CN111808863B - Codon-optimized coagulation factor VIII gene and construct thereof - Google Patents

Abstract

The invention relates to the field of biological medicine, in particular to a coagulation factor VIII gene optimized by codons and a construction body thereof. The invention provides a coagulation factor VIII gene with optimized codons, wherein the GC content in the coagulation factor VIII gene sequence is at least 54%, the CpG island content is at least 122, and the gene sequence of the B structural domain in the coagulation factor VIII gene with optimized codons is a truncated sequence. The invention also provides a nucleic acid construct comprising the codon-optimized factor VIII gene. The codon-optimized coagulation factor VIII gene and the construction body thereof have the following beneficial effects: the expression level of the blood coagulation factor VIII is obviously improved; the activity level of the blood coagulation factor VIII is obviously improved; the dosage is small, the immune response and the immunogenicity are reduced, and the risk is relatively controllable.

Description

Codon-optimized coagulation factor VIII gene and construct thereof

Technical Field

The invention relates to the technical field of medicines, in particular to a coagulation factor VIII gene optimized by codons and a construction body thereof.

Background

Platelet coagulation factors are various protein components involved in the process of blood coagulation. The combined action of these factors is to maintain normal coagulation physiology in a bleeding state. If one or more coagulation factors are missing, a group of hereditary hemorrhagic diseases, hemophilia, results. Among them, the most clinically common hemophilia a is caused by coagulation factor VIII-antihemophilic globulin a (ahga), which is an X-linked recessive genetic disease. Patients develop coagulation disorders of varying severity and spontaneous bleeding.

The blood coagulation factor replacement therapy is the only confirmed and effective medicament for treating the type A hemophilia at present, is expensive, and can generate inhibiting factors after long-term application. Gene therapy with AAV or lentiviral vectors as a delivery vehicle allows for sustained expression of fully functional FVIII from human cells in vivo by permanent integration of the Factor Viii (FVIII) protein encoding gene into human cells or long term implantation into non-dividing cells. Because of its potential long-term efficacy and safety, it is the most promising therapeutic approach for hemophilia a. However, FVIII protein has a complex gene structure with a total length of more than 2330 amino acids, which poses certain technical challenges for purification including in vitro expression and in vivo gene vector therapy. Most of the FVIII currently used clinically is fully functional truncated FVIII (as shown in figure 1).

Improving the gene therapy vector expression cassette of FVIII protein is a key factor in determining the efficiency of gene therapy applications. First, only optimized protein expression efficiency leads to higher protein levels, which is the greatest challenge facing most current gene therapy clinical studies for hemophilia a. Plasma functional FVIII protein levels, i.e. restoration of procoagulant function, are one of the most important evaluation indicators in gene therapy studies, both preclinical and clinical stages. Clinical data published in the current time frame for gene therapy of hemophilia show that no study has achieved 100% recovery of procoagulant function.

Secondly, the optimized protein expression efficiency means that less vector dose will be required to maintain the same protein level. Gene therapy by permanently integrating protein-encoding genes into human cells, the safety risk brought by random integration sites of encoding genes is one of the most important safety considerations in gene therapy clinical research, especially in gene therapy research using lentiviral vectors as delivery means. Current research tends to suggest that the number of random integration sites (VCN) within a single cell is positively correlated with the risk of neoplasia due to random integration. Higher expression of protein to achieve unit integration means fewer random integration sites within a single cell, leading to more controllable safety risks.

Also, the less carrier dose required to maintain the same protein level, the lower the immune response and immunogenicity brought about by the delivery vehicle itself. This is particularly evident when AAV is used as a delivery vehicle, since most AAV serotypes have some degree of preexisting immunity in the human population (e.g., AAV2) and immune responses to AAV caused by repeated dosing. Lower vector doses will result in lower immune response and immunogenicity as well as more sustained protein expression.

The biggest technical challenge in improving the expression cassette of gene therapy vectors for FVIII proteins is the codon optimization of FVIII protein genes. There are many general codon optimization principles for increasing the amount of protein expression in vivo application of gene therapy vectors, and these general codon optimization principles cannot be uniformly applied to a single gene therapy vector. Different general codon optimization principles tend to present problems of mutual incompatibility. For example, changes in the composition of CpG islands or GC content of the total coding region will necessarily affect the choice of codon usage preference. Another consideration is that different codon optimisation will, in addition to giving different protein expression levels, also give rise to different post-translational modifications of FVIII and different biological activities. Codon optimization must also target the optimized in vivo use biological activity. In addition, among the different general codon optimization principles, the prior art and the recognition suggest that too high GC content affects the transcription efficiency of the gene, and thus avoiding too high GC content is an optimization principle to be repeatedly mentioned.

Disclosure of Invention

In view of the above-mentioned drawbacks of the prior art, it is an object of the present invention to provide a codon optimized factor VIII gene and constructs thereof, which solve the problems of the prior art.

In order to achieve the above objects and other related objects, a first aspect of the present invention provides a codon-optimized factor VIII gene, wherein the GC content of the gene sequence of the factor VIII gene is at least 54%, the CpG island content of the gene sequence of the factor VIII gene is at least 122, and the gene sequence of the B domain of the codon-optimized factor VIII gene is a truncated sequence.

Preferably, the GC content in the codon-optimized sequence is 54-63%, and the CpG island content is 122-248.

A second aspect provides a nucleic acid construct comprising the codon-optimized factor VIII gene.

A third aspect provides a lentivirus virally packaged from the nucleic acid construct.

A fourth aspect provides a lentiviral vector system comprising the nucleic acid construct and a helper plasmid.

In a fifth aspect, a recombinant factor VIII is provided, which is secreted from a cell after the lentivirus infects the cell.

The sixth aspect provides a composition for preventing or treating a coagulation factor deficiency disease, wherein the effective substance of the composition contains one or more of the following substances: a codon optimized factor VIII gene; the nucleic acid construct; the lentivirus.

A seventh aspect provides a cell line that is infected with the lentivirus.

An eighth aspect provides the use of the codon optimized factor VIII gene, the nucleic acid construct, the lentivirus, the recombinant factor VIII, the composition or the cell line for the preparation of a medicament for the prevention or treatment of a factor deficiency disorder.

As described above, the present invention has the following advantageous effects:

1) the expression level of the blood coagulation factor VIII can be obviously improved while the GC content is higher;

2) the activity level of the blood coagulation factor VIII can be obviously improved while the GC content is higher;

3) the dosage is small, the immune response and the immunogenicity are reduced, and the risk is relatively controllable.

Drawings

Figure 1 shows a schematic of factor VIII, full length factor VIII on the left and truncated factor VIII on the right.

FIG. 2 shows a schematic design of a factor VIII nucleic acid construct of the present invention.

FIG. 3 shows a lentiviral backbone plasmid map of the invention.

FIG. 4 shows the expression level of coagulation factor VIII in the supernatant after infection of HepG2 liver cell line with a TTR promoter-driven lentivirus as measured by Western Blot, which was measured at 20. mu.L.

FIG. 5 shows the expression level of factor VIII in the supernatant after infection of 293T cell line with lentivirus driven by EF1a promoter as measured by Western Blot, which was measured at 20. mu.L.

Detailed Description

Unless defined otherwise below, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

The term "nucleic acid construct" refers to an artificially constructed nucleic acid segment that can be introduced into a target cell or tissue, which can be a lentiviral vector comprising a vector backbone, i.e., an empty vector, and an expression framework.

The term "vector" refers to a nucleic acid fragment or polynucleotide fragment for introducing or transferring one or more nucleic acids or one or more polynucleotides into a target cell or tissue. Typically, the vector is used to introduce the foreign DNA into another cell or tissue. The vector may comprise a bacterial resistance gene for growth in bacteria and a promoter for expression of a protein of interest in an organism. The DNA may be generated in vitro by PCR or any other suitable technique or techniques known to those skilled in the art.

The term "expression cassette" refers to a sequence having the potential to encode a protein.

Through intensive research, the inventor provides a codon-optimized coagulation factor VIII gene in a first aspect, wherein the GC content in a codon-optimized sequence is at least 54%, the CpG island content is at least 122, and the gene sequence of a B structural domain in the codon-optimized coagulation factor VIII gene is a truncated sequence.

For example, the GC content may be 55% to 60%, 61% to 65%, 66% to 70%, 71% to 75%, 76% to 80%, 81% to 85%, 86% to 90%, 91% to 95%.

In one embodiment of the invention, the content of GC in the blood coagulation factor VIII gene sequence after codon optimization is 54-63%, and the content of CpG islands is 122-248.

The principles used in codon optimisation include:

1) preferentially selecting a mammalian cell biased codon according to codon usage preferences of different species;

2) avoiding hidden cleavage sites, cleavage donor and acceptor sequences, immature tailing signals, strong mRNA secondary structures, RNA instability sequences, transcription termination signals.

Codon optimization can use existing software for codon optimization of the gene of interest such as: DNAworks, UpGene, Benchling, etc.

Further, the coagulation factor VIII gene is a mammalian gene. For example, the human blood coagulation factor VIII Gene has the Gene sequence number (Gene ID: 2157).

The GC content, also known as the G + C ratio or GC ratio, is usually expressed as a percentage. The GC content calculation formula is:

[ (total number of G + C)/(total number of a + T + C + G) ]. 100%

The following conditions are met, namely called CpG islands:

1) GC content: the GC content reaches 55 percent;

2) the occurrence of dinucleotides: the frequency of CpG dinucleotides (ratio of observed value to expected value) reaches 65%;

3) sequence length: the length is not less than 200 bp.

Wherein, the observed value is the number of CpG sites actually contained in the fragment;

the calculation method of the "expected value" is: (C G)/LS. Wherein C, G represents the number of cytosines and guanines; LS stands for fragment length (length of sequence)

Specifically, the truncated B domain has an amino acid sequence length of; 21aa, the sequence is shown in SEQ ID NO. 2.

Specifically, the nucleotide sequence of the codon optimized coagulation factor VIII gene is SEQ ID NO. 3, SEQ ID NO. 8 or SEQ ID NO. 10.

Specifically, the promoter of the codon-optimized coagulation factor VIII gene is TTR or EF1 a.

In a second aspect, the invention provides a nucleic acid construct comprising said codon-optimized factor VIII gene.

Further, the nucleic acid construct is a non-viral vector or a viral vector.

The non-viral vector mediates gene transfer by using the physicochemical properties of the non-viral vector material.

Further, the viral vector is a lentiviral vector or an adeno-associated viral vector.

The vector backbone in the lentiviral vector may be a vector backbone as described in the prior art.

Further, the vector framework is pKL-CCL, and the nucleotide sequence of the lentiviral vector framework is shown in SEQ ID NO 1.

In a third aspect, the invention provides a lentivirus virally packaged from the nucleic acid construct.

In a fourth aspect, the invention provides a lentiviral vector system comprising the nucleic acid construct and a helper plasmid.

Further, the helper plasmids encode one or more nucleotide sequences of the gag and pol proteins, as well as other necessary viral packaging component nucleotide sequences, and may include packaging and envelope plasmids.

Further, the lentiviral vector system also includes a host cell, which may be selected from a variety of applicable host cells in the art, as long as it does not limit the object of the present invention. A particular suitable cell may be a lentivirus-producing cell, for example a 293T cell.

The fifth aspect of the invention provides a recombinant blood coagulation factor VIII, wherein the recombinant blood coagulation factor VIII is obtained by secretion of cells after the slow virus infects the cells.

The sixth aspect of the present invention provides a composition for preventing or treating a coagulation factor deficiency disease, wherein the effective substance comprises one or more of the following substances: a codon optimized factor VIII gene; the nucleic acid construct; the lentivirus; recombinant factor VIII.

The composition may be a pharmaceutical composition.

The form of the composition is not particularly limited, and may be in the form of various substances such as solid, liquid, gel, semifluid, aerosol, etc.

When the composition is used for preventing or treating a coagulation factor deficiency disease, an effective dose of the composition needs to be administered to a subject. Using this method, the subject is capable of normally expressing a coagulation factor.

In a seventh aspect, the invention provides a cell line infected with the lentivirus.

The cell line can be used as a biological agent for preparing products for preventing or treating the blood coagulation factor deficiency disease.

The eighth aspect of the invention provides the use of the codon optimized factor VIII gene, the nucleic acid construct, the lentivirus, the recombinant factor VIII, the composition or the cell line in the preparation of a medicament for preventing and treating the factor deficiency disease.

The application can be the application in the development, screening and pharmacological toxicology evaluation of a blood coagulation factor deficiency drug.

The coagulation factor deficiency disease in the sixth, seventh or eighth aspects of the present invention is an inherited coagulation factor deficiency disease, such as one or more of hemophilia a, hemophilia B and hemophilia C.

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.

Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments, and is not intended to limit the scope of the present invention; in the description and claims of the present application, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise.

When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.

Example 1 coagulation factor VIII Gene expression framework design

An expression frame is designed as a wild type expression frame based on an amino acid sequence of recombinant human blood coagulation factor VIII (Elite Xyntha) for injection produced by the pharmaceutical company of Peucedanum, wherein the sequence mainly comprises regions A1, A2, B, A3, A3, C1 and C2, 887 amino acids are deleted after B domain is truncated, only 21 amino acids are contained, and the expression frame is numbered as P0001.

On the basis of the expression frame P0001, 3 other expression frames P0002, P0003 and P0004 are respectively designed and respectively optimized by codons under different conditions. The optimization principle is to preferentially select the biased codons of mammalian cells while avoiding hidden cleavage sites, cleavage donor and acceptor sequences, immature tailing signals, strong secondary mRNA structures, RNA instability sequences, transcription termination signals, and the like.

P0002 is based on satisfying these conditions, making GC content as uniform as possible, and without considering CpG content. P0003 is optimized to increase GC% as much as possible and to minimize the CpG content while satisfying the above conditions. P0004, on the basis of satisfying these conditions, totally irrespective of CpG factors, increased GC% to the highest. As shown in FIG. 2, the expression cassette is driven by different promoters. The information for each expression frame is shown in Table 1.

TABLE 1 expression framework optimization

Numbering	Optimization mode	CpG content	GC content
				P0001	Wild type	55	44％
P0002	Universal optimization	204	47％
				P0003	Kanglin optimization	122	54％
P0004	Kanglin optimization	248	63％

Example 2 construction of a factor VIII Gene-expressing Lentiviral vector

The blood coagulation factor VIII gene expression frame P0001 designed in example 1 is driven by a TTR promoter, other expression frames are driven by two promoters of TTR and EF1a respectively, and then cloned into a lentivirus framework to form a lentivirus vector, wherein the lentivirus framework is derived from a3 rd generation replication-defective self-inactivation type pseudoenveloped lentivirus framework pKL-CCL based on HIV-1, which is self-prepared by Corolin Biotechnology (Hangzhou) Limited company, the nucleotide sequence is SEQ ID NO:01, and the map is shown in figure 3. The lentivirus backbone comprises a chimeric LTR promoter, an HIV-1 packaging signal (psi), a central polypurine tract (cPPT), a Rev Response Element (RRE), a polypurine fragment (PPT), a woodchuck hepatitis B virus post-transcriptional regulatory element (WPRE), a polyadenylation signal of SV40 virus (SV40pA signal), a replication initiation site of SV40 virus (SV40ori), and a self-inactivating long terminal repeat.

The coagulation factor VIII gene expression frame P0002 (driven by TTR promoter and having the nucleotide sequence SEQ ID NO:4) designed in example 1 was synthesized by Nanjing Kingsler Biotechnology Ltd, cloned between the multiple cloning sites ClaI/SalI on the lentiviral backbone pKL-CCL by a method known in the art using homologous recombination, and the sequence information was confirmed by sequencing after cloning was completed, and named as pKL-CCL-TTR-P0002 (nucleotide sequence SEQ ID NO: 5).

The wild-type expression frame P0001 (nucleotide sequence SEQ ID NO:6) of the factor VIII gene designed in example 1 was amplified by PCR as a template by a method known in the art, and cDNA obtained by reverse transcription of mRNA from a human cell line 293T (purchased from American Type Culture Collection (ATCC) and deposited as CRL-3216) was amplified in two fragments to amplify the wild-type factor VIII gene, and cloned between the multiple cloning sites AgeI/SalI on a lentiviral vector pKL-CCL-TTR-P0002 by a method known in the art through homologous recombination, and sequence information was confirmed by sequencing after cloning was completed, and the gene was named as pKL-CCL-TTR-P0001 (nucleotide sequence SEQ ID NO: 7).

Reverse transcription system and procedure:

keeping the temperature at 65 ℃ for 5min, and rapidly cooling on ice

The procedure is as follows: 45min at 42 ℃; 15min at 70 DEG C

Wild type FVIII (B region deleted) is amplified by 2 segments, and primers are as follows:

FVIII-F1：gtccactcattcttggatccaccggtgccaccatgcaaatagagctctccacctg；(SEQ ID NO:26)

FVIII-R1：cgtttcaagactggtgggttttggctagggtgtcttgaattctgggagaagc；(SEQ ID NO:27)

FVIII-F2：gcttctcccagaattcaagacaccctagccaaaacccaccagtcttgaaacg；(SEQ ID NO:28)

FVIII-R2：tccagaggttgattgtcgacgtttaaacgcggccgctcagtagaggtcctgtgcctcgc(SEQ ID NO:29)

and (3) amplification procedure:

the coagulation factor VIII gene expression frame P0003 (nucleotide sequence SEQ ID NO:8) designed in example 1 was synthesized by Nanjing Kingsler Biotechnology Ltd, cloned between AgeI/SalI multiple cloning sites on a lentiviral vector pKL-CCL-TTR-P0002 by a ligation method well known in the art, and sequence information was confirmed by sequencing after cloning was completed, and named pKL-CCL-TTR-P0003 (nucleotide sequence SEQ ID NO: 9).

The coagulation factor VIII gene expression frame P0004 (nucleotide sequence SEQ ID NO:10) designed in example 1 was synthesized by Nanjing Kingsler Biotechnology Ltd, cloned between AgeI/SalI multiple cloning sites on a lentiviral vector pKL-CCL-TTR-P0002 by a ligation method well known in the art, and sequence information was confirmed by sequencing after cloning was completed, and named as pKL-CCL-TTR-P0004 (nucleotide sequence SEQ ID NO: 11).

The EF1a promoter (nucleotide sequence SEQ ID NO:12) is amplified by a PCR amplification method well known in the art and pKL-CCL-EF1a (nucleotide sequence SEQ ID NO:13) is used as a template, and then is cloned between the multiple cloning sites ClaI/BamHI on a lentiviral vector pKL-CCL-TTR-P0002 by a ligation method well known in the art, and after the cloning is completed, the sequence information is confirmed by sequencing and is named as pKL-CCL-EF1a-P0002 (nucleotide sequence SEQ ID NO: 14).

EF1a amplification primer:

EF1a-F：acaaaaattcaaaattttatcgataagagcatgcgtgaggctccggtgcccgtcag；(SEQ ID NO:30)

EF1a-F：catggtggcaccggtggatccaagaatgagtcacgacacctgaaatggaag；(SEQ ID NO:31)

and (3) amplification procedure:

the EF1a promoter is amplified by PCR amplification method well known in the art and pKL-CCL-EF1a as a template, and then cloned between the multiple cloning sites ClaI/BamHI on a lentivirus expression vector pKL-CCL-TTR-P0003 by a ligation method well known in the art, and after the cloning is completed, the sequence information is confirmed by sequencing and is named as pKL-CCL-EF1a-P0003 (nucleotide sequence SEQ ID NO: 15).

The EF1a promoter is amplified by PCR amplification method well known in the art and pKL-CCL-EF1a as a template, and then cloned between the multiple cloning sites ClaI/BamHI on a lentiviral vector pKL-CCL-TTR-P0004 by a ligation method well known in the art, and after the cloning is completed, the sequence information is confirmed by sequencing and is named as pKL-CCL-EF1a-P0004 (nucleotide sequence SEQ ID NO: 16).

Example 3: blood coagulation factor VIII gene expression lentivirus package

The factor VIII gene lentiviral expression vectors constructed in example 2 (pKL-CCL-TTR-P0001, pKL-CCL-TTR-P0002, pKL-CCL-TTR-P0003, pKL-CCL-TTR-P0004, pKL-CCL-EF1a-P0002, pKL-CCL-EF1a-P0003, pKL-CCL-EF1a-P0004), envelope plasmids (pKL-Kan-Vsvg with the nucleotide sequence shown in SEQ ID NO: 17) and packaging plasmids (pKL-Kan-Rev with the nucleotide sequence shown in SEQ ID NO:18, pKL-Kan-GagPol with the nucleotide sequence shown in SEQ ID NO: 19) were co-transfected into 293T cells (purchased from American Type Culture Collection (ATCC) with the accession number CRL-3216) at the same time, packaging of coagulation factor VIII gene therapy lentiviral vectors was performed in this 293T cell line. The transfection method is PEI cationic polymer mediated eukaryotic cell transient transfection, PEI cationic polymer is PEI-Max transfection reagent (purchased from Polysciences, Cat. No. 24765-1) purchased from Polysciences, and the transfection operation is carried out according to the standard operation recommended by manufacturers, and the transfection scale is 10cm2 cell culture dish.

And after 48 hours of transfection, harvesting a lentivirus vector (transfected cell culture supernatant), firstly centrifuging for 5 minutes at the room temperature of 4000rpm on a table-type bucket crane to remove cell debris, then centrifuging for 4 hours at the temperature of 4 ℃ and 10000g to obtain virus particle sediment, adding 1ml of DMEM complete culture medium into the virus particle sediment after removing the centrifugal supernatant, re-suspending the virus particles by using a microsyringe, and subpackaging the prepared virus re-suspension at-80 ℃ for later use.

Different volumes of lentiviral vectors were seeded in 96-well cell culture plates on a pre-plated human CD4+ T cell line, MT4 cell line (purchased from shanghai seoul biotechnology limited). Cell culture supernatants infected with reporter lentiviral vector EGFP (lentiviral vector packaged with pCCL-sin-EF 1. alpha. -WPRE-EGFP as described above) were used as positive controls, and initial harvest infectious titers of lentiviral vectors were calculated by quantitative PCR and flow cytometry based on GFP signals by methods well known in the art. The primer probe sequence used for quantitative PCR was:

LTR-For primer：CTGTTGTGTGACTCTGGTAACT(SEQ ID NO:20)

LTR probe：5’-AAATCTCTAGCAGTGGCGCCCG-3’(SEQ ID NO:21)

LTR-Rev primer：TTCGCTTTCAAGTCCCTGTT(SEQ ID NO:22)

HK Forward primer 5’-GCTGTCATCTCTTGTGGGCTGT-3’(SEQ ID NO:23)

HK probe 5’-CCTGTCATGCCCACACAAATCTCTCC-3’(SEQ ID NO:24)

HK Reverse primer 5’-ACTCATGGGAGCTGCTGGTTC-3’(SEQ ID NO:25)

wherein the LTR probe has 6FAM fluorescent group at the 5 'end and MGB fluorescent group at the 3' end;

the HK probe has a CY5 fluorophore at the 5 'end and a BHQ2 fluorophore at the 3' end.

Example 4: detection of protein expression efficiency and activity of blood coagulation factor VIII gene expressed lentivirus transduced cells

The crude lentivirus vectors (pKL-CCL-TTR-P0001, pKL-CCL-TTR-P0002, pKL-CCL-TTR-P0003, pKL-CCL-TTR-P0004, pKL-CCL-EF1a-P0002, pKL-CCL-EF1a-P0003 and pKL-CCL-EF1a-P0004) packaged in the above example 3 were inoculated with lentiviruses containing different promoters respectively into HepG2 cells (purchased from Nanke Baibo organism) and 293T cells pre-plated in 24-well cell culture plates, and then HepG2 cell culture supernatants from 48h to 72h and HepG2 cell culture supernatants from 24h to 72h were collected respectively, frozen at-80 ℃ for detection of protein and Western FVIII activity, while 72h cells were collected for detection of VCN:

HepG2 cells were seeded in 24-well cell culture plates at 2.50X 10 per well⁵After 2h, the cells were transfected with pKL-CCL-TTR-P0001, pKL-CCL-TTR-P0002, pKL-CCL-TTR-P0003 and pKL-CCL-TTR-P0004 lentiviral vectors by infecting HepG2 cells with a volume gradient of 25. mu.l, 50. mu.l, 100. mu.l and 200. mu.lChanging the solution after night, changing the solution again for 48h, collecting cell culture supernatant for freezing and storing at-80 ℃ for protein Western and FVIII activity detection for 72h, and collecting cells for VCN detection at the same time;

293T cells were seeded in 24-well cell culture plates at 1.00X 10 per well⁵2h after each cell, infecting 293T cells with pKL-CCL-EF1a-P0002, pKL-CCL-EF1a-P0003 and pKL-CCL-EF1a-P0004 lentiviral vectors in volume gradients of 10 mul, 20 mul, 40 mul and 80 mul, transducing for 24h, then changing the solution, collecting cell culture supernatant for cryopreservation at-80 ℃ for protein Western and FVIII activity detection, and simultaneously collecting the cells for VCN detection;

the collected cells were washed with PBS at 4200rpm for 5min, centrifuged to collect the cells, resuspended in 50. mu.l of a quick extraction solution (Quickextract)^TMDNA Extraction solvent) (purchased from Lucigen, cat. No. QE09050) and a PCR instrument running the following program of Table 2 below was used to lyse cells and extract total DNA.

TABLE 2 PCR procedure

Temperature of	Time
		65℃	15min
68℃	15min
		95℃	10min

The Copy Number of HepG2 and 293T cell-infected lentiviruses (Vector Copy Number, VCN) was calculated from quantitative PCR data by methods well known in the art and the results are shown in tables 3 and 4.

TABLE 3 VCN after lentiviral transduction of HepG2 cells

TABLE 4 VCN after lentivirus transduction of 293T cells

Selecting a sample close to VCN for activity detection according to the detection result of the VCN, wherein the detection kit is Biophen FVIII, C (Chromogenic assay for measuring Factor VIII, C in plasma, or in concentrations), the product number is 221402, the standard product is recombinant human coagulation Factor VIII (Rijie Xyntha) for injection produced by the pharmaceutical company of Peucedanum, 500 IU/bottle, and the registration number is S20120059; the normal human plasma coagulation factor VIII activity was defined as 1IU/mL, and comparative analysis was performed based on this, and the data are shown in Table 5.

TABLE 5 detection of coagulation factor VIII Activity

The data show that, based on comparison of 24h expression activity equivalents of 1.00E +06 cells within a unit VCN, comprehensive comparison of a series of lentiviruses of the TTR promoter after infection of HepG2 cells shows an order of superiority: pKL-TTR-P0004> pKL-TTR-P0003> pKL-TTR-P0002> pKL-TTR-P0001, overall comparison pKL-TTR-P0004 being optimal, wild type pKL-TTR-P0001 being essentially undetectable; after 293T cells are infected by series of lentiviruses of the EF1a promoter, the comprehensive comparison shows that the advantages are ranked: pKL-EF1a-P0004 is similar to pKL-EF1a-P0003 and is superior to pKL-EF1 a-P0002. The codon optimization strategy of kanglin obviously improves the activity level of the blood coagulation factor VIII in the cell culture supernatant.

A part of cell culture supernatant samples were selected according to VCN results and activity detection results and subjected to Western Blot, the loading amount was 20. mu.l, the antibody was Factor VIII (R8B12) (sc-73597), the antibody was purchased from Santa Cruze, the epitope binding site was A2 domain peptides 497-.

The detection result shows that the codon optimization strategy of conlin obviously improves the expression quantity of the blood coagulation factor VIII in the cell culture supernatant under the condition of similar VCN.

The above examples are intended to illustrate the disclosed embodiments of the invention and are not to be construed as limiting the invention. In addition, various modifications of the invention set forth herein, as well as variations of the methods of the invention, will be apparent to persons skilled in the art without departing from the scope and spirit of the invention. While the invention has been specifically described in connection with various specific preferred embodiments thereof, it should be understood that the invention should not be unduly limited to such specific embodiments. Indeed, various modifications of the above-described embodiments which are obvious to those skilled in the art to which the invention pertains are intended to be covered by the scope of the present invention.

Sequence listing

<110 kang Lin Biotech (Hangzhou) Ltd

<120> a codon optimized coagulation factor VIII gene and its construct

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<170> SIPOSequenceListing 1.0

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aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga 3000

aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa 3060

gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg 3120

agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa 3180

aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa 3240

gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta 3300

gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat 3360

tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca 3420

aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag 3480

cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta 3540

tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga 3600

agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg 3660

ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa 3720

ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag 3780

catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg 3840

gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt 3900

tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg 3960

aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac 4020

tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta 4080

gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa 4140

ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct 4200

atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc 4260

ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac 4320

agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg 4380

gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa 4440

actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatga gtaattcata 4500

caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 4560

tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 4620

gtggagaaga gcatgcgtga gggatccgtc gacaatcaac ctctggatta caaaatttgt 4680

gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 4740

ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 4800

aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 4860

gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 4920

ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 4980

tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5040

tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 5100

gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5160

ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 5220

tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt 5280

acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 5340

ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 5400

agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 5460

gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 5520

gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 5580

ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5640

ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5700

catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5760

tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 5820

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 5880

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta 5940

cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 6000

cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 6060

gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 6120

ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 6180

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 6240

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 6300

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 6360

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 6420

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 6480

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 6540

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcc 6594

<210> 2

<211> 21

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 2

Ser Phe Ser Gln Asn Ser Arg His Pro Ser Gln Asn Pro Pro Val Leu

1 5 10 15

Lys Arg His Gln Arg

20

<210> 3

<211> 4395

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

atgcagatcg aactgagtac ctgcttcttt ctttgcttgt tgcgcttttg cttttctgcg 60

acgcggcggt attacttggg ggcggttgag ttgagctggg attatatgca atctgacctg 120

ggtgaactgc cggtcgatgc gcggttcccg ccccgggttc ccaaaagttt tccattcaac 180

acatccgtcg tttacaaaaa aacgcttttc gtagaattca cagatcacct gtttaacata 240

gcgaagccac gacctccctg gatgggcctt ttgggaccta cgatacaagc tgaagtatac 300

gataccgttg ttatcacact gaagaacatg gcaagtcacc ctgtttccct tcatgccgtg 360

ggagtatcat attggaaggc ttctgaagga gcagaatatg atgatcaaac aagtcaaaga 420

gagaaggaag atgacaaagt gttccctggg gggagtcata cgtacgtgtg gcaagtattg 480

aaggaaaatg gtccgatggc gtctgacccg ctttgtctta cctattccta cctttctcac 540

gtggacctgg taaaagatct gaactcaggt ctcattggcg ccctgttggt ttgtcgcgag 600

ggttcattgg caaaagaaaa gactcaaacg cttcacaagt ttatccttct ctttgccgtc 660

ttcgatgaag ggaagtcttg gcatagtgag actaagaact ccctcatgca agacagggat 720

gctgcatccg cgcgagcgtg gcctaagatg cacacggtta acggctatgt gaacaggagc 780

ctgccagggc tcatcggttg ccacaggaag tccgtgtact ggcatgttat agggatgggg 840

actacacctg aagtccattc tatattcctc gaaggacaca cctttcttgt acgaaatcac 900

cgccaagcgt ctcttgaaat ttcccctatt accttcctca ctgcacaaac ccttctgatg 960

gacctgggcc aatttcttct gttctgtcac attagttcac atcaacatga cggtatggag 1020

gcttacgtca aggtggacag ctgcccagag gaaccccaat tgcgcatgaa aaacaatgag 1080

gaagctgaag actacgacga tgatcttacg gactccgaga tggacgtggt tcgctttgat 1140

gatgataatt ctccttcttt catccaaatc cgatctgtgg caaaaaaaca tcccaagacg 1200

tgggtgcatt atatcgctgc ggaggaagaa gattgggact atgctccttt ggtgcttgca 1260

cctgatgacc gcagttataa gtcacagtac ttgaataacg gccctcaaag aatcggaaga 1320

aaatataaga aggtccgatt catggcctac accgacgaga cgttcaaaac ccgagaagct 1380

attcagcacg aaagcggaat actggggccg ctgttgtatg gtgaagtcgg agatacactt 1440

ctcataatat ttaaaaacca ggcttcacgg ccatacaaca tctatccgca tggtatcacc 1500

gacgtgcggc ccctgtatag tcggagactg cctaaggggg taaaacatct caaggatttt 1560

ccgattctcc ccggagaaat tttcaagtat aaatggacgg tgacggtcga ggatggtcct 1620

accaaatccg atccccggtg tctcacaaga tactacagca gcttcgttaa tatggaaaga 1680

gacctcgctt ccggacttat cggaccgttg ctcatatgtt acaaagagtc cgtagatcaa 1740

aggggcaacc aaattatgtc cgataagcgg aatgttatat tgttcagtgt cttcgacgag 1800

aacaggtctt ggtatttgac tgaaaacatc cagcgatttc tgccgaaccc cgcaggggta 1860

caattggagg acccggaatt ccaagctagt aatatcatgc attctatcaa tggatacgta 1920

tttgattccc ttcagcttag cgtttgtctg catgaagtcg catattggta tatccttagt 1980

attggtgctc aaactgactt cctgtctgta tttttttctg gttacacctt caagcacaag 2040

atggtctacg aggacactct tacgcttttt cccttctctg gagagacggt gtttatgagc 2100

atggaaaacc ccgggctttg gattctcggg tgccataatt cagacttccg caacaggggt 2160

atgacagcct tgttgaaggt cagctcctgc gataaaaaca ccggggatta ctatgaagac 2220

tcctacgaag acatttctgc atatctcctc tccaaaaaca acgcgatcga accaaggtct 2280

ttttcccaga actcacggca tccaagccag aatccaccag tgttgaaacg ccatcagagg 2340

gaaattacgc gaacgacctt gcaaagcgat caggaagaaa ttgattatga cgacactata 2400

agtgtagaaa tgaaaaaaga ggactttgac atctatgatg aggatgagaa ccagtcccca 2460

agaagttttc agaagaagac ccgccactat tttattgctg ccgtcgaacg gttgtgggac 2520

tacggaatga gctcctcccc gcatgtgttg cggaatcgag cccaaagtgg ctctgtgcct 2580

cagttcaaaa aggtcgtatt ccaagaattc actgatggca gcttcactca gccactgtat 2640

cggggggagt tgaacgaaca tctcggcctc ttgggcccat acatacgcgc tgaggttgaa 2700

gataacataa tggtaacttt tcgaaatcag gcatcaaggc cttattcatt ttacagctct 2760

ctcatatctt acgaagagga ccaaagacaa ggagcggaac ctcgcaagaa ttttgtaaaa 2820

cccaatgaaa cgaaaacgta tttctggaag gttcagcacc acatggcccc aacaaaggat 2880

gaatttgatt gtaaagcgtg ggcgtatttt agtgacgtcg atctcgaaaa ggatgttcat 2940

tcagggctta tcggtcccct ccttgtgtgt catacaaaca cacttaatcc ggcgcacggt 3000

agacaagtaa ctgtgcagga atttgcgttg tttttcacga tctttgatga aactaagtca 3060

tggtatttta cggagaacat ggagcggaat tgtagggcac catgtaatat acagatggaa 3120

gacccaacct ttaaagaaaa ttacagattc catgccataa acgggtacat catggatact 3180

ctcccaggac tggtaatggc tcaggaccag cgaatacgat ggtacttgct tagcatgggg 3240

agtaacgaaa acatccattc tattcatttt tcaggccatg tgttcactgt cagaaaaaag 3300

gaggagtata agatggcgct ctacaatctg taccccggtg tgtttgagac ggtagaaatg 3360

ctgccctcca aagctggtat atggagagta gagtgtttga taggagaaca tctccacgcc 3420

ggcatgtcta cgctgtttct cgtttacagc aataagtgcc agacccccct ggggatggct 3480

agtgggcaca tccgcgattt tcaaattaca gcatctgggc aatacggtca atgggcgccg 3540

aaactggcta gactgcatta ttccgggtcc attaatgctt ggtccactaa ggagcccttc 3600

agctggatca aggtagacct tctcgcgcct atgattatac acggtataaa gacccaaggt 3660

gccagacaga agtttagtag cctttacata tcacagttta ttataatgta ctccttggat 3720

ggcaagaagt ggcaaaccta taggggtaac tccacgggaa ccctgatggt ctttttcggg 3780

aacgtagact cctcaggaat aaagcacaat attttcaatc ccccaatcat agcgcgctat 3840

atacgacttc atcctacgca ttactccata cgctctacgc tgcgaatgga gctgatgggc 3900

tgcgatctga acagttgctc catgcctctg ggcatggaat ctaaagccat cagcgatgca 3960

caaattaccg ctagtagcta cttcaccaat atgtttgcca catggtcccc gtctaaggct 4020

cgcctgcatc tgcaaggccg gtccaacgca tggcgacctc aggtcaataa cccaaaggaa 4080

tggttgcagg tagactttca gaagaccatg aaggttaccg gggtaactac tcagggggta 4140

aaatcactgt tgactagcat gtacgtgaaa gaattcctca ttagcagtag tcaagatggc 4200

catcagtgga cgctgttctt tcaaaacggg aaggtcaaag ttttccaggg gaatcaggac 4260

tccttcacac ccgtcgtcaa ctcactcgat ccaccactgt tgacccggta cctgagaatc 4320

cacccacaat cctgggttca ccaaatcgca ctcaggatgg aagtactcgg gtgcgaagcg 4380

caggacctct actga 4395

<210> 4

<211> 4395

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

atgcagatcg aactgagtac ctgcttcttt ctttgcttgt tgcgcttttg cttttctgcg 60

acgcggcggt attacttggg ggcggttgag ttgagctggg attatatgca atctgacctg 120

ggtgaactgc cggtcgatgc gcggttcccg ccccgggttc ccaaaagttt tccattcaac 180

acatccgtcg tttacaaaaa aacgcttttc gtagaattca cagatcacct gtttaacata 240

gcgaagccac gacctccctg gatgggcctt ttgggaccta cgatacaagc tgaagtatac 300

gataccgttg ttatcacact gaagaacatg gcaagtcacc ctgtttccct tcatgccgtg 360

ggagtatcat attggaaggc ttctgaagga gcagaatatg atgatcaaac aagtcaaaga 420

gagaaggaag atgacaaagt gttccctggg gggagtcata cgtacgtgtg gcaagtattg 480

aaggaaaatg gtccgatggc gtctgacccg ctttgtctta cctattccta cctttctcac 540

gtggacctgg taaaagatct gaactcaggt ctcattggcg ccctgttggt ttgtcgcgag 600

ggttcattgg caaaagaaaa gactcaaacg cttcacaagt ttatccttct ctttgccgtc 660

ttcgatgaag ggaagtcttg gcatagtgag actaagaact ccctcatgca agacagggat 720

gctgcatccg cgcgagcgtg gcctaagatg cacacggtta acggctatgt gaacaggagc 780

ctgccagggc tcatcggttg ccacaggaag tccgtgtact ggcatgttat agggatgggg 840

actacacctg aagtccattc tatattcctc gaaggacaca cctttcttgt acgaaatcac 900

cgccaagcgt ctcttgaaat ttcccctatt accttcctca ctgcacaaac ccttctgatg 960

gacctgggcc aatttcttct gttctgtcac attagttcac atcaacatga cggtatggag 1020

gcttacgtca aggtggacag ctgcccagag gaaccccaat tgcgcatgaa aaacaatgag 1080

gaagctgaag actacgacga tgatcttacg gactccgaga tggacgtggt tcgctttgat 1140

gatgataatt ctccttcttt catccaaatc cgatctgtgg caaaaaaaca tcccaagacg 1200

tgggtgcatt atatcgctgc ggaggaagaa gattgggact atgctccttt ggtgcttgca 1260

cctgatgacc gcagttataa gtcacagtac ttgaataacg gccctcaaag aatcggaaga 1320

aaatataaga aggtccgatt catggcctac accgacgaga cgttcaaaac ccgagaagct 1380

attcagcacg aaagcggaat actggggccg ctgttgtatg gtgaagtcgg agatacactt 1440

ctcataatat ttaaaaacca ggcttcacgg ccatacaaca tctatccgca tggtatcacc 1500

gacgtgcggc ccctgtatag tcggagactg cctaaggggg taaaacatct caaggatttt 1560

ccgattctcc ccggagaaat tttcaagtat aaatggacgg tgacggtcga ggatggtcct 1620

accaaatccg atccccggtg tctcacaaga tactacagca gcttcgttaa tatggaaaga 1680

gacctcgctt ccggacttat cggaccgttg ctcatatgtt acaaagagtc cgtagatcaa 1740

aggggcaacc aaattatgtc cgataagcgg aatgttatat tgttcagtgt cttcgacgag 1800

aacaggtctt ggtatttgac tgaaaacatc cagcgatttc tgccgaaccc cgcaggggta 1860

caattggagg acccggaatt ccaagctagt aatatcatgc attctatcaa tggatacgta 1920

tttgattccc ttcagcttag cgtttgtctg catgaagtcg catattggta tatccttagt 1980

attggtgctc aaactgactt cctgtctgta tttttttctg gttacacctt caagcacaag 2040

atggtctacg aggacactct tacgcttttt cccttctctg gagagacggt gtttatgagc 2100

atggaaaacc ccgggctttg gattctcggg tgccataatt cagacttccg caacaggggt 2160

atgacagcct tgttgaaggt cagctcctgc gataaaaaca ccggggatta ctatgaagac 2220

tcctacgaag acatttctgc atatctcctc tccaaaaaca acgcgatcga accaaggtct 2280

ttttcccaga actcacggca tccaagccag aatccaccag tgttgaaacg ccatcagagg 2340

gaaattacgc gaacgacctt gcaaagcgat caggaagaaa ttgattatga cgacactata 2400

agtgtagaaa tgaaaaaaga ggactttgac atctatgatg aggatgagaa ccagtcccca 2460

agaagttttc agaagaagac ccgccactat tttattgctg ccgtcgaacg gttgtgggac 2520

tacggaatga gctcctcccc gcatgtgttg cggaatcgag cccaaagtgg ctctgtgcct 2580

cagttcaaaa aggtcgtatt ccaagaattc actgatggca gcttcactca gccactgtat 2640

cggggggagt tgaacgaaca tctcggcctc ttgggcccat acatacgcgc tgaggttgaa 2700

gataacataa tggtaacttt tcgaaatcag gcatcaaggc cttattcatt ttacagctct 2760

ctcatatctt acgaagagga ccaaagacaa ggagcggaac ctcgcaagaa ttttgtaaaa 2820

cccaatgaaa cgaaaacgta tttctggaag gttcagcacc acatggcccc aacaaaggat 2880

gaatttgatt gtaaagcgtg ggcgtatttt agtgacgtcg atctcgaaaa ggatgttcat 2940

tcagggctta tcggtcccct ccttgtgtgt catacaaaca cacttaatcc ggcgcacggt 3000

agacaagtaa ctgtgcagga atttgcgttg tttttcacga tctttgatga aactaagtca 3060

tggtatttta cggagaacat ggagcggaat tgtagggcac catgtaatat acagatggaa 3120

gacccaacct ttaaagaaaa ttacagattc catgccataa acgggtacat catggatact 3180

ctcccaggac tggtaatggc tcaggaccag cgaatacgat ggtacttgct tagcatgggg 3240

agtaacgaaa acatccattc tattcatttt tcaggccatg tgttcactgt cagaaaaaag 3300

gaggagtata agatggcgct ctacaatctg taccccggtg tgtttgagac ggtagaaatg 3360

ctgccctcca aagctggtat atggagagta gagtgtttga taggagaaca tctccacgcc 3420

ggcatgtcta cgctgtttct cgtttacagc aataagtgcc agacccccct ggggatggct 3480

agtgggcaca tccgcgattt tcaaattaca gcatctgggc aatacggtca atgggcgccg 3540

aaactggcta gactgcatta ttccgggtcc attaatgctt ggtccactaa ggagcccttc 3600

agctggatca aggtagacct tctcgcgcct atgattatac acggtataaa gacccaaggt 3660

gccagacaga agtttagtag cctttacata tcacagttta ttataatgta ctccttggat 3720

ggcaagaagt ggcaaaccta taggggtaac tccacgggaa ccctgatggt ctttttcggg 3780

aacgtagact cctcaggaat aaagcacaat attttcaatc ccccaatcat agcgcgctat 3840

atacgacttc atcctacgca ttactccata cgctctacgc tgcgaatgga gctgatgggc 3900

tgcgatctga acagttgctc catgcctctg ggcatggaat ctaaagccat cagcgatgca 3960

caaattaccg ctagtagcta cttcaccaat atgtttgcca catggtcccc gtctaaggct 4020

cgcctgcatc tgcaaggccg gtccaacgca tggcgacctc aggtcaataa cccaaaggaa 4080

tggttgcagg tagactttca gaagaccatg aaggttaccg gggtaactac tcagggggta 4140

aaatcactgt tgactagcat gtacgtgaaa gaattcctca ttagcagtag tcaagatggc 4200

catcagtgga cgctgttctt tcaaaacggg aaggtcaaag ttttccaggg gaatcaggac 4260

tccttcacac ccgtcgtcaa ctcactcgat ccaccactgt tgacccggta cctgagaatc 4320

cacccacaat cctgggttca ccaaatcgca ctcaggatgg aagtactcgg gtgcgaagcg 4380

caggacctct actga 4395

<210> 5

<211> 11341

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 5

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga 3000

aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 3060

gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 3120

gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 3180

ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 3240

gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 3300

aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 3360

ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 3420

aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 3480

ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 3540

aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 3600

gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 3660

gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta 3720

ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 3780

ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 3840

agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 3900

accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 3960

cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 4020

ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 4080

aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 4140

ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 4200

gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 4260

aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga 4320

tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg 4380

attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 4440

aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatggggg aggctgctgg 4500

tgaatattaa ccaaggtcac cccagttatc ggaggagcaa acaggggcta agtccactct 4560

tgcatctaaa atgagagaca aaaaatctat aaaaatggaa aacatgcata gaaatatgtg 4620

agggaggaaa aaattacccc caagaatgtt agtgcacgca gtcacacagg gagaagacta 4680

tttttgtttt gttttgattg ttttgttttg ttttggttgt tttgttttgg tgacctaact 4740

ggtcaaatga cctattaaga atatttcata gaacgaatgt tccgatgctc taatctctct 4800

agacaaggtt catatttgta tgggttactt attctctctt tgttgactaa gtcaataatc 4860

agaatcagca ggtttgcagt cagattggca gggataagca gcctagctca ggagaagtga 4920

gtataaaagc cccaggctgg gagcagccat cacagaagtc cactcattct tggatccacc 4980

ggtgccacca tgcagatcga actgagtacc tgcttctttc tttgcttgtt gcgcttttgc 5040

ttttctgcga cgcggcggta ttacttgggg gcggttgagt tgagctggga ttatatgcaa 5100

tctgacctgg gtgaactgcc ggtcgatgcg cggttcccgc cccgggttcc caaaagtttt 5160

ccattcaaca catccgtcgt ttacaaaaaa acgcttttcg tagaattcac agatcacctg 5220

tttaacatag cgaagccacg acctccctgg atgggccttt tgggacctac gatacaagct 5280

gaagtatacg ataccgttgt tatcacactg aagaacatgg caagtcaccc tgtttccctt 5340

catgccgtgg gagtatcata ttggaaggct tctgaaggag cagaatatga tgatcaaaca 5400

agtcaaagag agaaggaaga tgacaaagtg ttccctgggg ggagtcatac gtacgtgtgg 5460

caagtattga aggaaaatgg tccgatggcg tctgacccgc tttgtcttac ctattcctac 5520

ctttctcacg tggacctggt aaaagatctg aactcaggtc tcattggcgc cctgttggtt 5580

tgtcgcgagg gttcattggc aaaagaaaag actcaaacgc ttcacaagtt tatccttctc 5640

tttgccgtct tcgatgaagg gaagtcttgg catagtgaga ctaagaactc cctcatgcaa 5700

gacagggatg ctgcatccgc gcgagcgtgg cctaagatgc acacggttaa cggctatgtg 5760

aacaggagcc tgccagggct catcggttgc cacaggaagt ccgtgtactg gcatgttata 5820

gggatgggga ctacacctga agtccattct atattcctcg aaggacacac ctttcttgta 5880

cgaaatcacc gccaagcgtc tcttgaaatt tcccctatta ccttcctcac tgcacaaacc 5940

cttctgatgg acctgggcca atttcttctg ttctgtcaca ttagttcaca tcaacatgac 6000

ggtatggagg cttacgtcaa ggtggacagc tgcccagagg aaccccaatt gcgcatgaaa 6060

aacaatgagg aagctgaaga ctacgacgat gatcttacgg actccgagat ggacgtggtt 6120

cgctttgatg atgataattc tccttctttc atccaaatcc gatctgtggc aaaaaaacat 6180

cccaagacgt gggtgcatta tatcgctgcg gaggaagaag attgggacta tgctcctttg 6240

gtgcttgcac ctgatgaccg cagttataag tcacagtact tgaataacgg ccctcaaaga 6300

atcggaagaa aatataagaa ggtccgattc atggcctaca ccgacgagac gttcaaaacc 6360

cgagaagcta ttcagcacga aagcggaata ctggggccgc tgttgtatgg tgaagtcgga 6420

gatacacttc tcataatatt taaaaaccag gcttcacggc catacaacat ctatccgcat 6480

ggtatcaccg acgtgcggcc cctgtatagt cggagactgc ctaagggggt aaaacatctc 6540

aaggattttc cgattctccc cggagaaatt ttcaagtata aatggacggt gacggtcgag 6600

gatggtccta ccaaatccga tccccggtgt ctcacaagat actacagcag cttcgttaat 6660

atggaaagag acctcgcttc cggacttatc ggaccgttgc tcatatgtta caaagagtcc 6720

gtagatcaaa ggggcaacca aattatgtcc gataagcgga atgttatatt gttcagtgtc 6780

ttcgacgaga acaggtcttg gtatttgact gaaaacatcc agcgatttct gccgaacccc 6840

gcaggggtac aattggagga cccggaattc caagctagta atatcatgca ttctatcaat 6900

ggatacgtat ttgattccct tcagcttagc gtttgtctgc atgaagtcgc atattggtat 6960

atccttagta ttggtgctca aactgacttc ctgtctgtat ttttttctgg ttacaccttc 7020

aagcacaaga tggtctacga ggacactctt acgctttttc ccttctctgg agagacggtg 7080

tttatgagca tggaaaaccc cgggctttgg attctcgggt gccataattc agacttccgc 7140

aacaggggta tgacagcctt gttgaaggtc agctcctgcg ataaaaacac cggggattac 7200

tatgaagact cctacgaaga catttctgca tatctcctct ccaaaaacaa cgcgatcgaa 7260

ccaaggtctt tttcccagaa ctcacggcat ccaagccaga atccaccagt gttgaaacgc 7320

catcagaggg aaattacgcg aacgaccttg caaagcgatc aggaagaaat tgattatgac 7380

gacactataa gtgtagaaat gaaaaaagag gactttgaca tctatgatga ggatgagaac 7440

cagtccccaa gaagttttca gaagaagacc cgccactatt ttattgctgc cgtcgaacgg 7500

ttgtgggact acggaatgag ctcctccccg catgtgttgc ggaatcgagc ccaaagtggc 7560

tctgtgcctc agttcaaaaa ggtcgtattc caagaattca ctgatggcag cttcactcag 7620

ccactgtatc ggggggagtt gaacgaacat ctcggcctct tgggcccata catacgcgct 7680

gaggttgaag ataacataat ggtaactttt cgaaatcagg catcaaggcc ttattcattt 7740

tacagctctc tcatatctta cgaagaggac caaagacaag gagcggaacc tcgcaagaat 7800

tttgtaaaac ccaatgaaac gaaaacgtat ttctggaagg ttcagcacca catggcccca 7860

acaaaggatg aatttgattg taaagcgtgg gcgtatttta gtgacgtcga tctcgaaaag 7920

gatgttcatt cagggcttat cggtcccctc cttgtgtgtc atacaaacac acttaatccg 7980

gcgcacggta gacaagtaac tgtgcaggaa tttgcgttgt ttttcacgat ctttgatgaa 8040

actaagtcat ggtattttac ggagaacatg gagcggaatt gtagggcacc atgtaatata 8100

cagatggaag acccaacctt taaagaaaat tacagattcc atgccataaa cgggtacatc 8160

atggatactc tcccaggact ggtaatggct caggaccagc gaatacgatg gtacttgctt 8220

agcatgggga gtaacgaaaa catccattct attcattttt caggccatgt gttcactgtc 8280

agaaaaaagg aggagtataa gatggcgctc tacaatctgt accccggtgt gtttgagacg 8340

gtagaaatgc tgccctccaa agctggtata tggagagtag agtgtttgat aggagaacat 8400

ctccacgccg gcatgtctac gctgtttctc gtttacagca ataagtgcca gacccccctg 8460

gggatggcta gtgggcacat ccgcgatttt caaattacag catctgggca atacggtcaa 8520

tgggcgccga aactggctag actgcattat tccgggtcca ttaatgcttg gtccactaag 8580

gagcccttca gctggatcaa ggtagacctt ctcgcgccta tgattataca cggtataaag 8640

acccaaggtg ccagacagaa gtttagtagc ctttacatat cacagtttat tataatgtac 8700

tccttggatg gcaagaagtg gcaaacctat aggggtaact ccacgggaac cctgatggtc 8760

tttttcggga acgtagactc ctcaggaata aagcacaata ttttcaatcc cccaatcata 8820

gcgcgctata tacgacttca tcctacgcat tactccatac gctctacgct gcgaatggag 8880

ctgatgggct gcgatctgaa cagttgctcc atgcctctgg gcatggaatc taaagccatc 8940

agcgatgcac aaattaccgc tagtagctac ttcaccaata tgtttgccac atggtccccg 9000

tctaaggctc gcctgcatct gcaaggccgg tccaacgcat ggcgacctca ggtcaataac 9060

ccaaaggaat ggttgcaggt agactttcag aagaccatga aggttaccgg ggtaactact 9120

cagggggtaa aatcactgtt gactagcatg tacgtgaaag aattcctcat tagcagtagt 9180

caagatggcc atcagtggac gctgttcttt caaaacggga aggtcaaagt tttccagggg 9240

aatcaggact ccttcacacc cgtcgtcaac tcactcgatc caccactgtt gacccggtac 9300

ctgagaatcc acccacaatc ctgggttcac caaatcgcac tcaggatgga agtactcggg 9360

tgcgaagcgc aggacctcta ctgagcggcc gcgtttaaac gtcgacaatc aacctctgga 9420

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 9480

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 9540

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 9600

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 9660

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 9720

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 9780

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 9840

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 9900

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 9960

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 10020

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10080

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10140

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 10200

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10260

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 10320

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 10380

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10440

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10500

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 10560

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10620

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 10680

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 10740

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 10800

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 10860

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 10920

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 10980

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 11040

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11100

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11160

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11220

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 11280

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 11340

c 11341

<210> 6

<211> 4395

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 6

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctgggg tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagccaa aacccaccag tcttgaaacg ccatcaacgg 2340

gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400

tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460

cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520

tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580

cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640

cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700

gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760

cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820

cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880

gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940

tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000

agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060

tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120

gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180

ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240

agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300

gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360

ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420

gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480

tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540

aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600

tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660

gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720

gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780

aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840

atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900

tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960

cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020

cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080

tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140

aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200

catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260

tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320

cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380

caggacctct actga 4395

<210> 7

<211> 11341

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga 3000

aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 3060

gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 3120

gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 3180

ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 3240

gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 3300

aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 3360

ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 3420

aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 3480

ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 3540

aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 3600

gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 3660

gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta 3720

ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 3780

ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 3840

agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 3900

accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 3960

cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 4020

ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 4080

aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 4140

ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 4200

gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 4260

aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga 4320

tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg 4380

attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 4440

aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatggggg aggctgctgg 4500

tgaatattaa ccaaggtcac cccagttatc ggaggagcaa acaggggcta agtccactct 4560

tgcatctaaa atgagagaca aaaaatctat aaaaatggaa aacatgcata gaaatatgtg 4620

agggaggaaa aaattacccc caagaatgtt agtgcacgca gtcacacagg gagaagacta 4680

tttttgtttt gttttgattg ttttgttttg ttttggttgt tttgttttgg tgacctaact 4740

ggtcaaatga cctattaaga atatttcata gaacgaatgt tccgatgctc taatctctct 4800

agacaaggtt catatttgta tgggttactt attctctctt tgttgactaa gtcaataatc 4860

agaatcagca ggtttgcagt cagattggca gggataagca gcctagctca ggagaagtga 4920

gtataaaagc cccaggctgg gagcagccat cacagaagtc cactcattct tggatccacc 4980

ggtgccacca tgcaaataga gctctccacc tgcttctttc tgtgcctttt gcgattctgc 5040

tttagtgcca ccagaagata ctacctgggt gcagtggaac tgtcatggga ctatatgcaa 5100

agtgatctcg gtgagctgcc tgtggacgca agatttcctc ctagagtgcc aaaatctttt 5160

ccattcaaca cctcagtcgt gtacaaaaag actctgtttg tagaattcac ggatcacctt 5220

ttcaacatcg ctaagccaag gccaccctgg atgggtctgc taggtcctac catccaggct 5280

gaggtttatg atacagtggt cattacactt aagaacatgg cttcccatcc tgtcagtctt 5340

catgctgttg gtgtatccta ctggaaagct tctgagggag ctgaatatga tgatcagacc 5400

agtcaaaggg agaaagaaga tgataaagtc ttccctggtg gaagccatac atatgtctgg 5460

caggtcctga aagagaatgg tccaatggcc tctgacccac tgtgccttac ctactcatat 5520

ctttctcatg tggacctggt aaaagacttg aattcaggcc tcattggagc cctactagta 5580

tgtagagaag ggagtctggc caaggaaaag acacagacct tgcacaaatt tatactactt 5640

tttgctgtat ttgatgaagg gaaaagttgg cactcagaaa caaagaactc cttgatgcag 5700

gatagggatg ctgcatctgc tcgggcctgg cctaaaatgc acacagtcaa tggttatgta 5760

aacaggtctc tgccaggtct gattggatgc cacaggaaat cagtctattg gcatgtgatt 5820

ggaatgggca ccactcctga agtgcactca atattcctcg aaggtcacac atttcttgtg 5880

aggaaccatc gccaggcgtc cttggaaatc tcgccaataa ctttccttac tgctcaaaca 5940

ctcttgatgg accttggaca gtttctactg ttttgtcata tctcttccca ccaacatgat 6000

ggcatggaag cttatgtcaa agtagacagc tgtccagagg aaccccaact acgaatgaaa 6060

aataatgaag aagcggaaga ctatgatgat gatcttactg attctgaaat ggatgtggtc 6120

aggtttgatg atgacaactc tccttccttt atccaaattc gctcagttgc caagaagcat 6180

cctaaaactt gggtacatta cattgctgct gaagaggagg actgggacta tgctccctta 6240

gtcctcgccc ccgatgacag aagttataaa agtcaatatt tgaacaatgg ccctcagcgg 6300

attggtagga agtacaaaaa agtccgattt atggcataca cagatgaaac ctttaagact 6360

cgtgaagcta ttcagcatga atcaggaatc ttgggacctt tactttatgg ggaagttgga 6420

gacacactgt tgattatatt taagaatcaa gcaagcagac catataacat ctaccctcac 6480

ggaatcactg atgtccgtcc tttgtattca aggagattac caaaaggtgt aaaacatttg 6540

aaggattttc caattctgcc aggagaaata ttcaaatata aatggacagt gactgtagaa 6600

gatgggccaa ctaaatcaga tcctcggtgc ctgacccgct attactctag tttcgttaat 6660

atggagagag atctagcttc aggactcatt ggccctctcc tcatctgcta caaagaatct 6720

gtagatcaaa gaggaaacca gataatgtca gacaagagga atgtcatcct gttttctgta 6780

tttgatgaga accgaagctg gtacctcaca gagaatatac aacgctttct ccccaatcca 6840

gctggagtgc agcttgagga tccagagttc caagcctcca acatcatgca cagcatcaat 6900

ggctatgttt ttgatagttt gcagttgtca gtttgtttgc atgaggtggc atactggtac 6960

attctaagca ttggagcaca gactgacttc ctttctgtct tcttctctgg atataccttc 7020

aaacacaaaa tggtctatga agacacactc accctattcc cattctcagg agaaactgtc 7080

ttcatgtcga tggaaaaccc aggtctatgg attctggggt gccacaactc agactttcgg 7140

aacagaggca tgaccgcctt actgaaggtt tctagttgtg acaagaacac tggtgattat 7200

tacgaggaca gttatgaaga tatttcagca tacttgctga gtaaaaacaa tgccattgaa 7260

ccaagaagct tctcccagaa ttcaagacac cctagccaaa acccaccagt cttgaaacgc 7320

catcaacggg aaataactcg tactactctt cagtcagatc aagaggaaat tgactatgat 7380

gataccatat cagttgaaat gaagaaggaa gattttgaca tttatgatga ggatgaaaat 7440

cagagccccc gcagctttca aaagaaaaca cgacactatt ttattgctgc agtggagagg 7500

ctctgggatt atgggatgag tagctcccca catgttctaa gaaacagggc tcagagtggc 7560

agtgtccctc agttcaagaa agttgttttc caggaattta ctgatggctc ctttactcag 7620

cccttatacc gtggagaact aaatgaacat ttgggactcc tggggccata tataagagca 7680

gaagttgaag ataatatcat ggtaactttc agaaatcagg cctctcgtcc ctattccttc 7740

tattctagcc ttatttctta tgaggaagat cagaggcaag gagcagaacc tagaaaaaac 7800

tttgtcaagc ctaatgaaac caaaacttac ttttggaaag tgcaacatca tatggcaccc 7860

actaaagatg agtttgactg caaagcctgg gcttatttct ctgatgttga cctggaaaaa 7920

gatgtgcact caggcctgat tggacccctt ctggtctgcc acactaacac actgaaccct 7980

gctcatggga gacaagtgac agtacaggaa tttgctctgt ttttcaccat ctttgatgag 8040

accaaaagct ggtacttcac tgaaaatatg gaaagaaact gcagggctcc ctgcaatatc 8100

cagatggaag atcccacttt taaagagaat tatcgcttcc atgcaatcaa tggctacata 8160

atggatacac tacctggctt agtaatggct caggatcaaa ggattcgatg gtatctgctc 8220

agcatgggca gcaatgaaaa catccattct attcatttca gtggacatgt gttcactgta 8280

cgaaaaaaag aggagtataa aatggcactg tacaatctct atccaggtgt ttttgagaca 8340

gtggaaatgt taccatccaa agctggaatt tggcgggtgg aatgccttat tggcgagcat 8400

ctacatgctg ggatgagcac actttttctg gtgtacagca ataagtgtca gactcccctg 8460

ggaatggctt ctggacacat tagagatttt cagattacag cttcaggaca atatggacag 8520

tgggccccaa agctggccag acttcattat tccggatcaa tcaatgcctg gagcaccaag 8580

gagccctttt cttggatcaa ggtggatctg ttggcaccaa tgattattca cggcatcaag 8640

acccagggtg cccgtcagaa gttctccagc ctctacatct ctcagtttat catcatgtat 8700

agtcttgatg ggaagaagtg gcagacttat cgaggaaatt ccactggaac cttaatggtc 8760

ttctttggca atgtggattc atctgggata aaacacaata tttttaaccc tccaattatt 8820

gctcgataca tccgtttgca cccaactcat tatagcattc gcagcactct tcgcatggag 8880

ttgatgggct gtgatttaaa tagttgcagc atgccattgg gaatggagag taaagcaata 8940

tcagatgcac agattactgc ttcatcctac tttaccaata tgtttgccac ctggtctcct 9000

tcaaaagctc gacttcacct ccaagggagg agtaatgcct ggagacctca ggtgaataat 9060

ccaaaagagt ggctgcaagt ggacttccag aagacaatga aagtcacagg agtaactact 9120

cagggagtaa aatctctgct taccagcatg tatgtgaagg agttcctcat ctccagcagt 9180

caagatggcc atcagtggac tctctttttt cagaatggca aagtaaaggt ttttcaggga 9240

aatcaagact ccttcacacc tgtggtgaac tctctagacc caccgttact gactcgctac 9300

cttcgaattc acccccagag ttgggtgcac cagattgccc tgaggatgga ggttctgggc 9360

tgcgaggcac aggacctcta ctgagcggcc gcgtttaaac gtcgacaatc aacctctgga 9420

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 9480

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 9540

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 9600

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 9660

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 9720

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 9780

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 9840

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 9900

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 9960

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 10020

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10080

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10140

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 10200

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10260

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 10320

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 10380

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10440

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10500

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 10560

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10620

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 10680

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 10740

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 10800

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 10860

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 10920

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 10980

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 11040

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11100

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11160

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11220

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 11280

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 11340

c 11341

<210> 8

<211> 4392

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

atgcagattg agctgtccac ctgtttcttc ctgtgcctgc tgagattttg cttcagtgct 60

acaaggagat actacctggg ggctgtcgag ctgtcttggg attacatgca gtctgatctg 120

ggcgaactgc cagtggacgc gcggtttcct ccaagggtgc caaagtcctt cccctttaat 180

acatctgtgg tgtacaagaa gaccctgttt gtggagttta ccgaccacct gtttaacatc 240

gcgaagccta gaccaccctg gatgggcctg ctgggcccca caatccaggc cgaagtgtat 300

gacacagtgg taatcacact gaagaacatg gccagccacc cagtgtccct gcacgcggtg 360

ggcgtatcct actggaaggc cagcgaaggc gcggagtatg atgaccagac atcccagaga 420

gagaaggagg atgacaaggt gtttccaggc gggtcccaca cctatgtatg gcaggtgctg 480

aaggagaatg gccccatggc ctccgacccc ctgtgcctga catacagcta tctgtcccac 540

gtagacctgg tgaaggatct gaattccggg ctgatcgggg ccctgctggt gtgcagggag 600

ggctccctgg ccaaggagaa gacccagacc ctgcacaagt ttatcctgct gttcgcggtg 660

tttgatgagg gcaagtcctg gcacagcgaa acaaagaact ctctgatgca ggacagggac 720

gcggccagcg cgcgggcctg gccaaagatg cacaccgtaa atggctatgt gaacaggtcc 780

ctgccaggcc tgatcgggtg ccacagaaag tctgtatatt ggcacgtaat cgggatgggc 840

accacaccag aggtgcactc catctttctg gagggccaca ccttcctggt gagaaaccac 900

aggcaggcca gcctggagat cagccccatc acattcctga cagcccagac cctgctgatg 960

gacctgggcc agtttctgct gttttgtcac atcagctccc accagcacga tggcatggag 1020

gcctatgtga aggtggatag ctgccctgag gagccacagc tgaggatgaa gaataatgag 1080

gaggccgaag attatgatga tgacctgacc gacagcgaaa tggatgtggt gaggtttgac 1140

gatgacaact ccccatcctt catccagatc cgctccgtag ccaagaagca ccctaagaca 1200

tgggtgcact atatcgcggc cgaagaggag gactgggatt atgccccact ggtgctggcc 1260

cctgacgatc ggagctacaa gtcccagtat ctgaataatg gcccccagag aatcgggcgg 1320

aagtacaaga aggtgagatt catggcctat accgatgaga cattcaagac cagggaggcc 1380

atccagcacg aatctggcat cctgggccct ctgctgtatg gcgaagtggg cgacacactg 1440

ctgatcatct tcaagaacca ggccagcaga ccatacaaca tctacccaca cgggatcaca 1500

gacgtacggc ctctgtacag ccgccgcctg ccaaagggcg taaagcacct gaaggatttc 1560

cctatcctgc ccggggagat ctttaagtat aagtggacag tgacagtgga ggatggccca 1620

accaagtccg atccaaggtg cctgaccaga tactacagct cctttgtgaa catggagaga 1680

gatctggcca gcgggctgat cgggccactg ctgatctgct acaaggagag cgtagatcag 1740

agaggcaatc agatcatgtc cgacaagagg aatgtgatcc tgttctctgt attcgatgag 1800

aatagatctt ggtacctgac agagaacatc cagagatttc tgccaaatcc agccggggtg 1860

cagctggagg acccagagtt tcaggcctct aacatcatgc actccatcaa tggctatgta 1920

tttgactctc tgcagctgag cgtatgtctg cacgaagtgg cctactggta catcctgtcc 1980

atcggggccc agacagactt cctgtctgta ttcttttctg gctacacatt caagcacaag 2040

atggtgtatg aggacaccct gaccctgttt cctttctccg gggagaccgt attcatgtct 2100

atggagaatc ctggcctgtg gatactgggc tgtcacaatt ctgactttag aaatagaggc 2160

atgacagccc tgctgaaggt gtcctcctgt gacaagaata caggcgacta ctatgaggat 2220

tcctatgagg atatcagcgc gtatctgctg agcaagaata atgccatcga acctaggtct 2280

ttttctcaga actccagaca cccctcccag aaccctcctg tgctgaagag acaccagaga 2340

gagatcacaa ggaccaccct gcagtctgac caggaggaga tcgactatga tgatacaatc 2400

tctgtggaga tgaagaagga ggattttgat atctatgacg aagatgagaa tcagagccca 2460

agatccttcc agaagaagac ccgccactat ttcatcgcgg ccgtagagag actgtgggac 2520

tatggcatgt cttcctctcc ccacgtactg aggaacagag cccagtctgg ctctgtgcct 2580

cagtttaaga aggtggtgtt ccaggagttc acagacggga gctttaccca gccactgtac 2640

agaggcgaac tgaatgagca cctgggcctg ctgggcccat acatcagggc cgaagtggag 2700

gataacatca tggtgacctt cagaaatcag gccagcaggc catactcctt ttactcctcc 2760

ctgatctctt atgaggagga tcagagacag ggcgcggagc caagaaagaa ttttgtgaag 2820

ccaaatgaga caaagacata cttttggaag gtgcagcacc acatggcccc aaccaaggac 2880

gaatttgatt gcaaggcctg ggcctatttt tccgacgtgg atctggagaa ggatgtgcac 2940

tctggcctga tcgggcccct gctggtgtgc cacacaaaca ccctgaatcc tgcccacggg 3000

cgccaggtga cagtgcagga gtttgccctg ttctttacca tctttgacga aacaaagtcc 3060

tggtacttca cagagaacat ggagagaaat tgcagggccc catgcaatat ccagatggag 3120

gaccccacct tcaaggagaa ttatagattt cacgcgatca atggctacat catggatacc 3180

ctgccaggcc tggtaatggc ccaggatcag agaatcagat ggtacctgct gtctatgggc 3240

tctaatgaga acatccactc catccacttc tccgggcacg tattcaccgt aaggaagaag 3300

gaggagtaca agatggccct gtacaatctg tatccaggcg tatttgagac agtggagatg 3360

ctgcccagca aggccgggat ctggagagtg gagtgtctga tcggggagca cctgcacgcg 3420

ggcatgtcca cactgtttct ggtgtactcc aataagtgcc agacccctct gggcatggcc 3480

agcgggcaca tccgggactt ccagatcacc gcgtctggcc agtatggcca gtgggccccc 3540

aagctggccc gcctgcacta tagcgggtct atcaacgcgt ggtccaccaa ggagcccttt 3600

tcctggatca aggtggacct gctggcccca atgatcatcc acgggatcaa gacccagggc 3660

gcgcgccaga agttttcttc tctgtacatc tcccagttta tcatcatgta ctccctggat 3720

ggcaagaagt ggcagacata tcgcggcaac tccacaggca ccctgatggt gttctttggc 3780

aatgtggact cctccgggat caagcacaac atctttaatc caccaatcat cgcgcggtat 3840

atcaggctgc accccaccca ctactctatc agaagcacac tgaggatgga gctgatgggc 3900

tgtgacctga attcctgttc catgcctctg ggcatggaga gcaaggccat ctctgatgcc 3960

cagatcacag ccagctctta tttcacaaac atgtttgcca cctggtcccc atctaaggcc 4020

agactgcacc tgcagggcag atccaatgcc tggagaccac aggtgaataa tcccaaggag 4080

tggctgcagg tggacttcca gaagacaatg aaggtgaccg gggtgaccac ccagggcgta 4140

aagtccctgc tgacatccat gtatgtgaag gagttcctga tcagctcttc ccaggatggc 4200

caccagtgga ccctgttctt tcagaatggc aaggtgaagg tgtttcaggg caatcaggat 4260

tcctttaccc ctgtggtgaa ttccctggac ccacctctgc tgaccagata tctgagaatc 4320

caccctcaga gctgggtcca ccagattgcc ctgagaatgg aagtgctggg atgtgaagct 4380

caggatctgt at 4392

<210> 9

<211> 11341

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 9

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga 3000

aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 3060

gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 3120

gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 3180

ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 3240

gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 3300

aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 3360

ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 3420

aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 3480

ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 3540

aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 3600

gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 3660

gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta 3720

ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 3780

ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 3840

agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 3900

accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 3960

cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 4020

ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 4080

aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 4140

ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 4200

gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 4260

aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga 4320

tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg 4380

attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 4440

aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatggggg aggctgctgg 4500

tgaatattaa ccaaggtcac cccagttatc ggaggagcaa acaggggcta agtccactct 4560

tgcatctaaa atgagagaca aaaaatctat aaaaatggaa aacatgcata gaaatatgtg 4620

agggaggaaa aaattacccc caagaatgtt agtgcacgca gtcacacagg gagaagacta 4680

tttttgtttt gttttgattg ttttgttttg ttttggttgt tttgttttgg tgacctaact 4740

ggtcaaatga cctattaaga atatttcata gaacgaatgt tccgatgctc taatctctct 4800

agacaaggtt catatttgta tgggttactt attctctctt tgttgactaa gtcaataatc 4860

agaatcagca ggtttgcagt cagattggca gggataagca gcctagctca ggagaagtga 4920

gtataaaagc cccaggctgg gagcagccat cacagaagtc cactcattct tggatccacc 4980

ggtgccacca tgcagattga gctgtccacc tgtttcttcc tgtgcctgct gagattttgc 5040

ttcagtgcta caaggagata ctacctgggg gctgtcgagc tgtcttggga ttacatgcag 5100

tctgatctgg gcgaactgcc agtggacgcg cggtttcctc caagggtgcc aaagtccttc 5160

ccctttaata catctgtggt gtacaagaag accctgtttg tggagtttac cgaccacctg 5220

tttaacatcg cgaagcctag accaccctgg atgggcctgc tgggccccac aatccaggcc 5280

gaagtgtatg acacagtggt aatcacactg aagaacatgg ccagccaccc agtgtccctg 5340

cacgcggtgg gcgtatccta ctggaaggcc agcgaaggcg cggagtatga tgaccagaca 5400

tcccagagag agaaggagga tgacaaggtg tttccaggcg ggtcccacac ctatgtatgg 5460

caggtgctga aggagaatgg ccccatggcc tccgaccccc tgtgcctgac atacagctat 5520

ctgtcccacg tagacctggt gaaggatctg aattccgggc tgatcggggc cctgctggtg 5580

tgcagggagg gctccctggc caaggagaag acccagaccc tgcacaagtt tatcctgctg 5640

ttcgcggtgt ttgatgaggg caagtcctgg cacagcgaaa caaagaactc tctgatgcag 5700

gacagggacg cggccagcgc gcgggcctgg ccaaagatgc acaccgtaaa tggctatgtg 5760

aacaggtccc tgccaggcct gatcgggtgc cacagaaagt ctgtatattg gcacgtaatc 5820

gggatgggca ccacaccaga ggtgcactcc atctttctgg agggccacac cttcctggtg 5880

agaaaccaca ggcaggccag cctggagatc agccccatca cattcctgac agcccagacc 5940

ctgctgatgg acctgggcca gtttctgctg ttttgtcaca tcagctccca ccagcacgat 6000

ggcatggagg cctatgtgaa ggtggatagc tgccctgagg agccacagct gaggatgaag 6060

aataatgagg aggccgaaga ttatgatgat gacctgaccg acagcgaaat ggatgtggtg 6120

aggtttgacg atgacaactc cccatccttc atccagatcc gctccgtagc caagaagcac 6180

cctaagacat gggtgcacta tatcgcggcc gaagaggagg actgggatta tgccccactg 6240

gtgctggccc ctgacgatcg gagctacaag tcccagtatc tgaataatgg cccccagaga 6300

atcgggcgga agtacaagaa ggtgagattc atggcctata ccgatgagac attcaagacc 6360

agggaggcca tccagcacga atctggcatc ctgggccctc tgctgtatgg cgaagtgggc 6420

gacacactgc tgatcatctt caagaaccag gccagcagac catacaacat ctacccacac 6480

gggatcacag acgtacggcc tctgtacagc cgccgcctgc caaagggcgt aaagcacctg 6540

aaggatttcc ctatcctgcc cggggagatc tttaagtata agtggacagt gacagtggag 6600

gatggcccaa ccaagtccga tccaaggtgc ctgaccagat actacagctc ctttgtgaac 6660

atggagagag atctggccag cgggctgatc gggccactgc tgatctgcta caaggagagc 6720

gtagatcaga gaggcaatca gatcatgtcc gacaagagga atgtgatcct gttctctgta 6780

ttcgatgaga atagatcttg gtacctgaca gagaacatcc agagatttct gccaaatcca 6840

gccggggtgc agctggagga cccagagttt caggcctcta acatcatgca ctccatcaat 6900

ggctatgtat ttgactctct gcagctgagc gtatgtctgc acgaagtggc ctactggtac 6960

atcctgtcca tcggggccca gacagacttc ctgtctgtat tcttttctgg ctacacattc 7020

aagcacaaga tggtgtatga ggacaccctg accctgtttc ctttctccgg ggagaccgta 7080

ttcatgtcta tggagaatcc tggcctgtgg atactgggct gtcacaattc tgactttaga 7140

aatagaggca tgacagccct gctgaaggtg tcctcctgtg acaagaatac aggcgactac 7200

tatgaggatt cctatgagga tatcagcgcg tatctgctga gcaagaataa tgccatcgaa 7260

cctaggtctt tttctcagaa ctccagacac ccctcccaga accctcctgt gctgaagaga 7320

caccagagag agatcacaag gaccaccctg cagtctgacc aggaggagat cgactatgat 7380

gatacaatct ctgtggagat gaagaaggag gattttgata tctatgacga agatgagaat 7440

cagagcccaa gatccttcca gaagaagacc cgccactatt tcatcgcggc cgtagagaga 7500

ctgtgggact atggcatgtc ttcctctccc cacgtactga ggaacagagc ccagtctggc 7560

tctgtgcctc agtttaagaa ggtggtgttc caggagttca cagacgggag ctttacccag 7620

ccactgtaca gaggcgaact gaatgagcac ctgggcctgc tgggcccata catcagggcc 7680

gaagtggagg ataacatcat ggtgaccttc agaaatcagg ccagcaggcc atactccttt 7740

tactcctccc tgatctctta tgaggaggat cagagacagg gcgcggagcc aagaaagaat 7800

tttgtgaagc caaatgagac aaagacatac ttttggaagg tgcagcacca catggcccca 7860

accaaggacg aatttgattg caaggcctgg gcctattttt ccgacgtgga tctggagaag 7920

gatgtgcact ctggcctgat cgggcccctg ctggtgtgcc acacaaacac cctgaatcct 7980

gcccacgggc gccaggtgac agtgcaggag tttgccctgt tctttaccat ctttgacgaa 8040

acaaagtcct ggtacttcac agagaacatg gagagaaatt gcagggcccc atgcaatatc 8100

cagatggagg accccacctt caaggagaat tatagatttc acgcgatcaa tggctacatc 8160

atggataccc tgccaggcct ggtaatggcc caggatcaga gaatcagatg gtacctgctg 8220

tctatgggct ctaatgagaa catccactcc atccacttct ccgggcacgt attcaccgta 8280

aggaagaagg aggagtacaa gatggccctg tacaatctgt atccaggcgt atttgagaca 8340

gtggagatgc tgcccagcaa ggccgggatc tggagagtgg agtgtctgat cggggagcac 8400

ctgcacgcgg gcatgtccac actgtttctg gtgtactcca ataagtgcca gacccctctg 8460

ggcatggcca gcgggcacat ccgggacttc cagatcaccg cgtctggcca gtatggccag 8520

tgggccccca agctggcccg cctgcactat agcgggtcta tcaacgcgtg gtccaccaag 8580

gagccctttt cctggatcaa ggtggacctg ctggccccaa tgatcatcca cgggatcaag 8640

acccagggcg cgcgccagaa gttttcttct ctgtacatct cccagtttat catcatgtac 8700

tccctggatg gcaagaagtg gcagacatat cgcggcaact ccacaggcac cctgatggtg 8760

ttctttggca atgtggactc ctccgggatc aagcacaaca tctttaatcc accaatcatc 8820

gcgcggtata tcaggctgca ccccacccac tactctatca gaagcacact gaggatggag 8880

ctgatgggct gtgacctgaa ttcctgttcc atgcctctgg gcatggagag caaggccatc 8940

tctgatgccc agatcacagc cagctcttat ttcacaaaca tgtttgccac ctggtcccca 9000

tctaaggcca gactgcacct gcagggcaga tccaatgcct ggagaccaca ggtgaataat 9060

cccaaggagt ggctgcaggt ggacttccag aagacaatga aggtgaccgg ggtgaccacc 9120

cagggcgtaa agtccctgct gacatccatg tatgtgaagg agttcctgat cagctcttcc 9180

caggatggcc accagtggac cctgttcttt cagaatggca aggtgaaggt gtttcagggc 9240

aatcaggatt cctttacccc tgtggtgaat tccctggacc cacctctgct gaccagatat 9300

ctgagaatcc accctcagag ctgggtccac cagattgccc tgagaatgga agtgctggga 9360

tgtgaagctc aggatctgta ttgagcggcc gcgtttaaac gtcgacaatc aacctctgga 9420

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 9480

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 9540

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 9600

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 9660

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 9720

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 9780

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 9840

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 9900

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 9960

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 10020

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10080

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10140

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 10200

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10260

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 10320

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 10380

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10440

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10500

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 10560

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10620

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 10680

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 10740

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 10800

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 10860

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 10920

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 10980

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 11040

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11100

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11160

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11220

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 11280

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 11340

c 11341

<210> 10

<211> 4392

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 10

atgcagatcg agctgtccac ctgtttcttc ctgtgcctgc tgcgcttctg tttctccgcc 60

acccgacgat actacctggg ggccgtggag ctgagctggg actacatgca gagcgacctg 120

ggcgagctgc ccgtggacgc ccggttcccc cccagggtgc ccaagagctt ccccttcaac 180

accagcgtgg tgtacaagaa gaccctgttc gtggagttca ccgaccacct gttcaacatc 240

gccaagccca ggcccccctg gatgggcctg ctgggcccca ccatccaggc cgaggtgtac 300

gacaccgtgg tcatcaccct gaagaacatg gccagccacc ccgtgagcct gcacgccgtg 360

ggcgtgagct actggaaggc cagcgagggc gccgagtacg acgaccagac cagccagagg 420

gagaaggagg acgacaaggt gttccccggc ggcagccaca cctacgtgtg gcaggtgctg 480

aaggagaacg gccccatggc cagcgacccc ctgtgcctga cctacagcta cctgagccac 540

gtggacctgg tgaaggacct gaacagcggc ctgatcggcg ccctgctggt gtgcagggag 600

ggcagcctgg ccaaggagaa gacccagacc ctgcacaagt tcatcctgct gttcgccgtg 660

ttcgacgagg gcaagagctg gcacagcgag acgaagaaca gcctgatgca ggacagggac 720

gccgccagcg ccagggcctg gcccaagatg cacaccgtga acggctacgt gaaccggagc 780

ctgcccggcc tgatcggctg ccacaggaag agcgtgtact ggcacgtgat cggcatgggc 840

accacccccg aggtgcacag catcttcctg gagggccaca ccttcctggt gcggaaccac 900

aggcaggcca gcctggagat cagccccatc accttcctga ccgcccagac cctgctgatg 960

gacctgggcc agttcctgct gttctgccac atcagcagcc accagcacga cggcatggag 1020

gcctacgtga aggtggacag ctgccccgag gagccccagc tgcggatgaa gaacaacgag 1080

gaggccgagg actacgacga cgacctgacc gacagcgaga tggacgtggt gcggttcgac 1140

gacgacaaca gccccagctt catccagatc aggagcgtgg ccaagaagca ccccaagacc 1200

tgggtgcact acatcgccgc cgaggaggag gactgggact acgcccccct ggtgctggcc 1260

cccgacgaca ggagctacaa gagccagtac ctgaacaacg gcccccagcg gatcggcagg 1320

aagtacaaga aggtgcggtt catggcctac accgacgaga cattcaagac cagggaggcc 1380

atccagcacg agagcggcat cctgggcccc ctgctgtacg gcgaggtcgg cgacaccctg 1440

ctgatcatct tcaagaacca ggccagccgg ccctacaaca tctaccccca cggcatcacc 1500

gacgtgaggc ccctgtacag ccggaggctg cccaagggcg tgaagcacct gaaggacttc 1560

cccatcctgc ccggcgagat cttcaagtac aagtggaccg tgaccgtgga ggacggcccc 1620

accaagagcg acccccggtg cctgaccagg tactacagca gcttcgtgaa catggagagg 1680

gacctggcca gcggcctgat cggccccctg ctgatctgct acaaggagag cgtggaccag 1740

cggggcaacc agatcatgag cgacaagagg aacgtgatcc tgttcagcgt gttcgacgag 1800

aaccggagct ggtacctgac cgagaacatc cagaggttcc tgcccaaccc cgccggcgtg 1860

cagctggagg accccgagtt ccaggccagc aacatcatgc acagcatcaa cggctacgtg 1920

ttcgacagcc tgcagctgag cgtgtgcctg cacgaggtgg cctactggta catcctgagc 1980

atcggcgccc agaccgactt cctgagcgtg ttcttcagcg gctacacctt caagcacaag 2040

atggtgtacg aggacaccct gaccctgttc cccttcagcg gcgagacggt gttcatgagc 2100

atggagaacc ccggcctgtg gatactgggc tgccacaaca gcgacttccg gaacaggggc 2160

atgaccgccc tgctgaaggt gagcagctgc gacaagaaca ccggcgacta ctacgaggac 2220

agctacgagg acatcagcgc ctacctgctg agcaagaaca acgccatcga gccccggagc 2280

ttcagccaga acagcaggca ccccagccag aacccccccg tgctgaagag gcaccagagg 2340

gagatcacca ggaccaccct gcagagcgac caggaggaga tcgactacga cgacaccatc 2400

agcgtggaga tgaagaagga ggacttcgac atctacgacg aggacgagaa ccagagcccc 2460

cggagcttcc agaagaagac caggcactac ttcatcgccg ccgtggagag gctgtgggac 2520

tacggcatga gcagcagccc ccacgtgctg aggaacaggg cccagagcgg cagcgtgccc 2580

cagttcaaga aggtggtgtt ccaggagttc accgacggca gcttcaccca gcccctgtac 2640

aggggcgagc tgaacgagca cctgggcctg ctgggcccct acatcagggc cgaggtggag 2700

gacaacatca tggtgacctt ccggaaccag gccagcaggc cctacagctt ctacagcagc 2760

ctgatcagct acgaggagga ccagaggcag ggcgccgagc ccaggaagaa cttcgtgaag 2820

cccaacgaga cgaagaccta cttctggaag gtgcagcacc acatggcccc caccaaggac 2880

gagttcgact gcaaggcctg ggcctacttc agcgacgtgg acctggagaa ggacgtgcac 2940

tccggcctga tcgggcccct gctggtgtgc cacaccaaca ccctgaaccc cgcccacggc 3000

cggcaggtga ccgtgcagga gttcgccctg ttcttcacca tcttcgacga gacgaagagc 3060

tggtacttca ccgagaacat ggagcggaac tgcagggccc cctgcaacat ccagatggag 3120

gaccccacct tcaaggagaa ctacaggttc cacgccatca acggctacat catggacacc 3180

ctgcccggcc tggtcatggc ccaggaccag cggatcaggt ggtacctgct gagcatgggc 3240

agcaacgaga acatccacag catccacttc agcggccacg tgttcaccgt gcggaagaag 3300

gaggagtaca agatggccct gtacaacctg taccccggcg tgttcgagac ggtggagatg 3360

ctgcccagca aggccggcat ctggagggtg gagtgcctga tcggcgagca cctgcacgcc 3420

ggcatgagca ccctgttcct ggtgtacagc aacaagtgcc agacccccct gggcatggcc 3480

agcggccaca tccgggactt ccagatcacc gccagcggcc agtacggcca gtgggccccc 3540

aagctggcca ggctgcacta cagcggcagc atcaacgcct ggagcaccaa ggagcccttc 3600

agctggatca aggtggacct gctggccccc atgatcatcc acggcatcaa gacccagggc 3660

gccaggcaga agttcagcag cctgtacatc agccagttca tcatcatgta cagcctggac 3720

ggcaagaagt ggcagaccta caggggcaac agcaccggca ccctgatggt gttcttcggc 3780

aacgtggaca gcagcggcat caagcacaac atcttcaacc cccccatcat cgcccggtac 3840

atcaggctgc accccaccca ctacagcatc cggagcaccc tgaggatgga gctgatgggc 3900

tgcgacctga acagctgcag catgcccctg ggcatggaga gcaaggccat cagcgacgcc 3960

cagatcaccg ccagcagcta cttcaccaac atgttcgcca cctggagccc cagcaaggcc 4020

aggctgcacc tgcagggccg gagcaacgcc tggaggcccc aggtgaacaa ccccaaggag 4080

tggctgcagg tggacttcca gaagaccatg aaggtgaccg gcgtgaccac ccagggcgtg 4140

aagagcctgc tgaccagcat gtacgtgaag gagttcctga tcagcagcag ccaggacggc 4200

caccagtgga ccctgttctt ccagaacggc aaggtgaagg tgttccaggg caaccaggac 4260

agcttcaccc ccgtggtgaa cagcctggac ccccccctgc tgaccaggta cctgcgcatc 4320

cacccccaga gctgggtcca ccagatcgcc ctgaggatgg aggtgctggg gtgtgaggcc 4380

caggacctgt at 4392

<210> 11

<211> 11341

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 11

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga 3000

aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 3060

gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 3120

gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 3180

ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 3240

gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 3300

aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 3360

ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 3420

aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 3480

ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 3540

aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 3600

gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 3660

gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta 3720

ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 3780

ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 3840

agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 3900

accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 3960

cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 4020

ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 4080

aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 4140

ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 4200

gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 4260

aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga 4320

tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg 4380

attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 4440

aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatggggg aggctgctgg 4500

tgaatattaa ccaaggtcac cccagttatc ggaggagcaa acaggggcta agtccactct 4560

tgcatctaaa atgagagaca aaaaatctat aaaaatggaa aacatgcata gaaatatgtg 4620

agggaggaaa aaattacccc caagaatgtt agtgcacgca gtcacacagg gagaagacta 4680

tttttgtttt gttttgattg ttttgttttg ttttggttgt tttgttttgg tgacctaact 4740

ggtcaaatga cctattaaga atatttcata gaacgaatgt tccgatgctc taatctctct 4800

agacaaggtt catatttgta tgggttactt attctctctt tgttgactaa gtcaataatc 4860

agaatcagca ggtttgcagt cagattggca gggataagca gcctagctca ggagaagtga 4920

gtataaaagc cccaggctgg gagcagccat cacagaagtc cactcattct tggatccacc 4980

ggtgccacca tgcagatcga gctgtccacc tgtttcttcc tgtgcctgct gcgcttctgt 5040

ttctccgcca cccgacgata ctacctgggg gccgtggagc tgagctggga ctacatgcag 5100

agcgacctgg gcgagctgcc cgtggacgcc cggttccccc ccagggtgcc caagagcttc 5160

cccttcaaca ccagcgtggt gtacaagaag accctgttcg tggagttcac cgaccacctg 5220

ttcaacatcg ccaagcccag gcccccctgg atgggcctgc tgggccccac catccaggcc 5280

gaggtgtacg acaccgtggt catcaccctg aagaacatgg ccagccaccc cgtgagcctg 5340

cacgccgtgg gcgtgagcta ctggaaggcc agcgagggcg ccgagtacga cgaccagacc 5400

agccagaggg agaaggagga cgacaaggtg ttccccggcg gcagccacac ctacgtgtgg 5460

caggtgctga aggagaacgg ccccatggcc agcgaccccc tgtgcctgac ctacagctac 5520

ctgagccacg tggacctggt gaaggacctg aacagcggcc tgatcggcgc cctgctggtg 5580

tgcagggagg gcagcctggc caaggagaag acccagaccc tgcacaagtt catcctgctg 5640

ttcgccgtgt tcgacgaggg caagagctgg cacagcgaga cgaagaacag cctgatgcag 5700

gacagggacg ccgccagcgc cagggcctgg cccaagatgc acaccgtgaa cggctacgtg 5760

aaccggagcc tgcccggcct gatcggctgc cacaggaaga gcgtgtactg gcacgtgatc 5820

ggcatgggca ccacccccga ggtgcacagc atcttcctgg agggccacac cttcctggtg 5880

cggaaccaca ggcaggccag cctggagatc agccccatca ccttcctgac cgcccagacc 5940

ctgctgatgg acctgggcca gttcctgctg ttctgccaca tcagcagcca ccagcacgac 6000

ggcatggagg cctacgtgaa ggtggacagc tgccccgagg agccccagct gcggatgaag 6060

aacaacgagg aggccgagga ctacgacgac gacctgaccg acagcgagat ggacgtggtg 6120

cggttcgacg acgacaacag ccccagcttc atccagatca ggagcgtggc caagaagcac 6180

cccaagacct gggtgcacta catcgccgcc gaggaggagg actgggacta cgcccccctg 6240

gtgctggccc ccgacgacag gagctacaag agccagtacc tgaacaacgg cccccagcgg 6300

atcggcagga agtacaagaa ggtgcggttc atggcctaca ccgacgagac attcaagacc 6360

agggaggcca tccagcacga gagcggcatc ctgggccccc tgctgtacgg cgaggtcggc 6420

gacaccctgc tgatcatctt caagaaccag gccagccggc cctacaacat ctacccccac 6480

ggcatcaccg acgtgaggcc cctgtacagc cggaggctgc ccaagggcgt gaagcacctg 6540

aaggacttcc ccatcctgcc cggcgagatc ttcaagtaca agtggaccgt gaccgtggag 6600

gacggcccca ccaagagcga cccccggtgc ctgaccaggt actacagcag cttcgtgaac 6660

atggagaggg acctggccag cggcctgatc ggccccctgc tgatctgcta caaggagagc 6720

gtggaccagc ggggcaacca gatcatgagc gacaagagga acgtgatcct gttcagcgtg 6780

ttcgacgaga accggagctg gtacctgacc gagaacatcc agaggttcct gcccaacccc 6840

gccggcgtgc agctggagga ccccgagttc caggccagca acatcatgca cagcatcaac 6900

ggctacgtgt tcgacagcct gcagctgagc gtgtgcctgc acgaggtggc ctactggtac 6960

atcctgagca tcggcgccca gaccgacttc ctgagcgtgt tcttcagcgg ctacaccttc 7020

aagcacaaga tggtgtacga ggacaccctg accctgttcc ccttcagcgg cgagacggtg 7080

ttcatgagca tggagaaccc cggcctgtgg atactgggct gccacaacag cgacttccgg 7140

aacaggggca tgaccgccct gctgaaggtg agcagctgcg acaagaacac cggcgactac 7200

tacgaggaca gctacgagga catcagcgcc tacctgctga gcaagaacaa cgccatcgag 7260

ccccggagct tcagccagaa cagcaggcac cccagccaga acccccccgt gctgaagagg 7320

caccagaggg agatcaccag gaccaccctg cagagcgacc aggaggagat cgactacgac 7380

gacaccatca gcgtggagat gaagaaggag gacttcgaca tctacgacga ggacgagaac 7440

cagagccccc ggagcttcca gaagaagacc aggcactact tcatcgccgc cgtggagagg 7500

ctgtgggact acggcatgag cagcagcccc cacgtgctga ggaacagggc ccagagcggc 7560

agcgtgcccc agttcaagaa ggtggtgttc caggagttca ccgacggcag cttcacccag 7620

cccctgtaca ggggcgagct gaacgagcac ctgggcctgc tgggccccta catcagggcc 7680

gaggtggagg acaacatcat ggtgaccttc cggaaccagg ccagcaggcc ctacagcttc 7740

tacagcagcc tgatcagcta cgaggaggac cagaggcagg gcgccgagcc caggaagaac 7800

ttcgtgaagc ccaacgagac gaagacctac ttctggaagg tgcagcacca catggccccc 7860

accaaggacg agttcgactg caaggcctgg gcctacttca gcgacgtgga cctggagaag 7920

gacgtgcact ccggcctgat cgggcccctg ctggtgtgcc acaccaacac cctgaacccc 7980

gcccacggcc ggcaggtgac cgtgcaggag ttcgccctgt tcttcaccat cttcgacgag 8040

acgaagagct ggtacttcac cgagaacatg gagcggaact gcagggcccc ctgcaacatc 8100

cagatggagg accccacctt caaggagaac tacaggttcc acgccatcaa cggctacatc 8160

atggacaccc tgcccggcct ggtcatggcc caggaccagc ggatcaggtg gtacctgctg 8220

agcatgggca gcaacgagaa catccacagc atccacttca gcggccacgt gttcaccgtg 8280

cggaagaagg aggagtacaa gatggccctg tacaacctgt accccggcgt gttcgagacg 8340

gtggagatgc tgcccagcaa ggccggcatc tggagggtgg agtgcctgat cggcgagcac 8400

ctgcacgccg gcatgagcac cctgttcctg gtgtacagca acaagtgcca gacccccctg 8460

ggcatggcca gcggccacat ccgggacttc cagatcaccg ccagcggcca gtacggccag 8520

tgggccccca agctggccag gctgcactac agcggcagca tcaacgcctg gagcaccaag 8580

gagcccttca gctggatcaa ggtggacctg ctggccccca tgatcatcca cggcatcaag 8640

acccagggcg ccaggcagaa gttcagcagc ctgtacatca gccagttcat catcatgtac 8700

agcctggacg gcaagaagtg gcagacctac aggggcaaca gcaccggcac cctgatggtg 8760

ttcttcggca acgtggacag cagcggcatc aagcacaaca tcttcaaccc ccccatcatc 8820

gcccggtaca tcaggctgca ccccacccac tacagcatcc ggagcaccct gaggatggag 8880

ctgatgggct gcgacctgaa cagctgcagc atgcccctgg gcatggagag caaggccatc 8940

agcgacgccc agatcaccgc cagcagctac ttcaccaaca tgttcgccac ctggagcccc 9000

agcaaggcca ggctgcacct gcagggccgg agcaacgcct ggaggcccca ggtgaacaac 9060

cccaaggagt ggctgcaggt ggacttccag aagaccatga aggtgaccgg cgtgaccacc 9120

cagggcgtga agagcctgct gaccagcatg tacgtgaagg agttcctgat cagcagcagc 9180

caggacggcc accagtggac cctgttcttc cagaacggca aggtgaaggt gttccagggc 9240

aaccaggaca gcttcacccc cgtggtgaac agcctggacc cccccctgct gaccaggtac 9300

ctgcgcatcc acccccagag ctgggtccac cagatcgccc tgaggatgga ggtgctgggg 9360

tgtgaggccc aggacctgta ttgagcggcc gcgtttaaac gtcgacaatc aacctctgga 9420

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 9480

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 9540

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 9600

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 9660

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 9720

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 9780

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 9840

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 9900

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 9960

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 10020

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10080

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10140

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 10200

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10260

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 10320

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 10380

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10440

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10500

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 10560

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10620

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 10680

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 10740

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 10800

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 10860

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 10920

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 10980

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 11040

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11100

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11160

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11220

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 11280

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 11340

c 11341

<210> 12

<211> 1182

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 12

gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 60

gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 120

atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 180

tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg 240

tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta 300

cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg 360

tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc 420

tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctccct 480

gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc 540

tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg 600

gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg 660

cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg 720

cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca 780

ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg 840

aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt 900

ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc 960

gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg 1020

atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg 1080

taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag 1140

acagtggttc aaagtttttt tcttccattt caggtgtcgt ga 1182

<210> 13

<211> 7896

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 13

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga 3000

aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa 3060

gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg 3120

agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa 3180

aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa 3240

gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta 3300

gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat 3360

tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca 3420

aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag 3480

cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta 3540

tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga 3600

agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg 3660

ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa 3720

ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag 3780

catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg 3840

gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt 3900

tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg 3960

aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac 4020

tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta 4080

gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa 4140

ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct 4200

atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc 4260

ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac 4320

agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg 4380

gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa 4440

actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatga gtaattcata 4500

caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 4560

tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 4620

gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 4680

cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 4740

gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 4800

gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 4860

ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 4920

gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc 4980

gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 5040

cctcgtgctt gagttgaggc ctggcttggg cgctggggcc gccgcgtgcg aatctggtgg 5100

caccttcgcg cctgtctccc tgctttcgat aagtctctag ccatttaaaa tttttgatga 5160

cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 5220

actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 5280

tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 5340

gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 5400

gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 5460

gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 5520

acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 5580

cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 5640

tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 5700

aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 5760

tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 5820

tgaggatcta tttccggtga gacccaagct ggctagctaa acttacgcgt gcctcggatc 5880

ctccagtgtg gtgtgcagat atccagcaca gtcccgggcc gagtctagac gtttaaaccc 5940

gctgatcagg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 6000

cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 6060

gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 6120

ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 6180

gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 6240

gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 6300

acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 6360

tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 6420

gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 6480

cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 6540

ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 6600

ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 6660

aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 6720

tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 6780

aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 6840

agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 6900

aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 6960

gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 7020

ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 7080

gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 7140

tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 7200

ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt 7260

gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 7320

ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 7380

gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac 7440

gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 7500

acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 7560

ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 7620

gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 7680

tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 7740

ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 7800

gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 7860

gcgaatttta acaaaatatt aacgtttaca atttcc 7896

<210> 14

<211> 12061

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 14

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga 3000

aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 3060

gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 3120

gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 3180

ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 3240

gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 3300

aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 3360

ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 3420

aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 3480

ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 3540

aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 3600

gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 3660

gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta 3720

ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 3780

ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 3840

agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 3900

accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 3960

cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 4020

ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 4080

aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 4140

ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 4200

gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 4260

aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga 4320

tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg 4380

attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 4440

aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagag catgcgtgag 4500

gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 4560

gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 4620

atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 4680

tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg 4740

tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta 4800

cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg 4860

tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc 4920

tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctccct 4980

gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc 5040

tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg 5100

gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg 5160

cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg 5220

cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca 5280

ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg 5340

aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt 5400

ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc 5460

gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg 5520

atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg 5580

taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag 5640

acagtggttc aaagtttttt tcttccattt caggtgtcgt gactcattct tggatccacc 5700

ggtgccacca tgcagatcga actgagtacc tgcttctttc tttgcttgtt gcgcttttgc 5760

ttttctgcga cgcggcggta ttacttgggg gcggttgagt tgagctggga ttatatgcaa 5820

tctgacctgg gtgaactgcc ggtcgatgcg cggttcccgc cccgggttcc caaaagtttt 5880

ccattcaaca catccgtcgt ttacaaaaaa acgcttttcg tagaattcac agatcacctg 5940

tttaacatag cgaagccacg acctccctgg atgggccttt tgggacctac gatacaagct 6000

gaagtatacg ataccgttgt tatcacactg aagaacatgg caagtcaccc tgtttccctt 6060

catgccgtgg gagtatcata ttggaaggct tctgaaggag cagaatatga tgatcaaaca 6120

agtcaaagag agaaggaaga tgacaaagtg ttccctgggg ggagtcatac gtacgtgtgg 6180

caagtattga aggaaaatgg tccgatggcg tctgacccgc tttgtcttac ctattcctac 6240

ctttctcacg tggacctggt aaaagatctg aactcaggtc tcattggcgc cctgttggtt 6300

tgtcgcgagg gttcattggc aaaagaaaag actcaaacgc ttcacaagtt tatccttctc 6360

tttgccgtct tcgatgaagg gaagtcttgg catagtgaga ctaagaactc cctcatgcaa 6420

gacagggatg ctgcatccgc gcgagcgtgg cctaagatgc acacggttaa cggctatgtg 6480

aacaggagcc tgccagggct catcggttgc cacaggaagt ccgtgtactg gcatgttata 6540

gggatgggga ctacacctga agtccattct atattcctcg aaggacacac ctttcttgta 6600

cgaaatcacc gccaagcgtc tcttgaaatt tcccctatta ccttcctcac tgcacaaacc 6660

cttctgatgg acctgggcca atttcttctg ttctgtcaca ttagttcaca tcaacatgac 6720

ggtatggagg cttacgtcaa ggtggacagc tgcccagagg aaccccaatt gcgcatgaaa 6780

aacaatgagg aagctgaaga ctacgacgat gatcttacgg actccgagat ggacgtggtt 6840

cgctttgatg atgataattc tccttctttc atccaaatcc gatctgtggc aaaaaaacat 6900

cccaagacgt gggtgcatta tatcgctgcg gaggaagaag attgggacta tgctcctttg 6960

gtgcttgcac ctgatgaccg cagttataag tcacagtact tgaataacgg ccctcaaaga 7020

atcggaagaa aatataagaa ggtccgattc atggcctaca ccgacgagac gttcaaaacc 7080

cgagaagcta ttcagcacga aagcggaata ctggggccgc tgttgtatgg tgaagtcgga 7140

gatacacttc tcataatatt taaaaaccag gcttcacggc catacaacat ctatccgcat 7200

ggtatcaccg acgtgcggcc cctgtatagt cggagactgc ctaagggggt aaaacatctc 7260

aaggattttc cgattctccc cggagaaatt ttcaagtata aatggacggt gacggtcgag 7320

gatggtccta ccaaatccga tccccggtgt ctcacaagat actacagcag cttcgttaat 7380

atggaaagag acctcgcttc cggacttatc ggaccgttgc tcatatgtta caaagagtcc 7440

gtagatcaaa ggggcaacca aattatgtcc gataagcgga atgttatatt gttcagtgtc 7500

ttcgacgaga acaggtcttg gtatttgact gaaaacatcc agcgatttct gccgaacccc 7560

gcaggggtac aattggagga cccggaattc caagctagta atatcatgca ttctatcaat 7620

ggatacgtat ttgattccct tcagcttagc gtttgtctgc atgaagtcgc atattggtat 7680

atccttagta ttggtgctca aactgacttc ctgtctgtat ttttttctgg ttacaccttc 7740

aagcacaaga tggtctacga ggacactctt acgctttttc ccttctctgg agagacggtg 7800

tttatgagca tggaaaaccc cgggctttgg attctcgggt gccataattc agacttccgc 7860

aacaggggta tgacagcctt gttgaaggtc agctcctgcg ataaaaacac cggggattac 7920

tatgaagact cctacgaaga catttctgca tatctcctct ccaaaaacaa cgcgatcgaa 7980

ccaaggtctt tttcccagaa ctcacggcat ccaagccaga atccaccagt gttgaaacgc 8040

catcagaggg aaattacgcg aacgaccttg caaagcgatc aggaagaaat tgattatgac 8100

gacactataa gtgtagaaat gaaaaaagag gactttgaca tctatgatga ggatgagaac 8160

cagtccccaa gaagttttca gaagaagacc cgccactatt ttattgctgc cgtcgaacgg 8220

ttgtgggact acggaatgag ctcctccccg catgtgttgc ggaatcgagc ccaaagtggc 8280

tctgtgcctc agttcaaaaa ggtcgtattc caagaattca ctgatggcag cttcactcag 8340

ccactgtatc ggggggagtt gaacgaacat ctcggcctct tgggcccata catacgcgct 8400

gaggttgaag ataacataat ggtaactttt cgaaatcagg catcaaggcc ttattcattt 8460

tacagctctc tcatatctta cgaagaggac caaagacaag gagcggaacc tcgcaagaat 8520

tttgtaaaac ccaatgaaac gaaaacgtat ttctggaagg ttcagcacca catggcccca 8580

acaaaggatg aatttgattg taaagcgtgg gcgtatttta gtgacgtcga tctcgaaaag 8640

gatgttcatt cagggcttat cggtcccctc cttgtgtgtc atacaaacac acttaatccg 8700

gcgcacggta gacaagtaac tgtgcaggaa tttgcgttgt ttttcacgat ctttgatgaa 8760

actaagtcat ggtattttac ggagaacatg gagcggaatt gtagggcacc atgtaatata 8820

cagatggaag acccaacctt taaagaaaat tacagattcc atgccataaa cgggtacatc 8880

atggatactc tcccaggact ggtaatggct caggaccagc gaatacgatg gtacttgctt 8940

agcatgggga gtaacgaaaa catccattct attcattttt caggccatgt gttcactgtc 9000

agaaaaaagg aggagtataa gatggcgctc tacaatctgt accccggtgt gtttgagacg 9060

gtagaaatgc tgccctccaa agctggtata tggagagtag agtgtttgat aggagaacat 9120

ctccacgccg gcatgtctac gctgtttctc gtttacagca ataagtgcca gacccccctg 9180

gggatggcta gtgggcacat ccgcgatttt caaattacag catctgggca atacggtcaa 9240

tgggcgccga aactggctag actgcattat tccgggtcca ttaatgcttg gtccactaag 9300

gagcccttca gctggatcaa ggtagacctt ctcgcgccta tgattataca cggtataaag 9360

acccaaggtg ccagacagaa gtttagtagc ctttacatat cacagtttat tataatgtac 9420

tccttggatg gcaagaagtg gcaaacctat aggggtaact ccacgggaac cctgatggtc 9480

tttttcggga acgtagactc ctcaggaata aagcacaata ttttcaatcc cccaatcata 9540

gcgcgctata tacgacttca tcctacgcat tactccatac gctctacgct gcgaatggag 9600

ctgatgggct gcgatctgaa cagttgctcc atgcctctgg gcatggaatc taaagccatc 9660

agcgatgcac aaattaccgc tagtagctac ttcaccaata tgtttgccac atggtccccg 9720

tctaaggctc gcctgcatct gcaaggccgg tccaacgcat ggcgacctca ggtcaataac 9780

ccaaaggaat ggttgcaggt agactttcag aagaccatga aggttaccgg ggtaactact 9840

cagggggtaa aatcactgtt gactagcatg tacgtgaaag aattcctcat tagcagtagt 9900

caagatggcc atcagtggac gctgttcttt caaaacggga aggtcaaagt tttccagggg 9960

aatcaggact ccttcacacc cgtcgtcaac tcactcgatc caccactgtt gacccggtac 10020

ctgagaatcc acccacaatc ctgggttcac caaatcgcac tcaggatgga agtactcggg 10080

tgcgaagcgc aggacctcta ctgagcggcc gcgtttaaac gtcgacaatc aacctctgga 10140

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 10200

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 10260

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 10320

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 10380

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 10440

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 10500

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 10560

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 10620

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 10680

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 10740

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10800

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10860

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 10920

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10980

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 11040

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 11100

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 11160

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 11220

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 11280

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 11340

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 11400

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 11460

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 11520

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 11580

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 11640

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 11700

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 11760

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11820

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11880

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11940

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 12000

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 12060

c 12061

<210> 15

<211> 12061

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 15

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga 3000

aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 3060

gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 3120

gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 3180

ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 3240

gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 3300

aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 3360

ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 3420

aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 3480

ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 3540

aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 3600

gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 3660

gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta 3720

ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 3780

ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 3840

agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 3900

accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 3960

cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 4020

ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 4080

aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 4140

ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 4200

gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 4260

aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga 4320

tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg 4380

attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 4440

aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagag catgcgtgag 4500

gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 4560

gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 4620

atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 4680

tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg 4740

tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta 4800

cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg 4860

tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc 4920

tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctccct 4980

gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc 5040

tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg 5100

gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg 5160

cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg 5220

cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca 5280

ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg 5340

aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt 5400

ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc 5460

gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg 5520

atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg 5580

taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag 5640

acagtggttc aaagtttttt tcttccattt caggtgtcgt gactcattct tggatccacc 5700

ggtgccacca tgcagattga gctgtccacc tgtttcttcc tgtgcctgct gagattttgc 5760

ttcagtgcta caaggagata ctacctgggg gctgtcgagc tgtcttggga ttacatgcag 5820

tctgatctgg gcgaactgcc agtggacgcg cggtttcctc caagggtgcc aaagtccttc 5880

ccctttaata catctgtggt gtacaagaag accctgtttg tggagtttac cgaccacctg 5940

tttaacatcg cgaagcctag accaccctgg atgggcctgc tgggccccac aatccaggcc 6000

gaagtgtatg acacagtggt aatcacactg aagaacatgg ccagccaccc agtgtccctg 6060

cacgcggtgg gcgtatccta ctggaaggcc agcgaaggcg cggagtatga tgaccagaca 6120

tcccagagag agaaggagga tgacaaggtg tttccaggcg ggtcccacac ctatgtatgg 6180

caggtgctga aggagaatgg ccccatggcc tccgaccccc tgtgcctgac atacagctat 6240

ctgtcccacg tagacctggt gaaggatctg aattccgggc tgatcggggc cctgctggtg 6300

tgcagggagg gctccctggc caaggagaag acccagaccc tgcacaagtt tatcctgctg 6360

ttcgcggtgt ttgatgaggg caagtcctgg cacagcgaaa caaagaactc tctgatgcag 6420

gacagggacg cggccagcgc gcgggcctgg ccaaagatgc acaccgtaaa tggctatgtg 6480

aacaggtccc tgccaggcct gatcgggtgc cacagaaagt ctgtatattg gcacgtaatc 6540

gggatgggca ccacaccaga ggtgcactcc atctttctgg agggccacac cttcctggtg 6600

agaaaccaca ggcaggccag cctggagatc agccccatca cattcctgac agcccagacc 6660

ctgctgatgg acctgggcca gtttctgctg ttttgtcaca tcagctccca ccagcacgat 6720

ggcatggagg cctatgtgaa ggtggatagc tgccctgagg agccacagct gaggatgaag 6780

aataatgagg aggccgaaga ttatgatgat gacctgaccg acagcgaaat ggatgtggtg 6840

aggtttgacg atgacaactc cccatccttc atccagatcc gctccgtagc caagaagcac 6900

cctaagacat gggtgcacta tatcgcggcc gaagaggagg actgggatta tgccccactg 6960

gtgctggccc ctgacgatcg gagctacaag tcccagtatc tgaataatgg cccccagaga 7020

atcgggcgga agtacaagaa ggtgagattc atggcctata ccgatgagac attcaagacc 7080

agggaggcca tccagcacga atctggcatc ctgggccctc tgctgtatgg cgaagtgggc 7140

gacacactgc tgatcatctt caagaaccag gccagcagac catacaacat ctacccacac 7200

gggatcacag acgtacggcc tctgtacagc cgccgcctgc caaagggcgt aaagcacctg 7260

aaggatttcc ctatcctgcc cggggagatc tttaagtata agtggacagt gacagtggag 7320

gatggcccaa ccaagtccga tccaaggtgc ctgaccagat actacagctc ctttgtgaac 7380

atggagagag atctggccag cgggctgatc gggccactgc tgatctgcta caaggagagc 7440

gtagatcaga gaggcaatca gatcatgtcc gacaagagga atgtgatcct gttctctgta 7500

ttcgatgaga atagatcttg gtacctgaca gagaacatcc agagatttct gccaaatcca 7560

gccggggtgc agctggagga cccagagttt caggcctcta acatcatgca ctccatcaat 7620

ggctatgtat ttgactctct gcagctgagc gtatgtctgc acgaagtggc ctactggtac 7680

atcctgtcca tcggggccca gacagacttc ctgtctgtat tcttttctgg ctacacattc 7740

aagcacaaga tggtgtatga ggacaccctg accctgtttc ctttctccgg ggagaccgta 7800

ttcatgtcta tggagaatcc tggcctgtgg atactgggct gtcacaattc tgactttaga 7860

aatagaggca tgacagccct gctgaaggtg tcctcctgtg acaagaatac aggcgactac 7920

tatgaggatt cctatgagga tatcagcgcg tatctgctga gcaagaataa tgccatcgaa 7980

cctaggtctt tttctcagaa ctccagacac ccctcccaga accctcctgt gctgaagaga 8040

caccagagag agatcacaag gaccaccctg cagtctgacc aggaggagat cgactatgat 8100

gatacaatct ctgtggagat gaagaaggag gattttgata tctatgacga agatgagaat 8160

cagagcccaa gatccttcca gaagaagacc cgccactatt tcatcgcggc cgtagagaga 8220

ctgtgggact atggcatgtc ttcctctccc cacgtactga ggaacagagc ccagtctggc 8280

tctgtgcctc agtttaagaa ggtggtgttc caggagttca cagacgggag ctttacccag 8340

ccactgtaca gaggcgaact gaatgagcac ctgggcctgc tgggcccata catcagggcc 8400

gaagtggagg ataacatcat ggtgaccttc agaaatcagg ccagcaggcc atactccttt 8460

tactcctccc tgatctctta tgaggaggat cagagacagg gcgcggagcc aagaaagaat 8520

tttgtgaagc caaatgagac aaagacatac ttttggaagg tgcagcacca catggcccca 8580

accaaggacg aatttgattg caaggcctgg gcctattttt ccgacgtgga tctggagaag 8640

gatgtgcact ctggcctgat cgggcccctg ctggtgtgcc acacaaacac cctgaatcct 8700

gcccacgggc gccaggtgac agtgcaggag tttgccctgt tctttaccat ctttgacgaa 8760

acaaagtcct ggtacttcac agagaacatg gagagaaatt gcagggcccc atgcaatatc 8820

cagatggagg accccacctt caaggagaat tatagatttc acgcgatcaa tggctacatc 8880

atggataccc tgccaggcct ggtaatggcc caggatcaga gaatcagatg gtacctgctg 8940

tctatgggct ctaatgagaa catccactcc atccacttct ccgggcacgt attcaccgta 9000

aggaagaagg aggagtacaa gatggccctg tacaatctgt atccaggcgt atttgagaca 9060

gtggagatgc tgcccagcaa ggccgggatc tggagagtgg agtgtctgat cggggagcac 9120

ctgcacgcgg gcatgtccac actgtttctg gtgtactcca ataagtgcca gacccctctg 9180

ggcatggcca gcgggcacat ccgggacttc cagatcaccg cgtctggcca gtatggccag 9240

tgggccccca agctggcccg cctgcactat agcgggtcta tcaacgcgtg gtccaccaag 9300

gagccctttt cctggatcaa ggtggacctg ctggccccaa tgatcatcca cgggatcaag 9360

acccagggcg cgcgccagaa gttttcttct ctgtacatct cccagtttat catcatgtac 9420

tccctggatg gcaagaagtg gcagacatat cgcggcaact ccacaggcac cctgatggtg 9480

ttctttggca atgtggactc ctccgggatc aagcacaaca tctttaatcc accaatcatc 9540

gcgcggtata tcaggctgca ccccacccac tactctatca gaagcacact gaggatggag 9600

ctgatgggct gtgacctgaa ttcctgttcc atgcctctgg gcatggagag caaggccatc 9660

tctgatgccc agatcacagc cagctcttat ttcacaaaca tgtttgccac ctggtcccca 9720

tctaaggcca gactgcacct gcagggcaga tccaatgcct ggagaccaca ggtgaataat 9780

cccaaggagt ggctgcaggt ggacttccag aagacaatga aggtgaccgg ggtgaccacc 9840

cagggcgtaa agtccctgct gacatccatg tatgtgaagg agttcctgat cagctcttcc 9900

caggatggcc accagtggac cctgttcttt cagaatggca aggtgaaggt gtttcagggc 9960

aatcaggatt cctttacccc tgtggtgaat tccctggacc cacctctgct gaccagatat 10020

ctgagaatcc accctcagag ctgggtccac cagattgccc tgagaatgga agtgctggga 10080

tgtgaagctc aggatctgta ttgagcggcc gcgtttaaac gtcgacaatc aacctctgga 10140

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 10200

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 10260

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 10320

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 10380

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 10440

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 10500

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 10560

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 10620

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 10680

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 10740

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10800

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10860

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 10920

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10980

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 11040

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 11100

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 11160

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 11220

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 11280

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 11340

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 11400

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 11460

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 11520

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 11580

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 11640

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 11700

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 11760

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11820

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11880

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11940

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 12000

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 12060

c 12061

<210> 16

<211> 12061

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 16

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 60

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 120

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 180

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 240

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 300

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 360

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 420

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 480

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 540

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 600

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 660

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 720

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 780

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 840

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 900

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 960

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1020

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1080

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1140

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1200

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1260

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1320

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1380

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1440

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1500

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1560

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1620

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1680

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1740

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1800

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1860

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1920

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1980

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2040

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2100

gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 2160

tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 2220

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 2280

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 2340

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 2400

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 2460

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 2520

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 2580

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 2640

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 2700

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 2760

ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc 2820

tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2880

gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2940

cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga 3000

aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 3060

gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 3120

gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 3180

ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 3240

gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 3300

aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 3360

ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 3420

aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 3480

ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 3540

aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 3600

gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 3660

gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta 3720

ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 3780

ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 3840

agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 3900

accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 3960

cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 4020

ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 4080

aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 4140

ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 4200

gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 4260

aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga 4320

tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg 4380

attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 4440

aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagag catgcgtgag 4500

gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 4560

gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 4620

atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 4680

tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg 4740

tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta 4800

cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg 4860

tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc 4920

tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctccct 4980

gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc 5040

tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg 5100

gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg 5160

cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg 5220

cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca 5280

ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg 5340

aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt 5400

ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc 5460

gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg 5520

atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg 5580

taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag 5640

acagtggttc aaagtttttt tcttccattt caggtgtcgt gactcattct tggatccacc 5700

ggtgccacca tgcagatcga gctgtccacc tgtttcttcc tgtgcctgct gcgcttctgt 5760

ttctccgcca cccgacgata ctacctgggg gccgtggagc tgagctggga ctacatgcag 5820

agcgacctgg gcgagctgcc cgtggacgcc cggttccccc ccagggtgcc caagagcttc 5880

cccttcaaca ccagcgtggt gtacaagaag accctgttcg tggagttcac cgaccacctg 5940

ttcaacatcg ccaagcccag gcccccctgg atgggcctgc tgggccccac catccaggcc 6000

gaggtgtacg acaccgtggt catcaccctg aagaacatgg ccagccaccc cgtgagcctg 6060

cacgccgtgg gcgtgagcta ctggaaggcc agcgagggcg ccgagtacga cgaccagacc 6120

agccagaggg agaaggagga cgacaaggtg ttccccggcg gcagccacac ctacgtgtgg 6180

caggtgctga aggagaacgg ccccatggcc agcgaccccc tgtgcctgac ctacagctac 6240

ctgagccacg tggacctggt gaaggacctg aacagcggcc tgatcggcgc cctgctggtg 6300

tgcagggagg gcagcctggc caaggagaag acccagaccc tgcacaagtt catcctgctg 6360

ttcgccgtgt tcgacgaggg caagagctgg cacagcgaga cgaagaacag cctgatgcag 6420

gacagggacg ccgccagcgc cagggcctgg cccaagatgc acaccgtgaa cggctacgtg 6480

aaccggagcc tgcccggcct gatcggctgc cacaggaaga gcgtgtactg gcacgtgatc 6540

ggcatgggca ccacccccga ggtgcacagc atcttcctgg agggccacac cttcctggtg 6600

cggaaccaca ggcaggccag cctggagatc agccccatca ccttcctgac cgcccagacc 6660

ctgctgatgg acctgggcca gttcctgctg ttctgccaca tcagcagcca ccagcacgac 6720

ggcatggagg cctacgtgaa ggtggacagc tgccccgagg agccccagct gcggatgaag 6780

aacaacgagg aggccgagga ctacgacgac gacctgaccg acagcgagat ggacgtggtg 6840

cggttcgacg acgacaacag ccccagcttc atccagatca ggagcgtggc caagaagcac 6900

cccaagacct gggtgcacta catcgccgcc gaggaggagg actgggacta cgcccccctg 6960

gtgctggccc ccgacgacag gagctacaag agccagtacc tgaacaacgg cccccagcgg 7020

atcggcagga agtacaagaa ggtgcggttc atggcctaca ccgacgagac attcaagacc 7080

agggaggcca tccagcacga gagcggcatc ctgggccccc tgctgtacgg cgaggtcggc 7140

gacaccctgc tgatcatctt caagaaccag gccagccggc cctacaacat ctacccccac 7200

ggcatcaccg acgtgaggcc cctgtacagc cggaggctgc ccaagggcgt gaagcacctg 7260

aaggacttcc ccatcctgcc cggcgagatc ttcaagtaca agtggaccgt gaccgtggag 7320

gacggcccca ccaagagcga cccccggtgc ctgaccaggt actacagcag cttcgtgaac 7380

atggagaggg acctggccag cggcctgatc ggccccctgc tgatctgcta caaggagagc 7440

gtggaccagc ggggcaacca gatcatgagc gacaagagga acgtgatcct gttcagcgtg 7500

ttcgacgaga accggagctg gtacctgacc gagaacatcc agaggttcct gcccaacccc 7560

gccggcgtgc agctggagga ccccgagttc caggccagca acatcatgca cagcatcaac 7620

ggctacgtgt tcgacagcct gcagctgagc gtgtgcctgc acgaggtggc ctactggtac 7680

atcctgagca tcggcgccca gaccgacttc ctgagcgtgt tcttcagcgg ctacaccttc 7740

aagcacaaga tggtgtacga ggacaccctg accctgttcc ccttcagcgg cgagacggtg 7800

ttcatgagca tggagaaccc cggcctgtgg atactgggct gccacaacag cgacttccgg 7860

aacaggggca tgaccgccct gctgaaggtg agcagctgcg acaagaacac cggcgactac 7920

tacgaggaca gctacgagga catcagcgcc tacctgctga gcaagaacaa cgccatcgag 7980

ccccggagct tcagccagaa cagcaggcac cccagccaga acccccccgt gctgaagagg 8040

caccagaggg agatcaccag gaccaccctg cagagcgacc aggaggagat cgactacgac 8100

gacaccatca gcgtggagat gaagaaggag gacttcgaca tctacgacga ggacgagaac 8160

cagagccccc ggagcttcca gaagaagacc aggcactact tcatcgccgc cgtggagagg 8220

ctgtgggact acggcatgag cagcagcccc cacgtgctga ggaacagggc ccagagcggc 8280

agcgtgcccc agttcaagaa ggtggtgttc caggagttca ccgacggcag cttcacccag 8340

cccctgtaca ggggcgagct gaacgagcac ctgggcctgc tgggccccta catcagggcc 8400

gaggtggagg acaacatcat ggtgaccttc cggaaccagg ccagcaggcc ctacagcttc 8460

tacagcagcc tgatcagcta cgaggaggac cagaggcagg gcgccgagcc caggaagaac 8520

ttcgtgaagc ccaacgagac gaagacctac ttctggaagg tgcagcacca catggccccc 8580

accaaggacg agttcgactg caaggcctgg gcctacttca gcgacgtgga cctggagaag 8640

gacgtgcact ccggcctgat cgggcccctg ctggtgtgcc acaccaacac cctgaacccc 8700

gcccacggcc ggcaggtgac cgtgcaggag ttcgccctgt tcttcaccat cttcgacgag 8760

acgaagagct ggtacttcac cgagaacatg gagcggaact gcagggcccc ctgcaacatc 8820

cagatggagg accccacctt caaggagaac tacaggttcc acgccatcaa cggctacatc 8880

atggacaccc tgcccggcct ggtcatggcc caggaccagc ggatcaggtg gtacctgctg 8940

agcatgggca gcaacgagaa catccacagc atccacttca gcggccacgt gttcaccgtg 9000

cggaagaagg aggagtacaa gatggccctg tacaacctgt accccggcgt gttcgagacg 9060

gtggagatgc tgcccagcaa ggccggcatc tggagggtgg agtgcctgat cggcgagcac 9120

ctgcacgccg gcatgagcac cctgttcctg gtgtacagca acaagtgcca gacccccctg 9180

ggcatggcca gcggccacat ccgggacttc cagatcaccg ccagcggcca gtacggccag 9240

tgggccccca agctggccag gctgcactac agcggcagca tcaacgcctg gagcaccaag 9300

gagcccttca gctggatcaa ggtggacctg ctggccccca tgatcatcca cggcatcaag 9360

acccagggcg ccaggcagaa gttcagcagc ctgtacatca gccagttcat catcatgtac 9420

agcctggacg gcaagaagtg gcagacctac aggggcaaca gcaccggcac cctgatggtg 9480

ttcttcggca acgtggacag cagcggcatc aagcacaaca tcttcaaccc ccccatcatc 9540

gcccggtaca tcaggctgca ccccacccac tacagcatcc ggagcaccct gaggatggag 9600

ctgatgggct gcgacctgaa cagctgcagc atgcccctgg gcatggagag caaggccatc 9660

agcgacgccc agatcaccgc cagcagctac ttcaccaaca tgttcgccac ctggagcccc 9720

agcaaggcca ggctgcacct gcagggccgg agcaacgcct ggaggcccca ggtgaacaac 9780

cccaaggagt ggctgcaggt ggacttccag aagaccatga aggtgaccgg cgtgaccacc 9840

cagggcgtga agagcctgct gaccagcatg tacgtgaagg agttcctgat cagcagcagc 9900

caggacggcc accagtggac cctgttcttc cagaacggca aggtgaaggt gttccagggc 9960

aaccaggaca gcttcacccc cgtggtgaac agcctggacc cccccctgct gaccaggtac 10020

ctgcgcatcc acccccagag ctgggtccac cagatcgccc tgaggatgga ggtgctgggg 10080

tgtgaggccc aggacctgta ttgagcggcc gcgtttaaac gtcgacaatc aacctctgga 10140

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 10200

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 10260

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 10320

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 10380

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 10440

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 10500

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 10560

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 10620

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 10680

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 10740

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 10800

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 10860

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 10920

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 10980

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 11040

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 11100

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 11160

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 11220

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 11280

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 11340

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga 11400

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 11460

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 11520

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 11580

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 11640

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 11700

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 11760

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 11820

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 11880

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 11940

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 12000

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 12060

c 12061

<210> 17

<211> 5612

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 17

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 60

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 120

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 180

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 240

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 300

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 360

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 420

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 480

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 540

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 600

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 660

acgttaaggg attttggtca tgaagcgctt ttgaagctcg gatccgaaca aacgacccaa 720

cacccgtgcg ttttattctg tctttttatt gccgatcccc tcagaagaac tcgtcaagaa 780

ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 840

ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 900

gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 960

ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg 1020

gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 1080

ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 1140

gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 1200

catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 1260

ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 1320

ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt 1380

cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 1440

gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 1500

gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa 1560

acgatcctca tcctgtctct tgatcagagc ttgatcccct gcgccatcag atccttggcg 1620

gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag gcctgcgccg 1680

cggccagctg gctagcaatt cccgggttaa ctctagagac attgattatt gactagttat 1740

taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 1800

taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 1860

ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 1920

gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 1980

ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 2040

ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 2100

atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 2160

agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 2220

ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 2280

gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca 2340

cgctgttttg acctccatag aagacaccgg gaccgatcca gcctcccctc gaagcttaca 2400

tgtggtaccg agctcggatc ctgagaactt cagggtgagt ctatgggacc cttgatgttt 2460

tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa gtaacagggt 2520

acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct 2580

tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg 2640

gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat 2700

ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa 2760

ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt 2820

attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc 2880

atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg 2940

gcccatcact ttggcaaagc acgtgagatc tgaattctga cactatgaag tgccttttgt 3000

acttagcctt tttattcatt ggggtgaatt gcaagttcac catagttttt ccacacaacc 3060

aaaaaggaaa ctggaaaaat gttccttcta attaccatta ttgcccgtca agctcagatt 3120

taaattggca taatgactta ataggcacag ccttacaagt caaaatgccc aagagtcaca 3180

aggctattca agcagacggt tggatgtgtc atgcttccaa atgggtcact acttgtgatt 3240

tccgctggta tggaccgaag tatataacac attccatccg atccttcact ccatctgtag 3300

aacaatgcaa ggaaagcatt gaacaaacga aacaaggaac ttggctgaat ccaggcttcc 3360

ctcctcaaag ttgtggatat gcaactgtga cggatgccga agcagtgatt gtccaggtga 3420

ctcctcacca tgtgctggtt gatgaataca caggagaatg ggttgattca cagttcatca 3480

acggaaaatg cagcaattac atatgcccca ctgtccataa ctctacaacc tggcattctg 3540

actataaggt caaagggcta tgtgattcta acctcatttc catggacatc accttcttct 3600

cagaggacgg agagctatca tccctgggaa aggagggcac agggttcaga agtaactact 3660

ttgcttatga aactggaggc aaggcctgca aaatgcaata ctgcaagcat tggggagtca 3720

gactcccatc aggtgtctgg ttcgagatgg ctgataagga tctctttgct gcagccagat 3780

tccctgaatg cccagaaggg tcaagtatct ctgctccatc tcagacctca gtggatgtaa 3840

gtctaattca ggacgttgag aggatcttgg attattccct ctgccaagaa acctggagca 3900

aaatcagagc gggtcttcca atctctccag tggatctcag ctatcttgct cctaaaaacc 3960

caggaaccgg tcctgctttc accataatca atggtaccct aaaatacttt gagaccagat 4020

acatcagagt cgatattgct gctccaatcc tctcaagaat ggtcggaatg atcagtggaa 4080

ctaccacaga aagggaactg tgggatgact gggcaccata tgaagacgtg gaaattggac 4140

ccaatggagt tctgaggacc agttcaggat ataagtttcc tttatacatg attggacatg 4200

gtatgttgga ctccgatctt catcttagct caaaggctca ggtgttcgaa catcctcaca 4260

ttcaagacgc tgcttcgcaa cttcctgatg atgagagttt attttttggt gatactgggc 4320

tatccaaaaa tccaatcgag cttgtagaag gttggttcag tagttggaaa agctctattg 4380

cctctttttt ctttatcata gggttaatca ttggactatt cttggttctc cgagttggta 4440

tccatctttg cattaaatta aagcacacca agaaaagaca gatttataca gacatagaga 4500

tgaaccgact tggaaagtaa ctcaaatcct gcacaacaga ttcttcatgt ttggaccaaa 4560

tcaacttgtg ataccatgct caaagaggcc tcaattatat ttgagttttt aatttttatg 4620

aaaaaaaaaa aaaaaaacgg aattcacccc accagtgcag gctgcctatc agaaagtggt 4680

ggctggtgtg gctaatgccc tggcccacaa gtatcactaa gctcgctttc ttgctgtcca 4740

atttctatta aaggttcctt tgttccctaa gtccaactac taaactgggg gatattatga 4800

agggccttga gcatctggat tctgcctaat aaaaaacatt tattttcatt gcaatgatgt 4860

atttaaatta tttctgaata ttttactaaa aagggaatgt gggaggtcag tgcatttaaa 4920

acataaagaa atgaagagct agttcaaacc ttgggaaaat acactatatc ttaaactcca 4980

tgaaagaagg tgaggctgca aacagctaat gcacattggc aacagcccct gatgcctatg 5040

ccttattcat ccctcagaaa aggattcaag tagaggcttg atttggaggt taaagttttg 5100

ctatgctgta ttttagtcga ccattactta ttgttttagc tgtcctcatg aatgtctttt 5160

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta 5220

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct 5280

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga 5340

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac 5400

agtgacccgg aatccctcga catggcagtc tagcactagt gcggccgcag atctgcttcc 5460

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 5520

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 5580

aaaggccagc aaaaggccag gaaccgtaaa aa 5612

<210> 18

<211> 3387

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 18

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 60

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 120

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 180

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 240

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 300

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 360

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 420

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 480

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 540

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 600

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 660

acgttaaggg attttggtca tgaagcgctt ttgaagctcg gatccgaaca aacgacccaa 720

cacccgtgcg ttttattctg tctttttatt gccgatcccc tcagaagaac tcgtcaagaa 780

ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 840

ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 900

gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 960

ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg 1020

gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 1080

ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 1140

gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 1200

catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 1260

ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 1320

ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt 1380

cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 1440

gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 1500

gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa 1560

acgatcctca tcctgtctct tgatcagagc ttgatcccct gcgccatcag atccttggcg 1620

gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag gcctgcgccg 1680

cggccagctg gctagcaatt cccgggttaa ctctagagaa tgtagtctta tgcaatactc 1740

ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 1800

caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa 1860

cagacgggtc tgacatggat tggacgaacc actgaattcc gcattgcaga gatattgtat 1920

ttaagtgcct agctcgatac aataaacgcc atttgaccat tcaccacatt ggtgtgcacc 1980

tccaagctcg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt 2040

ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagcta gtcgattagg 2100

catctcctat ggcaggaaga agcggagaca gcgacgaaga cctcctcaag gcagtcagac 2160

tcatcaagtt tctctatcaa agcaacccac ctcccaatcc cgaggggacc cgacaggccc 2220

gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2280

ggatccttag cacttatctg ggacgatctg cggagcctgt gcctcttcag ctaccaccgc 2340

ttgagagact tactcttgat tgtaacgagg attgtggaac ttctgggacg cagggggtgg 2400

gaagccctca aatattggtg gaatctccta caatattgga gtcaggagct aaagaatagt 2460

gctgttagct tgctcaatgc cacagctata gcagtagctg aggggacaga tagggttata 2520

gaagtagtac aagaagcttg gcactggccg tcgttttaca acgtcgtgat ctgagcctgg 2580

gagatctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 2640

cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc aggaaaaccc 2700

tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 2760

cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg 2820

cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag 2880

caaccatagt gtcgaccatt acttattgtt ttagctgtcc tcatgaatgt cttttcacta 2940

cccatttgct tatcctgcat ctctcagcct tgactccact cagttctctt gcttagagat 3000

accacctttc ccctgaagtg ttccttccat gttttacggc gagatggttt ctcctcgcct 3060

ggccactcag ccttagttgt ctctgttgtc ttatagaggt ctacttgaag aaggaaaaac 3120

agggggcatg gtttgactgt cctgtgagcc cttcttccct gcctccccca ctcacagtga 3180

cccggaatcc ctcgacatgg cagtctagca ctagtgcggc cgcagatctg cttcctcgct 3240

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3300

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3360

ccagcaaaag gccaggaacc gtaaaaa 3387

<210> 19

<211> 9171

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 19

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 60

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 120

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 180

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 240

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 300

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 360

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 420

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 480

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 540

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 600

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 660

acgttaaggg attttggtca tgaagcgctt ttgaagctcg gatccgaaca aacgacccaa 720

cacccgtgcg ttttattctg tctttttatt gccgatcccc tcagaagaac tcgtcaagaa 780

ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 840

ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 900

gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 960

ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg 1020

gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 1080

ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 1140

gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 1200

catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 1260

ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 1320

ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt 1380

cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 1440

gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 1500

gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa 1560

acgatcctca tcctgtctct tgatcagagc ttgatcccct gcgccatcag atccttggcg 1620

gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag gcctgcgccg 1680

cggccagctg gctagcaatt cccgggttaa ctctagagac attgattatt gactagttat 1740

taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 1800

taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 1860

ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 1920

gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 1980

ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 2040

ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 2100

atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 2160

agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 2220

ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 2280

gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca 2340

cgctgttttg acctccatag aagacaccgg gaccgatcca gcctcccctc gaagcttaca 2400

tgtggtaccg agctcggatc ctgagaactt cagggtgagt ctatgggacc cttgatgttt 2460

tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa gtaacagggt 2520

acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct 2580

tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg 2640

gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat 2700

ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa 2760

ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt 2820

attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc 2880

atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg 2940

gcccatcact ttggcaaagc acgtgagatc tgaattcgag atctgccgcc gccatgggtg 3000

cgagagcgtc agtattaagc gggggagaat tagatcgatg ggaaaaaatt cggttaaggc 3060

cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac 3120

gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac 3180

agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag 3240

caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca 3300

agatagagga agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca gctgacacag 3360

gacacagcaa tcaggtcagc caaaattacc ctatagtgca gaacatccag gggcaaatgg 3420

tacatcaggc catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg 3480

ctttcagccc agaagtgata cccatgtttt cagcattatc agaaggagcc accccacaag 3540

atttaaacac catgctaaac acagtggggg gacatcaagc agccatgcaa atgttaaaag 3600

agaccatcaa tgaggaagct gcagaatggg atagagtgca tccagtgcat gcagggccta 3660

ttgcaccagg ccagatgaga gaaccaaggg gatcagacat cgctggaact actagtaccc 3720

ttcaggaaca aataggatgg atgacacata atccacctat cccagtagga gaaatctata 3780

aaagatggat aatcctggga ttaaataaaa tagtaagaat gtatagccct accagcattc 3840

tggacataag acaaggacca aaggaaccct ttagagacta tgtagaccga ttctataaaa 3900

ctctaagagc cgagcaagct tcacaagagg taaaaaattg gatgacagaa accttgttgg 3960

tccaaaatgc gaacccagat tgtaagacta ttttaaaagc attgggacca ggagcgacac 4020

tagaagaaat gatgacagca tgtcagggag tggggggacc cggccataaa gcaagagttt 4080

tggctgaagc aatgagccaa gtaacaaatc cagctaccat aatgatacag aaaggcaatt 4140

ttaggaacca aagaaagact gttaagtgtt tcaattgtgg caaagaaggg cacatagcca 4200

aaaattgcag ggcccctagg aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa 4260

tgaaagattg tactgagaga caggctaatt ttttagggaa gatctggcct tcccacaagg 4320

gaaggccagg gaattttctt cagagcagac cagagccaac agccccacca gaagagagct 4380

tcaggtttgg ggaagagaca acaactccct ctcagaagca ggagccgata gacaaggaac 4440

tgtatccttt agcttccctc agatcactct ttggcagcga cccctcgtca caataaagat 4500

aggggggcaa ttaaaggaag ctctattaga tactggtgct gacgacacag tattagaaga 4560

aatgaatttg ccaggaagat ggaaaccaaa aatgataggg ggaattggag gttttatcaa 4620

agtaagacag tatgatcaga tactcataga aatctgcgga cataaagcta taggtacagt 4680

attagtagga cctacacctg tcaacataat tggaagaaat ctgttgactc agattggctg 4740

cactttaaat tttcccatta gtcctattga gactgtacca gtaaaattaa agccaggaat 4800

ggatggccca aaagttaaac aatggccatt gacagaagaa aaaataaaag cattagtaga 4860

aatttgtaca gaaatggaaa aggaaggaaa aatttcaaaa attgggcctg aaaatccata 4920

caatactcca gtatttgcca taaagaaaaa agacagtact aaatggagaa aattagtaga 4980

tttcagagaa cttaataaga gaactcaaga tttctgggaa gttcaattag gaataccaca 5040

tcctgcaggg ttaaaacaga aaaaatcagt aacagtactg gatgtgggcg atgcatattt 5100

ttcagttccc ttagataaag acttcaggaa gtatactgca tttaccatac ctagtataaa 5160

caatgagaca ccagggatta gatatcagta caatgtgctt ccacagggat ggaaaggatc 5220

accagcaata ttccagtgta gcatgacaaa aatcttagag ccttttagaa aacaaaatcc 5280

agacatagtc atctatcaat acatggatga tttgtatgta ggatctgact tagaaatagg 5340

gcagcataga acaaaaatag aggaactgag acaacatctg ttgaggtggg gatttaccac 5400

accagacaaa aaacatcaga aagaacctcc attcctttgg atgggttatg aactccatcc 5460

tgataaatgg acagtacagc ctatagtgct gccagaaaag gacagctgga ctgtcaatga 5520

catacagaaa ttagtgggaa aattgaattg ggcaagtcag atttatgcag ggattaaagt 5580

aaggcaatta tgtaaacttc ttaggggaac caaagcacta acagaagtag taccactaac 5640

agaagaagca gagctagaac tggcagaaaa cagggagatt ctaaaagaac cggtacatgg 5700

agtgtattat gacccatcaa aagacttaat agcagaaata cagaagcagg ggcaaggcca 5760

atggacatat caaatttatc aagagccatt taaaaatctg aaaacaggaa agtatgcaag 5820

aatgaagggt gcccacacta atgatgtgaa acaattaaca gaggcagtac aaaaaatagc 5880

cacagaaagc atagtaatat ggggaaagac tcctaaattt aaattaccca tacaaaagga 5940

aacatgggaa gcatggtgga cagagtattg gcaagccacc tggattcctg agtgggagtt 6000

tgtcaatacc cctcccttag tgaagttatg gtaccagtta gagaaagaac ccataatagg 6060

agcagaaact ttctatgtag atggggcagc caatagggaa actaaattag gaaaagcagg 6120

atatgtaact gacagaggaa gacaaaaagt tgtcccccta acggacacaa caaatcagaa 6180

gactgagtta caagcaattc atctagcttt gcaggattcg ggattagaag taaacatagt 6240

gacagactca caatatgcat tgggaatcat tcaagcacaa ccagataaga gtgaatcaga 6300

gttagtcagt caaataatag agcagttaat aaaaaaggaa aaagtctacc tggcatgggt 6360

accagcacac aaaggaattg gaggaaatga acaagtagat aaattggtca gtgctggaat 6420

caggaaagta ctatttttag atggaataga taaggcccaa gaagaacatg agaaatatca 6480

cagtaattgg agagcaatgg ctagtgattt taacctacca cctgtagtag caaaagaaat 6540

agtagccagc tgtgataaat gtcagctaaa aggggaagcc atgcatggac aagtagactg 6600

tagcccagga atatggcagc tagattgtac acatttagaa ggaaaagtta tcttggtagc 6660

agttcatgta gccagtggat atatagaagc agaagtaatt ccagcagaga cagggcaaga 6720

aacagcatac ttcctcttaa aattagcagg aagatggcca gtaaaaacag tacatacaga 6780

caatggcagc aatttcacca gtactacagt taaggccgcc tgttggtggg cggggatcaa 6840

gcaggaattt ggcattccct acaatccgca gtcacaagga gtaatagaat ctatgaataa 6900

agaattaaag aaaattatag gacaggtaag agatcaggct gaacatctta aaacagcagt 6960

acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc 7020

aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca 7080

aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggaa 7140

aggaccagca aagctcctct ggaaaggtga aggggcagta gtaatacaag ataatagtga 7200

cataaaagta gtgccaagaa gaaaagcaaa gatcatcagg gattatggaa aacagatggc 7260

aggtgatgat tgtgtggcaa gtagacagga tgaggattaa cacatggaat tccggagcgg 7320

ccgcaggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt 7380

caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca 7440

atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca 7500

agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg 7560

ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt 7620

ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag 7680

aaattaacaa ttacacaagc ttccgcggaa ttcaccccac cagtgcaggc tgcctatcag 7740

aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc tcgctttctt 7800

gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta aactggggga 7860

tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta ttttcattgc 7920

aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg gaggtcagtg 7980

catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac actatatctt 8040

aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa cagcccctga 8100

tgcctatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat ttggaggtta 8160

aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca tgaatgtctt 8220

ttcactaccc atttgcttat cctgcatctc tcagccttga ctccactcag ttctcttgct 8280

tagagatacc acctttcccc tgaagtgttc cttccatgtt ttacggcgag atggtttctc 8340

ctcgcctggc cactcagcct tagttgtctc tgttgtctta tagaggtcta cttgaagaag 8400

gaaaaacagg gggcatggtt tgactgtcct gtgagccctt cttccctgcc tcccccactc 8460

acagtgaccc ggaatccctc gacatggcag tctagcacta gtgcggccgc agatctgctt 8520

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 8580

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 8640

caaaaggcca gcaaaaggcc aggaaccgta aaaagtcgac cattacttat tgttttagct 8700

gtcctcatga atgtcttttc actacccatt tgcttatcct gcatctctca gccttgactc 8760

cactcagttc tcttgcttag agataccacc tttcccctga agtgttcctt ccatgtttta 8820

cggcgagatg gtttctcctc gcctggccac tcagccttag ttgtctctgt tgtcttatag 8880

aggtctactt gaagaaggaa aaacaggggg catggtttga ctgtcctgtg agcccttctt 8940

ccctgcctcc cccactcaca gtgacccgga atccctcgac atggcagtct agcactagtg 9000

cggccgcaga tctgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 9060

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 9120

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa a 9171

<210> 20

<211> 22

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 20

ctgttgtgtg actctggtaa ct 22

<210> 21

<211> 22

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 21

aaatctctag cagtggcgcc cg 22

<210> 22

<211> 20

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 22

ttcgctttca agtccctgtt 20

<210> 23

<211> 22

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 23

gctgtcatct cttgtgggct gt 22

<210> 24

<211> 26

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 24

cctgtcatgc ccacacaaat ctctcc 26

<210> 25

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 25

actcatggga gctgctggtt c 21

<210> 26

<211> 55

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 26

gtccactcat tcttggatcc accggtgcca ccatgcaaat agagctctcc acctg 55

<210> 27

<211> 52

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 27

cgtttcaaga ctggtgggtt ttggctaggg tgtcttgaat tctgggagaa gc 52

<210> 28

<211> 52

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 28

gcttctccca gaattcaaga caccctagcc aaaacccacc agtcttgaaa cg 52

<210> 29

<211> 59

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 29

tccagaggtt gattgtcgac gtttaaacgc ggccgctcag tagaggtcct gtgcctcgc 59

<210> 30

<211> 56

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 30

acaaaaattc aaaattttat cgataagagc atgcgtgagg ctccggtgcc cgtcag 56

<210> 31

<211> 51

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 31

catggtggca ccggtggatc caagaatgag tcacgacacc tgaaatggaa g 51

Claims (11)

1. The codon-optimized coagulation factor VIII gene is characterized in that the nucleotide sequence of the codon-optimized coagulation factor VIII gene is SEQ ID NO 8 or SEQ ID NO 10.

2. A nucleic acid construct comprising the codon optimized factor VIII gene of claim 1.

3. The nucleic acid construct of claim 2, wherein the nucleic acid construct is a non-viral vector or a viral vector.

4. The nucleic acid construct of claim 3, wherein the viral vector is a lentiviral vector or an adeno-associated viral vector, and the codon-optimized factor VIII gene is located in the expression frame of the lentiviral vector or adeno-associated viral vector.

5. A lentivirus virally packaged from the nucleic acid construct of claim 2.

6. A lentiviral vector system, wherein the lentiviral vector system comprises a helper plasmid and the nucleic acid construct of claim 2.

7. The lentiviral vector system of claim 6, further comprising a host cell.

8. A composition for preventing or treating a coagulation factor deficiency disease, wherein the effective substances of the composition comprise one or more of the following substances: the codon-optimized factor VIII gene of claim 1; the nucleic acid construct of claim 2; the lentivirus of claim 5.

9. A cell line infected with the lentivirus of claim 5.

10. Use of the codon optimized factor VIII gene according to claim 1, the nucleic acid construct according to claim 2, the lentivirus according to claim 5, the composition according to claim 8 or the cell line according to claim 9 for the preparation of a medicament for the prevention and/or treatment of a factor deficiency disorder.

11. The use of claim 10, wherein the coagulation factor deficiency disease comprises one or more of hemophilia a, hemophilia B, and hemophilia C.

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