CN111865110B

CN111865110B - Electrophoresis power supply

Info

Publication number: CN111865110B
Application number: CN202010813560.4A
Authority: CN
Inventors: 谢蔚华
Original assignee: Guangzhou Daoyi Science And Technology Co ltd
Current assignee: Guangzhou Daoyi Science And Technology Co ltd
Priority date: 2020-08-13
Filing date: 2020-08-13
Publication date: 2022-04-19
Anticipated expiration: 2040-08-13
Also published as: CN111865110A

Abstract

The invention discloses an electrophoresis power supply which comprises an input end, a power inversion module, an output end, a monitoring module, a control module and an amplification module, wherein the input end is used for being connected with an external alternating current power supply; the control module is arranged to generate a voltage adjustment signal and a current adjustment signal according to the voltage data and the current data of the output end monitored by the monitoring module; the amplification module is configured to amplify the voltage adjustment signal and the current adjustment signal; the power inversion module is arranged to adjust the alternating current input by the input end into direct current according to the amplified voltage adjustment signal and the amplified current adjustment signal, and output the direct current through the output end after adjusting the voltage value and the current value of the direct current; the control module adopts a singlechip or a PLC. Because the amplifying module is arranged, the control signal is amplified by the amplifying module before being input into the power inverter module, and the adjustment precision of the power inverter module is improved; moreover, due to the arrangement of the monitoring module, the output voltage and current of the output end can be regulated and controlled in real time, so that the voltage and current of the output end can be accurately controlled.

Description

Electrophoresis power supply

Technical Field

The invention relates to the field of nucleic acid protein electrophoresis and transfer printing equipment, in particular to an electrophoresis power supply for nucleic acid protein electrophoresis and transfer printing equipment.

Background

The nucleic acid protein electrophoresis and transfer equipment is designed based on the principle that nucleic acid or protein has different motion speed in electric field due to different charges and molecular weights, and is used in qualitative or quantitative analysis of different nucleic acid or protein, or component analysis or single component extraction of different nucleic acid or protein in certain mixture.

However, there are devices in the market such as protein electrophoresis, protein transfer printing, nucleic acid electrophoresis, etc. at present, and the stability and precision of electric field control are relatively poor in the use performance.

Disclosure of Invention

In order to solve the problem that the electric field control stability and precision are relatively poor in the use performance of equipment such as general protein electrophoresis, protein transfer, nucleic acid electrophoresis and the like, according to one aspect of the invention, an electrophoresis power supply is provided.

The electrophoresis power supply comprises an input end, a power inversion module, an output end, a monitoring module, a control module and an amplification module, wherein the input end, the power inversion module, the output end, the monitoring module, the control module and the amplification module are used for being connected with an external alternating current power supply; the control module is arranged to generate a voltage adjustment signal and a current adjustment signal according to the voltage data and the current data of the output end monitored by the monitoring module; the amplification module is configured to amplify the voltage adjustment signal and the current adjustment signal; the power inversion module is arranged to adjust the alternating current input by the input end into direct current according to the amplified voltage adjustment signal and the amplified current adjustment signal, and output the direct current through the output end after adjusting the voltage value and the current value of the direct current; the control module adopts a singlechip or a PLC.

Because the amplifying module is arranged, the control signal is amplified by the amplifying module before being input into the power inverter module, and the adjustment precision of the power inverter module is improved; moreover, due to the arrangement of the monitoring module, the output voltage and current of the output end can be regulated and controlled in real time, so that the voltage and current of the output end can be accurately controlled.

Drawings

Fig. 1 is a schematic diagram of a module structure of an electrophoresis power supply according to a first embodiment of the present invention;

FIG. 2 is a schematic diagram of a module structure of an electrophoresis power supply according to a second embodiment of the present invention;

FIG. 3 is a schematic diagram of a module structure of an electrophoresis power supply according to a third embodiment of the present invention;

fig. 4 is a schematic circuit diagram of a protection module according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of a partial amplifying circuit of the protection module shown in FIG. 4;

FIG. 6 is a schematic diagram of another partial amplifying circuit of the protection module shown in FIG. 4;

FIG. 7 is a schematic diagram of a further partial amplifying circuit of the protection module shown in FIG. 4;

FIG. 8 is a schematic diagram of a partial amplifier circuit of the protection module shown in FIG. 4;

FIG. 9 is a schematic circuit diagram of a control module according to an embodiment of the present invention;

FIG. 10 is a circuit diagram of the integrated circuit of FIG. 9;

FIG. 11 is a schematic circuit diagram of the joint test workgroup of FIG. 9;

FIG. 12 is a circuit schematic of the memory device of FIG. 9;

FIG. 13 is a schematic circuit diagram of the low dropout voltage regulator shown in FIG. 9;

fig. 14 is a schematic circuit diagram of the switching regulator shown in fig. 9;

fig. 15 is a schematic circuit diagram of an amplifying module according to an embodiment of the present invention;

fig. 16 is a schematic circuit diagram of a digital-to-analog converter of the amplifying module shown in fig. 15;

FIG. 17 is a schematic diagram of a partial amplifier circuit of the amplifier module shown in FIG. 15;

FIG. 18 is a schematic diagram of another partial amplifier circuit of the amplifier module shown in FIG. 15;

fig. 19 is a schematic circuit diagram of a power inverter module connected to an input terminal, an output terminal, and a monitoring module according to an embodiment of the present invention;

fig. 20 is a schematic circuit diagram of the power inverter module shown in fig. 19 with the input terminals connected thereto;

fig. 21 is a schematic diagram of a partial amplifying circuit of the power inverter module shown in fig. 20;

fig. 22 is a schematic diagram of another partial amplifying circuit of the power inverter module shown in fig. 20;

fig. 23 is a schematic diagram of a further partial amplifying circuit of the power inverter module shown in fig. 20;

fig. 24 is a schematic circuit diagram of a monitoring module connected to an output terminal according to an embodiment of the present invention;

fig. 25 is a schematic diagram of a module structure of an electrophoresis power supply according to a fourth embodiment of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

Fig. 1 schematically shows an electrophoretic power supply according to a first embodiment of the present invention.

Referring to fig. 1, the electrophoresis power supply includes a control module 100, an amplification module 200, a power inversion module 300, an input terminal 400, an output terminal 500, and a monitoring module 600; wherein, the input terminal 400 is used for connecting with an alternating current power supply; the monitoring module 600 is configured to monitor data such as voltage data and current of the output terminal 500, and transmit the monitored voltage data and current data of the output terminal 500 to the control module 100; the control module 100 compares the received data of the voltage, the current and the like of the output end 500 with a preset value, generates a voltage adjusting signal and a current adjusting signal and outputs the voltage adjusting signal and the current adjusting signal to the amplifying module 200; the amplifying module 200 amplifies the received voltage adjusting signal and current adjusting signal and outputs the amplified signals to the power inverting module 300; the power inverter module 300 adjusts the ac power into the dc power according to the amplified voltage adjustment signal and current adjustment signal, and outputs the dc power through the output terminal 500 after adjusting the voltage and current values of the dc power.

Because the amplifying module 200 is arranged, the control signal is amplified by the amplifying module 200 before being input into the power inverter module 300, so that the adjustment precision of the power inverter module 300 is improved; moreover, due to the arrangement of the monitoring module 600, the output voltage and current of the output end 500 can be regulated and controlled in real time, so that the voltage and current of the output end 500 can be accurately controlled.

When the electrophoresis power supply is used, the output end 500 of the electrophoresis power supply is connected with equipment for protein electrophoresis, protein transfer printing, nucleic acid electrophoresis and the like, so that power can be supplied to the equipment for protein electrophoresis, protein transfer printing, nucleic acid electrophoresis and the like; because the monitoring module 600 is arranged to monitor the output voltage and current of the output end 500 in real time, the control module 100 and the monitoring module 600 can regulate and control the voltage and current of the output end 500 in real time through the power inverter module 300, so as to improve the adjustment speed of the electrophoresis power supply.

In some embodiments, the amplifying module 200 controls the power inverter module 300 to adjust the ac power to the dc power according to the compensation voltage, the voltage adjustment signal, and the current adjustment signal, and adjusts the values of the voltage and the current of the dc power and then outputs the adjusted values through the output terminal 500, so as to improve the accuracy of the power inverter module 300 in adjusting the voltage and the current; and since the amplifying module 200 amplifies the compensation voltage and outputs the amplified compensation voltage to the power inverter module 300, the power inverter module 300 can be applied to ac voltage input with a wide range of 110V-250V.

Referring to fig. 1, in some embodiments, the compensation voltage may also be fed back to the amplification module 200 directly from the power inverter module 300.

With continued reference to FIG. 1, in other embodiments, the compensation voltage is generated by the monitoring module 600 based on the monitored voltage and current data at the output 500; the amplification module 200 controls the power inverter module 300 to adjust the ac power input from the input terminal 400 to dc power according to the compensation voltage, and outputs the dc power through the output terminal 500 after adjusting the voltage value and the current value of the dc power.

Fig. 2 schematically shows an electrophoretic power supply according to a second embodiment of the invention.

Referring to fig. 2, the electrophoretic power supply further includes a protection module 700, and the protection module 700 is configured to compare the voltage data and the current data monitored by the monitoring module 600 with a preset limit value, and control the input terminal 400 or the output terminal 500 to be connected or disconnected with the power inverter module 300 according to the comparison result. For example, when at least one of the voltage data and the current data is greater than a preset limit value, the control input terminal 400 or the output terminal 500 is disconnected from the power inverter module 300; when the voltage data and the current data are both smaller than the preset limit value, the control input terminal 400 or the output terminal 500 is connected with the power inverter module 300 to realize the overload turn-off protection.

Referring to fig. 4 to 8, in some embodiments, the protection module 700 includes a dual voltage comparator integrated circuit of a type LM393 that compares the voltage value and the current value monitored by the monitoring module 600 with preset limit values and a buffer of a type 74ALS 05A; the buffer controls the power inverter module 300 to be connected or disconnected with at least one of the input terminal 400 and the output terminal 500 according to the comparison result; the buffer may directly control the power inverter module 300, or the control module 100 may control the power inverter module 300.

Further, referring to fig. 2, the comparison result may also be sent to the amplifying module 200, and the amplifying module 200 controls the power inverting module 300 to be connected to or disconnected from at least one of the input terminal 400 and the output terminal 500. The amplifying module 200 may also amplify the voltage value and the current value monitored by the monitoring module 600 and then input the amplified values to the protection module 700, so as to improve the control accuracy of the protection module 700.

In other embodiments, referring to fig. 7 and 8, the protection module 700 further includes a dual operational amplifier of the type LM358, and the voltage value and the current value monitored by the monitoring module 600 may be amplified by the dual operational amplifier and then output to the dual voltage comparator integrated circuit, so as to improve the comparison accuracy of the dual voltage comparator integrated circuit; the signals generated by the buffer can be amplified by the dual operational amplifiers and then output to the power inverter module 300, so that the precision of regulating the voltage and the current of the power inverter module 300 is improved; at this time, the protection module 700 may be directly connected to the monitoring module 600 and the power inverting module 300 without being connected to both through the amplifying module 200.

Referring to fig. 4 and 5, in some embodiments, the protection module 700 is also provided with a single channel inverter with schmitt trigger inputs, model NC7SZ14 or NC7SZ 04. Thus, the level signal of the protection module 700 can be matched to the level signal of the control module 100 by adapting the level through a single channel inverter with a schmitt trigger input.

With continued reference to fig. 4 and 5, in some embodiments, the protection module 700 is also provided with a monostable flip-flop model 74HC 123. To achieve pulse shaping by a monostable flip-flop. Therefore, the LM393 is used as a comparator to compare the output current and the overload current value for output control, and the buffer model 74ALS05A is subjected to forced turn-off protection (the amplification module 200 controls the power inverter module 300 to disconnect the input terminal 400 from the output terminal 500), and is fed back to the control module 100 through the monostable flip-flop model 74HC 123. When the control module 100 is connected to the display module, the monostable trigger is fed back to the information display of the control module 100 through the display module, so that a user can master the use state of the electrophoresis power supply in real time through the display module.

With continued reference to fig. 4 and 5, in some embodiments, the protection module 700 is also provided with a component of type MAX 6004. Referring to fig. 4 and 6, in some embodiments, the protection module 700 is also provided with an element of type IXDN 604. When the electrophoresis power supply is also provided with the fan, the rotating speed of the fan can be controlled so as to achieve the optimal heat dissipation speed.

Referring to fig. 2, in other embodiments, the protection module 700 outputs the comparison result to the control module 100, and controls the input terminal 400 or the output terminal 500 to be connected to or disconnected from the power inverter module 300 through the control module 100. The protection module 700 may directly output the comparison result to the control module 100; the protection module 700 may also be output to the control module 100 through the amplification module 200.

In some embodiments, the protection module 700 uses a temperature sensor of LM35DZ for high temperature detection protection, low voltage detection protection, leakage protection, etc. to implement the function of instant forced output shutdown.

Fig. 3 schematically shows an electrophoretic power supply according to a third embodiment of the invention.

Referring to fig. 3, the electrophoretic power supply further includes a display module 800, the display module 800 is connected to the control module 100, and the display module 800 is configured to display the voltage data and the current data received by the control module 100 from the monitoring module 600, so that a user can know the voltage information and the current information of the output terminal 500 in real time through the display module 800.

In some embodiments, the display module 800 is further configured to send at least one of a switch operating mode signal, a modify preset current value signal, a modify preset voltage value signal, a modify limit voltage value signal, and a modify limit current value signal to the control module 100.

The control module 100 is configured to switch, add or modify the operating mode based on the switch operating mode signal. For example, operation modes corresponding to a groove transfer device, a semi-dry transfer device, a nucleic acid gel electrophoresis device, and a protein gel electrophoresis device may be previously embedded in the control module 100, and a stepwise output mode previously embedded in the control module 100 may be invoked.

Modifying the preset current value according to the modified preset current value signal; and modifying the preset voltage value according to the modified preset voltage value signal. To accommodate different preparation schemes.

Modifying the limit voltage value according to the modified limit voltage value signal; the limit current value is modified based on the modified limit current value signal.

In some embodiments, the display module 800 can also switch the operating state of the control module 100 between on and off via an input signal.

In some embodiments, when a plurality of outputs 500 are provided, different operation modes may also be provided for each output 500 to perform different preparations simultaneously.

In some embodiments, the display module 800 is provided with an input unit and a display unit both electrically connected to the control module 100; wherein, the display unit is arranged to display the voltage data and the current data received by the control module 100 from the monitoring module 600, the working mode called by the input unit from the control module 100, and the virtual switch of the control module 100; the display unit is also configured to be able to display the limit current value and the limit voltage value; when a plurality of output terminals 500 are provided, each output terminal 500 correspondingly displays the display content. The input unit is configured to be able to control the module 100 to call up the working mode, and to be able to switch or add a new working mode by inputting the working mode switching information; the electrophoresis power supply is also set to be capable of switching the virtual switch between the on state and the off state by inputting a switch control signal so as to switch the control module 100 on and off and finally control the electrophoresis power supply to be switched on and off; it is also arranged to modify the current value and the voltage value preset by the control module 100, and modify the limit voltage value and the limit current value preset by the protection module 700.

Thus, a user may interact with the control module 100 through the display module 800.

In some embodiments, the display module 800 employs a liquid crystal screen.

In some embodiments, the display module 800 is developed as a UI interface program in DGUS using DMT48320M035_06WT available from denvin corporation, which is compatible with a resistive or capacitive lcd screen having a resolution of 480 × 320 inches and 3.5 inches from denvin corporation.

Regardless of the implementation manner, the control module 100, the amplifying module 200, the power inverting module 300, the input terminal 400, the output terminal 500 and the monitoring module 600 may be implemented by the following embodiments.

In some embodiments, the control module 100 employs a conventional single chip or PLC (Programmable Logic Controller).

Referring to fig. 9 and 10, the control module 100 includes an integrated circuit of model STM32F103R8T6 by which voltage and current adjustment signals are generated from the voltage and current data at the output 500 monitored by the monitoring module 600. For example, all packages of the single chip microcomputer which can be compatible with the program and is of the model STM32F103 are chips of the model LQFP 64.

Referring to fig. 9 and 11, the control module 100 further includes a joint test task group model JTAG-20 to program the single chip microcomputer.

Referring to fig. 9 and 12, the control module 100 further includes a memory device of model 24C02 to prevent the data of the single chip microcomputer from being lost due to power failure.

Referring to fig. 9 and 13, in some embodiments, control module 100 further includes a low dropout voltage regulator model LM1117 to provide current limiting and thermal protection.

Referring to fig. 9 and 14, in some embodiments, the control module 100 further includes a 3A output buck switching regulator, model LM2576-5.0, which can reduce the number of external components to a minimum and is easy to use due to its internal frequency compensator and a fixed frequency oscillator; and the efficiency of the three-stage linear voltage stabilizer is much higher than that of the popular three-stage linear voltage stabilizer, so that the three-stage linear voltage stabilizer is an ideal substitute.

The switching type buck regulator includes a 52kHz oscillator, a 1.23V reference voltage regulator circuit, a thermal shutdown circuit, a current limiting circuit, an amplifier, a comparator, an internal voltage regulator circuit, and the like. In order to generate different output voltages, the negative terminal of the comparator is usually connected to a reference voltage and the positive terminal is connected to a voltage dividing resistor network. The output of the output voltage divider resistance network is compared with the internal reference voltage-stabilizing value, if the voltage has deviation, the output duty ratio of the internal oscillator can be controlled by the amplifier, so that the output voltage is kept stable.

The low-dropout voltage regulator with the model number of LM1117 can provide 3.3V voltage for the singlechip.

In some embodiments, the input terminal 400 outputs +12V after passing through HS12P36SP to step down the output buck switching regulator model LM2576-5.0 to provide +5V to the overall control circuit.

Referring to fig. 9, in some embodiments, the control module 100 further includes a buzzer, which alarms when the control module 100 is disconnected from the power supply, so that the user knows that the control module 100 stops working.

With continued reference to fig. 9, in some embodiments, the control module 100 further includes a light emitting diode that emits light when the control module 100 is disconnected from the power source, so that the user can know that the control module 100 stops operating.

With continued reference to fig. 9, in some embodiments, the control module 100 further includes a dc power source to power the buzzer and the light emitting diode via the dc power source.

With continued reference to fig. 9, in some embodiments, the control module 100 further includes a bi-directional zener diode to achieve the voltage regulation function.

With continued reference to fig. 9, in some embodiments, a switch is further disposed on the control module 100 to control the start and stop of the control module 100 via the switch.

With continued reference to fig. 9, in some embodiments, a crystal oscillator is also provided on the control module 100 to generate a clock signal for the data processing device and to provide a reference signal for a particular system.

In some embodiments, the control module 100 controls the power on and off of the electrophoretic power supply by controlling the power on and off of the power inverter module 300. Specifically, the voltage adjusting signal and the current adjusting signal generated by the single chip microcomputer are amplified by the amplifying module and then control the power inverting module.

In some embodiments, the control module 100 is provided with a UART (Universal Asynchronous Receiver/Transmitter), the control module 100 is connected to the display module 800 through the UART, and specifically, the single chip is connected to the display module 800. Thus, different operation modes can be selected by the display module 800 to invoke different control programs in the control module 100.

In some embodiments, the control module 100 is provided with an SPI (Serial Peripheral Interface) and an IO (Input Output Interface) at the same time, and the single chip is connected to the monitoring module 600 and the amplifying module 200 through the SPI and IO interfaces. The monitoring module 600 transmits the monitored data such as voltage and current to the single chip microcomputer through the SPI interface and the IO interface to control and monitor output in real time.

In some embodiments, the single chip detects feedback signals of the monitoring module 600 and the protection module 700 through the SPI interface and the IO interface.

In some embodiments, a PID algorithm (proportional-integral-derivative algorithm) is embedded in the single chip microcomputer in advance. Therefore, the deviation between the current value and the voltage value actually output by the output terminal 500 and the set value is corrected through the PID algorithm, the output values of the current and the voltage of the output terminal 500 at the next time are adjusted through the power inverter module 300, and the data is amplified through the amplifying module 200, so that the current value and the voltage value adjusted through the power inverter module 300 are closer to the set value, and the stability and the precision of the electric field of the output terminal 500 are accurately controlled.

In some embodiments, a program can be embedded in a single chip microcomputer in advance, so that the preparation of multiple nucleic acid or protein electrophoretic transfer printing in a segmented mode can be realized. For example, a program can be embedded in the single chip microcomputer in advance, and a plurality of preparation schemes including a groove type or semi-dry type transfer preparation, a nucleic acid gel electrophoresis preparation, a protein gel electrophoresis preparation and the like can be set, and each scheme can be set in a sectional type.

Referring to fig. 15 and 17, in some embodiments, the amplifying module 200 includes a DAC (Digital to analog converter), and the control module 100 compares the data such as the voltage and the current sent by the monitoring module 600 with a set value to generate a voltage adjusting signal and a current adjusting signal, and converts the Digital signal into an analog signal through the DAC.

In some embodiments, the DAC adopts an external 12-bit DAC, the precision is 0.00061V, the control is effective and accurate, and the precision and the stability are improved.

With continued reference to fig. 15 and 17, in some embodiments, a DAC of model MAX5302 is employed.

Referring to fig. 15 and 16, in some embodiments, the amplifying module 200 is further provided with an amplifying circuit of a dual operational amplifier of a model LM358, and the amplifying module 200 amplifies the analog signal transmitted from the DAC and outputs the amplified analog signal to the power inverting module 300, so as to improve the accuracy of the power inverting module 300 in adjusting the output value of the output terminal 500. When the output current of the improved electrophoresis power supply is 10% -100% of the rated value and the output voltage is 50% -100%, the voltage stabilizing precision of the electric field is changed from 2% -3% to 1%; when the output voltage is 50% -100% of rated value and the output current is 20% -100% of rated value, the ripple factor is changed from 10% -1% to not more than 7.5-1%. Therefore, the DAC outputs 0-2.5V with the precision of 0.6mV under the control of the control module 100, and the DAC drives the amplifying circuit after the amplification of the double operational amplifiers and the sampling comparison of the output voltage.

Referring to fig. 15, 16 and 19, in some embodiments, the dual operational amplifier may further amplify the compensation voltage fed back by the power inverting module 300 or the compensation voltage transmitted by the power inverting module 300 through the output terminal 500 and the monitoring module 600, and then output the amplified compensation voltage to the power inverting module 300.

Referring to fig. 4, 15 and 19, in some embodiments, the dual operational amplifier may further amplify the comparison result output by the protection module 700 to the control module 100 and output the amplified comparison result to the protection module 700 or the power inverting module 300, so as to improve the control accuracy of the protection module 700.

With continued reference to fig. 15 and 18, in some embodiments, the amplification module 200 is further provided with a zener diode to obtain a stable voltage.

In some embodiments, the amplifying module 200 implements the automatic fine-tuning control of the power inverting module 300 according to the compensated voltage signal output by the monitoring module 600.

Referring to fig. 19 and 20, in some embodiments, the power inverter module 300 adjusts the ac power input from the input terminal 400 according to the voltage adjustment signal and the current adjustment signal amplified by the amplifying module 200 and outputs the adjusted ac power to the output terminal 500; the nucleic acid, protein electrophoresis and transfer apparatus is supplied with a power source having a stable voltage and current through an output terminal 500. The adjustment content comprises adjusting alternating current into direct current, and also comprises adjusting voltage and current of the direct current.

Referring to fig. 20, in some embodiments, the power inverter module 300 compensates for the direct current through a PFC circuit provided with an integrated circuit model MC34262D, a field effect transistor model IRFP22N50A or IRG4PC40F, a rectifier model MuR8100e, and a transformer model F133TS467001, so that the output voltage range is wide, so that the electrophoresis power supply can be simultaneously applied to electric field environments required for electrophoresis or transfer of different nucleic acids and proteins; the PFC circuit may further send the compensation voltage to the amplifying module 200, and the compensation voltage is amplified by the amplifying module 200, so as to improve the accuracy of the power inverting module 300 in adjusting the voltage and the current.

Referring to fig. 21, in some embodiments, the power inverter module 300 is provided with a high-speed PWM controller of model UC3825A, a MOSFET driver of model TC4427, and an output circuit of the inverter transformer of model F133TF220701, which controls the output of the inverter circuit, so that the circuit power of the electrophoresis power supply can meet the requirement.

In some embodiments, the power inverter module 300 processes the voltage adjustment signal, the current adjustment signal, and the compensation voltage signal by SPWM (Pulse Width Modulation, which changes the equivalent output voltage by changing the duty ratio of the output square wave; SPWM, which changes the Modulation Pulse mode based on PWM, and the Pulse Width time duty ratio is arranged according to the sine rule, so that the output waveform can be output as a sine wave by proper filtering, which is widely used in dc-ac inverters, etc.) and then outputs to the power inverter module 300; the SPWM signal adjusts the power supply AC and outputs the adjusted power supply AC to the output end 500. Therefore, the duty ratio of the power inverter module 300 can be adjusted through voltage feedback, and the purpose of stabilizing the output voltage is achieved.

Referring to fig. 22, in some embodiments, the power inverter module 300 rectifies the ac power received at the input terminal 400 into dc power through a rectifier circuit provided with a rectifier bridge of type GBU10K or MB05S, a triode of type S9013 or MMBT2222A, and a rectifier diode of type 10a 10. The maximum voltage of the direct current formed after rectification can reach 380V.

Referring to fig. 23, in some embodiments, the power inverting module 300 further inverts and rectifies the compensated dc power through an inverting full-wave rectification circuit provided with a fet of type W13NK100Z or IRFD120, a high-frequency transformer of type F1333ER404501, and a rectifier of type DSEI12-10A to obtain an output voltage of a single-phase full wave.

In some embodiments, the power inverter module 300 is further provided with an element model IXDN604, like a MOSFET driver model TC 4427.

In some embodiments, the alternating current is 110V-250V alternating current to adapt to different countries and regions.

In some embodiments, the ac power is 220V ac power, that is, the input end 400 is connected to the mains supply, so that the use is convenient and fast.

In some embodiments, the output terminal 500 employs a conventional power interface, so that the electrophoresis power supply can supply power to the protein electrophoresis, protein transfer, nucleic acid electrophoresis, and other devices through the power interface.

In some embodiments, the input 400 employs a conventional power interface, so that the electrophoretic power supply is connected to the mains power through the power interface.

Referring to fig. 24, in some embodiments, the monitoring module 600 performs output conversion by a conversion circuit provided with a sampling resistor and an operational amplifier of a model LM1211 or LT 1211; the output conditions of output voltage and current are detected through a dual-channel 12-bit serial analog-to-digital converter with the model number of MAX144, for example, the converted result of the conversion circuit is detected through the MAX 144; and fed back to the control module 100.

In some embodiments, the monitoring module 600 also monitors the output for overload by setting a monostable flip-flop model 74HC123 and a voltage comparator model LM 393.

In some embodiments, the detection chip MAX144 is connected to the control module 100 through an SPI interface and an IO interface.

In some embodiments, the protection module 700 receives the converted voltage of the monitoring module 600 sampled resistance.

Referring to fig. 15 and 19, in some embodiments, the operational amplifier of the monitoring module 600 is connected to the operational amplifier of the amplifying module 200 to amplify the compensation voltage transmitted from the power inverting module 300 and then control the power inverting module 300 more precisely.

In some embodiments, the protection module 700 continuously and slowly adjusts and increases the control output by monitoring the voltage and current feedback (i.e., the feedback of the MAX 144) of the module 600, so that the output of the power inverter module 300 is slowly increased to reach a set value, thereby implementing smooth start, reducing the start current, avoiding start overcurrent and overload, and implementing a soft start function; effectively reduces the impact and damage of output to equipment and nucleic acid and protein electrophoresis or transfer printing, prolongs the service life of the equipment and enhances the preparation effect of the nucleic acid and protein electrophoresis and the transfer printing.

In some embodiments, there are at least two outputs 500, and the control module 100 controls the output voltage and the output current of each output 500 individually.

In some embodiments, each output terminal 500 is individually connected to a set of the monitoring module 600, the power inverter module 300, and the amplifying module 200, and each of the monitoring module 600 and the amplifying module 200 is connected to the control module 100. For example, each output terminal 500 is individually connected with a set of sampling resistors, an operational amplifier with a model of LM1211 or LT1211, a detection chip MAX144, a monostable flip-flop with a model of 74HC123, a voltage comparator with a model of LM393, a high-speed PWM controller with a model of UC3825A, a MOSFET driver with a model of TC4427, an inverter transformer with a model of F133TF220701, a rectifier bridge with a model of GBU10K or MB05S, a triode with a model of S9013 or MMBT2222A, a rectifier diode with a model of 10A10, a field effect triode with a model of W13NK100Z or IRFD120, a high-frequency transformer with a model of F1333ER404501, a rectifier with a model of DSEI12-10A, a DAC with a model MAX5302, and a dual operational amplifier with a model LM 358.

Due to the arrangement of the operational amplifier and the transformer, the voltage and the current of the output end 500 can be ensured by arranging the two output ends 500.

In some embodiments, when the protection modules 700 are further provided, each output terminal 500 is separately connected with one protection module 700, each protection module 700 is separately connected with one monitoring module 600, and each protection module 700 is further separately connected with at least one of the power inverter module 300 and the control module 100. For example, each output 500 is also individually connected to a set of dual voltage comparator integrated circuits model LM393, a buffer model 74ALS05A, a dual op amp model LM358, a single channel inverter with schmitt trigger inputs model NC7SZ14 or NC7SZ04, a monostable flip-flop model 74HC123, and an element model MAX 6004.

Since the groove type transfer apparatus, the semi-dry type transfer apparatus, the nucleic acid gel electrophoresis apparatus, and the protein gel electrophoresis apparatus use different voltage and current conditions, a plurality of output terminals 500 are provided, and each output terminal 500 can be individually controlled by the control module 100. So that the electrophoresis power supply can simultaneously meet the preparation requirements of different conditions, for example, when two output terminals 500 are provided, one of the output terminals 500 is connected to the slot type transfer apparatus, and the other output terminal 500 is connected to the nucleic acid gel electrophoresis apparatus, the semi-dry type transfer apparatus, or the protein gel electrophoresis apparatus. Therefore, the electrophoresis power supply can simultaneously give consideration to the slot type transfer printing equipment, the semi-dry type transfer printing equipment, the nucleic acid gel electrophoresis equipment and the protein gel electrophoresis equipment.

In some embodiments, the output terminal 500 can be adjusted by a program embedded in the control module 100 to provide a power supply with a constant voltage of 1-300V, a constant current of 0.01-3A, and a constant power of 1-300W to the nucleic acid protein electrophoresis and transfer printing device, so as to meet the constant electric field requirements of various preparations such as tank-type or semi-dry-type transfer, nucleic acid gel electrophoresis, protein gel electrophoresis, and the like.

In some embodiments, a typical plant output 300V setting time is approximately one minute. By adopting the electrophoresis power supply, because the PID algorithm is embedded in the single chip microcomputer in advance, the single chip microcomputer can acquire the information of the monitoring output end 500 from the monitoring module 600 in real time, and output the control signal to the output end 500 through the amplifying module 200 and the power inversion module 300 so as to adjust the voltage and the current of the output end 500 in real time, thereby realizing high-efficiency adjustment output, and outputting 300V with the setting time of only 30 seconds.

Fig. 25 schematically shows an electrophoretic power supply according to a fourth embodiment of the present invention.

Referring to fig. 25, in some embodiments, the control module 100, the amplifying module 200, the power inverting module 300, the monitoring module 600 and the protection module 700 are integrated into a single body, for example, by the circuit board 30, in this case, the electrophoretic power supply further includes a housing 20, the circuit board 30 integrated with the control module 100, the amplifying module 200, the power inverting module 300, the monitoring module 600 and the protection module 700 is disposed in a receiving cavity of the housing 20, and the display module 800, the input terminal 400 and the output terminal 500 are disposed on the housing 20.

Referring to fig. 25, in some embodiments, to facilitate mounting of the circuit board 30, the housing 20 includes an upper case 21 and a lower case 22 that are detachably coupled.

In some embodiments, the enclosure 20 is sealed except at the bottom, has no water ingress, and has a distinct seal, effective for dust and water resistance.

Referring to fig. 25, in some embodiments, the housing 20 has an air inlet 23 at the bottom front end and a back heat sink fan 40 to provide good ventilation flow to allow the device to efficiently ventilate the fan 40.

In some embodiments, the bottom member of the housing 20 is an aluminum alloy, which provides good and reliable grounding and effective protection against static electricity.

What has been described above are merely some embodiments of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the inventive concept thereof, and these changes and modifications can be made without departing from the spirit and scope of the invention.

Claims

1. The electrophoresis power supply is characterized by comprising an input end, a power inversion module, an output end, a monitoring module, a control module and an amplification module, wherein the input end is used for being connected with an external alternating current power supply; wherein the content of the first and second substances,

the control module is arranged to compare the voltage data and the current data of the output end monitored by the monitoring module with a set value and then generate a voltage adjusting signal and a current adjusting signal;

the amplification module is configured to amplify the voltage adjustment signal and the current adjustment signal;

the power inversion module is arranged to adjust the alternating current input by the input end into direct current according to the amplified voltage adjustment signal and the amplified current adjustment signal, and output the direct current through the output end after adjusting the voltage value and the current value of the direct current;

the control module adopts a single chip microcomputer or a PLC;

the number of the output ends is at least two, and a group of monitoring module, a power inversion module and an amplification module are arranged corresponding to each output end;

each monitoring module and each amplifying module are connected with the control module;

the amplifying module also controls the power inverter module to adjust the alternating current input by the input end into direct current according to the compensation voltage, and the voltage value and the current value of the direct current are adjusted and then output through the output end;

a transformer is arranged in the power inversion module;

the amplifying module is provided with a double operational amplifier, the monitoring module is provided with an operational amplifier and is connected with the double operational amplifier of the amplifying module, so that the compensation voltage is transmitted to the power inversion module through the amplifying module after being amplified.

2. The electrophoresis power supply of claim 1, wherein a PID algorithm is pre-embedded in the single chip microcomputer, and the single chip microcomputer corrects the deviation of the voltage data and the current data of the output terminal from a set value through the PID algorithm.

3. The electrophoretic power supply according to claim 1, wherein the amplification module comprises a DAC and a dual operational amplifier; wherein the content of the first and second substances,

the DAC is arranged to convert the voltage adjustment signal and the current adjustment signal into analog signals, and the dual operational amplifier is arranged to amplify the analog signals and output the amplified analog signals to the power inverter module.

4. Electrophoresis power supply according to anyone of claims 1 to 3 further comprising a protection module configured to compare the voltage data and the current data monitored by the monitoring module with set limit values and to control the input terminal to be connected or disconnected with the power inversion module according to the comparison result.

5. The electrophoresis power supply of claim 4, wherein the voltage data and the current data monitored by the monitoring module received by the protection module are amplified by the amplification module; and/or

And the amplifying module amplifies the comparison result and outputs the amplified result to at least one of the power inverter module and the control module.

6. Electrophoresis power supply according to claim 5 wherein a set of protection modules is provided corresponding to each output, each protection module being individually connected to one monitoring module and at least one of the power inverter module and the control module.

7. Electrophoretic power supply according to claim 6, further comprising a display module arranged to display the voltage data and the current data monitored by the monitoring module as received by the control module.

8. The electrophoretic power supply according to claim 7, wherein the display module is further configured to send at least one of a switch operating mode signal, a modify preset current value signal, a modify preset voltage value signal, a modify limit voltage value signal, and a modify limit current value signal to the control module;

the control module is set to switch, newly add or modify the working mode according to the working mode signal, modify the preset current value and the preset voltage value according to the modified preset current value signal and the modified preset voltage value signal, and modify the limit voltage value and the limit current value according to the modified limit voltage value signal and the modified limit current value signal.

9. Electrophoretic power supply according to claim 8, wherein the display module is further arranged to enable different operation modes to be set for different outputs by the control module.

10. The electrophoresis power supply of claim 9 wherein the control module is provided with a UART, and the control module is connected to the display module via the UART to select different operation modes via the display module to invoke different control programs in the control module.

11. The electrophoresis power supply of claim 8, wherein the power inversion module, the monitoring module, the control module, the protection module, and the amplification module are integrated on a circuit board.

12. The electrophoretic power supply of claim 11, further comprising a housing;

the circuit board integrated with the power inverter module, the monitoring module, the control module, the protection module and the amplification module is arranged in the accommodating cavity of the shell;

the display module, the input end and the output end are arranged on the surface of the shell;

the display module is arranged on the shell, and the display module is arranged on the shell.