CN111838674A

CN111838674A - Composition with anti-sugar effect and application thereof

Info

Publication number: CN111838674A
Application number: CN202010749801.3A
Authority: CN
Inventors: 李春美; 杜霞; 李凯凯; 朱琳
Original assignee: Huazhong Agricultural University
Current assignee: Huazhong Agricultural University
Priority date: 2020-07-30
Filing date: 2020-07-30
Publication date: 2020-10-30

Abstract

The invention discloses a composition with an anti-sugar effect and application thereof, wherein the composition comprises the following raw materials in parts by weight: 5-10 parts of mulberry extract, 5-10 parts of raspberry extract, 15-20 parts of strawberry extract, 5-10 parts of roselle extract, 15-20 parts of lycium ruthenicum extract, 5-10 parts of persimmon tannin cracking product, 35-50 parts of oligomeric inulin and 1-5 parts of citric acid; the composition is used for preparing medicines or health-care foods for treating, delaying or relieving ACEs related diseases; the composition can obviously inhibit the formation of AGEs, simultaneously shows extremely strong free radical scavenging capacity, can be gradually popularized to the fields of cosmetics, foods and medicines, and has wide future prospect.

Description

Composition with anti-sugar effect and application thereof

Technical Field

The invention relates to the technical field of health-care food, in particular to a composition with an anti-sugar effect and application thereof.

Background

Advanced glycation end products (AGEs) can be divided into endogenous AGEs and exogenous AGEs. Endogenous AGEs are produced by reaction of proteins in organisms and redundant saccharides, and particularly have the highest content in blood and cells of patients with renal failure and diabetes, so that physiological changes such as diabetes, renal failure and the like are influenced, and human health is seriously influenced. Exogenous AGEs refer to AGEs taken in by organisms from the outside, mostly carried in by food, also called food-borne AGEs.

As the AGEs can change the structure and the function of protein, the accumulation of the AGEs has great relation with diseases such as neuropathy, Alzheimer syndrome, atherosclerosis and the like and can cause diabetic complications such as diabetic nephropathy, retinopathy and the like, and in addition, the AGEs can be combined with a specific receptor of a cell membrane and cause the pathological changes of tissues and organs by abnormally activating a signal path such as NF-kappa B, MAPK and the like. At present, AGEs inhibitors such as aspirin and aminoguanidine used in medical clinic all have certain toxic and side effects. Therefore, there is a need to develop novel AGEs inhibitors that are free of adverse effects.

Disclosure of Invention

In view of the problems of the prior art, the invention aims to provide a composition with anti-saccharification effect and application thereof.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides a composition with an anti-sugar effect, which comprises the following raw materials in parts by weight: 5-10 parts of mulberry extract, 5-10 parts of raspberry extract, 15-20 parts of strawberry extract, 5-10 parts of roselle extract, 15-20 parts of lycium ruthenicum extract, 5-10 parts of persimmon tannin cracking product, 35-50 parts of oligomeric inulin and 1-5 parts of citric acid. The main component of the fruit extract is anthocyanin which is extremely unstable in aqueous solution, so in order to improve the stability of the anthocyanin and increase the activity of the anthocyanin for inhibiting AGEs, the invention adds oligomer procyanidin as a stabilizer and a synergist of the anthocyanin.

As a further improvement of the invention, the content of Cy-3-glc and Cy-3-rut2 main anthocyanins in the mulberry extract is not less than 47.5% and 20.5%; the content of Cy-3-soph and Cy-3-glc 2 major anthocyanins in the red raspberry extract is not less than 30% and 37.5%; the Pg-3-glc content in the strawberry extract is not less than 60%; the content of 3 kinds of anthocyanin Dp-3-sam, Dp-3-glc and Cy-3-glc in the Hibiscus sabdariffa extract is not less than 8.5%, 14.5% and 10%; the content of petunidin-3-rutinoside in the Lycium ruthenicum Murr extract is not less than 60%.

As a further improvement of the invention, the persimmon tannin cracking product is persimmon procyanidin oligomer obtained by carrying out high-pressure catalytic hydrogenolysis on persimmon tannin, the average polymerization degree is not more than 3, and the content of A-ECG and EGCG dimer is not less than 10%.

As a further improvement of the invention, the polymerization degree of the oligoinulin is not more than 10.

As a further improvement of the invention, the preparation methods of the mulberry extract, the raspberry extract, the strawberry extract, the roselle extract and the lycium ruthenicum extract are as follows: respectively weighing and grinding the mulberries, the red raspberries, the strawberries, the roselle and the lycium ruthenicum mill, extracting by adopting hydrochloric acid methanol, enriching by AB-8 macroporous resin, eluting, and preparing by medium pressure chromatography to obtain a crude extract rich in anthocyanin.

The mulberry extract, the raspberry extract, the strawberry extract, the roselle extract and the lycium ruthenicum extract are mainly anthocyanin crude extracts, and the specific preparation method comprises the following steps: weighing Mori fructus, Rubi fructus, strawberry, calyx Hibisci Sabdariffae and fructus Lycii respectively, grinding, adding 0.1% hydrochloric acid-containing methanol at a ratio of material to liquid (m/m) of 1:2, placing in dark place, performing ultrasound treatment at 40 deg.C for 30min, filtering the extractive solution with gauze, performing ultrasound treatment on the filter residue under the same conditions for 3 times, mixing the extractive solutions, and removing methanol with rotary evaporator under vacuum condition at 45 deg.C. Filtering the anthocyanin concentrated solution with Buchner funnel, adjusting pH to 1.5, loading into chromatographic column filled with AB-8 resin material, adsorbing for 60min, removing impurities such as protein and sugar with 0.1% volume fraction hydrochloric acid distilled water, detecting eluate with phenol-sulfuric acid method to colorless, eluting anthocyanin with 60% ethanol with pH of 2.5, collecting eluate, and removing ethanol by rotary evaporation at 45 deg.C under vacuum condition. Extracting the concentrated solution with ethyl acetate at a ratio of 1:1(v/v), repeatedly extracting for 3 times under the same conditions, combining water phases, vacuum concentrating, freeze-drying, and further purifying by medium-pressure preparative chromatography to obtain crude anthocyanin extract.

The invention also provides application of the composition with the anti-sugar effect in preparing medicines or health-care foods for treating, delaying or relieving ACEs related diseases.

As a further improvement of the invention, the AGEs-related diseases are diabetes, atherosclerosis, chronic kidney disease, liver disease or neurodegenerative disease.

As a further improvement of the invention, the medicine also comprises pharmaceutically acceptable auxiliary materials, and the health food also comprises the auxiliary materials which are acceptable in food science.

As a further improvement of the invention, the medicament is in the form of oral preparation or injection preparation.

As a further improvement of the invention, the oral preparation is tablets, powder, capsules, granules, pills, powder, paste or oral liquid.

The invention discloses the following technical effects:

the invention provides a composition with the anti-sugar effect, which takes a plurality of fruit extracts rich in anthocyanin as raw materials, fully utilizes the synergistic effect of the anthocyanin and the oligomer, and adds oligoinulin which can improve the taste and has stable anthocyanin and procyanidin and citric acid which can improve the taste on the basis of the synergistic effect of the anthocyanin and the oligomer. The composition with the anti-sugar effect can obviously inhibit the formation of AGEs and simultaneously shows extremely strong free radical scavenging capacity. In summary, all experiments demonstrated that the composition had a significant effect of inhibiting the formation of AGEs. The raw materials related by the invention are all food raw materials, have small side effect and high safety, can be applied to human bodies, can be gradually popularized to the fields of cosmetics, foods and medicines, and have wide future prospects.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

FIG. 1 is a high performance liquid chromatogram of anthocyanin content in a mulberry extract, wherein A is a high performance liquid chromatogram of the mulberry extract at a wavelength of 280nm, and B is a high performance liquid chromatogram of the mulberry extract at a wavelength of 520 nm;

FIG. 2 is a high performance liquid chromatogram of anthocyanin content in a raspberry extract, wherein A is a high performance liquid chromatogram of a raspberry extract at a wavelength of 280nm, and B is a high performance liquid chromatogram of a raspberry extract at a wavelength of 520 nm;

FIG. 3 is a high performance liquid chromatogram of the anthocyanin content of a strawberry extract, wherein A is the high performance liquid chromatogram of the strawberry extract at a wavelength of 280nm, and B is the high performance liquid chromatogram of the strawberry extract at a wavelength of 520 nm;

FIG. 4 is a high performance liquid chromatogram of the anthocyanin content of Hibiscus sabdariffa extract, wherein A is the high performance liquid chromatogram of the Hibiscus sabdariffa extract at a wavelength of 280nm, and B is the high performance liquid chromatogram of the Hibiscus sabdariffa extract at a wavelength of 520 nm;

FIG. 5 is a high performance liquid chromatogram of the anthocyanin content of Lycium ruthenicum Murr extract, wherein A is a high performance liquid chromatogram of Lycium ruthenicum Murr extract at a wavelength of 280nm, and B is a high performance liquid chromatogram of Lycium ruthenicum Murr extract at a wavelength of 520 nm;

FIG. 6 is a graph of the amelioration of high AGEs feed toxicity by a preferred composition of the present invention, wherein A is a graph of the significant inhibition of the activation of the RAGE-p38MAPK-NF- κ B signaling pathway by the preferred composition of the present invention, and B is a graph of the significant inhibition of the expression of TNF- α and IL-6 inflammatory factors in the liver by the preferred composition of the present invention.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

EXAMPLE 1 preparation of crude anthocyanin extract

Respectively taking 50g of mulberry, raspberry, strawberry, roselle and lycium ruthenicum, grinding, adding 100ml of methanol containing 0.1% hydrochloric acid at a material-liquid ratio (m/m) of 1:2, placing in a dark place, carrying out ultrasonic treatment at 40 ℃ for 30min, filtering the extracting solution with gauze, repeatedly carrying out ultrasonic treatment on filter residues for 3 times under the same condition, combining the extracting solutions, and removing the methanol by using a rotary evaporator under the vacuum condition of 45 ℃. Filtering the anthocyanin concentrated solution with Buchner funnel, adjusting pH to 1.5, loading into chromatographic column filled with AB-8 resin material, adsorbing for 60min, removing impurities such as protein and sugar with 0.1% volume fraction hydrochloric acid distilled water, detecting eluate with phenol-sulfuric acid method to colorless, eluting anthocyanin with 60% ethanol with pH of 2.5, collecting eluate, and removing ethanol by rotary evaporation at 45 deg.C under vacuum condition. Extracting the concentrated solution with ethyl acetate at a ratio of 1:1(v/v), repeating the extraction for 3 times under the same conditions, combining the water phases, vacuum concentrating, and freeze drying to obtain 5 kinds of crude anthocyanin extracts.

HPLC detection analysis of 5 kinds of anthocyanin crude extracts

1mg of Cy-3-glc standard substance is quantitatively weighed and dissolved in HCl methanol solution with volume fraction of 0.1% to prepare 1mg/ml Cy-3-glc standard solution, and then the standard solution is filtered by a 0.45 mu m filter membrane into a brown liquid phase bottle for liquid phase analysis. The liquid chromatography conditions were as follows: a Waters C18 column (250 mm. times.4.6 mm,5 μm) was used; mobile phase A: 5% formic acid water, mobile phase B: methanol; gradient elution conditions: 0-10 min, 5% -20% of B; 10min to 15min, 20 percent to 20 percent of B; 15 min-30 min, 20% -25% B; 30-35 min, 25-25% B; 35-40 min, 25-33% B; 40 min-42 min, 33% -5% B; 42min to 47min, 5 percent to 5 percent of B; the flow rate is 1mL/min, the detection wavelength is 520nm by a PDA detector, the sample introduction amount of the standard substance is 1, 2, 4, 8 and 16 mu L, the sample introduction is repeated for 3 times in each volume, and the average peak area of each sample introduction volume is calculated. The mass (. mu.g) was plotted on the abscissa and the peak area on the ordinate, giving a standard curve of y 2000000x-42033 (R)²＝0.9999)。

Detection of anthocyanin content in 5 kinds of anthocyanin crude extracts

Respectively taking 5 anthocyanin crude extracts, quantitatively dissolving with HCl methanol containing 0.1% of volume fraction, taking a proper amount of dissolving solution, filtering with a filter membrane of 0.45 mu m, transferring into a brown liquid phase bottle, and analyzing the liquid phase conditions in the same HPLC detection of the 5 anthocyanin crude extracts, wherein the detection wavelengths are 280nm and 520 nm. And substituting the obtained peak area into the standard curve for quantitative calculation.

Separating 5 kinds of anthocyanin crude extract with MPPSS

300mg of 5 kinds of crude extracts of anthocyanin are respectively taken and ultrasonically dissolved by 2mL of analytically pure methanol and filtered by a filter membrane with the diameter of 0.45 mu m for standby. The gradient elution conditions of the crude extracts of the anthocyanin of the mulberries and the red raspberries are as follows: 0-2 min, 20% B; 2-22 min, 20% -30% of B; 22-32 min, 30-40% of B, and the gradient elution conditions of the crude extracts of strawberry and roselle anthocyanin are as follows: 0-2 min, 20% B; 2-22 min, 20-30% of B, 22-32 min, 30-40% of B, 32-47 min and 40-45% of B, wherein the elution conditions of the lycium ruthenicum anthocyanin crude extract are as follows: 0-2 min, 20% B; 2-22 min, 20% -30% of B; 22-32 min, 30% -40% B; 32-47 min, 40-45% B; 47-57 min, 45-60% of B, a detection wavelength of 280nm, a preparation column model Flash C18(80g, 20-35 μm, 100A), 2 columns connected in series, and a sample is subjected to peak cutting and collection, wherein the collection unit is 10 mL.

In the MPSS separation process, research shows that anthocyanin is stable under an acidic condition, tailing conditions can be effectively reduced and the separation effect can be well improved by adding formic acid, the flow rate is 40mL/min, the A mobile phase is 2% formic acid water, and the B mobile phase is methanol.

Determining content of main anthocyanin in the crude extract

Respectively mixing freeze-dried samples of different anthocyanin components obtained by MPSSs separation according to the same proportion, dissolving a certain amount of the freeze-dried samples by using 0.1% HCl methanol, filtering the mixture by using a 0.45 mu m filter membrane, performing liquid phase analysis, performing HPLC detection analysis on 5 kinds of anthocyanin crude extracts under the same liquid phase condition, injecting 10 mu L of the sample, and detecting the wavelengths of 280nm and 520 nm.

Structural identification of the obtained anthocyanin fraction by UPLC-MS

Chromatographic column

BEH Waters C18(2.1 mm. times.100 mm,1.7 μm); the sample volume is 2 mu L; the column temperature is 45 ℃; the flow rate is 0.4 mL/min; mobile phase A: 0.1% formic acid water, mobile phase B: methanol; elution conditions: 0-0.5 min, 2% -2% B; 0.5-1 min, 2% -5% of B; 1-2 min, 5% -10% of B; 2-5 min, 10% -20% of B; 5-8 min, 20% -100% B; 8-11 min, 100% B; 11-11.5 min, 100% -5% B; 11.5-13 min, 5% -5% B. Electrospray ionization ion source, scanning particle range: 50-1000 m/z, positive ion mode, atomizer pressure of 40psi, gas flow rate of 10L/min, dryer temperature of 350 ℃, capillary voltage of 3500V.

The detection result of the high performance liquid chromatography of anthocyanin content in the mulberry extract is shown in figure 1, wherein A is the high performance liquid chromatogram of the mulberry extract at the wavelength of 280nm, and B is the high performance liquid chromatogram of the mulberry extract at the wavelength of 520 nm. As can be seen from fig. 1A and 1B, there are 2 main peaks and fewer peaks at both wavelengths, and the content of 2 main anthocyanins in mulberry was calculated to be 47.5% and 20.5% by using cyanidin-3-glucoside (Cy-3-glc) as a standard.

The high performance liquid chromatography detection result of anthocyanin content in the red raspberry extract is shown in fig. 2, wherein A is the high performance liquid chromatogram of the red raspberry extract at the wavelength of 280nm, and B is the high performance liquid chromatogram of the red raspberry extract at the wavelength of 520 nm. As can be seen from FIG. 2, the crude extract of raspberry anthocyanin has 2 main peaks at 520nm, and many impurity peaks before the 2 major peaks of anthocyanin at 280 nm. Taking Cy-3-glc as a standard substance, calculating the contents of 2 main anthocyanins in the crude extract of raspberry anthocyanin to be 30% and 37.5% respectively.

The detection result of the strawberry extract anthocyanin content by high performance liquid chromatography is shown in figure 3, wherein A is a high performance liquid chromatogram of the strawberry extract at a wavelength of 280nm, and B is a high performance liquid chromatogram of the strawberry extract at a wavelength of 520 nm. As can be seen from FIG. 3, there are 1 major peak at 520nm wavelength, indicating that the strawberry flower extract mainly contains 1 anthocyanin, while at 280nm wavelength, there are more impurities before and after the major anthocyanin peak, and the content of 1 major anthocyanin in the strawberry anthocyanin crude extract is 60% using Cy-3-glc as the standard.

The high performance liquid chromatography detection result of anthocyanin content in Hibiscus sabdariffa extract is shown in figure 4, wherein A is high performance liquid chromatogram of Hibiscus sabdariffa extract at wavelength of 280nm, and B is high performance liquid chromatogram of Hibiscus sabdariffa extract at wavelength of 520 nm. As can be seen from FIG. 4, the crude Hibiscus sabdariffa anthocyanin contains 3 major anthocyanins at 520nm, and contains some impurities before the major peaks of 3 anthocyanins at 280nm, and the content of 3 anthocyanins in Hibiscus sabdariffa is calculated to be 8.5%, 14.5% and 10% respectively, using Cy-3-glc as standard.

The high performance liquid chromatography detection result of anthocyanin content in Lycium ruthenicum Murr extract is shown in FIG. 5, wherein A is a high performance liquid chromatogram of Lycium ruthenicum Murr extract at a wavelength of 280nm, and B is a high performance liquid chromatogram of Lycium ruthenicum Murr extract at a wavelength of 520 nm. As can be seen from FIG. 5, there are only 1 main peak at 520nm wavelength, and 280nm liquid phase diagram shows that many non-anthocyanin substances are present before the main peak of anthocyanin, and the content of 1 main anthocyanin contained in Lycium ruthenicum is calculated to be 60% by taking Cy-3-glc as a standard.

Example 2

The composition with the anti-sugar effect comprises the following raw materials in parts by weight: 5 parts of mulberry extract, 8 parts of raspberry extract, 15 parts of strawberry extract, 6 parts of roselle extract, 15 parts of lycium ruthenicum extract, 10 parts of persimmon tannin cracking product, 35 parts of oligomeric inulin and 1 part of citric acid. Mixing according to weight ratio.

Example 3

The composition with the anti-sugar effect comprises the following raw materials in parts by weight: 10 parts of mulberry extract, 5 parts of raspberry extract, 18 parts of strawberry extract, 8 parts of roselle extract, 18 parts of lycium ruthenicum extract, 6 parts of persimmon tannin cracking product, 40 parts of oligoinulin and 3 parts of citric acid. Mixing according to weight ratio.

Example 4

The composition with the anti-sugar effect comprises the following raw materials in parts by weight: 8 parts of mulberry extract, 6 parts of raspberry extract, 19 parts of strawberry extract, 5 parts of roselle extract, 16 parts of lycium ruthenicum extract, 7 parts of persimmon tannin cracking product, 45 parts of oligoinulin and 4 parts of citric acid. Mixing according to weight ratio.

Example 5

The composition with the anti-sugar effect comprises the following raw materials in parts by weight: 9 parts of mulberry extract, 9 parts of raspberry extract, 20 parts of strawberry extract, 10 parts of roselle extract, 15 parts of lycium ruthenicum extract, 5 parts of persimmon tannin cracking product, 50 parts of oligoinulin and 5 parts of citric acid. Mixing according to weight ratio.

Example 6

The composition with the anti-sugar effect comprises the following raw materials in parts by weight: 10 parts of mulberry extract, 10 parts of raspberry extract, 18 parts of strawberry extract, 8 parts of roselle extract, 20 parts of lycium ruthenicum extract, 7 parts of persimmon tannin cracking product, 48 parts of oligoinulin and 3 parts of citric acid. Mixing according to weight ratio.

Evaluation of anti-glycation Effect

The inhibition of AGEs formation by the compositions of examples 2-6 was evaluated using a glucose- β -Lg mimetic system. According to the fluorescence of AGEs, an F-4600 fluorescence spectrometer is adopted to determine the fluorescence intensity of fluorescent AGEs after a blank control group and a composition test group are added for reaction for 7d under the conditions of an excitation wavelength of 325nm and an emission wavelength of 440nm, and the inhibition rate of the composition to the fluorescent AGEs is calculated, wherein the calculation method comprises the following steps:

wherein, F0-fluorescence intensity of blank control group; f1-fluorescence intensity of test group

The results show that the optimal composition (the composition indicated in example 2) of the present invention has a significant function of inhibiting the production of glycosylation products, and the IC thereof₅₀At 378. mu.g/ml, IC of examples 3, 4, 5, 6₅₀475. mu.g/ml, 492. mu.g/ml, 486. mu.g/ml and 428. mu.g/ml, respectively.

Mouse experiments to evaluate the anti-glycation of the compositions of the invention

SPF rats are divided into three groups and fed with normal feed, high-dose AGEs feed and feed (0.5 percent, w/w) containing a composition (the composition in example 2: 5 parts of mulberry extract, 8 parts of raspberry extract, 15 parts of strawberry extract, 6 parts of roselle extract, 15 parts of Lycium ruthenicum extract, 10 parts of persimmon tannin cleavage product, 35 parts of oligoinulin and 1 part of citric acid) respectively, and the expression of RAGE, p38MAPK and NF-kappa B in the liver of the rats is detected by immunohistochemical staining, and the result is shown in figure 6, and shows that the composition can obviously inhibit the activation of a RAGE-p38 MAPK-NF-kappa B signal channel. The expression of the inflammatory factors TNF-alpha (24%) and IL-6 (50%) in the liver is detected by an ELISA method, and the result shows that the composition provided by the invention can obviously reduce the expression of the inflammatory factors. These results indicate that the compositions of the present invention have a significant in vivo anti-glycation effect.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. The composition with the anti-sugar effect is characterized by comprising the following components in parts by weight: 5-10 parts of mulberry extract, 5-10 parts of raspberry extract, 15-20 parts of strawberry extract, 5-10 parts of roselle extract, 15-20 parts of lycium ruthenicum extract, 5-10 parts of persimmon tannin cracking product, 35-50 parts of oligomeric inulin and 1-5 parts of citric acid.

2. The composition with the anti-sugar effect according to claim 1, wherein the content of Cy-3-glc and Cy-3-rut2 major anthocyanins in the mulberry extract is not less than 47.5% and 20.5%; the content of Cy-3-soph and Cy-3-glc 2 major anthocyanins in the red raspberry extract is not less than 30% and 37.5%; the Pg-3-glc content in the strawberry extract is not less than 60%; the content of 3 kinds of anthocyanin Dp-3-sam, Dp-3-glc and Cy-3-glc in the Hibiscus sabdariffa extract is not less than 8.5%, 14.5% and 10%; the content of petunidin-3-rutinoside in the Lycium ruthenicum Murr extract is not less than 60%.

3. The composition with anti-sugar effect as claimed in claim 1, wherein the persimmon tannin cracking product is persimmon procyanidin oligomer obtained by subjecting persimmon tannin to high pressure catalytic hydrogenolysis, the average degree of polymerization is not more than 3, and the content of A-ECG and EGCG dimer is not less than 10%.

4. The composition having an anti-sugar effect according to claim 1, wherein the polymerization degree of oligoinulin is not more than 10.

5. The composition with the sugar-resistant effect according to claim 1, wherein the mulberry extract, the raspberry extract, the strawberry extract, the roselle extract and the lycium ruthenicum extract are prepared by the following steps: weighing and grinding mulberries, red raspberries, strawberries, roselle and lycium ruthenicum mill respectively, extracting by adopting hydrochloric acid methanol, enriching by AB-8 macroporous resin, enriching by medium-pressure countercurrent chromatography, and eluting to obtain a crude extract rich in anthocyanin.

6. Use of the composition with anti-sugar effect according to any one of claims 1 to 5 in the preparation of a medicament or health food for treating, delaying or alleviating ACEs-related diseases.

7. The use according to claim 6, wherein the AGEs-related diseases are diabetes, atherosclerosis, chronic kidney disease, liver disease or neurodegenerative disease.

8. The use according to claim 6, wherein the medicament further comprises pharmaceutically acceptable excipients and the health food further comprises dietetically acceptable excipients.

9. The use according to claim 8, wherein the medicament is in the form of an oral formulation or an injectable formulation.

10. The use according to claim 9, wherein the oral formulation is a tablet, powder, capsule, granule, pill, powder, paste, or oral liquid.