CN111812086A - Trisodium trithiocyanate rapid detection test paper and preparation method and detection method thereof - Google Patents
Trisodium trithiocyanate rapid detection test paper and preparation method and detection method thereof Download PDFInfo
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- BYGYBSHPLSVNGL-UHFFFAOYSA-K trisodium trithiocyanate Chemical compound [Na+].[Na+].[Na+].[S-]C#N.[S-]C#N.[S-]C#N BYGYBSHPLSVNGL-UHFFFAOYSA-K 0.000 title claims abstract description 104
- 238000001514 detection method Methods 0.000 title claims abstract description 61
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- 238000004587 chromatography analysis Methods 0.000 claims description 13
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 11
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- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 8
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Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/10—Starch-containing substances, e.g. dough
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
Abstract
The invention relates to trisodium trithiocyanate rapid detection test paper, a preparation method and a detection method thereof2+、Fe2+、Sn2+、Ni2+Any one of the above color developing agents, further comprising a thickener in an amount of 0.1 to 2% by mass. The preparation method comprises the preparation of a color developing agent and the preparation of color developing test paper. The test paper for rapidly detecting the trisodium trithiocyanate, which is prepared by the invention, has the advantages of simple manufacturing method and low cost, can rapidly and highly sensitively detect the trisodium trithiocyanate in a sample, is suitable for rapidly screening a large number of samples, is convenient to carry, transport, store and use, is convenient for on-site detection, and is also convenient for civil self-inspection.
Description
Technical Field
The invention relates to the technical field of food detection, in particular to trisodium trithiocyanate rapid detection test paper and a preparation method and a detection method thereof.
Background
Trisodium trithiocyanate (TMT-Na) is a high-efficiency and rapid rubber vulcanizing agent and is widely applied to vulcanizing agents of acrylate rubber, polychlorinated ether elastomers, chloroprene rubber and the like. In addition, TMT-Na is an environment-friendly heavy metal ion remover, can be chelated with most of monovalent and divalent heavy metal ions in an aqueous solution to form a stable compound which is difficult to dissolve in water, has good chemical stability, and is widely used for removing heavy metal ions in sewage and wastewater. TMT-Na has a reactive thiol group and can participate in the formation of disulfide bonds between mucedin. In recent years, illegal vendors have pursued the interest of using TMT-Na in the preparation of a compound flour treatment and adding it to flour to improve the flour quality of the flour. However, TMT-Na has a certain corrosivity, has an eye-stimulating effect, and has moderate toxicity (LD)50Mice 878mg/kg per mouth), and TMT-Na is not listed in the food additive list of GB2760-2014 national food safety Standard food additive use Standard. The illegal addition of TMT-Na in the compound flour treating agent not only threatens the health of consumers, but also has adverse effect on the safety of the flour industry in China.
At present, the general administration of market supervision has issued a notice of a food supplement inspection method of 'determination of trisodium trithiocyanate in wheat flour', the detection method used in the method is liquid chromatography, and the method has the advantages of complex operation, long inspection time, large waste liquid generation amount, higher technical requirement on operators, expensive detection instruments, high detection cost and fixed place requirement. TMT-Na in the wheat flour and flour treating agent can not be detected by field sampling inspection or civil self-inspection, which brings great inconvenience to people.
Disclosure of Invention
The invention provides trisodium trithiocyanate rapid detection test paper, a preparation method and a detection method thereof, and aims to solve the problems of high cost, long time consumption, expensive instruments and the like of the detection method of trisodium trithiocyanate in the existing flour or flour treating agent.
The technical scheme adopted by the invention is as follows: the test paper comprises a standard colorimetric card and a color development test paper, wherein the color development test paper is prepared by infiltrating a color development agent, the color development agent comprises a saturated inorganic metal salt solution, and metal ions of the saturated inorganic metal salt solution are selected from Cu2+、Fe2+、Sn2+、Ni2+Any one of the above.
Preferably, the color developing agent also comprises a thickening agent with the mass fraction of 0.1-2%.
Preferably, the developer comprises saturated CuSO4The solution and a thickening agent, wherein the thickening agent is one or a mixture of two of sodium carboxymethyl cellulose and hydroxypropyl methyl cellulose.
Preferably, the color test paper is selected from any one of filter paper and rice paper.
Preferably, the chromogenic test paper is selected from slow chromatography qualitative analysis filter paper.
The preparation method of the trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
s1: preparing a color developing agent: adding an inorganic metal salt reagent into distilled water for multiple times, fully stirring until the inorganic metal salt reagent is not dissolved any more to prepare a mixed solution, and filtering the mixed solution by using quick chromatography qualitative filter paper to obtain a saturated inorganic metal salt solution;
s2: preparation of color test paper: soaking uncut filter paper or rice paper in a color developing agent at normal temperature, soaking at room temperature for 10s, taking out, airing and cutting into a proper shape to obtain the color developing test paper.
Preferably, the preparation method of the color developer further comprises the following steps: and (4) adding the corresponding thickening agent into the inorganic metal salt solution prepared in the step (S1), heating and stirring until the thickening agent is completely dissolved, and cooling to room temperature for later use.
Preferably, the preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: and (4) dripping the standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, and soaking and airing the color development test paper by using a color development agent to obtain the sodium trithiocyanate solution.
W3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
A detection method of trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
q1: preparation of a solution to be tested: adding 1ml of flour or flour treating agent into a mixing container, adding 3ml of distilled water into the mixing container, stirring, and filtering by a filter head to obtain a solution to be detected;
q2: and (3) testing: and (3) taking a drop of the solution to be detected prepared in the step Q1, dropping the drop of the solution to be detected in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of trisodium trithiocyanate in the solution to be detected.
Preferably, the mixing container described in the step Q1 is a 5ml medical syringe, the end of the nipple of the medical syringe is sleeved with an aqueous or organic filter head with a pore diameter of not more than 0.45 μm, the piston end of the medical syringe is pulled out, 1ml of flour or flour treatment agent is added into the empty cylinder of the medical syringe, the piston of the medical syringe is pushed to make the front end of the piston at the 1ml position, then 3ml of distilled water is sucked and 1-2ml of air is subsequently sucked, the medical syringe is vigorously shaken to mix the solutions therein, and the piston of the syringe is pushed to discard 2-3 drops of filtrate at the end of the syringe.
Compared with the prior art, the invention has the following advantages: the test paper for rapidly detecting the trisodium trithiocyanate, which is prepared by the invention, has the advantages of simple manufacturing method and low cost, can rapidly and highly sensitively detect the trisodium trithiocyanate in a sample, does not generate organic waste liquid, is suitable for rapidly screening a large number of samples, is convenient to carry, transport, store and use, facilitates field detection, and is convenient for civil self-detection.
Drawings
FIG. 1 is a schematic diagram of the detection results of the chromogenic test paper in examples 1 and 6 of the present invention;
FIG. 2 shows the amount of water added for different TMT-Na concentrations in the flour;
FIG. 3 is a graph of corrected water uptake for different TMT-Na concentrations in flour;
FIG. 4 is a graph of the formation time for different TMT-Na concentrations in flour;
FIG. 5 is a graph of the stabilization time for different TMT-Na concentrations in flour;
FIG. 6 is the degree of softening at different TMT-Na concentrations in the flour;
FIG. 7 shows the extensibility at different TMT-Na concentrations in the flour;
FIG. 8 is a graph of tensile resistance at different TMT-Na concentrations in flour;
FIG. 9 is the maximum resistance at different TMT-Na concentrations in the flour;
fig. 10 is a standard colorimetric card according to the present invention.
Detailed Description
The contents of the present invention can be more easily understood by referring to the following detailed description of preferred embodiments of the present invention and examples included therein, and unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs, and in the case of contradiction, the definitions in the present specification shall control. The term "prepared from … …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "meaning," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
The conjunction "consisting of … …" excludes any unspecified elements, steps or components. If used in a claim, this phrase is intended to claim as closed, meaning that it does not include materials other than those described, except in connection with the common journal of usage. When the phrase "consisting of … …" appears in a clause of the claimed subject matter rather than immediately following the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of values, with an upper limit preferred value and a lower limit preferred value, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.
Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.
In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.
In order to solve the technical problems, the invention provides, in a first aspect, a trisodium trithiocyanate rapid test paper, which includes a standard colorimetric card and a color development test paper, wherein the color development test paper is prepared by infiltrating a color development reagent, the color development reagent includes a saturated inorganic metal salt solution, and metal ions of the saturated inorganic metal salt solution are selected from Cu2+、Fe2+、Sn2+、Ni2+Preferably, Cu is contained2+The inorganic metal salt solution is selected from saturated CuSO4Solution of Fe2+The inorganic metal salt solution is selected from saturated FeCl2Solution of Sn2+The inorganic metal salt solution is selected from saturated SnCl2Solution of Ni2+The inorganic metal salt solution is selected from saturated NiCl2The solution, when these metal ions are coordinated with the organic developer trisodium trithiocyanate, may form a complex that has a characteristic color and is thus displayed on the color test paper.
In these saturated inorganic metal solutions, CuSO is due to saturation4The solution has the best color development effect, therefore, the inorganic metal solution is preferably saturated CuSO4And (3) solution.
In order to improve the color development effect, the color development agent also comprises a thickening agent with the mass fraction of 0.1-2%, and preferably, the thickening agent is selected from one or a mixture of two of sodium carboxymethyl cellulose and hydroxypropyl methyl cellulose.
Further, the color development test paper is selected from any one of filter paper and rice paper, and is preferably slow chromatography qualitative analysis filter paper.
The second invention provides a preparation method of trisodium trithiocyanate rapid detection test paper, which at least comprises the following steps:
s1: preparing a color developing agent: adding an inorganic metal salt reagent into distilled water (the distilled water in the invention is water meeting the requirements of GB/T6682-;
s2: preparation of color test paper: soaking uncut filter paper or rice paper in a color developing agent at normal temperature, soaking at room temperature for 10s, taking out, airing and cutting into a proper shape to obtain the color developing test paper.
Further, in order to improve the color development effect, the color developing agent also comprises a thickening agent, and the preparation method comprises the following steps: and (4) adding the corresponding thickening agent into the inorganic metal salt solution prepared in the step (S1), heating and stirring until the thickening agent is completely dissolved, and cooling to room temperature for later use.
The preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% (mass concentration) of standard trisodium trithiocyanate aqueous solution;
w2: and (4) dripping the standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, and soaking and airing the color development test paper by using a color development agent to obtain the sodium trithiocyanate solution.
W3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
The third invention provides a detection method of trisodium trithiocyanate rapid detection test paper, which at least comprises the following steps:
q1: preparation of a solution to be tested: adding 1ml of flour or flour treating agent into a mixing container, adding 3ml of distilled water into the mixing container, stirring, and filtering by a filter head to obtain a solution to be detected;
q2: and (3) testing: and (3) taking a drop of the solution to be detected prepared in the step Q1, dropping the drop of the solution to be detected in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of trisodium trithiocyanate in the solution to be detected.
Further, the mixing container described in the step Q1 is a 5ml medical syringe, the end of the nipple of the medical syringe is sleeved with a water-based or organic filter head with a pore diameter of not more than 0.45 μm, the piston end of the medical syringe is pulled out, 1ml of flour or flour treatment agent is added into the empty cylinder of the medical syringe, the piston of the medical syringe is pushed to make the front end of the piston located at the 1ml position, then 3ml of distilled water is sucked and 1-2ml of air is subsequently sucked, the medical syringe is vigorously shaken to mix the solutions therein, and the piston of the syringe is pushed to discard 2-3 drops of filtrate at the end of the syringe. Through setting up water system or organic system filter head can prevent that flour from dripping from medical syringe after adding water, the setting of this filter head has avoided some impurity influences the detection effect in the solution that awaits measuring simultaneously for the solution that awaits measuring is clear transparent solution, guarantees the accuracy that detects.
The present invention will be specifically described below by way of examples. It is to be noted that the following examples are only for further illustration of the present invention and should not be construed as limiting the scope of the present invention. Many non-essential modifications and adaptations of the present invention will occur to those skilled in the art in view of the foregoing description, and are intended to be within the scope of the present invention. In addition, the starting materials used are all commercially available, unless otherwise specified.
Example 1
The test paper comprises a standard colorimetric card and a chromogenic test paper, wherein the chromogenic test paper is prepared by infiltrating a chromogenic agent, and the chromogenic agent comprises saturated CuSO4The solution and 0.5 percent of sodium carboxymethyl cellulose by mass fraction, and the chromogenic test paper is selected from slow chromatography qualitative analysis filter paper.
The preparation method of the trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
s1: preparing a color developing agent: adding CuSO into 100ml distilled water for several times4Stirring the reagent fully until the reaction solution is CuSO4Preparing a mixed solution after the reagent is not dissolved any more, and filtering the mixed solution by using quick chromatography qualitative filter paper to obtain saturated CuSO4A solution; to saturated CuSO4Adding corresponding sodium carboxymethylcellulose into the solution, heating and stirring until the sodium carboxymethylcellulose is completely dissolved, and cooling to room temperature for later use.
S2: preparation of color test paper: and (3) soaking uncut slow chromatographic qualitative analysis filter paper in a color developing agent at normal temperature, soaking at the room temperature for 10s, taking out, airing and cutting into a proper shape to obtain the color developing test paper.
The preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: and (4) dripping the standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, and soaking and airing the color development test paper by using a color development agent to obtain the sodium trithiocyanate solution.
W3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
A detection method of trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
q1: preparation of a solution to be tested: taking a 5ml medical injector, sleeving a water-based or organic filter head with the aperture not more than 0.45 mu m at the end part of a nipple of the medical injector, pulling out a piston end of the medical injector, adding 1ml of flour or flour treating agent into an empty cylinder of the medical injector, pushing the piston of the medical injector to enable the front end of the piston to be positioned at a 1ml position, then sucking 3ml of distilled water and then 1-2ml of air, violently shaking the medical injector to uniformly mix the solution in the medical injector, and pushing the piston of the injector to discard 2-3 drops of filtrate at the end part of the injector.
Q2: and (3) testing: and (3) dripping the solution in the traditional Chinese medicine injector in the step Q1 in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of the trisodium trithiocyanate in the solution to be detected.
Example 2
The test paper comprises a standard colorimetric card and a color development test paper, wherein the color development test paper is prepared by infiltrating a color development agent, and the color development agent comprises saturated FeCl2The solution and the color test paper are filter paper.
The preparation method of the trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
s1: preparing a color developing agent: FeCl was added in portions to 100ml of distilled water2The reagent is fully stirred until FeCl2The reagent is not dissolved any more to prepare a mixed solution, and the mixed solution is filtered by a rapid chromatographic qualitative filter paper to obtain saturated FeCl2A solution;
s2: preparation of color test paper: and (3) soaking uncut filter paper in a color developing agent at normal temperature, taking out and airing after soaking for 10s at the room temperature, and cutting into a proper shape to obtain the color developing test paper.
The preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: dripping standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, wherein the color development test paper is obtained by soaking treatment through the color development reagent;
w3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
A detection method of trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
q1: preparation of a solution to be tested: adding 1ml of flour or flour treating agent into a mixing container, adding 3ml of distilled water into the mixing container, stirring, and filtering by a filter head to obtain a solution to be detected;
q2: and (3) testing: and (3) taking a drop of the solution to be detected prepared in the step Q1, dropping the drop of the solution to be detected in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of trisodium trithiocyanate in the solution to be detected.
Example 3
The test paper comprises a standard colorimetric card and a color development test paper, wherein the color development test paper is prepared by infiltrating a color development agent, and the color development agent comprises saturated SnCl2The solution, hydroxypropyl methyl cellulose with the mass fraction of 2 percent, and the color development test paper is selected from rice paper.
The preparation method of the trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
s1: preparing a color developing agent: SnCl was added in portions to 100ml of distilled water2Stirring the reagent fully until SnCl2Preparing a mixed solution after the reagent is not dissolved any more, and filtering the mixed solution by using quick chromatography qualitative filter paper to obtain saturated SnCl2A solution; to saturated SnCl2Adding corresponding hydroxypropyl methyl cellulose into the solution, heating and stirring until the hydroxypropyl methyl cellulose is completely dissolved, and cooling to room temperature for later use.
S2: preparation of color test paper: soaking uncut rice paper in a color developing agent at normal temperature, soaking for 10s at room temperature, taking out, airing and cutting into a proper shape to obtain the color developing test paper.
The preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: dripping standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, wherein the color development test paper is obtained by soaking and airing the color development test paper by a color development agent;
w3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
A detection method of trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
q1: preparation of a solution to be tested: taking a 5ml medical injector, sleeving a water-based or organic filter head with the aperture not more than 0.45 mu m at the end part of a nipple of the medical injector, pulling out a piston end of the medical injector, adding 1ml of flour or flour treating agent into an empty cylinder of the medical injector, pushing the piston of the medical injector to enable the front end of the piston to be positioned at a 1ml position, then sucking 3ml of distilled water and then 1-2ml of air, violently shaking the medical injector to uniformly mix the solution in the medical injector, and pushing the piston of the injector to discard 2-3 drops of filtrate at the end part of the injector.
Q2: and (3) testing: and (3) dripping the solution in the traditional Chinese medicine injector in the step Q1 in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of the trisodium trithiocyanate in the solution to be detected.
Example 4
The test paper comprises a standard colorimetric card and a color development test paper, wherein the color development test paper is prepared by infiltrating a color development agent, and the color development agent comprises saturated NiSO4The color developing solution comprises a solution and a thickening agent with the mass fraction of 0.1%, wherein the thickening agent comprises sodium carboxymethylcellulose and hydroxypropyl methylcellulose, the mass ratio of the sodium carboxymethylcellulose to the hydroxypropyl methylcellulose is 1:1, and the color developing test paper is selected from filter paper.
The preparation method of the trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
s1: preparing a color developing agent: adding NiSO into 100ml distilled water for multiple times4Stirring the reagent fully until NiSO4Preparing a mixed solution after the reagent is not dissolved any more, and filtering the mixed solution by using quick chromatography qualitative filter paper to obtain saturated NiSO4A solution; to saturated NiSO4Adding the corresponding thickening agent into the solution, heating and stirring until the thickening agent is completely dissolved, and cooling to room temperature for later use.
S2: preparation of color test paper: and (3) soaking uncut filter paper in a color developing agent at normal temperature, taking out and airing after soaking for 10s at the room temperature, and cutting into a proper shape to obtain the color developing test paper.
The preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: dripping standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, wherein the color development test paper is obtained by soaking and airing the color development test paper by a color development agent;
w3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
A detection method of trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
q1: preparation of a solution to be tested: taking a 5ml medical injector, sleeving a water-based or organic filter head with the aperture not more than 0.45 mu m at the end part of a nipple of the medical injector, pulling out a piston end of the medical injector, adding 1ml of flour or flour treating agent into an empty cylinder of the medical injector, pushing the piston of the medical injector to enable the front end of the piston to be positioned at a 1ml position, then sucking 3ml of distilled water and then 1-2ml of air, violently shaking the medical injector to uniformly mix the solution in the medical injector, and pushing the piston of the injector to discard 2-3 drops of filtrate at the end part of the injector.
Q2: and (3) testing: and (3) dripping the solution in the traditional Chinese medicine injector in the step Q1 in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of the trisodium trithiocyanate in the solution to be detected.
Example 5
The test paper comprises a standard colorimetric card and a chromogenic test paper, wherein the chromogenic test paper is prepared by infiltrating a chromogenic agent, and the chromogenic agent comprises saturated CuSO4Solution, sodium carboxymethylcellulose with mass fraction of 1%, and developingThe test paper is selected from slow chromatography qualitative analysis filter paper.
The preparation method of the trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
s1: preparing a color developing agent: adding CuSO into 100ml distilled water for several times4Stirring the reagent fully until the reaction solution is CuSO4Preparing a mixed solution after the dissolution is not continued, and filtering the mixed solution by using quick chromatography qualitative filter paper to obtain saturated CuSO4A solution; to saturated CuSO4Adding corresponding sodium carboxymethylcellulose into the solution, heating and stirring until the sodium carboxymethylcellulose is completely dissolved, and cooling to room temperature for later use.
S2: preparation of color test paper: and (3) soaking uncut slow chromatographic qualitative analysis filter paper in a color developing agent at normal temperature, soaking at the room temperature for 10s, taking out, airing and cutting into a proper shape to obtain the color developing test paper.
The preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: dripping standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, wherein the color development test paper is obtained by soaking and airing the color development test paper by a color development agent;
w3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
A detection method of trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
q1: preparation of a solution to be tested: taking a 5ml medical injector, sleeving a water-based or organic filter head with the aperture not more than 0.45 mu m at the end part of a nipple of the medical injector, pulling out a piston end of the medical injector, adding 1ml of flour or flour treating agent into an empty cylinder of the medical injector, pushing the piston of the medical injector to enable the front end of the piston to be positioned at a 1ml position, then sucking 3ml of distilled water and then 1-2ml of air, violently shaking the medical injector to uniformly mix the solution in the medical injector, and pushing the piston of the injector to discard 2-3 drops of filtrate at the end part of the injector.
Q2: and (3) testing: and (3) dripping the solution in the traditional Chinese medicine injector in the step Q1 in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of the trisodium trithiocyanate in the solution to be detected.
Example 6
The test paper comprises a standard colorimetric card and a chromogenic test paper, wherein the chromogenic test paper is prepared by infiltrating a chromogenic agent, and the chromogenic agent comprises saturated CuSO4The solution and the chromogenic test paper are selected from slow chromatography qualitative analysis filter paper.
The preparation method of the trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
s1: preparing a color developing agent: adding CuSO into 100ml distilled water for several times4Stirring the reagent fully until the reaction solution is CuSO4Preparing a mixed solution after the dissolution is not continued, and filtering the mixed solution by using quick chromatography qualitative filter paper to obtain saturated CuSO4A solution;
s2: preparation of color test paper: and (3) soaking uncut slow chromatographic qualitative analysis filter paper in a color developing agent at normal temperature, soaking at the room temperature for 10s, taking out, airing and cutting into a proper shape to obtain the color developing test paper.
The preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: dripping standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for 30s, wherein the color development test paper is obtained by soaking and airing the color development test paper by a color development agent;
w3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
A detection method of trisodium trithiocyanate rapid detection test paper at least comprises the following steps:
q1: preparation of a solution to be tested: taking a 5ml medical injector, sleeving a water-based or organic filter head with the aperture not more than 0.45 mu m at the end part of a nipple of the medical injector, pulling out a piston end of the medical injector, adding 1ml of flour or flour treating agent into an empty cylinder of the medical injector, pushing the piston of the medical injector to enable the front end of the piston to be positioned at a 1ml position, then sucking 3ml of distilled water and then 1-2ml of air, violently shaking the medical injector to uniformly mix the solution in the medical injector, and pushing the piston of the injector to discard 2-3 drops of filtrate at the end part of the injector.
Q2: and (3) testing: and (3) dripping the solution in the traditional Chinese medicine injector in the step Q1 in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of the trisodium trithiocyanate in the solution to be detected.
Evaluation of Performance
(1) And (3) performing color spot concentration neutral analysis: the results of the detection of the color test paper prepared in example 1 and example 6 and the positive flour according to the detection method described in example 1 are shown in fig. 1 (fig. 1 is a black-and-white picture, so color cannot be displayed), the color test paper treated by the thickener in example 1 has dispersed and concentrated color spots and sensitive color development; in contrast, example 6 is a color test paper without thickener treatment, and the color spots were dispersed.
(2) And (3) analyzing the color development effect: the initial color of the color test paper prepared according to example 1 is light blue, and the trisodium trithiocyanate is detected at the mass concentrations of 0.000%, 0.001%, 0.005%, 0.01%, 0.05%, 0.1%, 1%, and 10%, respectively, as shown in fig. 1, the color test paper is not colored at the concentrations of 0.000%, 0.001%, and 0.005%, the color test paper is still light blue, and the color test paper is normally colored and gradually darkened when the concentration of trisodium trithiocyanate is greater than or equal to 0.01%, so the minimum detection concentration of the color test paper prepared in this example is 0.01%.
(3) And (3) detection capability analysis: in the detection method of the embodiment, the component to be detected is prepared by adding 3ml of distilled water into 1g of flour or flour treating agent, so that the lowest detection concentration of trisodium trithiocyanate in the flour and flour treating agent is 0.03%. The effect of adding trisodium trithiocyanate into the flour is mainly to change the flour mass curve of the flour, so that the effect of changing the gluten force is achieved, and researches show that the research results are shown in figures 2-9, when the addition amount of trisodium trithiocyanate in the flour reaches 0.05%, the maximum resistance, the tensile resistance, the stabilization time and the weakening degree of the dough are obviously changed, namely the effect of adding trisodium trithiocyanate is achieved, the addition amount of trisodium trithiocyanate in the flour is at least 0.05%, and the concentration range is in the detection range of the embodiment 1.
(4) Standard colorimetric card: the color of the standard color chart prepared according to example 1 is shown in table 1, and the color of the standard color chart is schematically shown in fig. 10.
Table 1 color of the standard color chart of example 1
| Serial number | Concentration of TMT-Na% | Colour(s) |
| 1 | 0.01 | |
| 2 | 0.05 | Light yellow |
| 3 | 0.1 | |
| 4 | 1 | Deep yellow |
| 5 | 10 | Iron oxide yellow |
As can be seen from table 1, the indicated colour changes from lemon to yellow, as the concentration of trisodium trithiocyanate goes from low to high.
(5) And (3) batch detection results: some wheat flour and flour treatment agents on the market are detected by using the color development test paper prepared in example 1, and the detection results are compared with the detection results of liquid chromatography, and the comparison results are shown in table 2.
Table 2 batch test results of example 1
(6) Analysis of detection efficiency
The method is characterized in that the wheat flour and the flour treating agent on the market are detected by adopting a liquid chromatography, and the total time consumption is more than 4 hours through the steps of on-site sampling (time consumption is 2 hours), laboratory sample pretreatment (time consumption is 0.5 hour) and instrument analysis (time consumption is 1.5 hours).
The color test paper prepared in example 1 is used for detecting wheat flour and flour treatment agents on the market, and the total time is 2min after sample pretreatment (time is 1min) and color analysis (time is 1 min). Compared with a liquid chromatography method, the method saves more than 99 percent of time.
(7) Cost analysis
The liquid chromatography is adopted to detect some wheat flour and flour treating agents in the market, and the detection cost is 600-800 yuan/batch. The test cost of the color test paper prepared in example 1 is 0.05 yuan/batch, and the test cost is about one ten-thousandth of that of liquid chromatography.
The foregoing embodiments are merely illustrative of the principles of the present invention and its efficacy, and are not to be construed as limiting the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (10)
1. The trisodium trithiocyanate rapid detection test paper is characterized by comprising the following components in parts by weight: the test paper comprises a standard colorimetric card and color development test paper, wherein the color development test paper is prepared by infiltrating a color development agent, the color development agent comprises a saturated inorganic metal salt solution, and metal ions of the saturated inorganic metal salt solution are selected from Cu2+、Fe2+、Sn2+、Ni2+Any one of the above.
2. The trisodium trithiocyanate rapid test paper according to claim 1, which is characterized in that: the developer also comprises a thickening agent with the mass fraction of 0.1-2%.
3. The trisodium trithiocyanate rapid test paper according to claim 2, wherein: the color developing agent comprises saturated CuSO4The solution and a thickening agent, wherein the thickening agent is one or a mixture of two of sodium carboxymethyl cellulose and hydroxypropyl methyl cellulose.
4. The trisodium trithiocyanate rapid test paper according to claim 1, which is characterized in that: the color test paper is selected from any one of filter paper and rice paper.
5. The trisodium trithiocyanate rapid test paper according to claim 4, wherein: the color test paper is selected from slow chromatography qualitative analysis filter paper.
6. The method for preparing trisodium trithiocyanate rapid test paper according to any one of claims 1 to 5, characterized by comprising at least the following steps:
s1: preparing a color developing agent: adding an inorganic metal salt reagent into distilled water for multiple times, fully stirring until the inorganic metal salt reagent is not dissolved any more to obtain a mixed solution, and filtering the mixed solution by using quick chromatography qualitative filter paper to obtain a saturated inorganic metal salt solution;
s2: preparation of color test paper: soaking uncut filter paper or rice paper in a color developing agent at normal temperature, soaking at room temperature for 10s, taking out, airing and cutting into a proper shape to obtain the color developing test paper.
7. The method for preparing trisodium trithiocyanate rapid test paper according to claim 6, wherein the method comprises the following steps: the preparation method of the color developing agent also comprises the following steps: and (4) adding the corresponding thickening agent into the inorganic metal salt solution prepared in the step (S1), heating and stirring until the thickening agent is completely dissolved, and cooling to room temperature for later use.
8. The method for preparing trisodium trithiocyanate rapid test paper according to claim 6, wherein the method comprises the following steps: the preparation method of the standard colorimetric card comprises the following steps:
w1: preparing 0.00%, 0.01%, 0.05%, 0.1%, 1% and 10% of standard trisodium trithiocyanate aqueous solution;
w2: dripping standard trisodium trithiocyanate solution with different concentrations in the step W1 on color development test paper for color development for more than 30s, wherein the color development test paper is obtained by soaking and airing the color development test paper by a color development agent;
w3: according to the color development effect of standard trisodium trithiocyanate solution with different concentrations on color test paper, a colorimeter is used for measuring the color value of the color development of the test paper, color module software is used for simulating color data, and a standard color comparison card is manufactured according to the standard solution concentration from low to high.
9. The detection method of trisodium trithiocyanate rapid detection test paper is characterized by at least comprising the following steps:
q1: preparation of a solution to be tested: adding 1ml of flour or flour treating agent into a mixing container, adding 3ml of distilled water into the mixing container, stirring, and filtering to obtain a solution to be detected;
q2: and (3) testing: and (3) taking a drop of the solution to be detected prepared in the step Q1, dropping the drop of the solution to be detected in the middle of the color development test paper, observing the color of the display test paper after 30s, and comparing the color with a standard colorimetric card to determine the concentration range of trisodium trithiocyanate in the solution to be detected.
10. The detection method of trisodium trithiocyanate rapid detection test paper according to claim 9, characterized in that: the mixing container described in the step Q1 is a 5ml medical syringe, the end of the nipple of the medical syringe is sleeved with a water-based or organic filter head with the aperture not more than 0.45 μm, the piston end of the medical syringe is pulled out, 1ml of flour or flour treating agent is added into an empty cylinder of the medical syringe, the piston of the medical syringe is pushed to enable the front end of the piston to be positioned at the position of 1ml, then 3ml of distilled water is sucked, 1-2ml of air is continuously sucked, the medical syringe is vigorously shaken to enable the solution in the medical syringe to be uniformly mixed, and the piston of the syringe is pushed to discard 2-3 drops of filtrate at the end of the syringe.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020011447A1 (en) * | 1999-09-30 | 2002-01-31 | Waldmann John J. | Chemical composition for treatment of nitrate and odors from water streams and process wastewater treatment |
| CN1624457A (en) * | 2003-12-05 | 2005-06-08 | 中国科学院南京土壤研究所 | Colorimetric test paper and its preparation method |
| CN101294912A (en) * | 2008-06-04 | 2008-10-29 | 山西大学 | Tea polyphenol detecting test paper, standard color comparison card and usage thereof |
| CN102207466A (en) * | 2011-04-06 | 2011-10-05 | 江苏财经职业技术学院 | Rapid test kit for benzoyl peroxide (banned additive) in flour and preparation method thereof |
| CN102374988A (en) * | 2010-08-19 | 2012-03-14 | 华东师范大学 | Trithiocyanuric acid dressed gold-size nanoprobe-based colorimetric determination method of mercury ions |
| CN103755067A (en) * | 2014-01-03 | 2014-04-30 | 上海丰信环保科技有限公司 | Treatment method for removing complex nickel out of wastewater |
| CN104237229A (en) * | 2014-08-22 | 2014-12-24 | 天津商业大学 | Ph value testing liquid and preparation method thereof |
| KR20160089218A (en) * | 2015-01-19 | 2016-07-27 | 한국과학기술연구원 | Surface modified nanoparticles, preparation method thereof, the colormetric detection sensor of copper ions (ii) and the colormetric detection method |
| CN109731693A (en) * | 2019-01-28 | 2019-05-10 | 西安建筑科技大学 | A kind of method of trithiocyanuric acid saturated solution sulfide flotation copper-lead Zinc oxide |
| CN111024687A (en) * | 2019-12-23 | 2020-04-17 | 广东环凯微生物科技有限公司 | Glutaraldehyde determination test paper and rapid determination method |
-
2020
- 2020-08-04 CN CN202010773545.1A patent/CN111812086A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020011447A1 (en) * | 1999-09-30 | 2002-01-31 | Waldmann John J. | Chemical composition for treatment of nitrate and odors from water streams and process wastewater treatment |
| CN1624457A (en) * | 2003-12-05 | 2005-06-08 | 中国科学院南京土壤研究所 | Colorimetric test paper and its preparation method |
| CN101294912A (en) * | 2008-06-04 | 2008-10-29 | 山西大学 | Tea polyphenol detecting test paper, standard color comparison card and usage thereof |
| CN102374988A (en) * | 2010-08-19 | 2012-03-14 | 华东师范大学 | Trithiocyanuric acid dressed gold-size nanoprobe-based colorimetric determination method of mercury ions |
| CN102207466A (en) * | 2011-04-06 | 2011-10-05 | 江苏财经职业技术学院 | Rapid test kit for benzoyl peroxide (banned additive) in flour and preparation method thereof |
| CN103755067A (en) * | 2014-01-03 | 2014-04-30 | 上海丰信环保科技有限公司 | Treatment method for removing complex nickel out of wastewater |
| CN104237229A (en) * | 2014-08-22 | 2014-12-24 | 天津商业大学 | Ph value testing liquid and preparation method thereof |
| KR20160089218A (en) * | 2015-01-19 | 2016-07-27 | 한국과학기술연구원 | Surface modified nanoparticles, preparation method thereof, the colormetric detection sensor of copper ions (ii) and the colormetric detection method |
| CN109731693A (en) * | 2019-01-28 | 2019-05-10 | 西安建筑科技大学 | A kind of method of trithiocyanuric acid saturated solution sulfide flotation copper-lead Zinc oxide |
| CN111024687A (en) * | 2019-12-23 | 2020-04-17 | 广东环凯微生物科技有限公司 | Glutaraldehyde determination test paper and rapid determination method |
Non-Patent Citations (7)
| Title |
|---|
| LIAO DONGMEI等: "Chemistry of copper trimercaptotriazine (TMT) compounds and removal of copper from copper-ammine species by TMT", 《APPLIED ORGANOMETALLIC CHEMISTRY》 * |
| MICHAEL J. PRUSHAN 等: "Synthesis, characterization and reactivity of a trinuclear copper(II) thiocyanurate complex: A spin-frustrated molecular propeller", 《INORGANIC CHEMISTRY COMMUNICATIONS》 * |
| 侯汉娜等: "无机实验废水中银、汞、铅、锰离子综合处理研究", 《湖北第二师范学院学报》 * |
| 刘岩峰主编: "《无机化学实验 第2版》", 28 February 2018, 哈尔滨工程大学出版社 * |
| 廖冬梅等: "三巯基均三嗪三钠盐处理脱硫废水及沉淀产物性质研究", 《热力发电》 * |
| 廖冬梅等: "用TMT处理含铜氨络合物废水的研究", 《中国给水排水》 * |
| 杨建高: "三聚硫氰酸三钠盐的应用及研究进展", 《化工中间体》 * |
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Application publication date: 20201023 |