CN111670950A - Preparation method of fermented dairy product with immunoregulation function - Google Patents

Preparation method of fermented dairy product with immunoregulation function Download PDF

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Publication number
CN111670950A
CN111670950A CN202010649811.XA CN202010649811A CN111670950A CN 111670950 A CN111670950 A CN 111670950A CN 202010649811 A CN202010649811 A CN 202010649811A CN 111670950 A CN111670950 A CN 111670950A
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CN
China
Prior art keywords
mixing
dairy product
stirring
fermented dairy
fermented
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Pending
Application number
CN202010649811.XA
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Chinese (zh)
Inventor
李建国
赵瑞生
梁茂文
王志俊
王呈
马馨
段亚娜
穆秀梅
刘静
冯继永
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Shanxi Agricultural University
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Shanxi Agricultural University
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Priority to CN202010649811.XA priority Critical patent/CN111670950A/en
Publication of CN111670950A publication Critical patent/CN111670950A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/517Bifidum

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a preparation method of a fermented dairy product with an immunoregulation function, which comprises the following steps: taking 50-70g of schisandra chinensis, 50-70g of mulberry and 80-100g of nostoc sphaeroides, crushing, sieving with a 10-mesh sieve, adding tap water, adjusting the pH to 6.0-7.0, adding alpha-amylase, hydrolyzing, adjusting the pH to 7.0-8.0, adding compound protease, stirring for enzymolysis for 3 hours, debitterizing and decoloring, filtering with a plate frame, desalting and concentrating by a nanofiltration membrane, adding 20-30 g of inulin and 0.05-0.08 g of citric acid, stirring uniformly, mixing with sterilized milk in equal volume, placing in a fermentation tank, adding zymocyte powder, sealing and fermenting at 26-30 ℃ to obtain the oral liquid. The fermented milk product obtained by the invention has good taste, and has the health-care effects of resisting virus, bidirectionally regulating blood sugar, improving the immunity of the organism, preventing and treating cardiovascular and cerebrovascular diseases, improving the intestinal function and promoting the absorption of calcium.

Description

Preparation method of fermented dairy product with immunoregulation function
Technical Field
The invention relates to the field of health-care product preparation, in particular to a preparation method of a fermented dairy product with an immunoregulation function.
Background
The functional beverage is a soft beverage which is added with certain functional factors and has the health-care functions of regulating the body function, enhancing the immunity and the like while quenching thirst. The functional beverage is a new growth point of food industry at home and abroad at present. In view of the attention of consumers to food safety in recent years, the extraction of functional food additives and ingredients from natural products, particularly from commonly consumed plants, has become a hot spot for development at home and abroad.
Disclosure of Invention
The invention aims to provide a preparation method of a fermented dairy product with an immunoregulation function, and the fermented dairy product has good taste, and has health care effects of resisting viruses, bidirectionally regulating blood sugar, improving immunity of organisms, preventing and treating cardiovascular and cerebrovascular diseases, improving intestinal functions and promoting calcium absorption.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for preparing a fermented dairy product with immunoregulation function comprises the following steps:
s1, taking 50-70g of schisandra chinensis, 50-70g of mulberry and 80-100g of nostoc sphaeroids kutz, crushing, sieving by a 10-mesh sieve, adding tap water according to the material-liquid ratio of 1:5, adjusting the pH to 6.0-7.0, adding 3 per thousand (v/v) of alpha-amylase, hydrolyzing at 90 ℃ for 2 hours, removing starch in an extracting solution, adjusting the pH to 7.0-8.0, adding 7 per thousand (v/v) of compound protease, stirring at 35 ℃ for enzymolysis for 3 hours, adding a mixture of activated carbon and diatomite with 3 percent of liquid amount, stirring and adsorbing at 80-90 ℃ for 15-25 minutes, debitterizing and decolorizing, filtering by a plate frame, desalting by a nanofiltration membrane and concentrating to obtain a concentrated solution;
s2, adding 20-30 g of inulin and 0.05-0.08 g of citric acid into the obtained concentrated solution, uniformly stirring, mixing with sterilized milk in equal volume, placing into a fermentation tank, adding zymophyte powder in proportion, sealing and storing at 26-30 ℃, and fermenting for 3-7 days to obtain the product.
Further, the zymocyte powder is obtained by mixing bifidobacterium bifidum and lactobacillus plantarum according to the mass ratio of 1: 3.
Further, in the step S2, the addition amount of the zymophyte powder is 5-10%.
Further, the compound protease is prepared by mixing pepsin and papain in a mass ratio of 1: 1 mixing the components.
The invention has the following beneficial effects:
the obtained fermented milk product has good taste, and has health promotion effects of resisting virus, regulating blood sugar bidirectionally, enhancing immunity, preventing and treating cardiovascular disease and cerebrovascular disease, improving intestinal function, and promoting calcium absorption.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the following examples, the zymocyte powder is obtained by mixing bifidobacterium bifidum and lactobacillus plantarum according to the mass ratio of 1: 3. The compound protease is prepared from pepsin and papain in a mass ratio of 1: 1 mixing the components.
Example 1
A method for preparing a fermented dairy product with immunoregulation function comprises the following steps:
s1, taking 50 g of schisandra chinensis, 50 g of mulberry and 80 g of nostoc sphaeroids kutz, crushing, sieving with a 10-mesh sieve, adding tap water according to the material-liquid ratio of 1:5, adjusting the pH to 6.0-7.0, adding 3 per thousand (v/v) of alpha-amylase, hydrolyzing at 90 ℃ for 2 hours, removing starch in an extracting solution, adjusting the pH to 7.0-8.0, adding 7 per thousand (v/v) of compound protease, stirring at 35 ℃ for enzymolysis for 3 hours, adding a mixture of activated carbon and kieselguhr with the liquid amount of 3%, stirring at 80-90 ℃ for adsorption for 15-25 minutes, debitterizing, decolorizing, filtering with a plate frame, desalting with a nanofiltration membrane, and concentrating to obtain a concentrated solution;
s2, adding 20g of inulin and 0.05g of citric acid into the obtained concentrated solution, uniformly stirring, mixing with sterilized milk in equal volume, placing into a fermentation tank, adding zymophyte powder according to the proportion of 5-10% (v/v), sealing and storing at 26-30 ℃, and fermenting for 3-7 days to obtain the product.
Example 2
A method for preparing a fermented dairy product with immunoregulation function comprises the following steps:
s1, taking 70g of schisandra chinensis, 70g of mulberry and 100g of nostoc sphaeroids kutz, crushing, sieving with a 10-mesh sieve, adding tap water according to the material-liquid ratio of 1:5, adjusting the pH to 6.0-7.0, adding 3 per thousand (v/v) of alpha-amylase, hydrolyzing at 90 ℃ for 2h, removing starch in an extracting solution, adjusting the pH to 7.0-8.0, adding 7 per thousand (v/v) of compound protease, stirring at 35 ℃ for enzymolysis for 3h, adding a mixture of activated carbon and kieselguhr with the liquid amount of 3%, stirring at 80-90 ℃ for adsorption for 15-25min, debitterizing, decolorizing, filtering with a plate frame, desalting with a nanofiltration membrane, and concentrating to obtain a concentrated solution;
s2, adding 30 g of inulin and 0.08 g of citric acid into the obtained concentrated solution, uniformly stirring, mixing with sterilized milk in equal volume, placing into a fermentation tank, adding zymophyte powder according to the proportion of 5-10% (v/v), sealing and storing at 26-30 ℃, and fermenting for 3-7 days to obtain the product.
Example 3
A method for preparing a fermented dairy product with immunoregulation function comprises the following steps:
s1, taking 60g of schisandra chinensis, 60g of mulberry and 90g of nostoc sphaeroids kutz, crushing, sieving with a 10-mesh sieve, adding tap water according to the material-liquid ratio of 1:5, adjusting the pH to 6.0-7.0, adding 3 per thousand (v/v) of alpha-amylase, hydrolyzing at 90 ℃ for 2 hours, removing starch in an extracting solution, adjusting the pH to 7.0-8.0, adding 7 per thousand (v/v) of compound protease, stirring at 35 ℃ for enzymolysis for 3 hours, adding a mixture of activated carbon and kieselguhr with the liquid amount of 3%, stirring and adsorbing at 80-90 ℃ for 15-25 minutes, debitterizing, decoloring, filtering with a plate frame, desalting with a nanofiltration membrane, and concentrating to obtain a concentrated solution;
s2, adding 25g of inulin and 0.065 g of citric acid into the obtained concentrated solution, uniformly stirring, mixing with sterilized milk in equal volume, placing into a fermentation tank, adding zymophyte powder according to the proportion of 5-10% (v/v), sealing and storing at 26-30 ℃, and fermenting for 3-7 days to obtain the product.
The invention has the advantages of palatable sweetness, delicate and smooth taste and rich nutrition, has the health-care efficacies of resisting virus, bidirectionally regulating blood sugar, improving the immunity of the organism, preventing and treating cardiovascular and cerebrovascular diseases, improving the intestinal function and promoting the absorption of calcium, and has no pathogenic bacteria detected.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.

Claims (4)

1. A preparation method of a fermented dairy product with an immunoregulation function is characterized by comprising the following steps:
s1, taking 50-70g of schisandra chinensis, 50-70g of mulberry and 80-100g of nostoc sphaeroids kutz, crushing, sieving by a 10-mesh sieve, adding tap water according to the material-liquid ratio of 1:5, adjusting the pH to 6.0-7.0, adding 3 per thousand (v/v) of alpha-amylase, hydrolyzing at 90 ℃ for 2 hours, removing starch in an extracting solution, adjusting the pH to 7.0-8.0, adding 7 per thousand (v/v) of compound protease, stirring at 35 ℃ for enzymolysis for 3 hours, adding a mixture of activated carbon and diatomite with 3 percent of liquid amount, stirring and adsorbing at 80-90 ℃ for 15-25 minutes, debitterizing and decolorizing, filtering by a plate frame, desalting by a nanofiltration membrane and concentrating to obtain a concentrated solution;
s2, adding 20-30 g of inulin and 0.05-0.08 g of citric acid into the obtained concentrated solution, uniformly stirring, mixing with sterilized milk in equal volume, placing into a fermentation tank, adding zymophyte powder in proportion, sealing and storing at 26-30 ℃, and fermenting for 3-7 days to obtain the product.
2. The method for preparing a fermented dairy product with immunoregulatory function according to claim 1, wherein the fermented bacterial powder is obtained by mixing bifidobacterium bifidum and lactobacillus plantarum in a mass ratio of 1: 3.
3. The method of claim 1, wherein in step S2, the amount of the fermented powder is 5-10%.
4. The method for preparing the fermented dairy product with the immunoregulation function according to claim 1, wherein the compound protease is prepared by mixing pepsin and papain in a mass ratio of 1: 1 mixing the components.
CN202010649811.XA 2020-07-08 2020-07-08 Preparation method of fermented dairy product with immunoregulation function Pending CN111670950A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2169472C2 (en) * 2000-06-19 2001-06-27 Цинберг Марк Беньяминович Method of preparing bacterial ferment for fermented-milk product
CN105815777A (en) * 2016-03-29 2016-08-03 陕西富士金医药保健品有限责任公司 Preparation method of cordyceps militaris and radix puerariae peptide nutrition powder
CN105815449A (en) * 2016-03-23 2016-08-03 中南民族大学 Nostoc health care yoghourt and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2169472C2 (en) * 2000-06-19 2001-06-27 Цинберг Марк Беньяминович Method of preparing bacterial ferment for fermented-milk product
CN105815449A (en) * 2016-03-23 2016-08-03 中南民族大学 Nostoc health care yoghourt and preparation method thereof
CN105815777A (en) * 2016-03-29 2016-08-03 陕西富士金医药保健品有限责任公司 Preparation method of cordyceps militaris and radix puerariae peptide nutrition powder

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
徐婧君等: "植物乳杆菌CGMCC8198发酵果蔬奶的制备工艺研究", 《食品工业》 *
李安等: "桑椹五味子复合功能性酸奶的研制", 《食品工业》 *
李郭浪等: ""山药山楂酸奶的贮藏品质及胃肠液环境下的抗氧化性"", 《中国酿造》 *

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Application publication date: 20200918

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