CN111829826A - Blood sample extraction device for gene marker detection - Google Patents

Blood sample extraction device for gene marker detection Download PDF

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Publication number
CN111829826A
CN111829826A CN202010592468.XA CN202010592468A CN111829826A CN 111829826 A CN111829826 A CN 111829826A CN 202010592468 A CN202010592468 A CN 202010592468A CN 111829826 A CN111829826 A CN 111829826A
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China
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chamber
gear
blood sample
piston
driving mechanism
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CN202010592468.XA
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Chinese (zh)
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周猛
孙杰
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Wenzhou Medical University
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Wenzhou Medical University
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Priority to CN202010592468.XA priority Critical patent/CN111829826A/en
Publication of CN111829826A publication Critical patent/CN111829826A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices

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  • Life Sciences & Earth Sciences (AREA)
  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a blood sample extraction device for detecting a gene marker, which is characterized in that a liquid suction head is arranged in a blood sample, a first driving mechanism drives a first piston to move in a second chamber to suck air in the first chamber into the second chamber, so that the blood sample enters the first chamber from the liquid suction head under the action of air pressure difference, the blood sample is quickly and automatically extracted, and the quantitative extraction of the blood sample can be realized by accurately controlling the first driving mechanism; and the first filtering membrane is arranged at one end of the first chamber close to the liquid suction head, so that the blood sample is filtered, and serum which is easier to store and has more medical value in the blood sample is extracted.

Description

Blood sample extraction device for gene marker detection
Technical Field
The invention relates to the technical field of medical equipment, in particular to a blood sample extraction device for detecting gene markers.
Background
In modern medicine, the testing of blood is one of the most important techniques for clinical diagnosis. Before the human gene markers are detected, a blood sample needs to be extracted, and the blood sample in the medical apparatus is usually extracted by using a needle tube and a test tube, and then the blood sample of the human body is put into the test tube to be detected by a professional. However, most of the existing extraction methods are manual operations, and the problem of excessive or small amount of blood extraction is easily caused when the test tube is used for extraction, so that the detection result is not accurate enough; moreover, due to the self-coagulation mechanism of blood, the long-term preservation of blood is not favorable for the accuracy of detection, and therefore, the efficiency of blood sample extraction needs to be improved.
Disclosure of Invention
In view of the above, embodiments of the present invention are directed to provide a blood sample extraction apparatus for detecting a gene marker, in which a liquid suction head is placed in a blood sample, a first driving mechanism drives a first piston to move in a second chamber to draw air in the first chamber into the second chamber, so that the blood sample enters the first chamber from the liquid suction head under the action of an air pressure difference, the blood sample is rapidly and automatically extracted, and quantitative extraction of the blood sample can be realized by accurately controlling the first driving mechanism; and the first filtering membrane is arranged at one end of the first chamber close to the liquid suction head, so that the blood sample is filtered, and serum which is easier to store and has more medical value in the blood sample is extracted.
An embodiment of the present invention provides a blood sample extraction device for detecting a gene marker, including: a liquid suction head; a first chamber in communication with the pipette head; a second chamber in movable communication with the first chamber; the first piston is arranged in the second chamber and is in sealing connection with the second chamber; the first driving mechanism is connected with the first piston and is used for driving the first piston to reciprocate; wherein, the first chamber is provided with a first filtering membrane close to one end of the liquid suction head.
In one embodiment, the first chamber is in communication with the third chamber at a location proximate to the first filter membrane and distal to the pipetting head.
In one embodiment, the third chamber is provided with a second filter membrane near one end of the first chamber.
In one embodiment, the pore size of the second filter membrane is smaller than the pore size of the first filter membrane.
In one embodiment, the third chamber is in movable communication with a fourth chamber, and a second piston sealingly connected to the fourth chamber is disposed in the fourth chamber.
In one embodiment, the second piston is connected to a second drive mechanism that drives the second piston in a reciprocating motion.
In an embodiment, the first driving mechanism is in transmission connection with a first gear, the first piston is in transmission connection with a second gear, a first transmission rack is arranged between the first gear and the second gear, and the first gear and the second gear are in transmission connection with the first transmission rack respectively.
In an embodiment, the second piston is in transmission connection with a third gear, a second transmission rack is arranged between the first gear and the third gear, and the first gear and the third gear are in transmission connection with the second transmission rack respectively.
In one embodiment, the first drive rack includes a first groove portion engaged with the second gear and a first flat portion not engaged with the second gear, and the second drive rack includes a second groove portion engaged with the third gear and a second flat portion not engaged with the third gear, the first groove portion corresponding to the second flat portion, and the first flat portion corresponding to the second groove portion.
In an embodiment, a speed reducer is disposed between the first driving mechanism and the first and second transmission racks.
According to the blood sample extraction device for detecting the gene marker, which is provided by the embodiment of the invention, the liquid suction head is arranged in the blood sample, the first piston is driven by the first driving mechanism to move in the second chamber, so that air in the first chamber is pumped into the second chamber, the blood sample enters the first chamber from the liquid suction head under the action of air pressure difference, the blood sample is quickly and automatically extracted, and the quantitative extraction of the blood sample can be realized by accurately controlling the first driving mechanism; and the first filtering membrane is arranged at one end of the first chamber close to the liquid suction head, so that the blood sample is filtered, and serum which is easier to store and has more medical value in the blood sample is extracted.
Drawings
Fig. 1 is a schematic structural diagram of a blood sample extraction device for detecting a gene marker according to an embodiment of the present application.
Fig. 2 is a schematic structural diagram of a blood sample extraction device for detecting gene markers according to another embodiment of the present disclosure.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Further, in the exemplary embodiments, since the same reference numerals denote the same components having the same structure or the same steps of the same method, if an embodiment is exemplarily described, only a structure or a method different from the already described embodiment is described in other exemplary embodiments.
Throughout the specification and claims, when one element is described as being "connected" to another element, the one element may be "directly connected" to the other element or "electrically connected" to the other element through a third element. Furthermore, unless explicitly described to the contrary, the term "comprising" and its corresponding terms should only be taken as including the stated features, but should not be taken as excluding any other features.
Fig. 1 is a schematic structural diagram of a blood sample extraction device for detecting a gene marker according to an embodiment of the present application. As shown in fig. 1, the extraction device includes: the liquid suction head comprises a liquid suction head 1, a first chamber 2, a second chamber 3, a first piston 4 and a first driving mechanism 5. Wherein, imbibition head 1 and first cavity 2 intercommunication, first cavity 2 and the activity of second cavity 3 intercommunication, first piston 4 sets up in second cavity 3 and with second cavity 3 sealing connection, first actuating mechanism 5 is connected with first piston 4 for drive first piston 4's reciprocating motion, first cavity 2 is close to imbibition head 1 one end and sets up first filtration diaphragm 6. When a blood sample needs to be taken, the pipette tip 1 is placed in the blood sample (which may be a vessel for containing the blood sample, or the pipette tip 1 may be directly inserted into a human vein), the first driving mechanism 5 drives the first piston 4 to move towards one end far away from the first chamber 2, so as to draw air in the first chamber 2 into the second chamber 3, thereby generating an air pressure difference between the inside and the outside of the first chamber 2, and the blood sample is pressed into the first chamber 2 by the air pressure difference, thereby realizing the automatic extraction of the blood sample, simultaneously arranging the first filtering diaphragm 6 at the position of the first chamber 2 close to one end of the liquid suction head 1, removing the thrombocytes, the leucocytes, the erythrocytes, the fibrinogen and the like in the blood sample by utilizing the first filtering diaphragm 6, so as to obtain the serum sample, thereby prolonging the preservation time of the blood sample and improving the detection precision of the blood sample. Moreover, first cavity 2 and 3 swing joint of second cavity, after having drawed the blood sample, can be through the first cavity 2 of simple dismantlement in order to realize the change of first cavity 2, can guarantee not to interfere with each other between the different blood samples, also can improve the convenience of changing first cavity 2.
In an alternative embodiment of the present application, the extraction of a quantitative blood sample can be achieved by precisely controlling the first drive mechanism 5. In a further alternative embodiment, a controller may be provided to flexibly select the volume of blood sample taken via the controller or control buttons on the controller. For example, commonly used volume buttons (e.g., 10ml, 20ml, etc.) are provided on the controller, and medical staff manually selects the corresponding button according to actual needs to obtain a blood sample of the corresponding volume. In an alternative embodiment of the present application, the material of the pipette tip 1 may be metal, glass, plastic, resin, ceramic, or the like. It should be understood that, in the embodiment of the present application, different materials of the liquid suction head 1 may be selected according to requirements of an actual application scenario, and the embodiment of the present application does not limit this. In an alternative embodiment of the present application, a sealing gasket may be disposed between the first piston 4 and the second chamber 3, and the sealing performance of the first piston 4 and the second chamber 3 may be ensured by the sealing gasket, so as to ensure an accurate corresponding relationship between the movement displacement of the first piston 4 and the blood sample extraction volume. In an alternative embodiment of the present application, the material of the first chamber 2 may be glass, plastic, resin, or ceramic, which is not limited in the embodiment of the present application. In an optional embodiment of the present application, the material of the first filtering membrane 6 may be ceramic, metal, polyethylene, graphene, or carbon nanotube, and the specific material of the first filtering membrane 6 is not limited in the present application. In an optional embodiment of the present application, the outer surface of the first chamber 2 may be provided with a scale, and by setting the scale, the volume of the blood sample in the first chamber 2 may be known in real time, so as to achieve manual intervention on the volume of the extracted blood sample.
According to the blood sample extraction device for detecting the gene marker, which is provided by the embodiment of the invention, the liquid suction head is arranged in the blood sample, the first piston is driven by the first driving mechanism to move in the second chamber, so that air in the first chamber is pumped into the second chamber, the blood sample enters the first chamber from the liquid suction head under the action of air pressure difference, the blood sample is quickly and automatically extracted, and the quantitative extraction of the blood sample can be realized by accurately controlling the first driving mechanism; and the first filtering membrane is arranged at one end of the first chamber close to the liquid suction head, so that the blood sample is filtered, and serum which is easier to store and has more medical value in the blood sample is extracted.
In an alternative embodiment of the present application, as shown in fig. 1, the first chamber 2 communicates with the third chamber 7 at a position close to the first filter membrane 6 and far from the pipette head 1. One side of keeping away from the pipette head 1 at first filter membrane 6 sets up third chamber 7, and blood sample can get into third chamber 7 after 6 filters through first filter membrane promptly, utilizes third chamber 7 can draw the serum sample in the blood sample to avoid directly obtaining the serum sample and sneaking into blood platelet etc. in by first chamber 2, thereby improve the purity of serum sample.
In an alternative embodiment of the present application, as shown in fig. 1, the third chamber 7 is provided with a second filter membrane 8 near one end of the first chamber 2. Through setting up second filter membrane 8, can further filter the blood sample after 6 filters of first filter membrane to further improve serum sample's purity. In an alternative embodiment of the present application, the pore size of the second filter membrane 8 is smaller than the pore size of the first filter membrane 6. In order to avoid the filtering speed of first filter membrane 6 to hang down and cause the effect that blood sample drawed to hang down excessively, can be with the great of the aperture setting of first filter membrane 6, utilize the great first filter membrane 6 in aperture to carry out the first filtration to blood sample, then utilize the less second filter membrane 8 in aperture to carry out the second filtration to blood sample, improve the filter effect under the prerequisite that does not influence the effect of drawing. In a further alternative embodiment of the present application, the pore size range of the first filter membrane 6 may be 100-500 nm, and preferably, the pore size range of the first filter membrane 6 may be 200-400 nm. The pore size of the first filter membrane 6 can be flexibly selected according to the size of other substances such as platelets in the blood sample, which is not limited in the embodiment of the present application.
In an alternative embodiment of the present application, as shown in fig. 1, the third chamber 7 is movably connected to the fourth chamber 9, and a second piston 10 is disposed in the fourth chamber 9 and hermetically connected to the fourth chamber 9. Through setting up fourth chamber 9 and second piston 10, can utilize the motion of second piston 10 to draw the air in third chamber 7 into in fourth chamber 9, make inside and outside a third chamber 7 produce a pressure differential, this pressure differential can extrude the blood sample in the first chamber 2 with third chamber 7 intercommunication to the third chamber 7 in, thereby realize the transfer of blood sample, and thoroughly separate serum and other compositions in the blood sample, can be simple through the structure of this application embodiment realize the separation of serum and other compositions in the blood sample, and can not arouse the surge of blood sample at the in-process of transferring, thereby avoid other compositions to get into in the serum because of the effect of surge power, the purity of serum has been improved promptly. In addition, the third chamber 7 is in movable communication with the fourth chamber 9, for example via a removable hose, which allows for easy removal and replacement of the third chamber 7 after transfer of serum to the third chamber 7. In an alternative embodiment of the present application, the third chamber 7 may be disposed outside the first chamber 2, or may be disposed directly inside the first chamber 2 to save space, and the specific positional relationship between the first chamber 2 and the third chamber 7 is not limited in the embodiment of the present application.
In an alternative embodiment of the present application, as shown in fig. 1, the second piston 10 is connected to a second driving mechanism 11, and the second driving mechanism 11 drives the second piston 10 to reciprocate. By providing the second driving mechanism 11, the second piston 10 can be automatically moved back and forth, thereby automatically transferring the serum sample. It should be understood that the second driving mechanism 11 in the embodiment of the present application may also be controlled by the controller in the above-described embodiment, or by a separate controller, and the embodiment of the present application is not limited to the control unit and the control method of the second driving mechanism 11.
In an alternative embodiment of the present application, as shown in fig. 1, the first driving mechanism 5 is in transmission connection with a first gear 12, the first piston 4 is in transmission connection with a second gear 13, a first transmission rack 14 is disposed between the first gear 12 and the second gear 13, and the first gear 12 and the second gear 13 are in transmission connection with the first transmission rack 14 respectively. The first gear 12 and the second gear 13 can be in transmission connection with each other by a first transmission rack 14 which is in transmission connection (e.g. meshed) with the first gear 12 and the second gear 13, and the first driving mechanism 5 drives the first gear 12 to rotate, so as to drive the second gear 13 to rotate, and further, the first piston 4 can reciprocate. A similar transmission between the second drive mechanism 11 and the second piston 10 is also possible, and will not be described in detail here. It should be understood that other transmission manners may be selected in the embodiments of the present application to realize that the first driving mechanism 5 drives the first piston 4 and the second driving mechanism 11 drives the second piston 10, which is not limited in the embodiments of the present application.
Fig. 2 is a schematic structural diagram of a blood sample extraction device for detecting gene markers according to another embodiment of the present disclosure. As shown in fig. 2, the second piston 10 is in transmission connection with a third gear 15, a second transmission rack 16 is arranged between the first gear 12 and the third gear 15, and the first gear 12 and the third gear 15 are in transmission connection with the second transmission rack 16 respectively. In the embodiment of the present application, only one driving mechanism may be provided, for example, only the first driving mechanism 5 is provided to drive the first piston 4 and the second piston 10 to reciprocate simultaneously, such as a transmission structure of the first piston 4, or a third gear 15 may be provided to be connected to the second piston 10, and a second transmission rack 16 may be provided between the first gear 12 and the third gear 15 to realize the transmission connection of the first driving mechanism 5 and the second piston 10. In an alternative embodiment of the present application, a speed reducer may be disposed between the first driving mechanism 5 and the first and second transmission racks 14 and 16. Through setting up the speed reducer, the round trip motion of first piston 4 and second piston 10 can be more accurate control to the blood sample is drawed to the accuracy, and can set up the speed reducer that has the variable speed function or set up the derailleur alone, thereby realize the transmission connection between first actuating mechanism 5 and first driving rack 14 or the second driving rack 16, realize that first actuating mechanism 5 alone is connected with first driving rack 14 transmission through the derailleur promptly, or first actuating mechanism 5 alone is connected with second driving rack 16 transmission.
In an alternative embodiment of the present application, as shown in fig. 2, the first driving rack 14 includes a first groove portion 141 engaged with the second gear 12 and a first flat portion 142 not engaged with the second gear 12, the second driving rack 16 includes a second groove portion 161 engaged with the third gear 15 and a second flat portion 162 not engaged with the third gear 15, the first groove portion 141 corresponds to the second flat portion 162, and the first flat portion 142 corresponds to the second groove portion 161. By providing the first groove portion 141 to be engaged with the second gear 12 and the first flat portion 142 to be disengaged from the second gear 12, it is possible to realize that the first gear 12 drives the first piston 4 through the first groove portion 141 and does not drive the first piston 4 through the first flat portion 142. Similarly, the first gear 12 can drive the second piston 10 through the second groove portion 161 and not drive the second piston 10 through the second flat portion 162, and the first groove portion 141 corresponds to the second flat portion 162 and the first flat portion 142 corresponds to the second groove portion 161, that is, the first gear 12 (or the first driving mechanism 5) does not drive the second piston 10 when driving the first piston 4, and the first gear 12 (or the first driving mechanism 5) does not drive the first piston 4 when driving the second piston 10, thereby achieving the function of alternate driving.
In an alternative embodiment of the present application, the first driving mechanism 5 and the second driving mechanism 11 in the above embodiments may be devices having a driving function, such as motors. In an optional embodiment of the present application, the extraction device in this embodiment of the present application may further include a driving power source, where the driving power source is electrically connected to the first driving mechanism 5 and/or the second driving mechanism 11, and is used to increase the required electric energy for the first driving mechanism 5 and/or the second driving mechanism 11, and in a further optional embodiment, the driving power source may be an energy storage device such as a lithium battery, which has an electric energy storage function.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and the like that are within the spirit and principle of the present invention are included in the present invention.

Claims (10)

1. A blood sample extraction device for detecting a gene marker, comprising:
a liquid suction head;
a first chamber in communication with the pipette head;
a second chamber in movable communication with the first chamber;
the first piston is arranged in the second chamber and is in sealing connection with the second chamber; and
the first driving mechanism is connected with the first piston and is used for driving the first piston to reciprocate;
wherein, the first chamber is provided with a first filtering membrane close to one end of the liquid suction head.
2. The device of claim 1, wherein the first chamber communicates with a third chamber proximate the first filter membrane and distal to the pipetting head.
3. The device of claim 2, wherein the third chamber is provided with a second filter membrane adjacent an end of the first chamber.
4. The device of claim 3, wherein the pore size of the second filter membrane is smaller than the pore size of the first filter membrane.
5. The device of claim 2, wherein the third chamber is in fluid communication with a fourth chamber, and a second piston is disposed in the fourth chamber and is sealingly coupled to the fourth chamber.
6. The device of claim 5, wherein the second piston is coupled to a second drive mechanism that drives the second piston to and from motion.
7. The device of claim 5, wherein the first driving mechanism is in transmission connection with a first gear, the first piston is in transmission connection with a second gear, a first transmission rack is arranged between the first gear and the second gear, and the first gear and the second gear are in transmission connection with the first transmission rack respectively.
8. The device of claim 7, wherein the second piston is in transmission connection with a third gear, a second transmission rack is arranged between the first gear and the third gear, and the first gear and the third gear are in transmission connection with the second transmission rack respectively.
9. The apparatus of claim 8, wherein the first drive rack includes a first recessed portion that engages the second gear and a first flat portion that does not engage the second gear, and the second drive rack includes a second recessed portion that engages the third gear and a second flat portion that does not engage the third gear, the first recessed portion corresponding to the second flat portion, and the first flat portion corresponding to the second recessed portion.
10. The device of claim 8, wherein a speed reducer is disposed between the first drive mechanism and the first and second drive racks.
CN202010592468.XA 2020-06-24 2020-06-24 Blood sample extraction device for gene marker detection Pending CN111829826A (en)

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CN202010592468.XA CN111829826A (en) 2020-06-24 2020-06-24 Blood sample extraction device for gene marker detection

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Application Number Priority Date Filing Date Title
CN202010592468.XA CN111829826A (en) 2020-06-24 2020-06-24 Blood sample extraction device for gene marker detection

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205041856U (en) * 2015-10-13 2016-02-24 广州新克力生物科技有限公司 White cell filtration device of blood
CN206616221U (en) * 2017-02-21 2017-11-07 北京旌准医疗科技有限公司 A kind of virus filtration adsorbent equipment
CN207423629U (en) * 2017-11-15 2018-05-29 济南金域医学检验中心有限公司 A kind of blood testing device for filtering impurities
CN108421596A (en) * 2018-02-06 2018-08-21 重庆峰创科技有限公司 A kind of gear driving oscillatory type activated carbon grinding screening plant
CN207991883U (en) * 2018-04-10 2018-10-19 杭州普优生物技术有限公司 A kind of serum separate type pipe
CN108795729A (en) * 2018-09-28 2018-11-13 湖南乐准智芯生物科技有限公司 A kind of biochip and its micro liquid sampling structure
CN110018024A (en) * 2019-05-07 2019-07-16 河南康谱生物科技有限公司 A kind of blood test extraction element

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205041856U (en) * 2015-10-13 2016-02-24 广州新克力生物科技有限公司 White cell filtration device of blood
CN206616221U (en) * 2017-02-21 2017-11-07 北京旌准医疗科技有限公司 A kind of virus filtration adsorbent equipment
CN207423629U (en) * 2017-11-15 2018-05-29 济南金域医学检验中心有限公司 A kind of blood testing device for filtering impurities
CN108421596A (en) * 2018-02-06 2018-08-21 重庆峰创科技有限公司 A kind of gear driving oscillatory type activated carbon grinding screening plant
CN207991883U (en) * 2018-04-10 2018-10-19 杭州普优生物技术有限公司 A kind of serum separate type pipe
CN108795729A (en) * 2018-09-28 2018-11-13 湖南乐准智芯生物科技有限公司 A kind of biochip and its micro liquid sampling structure
CN110018024A (en) * 2019-05-07 2019-07-16 河南康谱生物科技有限公司 A kind of blood test extraction element

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Application publication date: 20201027