CN111821253A

CN111821253A - Chlorophyll spray bacteriostatic liquid formula for diabetic gangrene and extraction process thereof

Info

Publication number: CN111821253A
Application number: CN202010798665.7A
Authority: CN
Inventors: 卢筱桂
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-08-11
Filing date: 2020-08-11
Publication date: 2020-10-27

Abstract

The invention belongs to the technical field of chlorophyll extraction, and particularly relates to a chlorophyll spray bacteriostatic solution formula for diabetic gangrene, which comprises aloe, folium artemisiae argyi, poplar leaf, pagodatree leaf, fig leaf and corn. The invention also relates to an extraction process of the formula, which comprises the following steps: the method comprises the following steps: after aloe, folium artemisiae argyi, poplar leaves, sophora japonica leaves, fig leaves and corn are crushed, leaching with an organic solvent, stirring, and keeping the constant temperature of about 70 ℃ for 3-4 hours; step two: after leaching, filtering and separating to obtain a dark green solution; step three: concentrating the filtered leaching solution, adding NaOH into the concentrated solution, stirring at about 70 ℃ for about 1h, cooling, extracting with petroleum ether, and separating out the petroleum ether; step four: adding CuS0₄Stirring the solution at a constant temperature of about 70 ℃ for a period of time, cooling, standing, and then performing suction filtration to obtain a crude product; step five: washing the precipitate with distilled water, 40% ethanol and petroleum ether, dissolving the precipitate with acetone, and filtering.

Description

Chlorophyll spray bacteriostatic liquid formula for diabetic gangrene and extraction process thereof

Technical Field

The invention belongs to the technical field of chlorophyll extraction, and particularly relates to a chlorophyll spray bacteriostatic solution formula for diabetic gangrene and an extraction process thereof.

Background

Chlorophyll has great potential as a metalloporphyrin compound in clinical treatment, food and daily chemical industry. The central ion is replaced or the external frame is modified, so that a purposeful, promising, efficient and low-toxicity large family of medicines, health-care products and foods can be obtained. With the deep development of chlorophyll chemical technology and chlorophyll product bioactivity research, the application range of the chlorophyll product will be continuously expanded. The chlorophyll has abundant and inexhaustible cheap chlorophyll resources, and researches prove that the chlorophyll has no toxicity, and the derivatives and degradation products of the chlorophyll are low-toxicity substances, so that the advantages are fully utilized, the production of the chlorophyll and the derivatives thereof is developed, the chlorophyll is applied to various fields, scientific research is taken as productivity, and unlimited social and economic benefits can be created. Chlorophyll and derivatives thereof are cyclic tetrapyrrole compounds containing a pentone ring, and the derivatives mainly mean that the core magnesium atom of the chlorophyll is replaced by metal ions such as palladium, zinc, copper, nickel, cobalt, iron and the like to generate derivatives which are stable to light and heat, and simultaneously lose fluorescence. Due to the functional and structural characteristics and physiological activities in various aspects, the compounds are natural organic compounds with multiple purposes and are widely applied to the aspects of medicines, foods, daily chemical industry and the like. With the research on chlorophyll and derivatives thereof, the research finds that the chlorophyll has extremely wide development space in the fields of medicine and health care, food, daily chemical industry and the like, and the deep development of products thereof can create considerable social and economic benefits for society.

Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia, which is caused by defects in insulin secretion or impaired biological action, or both. Blood sugar is an energy supply substance in a human body, and if the blood sugar cannot enter related tissue cells immediately and is retained in blood for a long time, the blood sugar can cause serious damage to the human body. Chronic high levels of blood glucose can cause chronic damage to the eyes, kidneys, heart, blood vessels, nerves, dysfunction. The current diabetes in China is in an ascending trend, and the incidence rate of diabetes in China reaches 11.6% at present, and the diabetes becomes the seventh disease which approximately causes human death.

Diabetes mellitus is not the main cause of disability or death of the human body, and various complications caused by diabetes mellitus are the main causes of disability and death of the patient. Among the many complications of diabetes, impaired wound healing in diabetics is a typical complication of diabetes. After the body of a diabetic has a wound, the diabetic is very easy to cause the phenomena of ulcer, infection, abscess and the like because the diabetic cannot heal for a long time. If a traumatic wound occurs on the foot, it may cause diabetic foot, and even amputation in the severe case. Wound healing is a complex and delicate process, involving a variety of cells and molecules that coordinate with each other to perform wound repair. Normal wound healing consists of a coagulation phase, an inflammation phase, a proliferation phase, and a repair phase, with no distinct boundaries between the phases, which occur continuously and alternate with each other. When trauma occurs, firstly, the organism has hemostatic reaction, platelet aggregation and fibrin hemostatic plug formation, platelets release a large amount of growth factors, and the growth factors promote vasoconstriction and increase vascular permeability; on the other hand, attract inflammatory cells to migrate to the wound site. This process occurs hours after the wound has emerged, and within the following 2 to 3 days, the wound heals into the inflammatory phase, and various inflammatory cells, such as neutrophils and macrophages, enter the wound, they can phagocytose invading bacteria and digest necrotic tissue cell debris, playing a role in cleaning the wound surface, and they also synthesize and release various growth factors and regulatory molecules. In the proliferation stage, the proliferation speed of various cells is increased, for example, the migration and proliferation speed of keratinocytes can promote re-epithelialization, and the re-epithelialization provides a protective screen with the outside for normal wounds so as to prevent the body from being infected; increased endothelial cell migration and proliferation can promote the formation of new blood vessels, provide a good blood supply for wound healing, and deliver sufficient oxygen and nutrients; while increased fibroblast migration and proliferation may promote the formation of granulation tissue. During the repair phase, extracellular matrix deposition, the rapid formation and deposition of extracellular matrix, provides scaffolding for cells involved in wound healing, while providing matrix support for regenerative repair and vessel expansion during angiogenesis.

Based on the healing process of human body wound, when a normal healthy human body has wound, only the corresponding wound medicine with the functions of sterilization, infection prevention, debridement and disinfection is needed to be pasted or orally taken, the wound medicine basically completes the wound repair by various cells and substances in the human body, but the wound medicine has no obvious amplification effect on the wound repair of the cells and the substances in the human body, so the traditional wound medicine has very limited effect on the wound repair of the diabetic wound. For the reasons, no effective method for treating diabetic wounds and diabetic complications such as ulcer, erosion and diabetic foot exists clinically at present. The main wound treatment means are blood sugar and blood fat reduction, antibiotic treatment and very few medicines for directly promoting wound healing. Therefore, the search and development of a drug capable of promoting the healing of diabetic wounds is an urgent problem to be solved.

In addition, the traditional chlorophyll extraction process has the following defects:

1. the material has active property and unstable structure, is easy to be decomposed in response under strong light, and cannot be stored normally;

2. the absorption is not easy, the utilization rate is low and is only 12.36 to 20.50 percent;

3. can not be widely applied, has low bacteriostasis rate to escherichia coli, staphylococcus aureus and candida albicans, and has small bacteriostasis.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides the chlorophyll spraying bacteriostatic solution formula for diabetic gangrene, which can directly promote the healing of diabetic wounds and has better effect; the invention also provides an extraction process of the formula, obviously enhances the stability of chlorophyll, greatly improves the utilization rate, is convenient for normal state storage, and can be popularized and used in a large range.

In order to solve the technical problems, the invention adopts the following technical scheme:

the chlorophyll gunning bacteriostatic solution formula for diabetic gangrene comprises the following components:

aloe vera: 30 to 80 portions of

Folium artemisiae argyi: 50 to 100 parts of

Poplar leaf: 20 to 50 portions of

And (3) pagodatree leaves: 20 to 50 portions of

Fig leaves: 30 to 80 portions of

Corn: 80-180 parts.

Further, 55 parts of aloe, 75 parts of folium artemisiae argyi, 35 parts of poplar leaves, 35 parts of sophora leaves, 55 parts of fig leaves and 130 parts of corn.

Further, the aloe is 30 parts, the folium artemisiae argyi is 50 parts, the poplar leaves are 20 parts, the pagoda leaves are 20 parts, the fig leaves are 30 parts, and the corn is 80 parts.

Further, the aloe is 80 parts, the folium artemisiae argyi is 100 parts, the poplar leaves are 50 parts, the pagoda leaves are 50 parts, the fig leaves are 80 parts, and the corn is 180 parts.

The extraction process of the chlorophyll woundplast bacteriostatic agent formula for diabetic gangrene comprises the following steps:

the method comprises the following steps: after aloe, folium artemisiae argyi, poplar leaves, sophora japonica leaves, fig leaves and corn are crushed, leaching with an organic solvent, stirring, and keeping the constant temperature of about 70 ℃ for 3-4 hours;

step two: after leaching, filtering and separating to obtain a dark green solution;

step three: concentrating the filtered leaching solution, adding NaOH into the concentrated solution, stirring at about 70 ℃ for about 1h, cooling, extracting with petroleum ether, and separating out the petroleum ether;

step four: adding CuS0₄Stirring the solution at a constant temperature of about 70 ℃ for a period of time, cooling, standing, and then performing suction filtration to obtain a crude product;

step five: washing the precipitate with distilled water, 40% alcohol and petroleum ether, dissolving the precipitate with acetone, filtering, and drying the solid to obtain the final product.

Further, the organic solvent in the first step is one or more of butanol, methyl chloride, petroleum ether, isopropanol, trichloroethylene, ethanol, acetone, dimethylformamide and dimethyl sulfoxide.

Further, the organic solvent in the first step is a 95% ethanol solution.

the method comprises the following steps: weighing aloe, folium artemisiae argyi, poplar leaves, sophora japonica leaves, fig leaves and corn, crushing the aloe, folium artemisiae argyi, poplar leaves, sophora japonica leaves, fig leaves and corn, mixing the crushed aloe, folium artemisiae argyi, poplar leaves, sophora japonica leaves, fig leaves and corn with 8-10 parts of sodium carbonate for later use;

step two: adding 8000-10000 parts of water to obtain a culture medium system, adjusting the pH value of the culture medium system to 6.0-6.5 by using sodium hydroxide, stirring, and sterilizing for 25-35 minutes to obtain a culture medium;

step three: inoculating CGMCC0607 strain into the culture medium, dynamically culturing for 18-26 hours, stirring and filtering, and collecting filtrate.

Further, under the condition of 60-70 ℃, the CGMCC0607 strain is inoculated to the culture medium, and dynamic culture is carried out under the condition of 60-70 ℃.

Further, after the dynamic culture is finished, 0.5 wt% -1.0 wt% of phenol and 0.5 wt% -1.0 wt% of diatomite are added into the culture system, and then stirring and filtering are carried out.

Compared with the prior art, the invention has the following beneficial effects:

1. in the chlorophyll invasive spraying bacteriostat detection, the detection results of coliform group, pseudomonas aeruginosa, staphylococcus aureus, hemolytic streptococcus, total bacterial colony number and total fungal colony number meet the requirements of GB 15979 plus 2002 hygienic Standard for Disposable sanitary products.

2. The chlorophyll sprayable bacteriostasis liquid has bacteriostasis rate on colibacillus (ATCC25922), staphylococcus aureus (ATCC 6538) and candida albicans (ATCC 10231) reaching the standard value (bacteriostasis rate is more than or equal to 90 percent) of GB 15979 and 2002 appendix C4, and has stronger bacteriostasis effect on the tested strains.

3. The pH value (25 ℃) of the chlorophyll woundful bacteria-spraying liquid is 6.58.

4. The chlorophyll sprayable bacteriostatic solution has the arsenic content of less than 0.05mg/kg, the lead content of less than 0.05mg/kg and the mercury content of less than 0.05mg/kg, and meets the standard requirements of technical safety standards of cosmetics (2015 edition).

5. The content of polyhexamethylene biguanide in the chlorophyll sprayable bacteriostatic liquid is 0.0172%.

6. After the chlorophyll sprayable bacteriostatic solution is stored for 14 days at 54 ℃, the content of polyhexamethylene biguanide is 0.0168 percent, the reduction rate is 2.3 percent compared with that before the storage, the requirement that the reduction rate of effective components in disinfection technical specification (2002 edition) -2.2.3.2.1 is not more than 10 percent is met, and the storage period of validity of a sample can be determined to be 1 year.

7. The formula of the invention can directly promote the healing of the diabetic wound and has better effect.

8. The extraction process provided by the invention obviously enhances the stability of chlorophyll, greatly improves the utilization rate, facilitates normal state storage, and can be popularized and used in a large range.

9. The device of the invention is not only convenient to operate, but also obviously improves the quality of chlorophyll, and is suitable for market popularization and use.

Drawings

None.

Detailed Description

In order that those skilled in the art can better understand the present invention, the following embodiments are provided to further illustrate the present invention.

The invention relates to a chlorophyll woundplast spraying bacteriostat liquid formula for diabetic gangrene, which comprises the following components:

aloe vera: 30 to 80 portions of

Folium artemisiae argyi: 50 to 100 parts of

Poplar leaf: 20 to 50 portions of

And (3) pagodatree leaves: 20 to 50 portions of

Fig leaves: 30 to 80 portions of

Corn: 80-180 parts.

Preferably, the aloe is 55 parts, the folium artemisiae argyi is 75 parts, the poplar leaves are 35 parts, the locust leaves are 35 parts, the fig leaves are 55 parts, and the corn is 130 parts. The design ensures that the diabetic wound can heal fastest and the curative effect is optimal.

Preferably, the aloe is 30 parts, the folium artemisiae argyi is 50 parts, the poplar leaves are 20 parts, the pagoda leaves are 20 parts, the fig leaves are 30 parts, and the corn is 80 parts. By adopting the design, the diabetic wound can be healed quickly, and the curative effect is better.

Preferably, the aloe is 80 parts, the folium artemisiae argyi is 100 parts, the poplar leaves are 50 parts, the locust leaves are 50 parts, the fig leaves are 80 parts, and the corn is 180 parts. By adopting the design, the diabetic wound can heal quickly and has better curative effect.

Preferably, the organic solvent in the first step is one or more of butanol, methyl chloride, petroleum ether, isopropanol, trichloroethylene, ethanol, acetone, dimethylformamide and dimethyl sulfoxide. The design has better leaching effect, can save the using amount of organic solvent and reduce the cost.

Preferably, the organic solvent in the first step is a 95% ethanol solution. The design has better leaching effect, can save the using amount of organic solvent and reduce the cost.

Preferably, the CGMCC0607 strain is inoculated to the culture medium at the temperature of 60-70 ℃, and dynamic culture is carried out at the temperature of 60-70 ℃. With the design, the treatment effect of the wound is better.

Preferably, after the dynamic culture is completed, 0.5 to 1.0 wt% of phenol and 0.5 to 1.0 wt% of diatomaceous earth are added to the culture system, followed by stirring and filtration. With the design, the treatment effect of the wound is better.

The formula of the invention has the following application range:

1. various skin injuries, acid and alkali burns and cold injuries caused by heat.

2. Various skin diseases, tinea, eczema, and toxic herpes.

3. Various traumas such as falling, beating, bumping, knife, gun, filing, bruising, blasting, etc.

4. Vaginitis, breast lumps.

5. Diabetic wounds, diseased feet.

6. Eye diseases such as acute contagious conjunctivitis, muscae volitantes, and asthenopia.

7. Alopecia areata.

8. For anal and intestinal diseases, internal and external hemorrhoids and bleeding due to dry fissures, it is applied internally and externally.

9. For urethritis and vaginitis, 5-10ml is injected into the product once a day.

10. The wound is not healed for a long time.

11. The cancer wound can also heal.

The use method of the formula comprises the following steps:

1. the untreated wound is sprayed with medicine continuously for several times, and when the red color of the wound is developed, the soaked skin is cut off, and the whole wound is covered with 4 layers of napkin paper or gauze. Spraying the medicinal liquid thoroughly, and fastening a layer of elastic silk cloth, such as sock leg.

2. The wound surface coated with other medicinal materials is cleaned with non-irritant 1-2% of the oil, and sprayed as 1 treatment.

3. The fistula wound surface is treated by connecting a soft tube with an injector, sucking 5ml of medicine, injecting for 2-3 times, and performing wet dressing and bandaging on the wound.

4. Necrotic tissue must be trimmed.

Changing the dressing: the burn time is within three days, dressing change is carried out every other day, and the dressing change is carried out every 3-4 days after the last three days. The dressing has no exudation, no swelling and pain around, no secretion overflow under light pressure, which indicates that the scab is fixed, the wound surface is not treated, the repeated diagnosis is carried out once in 7 days, and the repeated diagnosis is required after automatic scab removal. For example, the wound surface is deep red, and it is applied by wet compress.

If the dressing is completely wet, the whole dressing is removed, debridement is carried out, and the dressing is wrapped by No. 3 ointment.

If the dressing is partially infiltrated, only the dressing which is not dry is removed, the wound is cleared, and the dressing is wrapped by No. 3 ointment.

For example, after erosion and debridement, the powder of Sulfer-Mirabilitum is applied and the ointment No. 3 is applied for bandaging.

Treatment of acid-base burns:

spraying the product directly onto the new wound surface until the pH value is 7.0. Then the treatment is carried out according to the conventional operation.

The wound surface coated with other medicines must be debrided and then sprayed. (example: a crown burn with an alkaline burn, a muddy state for three months, a pH of 8.5)

The injured eyes are moved away to spray the solution until the pH value is neutral, and the solution is dropped into the eyes once within 2-3 hours.

The water-draining and inner-injecting method is adopted for the water bubbles on the soles and the palms. The preparation is applied once a day until the soaked skin is tightly adhered to the wound surface.

Unhealed wound, diabetic foot, bedsore, etc.

Non-healing wounds-wounds that do not heal for more than one month.

No. 1 debridement, No. 3 dressing change once a day or three days. (according to the amount of the wound exudate, the wound without exudate and dryness is not changed, and the wound is protected from scab until the wound is automatically decrusted)

When the rotten meat of the wound surface is not easy to remove, silver nitrate dry powder can be coated on the rotten meat for wrapping, the medicine is changed once in three days, the more brown liquid flows out, the better, and the more brown liquid flows out, the more brown liquid is squeezed and wiped off in time.

The medicine has no toxic and side effects, slightly stimulates eyes, does not hurt the eyes, has good affinity to anorectal tissues, is quickly absorbed by injected liquid medicine, is difficult to discharge, has no adverse effect on red blood cells, and is a repairman of a human body. (experiments per se) where the disease it is. (porphyrin compound has such a property)

The medicine has wide application and simple operation, and is a necessary medicine for battlefields, families, easy-to-hurt working surfaces, traveling and drivers.

Partial case extract

Mu a certain woman in the city of 55 years old

In 2 months and 15 days in 2009, the wound of the gynecological operation is 30cm long, 5cm wide and 5cm deep and is communicated with the abdominal cavity, and the wound is not healed because of the diabetes.

The product is applied after 3 months and 2 days, and the wound is completely healed and has no scar after 4 months and 1 day.

Luo someone male 70 years old Shijiazhuang

The gallstone is used for abdominal operation in four hospitals in Shishi city, the wound is 20cm long, and the diabetic can not heal due to wound suppuration, and the gallstone can completely heal after 20 days without scars.

Luo someone male 75 years old Shijiazhuang

The wounds of 15cm are respectively wound on both sides of the lower abdomen in the operation of the provincial hospital, the wounds can not be healed in 40 days, and the wounds can be healed in 15 days after the product is used.

Wu certain male 12 years old

In 09 years, 2 months, 14 pm, 4 am, 30 minutes, the right hand and the left wrist are burnt by gunpowder, III degrees and 2 percent of the right hand and the left wrist are burnt, and the patients live in a hospital to treat skin erosion and aggravation.

The product can be used for 2 months and 17 days to recover in 3 months and 21 days, and the hand veins are intact and no scar is healed.

Dong somewhere diabetes patient female 70 years old

The feet are scalded by boiling water at 7 o' clock in 3 months, 19 days and later, and the wounds are gradually worsened and eroded due to diabetes pathogens.

The product can be used after 3 months and 22 days, and can be completely recovered after 4 months and 20 days without scar.

40 years old Shijiazhuang city for a certain male in week

The molten alkali at 140 degrees at 11 o' clock and 30 min at 8 d night in 6 and 8 months in 08 years is exploded, and the alkaline water is poured throughout the whole body from the beginning and burns more than 95%.

After 1 hour, the product can be used for 7 months and 16 days without scar healing.

Luquan Liu somewhere

3, 31 days in 93 years, the right hand is extruded by a 240-degree dryer, and the whole hand becomes black, unconscious and necrotic after being treated for 18 days in a certain hospital, and is ready for amputation. The product is used in a trial attitude because of being unwilling to amputate, is completely recovered after 3 months, and solves the worldwide problem that the hand pattern cannot be regenerated.

Shijiazhuang Zhangqi

In 2002, the breast part of the No. 8, No. 14 right chest is burnt by flame gas welding, and after the breast is treated in a certain hospital for 19 days, granulation is aged and necrotized, and the breast is sunken by 0.5 cm. The product is used in hospitals after leaving the hospital for 20 days, and new nipples grow out and are intact as before.

Define any one of women, 50 years old, 10 months and 16 days 2008.

At 11 am on 26 months of 9, the head was squeezed, the ears were hung 90% from the head, and when the ear was sutured in a hospital in province and hospitalized, the ears gradually became black necrosis and had no feeling of needle prick. The product can be used for treating ear necrosis for two months.

Wangzhi man 2 years old

Sit on a red iron ring in the afternoon of 1 month and 8 months in 93 years.

And (4) checking: the buttocks and the inner side of the right thigh are scalded into brown by the iron ring and are peeled completely, and the burn area is 3 percent.

Treatment: spraying the liquid for treating burn after debridement. Pain was relieved within 15 minutes for three times. The preparation is administered 3-4 times daily without antibiotic.

After 1 month and 9 days, the inner side of thigh scabs, the buttocks scab, and the medicine is sprayed continuously.

After 1 month and 10 days, the scab skin is wiped off due to the contact of the buttocks, no infection is caused, and the medicine is sprayed on the exposed parts.

The scab is fixed on the buttocks for 1 month and 11 days, and the medicine is sprayed three times a day.

Spraying the medicine for 1 month and 12-15 days, wherein the scab feels itching.

The wound heals after 1 month and 16 days (most of scab is removed), and no scar is left.

The treatment course is 9 days.

Jia someone woman 32 years old

In 87 years, 5 and 9 days, the lower limbs are burnt by boiling oil carelessly, the area of II is about 20 percent, the patients do not improve after being treated in a certain provincial hospital for one month, and the patients are treated in 10 days in 6 months.

And (4) checking: t38 ℃, the wound surface is covered with a layer of gray and white rotten meat, the peculiar smell is obvious, the edema is obvious, the exudate is more, and the bleeding occurs in individual parts.

Treatment: and (5) debridement. An exposure therapy is taken. The Lu's burn liquid is smeared and sprayed for 3-5 times a day. At the same time, 160 ten thousand per day of penicillin was injected intramuscularly.

And 6, 17 days after 6 months, the rotten meat is removed, the bleeding and the edema disappear, the body temperature returns to normal, and the penicillin injection is stopped.

On 24 days after 6 months, scabbing began and itching appeared.

7 months and 1 day, the skin becomes ruddy after decrustation.

And 7, 8 days, the patient is healed to work.

After 8 months and 4 days, the legs are well healed by follow-up, and no scar, functional disorder and other adverse phenomena exist.

The treatment course is 28 days.

Luoyun 8 months old

In 89 years, 7 and 24 days, 10 am, half face, bladder, chest, back, pudendum and buttocks are scalded by boiled water. The patient is treated in Shijiazhuang air force hospital first, and the water bubbles are cut off. .

And (4) checking: t37.5 ℃, wound red, no more baby cry, II, area 37%.

Treatment: the wound surface is coated with Lushi burn liquid cotton ball, after 10 minutes, the infantile pain is stopped and the infant falls asleep, and the medicine is applied 3 times a day by adopting family ward treatment.

At noon of 7 months and 25 days, there was no exudate, the wound surface was dry, and the body temperature was normal.

After 7 months and 26 days, the scab begins on part, and the application method is changed into spraying, 6 times a day.

After 7 months and 30 days, all the wound surfaces enter the scabbing period, part of the wound surfaces begin to scab, the wound surfaces are good in spirit, free to play, normal in diet and erosive in pudendum, and special treatment is carried out.

8 months and 1 day, except for two blocks at the back and one block at the chest, just before decrustation. The other parts are all decrusted and show normal skin color. Dry and scab in the pudendum.

The scab on the back fell off after 8 months and 2 days.

And 8, 5 days after 8 months, the anterior chest crust skin is reduced from 12cm to 5 cm. The baby can climb up and lie freely.

After 8 months and 6 days, scabs all fall off, and the base of the injured part is slightly red.

The follow-up is carried out on 10 months and 1 day, all wound surfaces have no scars, the color of the wound surfaces is the same as that of normal skin, and no functional disorder exists.

The treatment course is 13 days.

Luo someone male 35 years old

3 am 3 pm in 3 months and 20 days in 91 years, when the automobile is repaired, the automobile is tempered and the face and hair are burnt by gasoline. The wound surface is red, swollen and painful.

The treatment is carried out 15 minutes after the burn is cured, LST wet dressing is adopted, pain is relieved within 45 minutes, no blister occurs, the wound surface is completely restored to normal secondary color after 2 hours, and the self-coating is carried out with LST50 ml.

And the patient is healed after 3 months and 21 days.

After 3 months and 25 days, a thin skin is removed without any trace.

56 years old king certain woman

At 11 o' clock in 91 years, 6.14.8.9.9.7.A safety valve of the pressure cooker fails, a cooker cover flies, and rice dumplings in the cooker completely fly out to scald the whole face, ears, lower jaw, neck, chest, back, upper limbs and shoulders, badger fat is applied at home, and a large amount of (more than one jin) alkaline flour is used for rubbing and sprinkling. The doctor visits 12 o' clock.

And (4) checking: the wound surface is dark red, the blister skin is shrunk, 3% of the wound surface III on the arm is adhered with a large amount of alkaline surface, and the wound surface is subjected to dry necrosis. Swelling of the face, intact skin. The mouth and eyes are not open, the nostrils are not open, and the body temperature is 38.5 ℃. The pH value of the wound surface is 14.0 (strong alkalinity).

Treatment: the wound surface was quickly rubbed with an LSY cotton ball once for 10 minutes, and after 2 hours, the face recovered normal secondary color. The body is washed with a large amount of normal saline, and then sprayed with LSY 6 times a day.

6 months and 15 days, T37.2 ℃, the wound surface is dry and does not exude, the edema of the eyelids and lips begins to subside, and the nostrils are unblocked. No pain and sleep quietly.

After 6 months and 17 days, the edema of mouth, nose and eyes disappears. III, the wound surface is dry and sunken, and the key treatment is performed.

6 months and 19 days, the scab skin in the nose is removed and cut off, the face begins to scab, and the dry dead face is improved.

And after escharosis is finished on the face and the back after 6 months and 24 days, the normal skin color is recovered without scars, a thin layer of skin is peeled off from the lips, escharosis starts on the chest, III slight infection caused by eschar skin abrasion of the wound surface is achieved, and LSY wet dressing is performed.

After 28 days in 6 months, the crusts of the neck and the chest completely fall off, the skin color is normal, and no scar is left.

And in 8 months and 2 days, the wound surface of the right arm III is healed and healed after 45 days without skin grafting. Has no scar left. The treatment course includes 14 days II wound surface and 46 days III wound surface.

42 years old of a certain male

The patient falls into an alkali burning pool after 7 and 23 days in 92 years, is washed by tap water and then has a bath, the lower limbs and the abdomen are protected, the scrotum skin falls off, a large amount of serous fluid seeps out, toilet paper needs to be changed once per hour, the patient can see a doctor quickly in a certain hospital, the doctor orders to perform the doctor, the patient can blow the patient by using an electric fan after the patient opens the legs, the patient is extremely painful, and the patient can see the doctor before 26 days in 7 and 26 days.

And (4) checking: erosion of scrotum and severe exudation of serous fluid.

Treatment: the wound surface was dried for 8 hours and exudation was stopped by treatment with LSY spray.

Scabbing began on day 7, day 27.

After 8 months and 4 days, the skin is removed by one layer, and the skin is healed without scars.

The treatment course is 9 days.

Luo someone male 3.5 years old

Sitting in the pan with the boiled surface for 12 months and 16 months in 92 years, 6 pm, scald the buttocks, perineum, external genitalia and anus, and once coated with a layer of toothpaste. Visit 7 o 'clock and 15 o' clock before the clinic.

And (4) checking: the wound surface is red and swollen, most of large blisters exist, the root of the leg is soaked and peeled off, the blisters around the testis, the external genitalia and the anus are connected into a whole, the glans penis is large in edema like walnut, and the burn area is 10% II.

Treatment: discharging the liquid in the bubbles, spraying LSY20 min, stopping pain and crying, and carrying out self-spraying 6 times a day.

After 12 months and 17 days, the buttocks, the anus and the root of the leg start to fix scabs, the red and swollen wound surface at the edge of the blister recovers the normal skin color, and the left thigh carelessly generates adhesion infection and slightly oozes when sleeping at night. The swollen penis still can urinate with a slight pain.

12 months and 18 days, two legs are treated by poor nursing, infection and wet compress.

12 months and 20 days, residual small blisters on the legs are removed, glans edema is relieved, the two legs are still eroded, and debridement and wet dressing are carried out.

After 12 months and 21 days, the buttocks are decrusted and healed, the penile edema disappears, wrinkles begin to appear, the left leg has small-area inflammation at the root, and the wet compress treatment is carried out.

12 months and 23 days, the wound infection is totally controlled.

12, 24 days, the wound is in decrustation stage, and the wound of penis, testis and anus is recovered to normal.

After 12 months and 30 days, except that a large thick crust of the walnut is not peeled off at the hip, the wound surfaces are all decrusted, the normal skin color is recovered, and the wound surface is smooth, flat and scarless, so that the child can jump freely on the bed.

The treatment course is 14 days.

Song some male 22 years old

And 3, 12 days in 93 years, when the cap of the concentrated nitric acid bottle is started, the concentrated nitric acid is carelessly splashed into the right eye, and the red and swollen pain and the pain are timely treated after the water washing.

And (4) checking: the upper and lower eyelids of the right eye are red and swollen. Transparent, with the lower eyelid heavier than the upper eyelid, the eye is difficult to open.

Treatment: the LSY50ml was self-applied after being applied by wet dressing with mild stinging and pain-relieving for a short time, the edema disappeared apparently after 20 minutes, and the eyes opened automatically (no injury was noticed).

The disease heals in 3 months and 13 days.

The treatment course is 2 days.

The year 55 of a male from charge

And 3, after the patient is electrified, the positive electrode and the negative electrode are collided to generate electric arcs when the patient is electrified, the body is shot 2 meters away, a plurality of parts of the face and the hands are burnt, and the patient can see a doctor in time after being soaked in cold water.

And (4) checking: burns on the face, cheeks, thumbs of the right hand, the food on the left hand, the middle thumb, the thumb and the back of the hand are scorched, edema and severe pain.

After 8 months and 19 days, the edema of the wound surface disappears, the wound surface has no pain and numb feeling, and the wound is wrapped by wet dressing.

After 8 months and 20 days, the dry skin of the left thumb is cracked, and the left thumb is wrapped by wet dressing.

And on day 8, month 22, peeling, and wet dressing.

After 8 months and 24 days, the left index finger eschar, the right thumb heals (the eschar survives and does not break away), and the left thumb has slightly swollen alkali skin and is applied by wet compress.

After 8 months and 25 days, the left middle finger heals and moves freely.

And the disease is cured after 8 months and 26 days.

The treatment course is 8 days.

41 years old Zhao certain man

The tractor stands up at 5 and 14 days in 93 years, boiling water is poured on two legs, the wound surface is not healed when the patient is treated in a county hospital for 47 days, and the patient is treated in 1 day in 7 and 7 months in 93 years.

And (4) checking: the burn area is 20%, the wound surface is not healed by burn III, the wound surface is erosive from the inner side of the thigh to the knee, and other wound surfaces are purplish red and sometimes generate pustule.

Treatment: the wound surface was cleaned with LSY and sprayed 6 times daily.

In 7 months and 2 days, the erosion wound surface is obviously reduced.

In 7 months and 3 days, the erosion wound is changed into wet dressing.

7, 6 days, the wound surface is better and smooth, and the erosion surface is strengthened by wet dressing.

After 7 months and 10 days, the wound surface has itching feeling, the wound surface is obviously improved, and the wet dressing is continued.

Spraying LSY six times a day for 7 months and 11 to 30 days without special condition.

In 8 months and 1 day, the erosion face is completely healed without scar formation.

The treatment course is 30 days.

76 years old of somebody high

The etiology is as follows: diabetic gangrene. In 1999, the left toe was cut off for a short time, and the right foot was cut off with one toe. In 2000, the first segment of the right foot, 2, 3, 4 toes, necrosed and blackened.

The healing time is from 1 month and 2 days to 6 months and 30 days in 2000, and the normal color is recovered.

Old male 11 years old

Blasting and blasting injury, and burning right hand III.

The disease heals within a period of days from 24 days of 1 month to 28 days of 1 month in 2000.

Extremely A woman 65 years old

She was again 4 plus diabetic patients.

The rear part of the right foot is cut by spokes of a bicycle, and after the whole skin layer falls off and is treated for three days in a certain hospital, a large amount of blood secretion is generated on the wound surface, and the tissue is pale and black.

The wound surface heals in 26 days in 4 months to 6 days in 7 months in 2000, and the walking is normal.

Jia somebody male 20 years old

The burning of caustic soda, pale in the face and pupils of both eyes, does not allow viewing of the object.

The treatment can be carried out in 2002 for 1 month and 9 days to 1 month and 27 days without scars. The vision of both eyes is recovered to be normal.

50 years old for a certain male

The right chest and breast are burned by gas welding flame, and after being treated for 19 days in a certain hospital, the granulation is aged and necrotic and is sunken by 0.5 cm.

Treatment: the disease heals in 2002 in 8, 14 to 9, 4 days and new nipples grow.

30 years old male of Tian Yi

Due to the fact that the burn caused by fire of gunpowder is 85% in handling of the mortuary works, a patient is in a critical notice after the gunpowder is hospitalized in a certain hospital, then the patient is discharged and transferred to a local health hospital, the patient is cured from 7 months and 30 days to 9 months and 25 days in 96 years by using the gunpowder, only slight scars are left, the function is normal, and the patient can be recovered to be put on the shelf by the former masons.

38 years old female in forest

Burning with sulfuric acid to form III burns on both lower limbs, left arm and palm, and grafting skin and amputation after examination in a hospital. After the treatment of the medicine, only mild scars are left, and the medicine has no dysfunction and is normally on duty.

Zhangzhi man 40 years old

Electric shock injury: the first section of the right hand, the thumb, the inner side of the first section of the ring finger, the inner and outer sides of the first section of the middle finger of the right hand, the inner sides of the 2 and 3 sections of the index finger, and the first section of the ring finger respectively have burn, white and necrosis wound surfaces of 1-3cm and 3mm in depth, and the wound surfaces begin to erode and are unconscious after 5 days of hospital treatment.

The medicine can be cured after 9/8-12/21 days in 99 years, and can be recovered to normal state without scar.

38 years old male king

The anal fistula has operated for a plurality of times to form a deep hole with three incisions with the depth of 10 cm.

Treatment: the medicine can be cured in 998 years, 11 months and 13 days to 12 months and 21 days, and the medicine can be recovered to normal without scars.

42 years old somebody a plum

Balanus erosion, bleeding and itching.

Treatment: the disease can be cured in 9 and 6 days in 99 years and in 10 and 7 days. The function is normal.

Hooked girl 31 years old

The main symptoms are: systemic lupus erythematosus.

Fumigation treatment of the bent side of the right shank of a patient with a burn and scald of 4 x15cm

Necrosis depth of 1.5cm

The treatment can be carried out on 27 days 8-4 days 10 in 2000 without scars, and the lupus erythematosus of the female is cured, and the female becomes a beautiful woman with a plump red light and full face by bones as thin as firewood.

Stone A male age of 31 years

Liquid ammonia, sprayed on face, with ammonia toxicity and frostbite

Treatment of 2002-11/25/11/29/day

Healing without scar left.

42 years old someone Liu

Hot-pressing injury: the right hand is flaked and black, and is unconscious and requires amputation.

After three more months of treatment, the normal function is recovered, and the bucket weighing 30 jin can be lifted.

Song certain woman 50 years old

Bedsore: wound surface of sacrum 12cm, depth 10mm, and inner sleeve 1-1.5 cm

Treatment: the paste can be healed in 10 and 6 days in 99 years and 28 days in 11 months without scars.

Luquan Liu Gong

The right hand is squeezed by a 240-degree dryer, the whole hand is unconsciously necrotic, and amputation is required by other hospitals once, and the limb is cured after 100 days of treatment in our places.

Zhuang city of Shi Jia

The right breast is burnt by flame of gas welding, granulation necrosis, 0.5cm concave, and can be cured after 20 days of treatment.

Shijiazhuang city Gao

Diabetic gangrene and toe necrosis can be cured after the treatment.

Some Gao Cheng Fang

The wound after gynecological operation is 30 x 50 x 5cm, and is not healed for a long time due to diabetes. After one month of treatment, no scar is healed.

Shijiazhuang city luqi

After the operation, the wound of 15cm is respectively arranged on the two sides of the lower abdomen, the lower abdomen can not be healed for a long time, and the lower abdomen can be healed without scars after 15 days of treatment.

Shijiazhuang city Zhou-chi

Is watered with 140 ° molten alkali water to burn more than 90%. After one month, the medicine can heal without scar.

Positively define any one of

The head is injured due to car accidents, the ears are hung from the head by 90 percent, the whole ears are blackened and necrotized after the suture treatment in a certain hospital for half a month, and then the ears are turned to the same place to be cured after the treatment for two months.

Shijiazhuang city Dong (a kind of stone)

The feet are scalded by boiled water, and the wounds of the diabetics gradually worsen and erode, so that the wounds can heal without scars after one month of treatment.

Shijiazhuang city von

The palm is exploded through the gun in the year, the whole hand loses consciousness, and the disease can be cured without scars after two months of treatment.

Zhao county high

Because the wound from the knee to the ankle of the left leg is 30 x 10 x 13cm and is not healed for a long time after the automobile accident operation, the hospital requires amputation and then turns to the place to be healed after 3 months of treatment.

Mr. May 85 years old Luoyang

The main reasons are as follows: non-healing after diabetic gangrene operation

The current medical history: in 2018, 12 and 25 months, diabetic gangrene is amputated in a hospital, and the wound continues to be ulcerated after the operation. The blood sugar of the patient is about 11 points, and the Alzheimer disease is caused.

Wound wet dressing of chlorophyll starting at 1 month and 1 day of 2019

History of the past: diabetes, Alzheimer's disease

And (3) diagnosis: non-healing after diabetic gangrene operation

The treatment process comprises the following steps: wound-cleaning and wet-dressing chlorophyll

Diabetic gangrene treatment procedure

The patients: lu Mr. 78 years old Shi Zhuang ren

The main reasons are as follows: gangrene due to diabetes

The current medical history:

diabetes mellitus is for over a decade. The wet gangrene of the right foot has had amputation of the big toe, and the wound continues to worsen and faces secondary amputation. The whole foot is dark purple and has unsmooth blood circulation. Dry gangrene of the left foot and the three toes. After one month of chlorophyll application, new granulation of the right foot grows out and heals up by 80%. The left foot is healed, the patient hears a month with excellent effect, and the chlorophyll is privately stopped to use other medicines. The face 7.26 was photographed for 7.26 days, and the wound was worsened and ulcerated by 80% healing on 6.23 days. Photograph 8.17 is 7.26 since the product was used newly after relapse. Clapping for 9.5 days, and growing the sunken part flatly to wait for automatic decrustation.

The treatment process comprises the following steps: debridement, and wet application of chlorophyll.

The treatment time is as follows: 2018.5.26-2018.6.23

Patients' meng, men, age 74, ning jin,

the main reasons are as follows: no healing was achieved after 2 months after post-cervical post-bulkiness incision, and diagnosis was given at 2019.5, 13.

The current medical history: the disease onset time begins 2 months in 2019, multiple small pustules are generated behind the neck, repeated swelling and pain are caused, the disease is not easy to cure and gradually worsened when a plurality of local patients are treated, and the swollen and pain is aggravated along with fever in 2019.4.13 Hospital of Shijiazhuang city, so that the swollen and swollen neck and diabetes mellitus are diagnosed. The treatment conditions such as incision and drainage through abscess, control of hyperglycemia and the like are obviously improved, but the wound is cured slowly.

Visit a community at 2019.5.13, local wound examination: the incision and drainage wounds of the abscess positioned behind the neck are respectively 6cm, 5cm and 3cm in size, the subcutaneous sore cavity range is about 20x15cm, cream yellow viscous pus is filled in the incision and drainage wounds, no obvious peculiar smell exists, surrounding tissues are red, swollen and thickened, and dispersed hair follicle purulent vesicles exist behind the pillow and the surrounding skin. 8.4mmol/L of fasting blood sugar and 9.8mmol/L of fasting blood sugar after 2 hours.

History of the past: healthy, without history of hypertensive coronary heart disease and diabetes.

And (3) diagnosis: 1. postjugular abscess incision drainage (Staphylococcus aureus and streptococcus mixed infection)

Type II diabetes

The treatment process comprises the following steps: 1. oral medicine for controlling blood sugar

2. Local chlorophyll dressing change

3. Local anesthesia descending debridement and plastic surgery

The following tests were carried out on the product produced by the invention:

the detection items are as follows: bacterial colony total number, fungal colony total number, coliform group, staphylococcus aureus, pseudomonas aeruginosa, hemolytic streptococcus, antibacterial property, pH value, arsenic, lead, mercury, active ingredient polyhexamethylene biguanide content and stability test thereof

1. Microbiological indicator detection

The detection results of the items detected by the sample, such as coliform bacteria, pseudomonas aeruginosa, staphylococcus aureus, hemolytic streptococcus, total bacterial colonies and total fungal colonies, meet the requirements of GB 15979-.

2. Bacteriostatic property

The sample is diluted by 10 times, the action time is 2 minutes, the test is repeated for 3 times, the bacteriostasis rate to escherichia coli (ATCC25922), staphylococcus aureus (ATCC 6538) and candida albicans (ATCC 10231) reaches the standard value (the bacteriostasis rate is more than or equal to 90 percent) of GB 15979 annex C4, and the sample has stronger bacteriostasis to the tested strain.

pH value test:

the pH value (25 ℃) of the chlorophyll woundplast bacteriostatic agent of the sample is 6.58.

4. Heavy metal test:

the arsenic content of the chlorophyll woundplast bacteriostat liquid of the sample is less than 0.05mg/kg, the lead content is less than 0.05mg/kg, the mercury content is less than 0.05mg/kg, and the detection result of the detected item meets the standard requirement of technical specification for cosmetic safety (2015 edition).

5. Polyhexamethylene biguanide content test:

the content of polyhexamethylene biguanide in the chlorophyll woundplast bacteriostatic solution of the sample is 0.0172 percent.

6. And (3) stability test:

the initial content of polyhexamethylene biguanide in the sample was measured to be 0.0172%. After the sample is stored for 14 days at 54 ℃, the content of the polyhexamethylene biguanide is 0.0168 percent, the reduction rate is 2.3 percent compared with that before the sample is stored, the requirement that the reduction rate of the effective components in the technical specification of disinfection (2002 edition) -2.2.3.2.1 is not more than 10 percent is met, and the storage life of the sample can be set as 1 year.

In summary, the detection conclusion is as follows:

1. and (3) microorganism index detection, wherein the detection results of the items detected by the sample, such as coliform bacteria, pseudomonas aeruginosa, staphylococcus aureus, hemolytic streptococcus, total bacterial colonies and total fungal colonies, meet the requirements of GB 15979-.

2. The sample is diluted by 10 times, the action time is 2 minutes, the test is repeated for 3 times, the bacteriostasis rate to escherichia coli (ATCC25922), staphylococcus aureus (ATCC 6538) and candida albicans (ATCC 10231) reaches the standard value (the bacteriostasis rate is more than or equal to 90 percent) of GB 15979-2002-annex C4, and the sample has stronger bacteriostasis to the tested strain.

And 3, pH value test, namely the pH value (25 ℃) of the chlorophyll woundplast bacteriostatic agent of the sample is 6.58.

4. Heavy metal test, wherein the arsenic content of the chlorophyll woundplast bacteriostatic solution is less than 0.05mg/kg, the lead content is less than 0.05mg/kg, the mercury content is less than 0.05mg/kg, and the detection result of the detected item meets the standard requirement of technical safety standards of cosmetics (2015 edition).

5. In the content test of the polyhexamethylene biguanide, the content of the polyhexamethylene biguanide in the chlorophyll woundplast bacteriostatic solution of the sample is 0.0172 percent.

6. Stability test initial content of polyhexamethylene biguanide in the sample was determined to be 0.0172%. After the sample is stored for 14 days at 54 ℃, the content of the polyhexamethylene biguanide is 0.0168 percent, the reduction rate is 2.3 percent compared with that before the sample is stored, the requirement that the reduction rate of the effective components in the technical specification of disinfection (2002 edition) -2.2.3.2.1 is not more than 10 percent is met, and the storage life of the sample can be set as 1 year.

Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.

Claims

1. The chlorophyll spraying bacteriostatic solution formula for diabetic gangrene is characterized by comprising the following components:

aloe vera: 30 to 80 portions of

Folium artemisiae argyi: 50 to 100 parts of

Poplar leaf: 20 to 50 portions of

And (3) pagodatree leaves: 20 to 50 portions of

Fig leaves: 30 to 80 portions of

Corn: 80-180 parts.

2. The chlorophyll woundplast bacteriostatic formulation for diabetic gangrene according to claim 1, characterized in that: 55 parts of aloe, 75 parts of folium artemisiae argyi, 35 parts of poplar leaves, 35 parts of sophora leaves, 55 parts of fig leaves and 130 parts of corn.

3. The chlorophyll woundplast bacteriostatic formulation for diabetic gangrene according to claim 1, characterized in that: the aloe tea is prepared from 30 parts of aloe, 50 parts of folium artemisiae argyi, 20 parts of poplar leaf, 20 parts of sophora leaf, 30 parts of fig leaf and 80 parts of corn.

4. The chlorophyll woundplast bacteriostatic formulation for diabetic gangrene according to claim 1, characterized in that: the aloe tea is prepared from 80 parts of aloe, 100 parts of folium artemisiae argyi, 50 parts of poplar leaf, 50 parts of sophora japonica leaf, 80 parts of fig leaf and 180 parts of corn.

5. The process for extracting chlorophyll woundplast spray bacteriostat formulation for diabetic gangrene according to any one of claims 1 to 4, comprising the steps of:

6. The extraction process according to claim 5, characterized in that: the organic solvent in the first step is one or more of butanol, methane chloride, petroleum ether, isopropanol, trichloroethylene, ethanol, acetone, dimethylformamide and dimethyl sulfoxide.

7. The extraction process according to claim 6, characterized in that: the organic solvent in the first step is 95% ethanol solution.

8. The process for extracting chlorophyll woundplast spray bacteriostat formulation for diabetic gangrene according to any one of claims 1 to 4, comprising the steps of:

9. The extraction process according to claim 8, characterized in that: inoculating CGMCC0607 strain to the culture medium at the temperature of 60-70 ℃, and dynamically culturing at the temperature of 60-70 ℃.

10. The extraction process according to claim 9, characterized in that: after the dynamic culture is finished, 0.5 to 1.0 weight percent of phenol and 0.5 to 1.0 weight percent of diatomite are added into the culture system, and then the mixture is stirred and filtered.