CN111733106B - Culture method and application of staphylococcus squirrel F-E8-1 - Google Patents

Culture method and application of staphylococcus squirrel F-E8-1 Download PDF

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CN111733106B
CN111733106B CN202010638407.2A CN202010638407A CN111733106B CN 111733106 B CN111733106 B CN 111733106B CN 202010638407 A CN202010638407 A CN 202010638407A CN 111733106 B CN111733106 B CN 111733106B
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staphylococcus
squirrel
wastewater
sciuri
salt
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CN111733106A (en
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赵晨
岳秋林
赵林
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Shandong Zhuoran Biotechnology Co ltd
Qilu University of Technology
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Shandong Zhuoran Biotechnology Co ltd
Qilu University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/44—Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
    • C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds

Abstract

The invention belongs to the technical field of microbial culture, and particularly relates to a culture method of staphylococcus squirrel capable of tolerating a high-salt environment, and an application of the staphylococcus squirrel in treatment of fermentation production wastewater. Staphylococcus squirrel (capable of tolerating high salt environment) ((Staphylococcus sciuri) F-E8-1, the application of the strain in the rapid degradation of residual sugar in organic acid and/or amino acid fermentation production wastewater in a high-salt environment, is the important protection scope of the invention. The method treats the specific fermentation production wastewater through the microbial strains, and has the remarkable advantages of low operation cost, less consumption, high efficiency, strong industrial adaptability, stable operation, convenient operation and management and the like; the staphylococcus squirrel provided by the invention has excellent high-salt resistance, and has a good treatment effect in the process of treating wastewater water with high salt content; the strain disclosed by the invention is used for treating high-salinity wastewater, the time consumption is short, and the treatment effect is good.

Description

Culture method and application of staphylococcus squirrel F-E8-1
Technical Field
The invention belongs to the technical field of microbial culture, and particularly relates to a culture method of staphylococcus squirrel capable of tolerating high-salt environment, and the staphylococcus squirrelStaphylococcus sciuriThe application of F-E8-1 in treating fermentation production wastewater.
Background
Regarding staphylococcus squirrel, CN103865859A discloses a strain of staphylococcus squirrel, vaccine and preparation method thereof, wherein the staphylococcus squirrel is mainly used as inactivated vaccine;
the following disclosures have been made in the journal literature for staphylococcus squirrel: in the text of research on bacteriostasis of composite biological preservative on Staphylococcus squirrel and mechanism of action thereof, bluegrass et al disclose that gram-positive dominant bacteria, Staphylococcus squirrel (Staphylococcus sciuri), separated from refrigerated hairtail are used as test bacteria to research the bacteriostasis effect and mechanism of action of composite biological preservative (the mixture ratio concentration is 10.0 g/L of chitosan, 0.3 g/L of lysozyme and 3.0 g/L of tea polyphenol) on Staphylococcus squirrel.
There is currently little disclosure of methods for the culture of staphylococcus pini strains, and methods for their ability to act on and effectively treat wastewater, particularly high-salt fermentation wastewater.
Regarding the high-salt fermentation wastewater, CN109136095A discloses a wastewater from monosodium glutamate fermentation production, which uses three microorganisms to effectively utilize the wastewater to prepare microbial protein and purify the wastewater, but the above patents do not refer to whether the three microorganisms have high salt tolerance and have good ability to purify the wastewater in high-salt environment. In fact, in the wastewater from the fermentation production of organic acids and amino acids, the salt content is very high, and if the wastewater is treated by microorganisms, the microorganisms must have excellent salt tolerance.
Disclosure of Invention
In order to solve the problems, the invention provides a salt-tolerant efficient degradation bacterium staphylococcus squirrel (Staphylococcus sciuri) The strain obtained by the culture method can rapidly degrade residual sugar in organic acid and amino acid fermentation production wastewater in a high-salt environment, and is short in time consumption, good in effect and low in treatment cost when used for treating high-salt wastewater. After solid-liquid separation and thallus removal, crude salt contained in the fermentation wastewater can be concentrated and recovered.
According to the invention, the staphylococcus squirrel which is resistant to high-salt and efficient degradation is added into the high-salt fermentation wastewater treatment system, so that the degradation of the microorganisms to pollutants is accelerated, the biological treatment efficiency of the system is improved, the purpose of rapidly degrading residual sugar in the fermentation wastewater is achieved, and after thalli are removed through solid-liquid separation, crude salt contained in the fermentation wastewater is concentrated and recovered.
The invention provides a ring capable of tolerating high saltEnvironmental Staphylococcus squirrel: (Staphylococcus sciuri) F-E8-1, wherein the strain is preserved in China Center for Type Culture Collection (CCTCC) in 3/6/2020, and the preservation number is M2020052, and the preservation address is as follows: wuhan university Collection No. 202, Wuhan City, Hubei province, China.
Staphylococcus saimiriStaphylococcus sciuriF-E8-1 was obtained by culturing as follows: staphylococcus squirrel on LB plateStaphylococcus sciuriF-E8-1 single colony is inoculated in a seed culture medium, cultured for 12-18 h at 37-42 ℃, then inoculated into a fermentation culture medium according to the inoculum size of 4-6%, and cultured for 96-120 h at 37-42 ℃.
Staphylococcus saimiriStaphylococcus sciuriThe seed culture medium of F-E8-1 comprises the following raw materials in percentage by weight: 0.35-0.65% of yeast powder, 0.8-1.2% of peptone and 0.35-0.65% of sodium chloride.
Staphylococcus saimiriStaphylococcus sciuriThe fermentation medium of F-E8-1 comprises the following raw materials in percentage by weight: glutamic acid waste liquid containing 20-30 wt% of ammonium sulfate and the balance of sterile water, and adjusting the pH value to 7.0-7.5 by adopting 1 MNaOH.
Staphylococcus saimiriStaphylococcus sciuriAfter the F-E8-1 is cultured in a fermentation medium, the residual sugar in the wastewater is reduced to 0.2-0.5%.
Staphylococcus squirrel cultured by the above methodStaphylococcus sciuriThe F-E8-1 strain has the following characteristics:
culturing in LB culture medium at 37 deg.C for 24 hr to obtain yellowish opaque single colony with wet and glossy surface and smooth edge and diameter of 3-4 mm; gram-positive G + cells are spherical and spore-free; the test is positive in oxidase test, positive in catalase test, positive in gelatin liquefaction test and negative in amylase test, the tests of glucose, maltose, sucrose, galactose and mannose are all positive, and the tests of mannitol, sorbitol, lactose, arabinose, arginine and xylose rhamnose are all negative.
The application of the strain in the rapid degradation of residual sugar in the wastewater produced by fermenting organic acid and/or amino acid in the high-salt environment is the important protection range of the invention.
In the application process, the amino nitrogen content of the high-salt fermentation wastewater is adjusted to 0.5-1.0%, the pH value is 3.0-9.0, the staphylococcus pini strain is inoculated into the wastewater according to the inoculation amount of 0.1-1.0%, and the fermentation wastewater is subjected to ventilation culture at 25-37 ℃ for 24-72 hours. The temperature, the inoculum size and the incubation time have a significant effect on the amount of degradation. The excessively low temperature greatly prolongs the fermentation time. Too long an inoculum size and culture time directly increases production costs. The amino nitrogen content and pH of the fermentation wastewater also influence the total amount of the bacteria. The fermentation time can be prolonged when the temperature is low, and the harmful effect can be generated on microorganisms when the temperature is too high, wherein the optimal fermentation temperature is 25-37 ℃; after the inoculation amount is increased to a certain degree, the influence on the degradation speed and the degradation effect of the system is not changed greatly. The optimal inoculation amount is between 0.1 and 1 percent.
The fermentation production wastewater is concentrated or unconcentrated wastewater comprehensively generated in each process link after fermentation of glutamic acid, threonine, tryptophan, lysine, citric acid and malic acid, and the salt content of the wastewater is 5-40%.
Culturing until the residual sugar in the wastewater is less than or equal to 0.2 percent, performing solid-liquid separation to remove thalli, and concentrating and recovering crude salt contained in the fermentation wastewater.
And performing solid-liquid separation by adopting any one of plate-frame filter pressing, disc centrifugation and tubular centrifugation.
When solid-liquid separation is carried out, the mesh number of the plate frame filter cloth is more than or equal to 200 meshes, and the centrifugal force of the centrifugal machine is more than or equal to 6000 g.
When the crude salt is recovered, multi-effect evaporation or forced circulation evaporation is adopted, and then fluidized bed drying is carried out, wherein the drying temperature is 60-120 ℃.
The invention has the beneficial effects that:
(1) the invention treats the specific fermentation production wastewater through the microbial strains, thereby avoiding the defects of long process flow, large occupied area, poor resource utilization and high treatment cost in physical and chemical treatment methods; the method has the remarkable advantages of low operation cost, less consumption, high efficiency, strong work adaptability, stable operation, convenient operation and management and the like;
(2) the staphylococcus squirrel provided by the invention has excellent high-salt resistance, and has a good treatment effect in the process of treating wastewater water with high salt content;
(3) the strain disclosed by the invention is used for treating high-salinity wastewater, so that the time consumption is short, and the treatment effect is good;
(4) after solid-liquid separation is carried out on the wastewater treated by the staphylococcus squirrel strain, thalli are removed, and crude salt contained in the fermentation wastewater can be concentrated and recovered.
Drawings
FIG. 1 is a microscopic examination picture of Staphylococcus aureus in squirrel;
FIG. 2 is a photograph of Staphylococcus pini colonies.
Detailed Description
The present invention will be further described with reference to specific examples so that those skilled in the art may better understand the present invention, but the present invention is not limited thereto.
Example 1A
Staphylococcus saimiri (A), (B), (C)Staphylococcus sciuri）F-E8-1, and the culture method is as follows:
staphylococcus squirrel on LB plate（Staphylococcus sciuri）F-E8-1 single colony is inoculated in a seed culture medium, cultured for 15h at 40 ℃, then inoculated into a fermentation culture medium according to the inoculum size of 5%, cultured for 110h at 40 ℃, and the residual sugar in the wastewater can be reduced to about 0.25%.
The seed culture medium comprises the following raw materials in percentage by weight: 0.45% of yeast powder, 0.9% of peptone and 0.55% of sodium chloride;
staphylococcus saimiri（Staphylococcus sciuri）The fermentation medium of F-E8-1 comprises the following raw materials in percentage by weight: the glutamic acid high-concentration waste liquid containing 25 wt% of ammonium sulfate and the balance of sterile water are adjusted to a pH value of about 7.0 by using 1M NaOH.
Example 1B
Staphylococcus saimiri (A), (B), (C)Staphylococcus sciuri）F-E8-1, and the culture method is as follows:
staphylococcus squirrel on LB plate（Staphylococcus sciuri）Seed culture medium inoculated with F-E8-1 single colonyCulturing at 37 deg.C for 18h, inoculating to fermentation medium at 6%, culturing at 37 deg.C for 120h, and reducing residual sugar to about 0.28%.
The seed culture medium comprises the following raw materials in percentage by weight: 0.35% of yeast powder, 1.2% of peptone and 0.35% of sodium chloride;
staphylococcus saimiri（Staphylococcus sciuri）The fermentation medium of F-E8-1 comprises the following raw materials in percentage by weight: the high-concentration waste liquor of glutamic acid containing 20% of ammonium sulfate and the balance of sterile water are adjusted to pH value of about 7.0 by 1M NaOH.
Example 1C
Staphylococcus saimiri (A), (B), (C)Staphylococcus sciuri）F-E8-1, and the culture method is as follows:
staphylococcus squirrel on LB plate（Staphylococcus sciuri）F-E8-1 single colony is inoculated in a seed culture medium, cultured for 12h at 42 ℃, inoculated into a fermentation culture medium according to the inoculum size of 4 percent, cultured for 96h at 42 ℃, and the residual sugar in the wastewater can be reduced to about 0.3 percent.
The seed culture medium comprises the following raw materials in percentage by weight: 0.65% of yeast powder, 0.8% of peptone and 0.65% of sodium chloride;
staphylococcus saimiri（Staphylococcus sciuri）The fermentation medium of F-E8-1 comprises the following raw materials in percentage by weight: the high-concentration waste liquor of glutamic acid containing 30% of ammonium sulfate and the balance of sterile water are adjusted to pH value of about 7.5 by 1M NaOH.
Example 1D
Staphylococcus saimiri (A), (B), (C)Staphylococcus sciuri）F-E8-1, and the culture method is as follows:
staphylococcus squirrel on LB plate（Staphylococcus sciuri）F-E8-1 single colony is inoculated in a seed culture medium, cultured for 16h at 38 ℃, then inoculated into a fermentation culture medium according to the inoculum size of 5%, cultured for 108h at 41 ℃, and the residual sugar in the wastewater can be reduced to about 0.3%.
The seed culture medium comprises the following raw materials in percentage by weight: 0.65% of yeast powder, 1% of peptone and 0.65% of sodium chloride;
squirrel grapeCoccus bacterium（Staphylococcus sciuri）The fermentation medium of F-E8-1 comprises the following raw materials in percentage by weight: the high-concentration waste liquor of glutamic acid containing 30% of ammonium sulfate and the balance of sterile water are adjusted to pH value of about 7.3 by 1M NaOH.
Example 2
Staphylococcus squirrel cultured by the above method（Staphylococcus sciuri）F-E8-1 is characterized as follows:
salt-tolerant bacteria staphylococcus squirrel（Staphylococcus sciuri）F-E8-1 has the characteristics that, for example, after the strain is cultured in an LB culture medium at 37 ℃ for 24 hours, a light yellow opaque single colony appears in the culture medium, the surface is moist and glossy, the edge is smooth, and the diameter is 3-4 mm; gram-positive G + cells are spherical and spore-free; the test is positive in Oxidase (OX) test, positive in catalase test, positive in gelatin liquefaction test, negative in amylase test, positive in glucose, maltose, sucrose, galactose and mannose test, and negative in mannitol, sorbitol, lactose, arabinose, arginine and xylose rhamnose test.
About Staphylococcus squirrel (Staphylococcus sciuri) The inventor verifies the treatment effect of F-E8-1 on high-salt fermentation wastewater through examples 3-8, and concretely comprises the following steps:
example 3
The staphylococcus squirrel is verified by adding the staphylococcus squirrel which is resistant to high salt and efficiently degraded into a high-salt fermentation wastewater treatment system (Staphylococcus sciuri) The treatment effect of F-E8-1 on the high-salt fermentation wastewater comprises the following specific steps:
glutamic acid wastewater containing 38% of salt and 3.16% of sugar content is taken and put into a fermentation tank, peptone is added to adjust the content of amino nitrogen in the wastewater to 0.8%, and the pH value is as follows: 8.2, the fermentation temperature is 35 ℃, and 12 x 10 is inoculated according to 1 percent8cfu g- 1The glucose content of the staphylococcus squirrel is reduced to 0.18 percent after the staphylococcus squirrel is cultured for 30 hours in a ventilating way.
Performing solid-liquid separation by plate-frame filter pressing, wherein the mesh number of the plate-frame filter cloth is 200 meshes, performing multi-effect evaporation on filtrate, and then drying by a fluidized bed at the drying temperature of 80 ℃ to obtain the crude salt with the ammonium sulfate content of 92.9 percent.
The results show that the use of Staphylococcus saimiri (A), (B), (C), (Staphylococcus sciuri) The F-E8-1 can achieve the purpose of rapidly degrading residual sugar in the fermentation wastewater for the treatment of the high-salinity fermentation wastewater.
Example 4
Staphylococcus saimiri（Staphylococcus sciuri）The steps of F-E8-1 for treating high-salt fermentation wastewater are as follows:
glutamic acid wastewater containing 21% of salt and 1.75% of sugar is taken and put into a fermentation tank, peptone is added to adjust the content of amino nitrogen in the wastewater to 1.0%, and the pH value is as follows: 5.0, fermentation temperature 33 ℃, inoculating 12 x 10 according to 1 percent8cfu g- 1The glucose content of the staphylococcus squirrel is reduced to 0.14 percent after the staphylococcus squirrel is cultured for 35 hours in a ventilating way.
Performing solid-liquid separation by plate-frame filter pressing, wherein the mesh number of the plate-frame filter cloth is 600 meshes, performing multi-effect evaporation on filtrate, and then drying by a fluidized bed at the drying temperature of 80 ℃ to obtain the crude salt with the ammonium sulfate content of 92.5 percent.
Example 5
Staphylococcus saimiri（Staphylococcus sciuri）The steps of F-E8-1 for treating high-salt fermentation wastewater are as follows:
putting glutamic acid wastewater containing 12% of salt and 1% of sugar into a fermentation tank, adding peptone to adjust the content of amino nitrogen in the wastewater to 0.55%, and adjusting the pH value: 5.8, the fermentation temperature is 30 ℃, and 17 multiplied by 10 percent of the fermentation liquor is inoculated according to 1 percent8cfu g- 1The glucose content of the staphylococcus squirrel is reduced to 0.06 percent after the staphylococcus squirrel is cultured for 37 hours in a ventilating way.
Solid-liquid separation is carried out by a disc centrifuge, the centrifugal force of the centrifuge is 6000g, the filtrate is subjected to multi-effect evaporation firstly, then fluidized bed drying is carried out, and the drying temperature is 100 ℃, so that the crude salt with the ammonium sulfate content of 92.3 percent is prepared.
Example 6
Staphylococcus saimiri（Staphylococcus sciuri）The steps of F-E8-1 for treating high-salt fermentation wastewater are as follows:
loading lysine wastewater containing 9.6% of salt and 0.75% of sugar into a fermentation tank, adding peptone to adjust the content of amino nitrogen in the wastewater to 0.65%, and adjusting the pH value: 8.5, the fermentation temperature is 37 ℃, and 20 multiplied by 10 percent of the fermentation liquor is inoculated according to 1 percent8cfu g- 1SquirrelAfter the staphylococcus is cultured for 28 hours in a ventilating way, the sugar content of the staphylococcus is reduced to 0.05 percent.
Solid-liquid separation is carried out by a disc centrifuge, the centrifugal force of the centrifuge is 21380g, the filtrate is subjected to multi-effect evaporation, and then fluidized bed drying is carried out, wherein the drying temperature is 60 ℃, and crude salt with the ammonium sulfate content of 91.8% is prepared.
Example 7
Staphylococcus saimiri（Staphylococcus sciuri）The steps of F-E8-1 for treating high-salt fermentation wastewater are as follows:
loading lysine wastewater containing 5% of salt and 2% of sugar into a fermentation tank, adding peptone to adjust the content of amino nitrogen in the wastewater to 0.80%, and adjusting the pH value: 4.5, the fermentation temperature is 30 ℃, and 20 multiplied by 10 are inoculated according to 1 percent8cfu g- 1The glucose content of the staphylococcus squirrel is reduced to 0.14 percent after the staphylococcus squirrel is cultured for 33 hours in a ventilating way.
Solid-liquid separation is carried out by a disc centrifuge, the centrifugal force of the centrifuge is 19000g, the filtrate is subjected to multi-effect evaporation firstly, then fluidized bed drying is carried out, and the drying temperature is 90 ℃, so that crude salt with the ammonium sulfate content of 91.4 percent is prepared.
Example 8
Staphylococcus saimiri（Staphylococcus sciuri）The steps of F-E8-1 for treating high-salt fermentation wastewater are as follows:
charging citric acid wastewater containing 5% of salt and 2% of sugar into a fermentation tank, adding peptone to adjust the content of amino nitrogen in the wastewater to 0.55%, and adjusting the pH value: 9.5, fermentation temperature is 27 ℃, 15 multiplied by 10 are inoculated according to 1 percent8cfu g- 1The sugar content of the staphylococcus squirrel is reduced to 0.16 percent after the staphylococcus squirrel is cultured for 42 hours in a ventilating way.
Solid-liquid separation is carried out by a disc centrifuge, the centrifugal force of the centrifuge is 2500g, the filtrate is subjected to multi-effect evaporation firstly, then fluidized bed drying is carried out, and the drying temperature is 90 ℃, so that crude salt with the ammonium sulfate content of 92% is prepared.
Example 9
The results of comparing the crude salt recoveries of examples 3-8 are shown in Table 1 below:
TABLE 1 Effect of examples on the treatment of wastewater from fermentation production
Treatment of The salt content of the high-salt wastewater is% Temperature of Duration of ventilation (h) The blood sugar content is reduced Content of crude salt%
Example 3 38 35 30 0.18 92.9
Example 4 21 33 35 0.14 92.5
Example 5 12 30 37 0.06 92.3
Example 6 9.6 37 28 0.05 91.8
Example 7 5 30 33 0.14 91.4
Example 8 5 27 42 0.16 92
As can be seen from the data in the above table, the Staphylococcus squirrel provided by the present invention（Staphylococcus sciuri）When the ventilation time of F-E8-1 is more than 30 hours at 35 ℃, the blood sugar reducing amount can reach 0.18 percent, and the yield of the crude salt can reach more than 91 percent. The purpose of the invention is realized, namely, the residual sugar in the organic acid and amino acid fermentation production wastewater is rapidly degraded in a high-salt environment. And the harmful influence on the wastewater is avoided during treatment.
The treatment effect of the traditional biological treatment method is greatly influenced by salt, and high-concentration inorganic salt can destroy cell membranes of microorganisms and enzymes in the microorganisms by increasing the osmotic pressure of the environment, so that the physiological activity of the microorganisms is destroyed, and the toxic action is generated on the biological treatment of the wastewater. The high-salt-resistant and high-efficiency-degradation staphylococcus squirrel is added into the high-salt fermentation wastewater treatment system, so that the biological treatment efficiency of the system is improved, the purpose of quickly degrading residual sugar in the fermentation wastewater is achieved, and after thalli are removed through solid-liquid separation, crude salt contained in the fermentation wastewater is concentrated and recovered. From the comparison above, it can be seen that the crude salt yield is high at higher temperatures and longer aeration times.

Claims (7)

1. Staphylococcus saimiri (A), (B), (C)Staphylococcus sciuri） The culture method of F-E8-1 is characterized in that the strain is preserved in China Center for Type Culture Collection (CCTCC) in 3-6 months of 2020, and the preservation number is M2020052;
the culture method comprises the following steps:
staphylococcus squirrel on LB plate (Staphylococcus sciuri） F-E8-1 single colony is inoculated in a seed culture medium, cultured for 12-18 h at 37-42 ℃, then inoculated into a fermentation culture medium according to the inoculum size of 4-6%, and cultured for 96-120 h at 37-42 ℃.
2. The Staphylococcus squirrel of claim 1 (S), (S) and S) A)Staphylococcus sciuri） F-E8-1, wherein the Staphylococcus squirrel (S.matsutake (L.)) (Staphylococcus sciuri） The seed culture medium of F-E8-1 comprises the following raw materials in percentage by weight: 0.35-0.65% of yeast powder, 0.8-1.2% of peptone and 0.35-0.65% of sodium chloride.
3. The Staphylococcus squirrel of claim 1 (S), (S) and S) A)Staphylococcus sciuri） F-E8-1, wherein the Staphylococcus squirrel (S.matsutake (L.)) (Staphylococcus sciuri） The fermentation medium of F-E8-1 comprises the following raw materials in percentage by weight: glutamic acid waste liquid containing 20-30 wt% of ammonium sulfate and the balance of sterile water, and adjusting the pH value to 7.0-7.5 by adopting 1M NaOH.
4. The Staphylococcus squirrel of claim 1 (S), (S) and S) A)Staphylococcus sciuri） F-E8-1, wherein the Staphylococcus squirrel (S.matsutake (L.)) (Staphylococcus sciuri） After the F-E8-1 is cultured in a fermentation medium, the residual sugar in the wastewater is reduced to 0.2-0.5%.
5. The Staphylococcus squirrel of claim 1 (S), (S) and S) A)Staphylococcus sciuri） The culture method of F-E8-1, characterized in that the staphylococcus squirrel (A)Staphylococcus sciuri） Culturing the F-E8-1 strain in an LB culture medium at 37 ℃ for 24h, wherein the culture medium presents a light yellow opaque single colony, the surface is moist and glossy, the edge is smooth, and the diameter is 3-4 mm; gram-positive G + cells are spherical and spore-free; the test is positive in oxidase test, positive in catalase test, positive in gelatin liquefaction test and negative in amylase test, the tests of glucose, maltose, sucrose, galactose and mannose are all positive, and the tests of mannitol, sorbitol, lactose, arabinose, arginine, xylose and rhamnose are all negative.
6. The use of Staphylococcus squirrel (Staphylococcus sciuri) F-E8-1 cultured by the culture method of claim 1 in the high-salt environment to rapidly degrade residual sugar in glutamic acid wastewater, lysine wastewater or citric acid wastewater.
7. The use according to claim 6, wherein the wastewater has a salt content of between 5 and 40%.
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