CN111743935A - Traditional Chinese medicine composition for preventing and treating dengue fever virus and preparation method thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating dengue fever virus and preparation method thereof Download PDFInfo
- Publication number
- CN111743935A CN111743935A CN202010763694.XA CN202010763694A CN111743935A CN 111743935 A CN111743935 A CN 111743935A CN 202010763694 A CN202010763694 A CN 202010763694A CN 111743935 A CN111743935 A CN 111743935A
- Authority
- CN
- China
- Prior art keywords
- extract
- perilla
- preventing
- fever virus
- dengue fever
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/24—Mucus; Mucous glands; Bursa; Synovial fluid; Arthral fluid; Excreta; Spinal fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/535—Perilla (beefsteak plant)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Communicable Diseases (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Neurology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention is suitable for the technical field of medicines, and provides a traditional Chinese medicine composition for preventing and treating dengue fever virus and a preparation method thereof.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating dengue fever virus and a preparation method thereof.
Background
Dengue viruses are classified in the flaviviridae family, flaviviridae genus. Including 4 different serotypes of dengue virus, i.e., type I, II, III, IV. Dengue is an acute infectious disease caused by dengue virus and is transmitted mainly by aedes aegypti and aedes albopictus, which is infected by aedes biting the blood of a patient with viremia of virus. The clinical manifestations mainly include high fever, headache, muscle and joint pain, rash, swollen lymph nodes and leukopenia, and severe cases may show bleeding or shock, even death. In the early 2019, the WHO listed dengue diseases caused by dengue viruses as one of ten major threats facing the global health in 2019. From the beginning of 2019 to the end of 8 months, dengue fever epidemics have frequently outbreaks in more than 20 countries and regions around the world, wherein the Brazilian epidemics in the southeast Asia countries such as Philippines, Vietnam, Thailand, Malaysia and the south America are the most serious; the total number of dengue cases is over 150 thousands worldwide, causing widespread concern for people around the world.
Chinese herbs have been used for thousands of years in China. The traditional Chinese medicine is mainly based on various chemical components in the plant preparation, and the traditional Chinese medicine can interact and simultaneously play a role through various molecular targets and cell mechanisms. According to statistics, more than 80% of urban residents purchase Chinese patent medicines by themselves in China, and thus the Chinese patent medicines have huge domestic demand.
Although some studies on botanical drugs that may have effects of controlling dengue viruses have been conducted by researchers, the botanical drugs that are currently studied for controlling dengue viruses have short drug action time and poor efficacy.
Disclosure of Invention
The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating dengue fever virus, and aims to solve the problems of short drug effect time and poor curative effect of the existing botanical drugs for preventing and treating dengue fever virus.
The embodiment of the invention is realized in such a way that the traditional Chinese medicine composition for preventing and treating dengue fever virus comprises the following components in parts by weight: 18-30 parts of sweet wormwood herb extract, 20-33 parts of perilla extract and 7-11 parts of trogopterus dung.
The embodiment of the invention also provides a preparation method of the traditional Chinese medicine composition for preventing and treating dengue fever virus, which comprises the following steps: weighing the components according to the formula of the traditional Chinese medicine composition for preventing and treating the dengue fever virus for later use; adding 90-95% ethanol in an amount which is 13-14 times the mass of the trogopterus dung, heating, refluxing, extracting, cooling to room temperature, filtering, collecting an ethanol extract, concentrating to 10-12% of the original volume, and performing spray drying to obtain a trogopterus dung extract; mixing the sweet wormwood herb extract, the perilla herb extract and the trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating the dengue fever virus.
The traditional Chinese medicine composition for preventing and treating dengue fever virus provided by the embodiment of the invention adopts the traditional Chinese medicine raw materials of sweet wormwood herb extract, perilla herb extract and trogopterus dung which are plant sources for compatibility, has good prevention and treatment effects on dengue fever, dengue hemorrhagic fever and dengue shock syndrome caused by dengue virus infection, and has long drug effect time and good curative effect.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The traditional Chinese medicine composition for preventing and treating dengue fever virus provided by the embodiment of the invention adopts the traditional Chinese medicine raw materials of sweet wormwood herb extract, perilla herb extract and trogopterus dung which are plant sources for compatibility, has good prevention and treatment effects on dengue fever, dengue hemorrhagic fever and dengue shock syndrome caused by dengue virus infection, and has long drug effect time and good curative effect.
The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating dengue fever virus, which comprises the following components in parts by weight: 18-30 parts of sweet wormwood herb extract, 20-33 parts of perilla extract and 7-11 parts of trogopterus dung.
The traditional Chinese medicine composition for preventing and treating dengue fever virus provided by the embodiment of the invention is prepared from sweet wormwood herb extract, perilla extract and trogopterus dung as raw materials. If necessary, the traditional Chinese medicine composition of the invention can comprise other traditional Chinese medicines (or traditional Chinese medicine extracts).
For example, the Chinese medicinal composition for preventing and treating dengue fever virus provided by the embodiment of the invention has excellent antipyretic effect after cortex lycii radicis is additionally added, and the antipyretic effect is beneficial to enhancing the resistance of a patient to the virus, so that the prevention and treatment effect of the Chinese medicinal composition for preventing and treating dengue fever virus is further enhanced.
In the embodiment of the invention, the sweet wormwood herb extract is prepared by the following steps:
grinding and crushing whole fresh or dry sweet wormwood herb, adding an alkaline soak solution with the mass being 2-3 times of that of the fresh or dry sweet wormwood herb, soaking for 5-6 hours, pulping, standing and filtering to obtain sweet wormwood herb filtrate; and carrying out evaporative crystallization on the sweet wormwood herb filtrate, wherein the evaporative crystallization temperature is 30-40 ℃, and obtaining the sweet wormwood herb extract.
In the embodiment of the invention, the alkaline soaking solution is a sodium carbonate solution with the concentration of 0.3-0.4%. The sodium carbonate solution can effectively remove impurities and pesticide residues on the surface of the sweet wormwood herb, ensures the sanitation and safety of the sweet wormwood herb extract, is beneficial to quickly extracting effective substances (such as artemisinin) in the sweet wormwood herb, and improves the extraction efficiency of the sweet wormwood herb.
In the embodiment of the invention, the perilla extract is prepared by the following steps:
drying the whole perilla herb or fresh perilla leaf at 50-60 ℃ for 36-48 hours, taking out and crushing to obtain crushed perilla; performing ultrasonic extraction by using water as an extractant, wherein the ultrasonic power is 360-500 w, the weight ratio of the crushed perilla to the water is 1: 10-15, the temperature is 85-90 ℃, the time is 90-120 min, filtering the crushed perilla by using a diatomite filter, and then performing reduced pressure concentration at the temperature of below 60 ℃ until the relative density is 1-1.15 to obtain a perilla extract concentrated solution; adjusting the ethanol concentration of the concentrated perilla extract liquid with 70% ethanol to ensure that the ethanol volume is 6-7% to obtain the perilla extract liquid.
In a preferred embodiment of the present invention, the step of preparing the concentrated perilla extract solution specifically comprises: performing ultrasonic extraction with water as extractant at ultrasonic power of 450w, the weight ratio of the crushed perilla to water is 1:12, the temperature is 90 ℃, the time is 100min, filtering with a diatomite filter, and concentrating under reduced pressure at below 60 ℃ until the relative density is 1-1.15 to obtain the concentrated perilla extract.
In the embodiment of the invention, the perilla extract containing a small amount of ethanol is obtained by drying, crushing, ultrasonically extracting, decompressing and concentrating the whole perilla or fresh perilla leaves and adding ethanol for blending. The contents of brass, polyphenol and rosmarinic acid in the perilla extract extracted by the extraction method are higher than those of common direct pulping and filtering extraction.
Preferably, the traditional Chinese medicine composition for preventing and treating dengue fever virus comprises the following components in parts by weight: 18-25 parts of sweet wormwood herb extract, 20-27 parts of perilla extract and 10-11 parts of trogopterus dung. More preferably, the sweet wormwood herb extract is 19 parts, the perilla herb extract is 25 parts, and the trogopterus dung is 11 parts.
The Chinese medicinal composition of the present invention as a pharmaceutically active substance may be optionally mixed or combined with inorganic or organic, solid or liquid pharmaceutically acceptable carriers or adjuvants suitable for administration in order to be formulated into a suitable dosage form. Suitable carriers include, for example, fillers such as sugars (e.g. lactose), mannitol or sorbitol, cellulosics and/or calcium phosphates (tricalcium phosphate or calcium hydrogen phosphate); binders such as starch paste, gelatin, methylcellulose and/or polyvinyl pyrrolidone; disintegrating agent such as starch, carboxymethyl starch, cross-linked polyvinylpyrrolidone, agar or alginic acid or its salt (such as sodium alginate).
Preferably, the preparation taking the traditional Chinese medicine composition for preventing and treating dengue fever virus provided by the embodiment of the invention as an active substance is a tablet, an oral liquid or a capsule preparation.
The embodiment of the invention also provides a preparation method of the traditional Chinese medicine composition for preventing and treating dengue fever virus, which comprises the following steps:
step 101, weighing the components according to the formula of the traditional Chinese medicine composition for preventing and treating dengue fever virus for later use.
102, adding 90-95% ethanol in an amount which is 13-14 times the mass of the trogopterus dung, heating, refluxing, extracting, cooling to room temperature, filtering, collecting an ethanol extract, concentrating to 10-12% of the original volume, and performing spray drying to obtain the trogopterus dung extract.
103, mixing the sweet wormwood herb extract, the perilla herb extract and the trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating the dengue fever virus.
In step 102, preferably adding 90% ethanol in an amount 13 times the mass of trogopterus dung, heating, refluxing, extracting, cooling to room temperature, filtering, collecting ethanol extractive solution, and concentrating to 10% of original volume; spray drying to obtain Oletum Trogopterori extract.
The trogopterus dung which is subjected to the specific treatment is used together with the sweet wormwood herb extract and the perilla herb extract, so that the prevention and treatment effects of the traditional Chinese medicine composition on dengue fever, dengue hemorrhagic fever and dengue shock syndrome caused by dengue fever virus infection are well improved.
Examples of certain embodiments of the invention are given below, which are not intended to limit the scope of the invention.
In addition, it should be noted that the numerical values given in the following examples are as precise as possible, but those skilled in the art will understand that each numerical value should be understood as a divisor rather than an absolutely exact numerical value due to measurement errors and experimental operational problems that cannot be avoided. For example, due to errors in the weighing apparatus, it should be understood that the weight values for each drug in the compositions of the examples may have errors of ± 2% or ± 1%.
Example 1
The formulation of the traditional Chinese medicine composition for preventing and treating dengue fever virus in example 1 is as follows:
180g of sweet wormwood herb extract, 300g of perilla extract and 70g of trogopterus dung.
The preparation process of the traditional Chinese medicine composition for preventing and treating dengue fever virus of the embodiment 1 is as follows:
grinding and crushing the fresh sweet wormwood herb, adding a sodium carbonate solution with the mass of 2 times and the concentration of 0.3% of the fresh sweet wormwood herb, soaking for 5 hours, pulping, standing and filtering to obtain sweet wormwood herb filtrate; then carrying out evaporation crystallization on the sweet wormwood herb filtrate at the temperature of 30 ℃ to obtain sweet wormwood herb extract;
drying whole herb of Perillae herba at 50 deg.C for 36 hr, taking out, and pulverizing to obtain Perillae herba pulverized material; performing ultrasonic extraction with water as extractant at ultrasonic power of 360w, the weight ratio of the crushed Perillae herba and water is 1:10, the temperature is 85 deg.C, the time is 90min, filtering with diatomite filter, and concentrating under reduced pressure at below 60 deg.C to relative density of 1 to obtain concentrated Perillae herba extract solution; adjusting the ethanol concentration of the concentrated solution of the perilla extract with 70% ethanol to make the ethanol volume be 6% to obtain a perilla extract;
adding 90% ethanol 13 times the weight of Oletum Trogopterori, heating, reflux-extracting, cooling to room temperature, filtering, collecting ethanol extractive solution, concentrating to 10% of the original volume, and spray drying to obtain Oletum Trogopterori extract;
and mixing 180g of the prepared sweet wormwood herb extract, 300g of the perilla herb extract and 70g of the trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating the dengue fever virus.
Example 2
The formulation of the traditional Chinese medicine composition for preventing and treating dengue fever virus in example 2 is as follows:
200g of sweet wormwood herb extract, 200g of perilla extract and 110g of trogopterus dung;
the preparation process of the traditional Chinese medicine composition for preventing and treating dengue fever virus of the embodiment 2 is as follows:
grinding and crushing a fresh sweet wormwood herb, adding a sodium carbonate solution with the mass of 3 times and the concentration of 0.4% of the fresh sweet wormwood herb, soaking for 6 hours, pulping, standing and filtering to obtain sweet wormwood herb filtrate; evaporating and crystallizing the sweet wormwood filtrate at 40 deg.C to obtain sweet wormwood extract;
drying the whole herb of Perilla frutescens at 50 deg.C for 48 hr, taking out, and pulverizing to obtain Perilla frutescens pulverized material; performing ultrasonic extraction with water as extractant at ultrasonic power of 400w, the weight ratio of the crushed Perillae herba and water is 1:11, the temperature is 85 deg.C, the time is 100min, filtering with diatomite filter, and concentrating under reduced pressure at below 60 deg.C to relative density of 1.1 to obtain concentrated Perillae herba extract solution; adjusting the ethanol concentration of the concentrated solution of Perillae herba extract with 70% ethanol to make the ethanol volume be 6.5% to obtain Perillae herba extract;
adding ethanol with the mass concentration of 95% and the mass of 14 times of that of the trogopterus dung into the trogopterus dung, heating, refluxing, extracting, cooling to room temperature, filtering, collecting ethanol extract, concentrating to 11% of the original volume, and spray drying to obtain a trogopterus dung extract;
mixing 200g of the prepared sweet wormwood herb extract, 200g of perilla extract and 110g of trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating dengue fever virus.
Example 3
The formulation of the traditional Chinese medicine composition for preventing and treating dengue fever virus in example 3 is as follows:
250g of sweet wormwood herb extract, 330g of perilla extract and 80g of trogopterus dung;
the preparation process of the traditional Chinese medicine composition for preventing and treating dengue fever virus of the embodiment 3 is as follows:
grinding and crushing the fresh sweet wormwood herb, adding a sodium carbonate solution with the mass of 2.5 times and the concentration of 0.4 percent of the fresh sweet wormwood herb, soaking for 6 hours, pulping, standing and filtering to obtain sweet wormwood herb filtrate; evaporating and crystallizing the sweet wormwood filtrate at 35 deg.C to obtain sweet wormwood extract;
drying the whole herb of Perilla frutescens at 55 deg.C for 48 hr, taking out, and pulverizing to obtain Perilla frutescens pulverized material; performing ultrasonic extraction with water as extractant at ultrasonic power of 500w, the weight ratio of the crushed Perillae herba and water is 1:15, the temperature is 90 deg.C, the time is 100min, filtering with diatomite filter, and concentrating under reduced pressure at below 60 deg.C to relative density of 1.15 to obtain Perillae herba extract concentrated solution; adjusting the ethanol concentration of the concentrated solution of the perilla extract with 70% ethanol to make the ethanol volume be 7% to obtain a perilla extract;
adding 90% ethanol 13 times the weight of Oletum Trogopterori, heating, reflux-extracting, cooling to room temperature, filtering, collecting ethanol extractive solution, concentrating to 12% of the original volume, and spray drying to obtain Oletum Trogopterori extract;
and mixing 250g of the prepared sweet wormwood herb extract, 330g of perilla extract and 80g of trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating the dengue fever virus.
Example 4
The formulation of the traditional Chinese medicine composition for preventing and treating dengue fever virus in example 4 is as follows:
300g of sweet wormwood herb extract, 270g of perilla extract and 90g of trogopterus dung;
the preparation process of the traditional Chinese medicine composition for preventing and treating dengue fever virus of the embodiment 4 is as follows:
grinding and crushing the whole sweet wormwood herb dry product, adding a sodium carbonate solution with the mass of 3 times and the concentration of 0.4% of the sweet wormwood herb dry product, soaking for 5 hours, pulping, standing and filtering to obtain sweet wormwood herb filtrate; evaporating and crystallizing the sweet wormwood filtrate at 35 deg.C to obtain sweet wormwood extract;
drying whole plant of Perillae herba at 60 deg.C for 36 hr, and pulverizing to obtain Perillae herba pulverized material; performing ultrasonic extraction with water as extractant at ultrasonic power of 450w, the weight ratio of the crushed Perillae herba and water is 1:14, the temperature is 90 deg.C, the time is 120min, filtering with diatomite filter, and concentrating under reduced pressure at below 60 deg.C to relative density of 1.15 to obtain concentrated Perillae herba extract solution; adjusting the ethanol concentration of the concentrated solution of the perilla extract with 70% ethanol to make the ethanol volume be 7% to obtain a perilla extract;
adding 90% ethanol 13 times the weight of Oletum Trogopterori, heating, reflux-extracting, cooling to room temperature, filtering, collecting ethanol extractive solution, concentrating to 10% of the original volume, and spray drying to obtain Oletum Trogopterori extract;
and mixing 300g of the prepared sweet wormwood herb extract, 270g of the perilla herb extract and 90g of the trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating the dengue fever virus.
Example 5
The formulation of the traditional Chinese medicine composition for preventing and treating dengue fever virus in example 5 is as follows:
190g of sweet wormwood herb extract, 250g of perilla extract and 110g of trogopterus dung.
The preparation process of the traditional Chinese medicine composition for preventing and treating dengue fever virus of the embodiment 5 is as follows:
grinding and crushing a fresh sweet wormwood herb, adding a sodium carbonate solution with the mass of 3 times and the concentration of 0.4% of the fresh sweet wormwood herb, soaking for 6 hours, pulping, standing and filtering to obtain sweet wormwood herb filtrate; evaporating and crystallizing the sweet wormwood filtrate at 35 deg.C to obtain sweet wormwood extract;
drying the whole herb of Perilla frutescens at 55 deg.C for 48 hr, taking out, and pulverizing to obtain Perilla frutescens pulverized material; performing ultrasonic extraction with water as extractant at ultrasonic power of 450w, the weight ratio of the crushed Perillae herba and water is 1:11, the temperature is 90 deg.C, the time is 110min, filtering with diatomite filter, and concentrating under reduced pressure at below 60 deg.C to relative density of 1.1 to obtain Perillae herba extract concentrated solution; adjusting the ethanol concentration of the concentrated solution of the perilla extract with 70% ethanol to make the ethanol volume be 6% to obtain a perilla extract;
adding 90% ethanol 13 times the weight of Oletum Trogopterori, heating, reflux-extracting, cooling to room temperature, filtering, collecting ethanol extractive solution, concentrating to 10% of the original volume, and spray drying to obtain Oletum Trogopterori extract;
and mixing 190g of the prepared sweet wormwood herb extract, 250g of perilla extract and 110g of trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating the dengue fever virus.
Comparative example 1
Compared with example 5, the traditional Chinese medicine composition for preventing and treating dengue fever virus provided in comparative example 1 has the same raw materials and preparation method as example 5 except that the artemisia annua extract and the content thereof are omitted.
Comparative example 2
Compared with example 5, the traditional Chinese medicine composition for preventing and treating dengue fever virus provided in comparative example 2 is identical to example 5 in all raw materials and preparation method except that the perilla herb extract and the content thereof are omitted.
Comparative example 3
Compared with example 5, the traditional Chinese medicine composition for preventing and treating dengue fever virus provided in comparative example 3 is the same as example 5 except that trogopterus dung and the content thereof are omitted.
Comparative example 4
Compared with example 5, the artemisia apiacea extract in the traditional Chinese medicine composition for controlling dengue fever virus provided by comparative example 4 is prepared by the following steps: grinding fresh herba Artemisiae Annuae, pulping, filtering, and evaporating at 35 deg.C to obtain herba Artemisiae Annuae extract. The remaining raw materials and the preparation method were the same as in example 5.
Comparative example 5
Compared with example 5, the perilla extract liquid in the traditional Chinese medicine composition for preventing and treating dengue fever virus provided by comparative example 5 is prepared by the following steps: pulping whole perilla, filtering, performing ultrasonic extraction with ultrasonic power of 450w, and filtering with diatomite filter at 90 deg.C for 110min, wherein the weight ratio of crushed perilla to water is 1: 11. The remaining raw materials and the preparation method were the same as in example 5.
Comparative example 6
Compared with the example 5, in the comparative example 6, when the traditional Chinese medicine composition for preventing and treating dengue fever virus is prepared, trogopterus dung is directly pulverized and added for use, and the rest raw materials and the preparation method are the same as those in the example 5;
first, cytotoxicity test
1. Test samples: the traditional Chinese medicine composition for preventing and treating dengue fever virus provided by the embodiment 5 of the invention.
2. Test methods and test results: preparing a single cell suspension by using a DMEM medium containing 5% fetal bovine serum, and inoculating 2000-3000A 549 cells in each hole to a 96-hole cell culture plate, wherein the inoculation volume of each hole is 100 uL; at 37 ℃ 5% CO2Culturing for 1 day under the culture condition; the DMEM medium in each well was aspirated and 100uL of the Chinese medicinal composition of example 5 diluted to the corresponding concentration (10UM, 25UM, 50UM) in DMEM medium containing 5% fetal bovine serum was added to each well, and 100uL of DMEM medium containing 5% fetal bovine serum without drug was added to the control wells; after 24 hours of incubation, 10uL of MTT solution was added to each well at 37 ℃ in 5% CO2Continuously incubating for 4 hours under the culture condition, then terminating the culture, removing culture supernatant in each hole by suction, adding 150uL DMSO in each hole, and oscillating for 10min to fully melt crystals; the 490nm wavelength was selected, the absorbance of each well was measured on an enzyme linked immunosorbent assay, and the results are reported in Table 1.
As can be seen from the above tests, the survival rates of a549 cells at the concentrations of the Chinese medicinal composition provided in examples 5 of 0UM, 10UM, 25UM and 50UM were 100%, 96.7%, 95.3% and 92.4%, respectively.
The cytotoxicity test of the traditional Chinese medicine composition prepared in the embodiments 1 to 4 of the invention is carried out by adopting the test method, and the test results are shown in the following table 2.
From the test results in table 2 above, the survival rates of a549 cells at the concentrations of the traditional Chinese medicine compositions provided in examples 1 to 4 of 0UM, 10UM, 25UM and 50UM are respectively 100%, 94.5% to 96.3%, 93.7% to 95% and 91.3% to 92.1%.
Second, inhibition of dengue II virus
DENV2 MOI =1 infected a549 cells, experimental procedure as follows:
1. planting cells: a549 cells are inoculated in a 24-hole cell culture plate, after 24 hours, the cells grow to a monolayer (the area of the cell covering the bottom of a hole is about 80-90%), a culture medium is sucked out, a virus sample is inoculated in 100uL, and the virus sample is adsorbed at 37 ℃ for 1 hour. After completion of the adsorption, the virus solution in each well was aspirated, and the unadsorbed virus was washed off with DMEM medium. The Chinese medicinal compositions of examples 1 to 5 and comparative examples 1 to 6 were added at a given concentration (10UM) diluted with DMEM medium containing 5% fetal bovine serum, and DMSO was added to the control well at the same volume at 37 ℃ and 5% CO2Was cultured in the incubator of (1) for 24 hours, and the supernatant from each well was collected and then the virus titer was measured according to TCID50, the results of which are shown in Table 3 below.
2. TCID50 experiments:
determination of the minimum viral load causing 50% of the lesions after incubation of dengue type II virus infected susceptible cells C6/36, namely: c6/36 cells are inoculated in a 96-well plate → 24h, and then 100uL dengue fever type II virus diluted by 10 times in a culture medium containing 2% FBS is sequentially inoculated, the dilution is 10-1 to 0-10, each dilution has 6 auxiliary wells → is placed in a 37-welloC，5％CO2Adsorbing for 1 hr in incubator, adding 100uL of 2% FBS-containing medium at 35 deg.C and 5% CO2And (5) culturing in an incubator, observing and recording results after about 5-6 days, wherein the results are shown in the following table 4.
As shown in the test results of tables 2 to 4 above, the traditional Chinese medicine composition for preventing and treating dengue fever virus provided by embodiments 1 to 5 of the invention has low toxicity and high efficiency, can effectively inhibit dengue fever type II virus, and has an inhibition effect obviously superior to that of comparative examples 1 to 6. This indicates that the inhibition effect of the extract of Artemisia annua, the extract of Perilla frutescens and Oletum Trogopterori (extract) is better than that of the extract of Artemisia annua, the extract of Perilla frutescens and Oletum Trogopterori (extract) which are used alone. And when preparing the sweet wormwood extract, the sweet wormwood extract is prepared by the process of soaking the sweet wormwood extract by a sodium carbonate solution and then pulping and extracting; when preparing the perilla extracting solution, concentrating the filtered perilla filtrate, and adding ethanol for adjustment to obtain the perilla extracting solution; and the trogopterus dung extract prepared by the alcohol extraction process is beneficial to improving the inhibiting effect of the composition on dengue fever II viruses.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. The traditional Chinese medicine composition for preventing and treating dengue fever virus is characterized by comprising the following components in parts by weight:
18-30 parts of sweet wormwood herb extract, 20-33 parts of perilla extract and 7-11 parts of trogopterus dung.
2. The Chinese medicinal composition for preventing and treating dengue fever virus according to claim 1, wherein the artemisia apiacea extract is prepared by the following steps:
grinding and crushing whole fresh or dry sweet wormwood herb, adding an alkaline soak solution with the mass being 2-3 times of that of the fresh or dry sweet wormwood herb, soaking for 5-6 hours, pulping, standing and filtering to obtain sweet wormwood herb filtrate;
and carrying out evaporative crystallization on the sweet wormwood herb filtrate, wherein the evaporative crystallization temperature is 30-40 ℃, and obtaining the sweet wormwood herb extract.
3. The traditional Chinese medicine composition for preventing and treating dengue fever virus according to claim 2, wherein the alkaline soak solution is a sodium carbonate solution with a concentration of 0.3-0.4%.
4. The traditional Chinese medicine composition for preventing and treating dengue fever virus according to claim 1, wherein the perilla extract is prepared by the following steps:
drying the whole perilla herb or fresh perilla leaf at 50-60 ℃ for 36-48 hours, taking out and crushing to obtain crushed perilla;
performing ultrasonic extraction by using water as an extractant, wherein the ultrasonic power is 360-500 w, the weight ratio of the crushed perilla to the water is 1: 10-15, the temperature is 85-90 ℃, the time is 90-120 min, filtering the crushed perilla by using a diatomite filter, and then performing reduced pressure concentration at the temperature of below 60 ℃ until the relative density is 1-1.15 to obtain a perilla extract concentrated solution;
adjusting the ethanol concentration of the concentrated perilla extract liquid with 70% ethanol to ensure that the ethanol volume is 6-7% to obtain the perilla extract liquid.
5. The traditional Chinese medicine composition for preventing and treating dengue fever virus as claimed in claim 4, wherein the ultrasonic extraction is carried out by using water as an extractant, the ultrasonic power is 360-500 w, the weight ratio of the crushed perilla to water is 1: 10-15, the temperature is 85-90 ℃, the time is 90-120 min, the crushed perilla is filtered by a diatomite filter and then is concentrated under reduced pressure at the temperature below 60 ℃ until the relative density is 1-1.15, and the step of obtaining the concentrated perilla extract solution specifically comprises the following steps:
performing ultrasonic extraction with water as extractant at ultrasonic power of 450w, the weight ratio of the crushed perilla to water is 1:12, the temperature is 90 ℃, the time is 100min, filtering with a diatomite filter, and concentrating under reduced pressure at below 60 ℃ until the relative density is 1-1.15 to obtain the concentrated perilla extract.
6. The traditional Chinese medicine composition for preventing and treating dengue fever virus according to claim 1, which comprises the following components in parts by weight:
18-25 parts of sweet wormwood herb extract, 20-27 parts of perilla extract and 10-11 parts of trogopterus dung.
7. The traditional Chinese medicine composition for preventing and treating dengue fever virus according to claim 6, which comprises the following components in parts by weight:
19 parts of sweet wormwood herb extract, 25 parts of perilla extract and 11 parts of trogopterus dung.
8. The traditional Chinese medicine composition for preventing and treating dengue fever virus according to claim 1, wherein the traditional Chinese medicine composition for preventing and treating dengue fever virus is prepared into a tablet, an oral liquid or a capsule preparation.
9. The method for preparing the Chinese medicinal composition for preventing and treating dengue fever virus according to any one of claims 1 to 8, which comprises the following steps:
weighing the components according to the formula of the traditional Chinese medicine composition for preventing and treating dengue fever virus as claimed in any one of claims 1 to 8 for later use;
adding 90-95% ethanol in an amount which is 13-14 times the mass of the trogopterus dung, heating, refluxing, extracting, cooling to room temperature, filtering, collecting an ethanol extract, concentrating to 10-12% of the original volume, and performing spray drying to obtain a trogopterus dung extract;
mixing the sweet wormwood herb extract, the perilla herb extract and the trogopterus dung extract to obtain the traditional Chinese medicine composition for preventing and treating the dengue fever virus.
10. The method for preparing a Chinese medicinal composition for preventing and treating dengue fever virus according to claim 9, wherein the step of adding ethanol with a mass concentration of 90-95% in an amount 13-14 times the mass of trogopterus dung into the trogopterus dung, heating and refluxing for extraction, cooling to room temperature, filtering, collecting an ethanol extract, concentrating to 10-12% of the original volume, and spray-drying to obtain the trogopterus dung extract comprises the following steps:
adding 90% ethanol 13 times the weight of Oletum Trogopterori, heating, reflux extracting, cooling to room temperature, filtering, collecting ethanol extractive solution, and concentrating to 10% of original volume; spray drying to obtain Oletum Trogopterori extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010763694.XA CN111743935A (en) | 2020-08-01 | 2020-08-01 | Traditional Chinese medicine composition for preventing and treating dengue fever virus and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010763694.XA CN111743935A (en) | 2020-08-01 | 2020-08-01 | Traditional Chinese medicine composition for preventing and treating dengue fever virus and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111743935A true CN111743935A (en) | 2020-10-09 |
Family
ID=72712646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010763694.XA Pending CN111743935A (en) | 2020-08-01 | 2020-08-01 | Traditional Chinese medicine composition for preventing and treating dengue fever virus and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111743935A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114632453A (en) * | 2022-05-18 | 2022-06-17 | 广东卫康有害生物防制有限公司 | Multiphase convection blending device and preparation method of mosquito-killing composition for preventing and controlling dengue fever virus |
-
2020
- 2020-08-01 CN CN202010763694.XA patent/CN111743935A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 李开国等: "青蒿煎剂治疗登革热疗效观察", 《中草药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114632453A (en) * | 2022-05-18 | 2022-06-17 | 广东卫康有害生物防制有限公司 | Multiphase convection blending device and preparation method of mosquito-killing composition for preventing and controlling dengue fever virus |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111870657B (en) | Application of traditional Chinese medicine composition in preparation of medicine for treating or preventing coronavirus infection | |
| CN105770207B (en) | Viola yedoensis makino extract and application thereof | |
| US20230158095A1 (en) | Application of traditional chinese medicine composition to preparation of medicine for treating or preventing coronavirus infection | |
| JP2022545288A (en) | Tea composition for prevention, improvement or effect of respiratory disease and pharmaceutical composition containing the same | |
| CN108524685B (en) | Composition for treating children herpangina and application thereof | |
| CN112691138B (en) | Preparation method of mulberry resource extract with in-vitro inhibitory activity on African swine fever virus | |
| CN111743935A (en) | Traditional Chinese medicine composition for preventing and treating dengue fever virus and preparation method thereof | |
| WO2021218422A1 (en) | Application of traditional chinese medicine composition in preparation of drugs for treating or preventing coronavirus infection | |
| US20030054047A1 (en) | Pharmaceutical composition for the treatment of viral infection | |
| CN108403858B (en) | Callicarpa nudiflora extract composition for treating hand-foot-and-mouth disease and application thereof | |
| CN108186866B (en) | Application of golden scallop oral liquid in antivirus | |
| CN115105502B (en) | Application of compound containing stephania plant alkaloid in preparation of cat infectious peritonitis medicine | |
| CN114668792A (en) | Extraction method and application of paper mulberry extract | |
| CN113616764B (en) | Antiviral traditional Chinese medicine composition and application thereof | |
| CN110201068B (en) | Application of Kegan Liyan composition or preparation in preparation of medicine for preventing and treating dengue fever | |
| CN107281235B (en) | The purposes of wintersweet platymiscium resisiting influenza virus | |
| CN113288943A (en) | Application of traditional Chinese medicine composition in preparation of medicine for treating or preventing coronavirus infection | |
| CN115141681B (en) | Volatile oil, preparation method and application thereof | |
| CN113456767B (en) | Anti-coronavirus traditional Chinese medicine composition and application thereof | |
| CN113244276B (en) | Use of Sang Huanghuo Phellinus linteus extract as novel coronavirus therapeutic drug or antiviral agent | |
| CN113230289A (en) | Application of phellinus igniarius and thesium Chinese compound serving as novel coronavirus therapeutic drug or antiviral preparation | |
| CN112641867B (en) | Antiviral traditional Chinese medicine composition and application thereof | |
| CN105816522A (en) | Traditional Chinese medicine composition for resisting respiratory syncytial virus and preparation method thereof | |
| CN107115370B (en) | The purposes of the anti-enterovirus of wintersweet platymiscium | |
| CN113230288A (en) | Application of thesium or thesium extract as novel coronavirus therapeutic drug or antiviral preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201009 |