CN1117974A - Method for extracting antibody from yolk - Google Patents
Method for extracting antibody from yolk Download PDFInfo
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- CN1117974A CN1117974A CN 95110458 CN95110458A CN1117974A CN 1117974 A CN1117974 A CN 1117974A CN 95110458 CN95110458 CN 95110458 CN 95110458 A CN95110458 A CN 95110458A CN 1117974 A CN1117974 A CN 1117974A
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- yolk
- antibody
- extracting
- diluent
- liquid
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Abstract
The present invention provides a new method for extracting antibody from yolk. The high-immunity yolk is diluted with diluents of phosphate buffer solution, etc. (yolk:diluent=1:3-6), then added with 0.3-1.2% of formaldehyde solution (containing 37% of formal-dehyde), and then uniformly mixed and frozen at the temp. of 10 deg.C below for 4-10 h., then quickly defrozen, solids are removed and its liquid is collected, and said liquid is the invented extract richly containing antibody. The invented process is simple and practicable, the antibody is high in both yield and purity, strong in activity, easy to be assimilated and has the good effect for preventing and curing diseases.
Description
The invention belongs to the extracting method of antibody from yolk.
Yolk can enrichment the antibody that produced of body, wherein antibody concentration is close or slightly high in the concentration of antibody and the serum, and yolk obtains than serum is easy, therefore often adopts the yolk that contains antibody to carry out artificial immunity and inoculates.But owing to contain lipid such as a large amount of Yelkin TTS in the yolk, increased the viscosity of yolk greatly, can yolk lipid and water soluble protein are separated be more effectively utilize the wherein key of antibody.In recent years, research in this respect mainly contains PEG extraction method (Polson et al., 1985), xanthan gum extraction method (Hajime HATTA et al., 1990), ethanol supercritical CO
2Degreasing method (eight field first-class, 1994) etc., though on yield and purity better, be difficult to solve that reagent dosage is big, toxicity big, complex operation and need a difficult problem such as high speed centrifugation, therefore fail to apply aborning.
The present invention is to by the innovation of extracting the method for antibody in the yolk, is intended to simplify the extraction procedure of yolk antibody, improves the rate of recovery, keeps higher activity, reduces cost, and has certain preservative activity.
Main points of the present invention are, to antigens such as laying hen immunization infectious bursal disease viruses, after 2～3 immunizations, will contain the yolk of high-titer antibody, with dilution (yolk: diluent=1: 3-6) such as phosphate buffered saline buffers (PBS), ratio in 0.3-1.2% adds formaldehyde solution (containing formaldehyde 37%), mixing places-10 ℃ of following temperature to freeze through 4-10 hours, takes out quick-thawing, it is gelatin that yolk is, and a small amount of limpid liquid can occur.With yolk extruding, can have liquid to flow out, and with recrement with diluent (original volume 1/4) rinsing, collect liquid twice, be prepared antibody-solutions, frozen.
Extraction process flow process of the present invention is as follows:
The adding proportion of each composition of the present invention is as follows:
1 part in yolk
3-6 parts of diluents
Formaldehyde solution 0.3-1.2% (accounting for yolk+diluent sum)
Preparation process need not special or valuable instrument, and the amount of used formaldehyde does not seldom need other pharmaceutical chemicalss yet, therefore, realizes that expense of the present invention is very low.The antibody from yolk that obtains is active high, is injected in animal body and easily absorbs, and side effect is little, and protection is strong, easily preserves.The present invention has overcome the weak point of yolk antibody extraction method in the past, has great practical value.
Embodiment:
With 1 part of high-immunity yolk liquid, add 3 parts of 0.01M pH7.4 phosphate buffered saline buffers, mixing adds 0.5% formaldehyde solution (formaldehyde content is 37%) again, and mixing is put under-10 ℃ of conditions and is frozen, and thaws, and removes the yolk solid substance, is the extract that is rich in antibody.
Claims (3)
1. a novel method of extracting antibody from yolk is characterized in that using formaldehyde solution and yolk, diluent phosphate buffered saline buffer mixing, is placed under the low temperature and freezes, and quick-thawing extracts then, is the extract that is rich in antibody.
2. a kind of novel method of extracting antibody from yolk according to claim 1, it is characterized in that concentration be 37% formaldehyde solution account for dilution yolk liquid total amount 0.3-1.2%.
3. a kind of novel method of extracting antibody from yolk according to claim 1, the adding proportion that it is characterized in that yolk and diluent is 1: 3-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95110458 CN1117974A (en) | 1995-05-19 | 1995-05-19 | Method for extracting antibody from yolk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95110458 CN1117974A (en) | 1995-05-19 | 1995-05-19 | Method for extracting antibody from yolk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1117974A true CN1117974A (en) | 1996-03-06 |
Family
ID=5077838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95110458 Pending CN1117974A (en) | 1995-05-19 | 1995-05-19 | Method for extracting antibody from yolk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1117974A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066154C (en) * | 1998-05-21 | 2001-05-23 | 山东农业大学 | Process for preparing refined vitelline antibody |
| CN1325516C (en) * | 2005-10-20 | 2007-07-11 | 上海交通大学 | Biomimetic affinity purification method of vitellus immune globulin |
-
1995
- 1995-05-19 CN CN 95110458 patent/CN1117974A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066154C (en) * | 1998-05-21 | 2001-05-23 | 山东农业大学 | Process for preparing refined vitelline antibody |
| CN1325516C (en) * | 2005-10-20 | 2007-07-11 | 上海交通大学 | Biomimetic affinity purification method of vitellus immune globulin |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |