CN111803617A - Microcapsule-loaded stem cell system and preparation method thereof - Google Patents

Microcapsule-loaded stem cell system and preparation method thereof Download PDF

Info

Publication number
CN111803617A
CN111803617A CN202010685869.XA CN202010685869A CN111803617A CN 111803617 A CN111803617 A CN 111803617A CN 202010685869 A CN202010685869 A CN 202010685869A CN 111803617 A CN111803617 A CN 111803617A
Authority
CN
China
Prior art keywords
stem cells
stem cell
microcapsules
cell system
incubation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010685869.XA
Other languages
Chinese (zh)
Inventor
朱瑜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kadulan Guangzhou Life Gene Engineering Technology Co ltd
Original Assignee
Kadulan Guangzhou Life Gene Engineering Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kadulan Guangzhou Life Gene Engineering Technology Co ltd filed Critical Kadulan Guangzhou Life Gene Engineering Technology Co ltd
Priority to CN202010685869.XA priority Critical patent/CN111803617A/en
Publication of CN111803617A publication Critical patent/CN111803617A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5005—Wall or coating material
    • A61K9/5063—Compounds of unknown constitution, e.g. material from plants or animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system

Abstract

The invention discloses a stem cell system internally loaded with microcapsules, which takes stem cells as a cell carrier to load the microcapsules, wherein the loading mode is a cell endocytosis method; wherein the microcapsule contains polypeptide substance. The method has the advantages of simple and stable operation, deepening essence, gradual process and safety guarantee.

Description

Microcapsule-loaded stem cell system and preparation method thereof
Technical Field
The invention belongs to the technical field of biomedicine and nanomedicine, and particularly relates to a stem cell system internally loaded with microcapsules and a preparation method thereof.
Background
Neural stem cells (neural stem cells) refer to a cell population that exists in the nervous system, has the potential to differentiate into neurons, astrocytes and oligodendrocytes, and thus is capable of generating a large amount of brain cell tissue, and is capable of self-renewal, and sufficiently providing a large amount of brain tissue cells.
Is a type of mother cell with division potential and self-renewal ability, which can generate various types of cells of nervous tissues by unequal division modes. It is emphasized that different neural stem cell types produce different types and distributions of daughter cells in all neural tissues such as the cerebrospinal fluid.
The active peptide mainly controls the growth, development, immunoregulation and metabolism of a human body, the active peptide is in a balanced state in the human body, if the active peptide is reduced, the function of the human body is changed significantly, for children, the growth and development of the children become slow, even stop, a dwarfism is formed after a long time, for adults or old people, after the active peptide is lacked, the immunity of the children is reduced, the metabolism is disordered, the endocrine disorder is caused, and various diseases such as insomnia, emaciation or edema are caused. Because the active peptide also acts on the nervous system, the human body can become sluggish in action, and the head and the brain can not be clever any more, and more importantly, the active peptide is reduced, so that all parts of the human body can be gradually aged, and various diseases can be caused.
Disclosure of Invention
The invention mainly aims to overcome the defects of the prior art and provide a stem cell system internally loaded with microcapsules and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a stem cell system internally loaded with microcapsules takes stem cells as a cell carrier, and the stem cells are loaded in a way of endocytosis; wherein the microcapsule contains polypeptide substance.
Preferably, the stem cell is a mesenchymal stem cell or a neural stem cell.
Preferably, an endocytosis method is used, comprising the steps of: CO-incubating the stem cells and the microcapsules in a stem cell culture solution, wherein the number of the stem cells is 105-108, the incubation time is 1-96 h, the drug concentration is 1-100 mu g/mL, the incubation temperature is 30-45 ℃, and other incubation conditions are that the stem cells and the microcapsules contain 1-10% of CO2。
Preferably, the incubation time is 12h, and the drug concentration is50 μ g/mL, incubation temperature 37 ℃ and other incubation conditions were 5% CO2。
The method has the advantages of simple and stable operation, deepening essence, gradual process and safety guarantee.
Detailed Description
The present invention will be further described below, and it should be noted that the following examples are provided to illustrate the detailed embodiments and specific procedures based on the technical solution, but the scope of the present invention is not limited to the examples.
The invention relates to a stem cell system internally loaded with microcapsules, which takes stem cells as a cell carrier and loads the microcapsules in a cell endocytosis method; wherein the microcapsule contains polypeptide substance.
Preferably, the stem cell is a mesenchymal stem cell or a neural stem cell.
Preferably, an endocytosis method is used, comprising the steps of: CO-incubating the stem cells and the microcapsules in a stem cell culture solution, wherein the number of the stem cells is 105-108, the incubation time is 1-96 h, the drug concentration is 1-100 mu g/mL, the incubation temperature is 30-45 ℃, and other incubation conditions are that the stem cells and the microcapsules contain 1-10% of CO2。
Preferably, the incubation time is 12h, the drug concentration is 50. mu.g/mL, the incubation temperature is 37 ℃, and other incubation conditions are 5% CO2。
Various corresponding changes and modifications can be made by those skilled in the art based on the above technical solutions and concepts, and all such changes and modifications should be included in the protection scope of the present invention.

Claims (4)

1. A stem cell system internally loaded with microcapsules is characterized in that stem cells are used as a cell carrier in the system, and the system is loaded with the microcapsules in a manner of endocytosis; wherein the microcapsule contains polypeptide substance.
2. The microencapsulated stem cell system according to claim 1 wherein the stem cells are mesenchymal stem cells or neural stem cells.
3. A method for preparing the microencapsulated stem cell system according to claim 1, wherein the endocytosis method is used, which comprises the steps of: CO-incubating the stem cells and the microcapsules in a stem cell culture solution, wherein the number of the stem cells is 105-108, the incubation time is 1-96 h, the drug concentration is 1-100 mu g/mL, the incubation temperature is 30-45 ℃, and other incubation conditions are that the stem cells and the microcapsules contain 1-10% of CO2。
4. The method according to claim 7, wherein the number of stem cells is 106-107, the incubation time is 12h, the drug concentration is 50 μ g/mL, the incubation temperature is 37 ℃, and other incubation conditions are 5% CO2。
CN202010685869.XA 2020-07-16 2020-07-16 Microcapsule-loaded stem cell system and preparation method thereof Pending CN111803617A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010685869.XA CN111803617A (en) 2020-07-16 2020-07-16 Microcapsule-loaded stem cell system and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010685869.XA CN111803617A (en) 2020-07-16 2020-07-16 Microcapsule-loaded stem cell system and preparation method thereof

Publications (1)

Publication Number Publication Date
CN111803617A true CN111803617A (en) 2020-10-23

Family

ID=72866533

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010685869.XA Pending CN111803617A (en) 2020-07-16 2020-07-16 Microcapsule-loaded stem cell system and preparation method thereof

Country Status (1)

Country Link
CN (1) CN111803617A (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101489539A (en) * 2006-05-10 2009-07-22 生物相容英国有限公司 Spherical or non-spherical microcapsules comprising GLP-1 peptides, their production and use
CN104127884A (en) * 2014-07-14 2014-11-05 云南省第一人民医院 Rheumatoid arthritis treatment microcapsule and preparation method thereof
CN105457037A (en) * 2014-08-18 2016-04-06 复旦大学 Stem cell tumor targeting system with internal nano-prodrug and preparation method thereof
US20160137716A1 (en) * 2013-04-12 2016-05-19 Samir El Andaloussi Therapeutic delivery vesicles
CN109498541A (en) * 2018-12-12 2019-03-22 福建省海西细胞生物工程有限公司 A kind of intelligence improves the stem cell microcapsule composition of a variety of skin problems
WO2019246014A1 (en) * 2018-06-18 2019-12-26 North Carolina State University Stem cell biomimetic nanoparticle therapeutic agents and uses thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101489539A (en) * 2006-05-10 2009-07-22 生物相容英国有限公司 Spherical or non-spherical microcapsules comprising GLP-1 peptides, their production and use
US20160137716A1 (en) * 2013-04-12 2016-05-19 Samir El Andaloussi Therapeutic delivery vesicles
CN104127884A (en) * 2014-07-14 2014-11-05 云南省第一人民医院 Rheumatoid arthritis treatment microcapsule and preparation method thereof
CN105457037A (en) * 2014-08-18 2016-04-06 复旦大学 Stem cell tumor targeting system with internal nano-prodrug and preparation method thereof
WO2019246014A1 (en) * 2018-06-18 2019-12-26 North Carolina State University Stem cell biomimetic nanoparticle therapeutic agents and uses thereof
CN109498541A (en) * 2018-12-12 2019-03-22 福建省海西细胞生物工程有限公司 A kind of intelligence improves the stem cell microcapsule composition of a variety of skin problems

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MOONHWAN CHOI等: "Therapeutic Use of Stem Cell Transplantation for Cell Replacement or Cytoprotective Effect of Microvesicle Released from Mesenchymal Stem Cell", 《MOL. CELLS》 *
尤启冬等: "《2020国家执业药师职业资格考试指南 药学专业知识 1 第8版》", 31 March 2020, 中国医药科技出版社 *
顾晓彤等: "细胞膜修饰的纳米粒载药系统的研究进展", 《中国医药工业杂志》 *

Similar Documents

Publication Publication Date Title
Apple et al. Neurogenesis in the aging brain
Shihabuddin et al. Stem cell transplantation for neurometabolic and neurodegenerative diseases
Plaitakis et al. Abnormal glutamate metabolism in an adult-onset degenerative neurological disorder
Harms et al. Dendritic spine plasticity: looking beyond development
Knapp et al. Short-and long-term lithium administration: effects on the brain's serotonergic biosynthetic systems
Aebischer et al. Transplantation of microencapsulated bovine chromaffin cells reduces lesion-induced rotational asymmetry in rats
Rodrigues et al. Hypoxia enhances proliferation of mouse embryonic stem cell‐derived neural stem cells
Singer et al. Neurotrophic activity of brain extracts in forelimb regeneration of the urodele, Triturus
IL136833A0 (en) Neural precursor cells, method for the production and use thereof in neural defect therapy
CN108384755A (en) A method of efficiently, efficiently inductive pluripotent stem cells are to neural stem cell differentiating
Schousboe et al. Energy substrates to support glutamatergic and GABAergic synaptic function: role of glycogen, glucose and lactate
Ikemoto et al. Tyrosine hydroxylase and aromatic L-amino acid decarboxylase do not coexist in neurons in the human anterior cingulate cortex
Huang et al. Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis
CN111803617A (en) Microcapsule-loaded stem cell system and preparation method thereof
Warringa et al. Cellular location of glutamine synthetase and lactate dehydrogenase in oligodendrocyte‐enriched cultures from rat brain
Kim et al. Gene and cell replacement via neural stem cells
Viggiano et al. Inhibition of long‐term potentiation by CuZn superoxide dismutase injection in rat dentate gyrus: involvement of muscarinic M1 receptor
Buresh et al. Changes in the constant potential in brain structures in rats during focal ischemia and systemic hypoxia
CN102533653B (en) 5-HT nerve cells induced by MSCs and ASCs and microencapsulation preparation method and application thereof
Koelle et al. Effects of glycyl-L-glutamine in vitro on the molecular forms of acetylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat.
Johnson et al. Growth from regenerating goldfish retinal cultures in the absence of serum or hormonal supplements: tissue extract effects
Maurizi The function of dreams (REM sleep): Roles for the hippocampus, melatonin, monoamines, and vasotocin
Kumegawa et al. Precocious differentiation of chick embryo pancreas in vitro: Roles of prednisolone, insulin and L-thyroxine
CN110684735A (en) Culture medium for rapidly proliferating neural stem cells
US7323333B2 (en) Materials and methods for regulating process formation in cell culture

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination