 1-j.
Synthesis of Compounds 1-k:
to an isopropanol solution (400mL) of compound 1-j (21g) and pyridine (7.03g) was added palladium on carbon (4g, 10% purity), reacted at 50 ℃ under a hydrogen atmosphere (15psi) for 1 hour, and then filtered through celite, and concentrated to give compound 1-k.
Synthesis of Compounds 1-l:
after adding sodium hydroxide (6.73g) to a solution of compound 1-k (20g) in methanol (100mL), tetrahydrofuran (100mL) and water (50mL) and reacting at 70 ℃ for 12 hours, the solvents methanol and tetrahydrofuran were removed by concentration under reduced pressure, the pH was adjusted to 6-7 with hydrochloric acid (1M), a large amount of solid was precipitated, filtered, and the filter cake was dried under reduced pressure to give compound 1-l.
Synthesis of Compound 1:
adding N, N-diisopropylethylamine (5.90g) and HATU (8.11g) into a DMF solution (100mL) of compound 1-l (5g) and p-chlorophenylammonia (2.33g), reacting at 25 ℃ for 12 hours under the protection of nitrogen, adding 400mL of ethyl acetate for dilution, washing the solution with saturated sodium chloride solution for 3 times (500 mL each time), drying the organic phase with anhydrous sodium sulfate, filtering, concentrating to obtain 9g of crude product, adding the crude product into 45mL of acetonitrile, stirring for 12 hours at 25 acetonitrile, filtering, and spin-drying the filter cake to obtain the purified crude product, wherein the crude product is purified by preparative high-performance liquid chromatography (formic acid system) to obtain compound 1.
1H NMR(400MHz,DMSO-d6)=9.58(s,1H),8.82(d,J=4.5Hz,1H),8.08(dd,J=6.0,9.2Hz,1H),8.02(dd,J=2.8,11.2Hz,1H),,7.82-7.75(m,2H),7.65(dt,J=2.8,8.7Hz,1H),7.48(d,J=4.6Hz,1H),7.41-7.34(m,2H),3.39-3.34(m,1H),2.99(d,J=11.2Hz,2H),2.45-2.23(m,6H),1.91-1.68(m,6H).
Example 2
Synthesis of Compound 2
Compound 1-l (50mg) was dissolved in DMF (5mL) and DIEA (98.39mg) and HATU (86.84mg) were added to the solution at 25 ℃ and the resulting mixture was stirred at 25 ℃ for 30 minutes, then 2-a (23.59mg) was added to the reaction solution and the resulting mixture was stirred at 25 ℃ for 12 hours, the reaction was quenched by adding water (10mL) to the reaction solution and extracted with ethyl acetate (30 mL. about.3), the combined organic phases were Na2SO4Drying, filtering, and concentrating under reduced pressure to obtain crude product, and purifying the crude product by preparative chromatography to obtain the compound 2.
1H NMR(400MHz,CCl3D)ppm 1.69-1.91(m,3H)2.00-2.10(m,3H)2.33-2.56(m,6H)3.00(br d,J=11.74Hz,2H)3.18(br t,J=11.80Hz,1H)6.77-6.88(m,2H)7.31(d,J=4.65Hz,1H)7.39-7.48(m,1H)7.61(dd,J=10.33,2.63Hz,1H)8.03-8.15(m,1H)8.28(td,J=9.23,5.99Hz,1H)8.80(d,J=4.65Hz,1H)9.42(br s,1H).
Example 3
Synthesis of Compound 3
Compound 1-l (50mg) was dissolved in DMF (3mL) and DIEA (98.39mg) and HATU (86.84mg) were added to the solution at 25 ℃ and the resulting mixture was stirred at 25 ℃ for 30 minutes, then 3-a (23.31mg) was added to the reaction mixture and the resulting mixture was stirred at 25 ℃ for 12 hours, the reaction was quenched by adding water (10mL) to the reaction mixture and extracted with ethyl acetate (20 mL. sup.3), the combined organic phases were extracted with Na2SO4Drying, filtering, and concentrating under reduced pressure to obtain crude product, and purifying the crude product by preparative chromatography to obtain the compound 3.
1H NMR(400MHz,CCl3D)ppm 1.68-1.92(m,3H)1.96-2.10(m,3H)2.25-2.58(m,6H)3.02(br d,J=11.25Hz,2H)3.18(br t,J=12.10Hz,1H)7.01(dd,J=8.01,1.04Hz,1H)7.17(s,1H)7.21(s,1H)7.37-7.48(m,2H)7.60(dd,J=10.33,2.63Hz,1H)7.66(s,1H)8.07-8.14(m,1H)8.80(d,J=4.65Hz,1H)9.11(br s,1H).
Example 4
Synthesis of Compound 4
Compound 1-l (100mg) was dissolved in DMF (3mL), followed by addition of DIEA (118.07mg) and HATU (231.58 mg), and addition of 4-a (58.26mg) to the mixture, which was reacted at 25 ℃ N2The reaction solution is brown solution after 12 hours of reaction under protection. The reaction mixture was poured into 50mL of water, followed by extraction with 120mL (40 mL. times.3) of EA, and the combined organic phases were washed with 60mL (30 mL. times.2) of saturated brine, Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain 4.
1H NMR(400MHz,DMSO-d6)ppm 1.73-1.94(m,6H)2.24-2.45(m,7H)3.02(br d,J=11.2Hz,2H)4.14(s,3H)7.42(dd,J=9.2,1.6Hz,1H)7.49-7.57(m,2H)7.66(td,J=8.8,2.6Hz,1H)7.99-8.12(m,2H)8.26(s,1H)8.83(d,J=4.4Hz,1H)9.40(s,1H)
Example 5
Synthesis of Compound 5
Compound 1-l (100mg) was dissolved in DMF (5mL), DIEA (196.79mg), HATU (173.68mg),5-a (43.25mg) was added and nitrogen was substituted three times, the resulting mixture was stirred at 25 ℃ for 12 hours, the reaction was extracted with EtOAc (10mL 3), the combined organic phases were washed with saturated brine (10mL 3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 5.
1H NMR(400MHz,DMSO-d6)=9.67(s,1H),8.83(d,J=4.4Hz,1H),8.20(s,1H),8.11-8.01(m,2H),7.96(br dd,J=2.4,7.6Hz,1H),7.92(br dd,J=2.4,7.5Hz,1H),7.66(dt,J=2.8,8.7Hz,1H),7.58-7.48(m,1H),7.44-7.36(m,1H),3.56-3.28(m,1H),3.17(br s,2H),3.00(s,2H),2.97(br s,4H),2.54-2.53(m,4H),2.47-2.22(m,2H).
Example 6
Synthesis of Compound 6
Compound 1-l (100mg) was dissolved in DMF (5mL), followed by the addition of DIEA (196.79mg), HATU (173.68mg),6-a (33.22 mg). The reaction mixture was extracted with EtOAc (10 mL. times.3), the combined organic phases were washed with saturated brine (10 mL. times.3), and Na was added2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 6.
1H NMR(400MHz,DMSO-d6)=8.82(br d,J=3.8Hz,1H),8.19(s,1H),8.14-8.04(m,1H),8.01(s,1H),7.99(br s,1H),7.66(br t,J=7.6Hz,1H),7.47(br d,J=3.8Hz,1H),3.64(br s,1H),3.35-3.25(m,1H),2.90(br d,J=10.4Hz,2H),2.43-2.27(m,2H),2.16(br dd,J=8.8,19.4Hz,4H),1.92-1.80(m,2H),1.79-1.63(m,8H),1.58(br d,J=12.6Hz,1H),1.36-1.18(m,4H),1.14(br s,1H).
Example 7
Synthesis of Compound 7
Compound 1-l (50mg) was dissolved in DMF (5mL), DIEA (98.39mg), HATU (86.84mg), 7-a (28.81mg) was added and nitrogen was substituted three times, the resulting mixture was stirred at 25 ℃ for 12 hours, the reaction was extracted with EtOAc (10mL 3), and the combined organic phases were washed with saturated brine (10mL 3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain a compound 7.1H NMR(400MHz,DMSO-d6)=9.59(s,1H),8.83(d,J=4.4Hz,1H),8.31(br s,1H),8.12-8.07(m,2H),8.04(d,J=10.4Hz,2H),7.76-7.64(m,1H),7.54-7.47(m,3H),3.63(br s,1H),3.35(br t,J=11.2Hz,2H),2.99(br d,J=11.0Hz,2H),2.47-2.24(m,4H),1.92-1.68(m,6H).
Example 8
Synthesis of Compound 8
Compound 1-l (50mg) was dissolved in DMF (5mL), DIEA (98.39mg), HATU (86.84mg),8-a (18.61mg) was added and nitrogen was substituted three times, the resulting mixture was stirred at 25 ℃ for 12 hours, the reaction was extracted with EtOAc (10mL 3), and the combined organic phases were washed with saturated brine (10mL 3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 8.
1H NMR(400MHz,DMSO-d6)=9.52(s,1H),8.83(d,J=4.6Hz,1H),8.25(s,1H),8.12-8.01(m,2H),7.79-7.73(m,2H),7.67(dt,J=2.8,8.7Hz,1H),7.50(d,J=4.4Hz,1H),7.17(t,J=8.2Hz,2H),3.36(br t,J=12Hz,1H),3.26(br s,2H),3.00(br d,J=10Hz,2H),2.46-2.22(m,4H),1.93-1.68(m,6H).
Example 9
Synthesis of Compound 9
Compound 1-l (50mg) was dissolved in DMF (5mL), followed by addition of DIEA (98.39mg), HATU (86.84mg),9-a (18.11 mg). The reaction mixture was extracted with EtOAc (10 mL. times.3), the combined organic phases were washed with saturated brine (10 mL. times.3), and Na was added2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 9.
1H NMR(400MHz,DMSO-d6)=9.56(s,1H),8.83(d,J=4.4Hz,1H),8.76(d,J=2.2Hz,1H),8.22(s,1H),8.13-7.97(m,3H),7.66(dt,J=2.8,8.7Hz,1H),7.50(d,J=4.4Hz,1H),7.22(d,J=8.4Hz,1H),3.63(br s,1H),3.35(br t,J=11.2Hz,2H),3.10(br d,J=11.2Hz,4H),3.00(br d,J=11.2Hz,4H),2.46-2.24(m,2H),1.93-1.68(m,4H),1.65-1.42(m,2H)。
Example 10
Synthesis of Compound 10
Compound 1-l (62.68mg) was dissolved in DMF (2mL), followed by the addition of HATU (108.86mg) and DIEA (74.00mg) under a nitrogen atmosphere and the mixture was stirred at 25 ℃ for 30 minutes. 10-a (32.43mg) was then added. The mixture was stirred at 25 ℃ for 11.5 hours. The mixture remained pale yellow. The reaction was diluted with water (30mL) and extracted with EA (50mL × 2)The mixture was washed with saturated brine (30 mL. multidot.4) and Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified using preparative chromatography to afford compound 10.
1H NMR(400MHz,DMSO-d6)=10.13(br s,1H),8.92(d,J=4.4Hz,1H),8.23(dd,J=2.8,10.8Hz,1H),8.15(dd,J=5.6,9.2Hz,1H),7.75(dt,J=2.8,8.8Hz,1H),7.50-7.41(m,3H),7.39-7.33(m,1H),3.86-3.31(m,4H),2.79-2.65(m,4H),2.44-2.31(m,1H),2.28(s,3H),2.18-2.08(m,4H),2.04-1.84(m,2H)。
Example 11
Synthesis of Compound 11
Compound 1-l (54mg) was dissolved in DMF (1mL), followed by the addition of HATU (93.79mg) and DIEA (63.76 mg) under a nitrogen atmosphere. The mixture was stirred at 25 ℃ for 30 minutes. Thereafter, 11-a (28.72mg) was added. The mixture was stirred at 25 ℃ for 11.5 hours. The reaction was diluted with water (30mL) and extracted with EA (50mL × 2), the combined organic phases were washed with saturated brine (30mL × 4) and Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified using preparative chromatography to afford compound 11.
1H NMR(400MHz,DMSO-d6)=10.41(br s,1H),8.94(d,J=4.4Hz,1H),8.24-8.12(m,2H),8.07(dd,J=2.4,6.8Hz,1H),7.79-7.70(m,2H),7.54-7.46(m,2H),3.81(br s,1H),3.62(br d,J=11.2Hz,2H),3.28(br s,2H),2.84-2.61(m,4H),2.18-2.03(m,4H),2.00-1.77(m,2H)。
Example 12
Synthesis of Compound 12
Compound 1-l (62.68mg) was dissolved in DMF (1mL), followed by addition of HATU (108.86mg) and DIEA (74.00mg) under a nitrogen atmosphere. The mixture was stirred at 25 ℃ for 30 minutes. Thereafter, Compound 12-a (24.77mg) was added to the reaction solution. The mixture was stirred at 25 ℃ for 11.5 hours. The mixture was diluted with water (30mL) and extracted with EA (50mL × 2). The combined organic phases were washed with brine (30mL × 4), dried over Na2SO4, filtered and concentrated in vacuo to give the crude product. The crude product obtained is purified by preparative chromatography to afford compound 12.
1H NMR(400MHz,DMSO-d6)=9.69(s,1H),8.83(d,J=4.8Hz,1H),8.30(d,J=5.6Hz,1H),8.08(dd,J=6.0,9.2Hz,1H),8.03(dd,J=2.8,11.2Hz,1H),7.69-7.61(m,2H),7.59(dd,J=1.6,5.6Hz,1H),7.48(d,J=4.4Hz,1H),3.43-3.21(m,1H),2.98(br d,J=11.2Hz,2H),2.42(s,3H),2.41-2.35(m,2H),2.32(br d,J=8.4Hz,4H),1.91-1.68(m,6H)。
Example 13
Synthesis of Compound 13-b:
reactants 1-h (784.29mg,2.34mmol), reactant 13-a (500mg,2.13mmol), palladium catalyst (77.81mg,106.34 μmol), triethylamine (645.62mg,6.38mmol,888.07uL) were added to dioxane (8mL) and water (2mL) in this order, the reaction was heated to 90 ℃ under nitrogen protection and stirred for 12 hours, the reaction was filtered, the filtrate was diluted with water, extracted three times with ethyl acetate (50mL x 3), the organic phases were combined and washed three times with saturated brine (50mL x 3), dried over anhydrous sodium sulfate, filtered, the filtrate was concentrated to give a crude product, and purified by column chromatography to give compound 13-b.
Synthesis of Compound 13-c:
to ethyl acetate (10mL) was added reactant 13-b (750mg,2.06mmol), wet palladium on carbon (70mg, 10% purity) in that order, and the resulting mixture was replaced three times with hydrogen. Heating to 50 deg.C under hydrogen atmosphere and normal pressure, stirring for 2 hr, filtering the reaction solution, and concentrating the filtrate to obtain compound 13-c.
Synthesis of Compound 13-d:
to ethanol (8mL) and water (2mL) were added successively reactant 13-c (700mg,1.92mmol), sodium hydroxide (229.81mg,5.75mmol), heated to 70 ℃ and stirred for 2 hours, the reaction was concentrated, diluted with water, extracted three times with ethyl acetate (30mL x 3), the aqueous phase was added diluted hydrochloric acid (1M) to adjust pH to 3, extracted three times with ethyl acetate (30mL x 3), the resulting organic phases were combined and washed three times with saturated brine (50mL x 3), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated to give compound 13-d.
Synthesis of compound 13:
to DMF (10mL) was added reactant 13-d (500mg,1.48mmol), reactant 1-N (226.84mg,1.78mmol), condensing agent HATU (845.12mg,2.22mmol), N-diisopropylethylamine (574.51mg,4.45mmol,774.28uL) in this order, the reaction mixture was stirred at room temperature for 12 hours, the reaction mixture was diluted with ethyl acetate (30mL), washed three times with saturated ammonium chloride solution (30mL × 3), washed three times with saturated brine (30mL × 3), the organic phase was dried over anhydrous sodium sulfate, filtered, the filtrate was concentrated to give crude product, and purified by reverse phase chromatography to give compound 13.
1H NMR(400MHz,MeOD-d4)ppm 1.85-2.02(m,6H)2.40-2.55(m,6H)2.73-2.82(m,1H)3.12(s,3H)3.16(br d,J=11.49Hz,2H)7.36(d,J=7.78Hz,2H)7.57(d,J=8.31Hz,2H)7.65(d,J=8.46Hz,2H)7.91(d,J=8.44Hz,2H)8.13(s,1H)。
Example 14
Synthesis of Compound 14-a
To a solution of compound 1-l (1g) in DCM (30mL) was added CDI (740.68mg), reacted at 25 ℃ for 12 hours, diluted with 100mL DCM, washed with saturated brine (100mL × 3), and washed with Na2SO4Drying, filtering and concentrating to obtain the compound 14-a.
Synthesis of Compound 14
Compound 14-b (20.47mg) was dissolved in THF (5mL), then NaHMDS (1M,158.54uL) was added dropwise at 0 deg.C, nitrogen was replaced three times, and then allowed to react at 0 deg.CAnd (5) half an hour. Compound 14-a (50mg) was then added at 0 ℃. The resulting mixture was stirred at 0 ℃ for 1h, the reaction was extracted with EtOAc (10 mL. times.3), the combined organic phases were washed with saturated brine (10 mL. times.3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 14.
1H NMR(400MHz,DMSO-d6)=9.80(s,1H),8.83(d,J=4.4Hz,1H),8.45(s,1H),8.09(t,J=7.0Hz,2H),8.04(d,J=10.4Hz,4H),7.69-7.55(m,1H),2.98(br d,J=11.0Hz,2H),2.81-2.60(m,1H),2.57-2.53(m, 6H),2.44-2.37(m,4H),2.37-2.22(m,2H)。
Example 15
Synthesis of Compound 15
Compound 15-a (30.84mg) was dissolved in THF (2mL) after which NaHMDS (1M,190.25uL) was added dropwise at-75 ℃ under a nitrogen atmosphere over 10 mins. The mixture was stirred at-75 ℃ for 20 min. 14-a (60mg, 158.54. mu. mol) was then added. The mixture was stirred at 25 ℃ for 9.5 hours. The reaction was diluted with water (30mL) and extracted with EA (50mL × 2). The combined organic phases were washed with saturated brine (30 mL. multidot.4) and Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified using preparative chromatography to afford compound 15.
1H NMR(400MHz,DMSO-d6)=10.05(s,1H),9.10(d,J=2.4Hz,1H),8.82(d,J=4.4Hz,1H),8.48(dd,J=2.0,8.4Hz,1H),8.08(dd,J=6.0,9.2Hz,1H),8.02(dd,J=2.8,11.2Hz,1H),7.89(d,J=8.8Hz,1H),7.65(dt,J=2.8,8.8Hz,1H),7.48(d,J=4.4Hz,1H),3.01(br d,J=11.2Hz,2H),2.67(s,1H),2.47-2.29(m,6H),1.92-1.67(m,6H)。
Example 16
Synthesis of Compound 16
Compound 16-a (30.82mg) was dissolved in THF (2mL) after which NaHMDS (1M,190.25uL) was added dropwise at 0 ℃ over 10 minutes under a nitrogen atmosphere. The mixture was stirred at 25 ℃ for 20 minutes. 14-a (60mg) was then added. The mixture was stirred at 25 ℃ for 2 hours. The reaction was diluted with water (30mL) and extracted with EA (50mL × 2). The combined organic phases were washed with saturated brine (30mL 4), Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified using preparative chromatography to afford compound 16.
1H NMR(400MHz,DMSO-d6)=9.72(s,1H),8.83(d,J=4.4Hz,1H),8.31(s,1H),8.15(d,J=2.4Hz,1H),8.08(dd,J=5.6,9.2Hz,1H),8.03(dd,J=2.8,11.2Hz,1H),7.76(dd,J=2.4,8.8Hz,1H),7.65(dt,J=2.8,8.8Hz,1H),7.58(d,J=8.8Hz,1H),7.48(d,J=4.8Hz,1H),2.98(br d,J=11.2Hz,2H),2.67(br d,J=1.6Hz,1H),2.41(br t,J=10.4Hz,2H),2.35-2.25(m,4H),1.93-1.79(m,4H),1.79-1.67(m,2H)。
Example 17
Synthesis of Compound 17
Compound 17-a (20.23mg) was dissolved in THF (5mL), then NaHMDS (1M,158.54uL) was added dropwise at 0 ℃ three times with nitrogen substitution, then allowed to react at 0 ℃ for half an hour, then compound 14-a (50mg) was added at 0 ℃. The resulting mixture was stirred at 0 ℃ for 1h, the reaction was extracted with EtOAc (10 mL. times.3), the combined organic phases were washed with saturated brine (10 mL. times.3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 17.
1H NMR(400MHz,DMSO-d6)=9.94(s,1H),8.85(d,J=4.4Hz,1H),8.26(dd,J=1.4,8.2Hz,1H),8.11(t,J=7.0Hz,2H),8.05(d,J=10.4Hz,1H),7.68(dt,J=2.8,8.7Hz,1H),7.53(d,J=7.6Hz,1H),7.47(d,J=4.8Hz,1H),7.37(t,J=7.8Hz,1H),2.96(br d,J=11.4Hz,2H),2.69-2.53(m,1H),2.48-2.39(m,4H),2.39-2.31(m,2H),2.30-2.18(m,2H),2.01-1.72(m,4H)。
Example 18
Synthesis of Compound 18
Compound 18-a (25.55mg) was dissolved in THF (5mL), then NaHMDS (1M, 158.54. mu.L) was added dropwise at-78 deg.C, nitrogen replaced three times, and then allowed to react at-78 deg.C for half an hour. Compound 14-a (50mg) was then added at-78 ℃. The resulting mixture was stirred at 25 ℃ for 11.5 h, the reaction was extracted with EtOAc (10 mL. times.3), the combined organic phases were washed with saturated brine (10 mL. times.3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 18.
1H NMR(400MHz,DMSO-d6)=9.81(s,1H),8.83(d,J=4.5Hz,1H),8.12-7.93(m,4H),7.72-7.63(m,3H),7.49(d,J=4.5Hz,1H),3.62(br s,1H),3.35(br t,J=11.2Hz,2H),3.01(br d,J=11.1Hz,2H),2.47-2.25(m,4H),1.93-1.70(m,4H),1.68-1.47(m,2H)。
Example 19
Synthesis of Compound 19
Compound 19-a (18.73mg) was dissolved in THF (5mL) and NaHMDS (1M, 158.54. mu.L) was added dropwise at-78 ℃. The nitrogen was purged three times and then allowed to react at-78 ℃ for half an hour. Compound 14-a (50mg) was then added at-78 ℃. The resulting mixture was stirred at 25 ℃ for 11.5 h, the reaction was extracted with EtOAc (10 mL. times.3), and the combined organic phases were washed with saturated brine (10 mL. times.3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 19.
1H NMR(400MHz,DMSO-d6)=9.79(s,1H),8.84(d,J=4.4Hz,1H),8.28(s,2H),8.25(br s,3H),8.12-8.01(m,1H),7.66(dt,J=2.8,8.7Hz,3H),3.48-3.21(m,1H),3.00(br d,J=11.4Hz,2H),2.83-2.62(m,2H),2.57-2.53(m,4H),2.47-2.23(m,4H),1.93-1.69(m,2H).
Example 20
Synthesis of Compound 20
Compound 20-a (20.29mg) was dissolved in THF (2mL), NaHMDS (1M, 158.54. mu.L) was added dropwise at 0 deg.C, the mixture was stirred at 0 deg.C for 1 hour, and 14-a (50mg) was dissolved in THF (1mL) and added to the mixture, and the reaction was allowed to react at 0 deg.C for 1 hour under protection of N2. Water (15mL) was added to the reaction, followed by extraction with EA 45mL (15 mL. times.3), and the combined organic phases were washed with saturated brine (40mL, 20 mL. times.2), Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified by preparative chromatography to give product 20.
1H NMR(400MHz,DMSO-d6)ppm 1.72-1.91(m,6H)2.29-2.35(m,4H)2.43(br d,J=11.00Hz,2H)2.99(br d,J=10.88Hz,2H)3.35(br t,J=11.25Hz,1H)7.48(d,J=4.52Hz,1H)7.65(td,J=8.71,2.63Hz,1H)7.79(d,J=8.68Hz,2H)7.96-8.11(m,4H)8.82(d,J=4.52Hz,1H)9.87(s,1H)
Example 21
Synthesis of Compound 21-b
Mixture 21-a (500mg) and 1-h (1.02g) were dissolved in dioxane (12mL) and water (3mL), followed by the addition of TEA (836.08mg) followed by Pd (dppf) Cl2(201.52mg) in N2Adding into the mixture under protection, and mixing the mixture with N2The reaction was stirred at 85 ℃ for 12 hours to give a black solution, the reaction was filtered, 50mL of water was added, followed by extraction with EA (50 mL. times.3), and the combined organic phases were washed with 50mL (25 mL. times.2) of saturated brine, and N was addeda2SO4Drying, filtering and concentrating to obtain a crude product. The crude product is purified by column chromatography to obtain the compound 21-b.
Synthesis of Compound 21-c
Compound 21-b (590mg) was dissolved in EtOAc (10mL) and Pd/C (60mg, 10% pure) was added under protection of N2 and the resulting mixture was passed through H2And exchanging for several times, reacting at 25 deg.C and H2Stirring (15psi) for 12 hours to give a black suspension. Filtering and concentrating the reaction solution to obtain 21-c.
Synthesis of Compound 21-d
Compound 21-c (510mg) was dissolved in MeOH (8mL) and a solution of NaOH (228.95mg) in water (2mL) was added and the mixture was stirred at 75 deg.C for 12 h to give a yellow solution. The reaction was concentrated, then 6mL of water was added, the pH of the solution was adjusted to about 7 with 3M HCl, the solution was stirred at 0 ℃ for 30min, a large amount of white precipitate formed, filtered, and the filter cake was concentrated to 21-d.
Synthesis of Compound 21
Compound 21-d (160mg) was dissolved in DMF (4mL), followed by addition of DIEA (188.95mg) and HATU (240.88mg), the mixture was stirred at 25 ℃ for 1 hour, and 1-N (80.82mg) was added to the mixture, and the reaction was allowed to react at 50 ℃ for 11 hours under protection of N2 to give a yellow solution. Then 40mL of water was added to the reaction solution, followed by extraction with EA 60mL (20 mL. times.3), and the combined organic phases were washed with saturated brine 40mL (20 mL. times.2), Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified by preparative chromatography to afford compound 21.
1H NMR(400MHz,DMSO-d6)ppm 1.83-2.01(m,1H)1.83-2.01(m,2H)2.16(br s,4H)2.77(br s,4H)2.99-3.25(m,3H)3.61(br s,2H)7.53(br d,J=7.6Hz,2H)7.65(br s,1H)7.77-7.86(m,3H)8.79(br d, J=3.6Hz,1H)10.36(br s,1H)
Example 22
Synthesis of Compound 22-b
The mixture 22-a (500mg) and 1-h (980.66mg) were dissolved in dioxane (12mL) and water (3mL), followed by the addition of TEA (807.27mg) followed by Pd (dppf) Cl2(194.58mg) in N2Adding into the mixture under protection, and mixing the mixture with N2The reaction was stirred at 85 ℃ for 12 hours to obtain a brown solution, the reaction solution was filtered, 50mL of water was added, followed by extraction with EA150(50 mL. times.3), and the combined organic phases were washed with 50mL (25 mL. times.2) of saturated brine, Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product is purified by column chromatography to obtain the compound 22-b.
Synthesis of Compound 22-c
Compound 22-b (500mg) was dissolved in EtOAc (10mL) and Pd/C (50mg, 10% purity) was added under protection of N2, the resulting mixture was passed through H2 and permuted several times at 50 ℃ H2Stirring (15psi) for 3 hours to give a black suspension. The reaction solution is filtered and concentrated to obtain 22-c.
Synthesis of Compound 22-d
Compound 22-c (520mg) (crude) was dissolved in MeOH (10mL) and a solution of NaOH (228.62mg) in water (2mL) was added and the resulting mixture was stirred at 70 deg.C for 12 h to give a yellow solution. The reaction was concentrated, then 15mL of water was added, the pH of the solution was adjusted to about 6 with 2M HCl, and the mixture was concentrated to give the crude product. 15mL of MeOH was added to the crude product, stirred for 30min, filtered, and the filtrate was concentrated to 22-d.
Synthesis of Compound 22
Compound 22-d (200mg) was dissolved in DMF (5mL), followed by addition of DIEA (267.07mg) and HATU (340.48mg), and addition of 1-N (114.23mg) to the mixture, which was reacted at 25 ℃ with N2The reaction solution is yellow solution after 12 hours of reaction under protection. 40mL of water was added to the reaction, followed by extraction with EA 60(20 mL. times.3), and the combined organic phases were washed with 40mL (20 mL. times.2) of saturated brine, Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified by preparative chromatography to give compound 22.
1H NMR(400MHz,DMSO-d6)ppm 1.67-1.83(m,6H)2.13-2.35(m,7H)2.94(br d,J=11.2Hz,2H)3.82(s,3H)6.67(s,1H)6.90(d,J=5.2Hz,1H)7.37(d,J=8.80Hz,2H)7.73-7.78(m,2H)8.06(d,J=5.26Hz,1H)9.54(s,1H)。
Example 23
Synthesis of Compound 23-b
Compound 23-a (3.2g) was dissolved in EA (30mL), followed by addition of Me3OBF4(4.52g) under a nitrogen atmosphere. The mixture was stirred at 25 ℃ for 6 hours. The resulting suspension was filtered and the filter cake was dried in vacuo to give crude 23-b.
Synthesis of Compound 23-c
Compound 23-b (1.63g,10.86mmol) was dissolved in DCM (17mL), after which pyridine (1.29g) and iodobenzene bis (trifluoroacetic acid) (5.60g) were added under a nitrogen atmosphere. The resulting mixture was stirred at 30 ℃ for 30 minutes, after which I was added2(3.31 g). The resulting mixture was stirred at 30 ℃ for 15.5 hours. The resulting suspension was filtered and the filter cake was dried in vacuo to give crude 23-c.
Synthesis of Compound 23-d
Compounds 1-h (2.40g,7.17mmol,1eq) and 23-c (1.98g,7.17mmol,1eq) were dissolved in dioxane (20mL) and water (5mL), after which Pd (dppf) Cl was added2(262.41mg, 358.63. mu. mol,0.05eq) and Et3N (2.18g,21.52mmol,3.00mL,3eq) was placed under nitrogen. The mixture was replaced with nitrogen three times. The mixture was stirred at 100 ℃ for 8 hours. The mixture was filtered and the cake was washed with EA (80mL), followed by addition of water (40mL) and liquid separation. The aqueous phase was extracted with EA (70mL × 2). The combined organic phases were washed with saturated brine (50 mL. multidot.2), Na2SO4Drying, filtering and concentrating to obtain a crude product. The resulting crude product was purified using column chromatography to give compound 23-d.
Synthesis of Compound 23-e
Compound 23-d (800mg,2.24mmol) was dissolved in MeOH (10mL) before addition of Pd/C (80mg, 10% purity) in H2Atmosphere (35 psi). Replacing gas with the mixed solution for three timesBy H2. Adding the mixed solution in H2(15psi) the atmosphere was stirred at 50 ℃ for 20 hours. The mixture was filtered and the resulting solution was concentrated to give compound 23-e.
Synthesis of Compound 23-f
Compound 23-e (803mg,2.23mmol) was dissolved in MeOH (10mL) and water (5mL), after which NaOH (268.09mg,6.70mmol) was added under a nitrogen atmosphere. The resulting mixture was stirred at 70 ℃ for 12 hours. The solution was adjusted to pH 8 using 3M HCl, at which time a solid precipitated, filtered and the filter cake was washed with water (10mL) and dried in vacuo to give crude 23-f.
Synthesis of Compound 23
After compound 23-f (100mg, 301.76. mu. mol) was dissolved in DMF (2mL), DIEA (117.00mg, 905.29. mu. mol,157.68uL) and HATU (172.11mg, 452.65. mu. mol) were added under a nitrogen atmosphere. The resulting mixture was stirred at 25 ℃ for 30 min. Then 1-n (46.20mg, 362.12. mu. mol) was added. The mixture was stirred at 25 ℃ for 11.5 hours. The reaction was diluted with water (30mL) and extracted with EA (50mL × 2). The combined organic phases were washed with saturated brine (30mL 4) and Na2SO4Drying, filtering and concentrating to obtain a crude product. The crude product was purified by preparative chromatography to afford compound 23.
1H NMR(400MHz,DMSO-d6)=9.66(s,1H),7.79(d,J=8.8Hz,2H),7.65-7.53(m,2H),7.38(d,J=8.8Hz,2H),7.09(dt,J=2.4,9.2Hz,1H),4.09(s,3H),2.99(br d,J=11.2Hz,2H),2.67(s,1H),2.37-2.22(m,6H),2.19-2.04(m,2H),1.92-1.83(m,2H),1.79-1.69(m,2H).
Example 24
Synthesis of Compound 24
Compound 14-a (23.32mg) was dissolved in THF (3mL) and NaHMDS (1M, 158.54. mu.L) was added dropwise to the solution at-78 deg.C, the resulting mixture was stirred at-78 deg.C for 30 minutes, then 24-a (50mg, 132.12. mu. mol) dissolved in THF (3mL) was added dropwise to the reaction at that temperature, and the resulting mixture was stirred at 25 deg.C for 12 hours. The reaction was quenched by addition of water (10mL) and extracted with ethyl acetate (20mL x 3). the combined organic phases were dried over Na2SO4, filtered and concentrated under reduced pressure to give the crude product which was purified by preparative chromatography to give compound 24.
1H NMR(400MHz,DMSO-d6)ppm 1.67-1.97(m,6H)2.24-2.37(m,5H)2.42(br t,J=10.45Hz,2H)2.97(br d,J=11.37Hz,2H)7.49(d,J=4.52Hz,1H)7.67(td,J=8.71,2.63Hz,1H)7.78(dd,J=10.58,6.54Hz,2H)7.98-8.14(m,2H)8.84(d,J=4.52Hz,1H)9.80(s,1H).
Example 25
Synthesis of Compound 25
Compound 25-a (22.38mg) was dissolved in THF (3mL) and NaHMDS (1M,158.54 μ L) was added dropwise to the solution at-78 deg.C, the resulting mixture was stirred at-78 deg.C for 30 minutes, then 14-a (50mg) dissolved in THF (3mL) was added dropwise to the reaction at that temperature, the resulting mixture was stirred at 20 deg.C for 12 hours, water (10mL) was added to the reaction and the reaction was quenched and extracted with ethyl acetate (20mL x 3), the combined organic phases were Na2SO4Drying, filtering and concentrating under reduced pressure to obtain crude product, and purifying the crude product by preparative chromatography to obtain the compound 25.
1H NMR(400MHz,DMSO-d6)ppm 1.74-2.03(m,6H)2.21(br t,J=8.86Hz,2H)2.31-2.36(m,1H)2.34(br s,1H)2.33-2.46(m,1H)2.38-2.46(m,1H)2.38-2.44(m,1H)2.93(br d,J=11.00Hz,2H)3.92(s,3H)6.78(td,J=8.74,2.69Hz,1H)7.01(dd,J=10.76,2.69Hz,1H)7.50(d,J=4.52Hz,1H)7.63-7.74(m,1H)8.00-8.22(m,3H)8.88(d,J=4.52Hz,1H)9.71(s,1H)。
Example 26
Synthesis of Compound 26-a
To an ethanol solution (30mL) of compound 1-e (2g) was added sodium borohydride (503.80mg), and after 12 hours at 25 ℃, 100mL of ethyl acetate was added for dilution, the solution was washed three times with 100mL of saturated sodium chloride solution, and the organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated to give compound 26-a.
Synthesis of Compound 26-b
To a solution of compound 26-a (50mg) and 4-chloro-6-fluoroquinoline (39.95mg) in DMF (5mL) was added potassium tert-butoxide (74.05mg), reacted at 90 ℃ for 12 hours, diluted with 30mL of ethyl acetate, the solution washed 3 times with 30mL of saturated sodium chloride solution, and the organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated to give compound 26-b (300mg) as a yellow gum which was used directly in the next reaction.
Synthesis of Compound 26-c
To a solution of compound 26-b (300mg) in methanol (5mL) and water (5mL) was added sodium hydroxide (161.09mg), and after 12 hours at 65 ℃, the pH was adjusted to 6-7 with hydrochloric acid (1M), and the mixture was concentrated under reduced pressure to give a crude product, which was purified by a reverse phase flash column system to give compound 26-c.
Synthesis of Compound 26
To a solution of compound 26-c (40mg) and 1-N (16.30mg) in DMF (5mL) was added N, N-diisopropylethylamine ((75.06mg) and HATU (66.25mg), and after 12 hours of reaction under nitrogen at 25 ℃,30 mL of ethyl acetate was added for dilution, the solution was washed 3 times with saturated sodium chloride solution, 30mL each, and the organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated to give a crude product which was purified by preparative chromatography (formic acid system) to give 26.
1H NMR(400MHz,DMSO-d6)=9.56(s,1H),8.67(d,J=5.1Hz,1H),8.00(dd,J=5.4,9.2Hz,1H),7.77-7.60(m,4H),7.36(d,J=8.9Hz,2H),7.14(d,J=5.1Hz,1H),4.81(br s,1H),2.80-2.70(m,2H),2.50-2.40(m,2H),2.32-2.17(m,4H),2.15-2.05(m,2H),1.95-1.80(m,2H),1.80-1.57(m,2H).
Example 27
Synthesis of Compound 27-b
Compound 26-a (50mg), 27-a (39.93mg) was dissolved in DMF (5mL), followed by addition of t-BuOK (74.05mg) and nitrogen substitution three times, and the resulting mixture was stirred at 90 ℃ for 12 hours under nitrogen. The reaction was extracted with EtOAc (15 mL. times.3.) the combined organic phases were washed with saturated brine (15 mL. times.3), Na2SO4Drying, filtering and concentrating to obtain 27-b.
Synthesis of Compound 27-c
Compound 27-b (460mg) was dissolved in MeOH (5mL) and water (5mL), NaOH (148.22mg,3.71mmol) was added, the resulting mixture was stirred at 60 deg.C under nitrogen for 12 h, after the methanol was pulled dry, the pH was adjusted to 7 with 1M HCl at 0 deg.C, and the aqueous phase was spun dry directly. The crude product was purified by preparative chromatography to give compound 27-c.
Synthesis of Compound 27
Compound 27-c (50mg) was dissolved in DMF (5mL), DIEA (93.84mg, 726.05. mu. mol,126.47uL), HATU (82.82mg), p-chloroaniline (20.38mg) was added three times, nitrogen was substituted, the resulting mixture was stirred at 25 ℃ for 12 hours, the reaction was extracted with EtOAc (10 mL. about.3), the combined organic phases were washed with saturated brine (10 mL. about.3), Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by preparative chromatography to obtain the compound 27.
1H NMR(400MHz,DMSO-d6)=9.55(s,1H),8.53(d,J=5.6Hz,1H),7.75(d,J=8.0Hz,2H),7.44-7.34(m,1H),7.31-7.25(m,2H),7.29(dd,J=2.4,5.7Hz,1H),4.72(td,J=4.4,8.3Hz,1H),3.43(br s,2H),2.81-2.71 (m,4H),2.44-2.17(m,2H),2.12-1.94(m,2H),1.78-1.63(m,4H)。
Example 28
Synthesis of Compound 28-b
Compound 28-a (1g,6.04mmol, HCl) was dissolved in dioxane (8mL) and water (2mL) before DIEA (1.56g) was added. Then 1-b (2.31g,9.06 mmo) is addedl) to the mixture at 90 ℃. The resulting mixture was stirred at 90 ℃ for 12 hours. The mixture was extracted with EtOAc (10mL × 3), the combined organic phases were washed with saturated brine (10mL × 3), dried and Na was used2SO4Filtering, filtering and concentrating to obtain 28-b.
Synthesis of Compound 28-c
Compound 28-b (670mg,3.17mmol) was dissolved in THF (10mL), followed by addition of N, N-bis (trifluoromethanesulfonyl) aniline (1.25g,3.49 mmol). The mixture was replaced with nitrogen three times. NaHMDS (1M,4.12mL) was then added dropwise to the mixture at-78 ℃. The resulting mixture was stirred at-78 ℃ for 1 hour. The mixture was then stirred at 25 ℃ for 11 hours. The mixture was quenched by addition of 0.2M HCl (10mL), followed by extraction with MTBE (10mL _ 3), the combined organic phases were washed with 0.2M HCl (10mL _ 3), 0.5% NaOH (10mL _ 3) and saturated brine (10mL _ 3), dried over Na2SO4, filtered, and concentrated to give compound 28-c.
Synthesis of Compound 28-d
Compound 28-c (200mg, 582.55. mu. mol), bis-pinacolato borate (192.31mg, 757.31. mu. mol), KOAc (171.51mg,1.75mmol), NaBr (23.98mg, 233.02. mu. mol, 7.49. mu.L) were dissolved in dioxane (2mL), after which Pd (dppf) Cl was added under a nitrogen atmosphere2(21.31mg, 29.13. mu. mol). The mixture was replaced with nitrogen three times. The mixture was stirred at 100 ℃ for 12 hours. The reaction was filtered and washed with EA (80mL), after which water (40mL) was added. The organic phase obtained was washed with saturated brine (30mL _ 2). 1M HCl was used to adjust the pH of the solution to 3. The resulting aqueous phase was washed with EA (30mL × 2). The pH of the aqueous phase was adjusted to pH 10 using saturated sodium carbonate solution and extracted with EA (40mL × 2). The obtained organic phase was washed with saturated brine (20mL × 2), dried and used Na2SO4Filtered and concentrated to give crude 28-d.
Synthesis of Compound 28-e
Compound 28-d (210mg, 653.76. mu. mol) and 1-i (98.93mg, 544.80. mu. mol) were dissolved in dioxane (4mL) and water (1mL), followed by addition of Et3N (165.39mg,1.63mmol, 227.49. mu.L) and Pd (dppf) Cl2(19.93mg, 27.24. mu. mol) under nitrogen. Using nitrogen gas to the mixed solutionThe substitution was carried out three times. The mixture was stirred at 110 ℃ for 8 hours. The reaction mixture was filtered using EA (40mL), followed by washing with water (20 mL). The organic phase was washed with saturated brine (15mL × 2). 1M HCl was used to adjust the pH of the solution to 3. The aqueous phase was then washed with EA (15mL × 2). Saturated sodium carbonate solution was used to adjust the pH of the solution to pH 10 and EA (20mL × 2) was used for extraction. The obtained organic phase was washed with saturated brine (20mL × 2), dried and used Na2SO4Filtered and concentrated to 28-e.
Synthesis of Compound 28-f
Compound 28-e (156mg, 458.30. mu. mol) and pyridine (54.38mg, 687.44. mu. mol,55.49uL) were dissolved in IPA (2mL), after which Pd/C (32mg, 10% purity) in H was added2(15psi) atmosphere. The mixture was replaced three times with hydrogen. Adding the mixed solution in H2(15psi) the atmosphere was stirred at 50 ℃ for 8 hours. The mixture was filtered and the filtrate was concentrated to give compound 28-f.
Synthesis of Compound 28-g
Compound 28-f (152mg, 443.92. mu. mol) was dissolved in MeOH (2mL) and water (1mL), after which NaOH (71.03mg,1.78mmol) was added under a nitrogen atmosphere. The mixture was stirred at 70 ℃ for 12 hours. Concentrating the mixture to obtain crude product, and purifying by preparative chromatography to obtain 28-g.
Synthesis of Compound 28
Compound 28-g (26mg, 82.71. mu. mol) was dissolved in DMF (1mL) before HATU (47.17mg, 124.06. mu. mol) and DIEA (32.07mg, 248.13. mu. mol, 43.22. mu.L) were added under nitrogen. The mixture was stirred at 25 ℃ for 30 min. Then 1-n (12.66mg, 99.25. mu. mol) was added. The mixture was stirred at 25 ℃ for 11.5 hours. The mixture was diluted with water (30mL) and extracted with EA (50mL × 2). The combined organic phases were washed with saturated brine (30mL 4) and Na was used2SO4 was dried, filtered and concentrated in vacuo to give the crude product. The crude product was purified by preparative chromatography to afford compound 28.
1H NMR(400MHz,DMSO-d6)=10.11(s,1H),8.86(d,J=4.4Hz,1H),8.18-8.05(m,2H),7.98(dd,J=2.8,10.8Hz,1H),7.77(d,J=8.8Hz,2H),7.68(dt,J=2.8,8.8Hz,1H),7.58(d,J=4.4Hz,1H),7.39(d,J=8.8Hz,2H),3.29-3.25(m,1H),2.88(br d,J=11.1Hz,2H),2.54(br s,2H),2.09-2.01(m,2H),1.86(br d,J=12.0Hz,2H),1.15(br d,J=15.6Hz,4H).
Example 29
Synthesis of Compound 29
To DMF (5mL) was added successively reactant 1-l (50mg), reactant 29-a (34.72mg), HATU (86.84mg), diisopropylethylamine (59.04mg), and the reaction mixture was stirred at room temperature for 12 hours, ethyl acetate (30mL) was added to the reaction system, followed by washing three times with saturated ammonium chloride solution (30mL 3), washing three times with saturated brine (30mL 3), drying with anhydrous sodium sulfate, filtration, concentration of the filtrate to give a crude product, and purification of the crude product with a conventional reverse phase chromatography column to give compound 29.
1H NMR(400MHz,MeOD-d4)ppm 1.83-1.93(m,2H)1.94-2.01(m,2H)2.04(br s,2H)2.36-2.44 (m,2H)2.45-2.52(m,2H)2.54-2.61(m,2H)3.10-3.15(m,2H)3.36-3.39(m,1H)7.21(t,J=8.68Hz,1H)7.58(d,J=4.65Hz,1H)7.61(d,J=2.69Hz,1H)7.63(d,J=2.81Hz,1H)7.94(dd,J=10.70,2.63Hz,1H)8.07-8.08(m,1H)8.08-8.12(m,1H)8.79(d,J=4.65Hz,1H)。
Example 30
Synthesis of Compound 30
To DMF (5mL) was added successively reactant 1-l (50mg), reactant 30-a (28.80mg), HATU (86.84mg), diisopropylethylamine (59.04mg), and the reaction mixture was stirred at room temperature for 12 hours, ethyl acetate (30mL) was added to the reaction system, followed by washing three times with saturated ammonium chloride solution (30mL × 3), washing three times with saturated brine (30mL × 3), drying with anhydrous sodium sulfate, filtration, concentration of the filtrate to give a crude product, and purification of the crude product with a conventional reverse phase chromatography column to give compound 30.
1H NMR(400MHz,METHANOL-d4)ppm 1.92-2.00(m,2H)2.01-2.09(m,2H)2.09-2.16(m,2H)2.48-2.63(m,4H)2.71-2.83(m,2H)3.23-3.29(m,2H)3.48-3.53(m,1H)3.93(s,3H)7.21(dd,J=8.56,2.20Hz,1H)7.33(d,J=8.56Hz,1H)7.57(d,J=4.65Hz,1H)7.61(d,J=2.08Hz,1H)7.64(dd,J=8.74,2.14Hz,1H)7.96(dd,J=10.51,2.69Hz,1H)8.12(d,J=3.55Hz,1H)8.81(d,J=4.65Hz,1H)。
Example 31
Synthesis of Compound 31
The reaction product 14-a (23.08mg) was added to tetrahydrofuran (5mL), sodium hexamethyldisilazide (1M,264.24uL) was slowly added dropwise at-78 ℃, the reaction solution was stirred at that temperature for half an hour, then the reaction product 31-a (50mg) was added, the reaction solution was stirred at room temperature for 1 hour, ethyl acetate (30mL) was added to the reaction system, followed by washing three times with saturated ammonium chloride solution (30mL 3), then three times with saturated saline solution (30mL 3), drying with anhydrous sodium sulfate, filtration, concentration of the filtrate to give a crude product, and the crude product was purified by a conventional reverse phase chromatography column to give compound 31.
1H NMR(400MHz,MeOD-d4)ppm 1.84-1.95(m,2H)1.98(br d,J=12.72Hz,2H)2.04(br d,J=5.50Hz,2H)2.35-2.43(m,2H)2.46-2.60(m,4H)3.11(br d,J=10.88Hz,2H)3.37-3.47(m,1H)7.43(br s, 2H)7.56-7.65(m,2H)7.81(br d,J=11.86Hz,1H)7.93(br d,J=10.76Hz,1H)8.08-8.13(m,1H)8.77-8.81(m,1H)。
Example 32
Synthesis of Compound 32
To DMF (5mL) was added successively reactant 1-l (50mg), reactant 32-a (25.87mg), HATU (86.84mg), diisopropylethylamine (59.04mg), and the reaction mixture was stirred at room temperature for 12 hours, ethyl acetate (30mL) was added to the reaction system, followed by washing three times with saturated ammonium chloride solution (30mL × 3), washing three times with saturated brine (30mL × 3), drying with anhydrous sodium sulfate, filtration, concentration of the filtrate to give a crude product, and purification of the crude product with a conventional reverse phase chromatography column to give compound 32.
1H NMR(400MHz,DMSO-d6)ppm 1.81-1.99(m,2H)2.06-2.14(m,3H)2.28-2.42(m,5H)2.57-2.84(m,5H)2.86-2.93(m,2H)3.19-3.41(m,1H)7.42-7.50(m,2H)7.63(dd,J=8.50,2.26Hz,1H)7.69-7.77(m,2H)8.11-8.20(m,2H)8.91(d,J=4.52Hz,1H)10.15-10.28(m,1H)。
Example 33
Synthesis of Compound 33-b
After compound 33-a (2g,27.75mmol) was dissolved in MTBE (30mL), zinc iodide (88.59mg, 277.54. mu. mol) and TMSCN (2.75g,27.75mmol,3.47mL) were added and the resulting mixture was stirred at 25 ℃ for 12 hours and the reaction was concentrated to give crude compound 33-b.
Synthesis of Compound 33-c
After dissolving compound 33-b (3g,17.52mmol,1eq) and compound 33-h (3.26g,17.52mmol,1eq) in MTBE (50mL), the mixture was stirred at 25 ℃ for 12 hours and the reaction mixture was concentrated to give crude compound 33-c.
Synthesis of Compound 33-d
33-c (2g,7.48mmol) was dissolved in MeOH (20mL) and water (4mL) and NaOH (897.72mg,22.44mmol) was added, the resulting mixture was stirred at 60 ℃ for 4h, the reaction was concentrated and extracted with diluted EA (60 mL. times.2) with water (50mL), the aqueous phase was adjusted to pH 5 with HCl (1M,20mL), EA (100 mL. times.2) was extracted, the resulting organic phase was washed with saturated brine (100 mL. times.2), Na2SO4Drying, filtering and concentrating to obtain the compound 33-d.
Synthesis of Compound 33-f
Coupling 33-d (780mg,2.73mmol),33-e (782.20mg,3.28mmol), Pd2(dba)3(250.32mg,273.36μmol),Xantphos(316.34mg, 546.72. mu. mol) and cesium carbonate (1.78g,5.47mmol) were dissolved in 1,4 dioxane (20 mL). N for the resulting mixture2After three times of substitution, the reaction mixture was stirred at 80 ℃ for 12 hours, the reaction mixture was filtered, the filtrate was diluted with water (50mL), EA (80 mL. times.3) was extracted, the resulting mixed organic phase was washed with saturated brine (80 mL. times.2), and Na2SO4Drying, filtering and concentrating to obtain a crude product, and purifying the crude product by a silica gel plate to obtain the compound 33-f.
Synthesis of Compound 33-g
Compound 33-f (640mg,1.62mmol) was dissolved in MeOH (10mL) and HCl dioxane solution (4M,4.04mL) was added and the resulting mixture was stirred at 25 deg.C for 2h and the reaction was concentrated to give compound 33-g.
Synthesis of Compound 33
Compounds 33-g (200mg, 676.22. mu. mol),1-i (61.40mg, 338.11. mu. mol), Pd2(dba)3(61.92mg, 67.62. mu. mol), Xantphos (78.25mg, 135.24. mu. mol) and cesium carbonate (440.65mg,1.35mmol) were dissolved in 1,4 dioxane (10mL) and N was added2The substitution was carried out three times. The resulting mixture was stirred at 80 ℃ for 12 hours, the reaction was filtered, diluted with water (20mL), extracted with ethyl acetate (30 mL. times.3), and the resulting organic phase was washed with saturated brine (30 mL. times.2), washed with Na2SO4Drying, filtration and concentration followed by purification by preparative chromatography gave compound 33.
1H NMR(400MHz,MeOD-d4)ppm 2.95-3.04(m,4H)3.88-4.08(m,4H)4.86(br s,4H)7.29(d,J=6.85Hz,1H)7.34-7.38(m,2H)7.64-7.70(m,2H)7.79-7.97(m,2H)8.05(dd,J=9.29,4.89Hz,1H)8.58(d,J=6.85Hz,1H).
Example 34
Synthesis of Compound 34-a
Compound 23-f (100mg,301.76umol) was dissolved in DCM (5mL), CDI (73.40mg,452.65umol) was added to the solution, the resulting mixture was stirred at 25 ℃ for 12 hours, the reaction was quenched by addition of water (20mL) and extracted with ethyl acetate (20 mL. sup.3), the organics were combined and washed with water, and the mixture was concentrated and washed with waterThe phases were washed with saturated sodium chloride solution (20 mL. times.3), Na2SO4Drying, filtering and concentrating under reduced pressure to obtain 34-a.
Synthesis of Compound 34
Compound 20-a (29.73mg,251.67umol) was dissolved in THF (5mL) and NaHMDS (1M,251.67uL) was added dropwise to the solution at-78 deg.C, the resulting mixture was stirred at-78 deg.C for 30 minutes, then 34-a (80mg,209.73umol) dissolved in THF (2mL) was added dropwise to the reaction solution at that temperature, the resulting mixture was stirred at 20 deg.C for 12 hours, water (10mL) was added to the reaction solution to quench the reaction and extract with ethyl acetate (20mL x 3), the combined organic phases were Na2SO4Drying, filtering and concentrating under reduced pressure to obtain crude product, and purifying the crude product by preparative chromatography to obtain the compound 34.
1H NMR(400MHz,DMSO-d6)ppm 1.70-1.94(m,4H)2.03-2.21(m,2H)2.22-2.39(m,6H)2.99(br d,J=11.13Hz,2H)3.14(ddd,J=12.10,8.68,3.67Hz,1H)4.09(s,3H)7.09(td,J=9.26,2.38Hz,1H)7.52-7.65(m,2H)7.79(d,J=8.68Hz,2H)8.00(d,J=8.68Hz,2H)9.94(s,1H).
Experimental example 1: evaluation of Compounds on inhibition of the kynurenine pathway Activity of Hela cells
Purpose of the experiment:
the inhibitory effect of the compounds of the present invention on the kynurenine pathway activity of Hela cells was examined.
The experimental method comprises the following steps:
the compound was dissolved in dimethyl sulfoxide to 3mM, and 15. mu.l of the compound was added to a 96-well plate. Compounds (10. mu.l + 20. mu.l) were diluted 3-fold serially. 192. mu.l of detection medium was added to one 96-well plate, 78. mu.l of DMEM medium (Dulbecco's modified Eagle's medium) was added to the compound plate and mixed well, and 8. mu.l of the compound diluted with DMEM medium was transferred to the 96-well plate to which the detection medium had been added. The cell culture plate was removed and the medium was discarded, 100. mu.l of the compound was transferred to the cell culture plate, and the cell culture plate was transferred to an incubator for another 48 hours.
The cell culture plate was removed and 50. mu.l of the supernatant was transferred to a new 96-well plate, 200. mu.l of acetonitrile was added to the plate and mixed well, and 40. mu.l of the supernatant was transferred to another 96-well plate by 4-degree centrifugation. Add 360 u l containing 2u g/ml internal reference deionized water, fully mixing to detect the sample.
The experimental results are as follows: see table 1.
TABLE 1 in vitro screening test results
Test compounds | EC 50(nM) |
1 | 11 |
5 | 82 |
7 | 17 |
8 | 36 |
13 | 65 |
20 | 16 |
21 | 11 |
22 | 14 |
23 | 3 |
26 | 22 |
27 | 24 |
30 | 30 |
31 | 26 |
And (4) conclusion: the compound of the invention has obvious inhibition effect on the production of kynurenine in Hela cells.
Experimental example 2: evaluation of Compound pharmacokinetics
Test animal
Healthy adult female Balb/c mice used in this study were purchased from Shanghai Ling Biotech, Inc.
Preparation of drugs
Preparation of intravenous injection group administration solution
Accurately weighing a proper amount of compound, adding a certain amount of PEG400, performing ultrasonic treatment for 2 minutes to obtain a clear solution, and then adding a proper amount of pure water. A clear solution was obtained at a final concentration of 1mg/mL, with the dosing vehicle being 60% PEG400+ 40% pure water. The intravenous solution was filtered through a 2um filter before administration.
Preparation of oral administration solution
Accurately weighing a proper amount of compound, adding a certain amount of PEG400, performing ultrasonic treatment for 2 minutes to obtain a clear solution, and then adding a proper amount of pure water. A suspension solution with a final concentration of 10mg/mL was obtained, and the administration vehicle was 60% PEG400+ 40% pure water.
Administration of drugs
24 female Balb/c mice were divided into 4 groups of 6 mice each. Group one, 2mg/kg of BMS-986205 administered intravenously; second group, intragastric administration of 50mg/kg BMS-986205; third, compound 1 was administered at 2mg/kg intravenously; in the fourth group, 50mg/kg of Compound 1 was gavaged.
Sample collection
Blood was collected from three animals at each time point by a cross-bleed method. 40uL of whole blood was collected before and 0.0833 (intravenous group only), 0.25, 0.5, 1,2,4, 8, and 24 hours after administration, respectively. Whole blood was placed in an anticoagulation tube, centrifuged at 3000g for 15 minutes at 4 ℃ to prepare plasma and stored at-80 ℃. Drug concentrations in plasma were determined using LC/MS-MS.
TABLE 2 pharmacokinetic parameters
"-" indicates that no data has been obtained yet
And (4) conclusion: compared with the clinical molecule BMS-986205, the invention in example 1 has better tissue distribution, higher AUC under the same dosage, longer half-life, higher oral bioavailability and better comprehensive pharmacokinetic property.
Experimental example 3: evaluation of drug efficacy of compound and drug combination with PD-1 monoclonal antibody in CT26 mouse syngeneic transplantation tumor model
Test animals:
BALB/c mice, female, 18-22 g, Shanghai Slek.
The test drugs are:
example 1 and the control compound BMS-986205, PD-1 mab.
Cell and inoculation:
1. cell line: CT26 cell (mouse colon cancer cell)
2. Inoculation amount: 3X 105/0.1mL
3. The inoculation route is as follows: subcutaneous injection
Grouping and dosing regimens:
the experimental results are as follows: see fig. 1, fig. 2, and table 3.
TABLE 3 mouse tumor volume data in CT26 mouse syngeneic transplantation tumor model
a. The relative tumor proliferation rate T/C (%) ═ TRTV/CRTV × 100% (TRTV: mean RTV in the treatment group; CRTV: mean RTV in the negative control group).
b. Tumor growth inhibition rate TGI (%) -1- (average tumor volume on day of treatment group measurement-average tumor volume on day of treatment group measurement)/(average tumor volume on day of vehicle group measurement-average tumor volume on day of vehicle group measurement) × 100%
c.P value: day 18 tumor volume refers to comparison to vehicle control
And (4) conclusion: the compound shows excellent in-vivo anti-tumor activity and can play a synergistic role in PD-1 monoclonal antibody. Compared with the clinical molecule BMS-986205, the single drug and the combined drug effect are better under the same dosage.
Claims (13)
- A compound of formula (I) or a pharmaceutically acceptable salt thereof,wherein the content of the first and second substances,ring A is selected from phenyl, pyridyl, cyclopentyl, 2H-indazolyl, 1H-indolyl and cyclohexyl;ring B is selected from phenyl, pyridyl, quinolinyl, isoquinolinyl, 1H-indazolyl, and 2H-indazolyl;l is selected from a single bond, -O-, -CH2-、-CH2CH2-and-CH2CH2CH2-;X is selected from CH and N;l' is selected from a single bond and-O-;R1each independently selected from halogen and NH2、CN、C1-3Alkyl, CF3、CHF2、CH2F、C1-3A heteroalkyl group;R2each independently selected from halogen, CN, C1-3Alkyl, CF3、CHF2、CH2F、C1-3Heteroalkyl, 3-6 membered cycloalkyl, 3-6 membered heterocycloalkyl, 3-6 membered cycloalkyl-O-, 3-6 membered heterocycloalkyl-O-, 3-6 membered cycloalkyl-NH-, 3-6 membered heterocycloalkyl-NH-;m and n are each independently selected from 0, 1,2 and 3;"hetero" means a heteroatom or a heteroatom group, said C1-3"hetero" of heteroalkyl, 3-6 membered heterocycloalkyl-O-, and 3-6 membered heterocycloalkyl-NH-is selected from the group consisting of N, -NH-, -O-, -S-, -C (═ O) -, -C (═ S) -, -C (═ O) O-, -S (═ O) -, and-S (═ O)2-;In any of the above cases, the number of heteroatoms or heteroatom groups is independently selected from 1,2 and 3, respectively.
- A compound or pharmaceutically acceptable salt thereof according to claim 1, wherein R1Each independently selected from F, Cl, Br, I, NH2、CN、CH3、CF3、CH3CH2、CH3O and CH3CH2O。
- A pharmaceutical composition comprising a therapeutically effective amount of a compound according to any one of claims 1-11, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
- Use of a compound according to any one of claims 1 to 11, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition according to claim 12, for the manufacture of a medicament for the treatment of cancer.
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WO2017192813A1 (en) * | 2016-05-04 | 2017-11-09 | Bristol-Myers Squibb Company | Inhibitors of indoleamine 2,3-dioxygenase and methods of their use |
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CN107427499A (en) * | 2014-11-05 | 2017-12-01 | 弗莱塞斯生物科学公司 | Immunomodulator |
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