CN111729068B - Application of external pet spray in preparation of medicine for inhibiting feline streptococcus - Google Patents
Application of external pet spray in preparation of medicine for inhibiting feline streptococcus Download PDFInfo
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- CN111729068B CN111729068B CN202010640434.3A CN202010640434A CN111729068B CN 111729068 B CN111729068 B CN 111729068B CN 202010640434 A CN202010640434 A CN 202010640434A CN 111729068 B CN111729068 B CN 111729068B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
- A61K9/0017—Non-human animal skin, e.g. pour-on, spot-on
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/12—Aerosols; Foams
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides an external pet spray and a preparation method thereof, belonging to the technical field of pet breeding and comprising the following components in parts by weight: 60-70 parts of antibacterial peptide extract, 5-10 parts of callicarpa nudiflora extract, 2-3 parts of lavender essential oil, 7-10 parts of emulsifier, 1-2 parts of pH value regulator and 5-26.5 parts of water; the raw materials of the antibacterial peptide extracting solution comprise pig small intestine and cow small intestine. The external pet spray provided by the invention has obvious inhibition effects on canine-derived large intestine antibiosis, canine-derived staphylococcus aureus and feline-derived streptococcus, can reduce wound stimulation and promote wound healing, and is convenient to use.
Description
Technical Field
The invention belongs to the technical field of pet breeding, and particularly relates to an external pet spray and a preparation method thereof.
Background
Pets have been good partners for humans, like cats, dogs, rabbits, etc. Pets are also struggled for food, pets and partners for a long time, and the pets are often injured in different degrees, blood is dribbling, and if the pets are not treated in time, the pets can be seriously infected and even the life safety is influenced. Traditional external medicine often adopts chemical medicine to disinfect to cooperate the antibiotic to treat it, just use chemical medicine to strong to skin irritation, restricted the healing of wound, the antibiotic produces the drug resistance easily, and can produce the side effect to the organism.
Disclosure of Invention
In view of the above, the invention aims to provide an external pet spray and a preparation method thereof, and the external pet spray provided by the invention has a remarkable inhibiting effect on canine-derived large intestine antibacterial, canine-derived staphylococcus aureus and feline-derived streptococcus, can reduce wound stimulation and promote wound healing, and is convenient to use.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides an external pet spray which comprises the following components in parts by weight: 60-70 parts of antibacterial peptide extract, 5-10 parts of callicarpa nudiflora extract, 2-3 parts of lavender essential oil, 7-10 parts of emulsifier, 1-2 parts of pH value regulator and 5-26.5 parts of water;
the raw materials of the antibacterial peptide extracting solution comprise pig small intestines and cow small intestines.
Preferably, the composition comprises the following components in parts by weight: 65 parts of antibacterial peptide extract, 7 parts of callicarpa nudiflora extract, 2.5 parts of lavender essential oil, 8 parts of emulsifier, 1-2 parts of pH value regulator and 25 parts of water.
Preferably, the mass ratio of the pig small intestine to the cow small intestine is 3-5: 2-4.
Preferably, the preparation method of the antibacterial peptide extracting solution comprises the following steps:
1) mixing small intestine of pig and small intestine of cattle with acetic acid solution, mashing, and homogenizing to obtain homogenate;
2) freezing and thawing the homogenate obtained in the step 1) to obtain a frozen and thawed matter, and centrifuging the frozen and thawed matter to obtain a supernatant;
3) filtering the supernatant obtained in the step 2) with a 0.22-micron filter membrane to obtain a filtrate, performing ultrafiltration on the filtrate with a 10KD filter membrane to obtain an ultrafiltration substance, and filtering the ultrafiltration substance with a 0.1-micron sterilizing filter to sterilize, wherein the obtained filtrate is an antibacterial peptide extract.
Preferably, the number of freeze thawing in the step 2) is 6.
Preferably, the centrifugation conditions of step 2) include: centrifuge at 7000rpm at 5 ℃ for 15 min.
Preferably, the preparation method of the callicarpa nudiflora extract comprises the following steps:
A. mixing leaves of Callicarpa nudiflora with water, mashing, and homogenizing to obtain homogenate;
B. freezing and thawing the homogenate obtained in the step A to obtain a frozen and thawed matter, and centrifuging the frozen and thawed matter to obtain a supernatant;
C. and B, sequentially filtering the supernatant obtained in the step B through a 0.22-micrometer filter membrane, a 5KD filter membrane and a 0.1-micrometer sterilizing filter for sterilization to obtain filtrate which is callicarpa nudiflora extract.
Preferably, the emulsifier comprises Montanide TM ISA201VG mineral oil and Tween-80, the Montanide TM The mass ratio of ISA201VG mineral oil to Tween-80 is 9.5: 0.5.
Preferably, the pH value regulator comprises sodium bicarbonate solution, and the mass concentration of the sodium bicarbonate solution is 30%.
The invention also provides a preparation method of the pet external spray in the technical scheme, which comprises the following steps:
a. mixing the water, the antibacterial peptide extracting solution, the callicarpa nudiflora extracting solution, the lavender essential oil and the pH value regulator to obtain a mixed solution;
b. and c, mixing the mixed liquid obtained in the step a with an emulsifier, and stirring at 4100-4300 rpm for 28-38 min to obtain the pet external spray.
The invention provides a pet external spray which comprises the following components in parts by weight: 60-70 parts of antibacterial peptide extract, 5-10 parts of callicarpa nudiflora extract, 2-3 parts of lavender essential oil, 7-10 parts of emulsifier, 1-2 parts of pH value regulator and 5-26.5 parts of water; the raw materials of the antibacterial peptide extracting solution comprise pig small intestine and cow small intestine.
Detailed Description
The invention provides an external pet spray which comprises the following components in parts by weight: 60-70 parts of antibacterial peptide extract, 5-10 parts of callicarpa nudiflora extract, 2-3 parts of lavender essential oil, 7-10 parts of emulsifier, 1-2 parts of pH value regulator and 5-26.5 parts of water; the raw materials of the antibacterial peptide extracting solution comprise pig small intestines and cow small intestines.
The external pet spray provided by the invention comprises 60-70 parts by weight of antibacterial peptide extract, preferably 65 parts by weight. In the invention, the antibacterial peptide extracting solution has the function that the antibacterial peptide can directly kill pathogenic microorganisms in vitro and has broad-spectrum antibacterial activity. Not only can resist G + 、G - Bacteria, fungi, protozoa and other pathogenic microorganisms. It can also be used for resisting tumor and cancer cell, and even can be used for directly killing enveloped influenza virus, herpes virus, AIDS virus, etc. In the invention, the raw materials of the antibacterial peptide extracting solution comprise pig small intestine and cow small intestine, and the pig small intestine and the cow small intestine contain abundant antibacterial peptide substances. In the invention, the mass ratio of the pig small intestine to the cow small intestine is preferably 3-5: 2-4.
In the present invention, the method for preparing the antibacterial peptide extract preferably comprises the steps of:
1) mixing small intestine of pig and small intestine of cattle with acetic acid solution, mashing, and homogenizing to obtain homogenate;
2) freezing and thawing the homogenate obtained in the step 1) to obtain a frozen and thawed substance, and centrifuging the frozen and thawed substance to obtain a supernatant;
3) filtering the supernatant obtained in the step 2) with a 0.22-micrometer filter membrane to obtain a filtrate, performing ultrafiltration on the filtrate with a 10KD filter membrane to obtain an ultrafiltration substance, and filtering the ultrafiltration substance with a 0.1-micrometer sterilizing filter to sterilize, wherein the obtained filtrate is an antibacterial peptide extract.
In the present invention, the content of the acetic acid solution is preferably 1% by mass. In the present invention, the mass ratio of the total mass of the porcine small intestine and the bovine small intestine to the acetic acid solution is preferably 1: 1. In the invention, the content of the polypeptide in the antibacterial peptide extracting solution is 650-759 mu L/mL, preferably 682 mu L/mL.
The method of the present invention is not particularly limited to the above-mentioned mashing and homogenizing step, and may be carried out by a conventional method.
In the present invention, the number of freeze-thawing is preferably 6. In the present invention, the conditions of the centrifugation preferably include: centrifuge at 7000rpm at 5 ℃ for 15 min.
The external pet spray provided by the invention comprises 5-10 parts by weight of callicarpa nudiflora extract, preferably 7 parts by weight. In the invention, the callicarpa nudiflora extract has the functions of diminishing inflammation and sterilizing. In the present invention, the preparation method of the callicarpa nudiflora extract preferably comprises the following steps:
A. mixing leaves of Callicarpa nudiflora with water, mashing, and homogenizing to obtain homogenate;
B. freezing and thawing the homogenate obtained in the step A to obtain a frozen and thawed matter, and centrifuging the frozen and thawed matter to obtain a supernatant;
C. and B, sequentially filtering the supernatant obtained in the step B through a 0.22-micron filter membrane, a 5KD filter membrane and a 0.1-micron sterilizing filter for sterilization, and obtaining filtrate which is the callicarpa nudiflora extracting solution.
The conditions for the mashing and the homogenizing are not particularly limited in the present invention, and the conventional method may be adopted.
In the present invention, the number of times of freezing and thawing is preferably 4 times. In the present invention, the conditions of the centrifugation preferably include: centrifuge at 7000rpm for 15min at 5 ℃.
The external pet spray provided by the invention comprises 2-3 parts by weight of lavender essential oil, preferably 2.5 parts by weight. In the invention, the lavender essential oil has the effects of removing scars on the skin and increasing the fragrance of the spray. The source of the lavender essential oil is not particularly limited, and a commercially available product is adopted.
The external pet spray provided by the invention comprises 7-10 parts by weight of emulsifier, preferably 8 parts by weight. In the present invention, the emulsifier preferably comprises Montanide TM ISA201VG mineral oil and Tween-80, the Montanide TM The mass ratio of ISA201VG mineral oil to Tween-80 is preferably 9.5: 0.5.
The external pet spray provided by the invention comprises 1-2 parts by weight of a pH value regulator. In the present invention, the pH adjuster preferably includes a sodium bicarbonate solution, and the sodium bicarbonate solution preferably has a mass concentration of 30%.
In the invention, the preparation method of the external pet spray comprises the following steps:
a. mixing the water, the antibacterial peptide extracting solution, the callicarpa nudiflora extracting solution, the lavender essential oil and the pH value regulator to obtain a mixed solution;
b. and c, mixing the mixed liquid obtained in the step a with an emulsifier, and stirring at 4100-4300 rpm for 28-38 min to obtain the pet external spray.
In the present invention, the method for using the pet external spray preferably comprises spraying the spray on the wound until the surface of the wound is uniformly covered.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The external pet spray comprises, by mass, 60% of an antibacterial peptide extracting solution, 5% of a callicarpa nudiflora extracting solution, 2% of lavender essential oil, 7% of an emulsifier, 1% of a pH regulator and the balance purified water. WhereinThe emulsifier is Montanide TM ISA201VG mineral oil and TWEEN-80 in a ratio of Montanide TM ISA201 VG: TWEEN-80 ═ 9.5: 0.5; the pH regulator is sodium bicarbonate solution with the mass percentage of 30 percent.
The preparation method of the callicarpa nudiflora extract comprises the following steps:
(1) cleaning fresh callicarpa nudiflora leaves with purified water, adding the purified water according to the mass percentage of 1:1, and mashing with a mashing machine to obtain a mashed material.
(2) And repeatedly freezing and thawing the obtained triturate for 4 times, taking a frozen and thawed liquid, placing at 5 ℃ for 7000 r/min, and centrifuging for 15min to obtain a centrifugal supernatant.
(3) Taking the centrifugal supernatant, performing tangential flow microfiltration by adopting a 0.22 mu m filter membrane, and collecting microfiltration permeate.
(4) Performing tangential flow ultrafiltration on the microfiltration permeate by adopting a 5KD filter membrane, and collecting the ultrafiltration permeate.
(5) Sterilizing the ultrafiltration permeate by using a 0.1 mu m sterilizing filter, and collecting filtrate to obtain the callicarpa nudiflora extract.
The preparation method of the antibacterial peptide extracting solution comprises the following steps:
(1) adding 1% by mass of acetic acid solution into cleaned small intestine (pig small intestine and cattle small intestine at a mass ratio of 3:2) at a mass ratio of 1:1, mashing with a mashing machine, and homogenizing with a colloid mill to obtain homogenate.
(2) And repeatedly freezing and thawing the homogenate for 6 times, taking the frozen and thawed liquid, placing at 5 ℃ for 7000 r/min, and centrifuging for 15 min.
(3) Removing the centrifugal supernatant, performing tangential flow microfiltration on the filtrate by using a 0.22 mu m filter membrane, and collecting microfiltration permeate.
(4) Performing tangential flow ultrafiltration on the microfiltration permeate by adopting a 10KD filter membrane, and collecting the ultrafiltration permeate.
(5) Sterilizing the ultrafiltration permeate by adopting a 0.1 mu m sterilizing filter, and collecting filtrate to obtain the antibacterial peptide extract. The content of the detected polypeptide is 650 mu l/ml.
The preparation method of the pet external spray comprises the following steps:
the method comprises the following steps: adding antibacterial peptide extract, Callicarpa nudiflora extract and Lavender essential oil into purified water at a certain proportion, stirring with a stirrer, adding pH regulator to adjust pH to 5.0 to obtain solution A.
Step two: adding the emulsifier into the solution A, and stirring for 35 minutes by using a high-speed stirrer 4200 r/min to obtain the pet external spray.
Example 2
The pet external spray comprises 65 mass percent of antibacterial peptide extract, 7 mass percent of callicarpa nudiflora extract, 2.5 mass percent of lavender essential oil, 8 mass percent of emulsifier, 1 mass percent of pH regulator and the balance of purified water. Wherein the emulsifier is Montanide TM ISA201VG mineral oil and TWEEN-80 in Montanide ratio TM ISA201 VG: TWEEN-80 ═ 9.5: 0.5; the pH regulator is sodium bicarbonate solution with the mass percentage of 30 percent.
The preparation method of the callicarpa nudiflora extract is the same as that of example 1.
The preparation method of the antibacterial peptide extract is the same as that in example 1, except that the mass ratio of the porcine small intestine to the bovine small intestine is 4: 3. The content of the detected polypeptide is 682 mu l/ml.
The antibacterial spray for pet external use is prepared in the same manner as in example 1.
Example 3
The external pet spray comprises, by mass, 70% of an antibacterial peptide extracting solution, 10% of a callicarpa nudiflora extracting solution, 3% of lavender essential oil, 10% of an emulsifier, 2% of a pH regulator and the balance purified water. Wherein the emulsifier is Montanide TM ISA201VG mineral oil and TWEEN-80 in a ratio of Montanide TM ISA201 VG: TWEEN-80 ═ 9.5: 0.5; the pH regulator is sodium bicarbonate solution with the mass percentage of 30 percent.
The preparation method of the callicarpa nudiflora extract is the same as that of example 1.
The preparation method of the antibacterial peptide extract is the same as that in example 1, except that the mass ratio of the porcine small intestine to the bovine small intestine is 5: 4. The content of the detected polypeptide is 759 mu l/ml.
The preparation method of the pet external spray is the same as that of example 1.
Example 4
Bacteriostatic test
The 3 batches of pet external sprays prepared in examples 1, 2 and 3 (numbered 001, 002 and 003 respectively) were applied to agar plates full of canine-derived antibacterial large intestine, canine-derived staphylococcus aureus and feline-derived streptococcus respectively with self-made drug sensitive paper sheets and other drug paper sheets commonly used in 12 to perform drug sensitivity tests. The paper pieces are uniformly stuck on an agar plate by using tweezers, and after the paper pieces are placed in a constant temperature box at 37 ℃ for 18 hours, the result is observed, the diameter of a bacteriostatic ring is measured, and the result is shown in table 1 by referring to CLSIM100-S20 according to the judgment standard.
TABLE 1 antibacterial test results (mm) of antibacterial sprays against caninum Escherichia coli, caninum Staphylococcus aureus and fel Streptococci
Note: s is sensitive, M is intermediate, R is tolerant
As can be seen from Table 1, all the 3 tested strains of bacteria are multi-drug resistant bacteria, wherein the canine escherichia coli is only sensitive to amikacin and neomycin; staphylococcus aureus of canine origin is sensitive only to amoxicillin; feline streptococci are only sensitive to rifampicin and spectinomycin. Starting from the diameter of the inhibition zone, the 003 batches of external pet spray prepared by the formula of example 3 have the largest diameter of the inhibition zone in all the drug sensitive paper sheets, and have the following diameters of the inhibition zones for canine-derived large intestine, canine-derived staphylococcus aureus and feline-derived streptococcus respectively: 23mm, 22mm and 26mm, and has good antibacterial effect. The test result shows that the external pet spray prepared by the invention can replace antibiotics to achieve better antibacterial effect.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. The application of the pet external spray in preparing the medicine for inhibiting the feline streptococcus is characterized in that the pet external spray is composed of the following components in parts by weight: 60-70 parts of antibacterial peptide extract, 5-10 parts of callicarpa nudiflora extract, 2-3 parts of lavender essential oil, 7-10 parts of emulsifier, 1-2 parts of pH value regulator and 5-26.5 parts of water;
the raw materials of the antibacterial peptide extracting solution are pig small intestine and cattle small intestine;
the preparation method of the antibacterial peptide extracting solution comprises the following steps:
1) mixing small intestine of pig and small intestine of cattle with acetic acid solution, mashing, and homogenizing to obtain homogenate;
2) freezing and thawing the homogenate obtained in the step 1) to obtain a frozen and thawed substance, and centrifuging the frozen and thawed substance to obtain a supernatant;
3) filtering the supernatant obtained in the step 2) with a 0.22-micrometer filter membrane to obtain a filtrate, performing ultrafiltration on the filtrate with a 10KD filter membrane to obtain an ultrafiltration substance, and filtering the ultrafiltration substance with a 0.1-micrometer sterilizing filter to sterilize, wherein the obtained filtrate is an antibacterial peptide extract.
2. The application of claim 1, wherein the external pet spray comprises the following components in parts by weight: 65 parts of antibacterial peptide extract, 7 parts of callicarpa nudiflora extract, 2.5 parts of lavender essential oil, 8 parts of emulsifier, 1-2 parts of pH value regulator and 25 parts of water.
3. The use of claim 1, wherein the ratio of the mass of the porcine intestine to the mass of the bovine intestine is (3-5) to (2-4).
4. The use of claim 1, wherein the number of freeze-thawing in step 2) of the method for preparing the antibacterial peptide extract is 6.
5. The use according to claim 1, wherein the antibacterial peptide extract is prepared by a method comprising the following centrifugation conditions in step 2): centrifuge at 7000rpm at 5 ℃ for 15 min.
6. The use according to claim 1 or 2, wherein the preparation method of the callicarpa nudiflora extract comprises the following steps:
A. mixing leaves of Callicarpa nudiflora with water, mashing, and homogenizing to obtain homogenate;
B. freezing and thawing the homogenate obtained in the step A to obtain a frozen and thawed matter, and centrifuging the frozen and thawed matter to obtain a supernatant;
C. and B, sequentially filtering the supernatant obtained in the step B through a 0.22-micron filter membrane, a 5KD filter membrane and a 0.1-micron sterilizing filter for sterilization, and obtaining filtrate which is the callicarpa nudiflora extracting solution.
7. The use according to claim 1 or 2, wherein the emulsifier comprises montanide tmisa201VG mineral oil and tween-80, the mass ratio of montanide tmisa201VG mineral oil to tween-80 being 9.5: 0.5.
8. The use according to claim 1 or 2, wherein the pH regulator comprises a sodium bicarbonate solution having a concentration of 30% by mass.
9. The use as claimed in any one of claims 1 to 5, wherein the preparation method of the external pet spray comprises the following steps:
a. mixing the water, the antibacterial peptide extracting solution, the callicarpa nudiflora extracting solution, the lavender essential oil and the pH value regulator to obtain a mixed solution;
b. and c, mixing the mixed liquid obtained in the step a with an emulsifier, and stirring at 4100-4300 rpm for 28-38 min to obtain the pet external spray.
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| CN106916218A (en) * | 2017-05-09 | 2017-07-04 | 派生特（福州）生物科技有限公司 | A kind of preparation method of Antibacterial Peptide Extracted from Pig Small Intestine |
| CN106977595A (en) * | 2017-05-09 | 2017-07-25 | 派生特（福州）生物科技有限公司 | The extracting method of one boar derived antimicrobial peptide |
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| CN107510622A (en) * | 2017-08-30 | 2017-12-26 | 安徽省中日农业环保科技有限公司 | A kind of preparation method of the anti-acne repair latex containing Lavender |
| CN109464320A (en) * | 2018-11-09 | 2019-03-15 | 厦门舒菲娅化妆品有限公司 | A kind of baby baths liquid and preparation method thereof |
| CN110236070A (en) * | 2019-07-31 | 2019-09-17 | 华中农业大学 | A kind of pure essential oil antimicrobial emulsion, preparation method and applications |
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