CN111707811A - Lamb breathing gas concentration detection device and method based on sterile environment - Google Patents
Lamb breathing gas concentration detection device and method based on sterile environment Download PDFInfo
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- 235000019687 Lamb Nutrition 0.000 title claims abstract description 48
- 238000001514 detection method Methods 0.000 title claims abstract description 43
- 230000029058 respiratory gaseous exchange Effects 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title abstract description 9
- 239000007789 gas Substances 0.000 claims abstract description 131
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 60
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 56
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 50
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 30
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 30
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 25
- 239000001301 oxygen Substances 0.000 claims abstract description 25
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 25
- 238000001931 thermography Methods 0.000 claims abstract description 23
- 241000283903 Ovis aries Species 0.000 claims description 43
- 230000003111 delayed effect Effects 0.000 claims description 5
- 150000001335 aliphatic alkanes Chemical class 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 230000007613 environmental effect Effects 0.000 claims description 2
- 230000007306 turnover Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 4
- 230000004060 metabolic process Effects 0.000 description 12
- 238000012544 monitoring process Methods 0.000 description 7
- 230000000241 respiratory effect Effects 0.000 description 5
- 230000029087 digestion Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000036760 body temperature Effects 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 241000283898 Ovis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
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- 230000001737 promoting effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J5/0014—Radiation pyrometry, e.g. infrared or optical thermometry for sensing the radiation from gases, flames
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J5/0022—Radiation pyrometry, e.g. infrared or optical thermometry for sensing the radiation of moving bodies
- G01J5/0025—Living bodies
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0011—Sample conditioning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0062—General constructional details of gas analysers, e.g. portable test equipment concerning the measuring method, e.g. intermittent, or the display, e.g. digital
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0062—General constructional details of gas analysers, e.g. portable test equipment concerning the measuring method, e.g. intermittent, or the display, e.g. digital
- G01N2033/0068—General constructional details of gas analysers, e.g. portable test equipment concerning the measuring method, e.g. intermittent, or the display, e.g. digital using a computer specifically programmed
Abstract
The invention provides a lamb breathing gas concentration detection device based on an aseptic environment, which comprises a sealable aseptic cabin and a gas concentration detection sensor arranged in the aseptic cabin, wherein an access door is arranged on the side wall of the aseptic cabin, the gas concentration detection sensor comprises an oxygen sensor, a carbon dioxide sensor, a methane sensor and a nitrogen sensor, the detection device further comprises computer equipment and an infrared thermal imaging thermometer, the oxygen sensor, the carbon dioxide sensor, the methane sensor, the nitrogen sensor and the infrared thermal imaging thermometer are respectively connected with the computer equipment, the infrared thermal imaging thermometer monitors the gas temperature within 20mm of the peripheries of a lamb mouth and a nose in real time, and transmits the monitored temperature data to the computer equipment; the method can really realize the effect of accurately collecting the content/concentration of the key components in the lamb breathing gas, and has the advantages of high detection efficiency and small stress on the lamb.
Description
Technical Field
The invention relates to a lamb breathing gas concentration detection device and method based on an aseptic environment.
Background
The research of the related basic theory for strengthening the growth and development rules of the lambs is beneficial to improving the cultivation quality of the lambs and further promoting the development of the sheep breeding industry. On one hand, the microorganisms have important regulation and control effects on the growth and development and body health of the lambs, but in the research process, the environment, the matrix, the feed and the like all contain a large amount of microorganisms, so that the experimental data are greatly interfered, and the influence of the microorganisms on the lambs cannot be determined; on the other hand, research on the aspect of nutrient absorption and utilization of lambs can not be carried out with respiratory metabolism and digestive metabolism experiments, when the respiratory metabolism and digestive metabolism experiments are carried out, accurate collection of lamb exhaust is a problem which must be solved by scientific researchers, the respiratory metabolism experiments are carried out in a respiratory chamber in the conventional operation process, but due to the fact that the space of the respiratory chamber is large, gas is incompletely collected, and great experimental errors are caused.
Although the document CN203912874U provides a lamb breathing digestion metabolism cage, which comprises a breathing digestion metabolism cage main body structure, and is in a sealed state during operation, only one air inlet and one air outlet are opened, so that no gas exchange occurs with the outside, the structure is firm and the durability is strong, lambs are put into the metabolism cage by experimenters from a side door of the metabolism cage, the air outlet is connected with a pipeline of a gas component detection device, and gas is pumped away to form a negative pressure chamber in the digestion metabolism cage, so that the air inlet only enters air, and the gas generated by breathing metabolism cannot leak. However, the adoption of the lamb breathing digestion metabolism cage still cannot accurately acquire the content/concentration of key components (such as methane and carbon dioxide) of the lamb breathing gas, and particularly cannot avoid data errors caused by the fact that the gas just exhaled by the lamb is sucked back.
Disclosure of Invention
The invention aims to provide a lamb breathing gas concentration detection device and method based on an aseptic environment, and aims to solve the technical problem that the content/concentration of key components of lamb breathing gas cannot be accurately acquired in the prior art.
In order to achieve the above purpose, the present invention adopts the following technical solutions.
The utility model provides a lamb breathing gas concentration detection device based on sterile environment, includes the aseptic cabin that can seal and sets up the gas concentration detection sensor in aseptic cabin, aseptic cabin lateral wall is provided with business turn over door, its characterized in that:
the gas concentration detection sensor comprises an oxygen sensor, a carbon dioxide sensor, a methane sensor and a nitrogen sensor, the detection device further comprises computer equipment and an infrared thermal imaging thermometer, the oxygen sensor, the carbon dioxide sensor, the methane sensor, the nitrogen sensor and the infrared thermal imaging thermometer are respectively connected with the computer equipment, and the infrared thermal imaging thermometer monitors the gas temperature within 20mm of the peripheries of the mouth and the nose of the lamb in real time and transmits the monitored temperature data to the computer equipment;
the computer device comprises a memory, a processor and a program stored on the memory and executable on the processor, the processor implementing the following steps when executing the program:
step 1, reading gas concentration data fed back by an oxygen sensor, a carbon dioxide sensor, a methane sensor and a nitrogen sensor according to a set frequency;
step 2, calculating t0～t1The average value of oxygen concentration, the average value of carbon dioxide concentration, the average value of methane concentration and the average value of nitrogen concentration in a time period;
step 3, reading t according to the set frequency1Comparing the read temperature and humidity data T with a standard temperature interval according to the temperature and humidity data fed back by the infrared thermal imaging temperature measuring instrument after the moment, and recording the gas concentration A corresponding to 0.5-0.8 second after the temperature and humidity reading moment if the temperature and humidity data T just falls into the standard temperature interval; if the temperature and humidity data T do not fall into a standard temperature interval, wherein the standard temperature interval is a temperature range value of gas just exhaled by the lambs, recording a gas concentration B corresponding to 0.5-0.8 second after the temperature and humidity reading time is delayed;
step 4, calculating and outputting the gas concentrations A and t obtained for the first time0～t1The difference value of the corresponding gas concentration mean value in the time interval is the first expired gas concentration of the lamb;
step 5, calculating and outputting the difference value between the gas concentration B obtained for the first time and the corresponding gas concentration A obtained for the first time, namely the gas concentration inhaled by the lambs for the first time;
step 6, calculating and outputting the difference value between the gas concentration A obtained for the second time and the corresponding gas concentration B obtained for the first time, namely the gas concentration exhaled by the lamb for the second time;
and 7, counting and outputting the concentration mean value of each inhaled gas and the concentration mean value of each exhaled gas until the Nth exhaled and inhaled gas concentration calculation is finished.
Preferably, the set frequency is 0.5 seconds/time.
In order to further improve the accuracy of the detection result, the infrared thermal imaging thermometers are arranged on two side walls and/or the top of the sterile chamber along the length direction, and the shooting range is not less than 3/4 of the length of the inner cavity of the sterile chamber in the length direction.
In order to further improve the accuracy of the detection result and simultaneously reduce the stress to the lambs, the t is0～t1The time interval is 10 seconds after lambs are just put into the sterile chamber and the chamber door is closed, and the total time length of the lambs monitored in the sterile chamber is not more than 30 seconds.
I conveniently monitor the gas temperature at the mouth and the periphery of the nose of the lamb, and the width of the cavity in the sterile capsule is not more than the length of the lamb.
Further, just before the lambs are put into the sterile chamber, the standard environmental temperature in the sterile chamber is controlled to be 20-23 ℃.
As a preferred scheme, three groups of oxygen sensors and three groups of nitrogen sensors are respectively arranged, and six groups of carbon dioxide sensors and six groups of alkane sensors are respectively arranged; in steps 4-7, the gas concentration calculated each time is an average value.
Has the advantages that: by adopting the scheme of the invention, data errors caused by the fact that the gas just exhaled by the lambs is inhaled again can be avoided, the cross influence of the exhaled gas and the inhaled gas can be avoided, the carbon dioxide content and the methane content just exhaled by the lambs and the oxygen content and the nitrogen content inhaled by the lambs can be accurately acquired, the carbon dioxide content and the methane content inhaled together can be accurately detected, and the effect of accurately acquiring the content/concentration of key components in the breathing gas of the lambs is really realized; by adopting the scheme of the invention, the detection efficiency of the content/concentration of the key components in the lamb breathing gas can be greatly improved, under the normal condition, each detection can be completely controlled within one minute (including the time for putting in and taking out the lamb), the detection difficulty of the content/concentration of the key components in the lamb breathing gas can be greatly reduced, the whole detection process is very simple, and the operation can be carried out by one person. In addition, physical interference factors are few by adopting the scheme of the invention, and stress on lambs is hardly caused.
Drawings
FIG. 1 is a schematic view of the lamb breathing gas concentration detection device in a downward direction based on a sterile environment in the embodiment;
FIG. 2 is a schematic view of the lamb breathing gas concentration detection device in a downward direction based on a sterile environment in the embodiment;
in the figure: 1-sterile chamber, 2-gas concentration detection sensor, 3-infrared thermal imaging thermodetector in sterile chamber, 4-lamb body temperature monitoring area in sterile chamber, 5-lamb mouth and nose periphery temperature monitoring area within 20mm in sterile chamber, 6-environment space temperature monitoring area in sterile chamber.
Detailed Description
In the following, the technical solutions of the present invention will be further explained by referring to the following examples, which are not to be construed as limiting the scope of the present invention, and the non-essential modifications and adjustments made by those skilled in the art according to the claims of the present invention are within the scope of the present invention.
Examples
Referring to fig. 1 and 2, a lamb breathing gas concentration detection device based on a sterile environment comprises a closable sterile cabin and a gas concentration detection sensor arranged in the sterile cabin;
wherein the length of the sterile cabin is about 3 times of the body length of the lambs, the width of the sterile cabin is slightly smaller than the body length of the lambs, and the height of the sterile cabin is about 1.5 times of the body length of the lambs; the lateral wall of the sterile cabin is provided with an access door, the access door for lambs to enter the sterile cabin is arranged on the left lateral wall of the sterile cabin shown in the figure 1, and the exit door (outlet) for lambs to leave the sterile cabin is arranged on the right lateral wall of the sterile cabin shown in the figure 1, so that the lambs can conveniently enter and exit in the forward direction by adopting the structure, and the structure is convenient and applicable;
the gas concentration detection sensors comprise three groups of oxygen sensors, three groups of carbon dioxide sensors, six groups of methane sensors and six groups of nitrogen sensors, and all the sensors are alternately and uniformly arranged on the four side walls and the top wall of the sterile cabin;
the detection device also comprises computer equipment and an infrared thermal imaging thermometer, wherein the infrared thermal imaging thermometer adopts a wide-screen structure arranged on the side wall of the sterile cabin so as to ensure that the length direction of a camera shooting range is not less than 3/4 of the length of the inner cavity of the sterile cabin; certainly, an infrared thermal imaging thermometer can also be arranged at the top of the sterile cabin if necessary;
the oxygen sensor, the carbon dioxide sensor, the methane sensor, the nitrogen sensor and the infrared thermal imaging thermometer are respectively connected with computer equipment, the infrared thermal imaging thermometer monitors the gas temperature within 20mm of the mouth and the periphery of the nose of the lamb in real time (namely, the infrared thermal imaging thermometer monitors the temperature in a region 5 shown in a figure 2 in real time, meanwhile, a region 4 in the figure corresponds to the body temperature of the lamb, a region 6 corresponds to the normal environment temperature in the sterile cabin, and in the monitoring process, the regions 4, 5 and 6 respectively present temperature intervals with larger gradients and hardly have the problem of mutual interference), and the monitored temperature data are transmitted to the computer equipment;
the computer device comprises a memory, a processor and a program stored on the memory and executable on the processor, the processor implementing the following steps when executing the program:
step 1, reading gas concentration data fed back by an oxygen sensor, a carbon dioxide sensor, a methane sensor and a nitrogen sensor according to a set frequency; the set frequency is recommended to be 0.5 second/time;
step 2, calculating t0～t1The average value of oxygen concentration, the average value of carbon dioxide concentration, the average value of methane concentration and the average value of nitrogen concentration in a time period; said t is0～t1The time period means that the lambs are just put into the sterile cabin and within 10 seconds after the cabin door is closed;
step 3, reading t according to the set frequency1Comparing the read temperature and humidity data T with a standard temperature interval according to the temperature and humidity data fed back by the infrared thermal imaging temperature measuring instrument after the moment, and recording the gas concentration A corresponding to 0.5-0.8 second after the temperature and humidity reading moment if the temperature and humidity data T just falls into the standard temperature interval; if the temperature and humidity data T do not fall into a standard temperature interval, wherein the standard temperature interval is a temperature range value of gas just exhaled by the lambs, recording a gas concentration B corresponding to 0.5-0.8 second after the temperature and humidity reading time is delayed; in this step, experiments prove that the removal is carried outThe temperature range value of the gas just exhaled by the lamb is preferably 30-34.5 ℃ besides the error caused by the reaction time of the sensor and the computer equipment and the gas heat exchange loss caused by the measurement delay of 0.5-0.8 second;
step 4, calculating and outputting the gas concentrations A and t obtained for the first time0～t1The difference value of the corresponding gas concentration mean value in the time interval is the first expired gas concentration of the lamb;
step 5, calculating and outputting the difference value between the gas concentration B obtained for the first time and the corresponding gas concentration A obtained for the first time, namely the gas concentration inhaled by the lambs for the first time;
step 6, calculating and outputting the difference value between the gas concentration A obtained for the second time and the corresponding gas concentration B obtained for the first time, namely the gas concentration exhaled by the lamb for the second time;
and 7, counting and outputting the concentration mean value of each inhaled gas and the concentration mean value of each exhaled gas until the Nth exhaled and inhaled gas concentration calculation is finished.
The detection method of the lamb breathing gas concentration based on the sterile environment is described in specific cases below.
Placing the sterile cabin in a constant-temperature sterile laboratory with the environment temperature of 20-23 ℃, opening an inlet door of the sterile cabin, closing an outlet door of the sterile cabin, and controlling the placing time to be 8-10 minutes;
setting the total time of monitoring lambs in the sterile chamber to be 28 seconds, setting the data monitoring frequency to be 0.5 second/time, sterilizing the bodies of the lambs aged 2 months, putting the lambs into the sterile chamber, immediately closing an entrance door of the sterile chamber, and simultaneously opening an infrared thermal imaging thermodetector;
within the first 10 seconds (t) after closing the sterile compartment access door0～t1In a time period), a processor of the computer device reads gas concentration data fed back by the oxygen sensor, the carbon dioxide sensor, the methane sensor and the nitrogen sensor according to a set frequency (0.5 second/time), and calculates an oxygen concentration mean value W (Vol%), a carbon dioxide concentration mean value X (Vol%), a methane concentration mean value Y (Vol%) and a nitrogen concentration mean value Z (Vol%) in the time period, wherein the methane concentration mean value Y (Vol%) represents that in 10 secondsThe average value of the methane content exhaled by the lambs each time;
reading temperature and humidity data fed back by an infrared thermal imaging temperature measuring instrument according to a set frequency (0.5 second/time) from the 11 th second after the sterile chamber door is closed, comparing the read temperature and humidity data T with a standard temperature interval (30-34.5 ℃), and recording the gas concentration A corresponding to 0.5-0.8 second after the temperature and humidity reading time is delayed if the temperature and humidity data T just falls into the standard temperature interval which is the temperature range value of the gas just exhaled by the lamb; if the temperature and humidity data T do not fall into the standard temperature range, recording the gas concentration B corresponding to 0.5-0.8 second after the temperature and humidity reading time is delayed;
step 4, calculating and outputting the gas concentrations A and t obtained for the first time0～t1The difference value of the corresponding gas concentration mean value in the time interval is the gas concentration exhaled by the lamb for the first time, wherein the gas concentration A obtained for the first time specifically comprises an oxygen concentration mean value W1 (Vol%), a carbon dioxide concentration mean value X1 (Vol%), a methane concentration mean value Y1 (Vol%) and a nitrogen concentration mean value Z1 (Vol%);
step 5, calculating and outputting the difference value between the gas concentration B obtained for the first time and the corresponding gas concentration A obtained for the first time, namely the gas concentration inhaled by the lambs for the first time; wherein, the first acquired gas concentration B comprises an oxygen concentration mean value W1′(Vol%), mean carbon dioxide concentration X1′(Vol%), mean value of methane concentration Y1′(Vol%), mean nitrogen concentration Z1′(Vol%), the concentration of the first inhalation of the gas by the lambs is the oxygen concentration (W1)′-W), carbon dioxide concentration (X1)′-X), methane concentration (Y1)′-Y), nitrogen concentration (Z1)′-Z)；
Step 6, calculating and outputting the gas concentration A obtained for the second time and the difference value between the gas concentration A obtained for the second time and the corresponding gas concentration B obtained for the first time, namely the gas concentration exhaled by the lamb for the second time; the gas concentration A obtained for the second time specifically comprises an oxygen concentration mean value W2 (Vol%), a carbon dioxide concentration mean value X2 (Vol%), a methane concentration mean value Y2 (Vol%), a nitrogen concentration mean value Z2 (Vol%), and the gas concentrations exhaled for the second time are respectively oxygen concentrations (W2-W)1′) Carbon dioxide concentration (X2-X1)′) Methane concentration (Y2-Y1)′) Nitrogen concentration (Z2-Z1)′)；
And 7, analyzing and calculating according to the rule of sequentially obtaining the gas concentration, and counting and outputting the concentration mean value of each inhaled gas and the concentration mean value of each exhaled gas until the nth exhaled and inhaled gas concentration calculation is finished.
It should be noted that, since multiple sets (multiple sets) of sensors are provided for each sensor, the respective gas concentrations obtained at the same monitoring time refer to the average concentrations obtained by the different sets of sensors, for example, the concentrations obtained by the 1 st, 2 nd and 3 rd sets of carbon dioxide sensors at the 12.8 th second time are 10ppm, 11ppm and 12ppm respectively, then the average carbon dioxide concentration obtained at the detection time is 11ppm, and the output is finally calculated in step 7: each inhaled gas concentration mean value refers to a corresponding gas concentration mean value monitored at all inhalation moments, and each exhaled gas concentration mean value refers to a corresponding gas concentration mean value monitored at all exhalation moments.
By adopting the scheme of the invention, data errors caused by the fact that the gas just exhaled by the lambs is inhaled again can be avoided, the cross influence of the exhaled gas and the inhaled gas can be avoided, the carbon dioxide content and the methane content just exhaled by the lambs and the oxygen content and the nitrogen content inhaled by the lambs can be accurately acquired, the carbon dioxide content and the methane content inhaled together can be accurately detected, and the effect of accurately acquiring the content/concentration of key components in the breathing gas of the lambs is really realized; by adopting the scheme of the invention, the detection efficiency of the content/concentration of the key components in the lamb breathing gas can be greatly improved, under the normal condition, each detection can be completely controlled within one minute (including the time for putting in and taking out the lamb), the detection difficulty of the content/concentration of the key components in the lamb breathing gas can be greatly reduced, the whole detection process is very simple, and the operation can be carried out by one person. In addition, physical interference factors are few by adopting the scheme of the invention, and stress on lambs is hardly caused.
Claims (7)
1. The utility model provides a lamb breathing gas concentration detection device based on sterile environment, includes the aseptic cabin that can seal and sets up the gas concentration detection sensor in aseptic cabin, aseptic cabin lateral wall is provided with business turn over door, its characterized in that:
the gas concentration detection sensor comprises an oxygen sensor, a carbon dioxide sensor, a methane sensor and a nitrogen sensor, the detection device further comprises computer equipment and an infrared thermal imaging thermometer, the oxygen sensor, the carbon dioxide sensor, the methane sensor, the nitrogen sensor and the infrared thermal imaging thermometer are respectively connected with the computer equipment, and the infrared thermal imaging thermometer monitors the gas temperature within 20mm of the peripheries of the mouth and the nose of the lamb in real time and transmits the monitored temperature data to the computer equipment;
the computer device comprises a memory, a processor and a program stored on the memory and executable on the processor, the processor implementing the following steps when executing the program:
step 1, reading gas concentration data fed back by an oxygen sensor, a carbon dioxide sensor, a methane sensor and a nitrogen sensor according to a set frequency;
step 2, calculating t0～t1The average value of oxygen concentration, the average value of carbon dioxide concentration, the average value of methane concentration and the average value of nitrogen concentration in a time period;
step 3, reading t according to the set frequency1Comparing the read temperature and humidity data T with a standard temperature interval according to the temperature and humidity data fed back by the infrared thermal imaging temperature measuring instrument after the moment, and recording the gas concentration A corresponding to 0.5-0.8 second after the temperature and humidity reading moment if the temperature and humidity data T just falls into the standard temperature interval; if the temperature and humidity data T do not fall into a standard temperature interval, wherein the standard temperature interval is a temperature range value of gas just exhaled by the lambs, recording a gas concentration B corresponding to 0.5-0.8 second after the temperature and humidity reading time is delayed;
step 4, calculating and outputting the gas concentrations A and t obtained for the first time0～t1The difference value of the corresponding gas concentration mean value in the time interval is the first expired gas concentration of the lamb;
step 5, calculating and outputting the difference value between the gas concentration B obtained for the first time and the corresponding gas concentration A obtained for the first time, namely the gas concentration inhaled by the lambs for the first time;
step 6, calculating and outputting the difference value between the gas concentration A obtained for the second time and the corresponding gas concentration B obtained for the first time, namely the gas concentration exhaled by the lamb for the second time;
and 7, counting and outputting the concentration mean value of each inhaled gas and the concentration mean value of each exhaled gas until the Nth exhaled and inhaled gas concentration calculation is finished.
2. The lamb breathing gas concentration detection device according to claim 1, wherein: the set frequency was 0.5 seconds/time.
3. The lamb breathing gas concentration detection device according to claim 1 or 2, wherein: the infrared thermal imaging thermometers are arranged on two side walls and/or the top of the sterile cabin along the length direction, and the image pickup range is not less than 3/4 of the length of the inner cavity of the sterile cabin in the length direction.
4. The lamb breathing gas concentration detection device of claim 3, wherein: said t is0～t1The time interval is 10 seconds after lambs are just put into the sterile chamber and the chamber door is closed, and the total time length of the lambs monitored in the sterile chamber is not more than 30 seconds.
5. The lamb breathing gas concentration detection device of claim 4, wherein: the width of the cavity in the sterile capsule is not more than the body length of the lambs.
6. The lamb breathing gas concentration detection device of claim 5, wherein: just before lambs are put into the sterile chamber, the standard environmental temperature in the sterile chamber is controlled to be 20-23 ℃.
7. The lamb breathing gas concentration detection device of claim 6, wherein: the oxygen sensor and the nitrogen sensor are respectively provided with three groups, and the carbon dioxide sensor and the alkane sensor are respectively provided with six groups; in steps 4-7, the gas concentration calculated each time is an average value.
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