CN111680266A - Method for measuring and calculating equivalent dispersion density of marine floating macroalgae - Google Patents
Method for measuring and calculating equivalent dispersion density of marine floating macroalgae Download PDFInfo
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Abstract
The invention discloses a method for measuring and calculating equivalent dispersion density of marine floating macroalgae, which comprises the following steps: carrying out an experiment of the influence of the environmental restriction factor on the growth of the macroalgae; determining a limiting effective supply volume of the environmental restriction factor and a floating macroalgae theoretical limiting equivalent dispersion density; calculating an environmental restriction factor standard limit effective supply area and a standard limit effective supply radius; calculating an apparent effective feed zone area of the environmental restriction factor; calculating the equivalent dispersion density of floating macroalgae under the condition of environmental restriction factor. The method is beneficial to early comprehensive prevention and control of the large-scale algal blooms and promotion of major marine disaster coping and marine environment safety guarantee capability.
Description
Technical Field
The invention belongs to the field of marine ecology, and particularly relates to a method for measuring and calculating the equivalent dispersion density of algae.
Background
Since the last 70 s, the scale and harm of harmful green tide and gold tide become more and more serious, and the large-scale outbreak of enteromorpha is encountered in the Bordea, France, Korea, Japanese coastal and the like, which brings serious influence on the quality of seawater and the development of coastal economy. Since 2007, the south yellow sea of China continuously outbreaks the largest-scale enteromorpha green tide disaster in the world, and 2017 simultaneously generates green tide and golden tide, which brings serious influence on the ecological environment, marine organism diversity, fishery culture and coastal city tourism of the south yellow sea. Seawater eutrophication is generally considered to be one of the material bases and important influencing factors causing the occurrence of harmful large algal blooms such as green tides, gold tides and the like. However, no better measures are available at present for preventing ecological disasters such as green tide, golden tide and the like, and emergency salvage is still the main means for dealing with the large algal blooms.
The biomass of the large algae is an important parameter for starting salvage and carrying out disaster reduction and prevention. However, floating macroalgae are not homogeneously dispersed in seawater and cannot be strictly described by using the concept of "concentration", "density", etc. to describe a homogeneous dispersion. How to obtain floating macroalgae biomass is always a difficult problem which is troubling academic circles and management departments at present.
Under certain marine environmental conditions, the 'equivalent dispersion density' of floating macroalgae is calculated, the existing biomass of the macroalgae is calculated by combining the coverage area and the environmental restriction factor, and the future biomass of the macroalgae is predicted, so that the method has important significance for preventing occurrence of green tide, golden tide and other macroalgae blooms.
Although the remote sensing inversion technology can be successfully applied to estimation of the coverage area, the distribution area and the change of the coverage area and the distribution area along with the time of floating green algae, the floating large algae biomass serving as an important parameter for estimating the large algae disaster scale and making disaster prevention and reduction decisions cannot be accurately estimated by the existing remote sensing method, and one of the technical bottlenecks of the remote sensing inversion technology is that the dispersion density of the floating large algae cannot be accurately measured and calculated. Although research on the estimation of macroalgae biomass through a hydrodynamic-ecological dynamic coupling model is also available, the model has many parameters and is difficult to verify through experiments.
At present, no relevant report for accurately measuring and calculating the equivalent dispersion density of floating macroalgae exists.
Disclosure of Invention
In order to solve the technical problems mentioned in the background art, the invention provides a method for measuring and calculating the equivalent dispersion density of marine floating macroalgae.
In order to achieve the technical purpose, the technical scheme of the invention is as follows:
a method for measuring and calculating equivalent dispersion density of marine floating macroalgae comprises the following steps:
(1) carrying out an experiment of the influence of the environmental restriction factor on the growth of the macroalgae;
(2) determining the limit effective supply volume of the environmental restriction factor and the theoretical limit equivalent dispersion density of the floating macroalgae based on the experimental result of the step (1);
(3) calculating an environmental restriction factor standard limit effective supply area and a standard limit effective supply radius;
(4) calculating the coverage area of the macroalgae plaque by using remote sensing image data and graphic analysis software, and calculating the apparent effective supply area of the environmental restriction factor by taking the standard limit effective supply radius calculated in the step (3) as a search distance;
(5) calculating the equivalent dispersion density of floating macroalgae under the condition of environmental restriction factor.
Further, in the step (1), offshore macroalgae are collected, healthy algae are selected, surface attachments are removed, the algae are repeatedly washed by filtered natural seawater for multiple times, the washed algae are placed in a refrigerator for temporary storage, then culture experiments are carried out under the environmental condition controlled by an illumination incubator, the algae are taken out after an illumination period, the moisture on the surface of the algae is absorbed, the wet weight of the algae is weighed, the volume of the algae is measured, and the level change of a limiting factor is measured after culture solution is filtered.
Further, seawater was filtered through a 0.45 μm mixed fiber filter, stored in a polyethylene tank, sterilized by a steam sterilizer at 120 ℃ for 15 minutes before the experiment, naturally cooled, and sufficiently shaken to recover the original amount of dissolved gas, thereby obtaining a culture solution for the culture experiment.
Furthermore, when the culture experiment is carried out, the moisture on the surfaces of the algae is firstly absorbed, weighed, the algae with the same mass is placed in a plurality of containers, filtered, boiled and cooled culture solution with different volumes is placed in each container, nutrient salt is added to ensure that the limiting factor levels of the containers are the same, and then the containers are placed in an illumination incubator for culture.
Further, in the step (2), the change Δ C of the level of the limiting factor before and after the light cycle under the condition of different volumes of the culture solution is calculated, the Δ C under the condition of different volumes of the culture solution constitutes a distribution, and the distribution is calculatedThe volume corresponding to Δ C when the percentile is 5, the limit effective supply volume V as the environmental restriction factorTDividing the cultivation mass m of the macroalgae by the limit effective supply volume VTObtaining the theoretical limit equivalent dispersion density D of the floating macroalgaeT。
Further, in step (3), the environmental restriction factor standard limit effective supply radius rTMCalculated as follows:
environmental restriction factor standard limit effective supply area STMCalculated as follows:
in the above formula, VTMIs the ultimate effective feed volume per mass of macroalgae.
Further, in step (4), the macroalgae plaque coverage area S is calculated by the analysis of the graphic analysis software using the remote sensing image datacEffective supply radius scale r according to standard limitTMCalculating an apparent limiting effective supply radius R of macroalgae plaquesaWith RaAs the aggregation distance, scattered macroalgae patches are combined into a plurality of synthetic patches having a larger area by aggregation analysis, and the apparent effective supply region area S of each synthetic patch is calculatedwakS of each synthetic plaquewakSumming as an environmental limiting factor apparent effective supply area Swa。
Further, the apparent limit effective feed radius RaCalculated as follows:
in the above formula, SrThe minimum resolution area of the remote sensing image is obtained.
Further, in the step (5), the floating is made largeAlgal equivalent Dispersion Density Dequ＝DTObtaining the theoretical limit effective supply area S of the environmental restriction factorwT：
In the above formula, ρhtIs the own density of the macroalgae, rhowThe density of the culture broth itself, h1Statistical Length of Floating macroalgae, h2Depth of water h providing a limiting factor for floating macroalgae growth2＝h1+rTM；
When S iswa≤SwTThen:
when S iswa>SwTThen:
Dequ＝DT。
adopt the beneficial effect that above-mentioned technical scheme brought:
according to the method, based on the experimental result of the influence of the environmental restriction factor on the growth of the macroalgae, the remote sensing image data is combined, the coverage area of the floating macroalgae and the apparent effective supply area of the restriction factor are calculated through analysis, the equivalent dispersion density of the floating macroalgae in a certain water area in the marine environment is calculated, the technical bottleneck of the existing biomass calculation of the floating macroalgae is broken through, the problem that the algal bloom biomass of the floating macroalgae is difficult to estimate is solved to a certain extent, and a decision basis is provided for starting the salvaging of the macroalgae and developing disaster reduction and prevention actions. In addition, by combining a macroalgae growth model and utilizing the equivalent dispersion density, the development trend of the future macroalgae biomass can be more accurately predicted, and the environmental protection department can determine the scale and time of salvage actions according to the development trend, thereby being beneficial to improving the disaster prevention and reduction capability of related departments.
Drawings
FIG. 1 is a flow chart of a method of the present invention;
FIG. 2 is a schematic view of the environmental restriction factor limit effective supply volume for large floating algae growth;
FIG. 3 is a schematic view of an effective supply area of large floating algae patches and a polymerization process.
Detailed Description
The technical scheme of the invention is explained in detail in the following with the accompanying drawings.
As shown in figure 1, the invention designs a method for measuring and calculating the equivalent dispersion density of marine floating macroalgae, which comprises the following steps:
step 1: carrying out an experiment of the influence of the environmental restriction factor on the growth of the macroalgae;
step 2: determining the limit effective supply volume of the environmental restriction factor and the theoretical limit equivalent dispersion density of the floating macroalgae based on the experimental result of the step 1;
and step 3: calculating an environmental restriction factor standard limit effective supply area and a standard limit effective supply radius;
and 4, step 4: calculating the coverage area of the macroalgae plaque by using remote sensing image data and graphic analysis software, and calculating the apparent effective supply area of the environmental restriction factor by taking the standard limit effective supply radius calculated in the step 3 as a search distance;
and 5: calculating the equivalent dispersion density of floating macroalgae under the condition of environmental restriction factor.
The following describes a specific implementation process of the present invention by using specific embodiments.
1) Experiment of influence of limiting factor level (nitrate concentration) on growth of macroalgae (enteromorpha)
Collecting Enteromorpha prolifera
Collecting sea grass near sea, selecting healthy algae, removing surface attachments, repeatedly cleaning with filtered natural seawater for several times, placing cleaned algae in a sealed box containing seawater, storing in a refrigerator, and taking back to laboratory.
② enlargement culture
Seawater for culture experiment is collected from yellow sea, filtered with 0.45 μm mixed fiber filter membrane, stored in polyethylene barrel (5L ground bottle), sterilized with steam sterilizer at 125 deg.C for 15min before experiment, naturally cooled, and sufficiently oscillated to recover original dissolved gas amount.
The culture solution is prepared by filtering high-pressure steam to sterilize natural seawater and adding nutrient salt according to the f/2 formula.
Selecting and separating an enteromorpha sample in a laboratory, selecting healthy algae, soaking in 0.2% KI solution for 1-3min for sterilization, washing for 3-4 times by filtered high-temperature sterilized seawater (mixed fiber filter membrane, sterilization at 125 ℃ for 15min), placing in a container containing 1L of culture solution, placing in an illumination incubator for expanded culture at the temperature of 15 +/-1 ℃, the illumination intensity of 6000lux, and the illumination period of L: d is 12 h: and (4) 12 h. The culture medium was changed every 3 days.
(iii) cultivation experiment
Measuring 14L of autoclaved seawater, putting the autoclaved seawater into a 25L polyethylene barrel, adding the nitrogen and phosphorus nutrient salt concentration according to the table 1, uniformly stirring, measuring corresponding volumes, and putting the measured volumes into 0.5L, 1.0L, 2.0L and 2.5L beakers respectively, wherein each group comprises 2 parts for later use. The rest culture solution is used for measuring the concentration of nitrogen and phosphorus nutrient salts (the concentration of nitrogen and phosphorus is measured in the culture solution).
Taking the enteromorpha subjected to nitrogen starvation culture for 72 hours out of the culture bottle, sucking the water on the surface of the algae by using absorbent paper, weighing 0.2 g/part of enteromorpha by using an electronic balance, washing by using sterilized seawater, and placing in beakers containing culture solution, wherein each group comprises 2 parallel samples.
Culturing enteromorpha in an illumination incubator, wherein the temperature is 20 +/-1 ℃, the illumination intensity is 6000lux, and the illumination period is 12 h: the culture period is 1d for 12 h. Inoculating for 24h, taking out Enteromorpha prolifera from the culture beaker, sucking dry surface water of the thallus with filter paper, weighing the wet weight and measuring the volume, filtering the culture solution with 0.45 μm mixed fiber filter membrane, and measuring NaNO3And (4) taking another 100mL of filtered culture solution, placing the filtered culture solution into a sample bottle, and freezing and storing at the temperature of minus 20 ℃.
TABLE 1 culture volume (L) and nutrient salt concentration (. mu.mol L)-1)
| Numbering | V | NaNO3 | NaH2PO4 |
| C1# | 0.1 | 10 | 0.5 |
| C2# | 0.3 | 10 | 0.5 |
| C3# | 0.5 | 10 | 0.5 |
| C4# | 0.7 | 10 | 0.5 |
| C5# | 1.0 | 10 | 0.5 |
| C6# | 1.5 | 10 | 0.5 |
| C7# | 2.0 | 10 | 0.5 |
2) Nitrate (NaNO)3) Determination of ultimate effective supply volume and enteromorpha (theoretical) ultimate equivalent dispersion density
Based on the experimental results of the influence of the nitrate concentration on the growth of enteromorpha, the change (delta C) of the nitrate concentration before and after one photoperiod culture under different volume culture solution conditions is calculated, the delta Ci under different volume culture solution conditions forms a distribution, see table 2 and figure 2, and the volume corresponding to the delta C when the percentile of the distribution is 5 (5%), namely the ultimate effective supply volume (V) is calculatedT) It was 1.85L. Dividing the enteromorpha culture mass of 0.2g by the limit effective supply volume to obtain the (theoretical) limit equivalent dispersion density (D)T) Is 0.11g L-1. The ultimate effective supply volume (V) of Enteromorpha prolifera per unit massTM) Is 9.25L g-1. Wherein, CbAt the level of the pre-photoperiod limiting factor, CaIs the level of the post-photoperiod limiting factor.
TABLE 2 culture solution volume (L) and concentration difference of nitrate before and after photoperiod culture (. mu. mol L)-1)
| Numbering | V | Ca | ΔCi |
| C1# | 0.1 | 0.2 | 9.8 |
| C2# | 0.3 | 0.4 | 9.6 |
| C3# | 0.5 | 1.2 | 8.8 |
| C4# | 0.7 | 2.5 | 7.5 |
| C5# | 1.0 | 6.4 | 3.6 |
| C6# | 1.5 | 8.9 | 1.1 |
| C7# | 2.0 | 9.8 | 0.2 |
And (4) analyzing results: the actual sea area has 24-hour photoperiod and 24-hour tide period, the seawater exchange is more sufficient, the culture experiment obtains the corresponding relation between the culture solution volume and the nitrate concentration through the determination of the nitrate absorption rateA concentration variation distribution is constructed from the concentration differences of a series of culture volumes, a percentile (5% acceptable error) close to the measurement error is calculated, and the corresponding nitrate limiting effective supply volume (V) is calculatedT) And further obtaining a (theoretical) ultimate equivalent dispersion density (D)T) And a standard limit effective supply volume (V)TM)。
3) Determination of standard limit effective supply area and standard limit effective supply radius of enteromorpha growth limiting factor (nitrate)
Effective supply of volume (V) from standard limitsTM) Calculating the standard limit effective supply radius (r) of nitrateTM) Is 0.164m g-1Standard limit effective supply area (S)TM) Is 0.0846m2g-1. Statistical length (h) of floating enteromorpha1) Generally 0.17 +/-0.06 m, and correspondingly can provide the water depth of nitrate for the growth of enteromorpha, namely the depth (h) of an effective nitrate supply area2) Is 0.33 m.
And (4) analyzing results: standard limit effective supply area (S)TM) And a standard limit effective supply radius scale (r)TM) Is used for describing the maximum reach range of the unit mass of enteromorpha for absorbing and utilizing nitrate in a free floating state in a photoperiod. In the early growth stage of enteromorpha, the enteromorpha is few in quantity and sparse in distribution, and the distance between different enteromorpha plaques is greater than a standard limit effective supply radius standard (r)TM) (ii) a In the middle and later growth period of the enteromorpha, the enteromorpha is large in quantity, very densely distributed and even stacked, and the distance between different enteromorpha plaques is possibly smaller than the standard limit effective supply radius standard (r)TM)。
4) Determination of effective supply area of limiting factor (nitrate) during enteromorpha growth
Utilizing HJ-1A/1B (CCD) remote sensing image data (pixel element 30m × 30m), and calculating the coverage area (S) of enteromorpha prolifera 5, 16 and 2012 by the statistical analysis function of ArcGIS softwarec) Is 3.5 × 106m2(ii) a Effective radius of feed (r) according to standard limitTM＝0.164m g-1) Calculating the polymerization distance (apparent ultimate effective feed radius R)a) 1746.1m, scattered enteromorpha is subjected to cluster analysis by the softwareThe plaque-covered patches are combined into a plurality of synthetic patches having a larger area, and the apparent effective supply area of nitrate (S) of each synthetic patch is calculatedwak) The sum of the areas is the apparent effective supply area (S) of the nitratewa) Is 14.6 × 106m2。
And (4) analyzing results: scattered enteromorpha prolifera coverage patches are different in size and distance, and the distance between some patches is smaller than the polymerization distance (R)a) Some greater than the polymerization distance (R)a). All by spacings less than the polymerization distance (R)a) The plaque of (a) is polymerized to obtain a continuous void-free "synthetic plaque" having an effective nitrate supply area that is less than the sum of the effective nitrate supply areas of the respective plaques that are polymerized. If there are individual adjacent patches in the delivery area that are spaced more than the aggregate distance (R)a) There are cases where voids occur in the supply zone, the spacing being much greater than the polymerization distance (R)a) Usually do not aggregate, see FIG. 3, where Sci: macroalgae patch i (i ═ 1 to n) coverage area; swi: the effective supply area of the restriction factor for the macroalgae patch i (i ═ 1 to n); swak: the restriction factor is the apparent effective supply area of the polymerized macroalgae synthetic plaque k (k 1 to m).
5) Calculation of equivalent dispersion density of floating enteromorpha
Defining the equivalent Dispersion Density D of Floating macroalgaeequComprises the following steps:
wherein M ishtIs the mass of macroalgae, VwTo limit the factor effective supply volume, ScCoverage area of macroalgae, SwLimiting the effective supply area of the factor, phtIs the own density of the macroalgae, rhowSea water (culture broth) density, h1Statistical Length of Floating macroalgae, h2Depth of water providing a limiting factor for floating macroalgae growth, i.e. depth of effective zone for providing limiting factor。
Calculating the nitrate Limit effective feed area (S)wT) Is 16.2 × 106m2。
Due to Swa≤SwTEquivalent dispersion density D of floating enteromorphaequComprises the following steps:
6) measurement and verification of equivalent dispersion density of the floating enteromorpha prolifera:
the existing biomass of the enteromorpha can be determined by fishing the floating enteromorpha in a sea area with a certain area, measuring the biomass of the floating enteromorpha, and multiplying the biomass by the enteromorpha coverage area obtained by remote sensing data. The enteromorpha equivalent dispersion density multiplied by the limiting factor apparent effective feeding area (S) measured by the method of the inventionwa) And the depth of the effective supply area of the limiting factor can be used for obtaining the existing biomass of the enteromorpha. And comparing whether the measurement result of the method is significantly different from the result of the field salvage measurement, so that the reliability of the equivalent dispersion density result determined by the method can be verified.
1.37 × 10 biomass of enteromorpha is estimated by salvaging from 5 months to 14 days to 22 days in 20128kg (Liu et al, 2015), determining the equivalent dispersion density D of floating enteromorpha using the method of the inventionequMultiplied by the apparent effective nitrate supply area (S)wa) Obtaining the corresponding enteromorpha biomass 6.0 × 108kg, compared with the salvage estimation method, the magnitude order is the same, and the two are basically consistent.
As the actual sea area free floating enteromorpha coverage thickness is basically consistent, the green tide burst scale actual survey data of the enteromorpha represents biomass by coverage area, and the actual sea area enteromorpha coverage area is increased by multiple (S)t/S0) The equivalent dispersion density increase factor (D) calculated by the model under the same time conditionequ_t/Dequ_0) And comparing, and verifying that the calculation result of the test model has no significant difference from the result of the field measurement, thereby verifying the reliability of the calculation result of the equivalent dispersion density determined by the method.
According to 2012The results of the field survey from 4 to 6 in the middle of the month show that the average nitrate concentration is 9.1. mu. molL-1The coverage area of the enteromorpha is increased by 13.7 times from 19 square kilometers to 260 square kilometers; under the condition of the concentration, the equivalent dispersion density D of the floating enteromorpha determined by the method of the inventionequ(0.12g L-1) The obtained Enteromorpha prolifera equivalent dispersion density in the middle of 6 months is 1.56g L by calculation as the initial biomass of the growth model simulation calculation-1The model calculation shows that the enteromorpha is increased by 13 times, and has no significant difference with the actual investigation result.
And (4) conclusion: the scientificity of the method is verified by evaluating the comparison between the calculation result of the existing biomass of the floating macroalgae and the field observation result and evaluating the comparison between the biomass simulation prediction result of the floating macroalgae growth model and the field observation result.
The method is applied to measuring and calculating the equivalent dispersion density of the enteromorpha and the apparent effective supply area (S) of the limiting factor based on the enteromorpha culture experimental data and combined with remote sensing image datawa) And limiting the depth of the effective feeding area of the factor, wherein the calculated existing biomass of the enteromorpha is basically consistent with the biomass of the enteromorpha which is observed and measured in the actual sea area. The increase multiple of the enteromorpha biomass calculated by the enteromorpha equivalent dispersion density has no significant difference with the increase multiple calculated by the actually measured enteromorpha coverage area. The result further verifies the reliability of the method, and simultaneously shows that the method has high application value and certain important value in reducing and preventing the disaster of the large-scale algal blooms in the actual sea area.
The embodiments are only for illustrating the technical idea of the present invention, and the technical idea of the present invention is not limited thereto, and any modifications made on the basis of the technical scheme according to the technical idea of the present invention fall within the scope of the present invention.
Claims (9)
1. A method for measuring and calculating the equivalent dispersion density of marine floating macroalgae is characterized by comprising the following steps:
(1) carrying out an experiment of the influence of the environmental restriction factor on the growth of the macroalgae;
(2) determining the limit effective supply volume of the environmental restriction factor and the theoretical limit equivalent dispersion density of the floating macroalgae based on the experimental result of the step (1);
(3) calculating an environmental restriction factor standard limit effective supply area and a standard limit effective supply radius;
(4) calculating the coverage area of the macroalgae plaque by using remote sensing image data and graphic analysis software, and calculating the apparent effective supply area of the environmental restriction factor by taking the standard limit effective supply radius calculated in the step (3) as a search distance;
(5) calculating the equivalent dispersion density of floating macroalgae under the condition of environmental restriction factor.
2. The method for measuring and calculating the equivalent dispersion density of marine floating macroalgae according to claim 1, wherein in the step (1), the offshore macroalgae is collected, the healthy algae are selected, the surface attachments are removed, the algae are repeatedly washed by filtered natural seawater for a plurality of times, the washed algae are temporarily stored in a refrigerator, then the culture experiment is carried out under the environmental condition controlled by an illumination incubator, the algae are taken out after an illumination period, the moisture on the surface of the algae is absorbed and then the wet weight is called and the volume is measured, and the level change of the limiting factor is measured after the culture solution is filtered.
3. The method for calculating the equivalent dispersion density of floating marine macroalgae according to claim 2, wherein the seawater is filtered through a 0.45 μm mixed fiber filter membrane, stored in a polyethylene tank, sterilized by a steam sterilizer at 120 ℃ for 15 minutes before the experiment, naturally cooled, and sufficiently shaken to recover the original amount of dissolved gas as a culture solution for the experiment.
4. The method for calculating the equivalent dispersion density of floating marine macroalgae according to claim 2, wherein during the cultivation experiment, the surface moisture of the algae is sucked dry, weighed, the algae with the same mass is placed in a plurality of containers, filtered, boiled and cooled culture solution with different volumes is placed in each container, nutritive salt is added to ensure that the restriction factor levels of the containers are the same, and then the containers are cultivated in an illumination incubator.
5. The method for measuring and calculating the equivalent dispersion density of floating marine macroalgae according to claim 2, wherein in the step (2), the Δ C of the level of the limiting factor before and after the illumination period under the condition of different volumes of the culture solution is calculated, the Δ C under the condition of different volumes of the culture solution forms a distribution, the volume corresponding to the Δ C when the percentile of the distribution is 5 is calculated as the limit effective supply volume V of the environmental limiting factorTDividing the cultivation mass m of the macroalgae by the limit effective supply volume VTObtaining the theoretical limit equivalent dispersion density D of the floating macroalgaeT。
6. The method for measuring and calculating the equivalent dispersion density of marine floating macroalgae according to claim 5, wherein in the step (3), the environmental restriction factor standard limit effective supply radius rTMCalculated as follows:
environmental restriction factor standard limit effective supply area STMCalculated as follows:
in the above formula, VTMIs the ultimate effective feed volume per mass of macroalgae.
7. The method for measuring and calculating the equivalent dispersion density of floating marine macroalgae according to claim 6, wherein in the step (4), the macroalgae plaque coverage area S is calculated by graphic analysis software analysis using the remote sensing image datacEffective supply radius scale r according to standard limitTMCalculating an apparent limiting effective supply radius R of macroalgae plaquesaWith RaAs the aggregation distance, scattered macroalgae patches are combined into a plurality of synthetic patches having a larger area by aggregation analysis, and the apparent effective supply region area S of each synthetic patch is calculatedwakS of each synthetic plaquewakSumming as an environmental limiting factor apparent effective supply area Swa。
8. The method for estimating equivalent dispersion density of marine floating macroalgae according to claim 7, wherein the apparent limiting effective feed radius RaCalculated as follows:
in the above formula, SrThe minimum resolution area of the remote sensing image is obtained.
9. The method for measuring and calculating the equivalent dispersion density of floating marine macroalgae according to claim 7, wherein in the step (5), the floating macroalgae is allowed to have the equivalent dispersion density Dequ＝DTObtaining the theoretical limit effective supply area S of the environmental restriction factorwT：
In the above formula, ρhtIs the own density of the macroalgae, rhowThe density of the culture broth itself, h1Statistical Length of Floating macroalgae, h2Depth of water h providing a limiting factor for floating macroalgae growth2＝h1+rTM；
When S iswa≤SwTThen:
when S iswa>SwTThen:
Dequ＝DT。
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| CN112465186A (en) * | 2020-10-28 | 2021-03-09 | 山东省科学院海洋仪器仪表研究所 | Method for predicting death days of sea surface floating enteromorpha prolifera |
| CN113177024A (en) * | 2021-06-29 | 2021-07-27 | 南京烽火星空通信发展有限公司 | Data global merging method under mass data scene |
| CN117333484A (en) * | 2023-11-30 | 2024-01-02 | 山东罗斯夫新材料科技有限公司 | Method for detecting acrylic emulsion production wastewater based on image processing |
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| CN113177024B (en) * | 2021-06-29 | 2021-09-14 | 南京烽火星空通信发展有限公司 | Data global merging method under mass data scene |
| CN117333484A (en) * | 2023-11-30 | 2024-01-02 | 山东罗斯夫新材料科技有限公司 | Method for detecting acrylic emulsion production wastewater based on image processing |
| CN117333484B (en) * | 2023-11-30 | 2024-02-20 | 山东罗斯夫新材料科技有限公司 | Method for detecting acrylic emulsion production wastewater based on image processing |
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