CN111830764A - Auxiliary device for sterile photographing and application method thereof - Google Patents

Abstract

The invention relates to a plant pathology technology and a photography technology, and particularly discloses an auxiliary device for aseptic photography and an application method thereof. The auxiliary device mainly comprises a stabilizing frame, a camera frame and a dish frame. The auxiliary device needs to be matched with a superclean bench for use, and the application method mainly comprises the following steps: 1) operating an ultra-clean workbench: 2) assembling the auxiliary device and the camera: 3) placing and adjusting the material to be photographed: 4) and (3) photographing: 5) and (5) performing operation after photographing. The invention has the advantages that: 1) the photos are taken under the aseptic condition, so that the biological pollution risk caused by the photos can be effectively reduced, and the continuous tracking photographing record of the growth of pathogenic bacteria is facilitated; 2) the influence of shake of a camera held by a hand directly on the definition of an image can be eliminated, and a high-definition picture can be obtained easily; 3) can provide accurate proportional dimension for later photo processing.

Description

Auxiliary device for sterile photographing and application method thereof

Technical Field

The invention relates to a plant pathology technology and a photography technology, in particular to an auxiliary device for aseptic photography and an application method thereof.

Technical Field

Most of plant pathogenic bacteria can grow on a common culture medium without plant hosts, so that the growth and development rules of the pathogenic bacteria and the relation between the pathogenic bacteria and the pathogenic bacteria can be researched and explored by an artificial culture means, and a basis is provided for preventing and controlling plant diseases. The plate is used for culturing pathogenic bacteria, which is a conventional culture mode in common laboratories, plant pathogenic bacteria have various growth traits and/or growth expressions on a plate culture medium, and the quantity of certain growth traits, such as growth speed, thallus weight, sporulation amount and the like, can be accurately recorded. There are also many properties, such as pigment secretion of germs, colony color, colony texture and thickness, and density/looseness of mycelium, which are difficult to be recorded accurately by quantity and not easy to be recorded clearly by characters, but they can be recorded accurately by taking pictures with a camera. Therefore, the photo record of the plate culture material is often needed in practical work. A large amount of environmental microorganisms usually exist in a common laboratory, and the flat dish cover is usually required to be opened for conventional photographing records, so that the photographing records easily cause biological pollution to the flat dish culture material, and the photographed flat dish material is not suitable for research and observation of re-cultivation and material preservation. Obviously, the conventional photographing record has a technical disadvantage which is not easy to overcome.

Disclosure of Invention

The invention aims to provide an auxiliary device for sterile photographing and an application method thereof.

The invention adopts an auxiliary device to be matched with a super clean bench (hereinafter referred to as a super clean bench) for use, solves the technical problems, and adopts the following technical scheme:

1. an auxiliary device for aseptic photographing mainly comprises a stabilizing frame A, a camera frame B and a flat dish frame C.

The stabilizer A belongs to a component for stabilizing the whole auxiliary device and mainly comprises a stabilizer A1, an upper convex frame A2, a lower convex frame A3 and an inclined strut A4, wherein the upper plate surface of the lower convex frame is a bearing surface A5, and the plate surface of the stabilizer vertically connected with the bearing surface A5 is a stabilizing surface A6, as shown in fig. 1 and 2.

The camera frame B is a member for fixing the camera, and is mainly composed of a carrier board B1, an upper hook B2, a lower hook B3, and a band-closing band B4, as shown in fig. 1 and 3.

The flat dish rack C belongs to a component for stabilizing and adjusting the photographing material to adapt to the work of the camera, and mainly comprises a distance plate C1, a color plate C2, a moving plate C3 and a stabilizing card C4, as shown in fig. 1 and 4.

2. An application method of the auxiliary device. The auxiliary device is matched with a superclean bench for use, and the application method comprises the following steps:

1) operating an ultra-clean bench: and normally cleaning and starting up the clean bench according to a conventional program.

2) Assembling the auxiliary device and the camera:

assembling under the normal operation state of the super clean bench, wherein the assembling operation is as follows:

taking out the auxiliary device from the clean storage container, and placing the auxiliary device on the table top of the super clean bench, wherein the end of an upper convex frame A2 of the stabilizing frame is close to an operator, and the end of a stabilizing body A1 is far away from the operator.

Secondly, the oblique strut A4 is supported below the lower convex frame A3 to lift the end of the lower convex frame A3 of the stabilizing frame, so that the stabilizing frame is in an oblique state.

Thirdly, a distance plate C1 of the plate frame member, a color plate C2 and a moving plate C3 are sequentially closely attached to and placed on a bearing surface A5 of the stabilizing frame, and a stable card C4 is placed on a moving plate C3.

Fourthly, the camera is bandlocked on a carrier board B1 of the camera frame by a band-locking belt B4, an upper hook B2 is hung above an upper convex frame A2 of the stabilizing frame, and a lower hook B3 is hung below a lower convex frame A3.

After the auxiliary device and the camera are assembled, beating and shaking off surface particles of the device, and allowing the superclean bench to continuously run for more than 5 minutes;

3) placing and adjusting the material to be photographed:

taking out the culture material to be photographed and recorded from the incubator, transferring the culture material to an ultra-clean working table, and waiting for photographing.

Secondly, a plate of culture material is put on the V-shaped opening of the stable card C4 of the plate rack together with the cover.

Starting the camera to a shooting state, observing the imaging of the material to be shot on the camera screen, adjusting the shot object to the middle of the screen by adjusting the moving plate C3, and adjusting the size of the shot object on the screen by adjusting the distance plate C1.

4) Photographing device

Firstly, taking down the flat dish cover, and focusing and photographing the photographing material according to the normal operation of the camera.

Secondly, after one plate material is photographed, the plate cover is covered, and the plate is taken out from the fixing card C4; and taking another plate to-be-shot material, putting the plate on a stable card C4, and focusing and taking a picture as in the operation of the step I.

And thirdly, repeating the operation of the step II until the materials to be photographed are completely photographed.

Keeping the auxiliary device and the camera in the same photographing setting, photographing the size scales of the common ruler, and preparing for later-stage photo processing.

5) And (3) operation after photographing:

returning the photographed culture material to the original culture environment for continuous culture.

And secondly, taking the auxiliary device out of the clean bench, returning the auxiliary device to a clean storage container for placing, and then shutting down the clean bench.

The invention has the advantages that:

1) the whole process of the plate culture material photographing is in an aseptic environment, and biological pollution caused by conventional photographing can be effectively reduced, so that continuous tracking photographing record can be carried out on character expressions such as colony formation change, color change and the like in the growth process of the same plate material.

2) The influence of shake of a camera held by a hand directly on the definition of an image can be eliminated, and a high-definition picture can be obtained easily.

3) Can provide accurate proportional dimension for later photo processing.

Drawings

Fig. 1 is a schematic side view of the auxiliary device of the present invention. In the figure, a stabilizer A, a camera frame B, and a plate frame C.

Fig. 2 is a schematic structural view of a stabilizer a of the auxiliary device. In the figure, a stabilizer A1, an upper convex frame A2, a lower convex frame A3, an oblique strut A4, a bearing surface A5 and a stabilizing surface A6. The 2-1 diagram illustrates a side structure, and the 2-2 diagram illustrates a front structure.

Fig. 3 is a schematic structural view of a camera frame B of the auxiliary device. In the figure, a carrier plate B1, an upper hook B2, a lower hook B3, and a band-closing band B4. The figure 3-1 illustrates the front structure and the figure 3-2 illustrates the side structure.

Fig. 4 is a schematic structural view of the flat dish holder C of the auxiliary device after being assembled to the stabilizing frame a. In the figure, a distance plate C1, a color plate C2, a moving plate C3 and a stable card C4. The upper convex frame A2, the lower convex frame A3, the bearing surface A5 and the stabilizing surface A6 of the stabilizer A. The figure 4-1 illustrates the side structure and the figure 4-2 illustrates the front structure.

Fig. 5 is a schematic view showing the structure of the operation state of the auxiliary device after assembling the camera and the plate material. In the figure, a stabilizer A1, an upper convex frame A2, a lower convex frame A3, a diagonal support A4, a bearing surface A5 and a stabilizing surface A6 of the stabilizer; a carrier plate B1, an upper hook B2, a lower hook B3 and a band-closing band B4 of the camera frame; a distance plate C1 of the flat dish rack, a color plate C2, a moving plate C3 and a stable card C4. The figure 5-1 illustrates the side structure and the figure 5-2 illustrates the front structure.

FIG. 6 is a graph showing the following of the growth change of the banana vascular wilt bacterium strain Fo-4 on sucrose medium (SA) and potato sucrose medium (PSA) using the auxiliary device of the present invention. In the figure, the cultures are arranged vertically on the same plate; the growth expressions of the same culture days are arranged horizontally, and 0 day means that the plate culture medium is transferred into a strain, then is photographed and transferred to a constant temperature incubator for culture. The black short bars marked below the dishes in the figure are 10mm long.

FIG. 7 is a graph showing the following of the growth change of Uv-111 strain of Uj-smut in potato medium (PA) and potato sucrose medium (PSA) using the auxiliary device of the present invention. In the figure, the cultures are arranged vertically on the same plate; the growth performance of the same culture days is horizontally arranged, 0 day means that the plate culture medium is transferred into a strain, then is photographed and transferred to a constant temperature incubator for culture, and the length of a white short bar marked on the lower part of each plate in the figure is 2 mm.

Detailed Description

The invention is further described below with reference to the figures and examples.

The structure of the auxiliary device

The side structure of an auxiliary device for aseptic photographing is shown in figure 1, and the device mainly comprises a stabilizing frame A, a camera frame B and a plate frame C.

The stabilizer A belongs to a stabilizing part of the whole auxiliary device, and is structurally shown in fig. 2, and mainly comprises a stabilizer A1, an upper convex frame A2, a lower convex frame A3 and a diagonal strut A4, wherein the stabilizer A1 is a rectangular body fastened by plates and is fastened with the upper convex frame A2 and the lower convex frame A3 into a whole. The upper plate surface of the lower convex frame A3 is a bearing surface A5 which plays a role of bearing the components of the flat dish rack C. The surface of the stabilizing body which is vertically connected with the bearing surface A5 is a stabilizing surface A6 which plays a role of stabilizing the components of the flat dish rack C. The cross brace a4 is not securely attached to the stabilizer and functions to brace the lower ledge A3 end of the stabilizer so that the stabilizer is in a tilted working position and changing the height of the cross brace a4 changes the degree of tilt of the stabilizer.

The camera frame B belongs to a part for fixing a camera, and the structure thereof is as shown in fig. 3, and mainly comprises a carrier board B1, an upper hook B2, a lower hook B3 and a band-closing band B4, and the four components are fastened and connected into a whole. Under the action of inclined gravity, the camera frame B can be stably hung on the stabilizing frame A. The upper hook B2 and the lower hook B3 can also slide left and right along the horizontal direction of the upper convex frame a2 and the lower convex frame A3, so that the camera can also move left and right. The camera fixing belts B4 are 2, which are respectively arranged at two sides of the carrier board B1, so that various common cameras or mobile phone cameras can be conveniently fixed on the carrier board B1. The camera frame B is not fixedly connected with the stabilizing frame A, and can be conveniently and freely detached and assembled from the stabilizing frame A.

The flat dish rack C belongs to a part for stabilizing and adjusting the photographing material to adapt to the work of the camera, and the structure of the flat dish rack C is shown in figure 4. The camera stabilizing frame mainly comprises a distance plate C1, a color plate C2, a moving plate C3 and a stabilizing card C4, wherein the distance plate C1 is a component for adjusting the distance between a photographing material and a camera, and mainly comprises a plurality of square plates, the size of each square plate is slightly smaller than the size of a stabilizing frame stabilizing surface A6, the square plates can be conveniently drawn out of or added to the bearing surface A5, and the distance between the photographing material and the camera can be farther or closer by reducing or increasing the number of the square plates. The color plate C2 is a flat plate for adjusting the color of the photographing background, has a size similar to that of the distance plate C1, can be conveniently assembled and disassembled from the bearing surface A5, can be white, black and other colors, and can obtain clearer pictures when a black background is used for common photographing. The moving plate C3 is mainly composed of a plurality of small thin plates, and the addition/subtraction of the small thin plates allows the photographic material to move up/down, while the left/right pushing and pulling of the small thin plates allows the photographic material to move left/right. The fixing clip C4 is a V-shaped opening clip, which can fix the material clips in the shape of round objects on the V-shaped opening, and the fixing clip C4 can also move horizontally on the surface of the moving plate C3. The color of the moving panel C3 and the robust card C4 is the same as the color panel C2. In the photographing working state, due to the inclined gravity, each component of the plate rack C can stably lean against the stabilizing surface A6 of the stabilizing rack. All the components of the flat dish rack are not fixedly connected with the stabilizing frame, so that the flat dish rack can be conveniently assembled, disassembled and assembled.

The auxiliary device for the working state after the camera is installed and the plate material is placed is shown in fig. 5.

In the non-working use state, the components of the auxiliary device which are not fixedly connected can be detached and stored in a clean container (such as a plastic bag or a paper box).

Assembling and application of auxiliary device

The auxiliary device of the invention needs to be matched with an ultra-clean bench for use. Before use, the clean bench is normally cleaned and opened according to the conventional procedures. Then, the auxiliary device is assembled and used on the normally-operated super clean bench, and the specific method for assembling and using is as follows.

And taking out each component of the auxiliary device from the storage container, putting the component on the table top of the super clean bench by lifting the glass baffle of the super clean bench, and then carrying out assembly operation through two operation holes on the glass baffle. The ends of the upper convex frame A2 and the lower convex frame A3 of the stabilizing frame are close to an operator, the end of the stabilizing body A1 is far away from the operator, the oblique strut A4 is arranged below the lower convex frame A3 in a padded mode, the lower convex frame A3 is supported, and the stabilizing frame is in an oblique state, as shown in fig. 1 and 2. The inclination is preferably chosen to suit the observation operation of the operator and to keep the plate material placed behind in a stable state, and the practice shows that the angle between the bearing surface a5 of the stabilizer and the ultra-clean table is preferably about 30 degrees.

And then assembling the flat dish rack. The distance plate C1, the color plate C2 and the moving plate C3 are sequentially closely placed on the bearing surface a5 of the stabilizing frame, and the stabilizing card C4 is placed above the moving plate C3, as shown in fig. 1 and 4.

And then to the assembly camera. Taking out the camera frame, band-locking the camera on the carrier board B1 by a band-locking band B4, then hanging the upper hook B2 above the upper convex frame A2 of the stabilizing frame and hanging the lower hook B3 below the lower convex frame A3, as shown in FIG. 1 and FIG. 3.

After the auxiliary device is assembled, gently patting the stabilizing frame with a hard object (such as scissors or large tweezers commonly used in laboratories) for multiple times, shaking off particles and sundry bacteria with weak adhesion on the surface of the auxiliary device and taking away the particles and sundry bacteria by normally flowing sterile air, and then operating the super clean bench for 5-10 minutes to ensure that the auxiliary device is in a fully sterile environment, and the subsequent photographing operation can be implemented in the sterile environment, so that the risk of biological pollution of a photographing material can be reduced to the lowest degree.

Then, the setting and adjustment of the photographic material can be carried out.

Taking out the culture material needing photographing record from the incubator or the culture room, transferring the culture material onto an ultra-clean table, and waiting for photographing. A flat dish material is put on the V-shaped opening of the flat dish frame stable card C4 together with the dish cover. The plate material was able to rest stably against the panel C2 surface under the influence of the tilting gravity. Starting a camera to a shooting state, observing the imaging of the material to be shot on a camera screen, adjusting the imaging of the material to be shot to the middle of the camera screen by adjusting a moving plate C3, and adjusting the imaging size of the material to be shot on the camera screen to a proper state by adjusting a distance plate C1, wherein the phenomenon that the image is too small and too large is mainly avoided, and when the image is too small, the detailed records of some biological forms are unclear; too large, some biological forms are out of the range of the photograph recording, and imaging near the edges of the screen is prone to distortion. In fact, if the same camera is used for taking a picture, the plate specification is the same, the arrangement conditions of the distance plate C1 and the moving plate C3 can be remembered, and the plate rack can be directly assembled according to the arrangement conditions for next use without adjustment.

After the culture material is placed and adjusted, the photographing operation can be performed. Slightly taking down the flat dish cover, focusing and photographing the photographed material according to the normal operation of the camera, slightly returning the cover after photographing is finished, and taking out the flat dish material from the rack stabilizing card C4; another plate material was then photographed without adjusting the plate holder C member for the same plate. If the size of the plate is changed or the local expression of the growth and development of the plate culture needs to be magnified and photographed, the distance plate C1 and the moving plate C3 of the plate rack need to be adjusted correspondingly to obtain the proper imaging.

Keep auxiliary device and camera in the same setting of shooing, shoot the size scale of the clear ordinary ruler of a scale, can provide scale mark foundation for later stage photo processing, can improve the quality of photo.

Normally, a normal illuminating lamp is arranged in the super-clean bench, the light brightness is suitable for photographing, and if the light brightness is insufficient, the illuminating lamp can be arranged outside the super-clean bench before photographing.

After all photographing operations are finished, the culture material can be returned to the original culture condition to continue culturing and observing records, meanwhile, the auxiliary device is taken out and put back into a clean container to be stored, and then the super clean bench is shut down.

Example 1

The auxiliary device for sterile photographing and the application method thereof are applied, a mobile phone camera VIVO Y66 is installed and is applied to tracking and recording the growth changes of the banana vascular wilt germ strain Fo-4 on two culture media, and after 0, 2, 5, 8 and 11 days of culture, a plate cover is opened to carry out photographing and recording, and the results are as follows:

1) the images of the growth of the germs after 0, 2, 5, and 11 days of culture are shown in FIG. 6;

2) 5 times of uncapping photographing records are carried out on the same plate culture material, and no biological pollution phenomenon appears after 15 days of culture.

Example 2

The auxiliary device for sterile photographing and the application method thereof are applied, a mobile phone camera VIVO Y66 is installed and is applied to tracking and recording the growth change of Uv-111 of ustilaginoidea virens strain on two culture media, and after 0, 2, 5, 8 and 11 days of culture, a plate cover is opened to carry out photographing and recording, and the results are as follows:

1) the images of the growth of the germs after 0, 5, 8, and 11 days of culture are shown in FIG. 7;

2) 5 times of uncapping photographing records are carried out on the same plate culture material, and no biological pollution phenomenon appears after 15 days of culture.

Claims (2)

1. An auxiliary device for aseptic photographing is characterized in that the auxiliary device mainly comprises a stabilizing frame (A), a camera frame (B) and a dish frame (C);

the stabilizing frame (A) belongs to a component for stabilizing the whole auxiliary device and mainly comprises a stabilizing body (A1), an upper convex frame (A2), a lower convex frame (A3) and an inclined strut (A4), wherein the upper plate surface of the lower convex frame is a bearing surface (A5), and the plate surface of the stabilizing body vertically connected with the bearing surface (A5) is a stabilizing surface (A6);

the camera frame (B) belongs to a component for fixing the camera and mainly comprises a carrier plate (B1), an upper hook (B2), a lower hook (B3) and a band-closing band (B4);

the flat dish rack (C) belongs to a component for stabilizing photographing materials and adjusting the photographing materials to be matched with the camera to work, and mainly comprises a distance plate (C1), a color plate (C2), a moving plate (C3) and a stabilizing card (C4).

2. The method of claim 1, wherein the auxiliary device is used in conjunction with an ultra clean bench, and the method comprises the steps of:

1) operating an ultra-clean workbench: normally cleaning and starting up the super clean workbench according to a conventional program;

2) assembling the auxiliary device and the camera:

assembling under the normal operation state of the superclean bench, wherein the assembling operation is as follows:

taking out the auxiliary device from a clean storage container, and putting the auxiliary device on the table top of a super-clean workbench, wherein the end of an upper convex frame (A2) of a stabilizing frame is close to an operator, and the end of a stabilizing body (A1) is far away from the operator;

secondly, supporting an inclined strut (A4) of the stabilizing frame below the lower convex frame (A3) in a cushioning manner, and lifting the end of the lower convex frame (A3) to enable the stabilizing frame to be in an inclined state;

thirdly, a distance plate (C1), a color plate (C2) and a moving plate (C3) of the plate frame member are sequentially and closely arranged on a bearing surface (A5) of the stabilizing frame, and the stabilizing card (C4) is arranged on the surface of the moving plate (C3);

fourthly, a camera is bandlocked on a carrier plate (B1) of the camera frame by a band-locking band (B4), an upper hook (B2) is lapped and hung above an upper convex frame (A2) of the stabilizing frame, and a lower hook (B3) is hung and placed below a lower convex frame (A3);

after the auxiliary device and the camera are assembled, beating and shaking off surface particles of the device, and allowing the superclean bench to continuously run for more than 5 minutes;

3) placing and adjusting the material to be photographed:

taking out the culture material to be photographed and recorded from the incubator, transferring the culture material to an ultra-clean working table, and waiting for photographing;

secondly, a plate culture material is put on a V-shaped opening of a stable card (C4) of the plate rack together with a cover;

starting the camera to a shooting state, observing the imaging of the material to be shot on a camera screen, adjusting the shot object to the middle of the screen by adjusting the moving plate (C3), and adjusting the size of the shot object on the screen by adjusting the distance plate (C1);

4) and (3) photographing:

taking down a flat dish cover, and focusing and photographing materials according to the normal operation of a camera;

secondly, after one plate material is photographed, the plate cover is covered, and the plate is taken out from the stabilizing card (C4); taking another plate to-be-shot material, putting the plate on a stabilizing card (C4), and focusing and taking pictures as in the step I;

repeating the operation step II until all the materials to be photographed are photographed;

keeping the auxiliary device and the camera in the same photographing setting, photographing the size scales of the common ruler, and preparing for later-stage picture processing;

5) and (3) operation after photographing:

returning the photographed culture material to the original culture environment for continuous culture;

and secondly, taking the auxiliary device out of the clean bench, returning the auxiliary device to a clean storage container for placing, and then shutting down the clean bench.

Images