CN111718837A

CN111718837A - Kit and method for obtaining sperms on extracted testicular tissue seminiferous tubules

Info

Publication number: CN111718837A
Application number: CN202010578630.2A
Authority: CN
Inventors: 伍玲园; 张亮; 杨慧军; 吕雪梅; 董云玲; 陶国振; 范媛媛; 雷丽君; 刘皎婧; 盛连兵; 董芳地; 顾光燕; 于娜
Original assignee: Shandong Maternal And Child Health Hospital
Current assignee: Shandong Maternal And Child Health Hospital
Priority date: 2020-06-23
Filing date: 2020-06-23
Publication date: 2020-09-29

Abstract

The invention belongs to the technical field of reproductive medicine tools, and particularly relates to a kit for improving semen collection efficiency. The kit of the invention mainly comprises: fixing device and dialling device. The fixing device includes: the fixing rod, the first connecting rod, the second connecting rod and the fixing frame. The first connecting rod is detachably connected below the fixing rod, the second connecting rod is connected below the first connecting rod, and the second connecting rod is connected with the fixing frame. The dialing device comprises: tissue picking rod, third connecting rod, tissue picking device. A third connecting rod is detachably connected below the tissue poking rod, and a tissue poking device is connected below the third connecting rod. The kit has the advantages that by using the kit, testicular tissues can be well fixed, damage to sperms in the traditional sperm extraction process is avoided, and the success probability of sperm extraction is obviously improved.

Description

Kit and method for obtaining sperms on extracted testicular tissue seminiferous tubules

Technical Field

The invention belongs to the technical field of reproductive medical tools, and particularly relates to a kit for separating sperms from a seminiferous tubule in a picked testicular tissue, and a using method of the kit.

Background

Azoospermia clinically accounts for 10% to 15% of male infertility patients, and of these, about 50.0% is caused by non-obstructive factors. Non-obstructive azoospermia (NOA) is a common disease and a difficult disease in clinical male reproduction, the etiology mechanism of the NOA is complex and changeable, and the NOA is caused by testicular spermatogenic dysfunction caused by various factors. In this fraction of NOA patients, there were some spermatogenic foci in the seminiferous tubules in the testicular tissue, where sperm could be obtained. Clinically, a procreation andriatrist may use open testicular biopsy (TESE) or testicular microscopical (M-TESE) to obtain testicular tissue where spermatogenic foci may be present. Typically, after the andriatrist cuts the testicular tissue, the andriatrist gives the andriatrist the embryologist who needs to separate the valuable sperm from the seminiferous tubules where spermatogenic foci may be present. The current laboratory practice is primarily with the aid of syringes, the main principle being repeated aspiration to obtain sperm. The disadvantage is that the testis surface has thick dense connective tissue membrane, the mediastinum inside is converged into testis net, and the mediastinum extends into the essence of testis in radial shape, dividing the testis into several small leaves, each small leaf contains 1-3 curved seminiferous tubules, these seminiferous foci exist in the seminiferous tubules. The sperm cannot be completely separated from the seminiferous tubules by the conventional syringe repeated aspiration method.

CN105695315A discloses a device for efficiently scraping and crushing a curved and fine sperm tube to obtain sperms, which is characterized in that; the device includes: the scraping needle comprises a scraping needle (1), a fixed needle sleeve (2), a handheld handle (3) and a scraping needle sleeve (4); one end of a long rod-shaped handheld handle (3) is provided with a fixed needle sleeve (2), the fixed needle sleeve (2) is sleeved and fixed on the handheld handle (3), a plurality of comb-shaped scraping needles (1) are fixedly arranged on the top surface of the fixed needle sleeve (2), and the scraping needles (1) are sleeved with a scraping needle sleeve (4) and clamped on the fixed needle sleeve (2).

The above patent documents are provided with multi-needle scraping needles, which have the disadvantages that the tips of the scraping needles are very easy to cause damage to sperms, and the sperms in the testis are not mature enough, which can be embodied in that the nuclei are not completely condensed, the mobility is not good, the acrosome reaction is not sufficient, and the sperms at the time are very easy to be damaged by external stimulation and are positioned in the acrosome area of the head, thereby causing bad influence on fertilization of the embryo and the development and pregnancy of the subsequent embryo.

Therefore, there is a need to improve upon the above-mentioned technical problems and to develop a device that can effectively reduce the damage to sperm during the semen collection process and can sufficiently collect a sufficient number of sperm.

Disclosure of Invention

In order to solve the technical problems, the invention provides a kit for taking sperms from the taken testicular tissues, which can quickly, smoothly and effectively obtain enough sperms without damaging the sperms and can also reduce the damage to the sperms in the sperm taking process; and the suit tool is stable and simple and convenient to install.

The invention provides a kit for extracting sperms from extracted testicular tissues, which comprises: the device comprises a fixing device and a dialing device;

the fixing device includes: the fixing rod, the first connecting rod, the second connecting rod and the fixing frame;

the fixed frame is any one of an equilateral triangle frame, a conical frame, a rectangular frame, a diamond frame and a circular frame; the fixing frame is made of medical transparent antibacterial organic glass material;

a first connecting rod is detachably connected below the fixed rod; a second connecting rod is connected below the first connecting rod and is connected with the fixed frame;

the second connecting rod comprises three extended claw-shaped rods; the angles between adjacent claw-shaped rods are the same;

the dialing device comprises: a tissue plucking rod, a third connecting rod and a tissue plucking device;

a third connecting rod is detachably connected below the tissue poking rod, and a tissue poking device is connected below the third connecting rod;

the tissue extractor is made of medical transparent antibacterial organic glass material;

the appearance structure of the tissue extractor is in a hook shape, a U shape, an L shape, a C shape or a ring shape, and the tail end of the tissue extractor is in a non-tip structure;

the tail end of the tissue extractor is of a smooth arc structure; or the tissue extractor is provided with a smooth arc-shaped structure;

preferably, the tissue extractor is L-shaped, and the L-shaped bending part of the tissue extractor is of a smooth arc structure.

A first handle is arranged above the fixed rod; a second handle is arranged above the poking rod;

or the side surface of the fixed rod is provided with a first handle, and the side surface of the poking rod is provided with a second handle;

preferably, the tissue extractor and the third connecting rod are welded;

preferably, the tissue extractor and the third connecting rod are in an integral structure;

preferably, the tissue extracting rod is in clamping connection with the third connecting rod or in threaded connection.

The outer surface of the fixed rod and/or the tissue poking rod is provided with an anti-slip rubber layer.

In the second connecting rod, three claw-shaped rods are respectively connected with three end points of the triangular fixing frame; or the three claw-shaped rods are respectively connected with the middle points of the three sides of the triangular fixing frame;

preferably, the second connecting rod is welded to the first connecting rod.

The second connecting rod and the first connecting rod are in an integral structure;

the first connecting rod is connected with the fixed rod through threads;

the first connecting rod is clamped and connected with the fixed rod;

preferably, a groove is formed in the lower portion of the fixing rod, internal threads are formed in the groove, external threads are formed at the upper end of the first connecting rod, and the first connecting rod is connected with the fixing rod through threads;

preferably, a groove is formed in the lower portion of the fixed rod, internal threads are formed in the groove, external threads are formed at the upper end of the first connecting rod, and the first connecting rod is connected with the fixed rod through threads; and a bolt is arranged outside the first connecting rod, and the first connecting rod and the fixed rod are further fixed through the bolt.

The use method of the kit for extracting sperms from the extracted testicular tissues comprises the following steps:

in the actual use process, a clinician puts a testicular tissue seminiferous tubule taken out by biopsy into a culture dish filled with buffer solution to clean and remove redundant blood cell impurities, then transfers the testicular tissue seminiferous tubule into the culture dish containing sperm washing solution, in an IVF workstation, the temperature of a hot table is set to be 36.8-37.2 ℃, preferably 37 ℃, under a stereoscope, a fixing device in a suit tool is held by the left hand, the seminiferous tubule is fixed by a fixing frame below a fixing rod of the fixing device, a right hand-held drawing device draws the seminiferous tubule under the visible state of a microscope by a tissue drawing device of the right hand, and when the seminiferous tubule is drawn sufficiently, the sperms are made to fully swim into the washing solution, the swimming condition of the sperms is observed under the inverted microscope, and then the seminiferous tubules are recovered and centrifuged to be used for subsequent injection of the.

The invention has the beneficial effects that:

(1) the fixing device is used for well fixing the picked testicle tissue, the picking device is used for efficiently separating sperms from the seminiferous tubules of the testicle tissue, and the fixing device and the picking device are used in a matched mode, so that the defects that the testicle tissue is easy to slide, cannot be fixed and is inconvenient to pick sperms when the sperms are picked are overcome;

(2) the dialing device is in a smooth hook shape, or in a U shape, or in an L shape, or in a circular shape; the structure is smooth, and the damage of the tip of the injector to sperms is avoided; the injury to sperms caused by scraping of the traditional sperm taking tool injector is avoided, and the sperms can be obtained quickly and efficiently by combining a fixing device, so that the success rate of the clinical sperm taking operation is greatly improved, a good foundation is provided for subsequent pregnancy of the NOA patient, and the secondary wound of repeated sperm taking on the NOA patient can be avoided.

(3) According to the fixing device and the pulling device, the fixing rod of the fixing device and the pulling rod of the pulling device can be used as recyclable medical instruments, only the fixing frame and the tissue puller need to be replaced during each operation, so that the cost is saved, and the economic burden of a patient is reduced;

(4) according to the triangular fixing frame of the fixing device, the stability and the tissue fixing effect of the triangular fixing frame are best, the testicle tissue is favorably fixed, and the triangular fixing frame is transparent antibacterial organic glass and can be favorably used for clearly visible operation under a microscope;

(5) the invention has simple structure, easy popularization and application and strong practicability in clinical application.

Drawings

FIG. 1 is a schematic view of the overall structure of an apparatus according to embodiment 1 of the present invention;

FIG. 2 is a schematic view of the structure of a fixing device according to embodiment 1;

FIG. 3 is an enlarged view of the fixing apparatus in embodiment 1;

FIG. 4 is a schematic view of the structure of a fixing device according to embodiment 2;

FIG. 5 is a schematic view of the structure of the drawer in embodiment 3;

FIG. 6 is a schematic view showing the structure of a drawer in embodiment 4;

FIG. 7 is a schematic view showing the construction of a drawer in embodiment 5;

FIG. 8 is a schematic view showing the structure of a fixing apparatus according to embodiment 6;

FIG. 9 is a schematic view of the structure of a fixing device according to embodiment 7;

FIG. 10 is a schematic view showing the structure of a fixing apparatus according to embodiment 8;

FIG. 11 is an enlarged schematic view of a fixing device according to embodiment 9;

in the figure, 1-fixation means, 101-fixation rod, 102-first connection rod, 103-second connection rod, 104-fixation frame, 105-internal thread, 106-external thread, 107-bolt, 108-handle, 109-rubber layer, 110-recess;

2-plucking device, 201-tissue plucking rod, 202-third connecting rod, 203-tissue plucker.

Detailed Description

In order that those skilled in the art will better understand the present invention, the present inventors shall further illustrate the present invention by the following specific examples, but shall not therefore limit the scope of the present invention.

Example 1

A kit for extracting sperms from extracted testicular tissues, mainly comprising: the device comprises a fixing device and a dialing device;

the fixing device 1 includes: a fixed rod 101, a first connecting rod 102, a second connecting rod 103 and a fixed frame 104;

a first connecting rod 102 is detachably connected below the fixing rod 101; a second connecting rod 103 is welded below the first connecting rod 102, and the second connecting rod 103 is welded with the fixing frame 104; the mounting bracket 104 is of a triangular design; the fixed frame 104 is an equilateral triangle frame; the fixing frame 104 is made of medical antibacterial transparent organic glass material; the second connecting rod 103 comprises three extended claw-shaped rods; the angles between adjacent claw-shaped rods are the same; the lower ends of the claw-shaped rods are respectively connected with three end points of the fixing frame 104;

according to the fixing device 1 and the pulling device 2, the fixing rod 101 of the fixing device 1 and the tissue pulling rod 201 of the pulling device 2 can be used as a recyclable medical instrument for recycling, and only connecting rods, the fixing frame 4 and the tissue puller 203 need to be replaced during each operation, so that the cost is saved, and the economic burden of a patient is reduced;

with such an arrangement, in the actual use process, only the first connecting rod 102, the second connecting rod 103 and the fixing frame 104 below the fixing rod 101 can be replaced; the fixing rod 101 can be repeatedly recycled so as to save resources;

a cylindrical groove is formed in the lower portion of the fixing rod 101, internal threads are formed in the groove, external threads are formed on the outer surface of the upper portion of the first connecting rod 102 and matched with the internal threads in the groove, and the fixing rod 101 and the first connecting rod 102 are connected through threads to fix the positions of the fixing rod 101 and the first connecting rod 102;

the setting device 2 includes: a tissue plucking rod 201, a third connecting rod 202, a tissue plucker 203;

the lower part of the tissue poking rod 201 is detachable and is connected with a third connecting rod 202, and a tissue poking device 203 is welded below the third connecting rod 202; the tissue extractor 203 is in the shape of a hook, and the tissue extractor 201 and the third connecting rod 202 are also connected by threads, and the specific connection is the same as that of the fixing rod 101 and the first connecting rod 102.

The tissue extractor 203 is made of medical antibacterial transparent organic glass. The tissue extractor 203 is L-shaped, the L-shaped bending part of the tissue extractor 203 is a smooth arc structure, the tail end of the tissue extractor is a smooth and smooth arc structure, and the tip of the tail end is an arc surface.

In actual use, the testicle tissue is held down by the fixation device 1.

Then the pulling device 2 (mainly the hook-shaped tissue pulling device 203) pulls the seminiferous tubules of the testicular tissue, so that the seminiferous tubules in the testicular tissue can be fully scraped, and the sperms in the seminiferous tissue flow into a dish filled with a culture solution, so that the sperms are obtained.

In the actual use process, a clinician puts a testicular tissue seminiferous tubule taken out by biopsy into a culture dish filled with buffer solution to clean and remove redundant blood cell impurities, then transfers the testicular tissue seminiferous tubule into the culture dish containing sperm washing solution, in an IVF workstation, the temperature of a hot table is set to be about 37 ℃, under a stereoscope, the left hand holds the fixing device 1 in the sleeving tool, the fixing frame 104 below the fixing rod 101 is used for fixing the seminiferous tubule, the right hand holds the poking device 2 to poke in a microscope visual state through the tissue poking device 203, under the condition that the seminiferous tubule is fully poked, the sperms are fully enabled to swim into the washing solution, the wandering condition of the sperms is observed under the microscope, and then the centrifuge is recovered to wait for subsequent injection of the single sperms in the ootheca, thereby completing the sperming process.

The biggest difference between the method and the traditional method for extracting the essence by adopting the injector is as follows:

(1) the method for taking sperm by the injector is to utilize the force of repeated suction to disperse the testicle tissue so as to obtain the free sperm, and the method is long in time consumption; according to the invention, the scheme of combining the fixing device and the poking device is adopted, so that the testicle tissues are relatively fixed in the semen fetching process, the defects that the testicle tissues are easy to slide, cannot be fixed and are inconvenient to fetch semen during semen fetching are overcome, and semen fetching is facilitated; and the poking device can be used for poking the slender seminal tubule efficiently and rapidly, so that the seminal extracting time is shortened, and the seminal extracting efficiency is improved.

Meanwhile, the front end of the injector is provided with a needle point structure, so the structure is easy to accidentally injure the top body area of the sperm head, and the fertilization of the embryo and the development of the subsequent embryo are influenced; the pulling device adopted by the invention is as follows: the tissue extractor 203 is in a hook shape, a U shape, an L shape, a C shape or a ring shape, the L-shaped bending part of the tissue extractor 203 is in a smooth arc structure, and the tail end of the tissue extractor is in a smooth arc structure, so that the structure can effectively avoid the damage to sperms in the extracting process; the sperm free influence caused by sperm factors is reduced, a good foundation is provided for the subsequent pregnancy of the NOA patients, and the re-injury of the NOA patients caused by repeated sperm taking can be avoided;

(2) in the method disclosed in CN105695315A, the scraping needle used in the background art is very vulnerable to sperm damage at the tip, but the sperm in the testis is not mature enough, the nucleus is not fully condensed, the mobility is not good, and the acrosome reaction is insufficient, and the sperm is very vulnerable to external stimulation and located in the acrosome area of the head, which may cause bad influence on embryo fertilization and subsequent embryo development and pregnancy. The invention does not adopt a scraping needle, but uses a tool set to match the fixing and pulling processes, and pulls the fine sperm tube in a pulling mode to ensure that the sperm can fully swim into the washing liquid to obtain the sperm, thereby avoiding the damage to the sperm easily caused in the above documents.

Example 2

The difference from embodiment 1 is that the upper portion of the second connecting rod 102 is also screwed with a bolt to facilitate better fixing of the second connecting rod 102. The rest is the same as in example 1.

Example 3

The difference from embodiment 1 is that the tissue extractor 203 has a "U" shape, and the rest is the same as embodiment 1. The end of the tissue extractor 203 is of a non-pointed structure; the end of the tissue extractor 203 is a rounded arc structure.

Example 4

The difference from the embodiment 1 is that the tissue extractor 203 is in an L shape, the bent part of the L shape is a smooth structure, and the tail end of the tissue extractor 203 is in a non-tip structure; the end of the tissue extractor 203 is a rounded arc structure. The rest is the same as in example 1.

Example 5

The difference from embodiment 1 is that the tissue extractor 203 is in a ring shape, and the end of the tissue extractor 203 is in a non-pointed structure; the end of the tissue extractor 203 is a rounded arc structure. The rest is the same as in example 1.

Example 6

The difference from embodiment 1 is that a handle having a "T" shape is provided at the upper top of the fixing lever 101, and the handle is provided for easy operation.

Example 7

The difference from the embodiment 1 is that the outer surface of the fixing rod 101 is provided with a layer of anti-slip rubber to prevent the fixing rod from accidentally sliding during the operation process to affect the precision taking, thereby improving the stability of the operation.

Example 8

The difference from embodiment 1 is that the upper part of the fixing rod 101 has a recess 110 for the operator to hold the fixing rod 101.

Example 9

The difference from embodiment 1 is that claw-shaped bars are respectively connected to the centers of the triangular sides of the fixing frame 104, and the rest is the same as embodiment 1.

Example 10

In the traditional method, a common injector is used for repeatedly pumping the testicle tissue, the testicle tissue is dispersed by using the repeatedly pumped force so as to obtain the dissociated sperms, the method is long in time consumption, and enough sperms cannot be completely and fully obtained. As shown in figure 1. The tools of the invention are illustrated below by way of example:

congratulating on a certain date, male, age 32; the conventional semen examination of the hospital shows azoospermia for a plurality of times, and the ultrasonography of certain genitals shows testicular microlithography and the karyotype analysis of genetic chromosomes shows no abnormality until the examination of the reproductive center of the applicant (women and children health care institute in Shandong province); after the diagnosis is definite, the right testicle puncture sperms are obtained in the right side of the reproductive center, the sperms are obtained by adopting a conventional syringe repeated suction and syringe tip scraping method in the endoscopic examination in the operation, an inactive sperm is occasionally seen under each high power endoscopic vision field in the endoscopic examination, and a very small amount of spermatids are seen when tissues are sent to the pathology.

In the clinical cases, the procreation department takes tens of cases every year, and the same people in the procreation department always want to solve the similar problem that the sperm is few and easily damaged in the process of taking sperm. According to the operation experience of the special reproductive department for years, the clinical experience of the same kernel of the reproductive department and the joint participation of the same, the invention provides a kit for separating sperms from the extracted testicular tissue. Therefore, a microscopic semen collection was performed for similar cases of me reproductive centers. In the semen collection process, the volume of a testis is about 8ml, a clinician pulls out the surface layer, a second layer and a third layer of relatively good seminiferous tubules are taken and placed in a culture dish containing culture solution and handed to an embryo laboratory, the seminiferous tubules are fixed by a fixing device by adopting the improved semen collection kit, the seminiferous tubules are stripped by a puller on the other side, the sperms can fully swim out of the seminiferous tubules, and the collected sperms are healthy and normal sperms. By adopting the kit provided by the invention to extract semen, the defects that the time consumption is long in the process of extracting semen by adopting an injector and the subsequent normal fertilization and the development of high-quality embryos are influenced are avoided.

A large amount of clinical data show that the kit provided by the invention can be used for extracting semen, so that the semen extracting time can be greatly shortened, and compared with the previous injector for extracting the semen, the time is about 5-10 minutes, the kit can shorten the semen extracting process to 1-2 minutes, and the semen extracting efficiency is improved;

meanwhile, the structure provided by the invention has particularity, so that sperms can be fully protected from being damaged in the sperm taking process, the quality of the taken sperms is greatly improved, and the subsequent fertilization, development and pregnancy of embryos are facilitated.

Example 11

In order to verify the stability of the refining effect of the kit in the using process, the inventor applies the kit to an animal model experiment;

taking a male mouse with the age of 10 weeks, wherein the weight of the male mouse is about 35-40 g; the female mice are 7-8 weeks old and 30-35g in weight, and the male mice and the female mice are bred in cages in an adaptive manner. Injecting PMSG10IU into the abdominal cavity of a female rat, injecting HCG after 48h, and fertilizing 13-14h after HCG injection; the male mice were left untreated.

On the day of fertilization, the female mouse is sacrificed, the oviduct is rapidly stripped and placed in a culture dish filled with HTF culture solution, and the ampulla of the oviduct is torn to enable the cumulus mass to flow out. Oocytes obtained from 10 female mice were placed in a culture dish and cultured until use. Collecting 200 mouse oocytes with good morphology and appearance, arranging 100 mouse oocytes in each group, digesting and removing granular cells for later use in vitro by using a hyaluronic acid enzyme method, wherein 95 mature oocytes are obtained in an experimental group, and 90 mature oocytes are obtained in a control group.

In addition, 10 male mice are sacrificed, testicular tissues are taken, seminal tubules are stripped, a control group (5) and an experimental group (5) are established, the experimental group adopts an improved seminal fetching tool to strip the sperms of the seminal tubules, and the control group adopts a syringe repeated suction method and a syringe tip scraping method to strip the sperms of the seminal tubules.

TABLE 1 mouse sperm extraction test results

As can be seen from the comparison of the data in the table 1, the experimental group adopts the improved semen collection kit, the control group adopts the common syringe for suction and the syringe tip needle for scraping, the kit adopted by the invention is obviously superior to the control group in semen collection time, the number and the activity of sperms are also obviously superior to those of the control group, the fertilization rates between the two groups are compared by chi-square test, the statistical difference is generated (P =0.026 < 0.05), and the blastocyst formation rates between the two groups are compared by chi-square test, the statistical difference is generated (P =0.024 < 0.05); the effect of the experimental group using the improved set of semen extracting tools is obviously better than that of the control group.

Moreover, the kit is used for clinic by repeatedly using the kit, and the finding that the kit can quickly take out sperms without damaging the sperms is realized; has the advantages of short semen collection time, high semen collection efficiency, good semen collection quality and the like.

The structure of the above-mentioned kit is not exhaustive, and other simple modifications made on the basis of the present invention are also within the scope of the present invention.

Claims

1. Kit for obtaining sperm on a seminiferous tubule of a harvested testicular tissue, characterized in that the kit comprises: a fixing device (1) and a dialing device (2);

the fixing device (1) comprises: the device comprises a fixed rod (101), a first connecting rod (102), a second connecting rod (103) and a fixed frame (104);

a first connecting rod (102) is detachably connected below the fixed rod (101); a second connecting rod (103) is connected below the first connecting rod (102), and the second connecting rod (103) is connected with the fixing frame (104);

the mechanism of the pulling device (2) comprises: a tissue plucking rod (201), a third connecting rod (202) and a tissue plucking device (203);

a third connecting rod (202) is detachably connected below the tissue poking rod (201), and a tissue poking device (203) is connected below the third connecting rod (202).

2. The kit for obtaining sperm on extracted testicular tissue seminiferous tubules of claim 1, wherein the fixing frame (104) is any one of an equilateral triangle frame, a cone frame, a rectangular frame, a diamond frame, a circular frame.

3. The kit for obtaining sperm on extracted seminiferous tubules of testicular tissue according to claim 2, wherein the fixation frame (104) is made of medical grade transparent antibacterial plexiglass material.

4. The kit for obtaining sperms on extirpated testicular tissue seminiferous tubules of claim 3, wherein the second connecting rod (103) comprises three extending claw-shaped rods; the angles between adjacent claw-shaped rods are the same.

5. The kit for obtaining sperms on the extracted seminiferous tubules of testicular tissue according to claim 4, wherein the tissue extractor (203) is made of medical transparent antibacterial plexiglass material;

preferably, the appearance structure of the tissue extractor (203) is in a hook shape, a U shape, an L shape, a C shape or a circular shape, and the tail end of the tissue extractor (203) is in a non-tip structure;

preferably, the tail end of the tissue extractor (203) is of a smooth arc structure; or the tissue extractor (203) is provided with a smooth arc structure;

preferably, the tissue extractor (203) is L-shaped, and the L-shaped bending part of the tissue extractor is of a smooth arc structure.

6. The kit for obtaining sperms on extirpated testicular tissue seminiferous tubules of claim 4, wherein the first handle is provided above the fixed rod (101); a second handle is arranged above the tissue poking rod (201);

or the side surface of the fixed rod (101) is provided with a first handle, and the side surface of the tissue poking rod (201) is provided with a second handle;

preferably, the tissue extractor (203) is welded with the third connecting rod (202);

preferably, the tissue extractor (203) and the third connecting rod (202) are in an integral structure;

preferably, the tissue extracting rod (201) and the third connecting rod (202) are connected by clamping or screw threads.

7. The kit for obtaining sperms on extirpated testicular tissue seminiferous tubules of claim 4, wherein the outer surface of the fixed rod (101) and/or the tissue extracting rod (201) is provided with a non-slip rubber layer.

8. The kit for taking sperm from a seminiferous tubule of testicular tissue harvested according to claim 4, wherein in the second connecting rod (103), three claw-shaped rods are connected to three end points of the triangular fixing frame (104), respectively; or the three claw-shaped rods are respectively connected with the middle points of three sides of the triangular fixing frame (104);

preferably, the second connecting rod (103) is welded with the first connecting rod (102);

or the second connecting rod (103) and the first connecting rod (102) are in an integral structure.

9. The kit for obtaining sperms on extirpated seminal tubules of testicular tissue according to claim 1, wherein the first connecting rod (102) is connected with the fixing rod (101) by screw thread;

or the first connecting rod (102) is clamped and connected with the fixed rod (101);

a groove is formed in the lower portion of the fixing rod (101), internal threads are formed in the groove, external threads are formed at the upper end of the first connecting rod (102), and the first connecting rod (102) is connected with the fixing rod (101) through threads;

preferably, a groove is formed in the lower portion of the fixing rod (101), internal threads are formed in the groove, external threads are formed at the upper end of the first connecting rod (102), and the first connecting rod (102) is connected with the fixing rod (101) through threads; a bolt is arranged outside the first connecting rod (102), and the first connecting rod (102) and the fixed rod (101) are further fixed through the bolt.

10. The method of claim 1, wherein the kit for obtaining sperm from the seminiferous tubules of the testicular tissue comprises the steps of:

putting the testicle tissue seminiferous tubule taken out by biopsy into a culture dish filled with buffer solution for cleaning to remove redundant blood cell impurities, transferring the testicle tissue seminiferous tubule into the culture dish containing sperm cleaning solution, setting the temperature of a hot table to be 36.8-37.2 ℃ in an IVF workstation, holding a fixing device (1) in a sleeving tool by a left hand under a stereoscope, fixing the seminiferous tubule by a fixing frame (104) below a fixing rod (101) of the fixing device, taking the seminiferous tubule by a right hand held taking device (2) under a microscope in a visible state by a tissue extractor (203), fully migrating the seminiferous tubule into the cleaning solution under the condition of fully pulling the seminiferous tubule, observing the migration condition of the seminiferous tubules under an inverted microscope, and then recovering and centrifuging for later injection of the single seminiferous tubule in the ootheca for later use, thereby completing the semin.