CN111760903A - Method for repairing soil polycyclic aromatic hydrocarbon pollution by bacterium and plant combination - Google Patents
Method for repairing soil polycyclic aromatic hydrocarbon pollution by bacterium and plant combination Download PDFInfo
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- CN111760903A CN111760903A CN202010597241.4A CN202010597241A CN111760903A CN 111760903 A CN111760903 A CN 111760903A CN 202010597241 A CN202010597241 A CN 202010597241A CN 111760903 A CN111760903 A CN 111760903A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Abstract
The invention provides a method for remedying soil polluted by polycyclic aromatic hydrocarbon by combining bacteria and plants, belonging to the technical field of soil remediation, and the method comprises the following steps: 1) applying the comamonas testosteroni microbial inoculum to soil to be repaired for 25-35 days; 2) and then planting seeds of the gramineous forage grass in soil, mowing after the gramineous forage grass is mature, measuring the content of the polycyclic aromatic hydrocarbon in the soil, and applying the action of the testosterone comamonas bacterium agent for 25-35 days when the content of the polycyclic aromatic hydrocarbon in the soil is more than or equal to 1mg/kg until the content of the polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6mg/kg, thereby completing restoration. The method has high degradation rate of the polycyclic aromatic hydrocarbon, and can recover the ecological system of the soil in the seriously polluted area while degrading the polycyclic aromatic hydrocarbon for a long time.
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to a method for remediating soil polluted by polycyclic aromatic hydrocarbon through combined bacterial and plant remediation.
Background
Polycyclic aromatic hydrocarbons are volatile, hydrophobic hydrocarbons consisting of two or more fused together benzene rings. The polycyclic aromatic hydrocarbon fuel is widely existed in the environment and is difficult to degrade, and a large amount of polycyclic aromatic hydrocarbon is generated by incomplete combustion, high-temperature pyrolysis, boiler use, automobile exhaust emission and the like of chemical fuels in oil exploitation and processing, industrial activities, wherein the combustion of the fossil fuel is a main source of the polycyclic aromatic hydrocarbon in the environment. Polycyclic aromatic hydrocarbons in the atmosphere are transferred to soil in the modes of natural sedimentation, rainfall, snowfall and the like, and more than 90% of polycyclic aromatic hydrocarbons in the environment are accumulated in the soil due to the adsorption effect of organic substances in the soil and the retention in the soil. Therefore, soil is the main medium for deposition, degradation and volatilization of polycyclic aromatic hydrocarbons in ecosystems. Due to the fact that polycyclic aromatic hydrocarbon has carcinogenic, teratogenic and mutagenic properties, the polycyclic aromatic hydrocarbon in various distributions is absorbed by plants, enters a food chain, and causes harm to human health through biological enrichment and the expansion of the food chain.
The traditional soil polycyclic aromatic hydrocarbon remediation method comprises a high thermal desorption method, an advanced oxidation method and a bioremediation method, and because the thermal desorption method has high cost, air pollution is easy to form; the advanced oxidation method is easy to cause secondary pollution to soil due to the addition of a large amount of chemical agents, so that the application of the method is greatly limited. Phytoremediation and microbiological methods circumvent the above drawbacks and are of great interest. However, due to the phytoremediation method, the growth cycle is too long, and the polycyclic aromatic hydrocarbon is difficult to dissolve in water and is not easy to be absorbed and metabolized by plants, and the remediation effect is not ideal. The microbial remediation method can effectively open the ring of the polycyclic aromatic hydrocarbon and improve the solubility of pollutants, but the method has short duration and cannot restore the soil polluted by the polycyclic aromatic hydrocarbon with high concentration for a long time.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for remedying soil polluted by polycyclic aromatic hydrocarbons by combining bacteria and plants, wherein the method has a high degradation rate of polycyclic aromatic hydrocarbons, and can restore the ecosystem of the soil in severely polluted areas while degrading polycyclic aromatic hydrocarbons for a long time.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a method for remedying soil polluted by polycyclic aromatic hydrocarbon by combining bacteria and plants, which comprises the following steps:
1) applying the comamonas testosteroni microbial inoculum to soil to be repaired for 25-35 days;
2) then planting seeds of the gramineous forage grass in the soil, and mowing after the gramineous forage grass is mature;
measuring the content of polycyclic aromatic hydrocarbon in the soil, and completing restoration when the content of polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6 mg/kg;
and when the content of the polycyclic aromatic hydrocarbon in the soil is more than or equal to 1mg/kg, applying the action of the testosterone comamonas microbial inoculum for 25-35 days again to measure the content of the polycyclic aromatic hydrocarbon in the soil, and when the content of the polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6mg/kg, completing the restoration.
Preferably, the application amount of the comamonas testosteroni microbial inoculum in the step 1) is (8.5-9.5) × 1012cfu per square meter.
Preferably, the depth of the application of the comamonas testosteroni microbial inoculum into the soil is 5-10 cm.
Preferably, the Comamonas testosteroni microbial inoculum in the step 1) is solid bacterial powder or liquid microbial inoculum, and the viable count of the solid bacterial powder is more than or equal to 7.5 × 1011cfu/g, the viable count of the liquid microbial inoculum is more than or equal to 5 × 109cfu/ml。
Preferably, the grass of the grass family in the step 2) includes one or more of leymus chinensis, setaria viridis and lysimachia.
Preferably, the seeding density of the gramineous pasture is 500-1000 plants per square meter.
Preferably, the mowing frequency is 2-4 times.
Preferably, in the step 1), the content of polycyclic aromatic hydrocarbon in the soil to be repaired is 5-20 mg/kg, and the content of organic matters in the soil to be repaired is 20-100 g/kg.
The invention has the beneficial effects that: the invention provides a method for remedying soil polluted by polycyclic aromatic hydrocarbon by combined bacteria and plants, which comprises the steps of firstly, primarily degrading the polycyclic aromatic hydrocarbon with higher concentration in the soil by using a testosterone comamonas microbial inoculum, and then remedying the soil by using gramineous forage as a phytoremediation material; the two are mutually matched and act together, so that the degradation effect of the polycyclic aromatic hydrocarbon is improved, and the degradation time of the polycyclic aromatic hydrocarbon is shortened. The invention takes gramineous forage grass as a phytoremediation material, and has the characteristics of fast growth cycle, high actual economic value, higher tolerance to polycyclic aromatic hydrocarbon and the like. After the gramineous forage grass is mowed, polycyclic aromatic hydrocarbon content in soil is used as a microbial inoculum to add a reference node, so that the degradation rate of the polycyclic aromatic hydrocarbon is improved, and the long-acting and durable degradation of the polycyclic aromatic hydrocarbon is achieved by combining the growth of the gramineous forage grass.
The method provided by the invention has strong pertinence, the degradation rate of the soil polycyclic aromatic hydrocarbon is obviously improved, and the grassland ecosystem of the soil in the seriously polluted area can be recovered while the polycyclic aromatic hydrocarbon is degraded for a long time.
Detailed Description
The invention provides a method for remedying soil polluted by polycyclic aromatic hydrocarbon by combining bacteria and plants, which comprises the following steps: 1) applying the comamonas testosteroni microbial inoculum to soil to be repaired for 25-35 days; 2) planting seeds of gramineous forage grass in the soil, mowing after the gramineous forage grass is mature, measuring the content of polycyclic aromatic hydrocarbon in the soil, and finishing restoration when the content of the polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6 mg/kg; and when the content of the polycyclic aromatic hydrocarbon in the soil is more than or equal to 1mg/kg, applying the action of the testosterone comamonas microbial inoculum for 25-35 days again to measure the content of the polycyclic aromatic hydrocarbon in the soil, and when the content of the polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6mg/kg, completing the restoration.
In the invention, firstly, the comamonas testosteroni microbial inoculum is applied to soil to be repaired for 25-35 days, and the application amount of the comamonas testosteroni microbial inoculum is preferably (8.5-9.5) × 1012cfu/square meter, more preferably (8.8-9.2) × 1012cfu/square meter, most preferably 9.0 × 1012cfu/square meter; in the invention, the depth of the application of the comamonas testosteroni microbial inoculum into soil is preferably 5-10 cm, and more preferably 6-8 cm; in the invention, the comamonas testosteroni microbial inoculum is solid microbial inoculum or liquid microbial inoculum; the viable count of the solid bacterial powder is preferably more than or equal to 7.5×1011cfu/g, the viable count of the liquid microbial inoculum is preferably more than or equal to 5 × 109cfu/ml. The source and preparation method of the comamonas testosteroni are not particularly limited, and the comamonas testosteroni can be prepared by adopting conventional commercial products or self-culture in the field. In the practice of the present invention, the Comamonas testosteroni is preferably Comamonas testosteroni (ACCC 02640); the preferred testosterone comamonas is preserved in the agricultural microorganism center of China Committee for culture Collection of microorganisms (China agricultural microorganism culture preservation management center, address 12 south China Union of Guancun in Hakken district, Beijing), in the invention, the acting time is preferably 28-32 d, more preferably 30d, after the testosterone comamonas acts on the microbial inoculum, the polycyclic aromatic hydrocarbon content in the soil is preferably determined, in the invention, the polycyclic aromatic hydrocarbon content determination is preferably performed by adopting a liquid chromatography-mass spectrometry or a gas chromatography-mass spectrometry, the specific operation of the polycyclic aromatic hydrocarbon content determination is not specially limited, the conventional operation in the field is adopted, in the invention, when the preferred polycyclic aromatic hydrocarbon content in the soil is 3-10 mg/kg, sowing seeds of gramineous forage grass; and when the content of the polycyclic aromatic hydrocarbon in the soil is more than 10mg/kg, applying the testosterone comamonas microbial inoculum again for acting.
After the action of the comamonas testosteroni microbial inoculum, seeds of gramineous forage grass are planted in the soil. In the invention, the grass of the Gramineae preferably comprises one or more of leymus chinensis, green bristlegrass and lysimachia; the seeding density of the gramineous forage grass is preferably 500-1000 plants per square meter, more preferably 600-900 plants per square meter, and in the invention, the seeding quantity of seeds of the gramineous forage grass is preferably 5-10 g per square meter, more preferably 6-9 g per square meter; in the present invention, the planting is preferably broadcast sowing. The growth management of the gramineous forage grass is not particularly limited, and the forage grass can be grown by adopting the conventional forage grass in the field.
The forage grass is mowed after the forage grass is mature, the content of polycyclic aromatic hydrocarbon in soil is measured, and when the content of polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6mg/kg, the restoration is completed; and when the content of the polycyclic aromatic hydrocarbon in the soil is more than or equal to 1mg/kg, applying the action of the testosterone comamonas microbial inoculum for 25-35 days again to measure the content of the polycyclic aromatic hydrocarbon in the soil, and when the content of the polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6mg/kg, completing the restoration. In the present invention, the specific operation of applying the comamonas testosteroni microbial inoculum again is the same as the operation of applying the comamonas testosteroni microbial inoculum for the first time, and is not described herein again; in the present invention, the application amount of the comamonas testosteroni agent to be applied again is preferably smaller than that of the comamonas testosteroni agent to be applied for the first time. In the present invention, if the soil remediation criteria described above (i.e., polycyclic aromatic hydrocarbon content in soil < 1mg/kg, and benzo [ a ] pyrene toxicity equivalent concentration in soil < 0.6mg/kg) are not met), the application of the comamonas testosteroni agent is preferably repeated a plurality of times. The mowing frequency of the gramineous forage grass is not specially limited, the mowing frequency is determined according to a soil restoration result, and if the mowing frequency does not reach the restoration standard, the gramineous forage grass is repeatedly mowed after being mature.
In the invention, the polycyclic aromatic hydrocarbon content is the total content of 16 polycyclic aromatic hydrocarbons, including naphthalene, phenanthrene, fluorene, pyrene, chrysene, anthracene, acenaphthene, fluoranthene, acenaphthylene, benzo [ a ] pyrene, benzo [ k ] fluoranthene, benzo [ a ] anthracene, benzo [ b ] fluoranthene, indeno [1,2,3-cd ] pyrene, dibenzo [ a, h ] anthracene and benzo [ g, h, i ] perylene. The toxicity mechanism of the polycyclic aromatic hydrocarbon is similar, wherein benzo [ a ] pyrene has strong carcinogenicity and definite toxicological characteristics in soil, the toxicity of the mixed polycyclic aromatic hydrocarbon can be determined according to the toxicity equivalent concentration of the benzo [ a ] pyrene, and the potential carcinogenic risk in the area can be evaluated. The safety limit value of the toxicity equivalent concentration of the benzo [ a ] pyrene is 0.6mg/kg, and the toxicity equivalent concentration of the benzo [ a ] pyrene in the soil is less than 0.6mg/kg, which indicates that the area has no potential carcinogenic risk. The soil benzo [ a ] pyrene toxicity equivalent concentration calculation formula is as follows:
BaPeq＝∑(Ci×TEFi)
in the formula: ci is the measured concentration of substance i, and TEFi is the toxicity equivalent factor of substance i.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Selecting seeds of forage grass (Leymus chinensis L. of Gramineae), and Comamonas testosteroni solid powder with viable count of 7.8 × 1011cfu/g, and the soil remediation is carried out on the polycyclic aromatic hydrocarbon polluted soil around the Zhaodong Wuli grassland highway.
And (3) detecting the soil to be repaired: the total content of 16 polycyclic aromatic hydrocarbons is detected according to 3550B standard (USEPA, 1996), and the soil sample to be detected is frozen, dried and ground and screened by a 100-mesh standard sample. Polycyclic aromatic hydrocarbon extraction is carried out by using an ASE solid phase extractor, and an extraction solvent of dichloromethane/acetone (1:1, v/v) is added into a top collection bottle for 1L at first, and the mixture is rinsed for 2-3 times. The extraction conditions are that the temperature is 100 ℃, the pressure is 10.34kPa, the static extraction is carried out for 5min, the circulation is carried out for 2 times, the solvent leaches 60 percent of the volume of the pool, and the nitrogen purging is carried out for 1 min. Cleaning the extraction tank, screwing down a bottom cover, adding a filter membrane, placing a special funnel at the top, adding 10g of soil sample and 10g of diatomite respectively, uniformly mixing, screwing down a top cover, placing an ASE instrument, and obtaining an extraction liquid after the operation is finished. About 100mL of the obtained extract was rotary evaporated at 30 ℃ to obtain a concentrated solution when the sample was concentrated to 2 mL. Purifying the concentrated solution by using a magnesium silicate column, leaching the magnesium silicate column by using 4mL of dichloromethane, adding 5mL of n-hexane for soaking for 5min, then opening a control valve, adding 5mL of n-hexane, and discarding the effluent at the moment. Transferring the concentrated extract into a small column, adding 5mL of dichloromethane/n-hexane (1:9, v/v) solution, soaking for 2min, collecting liquid, concentrating again, transferring the sample to a gas-phase sample injection bottle after the sample is concentrated to be less than 0.5mL, adding 0.5mL of dichloromethane/n-hexane (1:9, v/v) solution, transferring the sample into the sample injection bottle, and fixing the volume to be 1 mL. 16 polycyclic aromatic hydrocarbons were detected by gas chromatography tandem mass spectrometry (GC-MS). The total content of 16 polycyclic aromatic hydrocarbons in the soil is 3.6mg/kg, and the content of each polycyclic aromatic hydrocarbon is shown in table 1. A soil organic matter content measurement adopts a burning method, air-dried soil is taken and burned for 4 hours at 550 ℃ of a muffle furnace, and the soil organic matter content is 26 g/kg.
TABLE 1 test of polycyclic aromatic hydrocarbons content in soil background
Adding testosterone comamonas microbial inoculum at the beginning of 5 months, wherein the dose of inoculated solid powder per square meter is 12 g; taking a soil sample after 30 days of treatment, and determining the content of the residual polycyclic aromatic hydrocarbon in the soil to be 2.8mg/kg by using a gas chromatography-tandem mass spectrometer;
sowing Chinese wildrye seeds in the last ten days of 6 months, and sowing 5g seeds in each square meter of soil; the Chinese wildrye grows seedlings after 14 days, and the number of the seedlings is about 700 per square meter after the seedlings grow;
after the Chinese wildrye grows to 120 days and is mature, and the biomass is high, the Chinese wildrye is mown;
and sampling again to determine the polycyclic aromatic hydrocarbon content of the soil, wherein the total polycyclic aromatic hydrocarbon content is 0.8mg/kg and is lower than 1mg/kg, and the toxicity equivalent concentration of benzo [ a ] pyrene is 0.15mg/kg and is lower than 0.6 mg/kg. Namely, the bacteria-plant combined soil remediation of the leymus chinensis and testosterone comamonas coupled polycyclic aromatic hydrocarbon polluted soil is completed.
Example 2
Selecting seeds of forage grass (Leymus chinensis L. of Gramineae), and Comamonas testosteroni solid powder with viable count of 7.8 × 1011cfu/g, and the soil remediation is carried out on the polycyclic aromatic hydrocarbon contaminated soil at a position of about 300m of Daqing oil drilling well.
And (3) soil to be restored: the total content of 16 polycyclic aromatic hydrocarbons is 9.5mg/kg, and the organic matter content is 21 g/kg. Adding testosterone comamonas microbial inoculum at the beginning of 5 months, wherein the addition amount of solid powder is 12g per square meter; taking a soil sample after 30 days of treatment, and determining the content of the residual polycyclic aromatic hydrocarbon in the soil to be 4.8mg/kg by using a gas chromatography-tandem mass spectrometer;
5, sowing Chinese wildrye seeds in the last ten days of the month, and sowing 5g seeds in each square meter of soil; the Chinese wildrye grows seedlings after 14 days, and the number of the seedlings is about 700 per square meter after the seedlings grow;
the Chinese wildrye is ripe after growing for 120 days, the biomass is high, and the Chinese wildrye is mowed in late 8 months;
sampling a mown leymus chinensis mown soil sample, measuring the content of polycyclic aromatic hydrocarbons in the soil, wherein the content of the total polycyclic aromatic hydrocarbons is 3.5mg/kg, applying the comamonas testosteroni microbial inoculum again, and the addition amount of solid powder per square meter is 10 g.
And (3) taking a soil sample after 30 days of inoculation to measure the content of the polycyclic aromatic hydrocarbon, wherein the total content of the polycyclic aromatic hydrocarbon is 0.73mg/kg and is lower than 1mg/kg, and the toxicity equivalent concentration of the benzo [ a ] pyrene is 0.18mg/kg and is lower than 0.6 mg/kg. Namely, the bacteria-plant combined soil remediation of the leymus chinensis and testosterone comamonas coupled polycyclic aromatic hydrocarbon polluted soil is completed.
Comparative example
The method for repairing polycyclic aromatic hydrocarbon contaminated soil by singly using three modes of coupling gramineous forage grass, testosterone comamonas, gramineous forage grass and testosterone comamonas comprises the following steps:
selecting seeds of forage grass (Leymus chinensis L. of Gramineae), and Comamonas testosteroni solid powder with viable count of 7.8 × 1011cfu/g, and repairing the polycyclic aromatic hydrocarbon soil at about 1km of Daqing oil drilling wells.
And (3) detecting the soil to be repaired: the total content of 16 polycyclic aromatic hydrocarbons in the soil is 6.9mg/kg, and the content of organic matters is 24 g/kg.
Setting soil sample plots to be restored as three treatment groups, namely a group A: and (3) microbial inoculum remediation group and B group: leymus chinensis repair group, C: and (5) a bacterium-plant coupling repairing group.
At the beginning of 5 months, A, C two groups of sample plot soil are put into testosterone comamonas bacterium agent, the addition amount of solid powder is 12g per square meter, and the depth of the bacterium agent addition soil layer is 10 cm; sowing leymus chinensis seeds in the soil of the sample plot of the group B, and sowing 5g seeds in each square meter of soil; collecting a soil sample after 30 days, and determining the content of the residual polycyclic aromatic hydrocarbon in the soil by using a gas chromatography tandem mass spectrometer, wherein the residual amounts of A, B, C three groups of soil PAHs are respectively 3.8mg/kg, 5.6mg/kg and 3.7 mg/kg;
sowing leymus chinensis seeds at the beginning of 6 months on a group C sample plot treated by the microbial inoculum for 30 days, sowing 5g of leymus chinensis seeds in each square meter of soil, forming seedlings after 14 days, and performing coupling repair on leymus chinensis and comamonas testosteroni, wherein 700 strains are grown in each square meter after the seedlings are formed;
after the Chinese wildrye grows for more than 120 days in the B, C groups, the Chinese wildrye is mature and has high biomass, and the Chinese wildrye in the two treatment groups is mowed at the beginning of 9 months;
and (3) measuring the content of the polycyclic aromatic hydrocarbon in the three treated soil samples, wherein the content of the polycyclic aromatic hydrocarbon in A, B, C treated soil is respectively as follows: 1.48mg/kg of microbial inoculum restoration, 1.8mg/kg of plant restoration and 0.71mg/kg of bacterial plant restoration; the toxicity equivalent concentration of benzo [ a ] pyrene in soil of each treatment group is respectively as follows: the microbial inoculum repairs 0.14mg/kg, the plants repairs 0.26mg/kg, the bacterial plants repairs 0.13mg/kg, and the experimental results show that the coupling repair of the leymus chinensis and the comamonas testosteroni has the synergistic effect, and the repair effect is obviously better than that of the single repair of the leymus chinensis and the comamonas testosteroni.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A method for remedying soil polluted by polycyclic aromatic hydrocarbon by bacterium and plant combination comprises the following steps:
1) applying the comamonas testosteroni microbial inoculum to soil to be repaired for 25-35 days;
2) then planting seeds of the gramineous forage grass in the soil, and mowing after the gramineous forage grass is mature;
measuring the content of polycyclic aromatic hydrocarbon in the soil, and completing restoration when the content of polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6 mg/kg;
and when the content of the polycyclic aromatic hydrocarbon in the soil is more than or equal to 1mg/kg, applying the action of the testosterone comamonas microbial inoculum for 25-35 days again to measure the content of the polycyclic aromatic hydrocarbon in the soil, and when the content of the polycyclic aromatic hydrocarbon in the soil is less than 1mg/kg and the toxicity equivalent concentration of benzo [ a ] pyrene in the soil is less than 0.6mg/kg, completing the restoration.
2. The method as claimed in claim 1, wherein the comamonas testosteroni agent is applied in step 1) in an amount of (8.5-9.5) × 1012cfu per square meter.
3. The method according to claim 2, wherein the depth of application of the comamonas testosteroni preparation into the soil is 5-10 cm.
4. The method according to claim 2 or 3, wherein the Comamonas testosteroni microbial inoculum in step 1) is solid microbial inoculum or liquid microbial inoculum, and the viable count of the solid microbial inoculum is more than or equal to 7.5 × 1011cfu/g, the viable count of the liquid microbial inoculum is more than or equal to 5 × 109cfu/ml。
5. The method according to claim 1, wherein the grass of step 2) comprises one or more of guinea grass, green bristlegrass and lysimachia.
6. The method according to claim 5, wherein the gramineous forage grass has a seeding density of 500 to 1000 plants per square meter.
7. The method according to claim 1, wherein the number of mows is 2-4.
8. The method according to claim 1, wherein in the step 1), the content of polycyclic aromatic hydrocarbon in the soil to be repaired is 5-20 mg/kg, and the content of organic matters in the soil to be repaired is 20-100 g/kg.
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