CN111748428A - Selenium-rich cannabidiol health beer and preparation method thereof - Google Patents
Selenium-rich cannabidiol health beer and preparation method thereof Download PDFInfo
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- CN111748428A CN111748428A CN202010669387.5A CN202010669387A CN111748428A CN 111748428 A CN111748428 A CN 111748428A CN 202010669387 A CN202010669387 A CN 202010669387A CN 111748428 A CN111748428 A CN 111748428A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
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- C12C7/053—Preparation or treatment of the mash part of the mash being non-cereal material
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
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Abstract
The invention relates to a selenium-rich cannabidiol beer and a preparation method thereof, and the health care beer comprises the following components in percentage by weight: the health-care beer is rich in protein, vitamins, minerals and trace elements, can be used for maximally dissolving cannabidiol in the beer, is rich in plant active selenium, achieves the national green organic selenium-rich food authentication standard and has a health-care function, and meanwhile, the active ingredients are absorbed by a human body in a liquid form, so that the absorption rate of the active ingredients is greatly improved.
Description
Technical Field
The invention relates to the technical field of beer processing, in particular to selenium-rich cannabidiol health-care beer and a preparation method thereof.
Background
The beer is brewed by using wheat malt and barley malt as main raw materials, adding hops, gelatinizing and saccharifying in a liquid state, and fermenting in a liquid state, wherein the alcohol content is between 3 and 4 percent, so that the beer is convenient to store and can improve the taste, a large amount of carbon dioxide is always contained in the beer, in addition, the beer is also rich in various amino acids, vitamins, moisture and various bioactive enzymes, the nutrients can promote digestion in a human body, and meanwhile, components missing in the human body are supplemented; the beer has strong absorption capacity to the effective components in the wine, so the beer is the most popular wine which is suitable for all ages and has wide sale routes.
However, the beer is extremely common in the life of people, so that the alertness of people is reduced, after a large amount of beer is drunk, the people often feel good fortune and big belly, although the content of alcohol in the beer is low, the drinking of the beer can also cause drunkenness, nausea and vomiting, and when the beer is drunk in a large amount, a plurality of diseases such as cardiovascular and cerebrovascular diseases, hypertension, hyperlipidemia and the like can be brought to organisms, even coma and the like, and the life is threatened; if the selenium-rich cannabidiol health-care beer can exist, the selenium-rich cannabidiol health-care beer not only has the same pure and refreshing taste as the conventional beer, but also has the health-care and health-care effects, and the selenium-rich cannabidiol health-care beer is a great contribution to the field of beer.
Since 1973, the trace element selenium has been identified as one of the essential trace elements by the expert committee of the world health organization, the selenium has been attracted by scholars and experts at home and abroad for nearly thirty years, and it has been proved that the diseases related to selenium deficiency of human beings reach over forty kinds, and the more prominent diseases comprise cardiovascular and cerebrovascular diseases, cancer, hepatitis, diabetes, senile cataract and the like. China has been on the international leading level in the research of selenium and keshan disease (local cardiomyopathy), selenium and cancer, selenium and aging resistance and the like. Selenium is present in various tissues and fluids of the human body, is a component of glutathione peroxidase, and has the main functions of eliminating peroxides and free radicals (in vivo waste) in vivo and protecting cell membranes from performing normal physiological functions. Therefore, the selenium deficiency of human body can cause dysfunction of some important organs, and various diseases occur.
Selenium enters human bodies through food chains, namely the selenium in the human bodies is related to local food herbs, the selenium content in local parent rocks and soil is low, the selenium content in the food is also low, and thus the selenium deficiency is caused by insufficient selenium intake of the human bodies. The selenium is deficient in twenty-two provinces and partial regions of the city in China, nearly hundred million people are in a selenium deficiency state, such as a keshan disease region in the keshan county in northeast China, a liver cancer high-incidence region in the Qiongdong of Jiangsu, a colorectal cancer high-incidence region in the Gansu county in Zhejiang province and the like, which are all related to selenium deficiency in the environment and food herbal medicine, and the regions are artificially supplemented with selenium (medicines), so that the prevention and treatment effects are achieved. At present, in the United states and other related countries, when solving the problem of insufficient selenium, oral sodium selenite tablets or selenium yeast and other medicines are adopted as a way of supplementing selenium. People assume that beer which is favored to drink and has rich nutrition is formed into selenium-rich beer for a long time, but an ideal production technology cannot be found.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides the selenium-rich cannabidiol health care beer and the preparation method thereof.
The technical problem solved by the invention can be realized by adopting the following technical scheme:
the selenium-rich cannabidiol health-care beer comprises the following components in percentage by weight: 8-38% of selenium-rich raw material, 0.1-0.3% of saccharifying enzyme, 0.1-0.4% of selenium-rich yeast, 0.2-1% of cannabidiol albumin, 0.1-0.3% of hop and the balance of mineral water.
Furthermore, the health-care beer also comprises 1.2-3.5 wt% of an auxiliary material, wherein the auxiliary material is edible sugar or xylitol.
Further, the selenium-rich raw materials comprise the following raw materials in percentage by weight: 40 to 55 percent of selenium-rich rye, 15 to 20 percent of selenium-rich wheat, 10 to 15 percent of selenium-rich corn, 5 to 10 percent of selenium-rich rice, 10 to 15 percent of konjac flour and 5 to 10 percent of whole grain.
Further, the selenium content in the selenium-rich rye is 0.20-0.30 mg/kg, the selenium content in the selenium-rich wheat is 0.18-0.28 mg/kg, the selenium content in the selenium-rich corn is 0.10-0.22 mg/kg, the selenium content in the selenium-rich rice is 0.05-0.20 mg/kg, the selenium content in the konjac flour is 0.25-0.35 mg/kg, and the selenium content in the whole grain is 0.05-0.20 mg/kg.
Preferably, the content of organic selenium in the selenium-enriched yeast accounts for more than 91% of the total selenium content.
Further, the mineral water is selenium-rich mineral water, and the selenium-rich mineral water contains 0.025-0.04 mg/kg of selenium.
Preferably, the pH of the mineral water is 7.1-8.6.
A method for preparing selenium-rich cannabidiol health beer comprises the following steps:
s1, crushing and sieving: pulverizing selenium-rich raw materials, and sieving;
s2, saccharification: adding mineral water and saccharifying enzyme into the crushed and sieved raw material S1 to saccharify to obtain saccharified juice;
s3, filtering: immediately filtering after the saccharification is finished, and collecting filtrate to obtain clear feed liquid;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in an ethanol solution, then dropwise adding the solution into the clear feed liquid obtained in the step S3 in a high-speed homogenization state, and then, homogenizing the clear feed liquid under the pressure of 25-30 Mpa for 2-3 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary precipitation on the mixed solution for 5-15min, then cooling, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
Further, the weight ratio of the selenium-rich raw material to the mineral water in the step S2 is 1 (4.0-6.0).
Further, in the saccharification in the step S2, the mixture is heated at 45-52 ℃ for 50-60min, then heated to 60-65 ℃ for 25-30 min, then heated to 70-75 ℃ for 20-40 min, and finally heated to 78 ℃ to finish the saccharification.
Further, in the step S6, sterile air is adopted for oxygenation during cooling, the cooling speed of the mixed liquid is controlled, the mixed liquid is cooled to 8-10 ℃, and the cooling time is controlled to be 50-60 min.
The invention has the beneficial effects that:
1. compared with the prior art, the functional health care beer for synchronously preparing the cannabidiol containing selenium selects high-selenium raw materials, selenium element is naturally generated in the fermentation process, and the traditional step of adding functional components is reduced in the actual processing step, so that the method for preparing the two components in one step well saves the processing cost and is easy to industrialize, and meanwhile, the selenium element generated in the method belongs to plant extracts, so that the health of a circulation system can be comprehensively promoted, and the product has better effects of resisting anxiety disorder, autoimmune diseases, nervous system diseases and intestinal diseases; meanwhile, the biological activity of the human body can be obviously improved, the medicine has special curative effect on cardiovascular and cerebrovascular diseases, and plays a perfect health care role in insomnia, neurasthenia and endocrine dyscrasia.
2. The cannabidiol albumin used in the invention is water-soluble CBD albumin prepared from Cannabidiol (CBD) and albumin by a high-pressure homogenization technology, and on the premise of not changing the molecular structure of the cannabidiol, the water solubility, biocompatibility and bioavailability of the CBD are effectively improved, so that the CBD is dissolved in beer to the maximum extent and is absorbed by a human body in a liquid form, the effect of the CBD is fully exerted, and the absorption rate of active ingredients is improved.
3. The invention has the advantages of advanced brewing process, small equipment investment, low cost, easy large-scale production and better popularization.
The foregoing is a summary of the present invention, and in order to provide a clear understanding of the technical means of the present invention and to be implemented in accordance with the present specification, preferred embodiments of the present invention are described in detail below.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
This invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, which are provided for a complete and complete disclosure of the invention and to fully convey the scope of the invention to those skilled in the art.
Unless otherwise defined, terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Further, it will be understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense.
The invention provides selenium-enriched cannabidiol health care beer, which comprises the following components in percentage by weight: 8-38% of selenium-rich raw material, 0.1-0.3% of saccharifying enzyme, 0.1-0.4% of selenium-rich yeast, 0.2-1% of cannabidiol albumin, 0.1-0.3% of hop and the balance of mineral water.
Furthermore, the health-care beer also comprises 1.2-3.5 wt% of an auxiliary material, wherein the auxiliary material is edible sugar or xylitol.
Further, the selenium-rich raw materials comprise the following raw materials in percentage by weight: 40 to 55 percent of selenium-rich rye, 15 to 20 percent of selenium-rich wheat, 10 to 15 percent of selenium-rich corn, 5 to 10 percent of selenium-rich rice, 10 to 15 percent of konjac flour and 5 to 10 percent of whole grain.
Specifically, the selenium content in the selenium-rich rye is 0.20-0.30 mg/kg, the selenium content in the selenium-rich wheat is 0.18-0.28 mg/kg, the selenium content in the selenium-rich corn is 0.10-0.22 mg/kg, the selenium content in the selenium-rich rice is 0.05-0.20 mg/kg, the selenium content in the konjac flour is 0.25-0.35 mg/kg, and the selenium content in the whole grain is 0.05-0.20 mg/kg.
Further, the mineral water is selenium-rich mineral water, and the selenium-rich mineral water contains 0.025-0.04 mg/kg of selenium.
Specifically, each liter of mineral water contains the following minerals and trace elements: 0.5 to 20mg of sodium, 4 to 60 mg of calcium, 0.5 to 28mg of magnesium, 0.02 to 1.5 mg of zinc, 0.2 to 4mg of potassium, 0.05 to 0.29 mg of iron, 0.2 to 1.5 mg of iodine, 0.01 to 0.05 mg of selenium, 25 to 60 mg of metasilicic acid, less than 250 mg of sulfate radical, and 100 mg of soluble total solid.
Specifically, the PH of the mineral water is 7.1-8.6.
The invention also provides a preparation method of the selenium-rich cannabidiol health beer, which comprises the following steps:
s1, crushing and sieving: crushing the selenium-rich raw materials, and screening the crushed selenium-rich raw materials through a screen with 60-80 meshes for later use; although the pulverization is a simple mechanical process, the pulverization degree is very important for biochemical change of saccharification, composition of raw materials, filtering speed of juice obtained from the raw materials and utilization rate of the raw materials, so the pulverization degree is slightly finer, which is beneficial to leaching and dissolving of materials, is beneficial to free of enzyme and improves saccharification effect, but cannot be too fine; before crushing, a proper amount of water is added to wet the surface of the selenium-rich raw material for 5-10 minutes in advance to meet the requirement of crushing the powder without breaking, and the selenium-rich raw material is required to be used as the powder, is not suitable for long-time storage and is not allowed to stay overnight;
s2, saccharification: pulverizing selenium-rich materials, sieving, adding into a saccharifying pot, adding mineral water, hydrolyzing starch in rye, wheat, rice, etc. into saccharides such as maltose, etc. at certain temperature with saccharifying enzyme, and decomposing protein into amino acids, etc. which are easy to ferment and utilize by yeast; the saccharification specifically comprises:
s201, feeding of a saccharifying pot: starting the saccharifying pot for stirring, opening a powder bin valve, putting the crushed malt flour into the saccharifying pot, uniformly stirring, and stopping stirring when the temperature reaches the set temperature.
S202 protein arrest: stopping stirring when the temperature reaches 52 ℃, and standing and preserving heat for 50-60min for protein decomposition.
S203, saccharification: after the protein decomposition is finished, starting a saccharification pot for stirring, opening steam for heating, heating to a set temperature (60-65 ℃) at a speed of 1-1.5 ℃/min for saccharification, wherein the saccharification time is about 25-30 min;
s204, finally raising the temperature to 78 ℃, and preparing for filtering;
s3, filtering: immediately filtering after the saccharification is finished, and collecting filtrate to obtain clear feed liquid;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in a small amount of 50% ethanol solution for demulsification, slowly dripping the demulsified cannabidiol albumin into the clarified feed liquid obtained in the step S3, homogenizing at the rotating speed of 5000-7000 r/min for 5-15min, and then, introducing the homogenized feed liquid into a homogenizer for homogenizing at the pressure of 25-30 MPa for 2-3 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary precipitation on the mixed solution for 5-15min, then cooling, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
Further, the weight ratio of the selenium-rich raw material to the mineral water in the step S2 is 1 (4.0-6.0).
Further, in the saccharification in the step S2, the mixture is heated at 45-52 ℃ for 50-60min, then heated to 60-65 ℃ for 25-30 min, then heated to 70-75 ℃ for 20-40 min, and finally heated to 78 ℃ to finish the saccharification.
Further, when the mixed liquid is cooled in the step S6, sterile air is used for oxygenation, the cooling speed of the mixed liquid is controlled, the mixed liquid is cooled to 8-10 ℃, and the cooling time is controlled to be 50-60 min.
Example 1:
the embodiment provides selenium-enriched cannabidiol health care beer which comprises the following components in percentage by weight: 19% of selenium-rich raw material, 0.3% of saccharifying enzyme, 0.4% of selenium-rich yeast, 0.2% of cannabidiol albumin, 0.3% of hop, 1.8% of xylitol and 78% of mineral water.
Further, the selenium-rich raw materials comprise the following raw materials in percentage by weight: 45% of selenium-rich rye, 20% of selenium-rich wheat, 12% of selenium-rich corn, 8% of selenium-rich rice, 12% of konjac flour and 8% of whole grain.
The preparation method of the health-care beer comprises the following steps:
s1, crushing and sieving: crushing the selenium-rich raw material and screening the selenium-rich raw material by a 70-mesh screen for later use;
s2, saccharification: adding mineral water and diastase into the crushed and sieved raw material S1 for saccharification, wherein the saccharification is to heat the raw material at 45-52 ℃ for 60min, then heat the raw material to 60-65 ℃ for 30min, then heat the raw material to 70-75 ℃ for 30min, finally heat the raw material to 78 ℃ for saccharification to obtain saccharified juice;
s3, filtering: immediately filtering with a 70-mesh screen after saccharification to obtain clear juice;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in an ethanol solution, slowly dropwise adding the solution into the clarified liquid obtained in the step S3, homogenizing at a rotating speed of 6000r/min for 10min, and then inoculating the homogenized liquid into a homogenizer, and homogenizing under 30Mpa for 3 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary sedimentation on the mixed solution for 10min, then cooling, carrying out oxygenation by adopting sterile air during cooling, controlling the cooling speed of the mixed solution to ensure that the mixed solution is cooled to 8-10 ℃, taking the cooled mixed solution as the initial fermentation temperature, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
The beer obtained in the embodiment is clear and transparent, has dense bubbles, obvious hop aroma and no peculiar smell, and has no visible impurities.
Example 2:
the embodiment provides selenium-enriched cannabidiol health care beer which comprises the following components in percentage by weight: 16% of selenium-rich raw material, 0.2% of saccharifying enzyme, 0.2% of selenium-rich yeast, 0.5% of cannabidiol albumin, 0.2% of hop, 2.9% of xylitol and 80% of mineral water.
Further, the selenium-rich raw materials comprise the following raw materials in percentage by weight: 40% of selenium-rich rye, 18% of selenium-rich wheat, 15% of selenium-rich corn, 7% of selenium-rich rice, 15% of konjac flour and 5% of whole grain.
The preparation method of the health-care beer comprises the following steps:
s1, crushing and sieving: pulverizing selenium-rich raw materials, and sieving with 60 mesh sieve;
s2, saccharification: adding mineral water and diastase into the crushed and sieved raw material S1 for saccharification, wherein the saccharification is to heat the raw material at 45-52 ℃ for 60min, then heat the raw material to 60-65 ℃ for 30min, then heat the raw material to 70-75 ℃ for 30min, finally heat the raw material to 78 ℃ for saccharification to obtain saccharified juice;
s3, filtering: immediately filtering with a 70-mesh screen after saccharification to obtain clear juice;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in a small amount of 50% ethanol solution for demulsification, slowly dripping the demulsified cannabidiol albumin into the clarified feed liquid obtained in the step S3, homogenizing at the rotating speed of 5000r/min for 12min, and then inoculating the homogenized feed liquid into a homogenizer for homogenizing under the condition of 30MPa for 2 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary sedimentation on the mixed solution for 10min, then cooling, carrying out oxygenation by adopting sterile air during cooling, controlling the cooling speed of the mixed solution to ensure that the mixed solution is cooled to 8-10 ℃, taking the cooled mixed solution as the initial fermentation temperature, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
Example 3:
the embodiment provides selenium-enriched cannabidiol health care beer which comprises the following components in percentage by weight: 15% of selenium-rich raw material, 0.1% of saccharifying enzyme, 0.2% of selenium-rich yeast, 0.6% of cannabidiol albumin, 0.1% of hop, 1.5% of xylitol and 82.5% of mineral water.
Further, the selenium-rich raw materials comprise the following raw materials in percentage by weight: 50% of selenium-rich rye, 15% of selenium-rich wheat, 13% of selenium-rich corn, 7% of selenium-rich rice, 10% of konjac flour and 5% of whole grain.
The preparation method of the health-care beer comprises the following steps:
s1, crushing and sieving: pulverizing selenium-rich raw materials, and sieving with 80 mesh sieve;
s2, saccharification: adding mineral water and diastase into the crushed and sieved raw material S1 for saccharification, wherein the saccharification is to heat the raw material at 45-52 ℃ for 60min, then heat the raw material to 60-65 ℃ for 30min, then heat the raw material to 70-75 ℃ for 30min, finally heat the raw material to 78 ℃ for saccharification to obtain saccharified juice;
s3, filtering: immediately filtering with a 90-mesh screen after saccharification to obtain clear juice;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in a small amount of 50% ethanol solution for demulsification, slowly dripping the demulsified cannabidiol albumin into the clarified feed liquid obtained in the step S3, homogenizing at the rotating speed of 7000r/min for 5min, and then inoculating the homogenized feed liquid into a homogenizer for homogenizing under the condition of 30MPa for 3 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary sedimentation on the mixed solution for 10min, then cooling, carrying out oxygenation by adopting sterile air during cooling, controlling the cooling speed of the mixed solution to ensure that the mixed solution is cooled to 8-10 ℃, taking the cooled mixed solution as the initial fermentation temperature, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
The beer obtained in the embodiment is clear and transparent, has dense bubbles, obvious hop aroma and no peculiar smell, and has no visible impurities.
Example 4:
the embodiment provides selenium-enriched cannabidiol health care beer which comprises the following components in percentage by weight: 14% of selenium-rich raw material, 0.1% of saccharifying enzyme, 0.1% of selenium-rich yeast, 0.7% of cannabidiol albumin, 0.1% of hop, 20% of xylitol and 83% of mineral water.
Further, the selenium-rich raw materials comprise the following raw materials in percentage by weight: 49% of selenium-rich rye, 16% of selenium-rich wheat, 10% of selenium-rich corn, 6% of selenium-rich rice, 10% of konjac flour and 9% of whole grain cereal.
The preparation method of the health-care beer comprises the following steps:
s1, crushing and sieving: crushing the selenium-rich raw material and screening the selenium-rich raw material by a 70-mesh screen for later use;
s2, saccharification: adding mineral water and diastase into the crushed and sieved raw material S1 for saccharification, wherein the saccharification is to heat the raw material at 45-52 ℃ for 60min, then heat the raw material to 60-65 ℃ for 30min, then heat the raw material to 70-75 ℃ for 30min, finally heat the raw material to 78 ℃ for saccharification to obtain saccharified juice;
s3, filtering: after the saccharification is finished, placing the homogenized saccharified juice into a centrifugal barrel for centrifugation at 6000r/min for 10min, then removing precipitated impurities, and collecting clarified supernatant juice;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in a small amount of 50% ethanol solution for demulsification, slowly dripping the demulsified cannabidiol albumin into the clarified feed liquid obtained in the step S3, homogenizing at the rotating speed of 6000r/min for 10min, and then, inoculating the homogenized feed liquid into a homogenizer for homogenizing under the condition of 30MPa for 3 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary sedimentation on the mixed solution for 10min, then cooling, carrying out oxygenation by adopting sterile air during cooling, controlling the cooling speed of the mixed solution to ensure that the mixed solution is cooled to 8-10 ℃, taking the cooled mixed solution as the initial fermentation temperature, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
Example 5:
the embodiment provides selenium-enriched cannabidiol health care beer which comprises the following components in percentage by weight: 17% of selenium-rich raw material, 0.2% of saccharifying enzyme, 0.3% of selenium-rich yeast, 0.8% of cannabidiol albumin, 0.2% of hop, 2.5% of xylitol and 79% of mineral water.
Further, the selenium-rich raw materials comprise the following raw materials in percentage by weight: 45% of selenium-rich rye, 20% of selenium-rich wheat, 12% of selenium-rich corn, 8% of selenium-rich rice, 12% of konjac flour and 8% of whole grain.
S1, crushing and sieving: crushing the selenium-rich raw materials and screening the crushed selenium-rich raw materials through a 75-mesh screen for later use;
s2, saccharification: adding mineral water and diastase into the crushed and sieved raw material S1 for saccharification, wherein the saccharification is to heat the raw material at 45-52 ℃ for 60min, then heat the raw material to 60-65 ℃ for 30min, then heat the raw material to 70-75 ℃ for 30min, finally heat the raw material to 78 ℃ for saccharification to obtain saccharified juice;
s3, filtering: immediately filtering with a 70-mesh screen after saccharification to obtain clear juice;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in a small amount of 50% ethanol solution for demulsification, slowly dripping the demulsified cannabidiol albumin into the clarified feed liquid obtained in the step S3, homogenizing at the rotating speed of 6000r/min for 10min, and then, inoculating the homogenized feed liquid into a homogenizer for homogenizing under the condition of 30MPa for 3 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary sedimentation on the mixed solution for 10min, then cooling, carrying out oxygenation by adopting sterile air during cooling, controlling the cooling speed of the mixed solution to ensure that the mixed solution is cooled to 8-10 ℃, taking the cooled mixed solution as the initial fermentation temperature, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
The beer obtained in the embodiment is clear and transparent, has soft and dense bubbles, is hung in a cup for a long time, has pure hop aroma and no foreign flavor, is tasty and refreshing, is soft, has no peculiar smell, and has no suspended matters or precipitates.
Comparative examples
This embodiment is different from embodiment 5 in that:
(1) pulverizing selenium-rich raw materials, sieving with 60 mesh sieve, adding diastase, adding water, steaming, saccharifying, and continuously stirring; filtering the mixed saccharified juice after being homogenized by adopting a plate-and-frame filter membrane, and collecting clarified juice;
(2) slowly dripping water-soluble cannabidiol powder into the saccharified juice in a high-speed homogenate state, then inoculating into 30Mpa pressure for homogenizing for 3 cycles to form beer polymerized liquid, adding xylitol with formula amount, fermenting in a low-temperature environment, and canning after sterilization is finished.
The main physicochemical quality indexes of the beer comprise:
1. carbon dioxide: means the content of dissolved carbon dioxide in beer, which is produced during fermentation, which contributes to the foamability of beer and gives a pleasant, irritating feeling after drinking, the so-called kogation. Especially when drinking at about 15 ℃, carbon dioxide is gradually released, so that people can feel fresh and tasty, and the special bouquet flavor of beer can be smelled.
2. Bubble-holding property: generally, when beer is poured into a clean cup, foam rises, and the degree of foam persistence is referred to as foam retention. The beer with good quality has white and fine foam, long soaking time and good cup hanging performance, and gives people a pleasant feeling.
3. Turbidity: the apparent index of beer transparency is expressed in EBC turbidity unit, and the turbidity of good beer is very low and is below 0.5EBC unit. Poor beer can produce loss of light, even turbidity, which is a high value. The wine turbidity is caused by two reasons, one is biological turbidity caused by excessive bacteria, the wine cannot be drunk, the other is turbidity caused by cooling or oxidizing substances such as protein, polyphenol and the like of the wine in the storage process of the beer, the cold turbidity can disappear automatically after the beer is restored to the room temperature, and the turbidity does not influence the drinking, so that the consumers are recommended not to refrigerate the wine too low, generally at 10-15 ℃.
4. Alcohol content and original wort concentration: the alcohol content refers to the percentage content of alcohol in the wine, and can be expressed by volume percentage or mass percentage. The concentration of the raw wort is a value calculated according to the alcoholic strength and the real concentration in the beer by an empirical formula, and the value is used for expressing the amount of the raw wort.
The selenium-rich ferment beer obtained in each embodiment meets the following quality and technical requirements:
1. sensory index
External light: clear and transparent
Foaming: cup with dense bubbles and lasting hanging
And (3) taste: refreshing and fragrant
2. Physical and chemical indexes
According to the relevant standards in GB2758-2012 national food safety standard fermented wine and wine prepared by the fermented wine, the system detects the content of relevant parameters of the health beer in each embodiment, and the specific data are shown in Table 1.
TABLE 1 measurement results of health beer
| Alcohol content(degree) | Raw wort concentration/(° P) | turbidity/(EBC) | Bubble retention/s | Selenium content (mg/kg) | |
| Example 1 | 3.41 | 11.6 | 0.43 | 341 | 0.32 |
| Example 2 | 3.28 | 11.2 | 0.37 | 343 | 0.41 |
| Example 3 | 3.21 | 10.9 | 0.42 | 348 | 0.28 |
| Example 4 | 3.18 | 10.6 | 0.50 | 342 | 0.25 |
| Example 5 | 3.48 | 11.8 | 0.42 | 345 | 0.31 |
3. The total number of bacteria is less than or equal to 50cfu/ml, the coliform group is less than 3MPN/100ml, and pathogenic bacteria are not detected according to the microbial indexes (meeting the GB2758-2005 beer standard).
The mineralized water used in the invention contains 0.025-0.04 mg/kg of selenium, the main raw materials for brewing the health-care beer are selenium-rich rye, selenium-rich wheat, selenium-rich corn, selenium-rich rice and whole grains, and the health-care beer has the effects of nourishing yin, tonifying kidney, invigorating spleen, warming liver, improving eyesight and activating blood, is rich in vitamin B group components and 14 mineral substances necessary for human body, particularly contains iron and selenium, and also contains 17 amino acids, particularly 8 amino acids necessary for human body but incapable of being synthesized, particularly selenium is an indispensable microelement in human life, has the important functions of maintaining human body disease resistance, protecting heart and liver and preventing toxic substances from being generated in vivo, and has research and shows that the selenium is closely related to the selenium deficiency of more than 40 diseases such as human anemia, coronary heart disease, Kashin-Beck disease, diabetes, cancer and the like. The selenium-rich ferment beer disclosed by the invention is rich in plant active selenium besides rich nutrient components such as rye, wheat, corn, rice and grains, and can be scientifically taken by a human body, so that the product has better effects of resisting anxiety, autoimmune diseases, nervous system diseases and intestinal diseases; meanwhile, the biological activity of the human body can be obviously improved, the medicine has special curative effect on cardiovascular and cerebrovascular diseases, and has good health care effect on insomnia, neurasthenia and endocrine dyscrasia.
In addition, the raw material contains konjak powder, konjak is a natural high-fiber food, and the konjak glucomannan serving as the main component of the konjak powder is not digested in the stomach after being eaten, can effectively adsorb cholesterol and bile acid, and can inhibit the absorption of the cholesterol and the bile acid by intestinal tracts. Meanwhile, as the food can not be absorbed by saliva of people and digested and absorbed by amylopsin hydrolysis, the food has the functions of reducing fat, lowering blood pressure, cleaning stomach, relaxing bowels, preventing cancer and the like, and has the functions of promoting the secretion of intestinal enzymes, improving the activity of the intestinal enzymes and clearing deposited wastes on the intestinal wall. And the konjac mannan has the effects of resisting and neutralizing the effect of alcohol and protecting alcoholic liver injury, and is particularly embodied in the aspects of reducing the alcohol concentration in blood, regulating the generation of inflammatory mediators, protecting gastric mucosa and the like.
Therefore, the selenium-enriched ferment beer is rich in protein, vitamins, mineral substances and trace elements, has low alcohol content and low heat, does not generate the phenomenon that the common beer accumulates heat in vivo and fat accumulates to cause the obesity, the blood pressure and the blood fat to increase and easily cause cardiovascular and cerebrovascular diseases after being eaten, and has a plurality of health-care effects of supplementing selenium, removing food stagnation, strengthening the spleen and the stomach, moistening the lung, improving eyesight and the like, and the sub-health state is improved.
Claims (10)
1. The selenium-enriched cannabidiol health care beer is characterized by comprising the following components in percentage by weight: 8-38% of selenium-rich raw material, 0.1-0.3% of saccharifying enzyme, 0.1-0.4% of selenium-rich yeast, 0.2-1% of cannabidiol albumin, 0.1-0.3% of hop and the balance of mineral water.
2. The selenium-enriched cannabidiol health beer as claimed in claim 1, wherein: the health-care beer also comprises 1.2-3.5 wt% of an auxiliary material, wherein the auxiliary material is edible sugar or xylitol.
3. The selenium-enriched cannabidiol health beer as claimed in claim 1, wherein the selenium-enriched raw materials comprise the following raw materials by weight percentage: 40 to 55 percent of selenium-rich rye, 15 to 20 percent of selenium-rich wheat, 10 to 15 percent of selenium-rich corn, 5 to 10 percent of selenium-rich rice, 10 to 15 percent of konjac flour and 5 to 10 percent of whole grain.
4. The selenium-enriched cannabidiol health beer as claimed in claim 3, wherein: the selenium-rich rye contains 0.20-0.30 mg/kg of selenium, the selenium-rich wheat contains 0.18-0.28 mg/kg of selenium, the selenium-rich corn contains 0.10-0.22 mg/kg of selenium, the selenium-rich rice contains 0.05-0.20 mg/kg of selenium, the konjac flour contains 0.25-0.35 mg/kg of selenium, and the whole grain contains 0.05-0.20 mg/kg of selenium.
5. The selenium-enriched cannabidiol health beer as claimed in claim 1, wherein: the content of organic selenium in the selenium-enriched yeast accounts for more than 91% of the total selenium content.
6. The selenium-enriched cannabidiol health beer as claimed in claim 1, wherein: the mineral water is selenium-rich mineral water, and the selenium-rich mineral water contains 0.025-0.04 mg/kg of selenium.
7. The selenium-enriched cannabidiol health beer as claimed in claim 5, wherein: the pH value of the mineral water is 7.1-8.6.
8. A method for preparing a health beer of cannabidiol enriched in selenium comprising the health beer of cannabidiol enriched in selenium as claimed in any one of claims 1 to 7, wherein: the preparation method of the health-care beer comprises the following steps:
s1, crushing and sieving: pulverizing selenium-rich raw materials, and sieving;
s2, saccharification: adding mineral water and saccharifying enzyme into the crushed and sieved raw material S1 to saccharify to obtain saccharified juice;
s3, filtering: immediately filtering after the saccharification is finished, and collecting filtrate to obtain clear feed liquid;
s4, preparing polymerization saccharification liquid: dissolving cannabidiol albumin in an ethanol solution, then dropwise adding the solution into the clear feed liquid obtained in the step S3 in a high-speed homogenization state, and then, homogenizing the clear feed liquid under the pressure of 25-30 Mpa for 2-3 cycles to obtain a polymerization saccharification liquid;
s5, boiling: boiling the polymerization saccharification liquid, and adding auxiliary materials in the boiling process to obtain a mixed liquid;
s6, precipitation and fermentation: carrying out rotary precipitation on the mixed solution for 5-15min, then cooling, and adding selenium-enriched yeast for low-temperature fermentation;
s7, filtering and filling diatoms: centrifuging and multi-filtering the mature fermented product to remove yeast and macromolecular protein in the fermentation liquor, pasteurizing to obtain the health beer, and filling.
9. The method for preparing the selenium-enriched cannabidiol health beer as claimed in claim 8, wherein the method comprises the following steps: in the step S2, the weight ratio of the selenium-rich raw material to the mineral water is 1 (4.0-6.0).
10. The method for preparing the selenium-enriched cannabidiol health beer as claimed in claim 8, wherein the method comprises the following steps: in the step S2, saccharification is carried out by heating at 45-52 ℃ for 50-60min, heating to 60-65 ℃ for 25-30 min, heating to 70-75 ℃ for 20-40 min, and finally heating to 78 ℃ to finish saccharification;
and S6, when cooling, the mixed liquid is oxygenated by sterile air, the cooling speed of the mixed liquid is controlled, the mixed liquid is cooled to 8-10 ℃, and the cooling time is controlled to be 50-60 min.
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Application publication date: 20201009 |