CN111790002A - Preparation method of medical biogel hemostatic dressing - Google Patents
Preparation method of medical biogel hemostatic dressing Download PDFInfo
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- CN111790002A CN111790002A CN202010451608.1A CN202010451608A CN111790002A CN 111790002 A CN111790002 A CN 111790002A CN 202010451608 A CN202010451608 A CN 202010451608A CN 111790002 A CN111790002 A CN 111790002A
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- 230000002439 hemostatic effect Effects 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 229920001661 Chitosan Polymers 0.000 claims abstract description 98
- 238000002156 mixing Methods 0.000 claims abstract description 81
- 239000000243 solution Substances 0.000 claims abstract description 80
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 55
- 238000001914 filtration Methods 0.000 claims abstract description 33
- 229920002749 Bacterial cellulose Polymers 0.000 claims abstract description 24
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000005016 bacterial cellulose Substances 0.000 claims abstract description 24
- 229910001424 calcium ion Inorganic materials 0.000 claims abstract description 24
- 241000255789 Bombyx mori Species 0.000 claims abstract description 17
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 16
- 229920002401 polyacrylamide Polymers 0.000 claims abstract description 16
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 16
- 238000004108 freeze drying Methods 0.000 claims abstract description 15
- 108010022355 Fibroins Proteins 0.000 claims abstract description 14
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 11
- PLKATZNSTYDYJW-UHFFFAOYSA-N azane silver Chemical compound N.[Ag] PLKATZNSTYDYJW-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000011259 mixed solution Substances 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims description 38
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 36
- 239000006185 dispersion Substances 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 24
- 239000000126 substance Substances 0.000 claims description 21
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 11
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 10
- 238000007710 freezing Methods 0.000 claims description 8
- 230000008014 freezing Effects 0.000 claims description 8
- MPNXSZJPSVBLHP-UHFFFAOYSA-N 2-chloro-n-phenylpyridine-3-carboxamide Chemical compound ClC1=NC=CC=C1C(=O)NC1=CC=CC=C1 MPNXSZJPSVBLHP-UHFFFAOYSA-N 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 7
- CTKINSOISVBQLD-UHFFFAOYSA-N Glycidol Chemical compound OCC1CO1 CTKINSOISVBQLD-UHFFFAOYSA-N 0.000 claims description 7
- 230000001351 cycling effect Effects 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 6
- 230000000415 inactivating effect Effects 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 5
- 102000004142 Trypsin Human genes 0.000 claims description 5
- 108090000631 Trypsin Proteins 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 5
- 239000012588 trypsin Substances 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract description 21
- 239000000661 sodium alginate Substances 0.000 abstract description 21
- 235000010413 sodium alginate Nutrition 0.000 abstract description 21
- 229940005550 sodium alginate Drugs 0.000 abstract description 21
- 210000004369 blood Anatomy 0.000 abstract description 16
- 239000008280 blood Substances 0.000 abstract description 16
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 8
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 25
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 11
- 208000032843 Hemorrhage Diseases 0.000 description 9
- 208000034158 bleeding Diseases 0.000 description 9
- 230000000740 bleeding effect Effects 0.000 description 9
- 239000002994 raw material Substances 0.000 description 8
- 230000006835 compression Effects 0.000 description 7
- 238000007906 compression Methods 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000023555 blood coagulation Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 206010052428 Wound Diseases 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 230000023597 hemostasis Effects 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 3
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- 230000029663 wound healing Effects 0.000 description 3
- 229920002101 Chitin Polymers 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000001338 self-assembly Methods 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- 206010063746 Accidental death Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005253 cladding Methods 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000000850 deacetylating effect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 230000009982 effect on human Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000003805 procoagulant Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
- A61L2300/104—Silver, e.g. silver sulfadiazine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a preparation method of a medical biogel hemostatic dressing, and relates to the field of biomedicine. Firstly, preparing fibroin mixed solution by using silkworm cocoons and polyvinyl alcohol solution, then mixing bacterial cellulose and the fibroin mixed solution, adding polyacrylamide, and performing freeze-thaw cycle to prepare a gel blank; and treating the gel blank with a chitosan quaternary ammonium salt treatment solution, sequentially mixing with a silver ammonia solution containing sodium alginate and a calcium ion solution, filtering, and freeze-drying to obtain the medical biogel hemostatic dressing. The medical biogel hemostatic dressing prepared by the invention has excellent antibacterial performance, has better adsorption capacity to blood, can quickly coagulate the blood, and has excellent mechanical property.
Description
Technical Field
The invention relates to the field of biomedicine, in particular to a preparation method of a medical biogel hemostatic dressing.
Background
The medical science indicates that the patient can die within 6-20 minutes if effective emergency measures are not taken due to acute bleeding. Shock occurs when the blood loss of a human body exceeds 20 percent of the whole blood volume; if the blood loss exceeds 40 percent of the total blood volume, death is imminent. According to statistics, the number of traumatic deaths accounts for 10% of the total number of deaths, and the number of deaths caused by massive bleeding accounts for 30% -40%, so early bleeding control is the main method for reducing the mortality rate.
At present, after various artery and vein puncture and catheter extraction operations in hospitals, compression hemostasis is needed to be carried out on wounds. The most widely applied compression hemostasis mode in hospitals is that medical staff adopt gauze to perform compression hemostasis. Medical personnel superimpose gauze over the wound and then use bandages or straps to secure. Such a way of operation has drawbacks: firstly, the bandage or the bandage does not have extensibility and viscosity, and can not automatically give compression force, so that after the bandage or the bandage is fixed, the compression force can be lost if the bandage or the bandage is not tightly bound, and gauze can possibly move if a patient moves; secondly, the gauze has poor blood absorption performance, is easy to infect wounds and realizes the compression of the wounds only by the compression force given by bandages or bands.
Most of the recently developed hemostatic dressings use chitosan, a novel biomaterial, as the hemostatic material. The chitosan has biocompatibility and biodegradability, is rich in resources and easy to obtain, is applied to the hemostatic dressing, and has the functions of resisting bacteria, diminishing inflammation, stopping bleeding, easing pain, promoting wound healing and the like. In order to further enhance the performance of chitosan as a hemostatic dressing, chitosan and other substances are mixed in the prior art to prepare the hemostatic dressing.
Chitosan is a polymer obtained by deacetylating chitin, which is a natural polymer extracted from organisms and mainly exists in cell walls of crustaceans (shrimps and crabs), insect shells or fungi. Chitin is a linear high molecular polysaccharide polymer formed by beta-1, 4 bonding of glucosamine and N-acetylglucosamine. The chitosan has good biocompatibility, bioactivity, no cytotoxicity and biological decomposability with cells, so the chitosan can be applied to biological materials and medical materials. The chitosan has positive electricity in the acid solution, so that the chitosan can attract the blood platelets to aggregate, and further achieve the effect of quickly stopping bleeding.
At present, the dressing made of chitosan non-woven fabric is widely used in clinic, however, the chitosan dressing has the defect of poor stress, and after contacting blood, the chitosan dressing can rapidly form gel and break. Has good hemostatic effect on general bleeding, good biocompatibility and small side effect on human body during wound healing. However, when acute bleeding or surgical bleeding occurs, the hemostasis speed and amount still cannot meet the requirement of emergency treatment for massive and rapid bleeding.
Therefore, it is an important matter to invent a novel hemostatic dressing which can solve the defects of poor hemostatic effect and the like of the existing hemostatic dressing so as to meet the requirements of related industries.
Disclosure of Invention
The invention aims to provide a medical biogel hemostatic dressing and a preparation method thereof, and aims to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
the medical biogel hemostatic dressing is characterized by mainly comprising the following raw material components in parts by weight: 20-30 parts of silk, 6-12 parts of chitosan quaternary ammonium salt, 8-15 parts of bacterial cellulose, 2-4 parts of polyacrylamide and 5-8 parts of sodium alginate.
The medical biogel hemostatic dressing is characterized by further comprising the following raw material components in parts by weight: 12-18 parts of polyvinyl alcohol and 2-4 parts of nano silver.
Preferably, the chitosan quaternary ammonium salt is prepared by treating chitosan with glycidol trimethyl ammonium chloride.
As optimization, the medical biogel hemostatic dressing mainly comprises the following raw materials in parts by weight: 25 parts of silk, 10 parts of chitosan quaternary ammonium salt, 8 parts of bacterial cellulose, 3 parts of polyacrylamide, 8 parts of sodium alginate, 12 parts of polyvinyl alcohol and 4 parts of nano-silver.
As optimization, the preparation method of the medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) degumming and crushing silkworm cocoons to prepare refined silk, mixing the refined silk with a protease solution, inactivating enzyme at high temperature, adding a polyvinyl alcohol solution, and stirring and mixing to obtain a fibroin mixed solution;
(2) mixing bacterial cellulose and the fibroin mixed solution obtained in the step (1), adding polyacrylamide, stirring and mixing, and performing freeze-thaw circulation to obtain a gel blank;
(3) mixing the gel blank obtained in the step (2) with the chitosan quaternary ammonium salt treatment solution, filtering to obtain a pretreated gel blank, mixing the pretreated gel blank with a silver ammonia solution, adding sodium alginate, stirring and mixing, filtering, and freeze-drying to obtain gel;
(4) mixing the gel obtained in the step (3) with a calcium ion solution, filtering, freezing and drying to obtain the medical biogel hemostatic dressing;
(5) and (4) performing index analysis on the medical biogel hemostatic dressing obtained in the step (4).
As optimization, the preparation method of the medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) mixing silkworm cocoons with a sodium carbonate solution with the mass fraction of 10% according to the mass ratio of 1:8, stirring for reaction, filtering to obtain degummed silkworm cocoons, crushing the degummed silkworm cocoons in a crusher for 20-40 min to obtain refined silk, mixing the refined silk with a trypsin solution with the mass fraction of 3% according to the mass ratio of 1:12, stirring for reaction, inactivating enzyme at high temperature to obtain a fibroin dispersion liquid, and mixing the fibroin dispersion liquid with a polyvinyl alcohol solution with the mass fraction of 10% according to the mass ratio of 3: 1;
(2) mixing bacterial cellulose and the substance obtained in the step (1) according to a mass ratio of 1: 20-1: 25, adding polyacrylamide with a mass of 0.2-0.5 times that of the bacterial cellulose, stirring and mixing to obtain a mixed dispersion liquid, freezing the mixed dispersion liquid at-40 to-20 ℃ for 6 hours, then thawing at room temperature, and performing freeze-thaw cycling for 3 times to obtain a gel blank;
(3) mixing the substance obtained in the step (2) with a chitosan quaternary ammonium salt treatment solution according to a mass ratio of 1: 6-1: 8, standing for reaction for 3-4 h, filtering to obtain a pretreated gel blank, mixing the pretreated gel blank with a silver ammonia solution with a mass fraction of 10% according to a mass ratio of 1:10, adding sodium alginate with a mass of 0.1-0.5 times that of the pretreated gel blank, stirring for reaction, filtering, and freeze-drying;
(4) mixing the substance obtained in the step (3) with a calcium ion solution according to the mass ratio of 1:10, standing for reaction, filtering, and freeze-drying;
(5) and (4) carrying out index analysis on the substance obtained in the step (4).
Preferably, the chitosan quaternary ammonium salt treatment solution in the step (3) is prepared by mixing chitosan and an acetic acid solution with the mass fraction of 2% according to the mass ratio of 1:10, adjusting the pH value of the mixed solution of the chitosan and the acetic acid solution to 10, collecting precipitates to obtain pretreated chitosan, mixing the pretreated chitosan and isopropanol according to the mass ratio of 1:8, adding a glycidol trimethyl ammonium chloride solution with the mass fraction of 20% and the mass fraction of 3-6 times that of the pretreated chitosan, stirring for reaction, adding ethanol for precipitation, filtering, drying to obtain chitosan quaternary ammonium salt, mixing the chitosan quaternary ammonium salt with water according to the mass ratio of 1:20, adding citric acid with the mass of 0.1-0.3 time that of the chitosan quaternary ammonium salt and triethanolamine with the mass of 0.2-0.3 time that of the chitosan quaternary ammonium salt, and stirring and mixing to obtain the chitosan quaternary ammonium salt treatment solution.
Preferably, the calcium ion solution in the step (4) is any one of a calcium chloride solution with a mass fraction of 5% or a calcium nitrate solution with a mass fraction of 3%.
Compared with the prior art, the invention has the beneficial effects that:
silk and chitosan quaternary ammonium salt are added when the medical biogel hemostatic dressing is prepared; firstly, silk is added into the medical biogel hemostatic dressing, on one hand, the silk can be matched with added bacterial cellulose to be used as a framework of the medical biogel hemostatic dressing, thereby improving the mechanical property of the medical biogel hemostatic dressing, preventing the medical biogel hemostatic dressing from being broken due to blood sucking and coagulation promotion when in use, and improving the strippability of a product, on the other hand, the surface of the silk is degraded after the added silk is treated by protease, thereby improving the number of active groups on the surface of the silk, and can absorb sufficient calcium ions after being mixed with a calcium ion solution, and the calcium ions can promote blood coagulation, thereby improving the coagulation promotion performance of the product on blood when the medical biogel hemostatic dressing is in use, meanwhile, because the silk is protein fiber, the silk can be decomposed by protease in plasma in the use process of the product, and the wound healing does not improve amino acid, thereby improving the use effect of the product; secondly, add chitosan quaternary ammonium salt in medical biogel hemostatic dressing, chitosan quaternary ammonium salt can adsorb and the silk surface under the effect of electrostatic force, thereby improve the antibacterial property of product, after follow-up and the silver ammonia solution that contains sodium alginate mixes, sodium alginate can be under the effect of electrostatic force, carry out self-assembly on the silk surface, simultaneously, because sodium alginate and the group on silk surface have the reductibility, can reduce silver ammonia solution, form nanometer silver, therefore sodium alginate can be with the firm cladding of nanometer silver on the silk surface in self-assembly process, and then further improve the antibacterial property of product, moreover, because sodium alginate can carry out the crosslinking under calcium ion solution, thereby further improve calcium ion content in the product, and improve the fastness of product, and then improve the effect of procoagulant of product to blood.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to more clearly illustrate the method provided by the present invention, the following examples are provided to illustrate the method of testing the indexes of the medical bio-gel hemostatic dressing prepared in the following examples as follows:
sterilization property: the medical biogel hemostatic dressing obtained in each example and the comparative product are respectively placed in a staphylococcus aureus culture solution and an escherichia coli culture solution with the same concentration, and the sterilization rate after the medical biogel hemostatic dressing and the comparative product are placed for 2 hours is measured.
Blood coagulation property: the medical biogel hemostatic dressings obtained in each example and the comparative example were cut into pieces of 1cm × 1cm × 0.5cm, and placed in beakers containing 10mL of blood, respectively, and the time for adsorbing the whole blood and the time for clotting the blood were measured.
Example 1
A medical biological gel hemostatic dressing mainly comprises the following raw material components in parts by weight: 25 parts of silk, 10 parts of chitosan quaternary ammonium salt, 8 parts of bacterial cellulose, 3 parts of polyacrylamide, 8 parts of sodium alginate, 12 parts of polyvinyl alcohol and 4 parts of nano-silver.
A preparation method of a medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) mixing a silkworm cocoon and a 10% sodium carbonate solution according to a mass ratio of 1:8, stirring and reacting for 80min at a temperature of 60 ℃ and a rotating speed of 300r/min, filtering to obtain a degummed silkworm cocoon, crushing the degummed silkworm cocoon in a crusher for 40min to obtain refined silk, mixing the refined silk and a 3% trypsin solution according to a mass ratio of 1:12 in a beaker, stirring and reacting for 50min at a temperature of 36 ℃ and a rotating speed of 300r/min, heating the material in the beaker to 90 ℃, keeping the temperature for 30min, inactivating enzyme to obtain a fibroin dispersion liquid, and mixing the fibroin dispersion liquid and a 10% polyvinyl alcohol solution according to a mass ratio of 3: 1;
(2) mixing bacterial cellulose and the substance obtained in the step (1) according to a mass ratio of 1:20, adding polyacrylamide with the mass of 0.4 time that of the bacterial cellulose, stirring and mixing for 40min at a temperature of 35 ℃ and a rotating speed of 280r/min to obtain a mixed dispersion liquid, freezing the mixed dispersion liquid for 6h at a temperature of-30 ℃, then unfreezing for 6h at room temperature, and performing freeze-thaw cycling for 3 times to obtain a gel blank;
(3) mixing the gel blank obtained in the step (2) with a chitosan quaternary ammonium salt treatment solution according to a mass ratio of 1:8, standing and reacting for 4 hours at the temperature of 40 ℃, filtering to obtain a pretreated gel blank, mixing the pretreated gel blank with a silver ammonia solution with the mass fraction of 10% in a conical flask according to a mass ratio of 1:10, adding sodium alginate with the mass of 0.2 times that of the pretreated gel blank into the conical flask, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 180r/min, filtering, and freeze-drying;
(4) mixing the substance obtained in the step (3) with a calcium ion solution according to a mass ratio of 1:10, standing and reacting for 3 hours at the temperature of 35 ℃, filtering, and freeze-drying;
(5) and (4) carrying out index analysis on the substance obtained in the step (4).
Preferably, the chitosan quaternary ammonium salt treatment solution in the step (3) is prepared by mixing chitosan and an acetic acid solution with the mass fraction of 2% according to the mass ratio of 1:10, adjusting the pH value of the mixed solution of the chitosan and the acetic acid solution to 10, collecting precipitates to obtain pretreated chitosan, mixing the pretreated chitosan and isopropanol according to the mass ratio of 1:8, adding a glycidol trimethyl ammonium chloride solution with the mass fraction of 20% and the mass fraction of 4 times that of the pretreated chitosan, stirring for reaction, adding ethanol for precipitation, filtering to obtain filter residues, drying the filter residues at the temperature of 75 ℃ for 3 hours to obtain chitosan quaternary ammonium salt, mixing the chitosan quaternary ammonium salt and water according to the mass ratio of 1:20, adding citric acid with the mass of 0.2 time of that of the chitosan quaternary ammonium salt and triethanolamine with the mass of 0.3 time of the chitosan quaternary ammonium salt, and stirring and mixing to obtain the chitosan quaternary ammonium salt treatment solution.
Preferably, the calcium ion solution in the step (4) is a calcium chloride solution with the mass fraction of 5%.
Example 2
A medical biological gel hemostatic dressing mainly comprises the following raw material components in parts by weight: 25 parts of silk, 10 parts of chitosan quaternary ammonium salt, 8 parts of bacterial cellulose, 3 parts of polyacrylamide, 12 parts of polyvinyl alcohol and 4 parts of nano-silver.
A preparation method of a medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) mixing a silkworm cocoon and a 10% sodium carbonate solution according to a mass ratio of 1:8, stirring and reacting for 80min at a temperature of 60 ℃ and a rotating speed of 300r/min, filtering to obtain a degummed silkworm cocoon, crushing the degummed silkworm cocoon in a crusher for 40min to obtain refined silk, mixing the refined silk and a 3% trypsin solution according to a mass ratio of 1:12 in a beaker, stirring and reacting for 50min at a temperature of 36 ℃ and a rotating speed of 300r/min, heating the material in the beaker to 90 ℃, keeping the temperature for 30min, inactivating enzyme to obtain a fibroin dispersion liquid, and mixing the fibroin dispersion liquid and a 10% polyvinyl alcohol solution according to a mass ratio of 3: 1;
(2) mixing bacterial cellulose and the substance obtained in the step (1) according to a mass ratio of 1:20, adding polyacrylamide with the mass of 0.4 time that of the bacterial cellulose, stirring and mixing for 40min at a temperature of 35 ℃ and a rotating speed of 280r/min to obtain a mixed dispersion liquid, freezing the mixed dispersion liquid for 6h at a temperature of-30 ℃, then unfreezing for 6h at room temperature, and performing freeze-thaw cycling for 3 times to obtain a gel blank;
(3) mixing the gel blank obtained in the step (2) with a chitosan quaternary ammonium salt treatment solution according to a mass ratio of 1:8, standing and reacting for 4 hours at the temperature of 40 ℃, filtering to obtain a pretreated gel blank, mixing the pretreated gel blank and a silver ammonia solution with the mass fraction of 10% in a conical flask according to a mass ratio of 1:10, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 180r/min, filtering, and freeze-drying;
(4) mixing the substance obtained in the step (3) with a calcium ion solution according to a mass ratio of 1:10, standing and reacting for 3 hours at the temperature of 35 ℃, filtering, and freeze-drying;
(5) and (4) carrying out index analysis on the substance obtained in the step (4).
Preferably, the chitosan quaternary ammonium salt treatment solution in the step (3) is prepared by mixing chitosan and an acetic acid solution with the mass fraction of 2% according to the mass ratio of 1:10, adjusting the pH value of the mixed solution of the chitosan and the acetic acid solution to 10, collecting precipitates to obtain pretreated chitosan, mixing the pretreated chitosan and isopropanol according to the mass ratio of 1:8, adding a glycidol trimethyl ammonium chloride solution with the mass fraction of 20% and the mass fraction of 4 times that of the pretreated chitosan, stirring for reaction, adding ethanol for precipitation, filtering to obtain filter residues, drying the filter residues at the temperature of 75 ℃ for 3 hours to obtain chitosan quaternary ammonium salt, mixing the chitosan quaternary ammonium salt and water according to the mass ratio of 1:20, adding citric acid with the mass of 0.2 time of that of the chitosan quaternary ammonium salt and triethanolamine with the mass of 0.3 time of the chitosan quaternary ammonium salt, and stirring and mixing to obtain the chitosan quaternary ammonium salt treatment solution.
Preferably, the calcium ion solution in the step (4) is a calcium chloride solution with the mass fraction of 5%.
Example 3
A medical biological gel hemostatic dressing mainly comprises the following raw material components in parts by weight: 25 parts of silk, 8 parts of bacterial cellulose, 3 parts of polyacrylamide, 8 parts of sodium alginate, 12 parts of polyvinyl alcohol and 4 parts of nano-silver.
A preparation method of a medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) mixing a silkworm cocoon and a 10% sodium carbonate solution according to a mass ratio of 1:8, stirring and reacting for 80min at a temperature of 60 ℃ and a rotating speed of 300r/min, filtering to obtain a degummed silkworm cocoon, crushing the degummed silkworm cocoon in a crusher for 40min to obtain refined silk, mixing the refined silk and a 3% trypsin solution according to a mass ratio of 1:12 in a beaker, stirring and reacting for 50min at a temperature of 36 ℃ and a rotating speed of 300r/min, heating the material in the beaker to 90 ℃, keeping the temperature for 30min, inactivating enzyme to obtain a fibroin dispersion liquid, and mixing the fibroin dispersion liquid and a 10% polyvinyl alcohol solution according to a mass ratio of 3: 1;
(2) mixing bacterial cellulose and the substance obtained in the step (1) according to a mass ratio of 1:20, adding polyacrylamide with the mass of 0.4 time that of the bacterial cellulose, stirring and mixing for 40min at a temperature of 35 ℃ and a rotating speed of 280r/min to obtain a mixed dispersion liquid, freezing the mixed dispersion liquid for 6h at a temperature of-30 ℃, then unfreezing for 6h at room temperature, and performing freeze-thaw cycling for 3 times to obtain a gel blank;
(3) mixing the gel blank obtained in the step (2) and a silver ammonia solution with the mass fraction of 10% in a conical flask according to the mass ratio of 1:10, adding sodium alginate with the mass of 0.2 time that of the pretreated gel blank into the conical flask, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 180r/min, filtering, and freeze-drying;
(4) mixing the substance obtained in the step (3) with a calcium ion solution according to a mass ratio of 1:10, standing and reacting for 3 hours at the temperature of 35 ℃, filtering, and freeze-drying;
(5) and (4) carrying out index analysis on the substance obtained in the step (4).
Preferably, the chitosan quaternary ammonium salt treatment solution in the step (3) is prepared by mixing chitosan and an acetic acid solution with the mass fraction of 2% according to the mass ratio of 1:10, adjusting the pH value of the mixed solution of the chitosan and the acetic acid solution to 10, collecting precipitates to obtain pretreated chitosan, mixing the pretreated chitosan and isopropanol according to the mass ratio of 1:8, adding a glycidol trimethyl ammonium chloride solution with the mass fraction of 20% and the mass fraction of 4 times that of the pretreated chitosan, stirring for reaction, adding ethanol for precipitation, filtering to obtain filter residues, drying the filter residues at the temperature of 75 ℃ for 3 hours to obtain chitosan quaternary ammonium salt, mixing the chitosan quaternary ammonium salt and water according to the mass ratio of 1:20, adding citric acid with the mass of 0.2 time of that of the chitosan quaternary ammonium salt and triethanolamine with the mass of 0.3 time of the chitosan quaternary ammonium salt, and stirring and mixing to obtain the chitosan quaternary ammonium salt treatment solution.
Preferably, the calcium ion solution in the step (4) is a calcium chloride solution with the mass fraction of 5%.
Example 4
A medical biological gel hemostatic dressing mainly comprises the following raw material components in parts by weight: 10 parts of chitosan quaternary ammonium salt, 8 parts of bacterial cellulose, 3 parts of polyacrylamide, 8 parts of sodium alginate, 12 parts of polyvinyl alcohol and 4 parts of nano-silver.
A preparation method of a medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) mixing bacterial cellulose and a polyvinyl alcohol solution with the mass fraction of 10% according to the mass ratio of 1:20, adding polyacrylamide with the mass of 0.4 time that of the bacterial cellulose, stirring and mixing for 40min at the temperature of 35 ℃ and the rotating speed of 280r/min to obtain a mixed dispersion liquid, freezing the mixed dispersion liquid for 6h at the temperature of-30 ℃, then unfreezing for 6h at the room temperature, and performing freeze-thaw cycling for 3 times to obtain a gel blank;
(2) mixing the gel blank obtained in the step (1) with a chitosan quaternary ammonium salt treatment solution according to a mass ratio of 1:8, standing and reacting for 4 hours at the temperature of 40 ℃, filtering to obtain a pretreated gel blank, mixing the pretreated gel blank with a silver ammonia solution with the mass fraction of 10% in a conical flask according to a mass ratio of 1:10, adding sodium alginate with the mass of 0.2 times that of the pretreated gel blank into the conical flask, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 180r/min, filtering, and freeze-drying;
(3) mixing the substance obtained in the step (2) with a calcium ion solution according to a mass ratio of 1:10, standing and reacting for 3 hours at the temperature of 35 ℃, filtering, and freeze-drying;
(4) and (4) performing index analysis on the substance obtained in the step (3).
Preferably, the chitosan quaternary ammonium salt treatment solution in the step (2) is prepared by mixing chitosan and an acetic acid solution with the mass fraction of 2% according to the mass ratio of 1:10, adjusting the pH value of the mixed solution of the chitosan and the acetic acid solution to 10, collecting precipitates to obtain pretreated chitosan, mixing the pretreated chitosan and isopropanol according to the mass ratio of 1:8, adding a glycidol trimethyl ammonium chloride solution with the mass fraction of 20% and the mass fraction of 4 times that of the pretreated chitosan, stirring for reaction, adding ethanol for precipitation, filtering to obtain filter residues, drying the filter residues at the temperature of 75 ℃ for 3 hours to obtain chitosan quaternary ammonium salt, mixing the chitosan quaternary ammonium salt and water according to the mass ratio of 1:20, adding citric acid with the mass of 0.2 time of that of the chitosan quaternary ammonium salt and triethanolamine with the mass of 0.3 time of the chitosan quaternary ammonium salt, and stirring and mixing to obtain the chitosan quaternary ammonium salt treatment solution.
Preferably, the calcium ion solution in the step (3) is a calcium chloride solution with the mass fraction of 5%.
Comparative example
A medical biological gel hemostatic dressing mainly comprises the following raw material components in parts by weight: 8 parts of bacterial cellulose, 3 parts of polyacrylamide and 12 parts of polyvinyl alcohol.
A preparation method of a medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) mixing bacterial cellulose and a polyvinyl alcohol solution with the mass fraction of 10% according to the mass ratio of 1:20, adding polyacrylamide with the mass of 0.4 time that of the bacterial cellulose, stirring and mixing for 40min at the temperature of 35 ℃ and the rotating speed of 280r/min to obtain a mixed dispersion liquid, freezing the mixed dispersion liquid for 6h at the temperature of-30 ℃, then unfreezing for 6h at the room temperature, and performing freeze-thaw cycling for 3 times to obtain a gel blank;
(2) mixing the substance obtained in the step (1) with a calcium ion solution according to a mass ratio of 1:10, standing and reacting for 3 hours at the temperature of 35 ℃, filtering, and freeze-drying;
(3) and (3) carrying out index analysis on the substance obtained in the step (2).
Preferably, the calcium ion solution in the step (2) is a calcium chloride solution with the mass fraction of 5%.
Examples of effects
Table 1 below shows the analysis results of the bactericidal performance and the blood coagulation performance of the medical bio-gel hemostatic dressing using examples 1 to 4 of the present invention and the comparative example.
TABLE 1
From the comparison of the experimental data of example 1 and the comparative example in table 1, it can be found that the addition of chitosan quaternary ammonium salt, sodium alginate and silk during the preparation of the medical biogel hemostatic dressing can effectively improve the blood adsorbability of the product, promote the blood coagulation and enable the product to have excellent antibacterial performance; from the comparison of the experimental data of example 1 and example 2, it can be found that when sodium alginate is not added to the medical biogel hemostatic dressing, nano silver generated in the silver ammonia solution cannot be fixed in the product, so that the antibacterial performance of the product is reduced, and due to the lack of sodium alginate, the product cannot adsorb sufficient calcium ions when being mixed with the calcium ion solution, so that blood cannot be rapidly coagulated during the use process; compared with the experimental data of the embodiment 1 and the embodiment 3, the antibacterial property of the product is reduced due to the loss of the chitosan quaternary ammonium salt when the chitosan quaternary ammonium salt is not added in the product, and meanwhile, the sodium alginate cannot fix the nano silver in the product and the binding force between the sodium alginate and the gel blank is low when the chitosan quaternary ammonium salt is not added in the product, so that the coagulation performance of the product is further reduced; from the comparison of the experimental data of example 1 and example 4, it can be seen that when no silk is added to the product, the nano silver lacks a carrier, so that the antibacterial property of the product is reduced, and simultaneously, due to the absence of the silk, the calcium ion adsorption groups in the product are reduced, so that the blood coagulation performance of the product is further reduced.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (1)
1. A preparation method of a medical biogel hemostatic dressing mainly comprises the following preparation steps:
(1) mixing a silkworm cocoon and a 10% sodium carbonate solution according to a mass ratio of 1:8, stirring and reacting for 80min at a temperature of 60 ℃ and a rotating speed of 300r/min, filtering to obtain a degummed silkworm cocoon, crushing the degummed silkworm cocoon in a crusher for 40min to obtain refined silk, mixing the refined silk and a 3% trypsin solution according to a mass ratio of 1:12 in a beaker, stirring and reacting for 50min at a temperature of 36 ℃ and a rotating speed of 300r/min, heating the material in the beaker to 90 ℃, keeping the temperature for 30min, inactivating enzyme to obtain a fibroin dispersion liquid, and mixing the fibroin dispersion liquid and a 10% polyvinyl alcohol solution according to a mass ratio of 3: 1;
(2) mixing bacterial cellulose and the substance obtained in the step (1) according to a mass ratio of 1:20, adding polyacrylamide with the mass of 0.4 time that of the bacterial cellulose, stirring and mixing for 40min at a temperature of 35 ℃ and a rotating speed of 280r/min to obtain a mixed dispersion liquid, freezing the mixed dispersion liquid for 6h at a temperature of-30 ℃, then unfreezing for 6h at room temperature, and performing freeze-thaw cycling for 3 times to obtain a gel blank;
(3) mixing the gel blank obtained in the step (2) with a chitosan quaternary ammonium salt treatment solution according to a mass ratio of 1:8, standing and reacting for 4 hours at the temperature of 40 ℃, filtering to obtain a pretreated gel blank, mixing the pretreated gel blank and a silver ammonia solution with the mass fraction of 10% in a conical flask according to a mass ratio of 1:10, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 180r/min, filtering, and freeze-drying;
(4) mixing the substance obtained in the step (3) with a calcium ion solution according to a mass ratio of 1:10, standing and reacting for 3 hours at the temperature of 35 ℃, filtering, and freeze-drying;
(5) performing index analysis on the substance obtained in the step (4);
mixing chitosan and an acetic acid solution with the mass fraction of 2% according to the mass ratio of 1:10, adjusting the pH value of a mixed solution of the chitosan and the acetic acid solution to 10, collecting precipitates to obtain pretreated chitosan, mixing the pretreated chitosan and isopropanol according to the mass ratio of 1:8, adding a glycidol trimethyl ammonium chloride solution with the mass fraction of 20% and the mass fraction of 4 times that of the pretreated chitosan, stirring for reaction, adding ethanol for precipitation, filtering to obtain filter residues, drying the filter residues at the temperature of 75 ℃ for 3 hours to obtain chitosan quaternary ammonium salt, mixing the chitosan quaternary ammonium salt and water according to the mass ratio of 1:20, adding citric acid with the mass of 0.2 time of that of the chitosan quaternary ammonium salt and triethanolamine with the mass of 0.3 time of that of the chitosan quaternary ammonium salt, and stirring for mixing to obtain the chitosan quaternary ammonium salt treatment solution;
and (4) the calcium ion solution in the step (4) is a calcium chloride solution with the mass fraction of 5%.
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| CN110522945B (en) | 2020-07-03 |
| CN111632190B (en) | 2021-10-19 |
| CN111632190A (en) | 2020-09-08 |
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