CN111671892A - Application of full-suspension duck embryo retinal cells in vaccine for egg drop syndrome of chickens - Google Patents
Application of full-suspension duck embryo retinal cells in vaccine for egg drop syndrome of chickens Download PDFInfo
- Publication number
- CN111671892A CN111671892A CN202010376819.3A CN202010376819A CN111671892A CN 111671892 A CN111671892 A CN 111671892A CN 202010376819 A CN202010376819 A CN 202010376819A CN 111671892 A CN111671892 A CN 111671892A
- Authority
- CN
- China
- Prior art keywords
- duck embryo
- retinal cells
- virus
- embryo retinal
- full
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000272525 Anas platyrhynchos Species 0.000 title claims abstract description 82
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 79
- 230000002207 retinal effect Effects 0.000 title claims abstract description 64
- 229960005486 vaccine Drugs 0.000 title claims abstract description 38
- 239000000725 suspension Substances 0.000 title claims abstract description 35
- 208000011580 syndromic disease Diseases 0.000 title claims abstract description 24
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 18
- 235000013330 chicken meat Nutrition 0.000 title claims abstract description 18
- 241000700605 Viruses Species 0.000 claims abstract description 71
- 241000886677 Duck adenovirus 1 Species 0.000 claims abstract description 19
- 239000000427 antigen Substances 0.000 claims abstract description 12
- 102000036639 antigens Human genes 0.000 claims abstract description 12
- 108091007433 antigens Proteins 0.000 claims abstract description 12
- 239000012646 vaccine adjuvant Substances 0.000 claims abstract description 4
- 229940124931 vaccine adjuvant Drugs 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 claims description 23
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 16
- 238000011081 inoculation Methods 0.000 claims description 13
- 239000002609 medium Substances 0.000 claims description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 238000010257 thawing Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000010261 cell growth Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 244000144977 poultry Species 0.000 abstract description 3
- 235000013594 poultry meat Nutrition 0.000 abstract description 3
- 210000000991 chicken egg Anatomy 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 description 24
- 238000004519 manufacturing process Methods 0.000 description 6
- 210000002257 embryonic structure Anatomy 0.000 description 5
- 230000005847 immunogenicity Effects 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000008014 freezing Effects 0.000 description 4
- 238000007710 freezing Methods 0.000 description 4
- 229940031551 inactivated vaccine Drugs 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 210000001525 retina Anatomy 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 101710112752 Cytotoxin Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 231100000433 cytotoxic Toxicity 0.000 description 2
- 231100000599 cytotoxic agent Toxicity 0.000 description 2
- 230000001472 cytotoxic effect Effects 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 239000002619 cytotoxin Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
- 241000238367 Mya arenaria Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 230000001779 embryotoxic effect Effects 0.000 description 1
- 231100000238 embryotoxicity Toxicity 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000013587 production medium Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229940031626 subunit vaccine Drugs 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10211—Aviadenovirus, e.g. fowl adenovirus A
- C12N2710/10234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Virology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Communicable Diseases (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010376819.3A CN111671892A (en) | 2020-05-07 | 2020-05-07 | Application of full-suspension duck embryo retinal cells in vaccine for egg drop syndrome of chickens |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010376819.3A CN111671892A (en) | 2020-05-07 | 2020-05-07 | Application of full-suspension duck embryo retinal cells in vaccine for egg drop syndrome of chickens |
Publications (1)
Publication Number | Publication Date |
---|---|
CN111671892A true CN111671892A (en) | 2020-09-18 |
Family
ID=72451754
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010376819.3A Pending CN111671892A (en) | 2020-05-07 | 2020-05-07 | Application of full-suspension duck embryo retinal cells in vaccine for egg drop syndrome of chickens |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111671892A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112359142A (en) * | 2020-11-10 | 2021-02-12 | 成都天邦生物制品有限公司 | Method for determining newcastle disease virus content by using whole suspension cells |
CN113122508A (en) * | 2021-03-30 | 2021-07-16 | 成都天邦生物制品有限公司 | Infectious bronchitis cell adaptive virus and application thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110229969A1 (en) * | 2008-06-25 | 2011-09-22 | Volker Sandig | Cell Line for Propagation of Highly Attenuated AlphaViruses |
CN108721615A (en) * | 2018-05-16 | 2018-11-02 | 北京市农林科学院 | A kind of method and its vaccine preparing duck tembusu virus inactivated vaccine |
CN109593729A (en) * | 2018-12-29 | 2019-04-09 | 肇庆大华农生物药品有限公司 | A kind of fowl Egg Drop syndrome virus cultural method and its application in vaccine |
CN110129281A (en) * | 2019-05-15 | 2019-08-16 | 成都天邦生物制品有限公司 | A kind of method and application using suspension cell culture newcastle disease virus |
-
2020
- 2020-05-07 CN CN202010376819.3A patent/CN111671892A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110229969A1 (en) * | 2008-06-25 | 2011-09-22 | Volker Sandig | Cell Line for Propagation of Highly Attenuated AlphaViruses |
CN108721615A (en) * | 2018-05-16 | 2018-11-02 | 北京市农林科学院 | A kind of method and its vaccine preparing duck tembusu virus inactivated vaccine |
CN109593729A (en) * | 2018-12-29 | 2019-04-09 | 肇庆大华农生物药品有限公司 | A kind of fowl Egg Drop syndrome virus cultural method and its application in vaccine |
CN110129281A (en) * | 2019-05-15 | 2019-08-16 | 成都天邦生物制品有限公司 | A kind of method and application using suspension cell culture newcastle disease virus |
Non-Patent Citations (1)
Title |
---|
INGO JORDAN等: "Continuous cell lines from the Muscovy duck as potential replacement for primary cells in the production of avian vaccines" * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112359142A (en) * | 2020-11-10 | 2021-02-12 | 成都天邦生物制品有限公司 | Method for determining newcastle disease virus content by using whole suspension cells |
CN113122508A (en) * | 2021-03-30 | 2021-07-16 | 成都天邦生物制品有限公司 | Infectious bronchitis cell adaptive virus and application thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107267466B (en) | Method for large-scale production of porcine pseudorabies inactivated vaccine | |
CN107904215B (en) | Full suspension culture method of avian influenza virus | |
CN108300704B (en) | Method for suspension culture of infectious bronchitis virus by using continuous cell line | |
CN111632137A (en) | Triple vaccine for feline calicivirus disease, feline infectious rhinotracheitis and feline panleukopenia as well as preparation method and application thereof | |
CN110237246B (en) | Method for culturing inactivated vaccine for avian influenza (H9) by using full-suspension cells | |
CN111671892A (en) | Application of full-suspension duck embryo retinal cells in vaccine for egg drop syndrome of chickens | |
CN109913404B (en) | Preparation method of chicken infectious bursal disease virus live vaccine | |
CN102268411A (en) | Method for IBDV (Infectious Bursal Disease Virus) serum-free microcarrier suspension culture proliferation | |
CN110157659B (en) | BHK-21 cell line BHK-21-BP-2 clone strain and application thereof in full suspension cell culture | |
CN113122508A (en) | Infectious bronchitis cell adaptive virus and application thereof | |
CN114276981A (en) | Vero-E6 suspension cell strain sVero-E6 adapted to porcine epidemic diarrhea virus and application thereof | |
CN106237323B (en) | Porcine reproductive and respiratory syndrome purified vaccine and preparation method thereof | |
CN109593729A (en) | A kind of fowl Egg Drop syndrome virus cultural method and its application in vaccine | |
CN115627259B (en) | Adaptation method of virus in chick embryo fibroblast | |
CN111569060A (en) | Preparation method of egg drop syndrome vaccine and vaccine | |
CN113817688B (en) | Chick embryo culture process of tetravalent influenza virus split vaccine and preparation method of tetravalent influenza virus split vaccine | |
CN114395536B (en) | Avian adenovirus type 4, 8 and 11 trivalent vaccine and preparation method and application thereof | |
CN112143714B (en) | Method for producing H7 subtype avian influenza virus inactivated vaccine by using low-immunity chick embryo | |
CN111411087B (en) | Cyprinus carpioides herpesvirus II type low virulent strain and application thereof | |
CN104740627B (en) | A kind of large-scale method for producing of pseudo- mad dog attenuated live vaccines for animals | |
CN102978166A (en) | Method for preparing newcastle disease viruses and vaccines by using serial passage cells, and products thereof | |
CN103157107A (en) | Chicken Marek's disease vaccine produced by using continuous passage cell line and production method | |
CN107261132B (en) | Method for producing porcine pseudorabies live vaccine by using bioreactor and product thereof | |
CN107198771B (en) | Method for producing porcine pseudorabies gE gene deletion virus vaccine by microcarrier suspension culture cells | |
CN112080478A (en) | Efficient propagation method and application of H5 subtype avian influenza virus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20221215 Address after: No.358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan 610000 Applicant after: CHENGDU TECBOND BIOLOGICAL PRODUCTS CO.,LTD. Address before: No.358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan 610000 Applicant before: CHENGDU TECBOND BIOLOGICAL PRODUCTS CO.,LTD. Applicant before: Shiji Biotechnology (Nanjing) Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20230307 Address after: No.358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan 610000 Applicant after: Chengdu Shiji biopharmaceutical Co.,Ltd. Address before: No.358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan 610000 Applicant before: CHENGDU TECBOND BIOLOGICAL PRODUCTS CO.,LTD. Applicant before: Shiji Biotechnology (Nanjing) Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200918 |
|
RJ01 | Rejection of invention patent application after publication |