CN111671667A

CN111671667A - Active collagen freeze-dried powder and preparation method thereof

Info

Publication number: CN111671667A
Application number: CN202010615460.0A
Authority: CN
Inventors: 唐小江; 张晓丽; 胡伟; 肖凌琥; 梁炤垣
Original assignee: Guangdong Zoolgen Bio Tec Co ltd
Current assignee: Guangdong Zoolgen Bio Tec Co ltd
Priority date: 2020-06-30
Filing date: 2020-06-30
Publication date: 2020-09-18
Anticipated expiration: 2040-06-30
Also published as: CN111671667B

Abstract

The invention discloses active collagen freeze-dried powder and a preparation method thereof. According to the invention, the rat tail collagen, the polyalcohol, the trehalose and the citric acid are compounded to improve the stability of the rat tail collagen in the freeze drying process, and the prepared freeze-dried product can be stored for a long time without adding a preservative, has good re-solubility, can keep the natural biological activity of the collagen, and has the capability of remarkably promoting the synthesis of skin collagen.

Description

Active collagen freeze-dried powder and preparation method thereof

Technical Field

The invention relates to the technical field of collagen, in particular to active collagen freeze-dried powder and a preparation method thereof.

Background

Collagen is widely present in various vertebrate bodies, belongs to structural proteins, and can provide mechanical strength to connective tissues such as tendons, bones, cartilage, teeth, skin, blood vessels, and the like. The collagen includes various types from type I to type XII, wherein type I collagen is most common.

The I type collagen consists of two identical alpha 1 (I) peptide chains and a different alpha 2 (I) peptide chain, and the three peptide chains are wound into a special three-strand helical structure and can be used as a bracket for cell attachment and migration; and arginine-glycine-aspartic acid (RGD) in the peptide chain is a binding site of cell adhesion molecules (such as integrin), and can effectively promote proliferation and differentiation of cells.

Based on the functions and characteristics of collagen, collagen can be used as a good skin care material. However, the preservation condition of the macromolecular collagen is high, the macromolecular collagen can be inactivated when the temperature is higher than 45 ℃, the triple-helix structure is dispersed, the collagen is rich in nutrients and easy to nourish bacteria, a common skin care product generally has a preservation period of 6-18 months after being uncapped, a large amount of preservative is generally required to be added, but the preservative has obvious irritation to the skin, and the skin is sensitive, elastic and glossy after long-term use. Therefore, it is important to develop a method for preserving collagen in which the amount of the preservative added is reduced or even no preservative is used.

Freeze-drying is a common method for storing protein by adopting a vacuum freeze-drying technology and dehydrating materials by utilizing a sublimation principle. The freeze-dried product is usually small in packaging specification, is dissolved at present when being used, and does not need to be added with a preservative; the freeze-dried collagen can still maintain a triple-helix structure and has the functions of promoting cell proliferation and differentiation. However, the existing freeze-dried collagen still has the following defects: 1) the collagen has special amino acid content and proportion, so that the freeze-dried collagen is difficult to re-dissolve, and the dissolving concentration of the common freeze-dried collagen is not higher than 0.2%; 2) in order to increase the dissolving capacity of natural active collagen, partial groups in the natural collagen are usually modified through acylation, esterification and the like, so that the stability of the collagen is reduced, arginine in arginine-glycine-aspartic acid in the active collagen changes the structure, the activity of the collagen for promoting cell growth is reduced, and the efficacy of the collagen is further reduced or lost; 3) the raw materials of some freeze-dried products adopt recombinant collagen or hydrolyzed and enzymolyzed collagen peptide, and the collagen peptide does not have the bioactivity of collagen.

Disclosure of Invention

The active collagen freeze-dried powder can be stored for a long time without adding a preservative, can keep the natural activity of collagen, and can be quickly redissolved under a neutral condition.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

an active collagen freeze-dried powder comprises collagen, polyalcohol, trehalose and citric acid, and the collagen is rat tail collagen.

Compared with other types of collagen, the rat tail collagen selected by the invention has higher solubility, and the ice crystals in the collagen grow gradually to damage the structure of the collagen in the freeze-drying process, so that a freeze-drying protective agent needs to be added before freeze-drying, a good skeleton is endowed to the collagen in the freeze-drying process, and the damage to the collagen caused by freeze dehydration is reduced. The active ingredients are different, and the types of suitable freeze-drying protective agents and redissolving auxiliary agents are different, the rat tail collagen is used as the active ingredient, the polyol is used as the excipient, so that the viscosity can be increased, the crystallization rate of water can be reduced, the collagen is stable, the surface atrophy and collapse of freeze-dried products can be avoided, the trehalose can keep water molecules in the collagen in a low-temperature environment, the three-dimensional spiral structure of the collagen can be maintained, and the citric acid can promote the dissolution of the collagen. According to the invention, the rat tail collagen, the polyalcohol, the trehalose and the citric acid are compounded to improve the stability of the rat tail collagen in the freeze drying process, and the prepared freeze-dried product can keep the natural biological activity of the collagen, has better re-solubility compared with acylated collagen or other types of non-acylated collagen, and can be quickly re-dissolved under a neutral condition.

The variety and the proportion of the freeze-drying protective agent have obvious influence on the protective effect of the rat tail collagen, the mass ratio of the collagen, the polyalcohol, the trehalose and the citric acid is preferably 1:10-25:5-10:0.25-0.5, more preferably 1:20-25:5-7.5:0.25-0.5, and the solubility and the stability of a rat tail collagen freeze-dried product are improved.

Preferably, the polyhydric alcohol is mannitol, and has a good protective effect on rat tail collagen in a freeze drying process.

Preferably, the rat tail collagen is rat tail tendon type I collagen. The rat tail collagen of the invention is collagen extracted from the tail tendon of a rat, the tail tendon of the rat has bundled collagen, and is basically type I collagen, especially for the old rat, the tail of the rat is bigger, the collagen content is high, and compared with other types of collagen, the type I collagen of the tail tendon of the rat has higher solubility.

The invention also provides a preparation method of the active collagen freeze-dried powder, which comprises the following steps:

(1) adding mannitol, trehalose and citric acid into the collagen solution, and stirring until the mannitol, the trehalose and the citric acid are completely dissolved;

(2) and (2) freeze-drying the mixed solution obtained in the step (1) to obtain the active collagen freeze-dried powder.

The active collagen freeze-dried powder prepared by the method has excellent stability and re-solubility, rapid dissolution, good formability and uniform texture.

In addition, the inventor tests and finds that the addition sequence of mannitol, trehalose and citric acid in the preparation method has little influence on the stability and re-solubility of the finally prepared active collagen freeze-dried powder.

Preferably, in the step (1), the concentration of the collagen in the collagen solution is 4g/L, which is more beneficial to controlling the content of the collagen in the freeze-dried product.

Preferably, in the step (1), the preparation method of the collagen comprises: collagen in rat tail tendons, especially SPF SD rat tail tendons, is extracted by an acid-enzyme mixing method, and freeze-drying is carried out to obtain rat tail collagen freeze-dried powder which is free of pathogens and high in cleanliness.

The freeze-drying powder of the invention can be prepared by adopting a freeze-drying method well known to those skilled in the art, and the freeze-drying curve is further optimized by the invention, and in the step (2), the freeze-drying procedure is as follows:

temperature of	Setting time	Retention time
			-70～-60℃	0min	45～60min
20～30℃	400～600min	240～420min
			-15～-5℃	240～480min	240～480min
0～10℃	180～300min	480～600min

A more preferred freeze-drying procedure is as follows:

temperature of	Setting time	Retention time
			-70℃	0min	60min
25℃	480min	360min
			-10℃	360min	360min
5℃	240min	560min

。

The collagen freeze-dried product prepared by the optimized freeze-drying method has better morphological appearance and redissolution effect.

Preferably, the pH of the mixed solution in the step (2) is 6.0 to 7.0. The pH values of the active collagen freeze-dried powder before freeze-drying and after redissolution are basically stable, are neutral and do not irritate the skin.

The active collagen freeze-dried powder can be redissolved by diluent or purified water, and the redissolution is generally carried out at the temperature of 25 ℃.

The invention also provides application of the active collagen freeze-dried powder or the active collagen freeze-dried powder prepared by the method in cosmetics. The active collagen freeze-dried powder can be stored for a long time without adding a preservative, can keep the natural biological activity of collagen, can effectively promote self cells to generate collagen, and has good application prospect in cosmetics.

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the invention, the rat tail collagen, the polyalcohol, the trehalose and the citric acid are compounded to jointly improve the stability of the rat tail collagen in the freeze drying process, and the prepared active collagen freeze-dried powder can keep the natural activity of the collagen;

(2) the active collagen freeze-dried powder can be quickly redissolved under a neutral condition, and the dissolving concentration is higher;

(3) the active collagen freeze-dried powder can be stored for a long time under the condition of not adding a preservative;

(4) the active collagen freeze-dried powder has the capability of remarkably promoting the synthesis of skin collagen and has good application prospect in cosmetics.

Drawings

FIG. 1 is a graph showing the effect of different collagen lyophilized powders on the expression level of COL1A1 mRNA.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples. It will be understood by those skilled in the art that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In the examples, the experimental methods used were all conventional methods unless otherwise specified, and the materials, reagents and the like used were commercially available without otherwise specified.

Example 1

The preparation method of the active collagen freeze-dried powder comprises the following steps:

1) extracting collagen in SPF-level SD rat tail tendon by acid-enzyme mixing method;

2) respectively taking 3 parts of 100mL of collagen extracted in the step 1), freeze-drying, drying at 37 ℃ for 24 hours, weighing the weight m (g) of the freeze-dried collagen, and calculating the average value

The concentration of the collagen of the batch is obtained;

3) adjusting the concentration of the collagen obtained in the step 2) to 4g/L by using sterilized purified water;

4) adding 4g of mannitol into 100mL of 4g/L collagen, and uniformly stirring until the mannitol is completely dissolved;

5) adding 2g of trehalose into the mixed solution obtained in the step 4), and uniformly stirring until the trehalose is completely dissolved;

6) adding 0.1g of citric acid into the mixed solution obtained in the step 5), and uniformly stirring until the citric acid is completely dissolved;

7) subpackaging the mixed solution obtained in the step 6) into sterilized penicillin bottles according to the specification of 2 mL/bottle, and freeze-drying to obtain the active collagen freeze-dried powder, wherein the freeze-drying procedure is shown in table 1:

TABLE 1

Example 2

The concentration of the collagen of the batch is obtained;

4) adding 6g of mannitol into 100mL of 4g/L collagen, and uniformly stirring until the mannitol is completely dissolved;

7) and (3) subpackaging the mixed solution obtained in the step 6) into sterilized penicillin bottles according to the specification of 2 mL/bottle, and freeze-drying the penicillin bottles, wherein the procedures of freeze-drying are the same as those in the example 1, so as to obtain the active collagen freeze-dried powder.

Example 3

The concentration of the collagen of the batch is obtained;

4) adding 10g of mannitol into 100mL of 4g/L collagen, and uniformly stirring until the mannitol is completely dissolved;

Example 4

The concentration of the collagen of the batch is obtained;

4) adding 8g of mannitol into 100mL of 4g/L collagen, and uniformly stirring until the mannitol is completely dissolved;

5) adding 3g of trehalose into the mixed solution obtained in the step 4), and uniformly stirring until the trehalose is completely dissolved.

6) Adding 0.2g of citric acid into the mixed solution obtained in the step 5), and uniformly stirring until the citric acid is completely dissolved;

Example 5

The concentration of the collagen of the batch is obtained;

5) adding 4g of trehalose into the mixed solution obtained in the step 4), and uniformly stirring until the trehalose is completely dissolved;

Example 6

The concentration of the collagen of the batch is obtained;

Comparative example 1

The concentration of the collagen of the batch is obtained;

5) adding 4g of trehalose into the mixed solution obtained in the step 4), and uniformly stirring until the trehalose is completely dissolved.

6) And (3) subpackaging the mixed solution obtained in the step 5) into sterilized penicillin bottles according to the specification of 2 mL/bottle, and freeze-drying the penicillin bottles, wherein the procedures of freeze-drying are the same as those in the example 1, so as to obtain the active collagen freeze-dried powder.

Comparative example 2

The concentration of the collagen of the batch is obtained;

4) adding 2g of mannitol into 100mL of 4g/L collagen, and uniformly stirring until the mannitol is completely dissolved;

5) adding 6g of trehalose into the mixed solution obtained in the step 4), and uniformly stirring until the trehalose is completely dissolved.

Comparative example 3

The preparation method of the acylated collagen freeze-dried powder comprises the following steps:

1) extracting collagen in the pigskin by adopting an acid-enzyme mixing method;

2) placing the collagen extracted in the step 1) on a magnetic stirrer, and slowly dropwise adding lauroyl chloride while stirring, wherein the adding ratio of the collagen to the lauroyl chloride is 1 g: 0.12mL, using NaOH solution to control the pH value of a reaction system to be 8-9 in the reaction process, after reacting for a period of time, using ethanol to precipitate a product, and washing to obtain acylated collagen;

3) respectively taking 3 parts of 100mL of acylated collagen obtained in the step 3), freeze-drying, drying at 37 ℃ for 24 hours, weighing m (g) of the weight of the freeze-dried collagen, and calculating the average value

The concentration of the collagen of the batch is obtained;

4) adjusting the concentration of the collagen obtained in the step 3) to 4g/L by using sterilized purified water;

5) adding 8g of mannitol into 100mL of 4g/L collagen, and uniformly stirring until the mannitol is completely dissolved;

6) adding 4g of trehalose into the mixed solution obtained in the step 5), and uniformly stirring until the trehalose is completely dissolved;

7) adding 0.2g of citric acid into the mixed solution obtained in the step 6), and uniformly stirring until the citric acid is completely dissolved;

8) and (3) subpackaging the mixed solution obtained in the step 7) into sterilized penicillin bottles according to the specification of 2 mL/bottle, and freeze-drying the penicillin bottles, wherein the procedures of freeze-drying are the same as those in the example 1, so as to obtain the active collagen freeze-dried powder.

Comparative example 4

1) extracting collagen in the pigskin by adopting an acid-enzyme mixing method;

The concentration of the collagen of the batch is obtained;

(I) testing of reconstitution Properties

The lyophilized powders of the above examples and comparative examples were taken and stored in 3 bottles per group, 100mL of sterilized purified water was added to each bottle, the reconstitution time was observed by gentle shaking, and the collagen concentration of each group after the solubilization was measured, and the results are shown in Table 2.

TABLE 2

As can be seen from the results in Table 2, the lyophilized active collagen powders of examples 1 to 6 have shorter reconstitution time, higher solubility and better reconstitution property than those of comparative examples 1 to 4. In addition, the pH of the mixed solution before lyophilization in step 7) of examples 1 to 6 was 6.0 to 7.0, and the pH after reconstitution was substantially stable without irritating the skin. Therefore, the collagen freeze-dried powder with excellent redissolution performance is prepared by preferably selecting the type of the collagen and the proportion of the collagen, the polyalcohol, the trehalose and the citric acid.

(II) COL1A1 mRNA expression level

HaCaT cells (human immortalized epidermal cells) were prepared to a cell concentration of 5 × 10⁵one/mL of the suspension and plated on 6-well plates; put in CO₂Incubator (5% CO)₂37 ℃) for 24 hours, and then the test samples of example 6 and comparative example 3 were added, respectively, toThe control group is conventional cell culture, and no acylated collagen and the collagen sample of the invention are added; the culture was continued for 24 hours. After 24 hours, the culture solution is firstly pumped away, then the RNA of the cells is extracted by using an RNAzol RNA extraction reagent, and then the concentration of the RNA is measured by using a Nanodrop; then, RNA was reverse-transcribed into cDNA using oligo (dT), dNTP, 5X first strand buffer, recombinant ribonuclease inhibitor (RNaseOUT inhibitor), Dithiothreitol (DTT) and murine leukemia reverse transcriptase (M-MLV) to perform Real-time polymerase chain Reaction (rt-PCR).

The technique of quantifying the amount of a target gene (COLIA1 gene) in a sample by detection with a fluorescent dye allows one to detect multiple reactions at the same time using well plates of different volumes. In each reaction, a cDNA sample, an upstream primer, a downstream primer and a SybrGreen fluorescent dye were added, and the primer sequences were as follows: COLIA 1F-primer 5'-TCTGCG ACA ACGGCA AGG TG-3', COL1A 1R-primer 5'-GAC GCC GGT GGT TTC TTG GT-3', and reuse

480 the results of detecting the cell gene expression changes under different drugs are shown in FIG. 1.

The results of figure 1 show that the invention can maintain the natural biological activity of collagen, and the prepared active collagen freeze-dried powder has the capability of remarkably promoting the synthesis of skin collagen, and the activity of the active collagen freeze-dried powder is obviously superior to that of acylated collagen.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The active collagen freeze-dried powder is characterized by comprising collagen, polyhydric alcohol, trehalose and citric acid, wherein the collagen is rat tail collagen.

2. The active collagen freeze-dried powder according to claim 1, wherein the mass ratio of the collagen, the polyalcohol, the trehalose and the citric acid is 1:10-25:5-10: 0.25-0.5.

3. The active collagen freeze-dried powder according to claim 2, wherein the mass ratio of the collagen, the polyalcohol, the trehalose and the citric acid is 1:20-25:5-7.5: 0.25-0.5.

4. Active collagen freeze-dried powder according to claim 1, characterized in that the polyol is mannitol.

5. A lyophilized active collagen powder according to any one of claims 1 to 4, wherein said rat tail collagen is rat tail tendon type I collagen.

6. A method for preparing active collagen freeze-dried powder according to any one of claims 1 to 5, which comprises the following steps:

7. The method for preparing lyophilized powder of active collagen according to claim 6, wherein in the step (1), the method for preparing collagen comprises: extracting collagen in rat tail tendon by acid-enzyme mixing method, and freeze drying to obtain lyophilized powder of rat tail collagen.

8. The method for preparing lyophilized powder of active collagen according to claim 6, wherein in the step (2), the procedure of lyophilization is as follows:

。

9. The method for preparing lyophilized powder of active collagen according to claim 6, wherein the pH of the mixed solution in step (2) is 6.0-7.0.

10. Use of the lyophilized active collagen powder according to any one of claims 1 to 5 or prepared according to the method of any one of claims 6 to 9 in cosmetics.