CN111707626B - Stabilizer for tyrosine in urine and application thereof - Google Patents
Stabilizer for tyrosine in urine and application thereof Download PDFInfo
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- CN111707626B CN111707626B CN202010558029.7A CN202010558029A CN111707626B CN 111707626 B CN111707626 B CN 111707626B CN 202010558029 A CN202010558029 A CN 202010558029A CN 111707626 B CN111707626 B CN 111707626B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
A stabilizer of tyrosine in urine and application thereof belong to the field of in vitro diagnostic reagents. The stabilizer consists of magnesium sulfate, polyvinylpyrrolidone and sodium dehydroacetate, wherein the mass concentration of each component is as follows: 0.1 to 5g/L of magnesium sulfate, 0.1 to 2.2g/L of sodium dehydroacetate and 0.5 to 3g/L of polyvinylpyrrolidone. The stabilizing agent for detecting the tyrosine content in the urine can obviously prolong the stabilizing time of the tyrosine in the urine, has simple operation, accurate result and good stability, and plays a good role in protecting the detection stability and blank background of the tyrosine in the urine.
Description
Technical Field
The invention belongs to the field of in-vitro diagnostic reagents, and particularly relates to a stabilizer for tyrosine in urine and application thereof.
Background
The tyrosine content in human urine is one of the important indicators of human amino acid metabolism. Practice proves that when human body is in tumor occurrence, amino acid can be changed in different degrees and different types of metabolism, and tyrosine in blood or urine is changed. At present, the content of tyrosine is detected by the following method: the qualitative nitric acid method, the qualitative hydroxyl color development colorimetric method, the oxygen electrode method, the enzyme coupling method, the high performance liquid chromatography method and the like. However, whatever method is used, the first morning urine middle section of the day when the detected person stays is required to be suitable for the specimen, the residence sample and the hospital sample have a time problem, the time is long or short when the detected person is sent to the front of the detected person due to various reasons, the condition that the time is longer than 4 hours often occurs, and the test shows that the tyrosine in the urine for a long time is changed to different degrees at normal temperature, so that the detection accuracy is required to be ensured, and the stability of the tyrosine in the urine is required to be ensured to be good first.
Disclosure of Invention
Aiming at the defects, the invention provides the stabilizer for the tyrosine in the urine and the application thereof, and the stabilizer ensures that the tyrosine content in the urine has good stability within a certain time range. Is favorable for accurately detecting the tyrosine content of urine.
The stabilizer adopted for solving the technical problems comprises magnesium sulfate, polyvinylpyrrolidone and sodium dehydroacetate, and the mass concentration of each component is as follows: 0.1-5 g/L of magnesium sulfate, 0.1-2.2 g/L of sodium dehydroacetate, 0.5-3 g/L of polyvinylpyrrolidone, distilled water as solvent and 5.3-8.2 of pH value of stabilizer.
The invention also discloses an application method of the stabilizing agent, wherein 0.1ml of the stabilizing agent is added into 10ml of urine.
Principle of: the magnesium sulfate in the stabilizer has the effect of reducing blank background, and can protect some groups from oxidation. The polyvinylpyrrolidone plays a role in stabilizing urine, so that the preparation solution has better solubility and storage stability, and the polyvinylpyrrolidone is a nonionic high molecular compound, has a molecular structure similar to a simple protein structure and has good water solubility. The preservative sodium dehydroacetate prevents bacteria from growing and reproducing.
The beneficial effects are that: the stabilizing agent for detecting the tyrosine content in urine can obviously prolong the stabilizing time of tyrosine in urine, has the effective period of 72 hours at normal temperature, 45 days at 2-8 ℃ and 6 months at-20 ℃ and 12 months at-80 ℃ respectively, and obviously ensures that blank absorbance values of specimens do not have interference influence. The operation is simple, the result is accurate, the stability is good, and the detection stability and blank background of tyrosine in urine are well protected.
Detailed Description
The invention is further illustrated below with reference to examples.
Example 1
0.5g of magnesium sulfate, 2.2g of sodium dehydroacetate and 1.5g of polyvinylpyrrolidone are weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 2
0.1g of magnesium sulfate, 1.5g of sodium dehydroacetate and 1g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 3
5g of magnesium sulfate, 0.1g of sodium dehydroacetate and 0.5g of polyvinylpyrrolidone are weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 4
0.7g of magnesium sulfate, 1.6g of sodium dehydroacetate and 2g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 5
3.5g of magnesium sulfate, 1.7g of sodium dehydroacetate and 2.2g of polyvinylpyrrolidone are weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 6
4.3g of magnesium sulfate, 0.2g of sodium dehydroacetate and 3g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 7
2.4g of magnesium sulfate, 0.7g of sodium dehydroacetate and 2g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 8
2.8g of magnesium sulfate, 0.5g of sodium dehydroacetate and 2.2g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 9
0.9g of magnesium sulfate, 2.0g of sodium dehydroacetate and 2.8g of polyvinylpyrrolidone are weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 10
1.6g of magnesium sulfate, 0.9g of sodium dehydroacetate and 1.3g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 11
1.3g of magnesium sulfate, 1.1g of sodium dehydroacetate and 1.9g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
Example 12
3.6g of magnesium sulfate, 0.2g of sodium dehydroacetate and 0.8g of polyvinylpyrrolidone were weighed and dissolved in 1000ml of distilled water to obtain a stabilizer.
80ml of the newly collected sample 1 urine sample was taken, wherein 40ml of the newly collected sample 1 urine sample was evenly divided into four groups after 0.4ml of the stabilizing agent prepared in example 1 was added, the first group was left at normal temperature for 72 hours, the second group was kept at 4-8℃for 45 days, the third group was kept at-20℃for 180 days, and the fourth group was kept at-80℃for 360 days, and 10ml of sample 1 urine was set as a control group for each group.
80ml of the newly collected sample 2 urine sample was taken, wherein 40ml of the newly collected sample 2 urine sample was evenly divided into four groups after 0.4ml of the stabilizing agent prepared in example 1 was added, the first group was left at normal temperature for 72 hours, the second group was kept at 4-8℃for 45 days, the third group was kept at-20℃for 180 days, and the fourth group was kept at-80℃for 360 days, and 10ml of sample 1 urine was set as a control group for each group.
In addition, standard liquid is also provided as a control.
The stability experiment is carried out by adopting the reagent of the enzyme-linked immunosorbent assay, the tyrosine content of the sample is detected by using an enzyme-linked immunosorbent assay, and the result is shown in the following table:
urine tyrosine measuring meter under different preservation temperatures
Remarks: all the above data are averages of 10 measurements made on the same sample.
From the results in the table, the stabilizer has good effect and meets the urine preservation requirement of tyrosine detection.
Analysis of results:
the above table shows that the test data only show good stability initially, with a large deflection at normal temperature at termination, a small deflection at-20℃and almost no deflection at-80℃compared with the two specimens without the stabilizer. The sample added with the stabilizer is still very stable in detection result after being placed under the specified storage condition, and the relative deviation is less than 4%, so that the accuracy is good. The specimen detection service life is remarkably prolonged.
The above-described embodiments are provided for illustration and description of the present invention only and are not intended to limit the invention to the embodiments described. In addition, those skilled in the art will appreciate that the present invention is not limited to the embodiments described above, and that many variations and modifications are possible in light of the teachings of the invention, which variations and modifications are within the scope of the invention as claimed.
Claims (4)
1. The stabilizer for tyrosine in urine is characterized by comprising magnesium sulfate, polyvinylpyrrolidone and sodium dehydroacetate, wherein the mass concentration of each component is as follows: magnesium sulfate 0.5g/L, sodium dehydroacetate 2.2g/L, polyvinylpyrrolidone 1.5g/L.
2. The stabilizer for tyrosine in urine according to claim 1, wherein the solvent is distilled water.
3. The stabilizer for tyrosine in urine according to claim 1, wherein the pH of the stabilizer is 5.3-8.2.
4. A method of using the stabilizer of claim 1, wherein 0.1ml of stabilizer is added per 10ml of urine.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010558029.7A CN111707626B (en) | 2020-06-18 | 2020-06-18 | Stabilizer for tyrosine in urine and application thereof |
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| CN202010558029.7A CN111707626B (en) | 2020-06-18 | 2020-06-18 | Stabilizer for tyrosine in urine and application thereof |
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| CN111707626A CN111707626A (en) | 2020-09-25 |
| CN111707626B true CN111707626B (en) | 2023-09-01 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318597A (en) * | 2011-08-19 | 2012-01-18 | 张雪云 | Liquid-based cell preservation liquid composite and preparation method thereof |
| CN104422688A (en) * | 2013-08-21 | 2015-03-18 | 中国科学院宁波材料技术与工程研究所 | Cysteine detection reagent and detection method thereof |
| CN106244672A (en) * | 2016-08-19 | 2016-12-21 | 威海威仕泰医疗科技有限公司 | A kind of stabilizer for uric acid detectable |
| CN107881213A (en) * | 2017-11-10 | 2018-04-06 | 人和未来生物科技（长沙）有限公司 | A kind of urine preserves reagent and its application |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060014214A1 (en) * | 2004-05-25 | 2006-01-19 | Sierra Diagnostics, Llc | Urine preservation system |
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- 2020-06-18 CN CN202010558029.7A patent/CN111707626B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318597A (en) * | 2011-08-19 | 2012-01-18 | 张雪云 | Liquid-based cell preservation liquid composite and preparation method thereof |
| CN104422688A (en) * | 2013-08-21 | 2015-03-18 | 中国科学院宁波材料技术与工程研究所 | Cysteine detection reagent and detection method thereof |
| CN106244672A (en) * | 2016-08-19 | 2016-12-21 | 威海威仕泰医疗科技有限公司 | A kind of stabilizer for uric acid detectable |
| CN107881213A (en) * | 2017-11-10 | 2018-04-06 | 人和未来生物科技（长沙）有限公司 | A kind of urine preserves reagent and its application |
Non-Patent Citations (1)
| Title |
|---|
| 柳文媛 主编.药物分析进展（第二版）.《药物分析进展（第二版）》.江苏凤凰科学技术出版社,2018,第222页. * |
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