CN111796094B - Reagent card and fluorescence immunoassay equipment - Google Patents
Reagent card and fluorescence immunoassay equipment Download PDFInfo
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- CN111796094B CN111796094B CN202010701944.7A CN202010701944A CN111796094B CN 111796094 B CN111796094 B CN 111796094B CN 202010701944 A CN202010701944 A CN 202010701944A CN 111796094 B CN111796094 B CN 111796094B
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
A reagent card and fluorescence immunoassay equipment, which relate to the technical field of medical appliances; the reagent card comprises a card body; the card body is provided with an information area; the card body is provided with a body upper surface and a body lower surface which are oppositely arranged along the height direction of the card body; the information area is provided with a high-value signal feedback part and a low-value signal feedback part which has different light reflection coefficients from the high-value signal feedback part, and at least one of the high-value signal feedback part and the low-value signal feedback part is higher or lower than the upper surface of the card body in the height direction of the card body; the high value signal feedback part and the low value signal feedback part characterize the information of the reagent card through the combination of different numbers and/or different positions. The fluorescence immunoassay device is suitable for a reagent card. The application aims to provide a reagent card and fluorescent immunoassay equipment, so that the accuracy of the equipment for reading the information of the reagent card is improved to a certain extent, and the cost of the reagent card is reduced.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a reagent card and fluorescence immunoassay equipment.
Background
In clinical diagnosis, the auxiliary diagnosis of diseases is increasingly carried out by utilizing fluorescent markers, and the principle is that a sample containing or not containing disease characteristic information is added into a reagent card containing a specific detection disease substance, and then the sample is combined with the specific substance which is marked by the fluorescent marker on the reagent card in advance, so that the aim of quantitatively or qualitatively auxiliary diagnosis of the diseases is fulfilled by identifying the fluorescent intensity.
In order to improve the recognition efficiency, fluorescent information is usually read by adopting fluorescent immunoassay equipment, and meanwhile, the fluorescent immunoassay equipment can also recognize the reagent card to acquire information of the disease type to be detected, manufacturers and the like, and the identification is carried out in the form of bar codes or two-dimensional codes on the market at present, on one hand, the bar codes or the two-dimensional codes are required to be additionally attached to the reagent card, so that the production efficiency of the reagent card is low, and the cost is high; on the other hand, the reagent card is inevitably scratched or even damaged in the packaging, transportation and use processes, especially in the use process, samples are likely to be sputtered on the code plane of the bar code or the two-dimensional code, and once the bar code or the two-dimensional code is damaged or dirty, the fluorescent immunoassay equipment cannot read the information of the reagent card or read the error information.
Disclosure of Invention
The application aims to provide a reagent card and fluorescent immunoassay equipment, so that the accuracy of the equipment for reading the information of the reagent card is improved to a certain extent, and the cost of the reagent card is reduced.
In order to achieve the above object, the present application provides the following technical solutions:
a reagent card comprising a card body; the card body is provided with an information area;
the card body is provided with a body upper surface and a body lower surface which are oppositely arranged along the height direction of the card body;
the information area is provided with a high-value signal feedback part and a low-value signal feedback part which has different light reflection coefficients from the high-value signal feedback part, and at least one of the high-value signal feedback part and the low-value signal feedback part is higher or lower than the upper surface of the card body in the height direction of the card body;
the high value signal feedback portion and the low value signal feedback portion characterize the information of the reagent card by combinations of different numbers and/or different positions.
In any of the above solutions, optionally, the information area includes one or more information subregions;
each of the information subregions includes the high value signal feedback portion and the low value signal feedback portion;
the high-value signal feedback parts of different information subareas are the same or different;
the low value signal feedback portions of different ones of the information subregions are the same or different.
In any of the above solutions, optionally, the high-value signal feedback portion is a through hole;
the low-value signal feedback part is a blind hole, or the low-value signal feedback part and the upper surface of the body are on the same plane.
In any of the above embodiments, optionally, the low value signal feedback portion is a frosted surface.
In any of the above solutions, optionally, the low value signal feedback portion is a through hole;
the high-value signal feedback part is a blind hole, or the high-value signal feedback part and the upper surface of the body are on the same plane.
In any of the above technical solutions, optionally, the high value signal feedback portion and the low value signal feedback portion are blind holes;
the hole bottom of the low-value signal feedback part is a frosted surface, and the roughness of the hole bottom of the low-value signal feedback part is larger than that of the hole bottom of the high-value signal feedback part; and/or the hole bottom of the high-value signal feedback part is provided with a reflecting material.
In any of the above solutions, optionally, when the high-value signal feedback portion or the low-value signal feedback portion is a through hole, the through hole is a groove extending to a side surface of the card body and penetrating through the card body;
and/or when the high-value signal feedback part or the low-value signal feedback part is a blind hole, the blind hole is a groove which extends to the side surface of the card body and does not penetrate through the card body.
In any of the above technical solutions, optionally, the reagent card includes a sample loading window and a detection window; the sample adding window and the detection window are both arranged on the card body.
A fluorescence immunoassay device suitable for a reagent card, comprising a bracket, a slideway, an optical component, a driving component and a card carrier for connecting the reagent card; the slideway, the optical assembly and the driving assembly are respectively connected with the bracket;
the card carrier is slidably arranged in the slide way;
the driving assembly is in driving connection with the card carrying frame so that the card carrying frame can slide along the slideway;
the optical component is located above the slideway, the card carrying frame and the top of the slideway are provided with collecting through grooves, and the optical component can collect information of the reagent card through the collecting through grooves.
In any of the above technical solutions, optionally, the card carrier includes a card carrying flat sheet, a vertical wall, and a transverse pressing sheet disposed opposite to the card carrying flat sheet;
the transverse pressing piece is arranged above the card carrying flat piece and is provided with the collecting through groove;
the vertical wall is connected with the card carrying flat piece and the transverse pressing piece;
and a reflecting layer is arranged on a part or all of the card carrying flat sheet, which is close to the inner surface of the transverse pressing sheet.
In any of the above solutions, optionally, the driving assembly is connected to the card carrier through a traction head in a driving manner; the traction head can slide along the slideway; one end of the card carrier is provided with a bulge, and the traction head is provided with a groove matched with the bulge;
and/or the slide way is provided with a sensing device for detecting the reagent in the card carrier to be clamped in place.
In any of the above solutions, optionally, the optical component includes a fluorescent scanning head and an identification scanning head;
the fluorescent scanning head comprises a light source, a grating, a first lens, a spectroscope, a second lens, an optical filter, a third lens and a photosensitive element;
the first light emitted by the light source can sequentially pass through the grating, the first lens and the spectroscope, and is reflected to the second lens through the spectroscope, and irradiates at a detection window of the reagent card; the sample in the detection window of the reagent card is excited and then emits second light, and the second light passes through the second lens, the spectroscope, the optical filter and the third lens in sequence and then reaches the photosensitive element;
the identification scanning head is a contact image sensor, a charge coupled sensing device or a camera.
The beneficial effects of the application are mainly as follows:
according to the reagent card and the fluorescence immunoassay equipment provided by the application, at least one of the high-value signal feedback part and the low-value signal feedback part is higher or lower than the upper surface of the card body in the height direction of the card body, and the light reflection coefficient of the high-value signal feedback part is different from that of the low-value signal feedback part, so that the information of the reagent card is represented by the high-value signal feedback part and the low-value signal feedback part, the identification rate is higher, the phenomenon that the information reading accuracy is influenced by damage and dirt of a bar code and a two-dimensional code can be effectively avoided, and the success rate and the accuracy of the equipment for reading the information of the reagent card can be effectively improved; the high-value signal feedback part and the low-value signal feedback part of the reagent card have simple structures, do not need to be attached independently compared with bar codes and two-dimensional codes, improve the production efficiency of the reagent card to a certain extent, and reduce the production cost of the reagent card.
In order to make the above objects, features and advantages of the present application more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a perspective view of a reagent card according to an embodiment of the present application;
FIG. 2 is a front view of a reagent card according to an embodiment of the present application;
fig. 3 to 5 are schematic diagrams of three structures of a reagent card according to an embodiment of the present application;
FIG. 6 is an enlarged view of a portion of a reagent card according to an embodiment of the present application;
FIG. 7 is a front view of another configuration of a reagent card according to an embodiment of the present application;
FIG. 8 is a partial cross-sectional view of the fluorescence immunoassay apparatus shown in FIG. 7 in the D-D direction;
FIG. 9 is a perspective view of a fluorescence immunoassay apparatus according to an embodiment of the present application;
FIG. 10 is an enlarged view of the region E of the fluorescence immunoassay apparatus shown in FIG. 9;
FIG. 11 is a cross-sectional view of the fluorescence immunoassay apparatus shown in FIG. 9;
fig. 12 is an optical path schematic diagram of a fluorescent scanning head of a fluorescent immunoassay device according to an embodiment of the present application.
Icon: 1-a bracket; 2-a slideway; 20-a sensing device; 3-card carrier; 30-loading card flat sheets; 31-bulge; 32-transversely tabletting; a 5-optical component; 50-fluorescent scanning head; 500-light source; 501-grating; 502-a first lens; 503-spectroscope; 504-a second lens; 505-filters; 506-a third lens; 507-photosensitive element; 51-identity recognition scanning head; 6-a traction head; 60-grooves; 7-a drive assembly;
4-a reagent card; 40-a sample application window; 41-a detection window; 42-information area; 422-a high value signal feedback section; 423-a low value signal feedback section; 43-card body.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. The components of the embodiments of the present application generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the application, as presented in the figures, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present application and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present application, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
Some embodiments of the present application are described in detail below with reference to the accompanying drawings. The following embodiments and features of the embodiments may be combined with each other without conflict.
Examples
Referring to fig. 1-8, the present embodiment provides a reagent card, and fig. 1 is a perspective view of the reagent card provided in the present embodiment; FIG. 2 is a front view of the reagent card according to the present embodiment; fig. 3 to 5 are schematic views of three structures of the reagent card according to the present embodiment; fig. 6 is a partial enlarged view of the reagent card according to the present embodiment. FIG. 7 is a front view showing another structure of the reagent card according to the present embodiment; fig. 8 is a partial sectional view of the fluorescent immunoassay device shown in fig. 7 in the D-D direction. Fig. 3-5 and 7 use dashed lines to divide the information sub-areas A, B and C.
The reagent card provided by the embodiment is used for detecting the substance with the disease specific information in vitro, and particularly for carrying out auxiliary diagnosis of the disease by using fluorescent markers. For example, the reagent card may employ a fluorescence immunoassay device to read fluorescence information of the reagent card.
Referring to fig. 1-8, the reagent card includes a card body 43; the card body 43 is provided with an information area 42. The information area 42 may be used to characterize specific information carried by a specific reagent card such as the type of disease to be tested, the manufacturer, etc.
Along the height direction of the card body 43, the card body 43 has a body upper surface and a body lower surface which are oppositely disposed; the card body 43 also has a side surface connecting the upper surface of the body and the lower surface of the body.
The information area 42 is provided with a high value signal feedback section 422 and a low value signal feedback section 423; the light reflection coefficient of the high value signal feedback portion 422 is different from that of the low value signal feedback portion 423, and at least one of the high value signal feedback portion 422 and the low value signal feedback portion 423 is higher or lower than the body upper surface in the height direction of the card body 43; so that the discrimination between the high value signal feedback part 422 and the low value signal feedback part 423 is obvious, and the success rate of the device reading information can be effectively improved. Wherein at least one of the high value signal feedback portion 422 and the low value signal feedback portion 423 is higher or lower than the body upper surface in the height direction of the card body 43, specifically, the high value signal feedback portion 422 is higher or lower than the body upper surface in the height direction of the card body 43, or the low value signal feedback portion 423 is higher or lower than the body upper surface in the height direction of the card body 43, or both the high value signal feedback portion 422 and the low value signal feedback portion 423 are not on the same plane as the body upper surface.
The high value signal feedback 422 and the low value signal feedback 423 characterize the information of the reagent card by a combination of different numbers and/or different positions. That is, the information of the reagent card is characterized by the combination of the high value signal feedback parts 422 and the low value signal feedback parts 423 in different numbers, or the information of the reagent card is characterized by the combination of the high value signal feedback parts 422 and the low value signal feedback parts 423 in different positions, or the information of the reagent card is characterized by the combination of the high value signal feedback parts 422 and the low value signal feedback parts 423 in different numbers and different positions. By adopting the combination of the number and/or the positions of the high value signal feedback part 422 and the low value signal feedback part 423 to characterize the reagent card information, the utilization and identification efficiency is high, and the success rate of reading the information by the equipment can be effectively improved. Referring to fig. 3 to 5, in combination with table 2 below, the reagent card according to the present embodiment characterizes the information of the reagent card by using a combination of the high value signal feedback part 422 and the low value signal feedback part 423. The combination of the high-value signal feedback units 422 and the low-value signal feedback units 423 may be a combination of different numbers of the high-value signal feedback units 422 and different numbers of the low-value signal feedback units 423, a combination of different numbers of the high-value signal feedback units 422 and different numbers of the low-value signal feedback units 423 at different positions, a combination of the same numbers of the high-value signal feedback units 422 and different numbers of the low-value signal feedback units 423 at different positions, a combination of different numbers of the high-value signal feedback units 422 and different numbers of the low-value signal feedback units 423 at different positions, a combination of the high-value signal feedback units 422 at different positions, a combination of the low-value signal feedback units 423 at different positions, a combination of the high-value signal feedback units 422 at different positions, and the low-value signal feedback units 423 at different positions, or the like.
In the reagent card of the present embodiment, at least one of the high-value signal feedback portion 422 and the low-value signal feedback portion 423 is higher or lower than the upper surface of the card body 43 in the height direction, and the light reflection coefficient of the high-value signal feedback portion 422 is different from the light reflection coefficient of the low-value signal feedback portion 423, so that the information of the reagent card is represented by the high-value signal feedback portion 422 and the low-value signal feedback portion 423, the identification rate is higher, the phenomenon that the information reading accuracy is affected by breakage and dirt of the bar code and the two-dimensional code can be effectively avoided, and the success rate and the accuracy of reading the reagent card information by the device can be effectively improved; the high-value signal feedback part 422 and the low-value signal feedback part 423 of the reagent card have simple structures, do not need to be attached independently compared with bar codes and two-dimensional codes, improve the production efficiency of the reagent card to a certain extent, and reduce the production cost of the reagent card.
Referring to fig. 1-5, in an alternative to this embodiment, the information area 42 includes one or more information subregions. Different information subregions may be used to display different information of the reagent card, for example, one information subregion displays, characterizes the disease type information to be tested, another information subregion displays, characterizes manufacturer information, etc.
Each information subregion comprises a high value signal feedback part 422 and a low value signal feedback part 423 to characterize the information of the information subregion by a combination of different numbers and/or different positions of high value signal feedback parts 422 and low value signal feedback parts 423. That is, the information of the information subregion is represented by the combination of the high value signal feedback parts 422 and the low value signal feedback parts 423 in different numbers, or the information of the information subregion is represented by the combination of the high value signal feedback parts 422 and the low value signal feedback parts 423 in different positions, or the information of the information subregion is represented by the combination of the high value signal feedback parts 422 and the low value signal feedback parts 423 in different numbers and different positions. By using the combination of the number and/or the positions of the high value signal feedback part 422 and the low value signal feedback part 423 to characterize the information of the information subregion, the recognition efficiency is high, and the success rate of the device reading information can be effectively improved.
The high value signal feedback sections 422 of the different information subregions are identical or different.
The low value signal feedback sections 423 of the different information subregions are identical or different.
In the reagent card according to the present embodiment, different information of the reagent card is corresponding to different combination states of the high value signal feedback part 422 and the low value signal feedback part 423 in each information subregion one by one. Fig. 3-5 show that the three information sub-areas A, B and C represent different information using different combinations of the high value signal feedback portion 422 and the low value signal feedback portion 423. As shown in table 1, the A, B and C information sub-areas are used to characterize the reagent card manufacturer identification code, disease type and/or name, and lot information, respectively.
TABLE 1
| Information subregion | Characterization information |
| A | Reagent card manufacturer identification code |
| B | Disease category and/or name |
| C | Batch information |
For example, the reagent card manufacturer identification code may be the address at which the manufacturer produced the reagent card, the disease type and/or name may be for diagnosing influenza a, for diagnosing hepatitis c virus, etc., and the lot information is the lot from which the lot of reagent cards was produced. Of course, other reagent card information may also be characterized, not limited to the above list, nor to 3 information subregions.
For example, table 2 shows information represented by the information sub-regions of fig. 3-5, respectively, 1 representing a high value signal, and 0 representing a low value signal.
TABLE 2
In an alternative scheme of this embodiment, the high-value signal feedback portion 422 and/or the low-value signal feedback portion 423 may adopt a through hole, a blind hole, or a mode that the through hole, the blind hole, or the blind hole and the upper surface of the card body 43 are on the same plane, so that the distinction degree between the high-value signal feedback portion 422 and the low-value signal feedback portion 423 is obvious, and further the success rate of reading information by the device can be effectively improved. The through hole may be replaced with a groove extending to the side of the card body 43 and penetrating the card body 43, and the blind hole may be replaced with a groove extending to the side of the card body 43 and not penetrating the card body 43. For example, when the high-value signal feedback portion 422 is a through hole, the high-value signal feedback portion 422 may be configured to extend to a side surface of the card body 43 and penetrate through a groove of the card body 43 along a height direction of the card body 43; when the high-value signal feedback portion 422 is a blind hole, the high-value signal feedback portion 422 may have a structure extending to the side surface of the card body 43, and no groove penetrating the card body 43 along the height direction of the card body 43; when the low-value signal feedback portion 423 is a through hole, the low-value signal feedback portion 423 may have a structure extending to the side surface of the card body 43 and penetrating through the groove of the card body 43 along the height direction of the card body 43; when the low-value signal feedback portion 423 is a blind hole, the low-value signal feedback portion 423 may have a structure extending to the side surface of the card body 43, and no groove penetrating the card body 43 along the height direction of the card body 43. Fig. 7 and 8 show that the high-value signal feedback portion 422 extends to the side of the card body 43, and penetrates through the groove of the card body 43 along the height direction of the card body 43, and the low-value signal feedback portion 423 is on the same plane as the upper surface of the card body 43.
In an alternative of the present embodiment, the high-value signal feedback part 422 is a through hole; the low-value signal feedback portion 423 is a blind hole, that is, the low-value signal feedback portion 423 is a non-through hole, and the phenomenon that the information reading accuracy is affected due to damage and dirt of the bar code and the two-dimensional code can be effectively avoided by adopting the through hole and the blind hole. As will be appreciated by those skilled in the art, the low value signal feedback portion 423 is a through hole; the high-value signal feedback part 422 is a blind hole, so that the high-value signal feedback part 422 and the low-value signal feedback part 423 can be distinguished, and the phenomenon that the information reading accuracy is affected due to damage and dirt of the bar code and the two-dimensional code can be effectively avoided.
In an alternative of the present embodiment, the high-value signal feedback part 422 is a through hole; the low value signal feedback portion 423 is on the same plane with the upper surface of the card body 43, that is, the low value signal feedback portion 423 is coplanar with the upper surface of the body, so that the distinction degree between the high value signal feedback portion 422 and the low value signal feedback portion 423 is obvious, and the success rate of reading information by the device can be effectively improved. As will be appreciated by those skilled in the art, the low value signal feedback portion 423 is a through hole; the high-value signal feedback part 422 and the upper surface of the card body 43 are on the same plane, so that the high-value signal feedback part 422 and the low-value signal feedback part 423 can be distinguished, and the phenomenon that the information reading accuracy is affected due to damage and dirt of the bar code and the two-dimensional code can be effectively avoided.
That is, in the height direction of the reagent card, the reagent card has a high recognition face and a low recognition face. The high recognition surface is a high value signal feedback part 422, and the low recognition surface is a low value signal feedback part 423; alternatively, the high recognition surface is the low value signal feedback portion 423, and the low recognition surface is the high value signal feedback portion 422.
Alternatively, the low value signal feedback portion 423 is a frosted surface. Optionally, the hole bottom of the low value signal feedback portion 423 is a frosted surface. Optionally, the hole bottom of the low value signal feedback portion 423 is frosted. The low-value signal feedback portion 423 is roughened to further increase the distinction between the high-value signal feedback portion 422 and the low-value signal feedback portion 423, so that the success rate and accuracy of the device for reading the reagent card information can be further improved.
Referring to fig. 6, in an alternative of the present embodiment, the high value signal feedback portion 422 and the low value signal feedback portion 423 are blind holes.
The hole bottom of the low-value signal feedback part 423 is a frosted surface, and the roughness of the hole bottom of the low-value signal feedback part 423 is greater than that of the hole bottom of the high-value signal feedback part 422; the distinction between the high value signal feedback part 422 and the low value signal feedback part 423 is further increased, so that the success rate and the accuracy of the device for reading the reagent card information can be further improved. Alternatively, the hole bottom of the high-value signal feedback part 422 is a light surface.
In order to further increase the distinction between the high value signal feedback part 422 and the low value signal feedback part 423, the hole bottom of the high value signal feedback part 422 is provided with a reflective material, so that the success rate and the accuracy of the device for reading the reagent card information can be further improved. Referring to fig. 1-5, in an alternative of this embodiment, the reagent card includes a loading window 40 and a detection window 41; the loading window 40 and the detection window 41 are both provided on the card body 43. The sample is placed through the loading window 40 and detected through the detection window 41.
Alternatively, the loading window 40, the detection window 41, and the information area 42 are disposed in this order along the card body 43.
Referring to fig. 9-12, the present embodiment provides a reagent card, and fig. 9 is a perspective view of the fluorescence immunoassay apparatus provided in the present embodiment with a housing removed; for more clear display of the structure, fig. 10 is an enlarged view of the region E of the fluorescent immunoassay device shown in fig. 9, and fig. 11 is a right side sectional view of the fluorescent immunoassay device shown in fig. 9; fig. 12 is a schematic diagram of an optical path of a fluorescent scanning head according to the present embodiment. Wherein the reagent card is not shown in fig. 10.
A fluorescence immunoassay device suitable for the reagent card. The fluorescence immunoassay device comprises a bracket 1, a slideway 2, an optical component 5, a driving component 7 and a card carrier 3 for connecting a reagent card 4; the slideway 2, the optical component 5 and the driving component 7 are respectively connected with the bracket 1; optionally, the slideway 2 is fixedly connected with the bracket 1; optionally, the optical assembly 5 is fixedly connected to the support 1.
The card carrier 3 is slidably arranged in the slide way 2; i.e. the card carrier 3 can move along the slide 2.
The driving assembly 7 is in driving connection with the card carrier 3 so that the card carrier 3 can slide along the slideway 2.
The optical component 5 is located above the slideway 2, the card carrier 3 and the top of the slideway 2 are provided with collecting through grooves, and the optical component 5 can collect information of the reagent card 4 located in the card carrier 3 through the collecting through grooves. The carrier 3 is connected by the driving assembly 7 in a driving way so that the carrier 3 with the reagent card 4 connected thereto can slide to a preset position so that the optical assembly 5 can collect information of the reagent card 4.
Optionally, the fluorescence immunoassay device comprises a housing and a control means. Part or all of the bracket 1 is fixedly arranged inside the shell. The control device is arranged inside the shell.
Referring to fig. 10, in an alternative of this embodiment, the card carrier 3 includes a card carrier plate 30, a vertical wall (not shown) and a lateral pressing plate 32 disposed opposite the card carrier plate 30.
The transverse pressing piece 32 is arranged above the card loading flat piece 30, and the transverse pressing piece 32 is provided with a collecting through groove.
The vertical wall connects the card-carrying flat sheet 30 and the transverse pressing sheet 32; the card holder 3 is detachably loaded with the reagent card 4 by the card holder flat plate 30, the vertical wall and the lateral pressing plate 32 to form a card slot for accommodating the reagent card 4. For example, the card loading flat piece 30 and the lateral pressing piece 32 restrict the displacement of the reagent card 4 in the up-down direction of the reagent card 4, and the oppositely disposed vertical walls restrict the displacement of the reagent card 4 in the left-right direction of the reagent card 4.
Optionally, the card-carrying flat sheet 30 is provided with a light-reflecting layer near part or all of the inner surface of the lateral presser sheet 32. Optionally, the light reflecting layer is a light reflecting coating or a light reflecting film, or other light reflecting structures. Optionally, the reflective coating is coated by adopting a reflective paint with acrylic resin as a base material or by adopting a chlorinated rubber coating. The specific manner of the material adopted for the reflective layer can be selected according to actual needs.
Referring to fig. 9-11, in an alternative of this embodiment, the driving assembly 7 is connected to the card carrier 3 through the traction head 6, and the traction head 6 can slide along the slideway 2; so that the driving component 7 drives the traction head 6 to move along the slideway 2, and drives the card carrier 3 to move along the slideway 2.
Alternatively, the drive assembly 7 is a motor, a hydraulic or pneumatic cylinder, or other drive structure. Optionally, the drive assembly 7 is drivingly connected to the traction head 6 by gears, belts, or the like.
Optionally, one end of the card carrier 3 is provided with a protrusion 31, and the traction head 6 is provided with a groove 60 matched with the protrusion 31; the traction head 6 is better driven by the protrusion 31 and the groove 60 to move the card carrier 3 along the slideway 2.
Optionally, the slide 2 is provided with sensing means 20 for detecting the presence of the reagent card 4 in the card holder 3. By means of the sensing means 20 to detect if the reagent card 4 is in place in the card holder 3. Optionally, the card carrier 3 is provided with in-place through holes corresponding to the sensing devices 20, so that the sensing devices 20 pass through the in-place through holes to detect whether the reagent cards 4 are in place.
Referring to fig. 12, an alternative to this embodiment is shown in which the optical assembly 5 includes a fluorescent scanning head 50 and an identification scanning head 51. Sample information of the reagent card 4 is detected by the fluorescent scanning head 50, and specific information such as the type of disease to be detected, the manufacturer, etc. of the reagent card 4 is identified by the identification scanning head 51.
Optionally, the fluorescent scanning head 50 includes a light source 500, a grating 501, a first lens 502, a beam splitter 503, a second lens 504, a filter 505, a third lens 506, and a photosensitive element 507; alternatively, light source 500 is a Light Emitting Diode (LED) or other light source. Alternatively, the light emitted by the light source 500 is typically near ultraviolet light or other light.
The first light emitted by the light source 500 can sequentially pass through the grating 501, the first lens 502 and the spectroscope 503, and is reflected to the second lens 504 by the spectroscope 503, and irradiates at the detection window 41 of the reagent card 4; the sample in the detection window 41 of the reagent card 4 emits a second light after being excited, and the second light passes through the second lens 504, the spectroscope 503, the optical filter 505 and the third lens 506 in sequence and then reaches the photosensitive element 507; optionally, after the first light reaches the beam splitter 503, the beam splitter 503 reflects the light with the predetermined wavelength to enter the second lens 504, and the first light irradiates the detection window 41 of the reagent card 4 after passing through the second lens 504, and the fluorescent substance marked sample in the detection window 41 emits the second light after being excited; optionally, the second light is fluorescence such as red light or near infrared light. The amount of the fluorescent marker of the second light can be measured by analyzing the intensity of the light received by the photosensitive element 507 and comparing the intensity with a pre-stored value, so as to realize qualitative or quantitative detection.
Alternatively, the identification scan head 51 is a contact image sensor, a charge coupled sensing device or a camera, or other structure.
Alternatively, the contact image sensor is a CMOS image sensor (english full name CMOS image sensor, abbreviated CIS). The CMOS image sensor has the advantages of compact arrangement of photosensitive units, direct collection of light information reflected by a scanned object, no need of a lens group, low cost and the like.
The fluorescence immunoassay device provided in this embodiment is applicable to the reagent card 4 described above, and the technical features of the reagent card 4 disclosed above are also applicable to the fluorescence immunoassay device, and the technical features of the reagent card 4 disclosed above are not described repeatedly. The fluorescence immunoassay apparatus of the present embodiment has the advantages of the reagent card 4 described above, and the advantages of the reagent card 4 disclosed above are not repeated here.
It should be understood that, when the through hole or the blind hole or the groove is compared with the upper surface of the card body in the height direction, if the through hole refers to the surface that can be seen by the lowermost end of the hole, the "see" refers to the information that can be identified by the optical component, for example, the inner surface of the card loading flat sheet; if blind, the lower end surface of the hole is referred to as a blind hole; if the surface of the groove penetrating the groove is the surface visible at the lowest end of the groove, the "visible" refers to the information which can be identified by the optical component, for example, the inner surface of the card-carrying flat sheet; if the groove is non-through, the lower end surface of the groove is referred to.
For a clearer understanding of the present embodiment, the operation principle of the fluorescence immunoassay apparatus will be briefly described:
firstly, starting equipment and self-checking;
inserting the reagent card 4 with finished sample application into the card carrier 3;
the sensing device 20 detects whether the reagent card 4 is inserted in place;
if not, sending out prompt information through an alarm;
if the card is in place, the control unit controls the driving assembly 7 to rotate for a preset time t1, at this time, the driving assembly 7 moves the reagent card 4 and the card carrier 3 to a preset position along the slideway 2 through the traction head 6, in the process, the identity recognition scanning head 51 starts scanning at the preset time t2, because the card carrier flat sheet 30 is provided with the reflecting material and the reagent card 4 is provided with the high-value signal feedback part 422 which is a through hole and the low-value signal feedback part 423 which is a non-through hole, when the through hole exists, light irradiates on the reflecting material, at this time, the identity recognition scanning head 51 receives the high-value signal feedback, when the through hole exists, the light cannot irradiate on the reflecting material, at this time, the identity recognition scanning head 51 receives the low-value signal feedback;
comparing the feedback signal of the identity recognition scanning head 51 with the pre-stored information of the control unit, thereby recognizing and acquiring the information of the reagent card;
the drive assembly 7 stops for a predetermined time t3 after the identification scanning head 51 finishes scanning so that the sample reacts with the reagent in the reagent card 4;
the driving assembly 7 rotates for a preset time t4, and the fluorescent scanning head 50 scans fluorescent signals of the detection window 41;
the driving assembly 7 rotates reversely for a preset time t5, the reagent card 4 exits the card carrier 3, and detection is completed.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (10)
1. A reagent card, characterized by comprising a card body; the card body is provided with an information area;
the card body is provided with a body upper surface and a body lower surface which are oppositely arranged along the height direction of the card body;
the information area is provided with a high-value signal feedback part and a low-value signal feedback part which has different light reflection coefficients from the high-value signal feedback part, and at least one of the high-value signal feedback part and the low-value signal feedback part is higher or lower than the upper surface of the card body in the height direction of the card body;
the high value signal feedback portion and the low value signal feedback portion characterize the information of the reagent card by combinations of different numbers and/or different positions.
2. The reagent card of claim 1, wherein the information area comprises one or more information subregions;
each of the information subregions includes the high value signal feedback portion and the low value signal feedback portion;
the high-value signal feedback parts of different information subareas are the same or different;
the low value signal feedback portions of different ones of the information subregions are the same or different.
3. The reagent card of claim 2, wherein the high value signal feedback part is a through hole;
the low-value signal feedback part is a blind hole, or the low-value signal feedback part and the upper surface of the body are on the same plane.
4. A reagent card according to claim 3, wherein the low value signal feedback portion is a frosted surface.
5. The reagent card of claim 2, wherein the low value signal feedback part is a through hole;
the high-value signal feedback part is a blind hole, or the high-value signal feedback part and the upper surface of the body are on the same plane.
6. The reagent card of claim 2, wherein the high value signal feedback portion and the low value signal feedback portion are blind holes;
the hole bottom of the low-value signal feedback part is a frosted surface, and the roughness of the hole bottom of the low-value signal feedback part is larger than that of the hole bottom of the high-value signal feedback part; and/or the hole bottom of the high-value signal feedback part is provided with a reflecting material.
7. The reagent card of any one of claims 3 to 6, wherein when the high value signal feedback portion or the low value signal feedback portion is a through hole, the through hole is a groove extending to a side surface of the card body and penetrating the card body;
and/or when the high-value signal feedback part or the low-value signal feedback part is a blind hole, the blind hole is a groove which extends to the side surface of the card body and does not penetrate through the card body.
8. The reagent card of any one of claims 1-6, comprising a loading window and a detection window; the sample adding window and the detection window are both arranged on the card body.
9. A fluorescence immunoassay apparatus adapted for use with a reagent card according to any one of claims 1-8, wherein the fluorescence immunoassay apparatus comprises a rack, a slide, an optical assembly, a drive assembly, and a carrier for connecting the reagent card; the slideway, the optical assembly and the driving assembly are respectively connected with the bracket;
the card carrier is slidably arranged in the slide way;
the driving assembly is in driving connection with the card carrying frame so that the card carrying frame can slide along the slideway;
the optical component is positioned above the slideway, the card carrier and the top of the slideway are respectively provided with a collection through groove, and the optical component can collect the information of the reagent card through the collection through grooves;
the card carrying frame comprises a card carrying flat sheet, a vertical wall and a transverse pressing sheet which is arranged opposite to the card carrying flat sheet;
the transverse pressing piece is arranged above the card carrying flat piece and is provided with the collecting through groove;
the vertical wall is connected with the card carrying flat piece and the transverse pressing piece;
and a reflecting layer is arranged on a part or all of the card carrying flat sheet, which is close to the inner surface of the transverse pressing sheet.
10. The fluorescence immunoassay apparatus according to claim 9, wherein the drive assembly is drivingly connected to the carrier by a traction head; the traction head can slide along the slideway; one end of the card carrier is provided with a bulge, and the traction head is provided with a groove matched with the bulge;
and/or the slide way is provided with a sensing device for detecting the reagent in the card carrier to be clamped in place.
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| CN202010701944.7A CN111796094B (en) | 2020-07-20 | 2020-07-20 | Reagent card and fluorescence immunoassay equipment |
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| CN113533710B (en) * | 2021-07-13 | 2023-10-03 | 珠海丽珠试剂股份有限公司 | Traction mechanism and in-vitro diagnosis and analysis equipment using same |
| CN113747702B (en) * | 2021-07-20 | 2023-07-28 | 青岛歌尔智能传感器有限公司 | Packaging structure carrying identification information |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005147739A (en) * | 2003-11-12 | 2005-06-09 | Konica Minolta Photo Imaging Inc | Card material surface inspection device |
| CN101526987A (en) * | 2008-03-06 | 2009-09-09 | 财团法人工业技术研究院 | Region recognition system using braille coded pattern |
| CN203929791U (en) * | 2014-05-08 | 2014-11-05 | 深圳市新产业生物医学工程股份有限公司 | A kind of sample rack induction system and biochemical immunity all-in-one thereof of sample rack RFID identification |
| CN205458709U (en) * | 2016-02-22 | 2016-08-17 | 广东腾飞基因科技有限公司 | Novel blood sampling card |
| CN208538158U (en) * | 2018-07-04 | 2019-02-22 | 中企信诚(北京)防伪技术有限公司 | A kind of scannable two-dimension code label |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090032590A1 (en) * | 2007-08-02 | 2009-02-05 | Hopkins Billy D | Location, orientation, product and color identification apparatus, system and method for the blind or visually impaired |
-
2020
- 2020-07-20 CN CN202010701944.7A patent/CN111796094B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005147739A (en) * | 2003-11-12 | 2005-06-09 | Konica Minolta Photo Imaging Inc | Card material surface inspection device |
| CN101526987A (en) * | 2008-03-06 | 2009-09-09 | 财团法人工业技术研究院 | Region recognition system using braille coded pattern |
| CN203929791U (en) * | 2014-05-08 | 2014-11-05 | 深圳市新产业生物医学工程股份有限公司 | A kind of sample rack induction system and biochemical immunity all-in-one thereof of sample rack RFID identification |
| CN205458709U (en) * | 2016-02-22 | 2016-08-17 | 广东腾飞基因科技有限公司 | Novel blood sampling card |
| CN208538158U (en) * | 2018-07-04 | 2019-02-22 | 中企信诚(北京)防伪技术有限公司 | A kind of scannable two-dimension code label |
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