CN111802320A - Tail clamping device for construction of depression animal model, expression image acquisition system, model construction method and application - Google Patents
Tail clamping device for construction of depression animal model, expression image acquisition system, model construction method and application Download PDFInfo
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Abstract
The invention relates to the technical field of biology, and particularly discloses a tail clamping device, an expression image acquisition system, a construction method and an application of a model, wherein the tail clamping device is applied to construction of a depression animal model.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a tail clamping device, an expression image acquisition system, a construction method and application of a model, wherein the tail clamping device, the expression image acquisition system and the expression image acquisition system are applied to construction of a depression animal model.
Background
Depression is the most common mood disorder disease, with over 3 billion depressed patients worldwide. At least 80 million patients with depression die by suicide each year. The incidence of depression is 12% -17% throughout a person's life. The present etiology of depression is unknown, clinical antidepressant drugs have low efficacy, slow effect taking, large side effects and high drug withdrawal recurrence rate, so that the elucidation of the pathogenesis of depression, the search of effective targets and the research of the fast, efficient and low-side-effect antidepressant become scientific problems to be solved urgently.
To solve this problem, animal models of human diseases have failed. The animal model of human diseases is an animal experimental object and material with human disease simulation performance established in medical science research, and is used for researching the occurrence, development rule and prevention and treatment measures of human diseases. The animal model can simulate the disease state of human depression, so the animal model is widely applied to the research of the pathogenesis of the depression and the research and development of new antidepressant drugs and plays an important role in the research of the depression. Animal models of depression are typically evaluated by surface, structural and predictive validity.
Surface effectiveness (face validity) is the similarity of phenomena, meaning that the emotional performance of animals in animal models should have similarity to the corresponding emotional performance of humans; structural validity (constraint validity) means that animal models should have similar causes and behavioral responses to human emotional disorders, as well as common biological mechanisms; the predictive validity mainly refers to the reactivity of the model to classical drugs with effective corresponding mood disorders, and can provide a powerful reference for clinical drug screening.
At present, there are four major models for depression animal models, namely stress model, surgery model, chemical drug induction model, and genotype change genetic model. The most common depression animal model at present is a chronic unpredictable mild stress model (mss), which is the only model meeting the requirements of surface effectiveness, structural effectiveness and high prediction effectiveness, has multiple stress factors and low stress intensity and high effectiveness, and allows experimental animals to experience 2 kinds of stimulation every day in 3-5 weeks, wherein the stimulation kinds include squirrel cage inclination, padding wetting, empty cage box, binding, tail clamping, day and night reversal, forced swimming, hot water swimming, ice water swimming, crowding, stroboflash, fasting and water prohibition and the like, and the stimulation of the same kind does not appear on two consecutive days, so that the experimental animals cannot predict the occurrence of the stimulation. This stress method subjects animals to mild stress for long periods of time, making it more realistic to simulate the "difficulties" that people encounter in their daily lives.
Mice are one of the most prominent model organisms in the field of contemporary biology. In the laboratory, the laboratory personnel often grab the mouse with bare hands, and there are the following problems in the bare-handed grabbing: firstly, in the grabbing process, the phenomenon that a rat scratches or bites an experimenter easily occurs; second, for some female experimenters, a strong psychological barrier to gripping the mouse by hand may be created. The CUMS model is used for constructing the depressed rats, the tail clamping stimulation is adopted mostly, experimenters often clamp the rat tail by using common pliers, but the end parts of the existing pliers are flat, and the force of each person is different, so that when the rat tail is clamped by using the common pliers, the rat tail can be damaged or even bleed, the rat tail is inflamed, the tail can be further deepened when the rat tail is subjected to the stimulation related to water such as swimming, and the like, and the tail necrosis can be caused when the rat tail is serious; secondly, when the tail clamping stimulation is carried out again, the tail clamping position and force of the pliers are greatly different due to the damage or necrosis of the tail, and further the molding effect is possibly not obvious. The CUMS model constructs the depressed mouse and can adopt and hang the tail amazing, and the device commonly used twines the rat tail with the sticky tape and hangs it fixedly, and the shortcoming of this way is that the mouse struggles and extremely easily struggles to take off the sticky tape and falls to the ground, and the experimenter will redo the tail experiment once more again, so not only extravagant manpower and time, also influenced the result of experiment to a certain extent, use this novel tail tweezers of pressing from both sides and can avoid the emergence of this phenomenon.
Emotional reactions are physiological reactions accompanying human or animal psychological activities (e.g., pain, fear, anger, etc.), and the animal presents with pain as a tight eye socket, a flat cheek, an attempt to escape from the left and right probe, etc. Studies have shown that changes in facial expression can be used as a means of assessing pain in mice, and the acquisition of facial expression in mice is the basis for subsequent assessment. The common method is to take a single picture or record a video with a camera, but the taken picture is easy to be judged by an inexperienced experimenter, so that the experimental result has errors.
Disclosure of Invention
In view of the above technical problems, an object of the present invention is to provide a tail clamping device for constructing an animal model of depression.
The technical scheme is as follows:
the utility model provides a be applied to tail clamping device in depression animal model constructs, includes the centre gripping main part, be provided with the clamping part that is used for centre gripping experimental animals tail in the centre gripping main part.
The clamping main body comprises a first clamp body and a second clamp body which are hinged in a crossed mode, and the clamping portion is a clamping hole which is formed by a first clamping piece at one end of the first clamp body and a second clamping piece at one end of the second clamp body in an occluded mode and used for clamping the tail of the experimental animal.
Further, the clamping hole is formed by oppositely arranged grooves which are respectively arranged on the first clamping piece and the second clamping piece.
Furthermore, a pressure sensor is arranged in the groove; and a display device integrated with a data processing chip is arranged on the first clamp body, and the pressure sensor is connected with the data processing chip.
The other ends of the first forceps body and the second forceps body are respectively provided with a first handheld portion and a second handheld portion, and an adjusting mechanism capable of adjusting clamping force is arranged between the first handheld portion and the second handheld portion.
Further, the adjusting mechanism comprises a rack arranged on the inner side of the first handheld portion and a locking device arranged on the inner side of the second handheld portion, the locking device is provided with a rack cavity matched with the rack, and the rack cavity penetrates through the second handheld portion; and the inner wall of the rack cavity is provided with a locking assembly which is meshed with the rack and can lock the rack.
Furthermore, a ratchet wheel accommodating cavity and a pawl accommodating cavity are formed in the rack cavity, and the ratchet wheel accommodating cavity is respectively communicated with the pawl accommodating cavity and the rack cavity;
the locking assembly comprises a ratchet wheel arranged in the ratchet wheel accommodating cavity, and a pawl, an adjusting piece and an elastic element which are arranged in the pawl accommodating cavity; one end of the pawl is rotatably connected to the inner wall of the pawl accommodating cavity, and the other end of the pawl is meshed with the ratchet wheel; the adjusting piece comprises a cam block which is rotatably arranged in the pawl accommodating cavity and a toggle switch which is coaxially connected with the cam block and extends out of the locking device; one end of the elastic element is installed in the pawl accommodating cavity, and the other end of the elastic element is connected with the pawl.
The clamping main body comprises a belt-shaped body and a fixing part arranged at one end of the belt-shaped body, wherein the belt-shaped body is provided with clamping teeth, the fixing part is provided with a fastening hole, and stopping teeth matched with the clamping teeth are arranged in the fastening hole; the clamping part is a fastening ring which is formed when the strip-shaped body penetrates through the fastening hole and is used for clamping the tail of the experimental animal, and the stopping teeth and the clamping teeth are matched to lock the fastening ring.
Taking a laboratory mouse as an example, in a tail clamping stimulation experiment, an experimenter often adopts a hemostatic forceps to take a mouse tail, but a clamping part of the hemostatic forceps is flat, and because the force of each person is different, when the hemostatic forceps are used for clamping the mouse tail, the flat clamping part and the stress surface of the mouse tail are always concentrated on the upper side surface and the lower side surface of the contact between the mouse tail and the flat clamping part, so that the stress of the flat clamping part and the contact surface of the mouse tail are very uneven, the mouse tail is possibly damaged and bleedings, the mouse tail is inflamed, and further the experimental results of experimental mice in the same batch are different; in addition, the mouse used for the experiment is generally a living body, and the phenomenon that the mouse tail escapes from the pliers is easy to occur, so that a more satisfactory result can be obtained only by repeated experiments, the labor and the time are wasted, and the experimental result is influenced to a certain extent.
Therefore, the selection of the device for clamping the tail of the mouse in the tail clamping stimulation experiment is particularly important; in the experiment, when the clamping device is selected to clamp the rat tail, the clamping part of the clamping device should disperse the rat tail stress surface as much as possible and enable the rat tail stress to be more uniform, so that the rat tail cannot be damaged.
The invention also aims to provide an expression image acquisition system applied to construction of a depression animal model.
The technical scheme is as follows:
an expression image acquisition system for constructing a depression animal model comprises a processing module, and an expression image acquisition device and an image display device which are respectively connected with the processing module; wherein,
the expression image acquisition device is used for acquiring animal expression images in real time and sending the animal expression images to the processing module; the processing module is used for comparing the collected animal expression image with the stored animal expression and sending a comparison result to the image display device; the image display device is used for displaying the comparison result.
Further, the processing module comprises an image analysis module, a data analysis module and a data storage module, wherein the image analysis module is used for analyzing the animal expression images acquired by the expression image acquisition device. Specifically, the expression image acquisition device comprises an expression image acquisition device and an image analysis module, wherein the data analysis module is used for classifying acquired data according to the information of the image analysis module; the data storage module is used for receiving the analysis result of the data analysis module and storing data.
Further, the image analysis module comprises an image recognition module and an expression analysis module, and the image recognition module is used for recognizing the shot expression image; the expression analysis module is used for analyzing the shot animal expression image and comparing the animal expression image with the stored animal expression.
The emotional response of the animal in distress is manifested by tight eye sockets, flattened cheeks, attempts by the left and right probes to escape, etc. Taking a laboratory mouse as an example, research shows that the change of facial expression can be used as a method for evaluating pain of the mouse, in a tail clamping stimulation experiment, after the tail of the laboratory mouse is clamped to generate pain, facial behavior units of the laboratory mouse are used for establishing pain expression grades of the laboratory mouse, and the degree of the facial behavior units increases along with the increase of clamping force and pain response. For example, when a mouse tail is held, as the holding force increases, the following may occur:
(1) the eye sockets are tight, and the change of the area of the eye sockets of the mouse face can be displayed in a non-presenting state, a moderate presenting state and an obvious presenting state;
(2) changes in beard, stiffening and angulation along the face, possible "bunching" of the beard, etc., may be manifested in the absence, moderate, and overt presentations.
The collection of the expression of pain present in the subjects is the basis for the subsequent evaluation of the experiment. Due to different clamping forces, the degree of the facial behavior units of the experimental object may not be obvious or similar, and thus, the experimental personnel may make a judgment wrong.
In the experiment, a common mode for collecting the animal expression is to use a camera to carry out single shooting or video recording, but the shot picture is easy to have a judgment error without comparison of animal pain expression scoring tables, so that the experimental result has an error.
The invention also aims to provide a construction method of the depression animal model.
The technical scheme is as follows:
the construction method of the depression animal model comprises the steps of clamping the tail of an experimental animal by the tail clamping device, and collecting the expression of the experimental animal clamped by the tail clamping device by the expression image collecting system.
The depression is a disease with a plurality of factors, the pathogenesis of the depression is lack of deep and definite understanding at present, the mechanism research of the depression and the development of a new anti-depression drug can not be separated from an effective animal model, the research of the antidepressant and the pharmacodynamic action mechanism thereof is enhanced, and the method has very important significance for the healthy survival of human beings. At present, the animal model of depression is most commonly used as a stress model, and various stimuli are given to animals to enable the animals to generate stress response and show the depression state.
In addition, the fourth purpose of the invention is to provide application of the depression animal model.
The technical scheme is as follows:
the application of the depression animal model in screening the anti-depression medicine is obtained according to the construction method.
The basic research of the etiology and mechanism of the disease needs to depend on an animal model, and the establishment of the animal model is comprehensively considered from various aspects such as the pathogenesis, symptom expression, pathophysiology, clinical curative effect and the like of the disease. That is, the causes of animal behavior modification during model construction, the various somatic and mental symptoms exhibited by animals, the pathophysiological basis for animal behavior modification, and the effectiveness of clinically efficacious antidepressants in animal models should be similar to those in the clinic. In antidepressant pharmacological research, the existing depression animal models with limited categories cannot meet the screening of all antidepressant traditional Chinese medicines, and the depression animal models used in part of research are not always correct, so that the true action mechanism of the medicines cannot be accurately reflected.
The invention has the beneficial effects that: the tail clamping device is simple in structure, the clamping part of the tail clamping device is provided with the groove, when the clamping part of the tail clamping device is occluded, a clamping hole is formed, so that the tail clamping device can be occluded with the tail of an experimental animal, the hole pattern of the clamping part of the tail clamping device disperses the stress surface of the tail of the experimental animal and enables the tail of the experimental animal to be stressed more uniformly, the conditions of tail injury and bleeding of the experimental animal are reduced, tail inflammation of the experimental animal is avoided, the experiment time and the experiment cost are saved, and the tail clamping device can be used for fixing the experimental animal more conveniently, so that an experimenter can transfer the experimental animal or can be used in other experiments conveniently; the invention also provides an expression image acquisition system for the depression animal model by adopting the clamping device, wherein the expression image acquisition system captures the pain expression image of the animal in the tail-clipped stimulation experiment in real time and compares the pain expression image with the stored pain expression of the animal to provide an effective basis for experimental data, so that the accuracy of analysis and comparison of subsequent experimental results is improved; meanwhile, the invention also constructs a depression animal model, and provides a research basis for exploring the pathogenesis of depression and screening different depression drugs.
Drawings
In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.
FIG. 1 is a schematic structural view of a tail clamping device according to the present invention;
FIG. 2 is a schematic cross-sectional view of the tail-clamping device of the present invention;
FIG. 3 is an enlarged view of a portion A of FIG. 2;
FIG. 4 is an enlarged view of a portion B of FIG. 2;
FIG. 5a is a schematic cross-sectional view of the ratchet wheel being rotated clockwise or counterclockwise when the pawl is pushed by the cam block;
FIG. 5b is a schematic diagram of the toggle switch position in the state of FIG. 5 a;
FIG. 6a is a cross-sectional view of the pawl being disengaged from the cam block and engaging the ratchet teeth of the ratchet wheel;
FIG. 6b is a schematic diagram of a toggle switch position in the state of FIG. 6 a;
FIG. 7 is a schematic structural view of another embodiment of the tail-clamping device of the present invention;
FIG. 8 is a schematic view of the band-shaped body of FIG. 7 engaged with the fastening portion;
FIG. 9 is a schematic structural diagram of an expression image acquisition system applied to the construction of a depression animal model.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example one
Referring to fig. 1, an embodiment of a tail clamping device applied to the construction of a depression animal model according to the present invention, specifically, a tail clamping device applied to the construction of a depression animal model, includes a clamping main body, and a clamping portion for clamping a tail of an experimental animal is disposed on the clamping main body.
Specifically, in this embodiment, the clamping main body includes a first clamp body 1 and a second clamp body 2 which are hinged in a crossed manner, the clamping portion is a clamping hole 3 which is formed by a first clamping piece 11 at one end of the first clamp body 1 and a second clamping piece 21 at one end of the second clamp body 2 in an engaged manner and is used for clamping the tail of the experimental animal, and the clamping hole 3 is formed by grooves which are respectively arranged on the first clamping piece 11 and the second clamping piece 21 and are oppositely arranged.
Grooves are respectively formed in the first clamping piece 11 and the second clamping piece 21 of the first clamp body 1 and the second clamp body 2, so that the grooves in the first clamping piece 11 and the second clamping piece 21 are meshed to form a clamping hole 3; when needs centre gripping laboratory animal tail, loosen first pincers body 1 and second pincers body 2, arrange the laboratory animal tail in the recess of first holder 11 or second holder 21, then make first pincers body 1 and the first holder 11 and the second holder 21 interlock of second pincers body 2, with the laboratory animal tail centre gripping in the centre gripping hole 3 that forms, it is more even to enable clamping device to the application of force of laboratory animal tail like this, thereby the laboratory animal tail damage has been reduced, the condition of hemorrhage, experimental time and experimental expense have been practiced thrift, and fixed laboratory animal tail that can also be more convenient, make things convenient for the experimenter to be used for the device to transport the animal or be used for hanging in the tail experiment.
In one embodiment, referring to FIG. 3, a pressure sensor 31 is mounted in the recess; the first clamp body 1 is provided with a display device 32 integrated with a data processing chip 321, and the pressure sensor 31 is connected with the data processing chip 321. Specifically, a containing cavity is formed in a groove in the first caliper body 1, the pressure sensor 31 is installed in the containing cavity, and meanwhile, a silica gel gasket is arranged on the surface of the pressure sensor 31 and is in fit with the surface of the groove in the first caliper body 1. The purpose of setting up the silica gel gasket is to protect pressure sensor, damage pressure sensor 31's surface when preventing the centre gripping object.
The second clamp body 2 is provided with a display device 32, and the display device 32 is integrated with a data processing chip 321; the pressure sensor 31 is electrically connected to the data processing chip 321 through a wire, and the data processing chip 321 is electrically connected to the display device 32, wherein the wire is disposed in the first caliper body 1, and two ends of the wire are respectively connected to the pressure sensor 31 and the display device 32. When holding the tail of the experimental animal, the holding force can be measured by the pressure sensor 31, and after being converted into a digital signal by the data processing chip 321, the digital signal is transmitted to the display device 32 for displaying. Make the experimenter can make things convenient for audio-visual observation this press from both sides tail device to the size of the clamping-force that experimental animal tail was applyed, do benefit to the influence of different experimental parameters of record to the experimental result.
Preferably, the groove in this embodiment is arcuate. Establish the arc recess of symmetry with the recess, form centre gripping hole 3 by the recess interlock and be exactly circular port or oval hole, like this, the outline of experiment animal tail just can be laminated more to the pass, and like this, centre gripping hole 3 will disperse the stress surface of animal tail and make experiment animal tail atress more even, just can not cause the damage to the animal tail. In this embodiment, the other ends of the first forceps body 1 and the second forceps body 2 are respectively provided with a first holding portion 12 and a second holding portion 22, and an adjusting mechanism 4 for adjusting the clamping force is provided between the first holding portion 12 and the second holding portion 22.
When the clamping device is used, the thumb of a user is placed on the first holding part 12 or the second holding part 22, the index finger and/or the middle finger is placed on the second holding part 22 or the first holding part 12, and then the finger applies force to enable the first clamping piece 11 and the groove on the second clamping piece 21 to be meshed to form the clamping hole 3; when needing the centre gripping experimental animals tail, loosen first handheld portion 12 and/or the handheld portion 22 of second, place the experimental animals tail in the recess of first holder 11 or second holder 21, then finger application of force makes first holder 11 and the interlock of second holder 21, with the centre gripping of experimental animals tail in the centre gripping hole 3 that forms, can be more even to the application of force of experimental animals tail like this to reduce the condition that the experimental animals tail damaged, bleed.
In an embodiment, the adjusting mechanism 4 includes a rack 41 disposed inside the first holding portion 12 and a locking device 42 disposed inside the second holding portion 22, respectively, the locking device 42 defines a rack cavity 421 matching with the rack 41; the inner wall of the rack chamber 421 is provided with a locking component which is engaged with the rack 41 and can lock the rack 41. A ratchet wheel accommodating cavity 422 and a pawl accommodating cavity 423 are formed in the rack cavity 421, and the ratchet wheel accommodating cavity 422 is respectively communicated with the pawl accommodating cavity 423 and the rack cavity 421;
wherein the locking assembly comprises a ratchet 424 disposed in a ratchet receiving cavity 422, and a pawl 425, an adjuster 426 and a resilient member 427 disposed in a pawl receiving cavity 423; one end of the pawl 425 is rotatably connected to the inner wall of the pawl accommodating chamber 423, and the other end is engaged with the ratchet 424; the adjusting member 426 includes a cam block 426a rotatably disposed in the pawl accommodating chamber 423, and a toggle switch 426b coaxially connected to the cam block 426a and extending out of the locking device 42; the elastic member 427 has one end installed in the pawl accommodating chamber 423 and the other end connected to the pawl 425.
The rack 41 is capable of engaging with the ratchet teeth of the ratchet 424 in the ratchet accommodating chamber 422, so that the ratchet 424 is rotatable in a clockwise direction or a counterclockwise direction in the ratchet accommodating chamber 422. Here, the clockwise direction or the counterclockwise direction means that, when the rack 41 moves toward the rack chamber 421 in the direction of fig. 4, the rack 41 will drive the ratchet 424 engaged therewith to rotate, so that the ratchet 424 rotates in the counterclockwise direction.
The tail clamping device in the embodiment can select whether to adopt a locking assembly according to the actual situation: specifically, when the locking assembly is not needed, the toggle switch 426b located at the rear side of fig. 5a is merely pulled counterclockwise to the position shown in fig. 5b, the toggle switch 426b will drive the cam block 426a to rotate, the protrusion of the cam block 426a will push the pawl 425 to rotate counterclockwise, so that the end of the pawl 425 engaged with the ratchet 424 is disengaged from the ratchet of the ratchet 424, and the pawl 425 will also compress the elastic element 427 located at one side thereof, so that the elastic element 427 is in the elastic compression state (as shown in fig. 5 a); since the pawl 425 is disengaged from the ratchet teeth of the ratchet wheel 424, the ratchet wheel 424 is in an unlocked state, and after the ratchet teeth are pushed by the rack 41, the ratchet wheel 424 can rotate counterclockwise or clockwise; when the first handle 12 and the second handle 22 are closed, the rack 41 inside the first handle 12 enters the rack cavity 421 of the locking device 42 inside the second handle 22, and since the ratchet 424 is not locked, the rack 41 is not restricted or fixed, so that the first handle 12 and the second handle 22 can be easily opened or closed.
When the locking assembly is required, the toggle switch 426b located at the opposite rear side of fig. 6a is moved to the position shown in fig. 6b, and the pawl 425 and the cam block 426a are located at the position shown in fig. 6 a; since the pawl 425 is not held by the cam block 426a, when the rack 41 enters the rack chamber 421 to engage with the ratchet teeth of the ratchet wheel 424 and drive the ratchet wheel 424 to rotate clockwise, the rotating ratchet wheel 424 will push the pawl 425 to rotate outwards (counterclockwise), so that one end of the pawl 425 disengages from the ratchet teeth of the ratchet wheel 424 and compresses the elastic element 427 disposed on one side of the pawl 425, so that the elastic element 427 is in an elastic compression state; however, if the ratchet 424 rotates counterclockwise (i.e. when the rack 41 moves toward the direction of disengaging the rack chamber 421, the rack 41 pushes the ratchet teeth of the ratchet 424 to drive the ratchet 424 to rotate clockwise), the ratchet teeth of the ratchet 424 will abut against the pawl 425 to assume a braking state, so that the ratchet teeth of the ratchet 424 cannot rotate clockwise, and thus the ratchet teeth of the ratchet 424 will assume a locked state with respect to the rack 41, and the rack 41 cannot move toward the direction of disengaging the rack chamber 421. In tail clamping experiments, when the tail clamping device is used for clamping the tail of an experimental animal, the tail of the animal is placed into the clamping hole 3, the first handheld part 12 and the second handheld part 22 are closed, the tail of the experimental animal is clamped by the clamping hole 3, at the same time, the rack 41 enters the rack chamber 421 of the locking device 42, and since the locking assembly is in the working state, the ratchet wheel 424 can only rotate clockwise, the rack 41 is engaged with the ratchet teeth of the ratchet 424 and then can only move towards the second handle 22, but cannot move away from the second hand-held portion 22, a locked state of the rack 41 is established, even if the holding part holds the tail of the experimental animal, since the rack 41 is locked in the rack chamber 421, the rack 41 cannot be separated from the rack chamber 421, since the holding hole 3 is filled with the tail of the experimental animal, the rack 41 cannot move further toward the second holding portion 22, therefore, the first forceps body 1 and the second forceps body 2 can keep a fixed state under the condition of no external force; after confirming that the tail clamping device clamps the stable tail of the experimental animal, because the tail clamping experiment needs to continuously clamp for 1-2min, the hands of the experimenter do not need to apply force to the first handheld part 12 and the second handheld part 22, and the tail clamping device can also clamp the tail of the animal, so that the labor is saved, the clamping force of the clamping hole 3 to the tail of the experimental animal is stable, the tail of the experimental animal is stressed more uniformly, and the damage and bleeding conditions of the tail of the experimental animal are reduced.
Since the first forceps body 1 and the second forceps body 2 are hinged together in a crossed manner, the movement tracks of the first hand-holding portion 12 at one end of the first forceps body 1 and the second hand-holding portion 22 at one end of the second forceps body 2 are arc-shaped, so that the rack 41 can be designed into the arc-shaped rack 41 in an adaptive manner, and since the rack cavity 421 of the locking device 42 is matched with the rack 41, the rack cavity 421 is also designed into the arc-shaped in an adaptive manner. The gear chamber 421 is designed to penetrate the second handle 22, so as to ensure that the first handle 12 and the second handle 22 can be closed as much as possible after the gear 41 enters the gear chamber 421, so that the clamping force can be maximized.
Because the rack 41 that gets into in the rack chamber 421 can be by the locking subassembly locking (make rack 41 can not remove towards the direction of keeping away from the second handheld portion), make the tail clamping device be in the state of being locked, the experimenter just can demand use tail clamping device to exert different clamping-force to the experimental animal tail, and the clamping-force sensor 31 and the clamping-force display device 32 of cooperation centre gripping hole just can realize adjusting the clamping-force degree at any time in the tail clamping experiment.
Example two
Referring to fig. 7, in another embodiment of the tail clamping device applied to the construction of the animal model with depression according to the present invention, in this embodiment, the clamping main body includes a belt-shaped body 5 and a fixing portion 6 disposed at one end of the belt-shaped body 5, wherein the belt-shaped body 5 is provided with a latch 51, the fixing portion 6 is provided with a fastening hole 61, and the fastening hole 61 is provided with a backstop tooth 62 engaged with the latch 51; the clamping part is a fastening ring which is formed when the belt-shaped body 5 penetrates through the fastening hole 61 and is used for clamping the tail of the experimental animal, and the backstop teeth 62 are matched with the latch teeth 51 to lock the fastening ring.
During the use, the one end upset that is kept away from fixed part 6 with banded body 5 passes fastening hole 61 of fixed part 6 and forms the zha quan that is used for centre gripping experimental animals tail, because latch 51 on the banded body and the cooperation locking of stopping tooth 62 in the fastening hole 61, can only drive the part that banded body 5 passed fastening hole 61 and continue to remove in the direction of keeping away from fixed part 6.
EXAMPLE III
Referring to fig. 9, the expression image acquisition system applied to the construction of the animal model for depression according to the present invention, specifically, the expression image acquisition system in this embodiment includes a processing module, and an expression image acquisition device and an image display device respectively connected to the processing module; wherein,
the expression image acquisition device is used for acquiring animal expression images in real time and sending the animal expression images to the processing module; the processing module is used for comparing the collected animal expression image with the stored animal expression and sending a comparison result to the image display device; the image display device is used for displaying the comparison result.
In this embodiment, the processing module comprises an image analysis module, a data analysis module and a data storage module, wherein the image analysis module is used for analyzing the animal expression images acquired by the expression image acquisition device; the data analysis module is used for classifying the acquired data according to the information of the image analysis module; the data storage module is used for receiving the analysis result of the data analysis module and storing data.
In this embodiment, the image analysis module includes an image recognition module and an expression analysis module, and the image recognition module is configured to recognize a captured expression image; the expression analysis module is used for analyzing the shot animal expression image and comparing the animal expression image with the stored animal expression.
Example four
A construction method of a depression animal model comprises the steps of clamping the tail of an experimental animal by a clamping device, and collecting the expression of the experimental animal clamped by the clamping device by an expression image collecting system.
EXAMPLE five
An application of a depression animal model in screening of an anti-depression drug adopts the depression animal model obtained by the construction method in the fourth embodiment.
The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (13)
1. A tail clamping device applied to construction of a depression animal model is characterized in that: including the centre gripping main part, be provided with the clamping part that is used for centre gripping experimental animals tail in the centre gripping main part.
2. The clip tail device of claim 1, wherein: the clamping main body comprises a first clamp body (1) and a second clamp body (2) which are hinged in a crossed mode, and the clamping portion is a clamping hole (3) which is formed by meshing a first clamping piece (11) at one end of the first clamp body (1) and a second clamping piece (21) at one end of the second clamp body (2) and used for clamping the tail of the experimental animal.
3. The clip tail device of claim 2, wherein: the clamping hole (3) is formed by grooves which are respectively arranged on the first clamping piece (11) and the second clamping piece (21) and are oppositely arranged.
4. The clip tail device of claim 3, wherein: a pressure sensor (31) is arranged in the groove; the first clamp body (2) is provided with a display device (32) integrated with a data processing chip (321), and the pressure sensor (31) is connected with the data processing chip (321).
5. The clip tail device of claim 4, wherein: the other ends of the first forceps body (1) and the second forceps body (2) are respectively provided with a first handheld portion (12) and a second handheld portion (22), and an adjusting mechanism (4) capable of adjusting clamping force is arranged between the first handheld portion (12) and the second handheld portion (22).
6. The clip tail device of claim 5, wherein: the adjusting mechanism (4) comprises a rack (41) and a locking device (42), the rack (41) and the locking device (42) are respectively arranged on the inner sides of the first handheld part (12) and the second handheld part (22), and a rack cavity (421) matched with the rack (41) is formed in the locking device (42); the inner wall of the rack cavity (421) is provided with a locking component which is meshed with the rack (41) and can lock the rack (41).
7. The clip tail device of claim 6, wherein: a ratchet wheel accommodating cavity (422) and a pawl accommodating cavity (423) are formed in the rack cavity (421), and the ratchet wheel accommodating cavity (422) is communicated with the pawl accommodating cavity (423) and the rack cavity (421) respectively;
wherein the locking assembly comprises a ratchet wheel (424) arranged in the ratchet wheel accommodating cavity (422), and a pawl (425), an adjusting piece (426) and an elastic element (427) which are arranged in the pawl accommodating cavity (423); one end of the pawl (425) is rotatably connected to the inner wall of the pawl accommodating cavity (423), and the other end of the pawl (425) is meshed with the ratchet wheel (424); the adjusting piece (426) comprises a cam block (426a) rotatably arranged in the pawl accommodating cavity (423) and a toggle switch (426b) which is coaxially connected with the cam block (426a) and extends out of the locking device (42); one end of the elastic element (427) is installed in the pawl accommodating cavity (423), and the other end is connected with the pawl (425).
8. The clip tail device of claim 1, wherein: the clamping main body comprises a belt-shaped body (5) and a fixing part (6) arranged at one end of the belt-shaped body (5), wherein the belt-shaped body (5) is provided with clamping teeth (51), the fixing part (6) is provided with a fastening hole (61), and the fastening hole (61) is internally provided with stopping teeth (62) matched with the clamping teeth (51); the clamping part is a fastening ring which is formed when the strip-shaped body (5) penetrates through the fastening hole (61) and is used for clamping the tail of the experimental animal, and the backstop teeth (62) and the latch teeth (51) are matched to lock the fastening ring.
9. An expression image acquisition system for acquiring the expression of the experimental animal held by the tail-clamping device of claim 1, characterized in that: the expression image acquisition system comprises a processing module, and an expression image acquisition device and an image display device which are respectively connected with the processing module; wherein,
the expression image acquisition device is used for acquiring animal expression images in real time and sending the animal expression images to the processing module; the processing module is used for comparing the collected animal expression image with the stored animal expression and sending a comparison result to the image display device; the image display device is used for displaying the comparison result.
10. The expression image acquisition system for the animal model of depression according to claim 9, wherein: the processing module comprises an image analysis module, a data analysis module and a data storage module, wherein the image analysis module is used for analyzing the animal expression images acquired by the expression image acquisition device; the data analysis module is used for classifying the acquired data according to the information of the image analysis module; the data storage module is used for receiving the analysis result of the data analysis module and storing data.
11. The expression image acquisition system for the animal model of depression according to claim 10, wherein: the image analysis module comprises an image recognition module and an expression analysis module, and the image recognition module is used for recognizing the shot expression image; the expression analysis module is used for analyzing the shot animal expression image and comparing the animal expression image with the stored animal expression.
12. A construction method of a depression animal model is characterized in that:
holding a tail of a laboratory animal by using the tail holding device of any one of claims 1 to 8, and acquiring the expression of the laboratory animal held by the tail holding device by using the expression image acquisition system of any one of claims 9 to 11.
13. The application of an animal model of depression in screening of anti-depression drugs is characterized in that: the depression animal model is obtained according to the construction method of the depression animal model of claim 12.
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| CN201910295736.9A CN111802320A (en) | 2019-04-12 | 2019-04-12 | Tail clamping device for construction of depression animal model, expression image acquisition system, model construction method and application |
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| CN201910295736.9A CN111802320A (en) | 2019-04-12 | 2019-04-12 | Tail clamping device for construction of depression animal model, expression image acquisition system, model construction method and application |
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Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0636617U (en) * | 1992-10-23 | 1994-05-17 | 武田薬品工業株式会社 | Small animal tail retention device for experiments |
| JPH0847502A (en) * | 1994-08-09 | 1996-02-20 | Tsumura & Co | Retainer |
| US20050119207A1 (en) * | 2000-05-12 | 2005-06-02 | The Johns Hopkins University | Inhibition of interaction of PSD93 and PSD95 with nNOS and NMDA receptors |
| US20080168951A1 (en) * | 2007-01-11 | 2008-07-17 | Starr Life Sciences Corp. | Small animal restraining device with physiologic sensor mount |
| CN102405878A (en) * | 2011-08-03 | 2012-04-11 | 浙江工业大学 | Method for building depression animal model |
| CN102599070A (en) * | 2012-03-14 | 2012-07-25 | 浙江中医药大学 | Bionic latex tail clamping device |
| CN203634258U (en) * | 2013-12-18 | 2014-06-11 | 重庆联佰博超医疗器械有限公司 | Self-destructive clamping self-locking mechanism and disposable circumcision suturing instrument |
| CN203749637U (en) * | 2014-03-18 | 2014-08-06 | 苏州大学 | Device for fixing tail of laboratory mouse for intravenous injection |
| CN104095614A (en) * | 2014-07-10 | 2014-10-15 | 中国人民解放军第四军医大学 | Rat balance based multifunctional pain threshold measuring apparatus and method |
| CN203935292U (en) * | 2014-06-25 | 2014-11-12 | 李晓峰 | The visual holder of experimental rat tail vein |
| CN104798728A (en) * | 2015-05-08 | 2015-07-29 | 南京中医药大学 | Creation method and applications of postpartum depression animal models |
| CN104865417A (en) * | 2015-05-19 | 2015-08-26 | 江苏大学 | Plant leaf electrical parameter testing electrode capable of measuring and controlling on-line clamping force |
| CN204863544U (en) * | 2015-07-13 | 2015-12-16 | 华东医院 | Portable mouse fixation clamp |
| CN105342718A (en) * | 2015-10-10 | 2016-02-24 | 重庆康乐制药有限公司 | Laboratory mouse holder |
| CN205623797U (en) * | 2016-05-10 | 2016-10-12 | 沈阳医学院 | Outstanding skin of outstanding tail of multi -functional toy is grabbed and is held ware |
| CN106417166A (en) * | 2016-09-26 | 2017-02-22 | 重庆医科大学 | Sprague-Dawley rat-based suicide relevant behavior animal model construction method |
| US9622842B1 (en) * | 2016-01-14 | 2017-04-18 | University Of South Florida | Rodent immobilization apparatus and method of use thereof |
| CN107280798A (en) * | 2017-07-24 | 2017-10-24 | 上海市第人民医院 | A kind of tail vein injection haemostatic clamp |
| CN107341688A (en) * | 2017-06-14 | 2017-11-10 | 北京万相融通科技股份有限公司 | The acquisition method and system of a kind of customer experience |
| CN107662577A (en) * | 2017-11-01 | 2018-02-06 | 南京工业职业技术学院 | A kind of light antijoyride device of automobile |
| CN107771740A (en) * | 2016-08-31 | 2018-03-09 | 中国科学院上海药物研究所 | A kind of preparation method of agitated depression animal model and its application |
| CN108960023A (en) * | 2017-09-19 | 2018-12-07 | 炬大科技有限公司 | A kind of portable Emotion identification device |
| KR20180138392A (en) * | 2017-06-21 | 2018-12-31 | 동아대학교 산학협력단 | Apparatus fixing the position and location of mouse for imaging acquisition with multiple modalities |
-
2019
- 2019-04-12 CN CN201910295736.9A patent/CN111802320A/en active Pending
Patent Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0636617U (en) * | 1992-10-23 | 1994-05-17 | 武田薬品工業株式会社 | Small animal tail retention device for experiments |
| JPH0847502A (en) * | 1994-08-09 | 1996-02-20 | Tsumura & Co | Retainer |
| US20050119207A1 (en) * | 2000-05-12 | 2005-06-02 | The Johns Hopkins University | Inhibition of interaction of PSD93 and PSD95 with nNOS and NMDA receptors |
| US20080168951A1 (en) * | 2007-01-11 | 2008-07-17 | Starr Life Sciences Corp. | Small animal restraining device with physiologic sensor mount |
| CN102405878A (en) * | 2011-08-03 | 2012-04-11 | 浙江工业大学 | Method for building depression animal model |
| CN102599070A (en) * | 2012-03-14 | 2012-07-25 | 浙江中医药大学 | Bionic latex tail clamping device |
| CN203634258U (en) * | 2013-12-18 | 2014-06-11 | 重庆联佰博超医疗器械有限公司 | Self-destructive clamping self-locking mechanism and disposable circumcision suturing instrument |
| CN203749637U (en) * | 2014-03-18 | 2014-08-06 | 苏州大学 | Device for fixing tail of laboratory mouse for intravenous injection |
| CN203935292U (en) * | 2014-06-25 | 2014-11-12 | 李晓峰 | The visual holder of experimental rat tail vein |
| CN104095614A (en) * | 2014-07-10 | 2014-10-15 | 中国人民解放军第四军医大学 | Rat balance based multifunctional pain threshold measuring apparatus and method |
| CN104798728A (en) * | 2015-05-08 | 2015-07-29 | 南京中医药大学 | Creation method and applications of postpartum depression animal models |
| CN104865417A (en) * | 2015-05-19 | 2015-08-26 | 江苏大学 | Plant leaf electrical parameter testing electrode capable of measuring and controlling on-line clamping force |
| CN204863544U (en) * | 2015-07-13 | 2015-12-16 | 华东医院 | Portable mouse fixation clamp |
| CN105342718A (en) * | 2015-10-10 | 2016-02-24 | 重庆康乐制药有限公司 | Laboratory mouse holder |
| US9622842B1 (en) * | 2016-01-14 | 2017-04-18 | University Of South Florida | Rodent immobilization apparatus and method of use thereof |
| CN205623797U (en) * | 2016-05-10 | 2016-10-12 | 沈阳医学院 | Outstanding skin of outstanding tail of multi -functional toy is grabbed and is held ware |
| CN107771740A (en) * | 2016-08-31 | 2018-03-09 | 中国科学院上海药物研究所 | A kind of preparation method of agitated depression animal model and its application |
| CN106417166A (en) * | 2016-09-26 | 2017-02-22 | 重庆医科大学 | Sprague-Dawley rat-based suicide relevant behavior animal model construction method |
| CN107341688A (en) * | 2017-06-14 | 2017-11-10 | 北京万相融通科技股份有限公司 | The acquisition method and system of a kind of customer experience |
| KR20180138392A (en) * | 2017-06-21 | 2018-12-31 | 동아대학교 산학협력단 | Apparatus fixing the position and location of mouse for imaging acquisition with multiple modalities |
| CN107280798A (en) * | 2017-07-24 | 2017-10-24 | 上海市第人民医院 | A kind of tail vein injection haemostatic clamp |
| CN108960023A (en) * | 2017-09-19 | 2018-12-07 | 炬大科技有限公司 | A kind of portable Emotion identification device |
| CN107662577A (en) * | 2017-11-01 | 2018-02-06 | 南京工业职业技术学院 | A kind of light antijoyride device of automobile |
Non-Patent Citations (2)
| Title |
|---|
| 朱立静等: "交泰丸在正常大鼠及抑郁大鼠体内的药代动力学比较研究", 《药学学报》, vol. 53, no. 3, 28 February 2018 (2018-02-28), pages 425 - 431 * |
| 洪灯;齐亚灵;张彦慧;钟南田;郑小桃;周雯;董战玲;: "简单易操作的大鼠CUMS抑郁模型的构建方法", 中国卫生产业, no. 24, 25 August 2011 (2011-08-25), pages 7 - 8 * |
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