CN111662710A - Boron-nitrogen double-doped blue fluorescent carbon quantum dot and preparation method and application thereof - Google Patents

Boron-nitrogen double-doped blue fluorescent carbon quantum dot and preparation method and application thereof Download PDF

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CN111662710A
CN111662710A CN202010542483.3A CN202010542483A CN111662710A CN 111662710 A CN111662710 A CN 111662710A CN 202010542483 A CN202010542483 A CN 202010542483A CN 111662710 A CN111662710 A CN 111662710A
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carbon quantum
boron
blue fluorescent
nitrogen double
fluorescent carbon
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CN111662710B (en
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闫娅楠
宋胜梅
孟雅婷
张慧林
双少敏
董川
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Shanxi University
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Abstract

The invention provides a boron and nitrogen double-doped blue fluorescent carbon quantum dot and a preparation method and application thereof. The preparation method comprises the following steps: dissolving sodium tetraborate and polyethyleneimine in water, and reacting at 160-200 ℃ for 4-10 hours to synthesize blue fluorescent carbon dots. The method for preparing the carbon dots has the advantages of simple process, wide raw material source, convenient price popularization, low preparation condition requirement and higher quantum yield of the obtained carbon quantum dots. The prepared carbon quantum dots can be used as fluorescent probes for ratio detection of morin.

Description

Boron-nitrogen double-doped blue fluorescent carbon quantum dot and preparation method and application thereof
Technical Field
The invention relates to a carbon luminescent nano material, in particular to a carbon quantum dot, and specifically provides a boron and nitrogen double-doped blue fluorescent carbon quantum dot and a preparation method and application thereof.
Background
Morin, 3,5,7,2 ', 4' -pentahydroxyflavone, (3,5,7,2 ', 4' -quercetin), is a pale yellow pigment extracted from the bark of plants of the Moraceae family such as ramulus mori, Citrus sinensis and many Chinese herbal medicines. Belongs to progesterone compounds. Has effects in inhibiting enzyme activity, resisting oxidation, relieving pain, resisting bacteria, relieving inflammation, resisting atherosclerosis, reducing blood sugar, and resisting stress. Can also be used as preoperative medicine or combined chemotherapy, and can improve curative effect and reduce side effect when being combined with other chemotherapy medicines. Therefore, the construction of the morin detection method is beneficial to monitoring the content of the morin in food and the metabolism condition in a human body. The existing detection methods, namely liquid chromatography and gas chromatography, are long in time consumption and low in detection sensitivity.
The unique structure and photophysical characteristics of the nano material open up a new path for fluorescence analysis. The carbon quantum dots are important members in fluorescent nano materials and are novel fluorescent carbon-based zero-dimensional materials. Because of its high stability, low toxicity, simple synthesis method and other characteristics, it attracts the extensive attention of researchers. The most important property is excellent fluorescence. Therefore, it can be applied to the fields of biomedicine, optoelectronics, catalysis, sensing and the like. The carbon spot sensitive to morin generally depends on the change in the intensity of single-wavelength fluorescence, which is inevitably affected by many factors, including fluctuations in probe concentration, excitation source, and surrounding environment, so the detection accuracy may be reduced. And ratio detection can improve the reliability and accuracy of the results. Therefore, it is of great significance to develop fluorescent carbon dots with rate characteristics.
Disclosure of Invention
The invention aims to provide a boron and nitrogen double-doped blue fluorescent carbon quantum dot and a preparation method thereof, and the carbon quantum dot can be used for ratio detection of morin. The carbon dots are simple in preparation process, wide in raw material source, convenient to popularize, low in preparation condition requirement, environment-friendly, capable of being synthesized in a common laboratory and easy to popularize.
The invention provides a preparation method of boron and nitrogen double-doped blue fluorescent carbon quantum dots, which comprises the following steps:
mixing sodium tetraborate and polyethyleneimine, and adding deionized water; transferring the solution into an autoclave, and reacting for 4-10 hours at 160-200 ℃; cooling to room temperature, filtering the product with filter paper to remove macromolecular particles, and dialyzing the obtained solution in deionized water for 8h through a 500Da dialysis membrane; finally, drying the carbon quantum dots in vacuum to collect target carbon quantum dots; the mass ratio of the sodium tetraborate to the polyethylene glycol to the deionized water is 0.02-0.1: 2.02-2.07: 20.
the mass ratio of the sodium tetraborate to the polyethylene glycol to the deionized water is preferably 0.06-0.1: 2.05-2.07: 20, more preferably 0.08 to 0.1: 2.06: 20.
the reaction temperature is preferably 180-200 ℃, and the reaction time is preferably 8-10 hours.
The carbon quantum dots prepared by the method can be used for detecting morin in urine as a fluorescent probe according to a formula cmin＝3SbThe lowest detection limit was found to be 0.10323. mu.M for the/S, with a linear range of 0.005-0.23. mu.M.
The invention has the advantages and effects that:
the carbon quantum dot solution can be obtained by a one-step hydrothermal method, the synthesis method is simple and effective, the raw materials are cheap and easy to obtain, the reaction conditions are mild, the environment is friendly, the reaction can be completed in a common laboratory, and the method is easy to popularize. The prepared carbon quantum dots can be used as probes for detecting morin in urine.
Drawings
Fig. 1 is an ultraviolet absorption spectrum and a fluorescence emission spectrum of the carbon quantum dot prepared in example 1.
Fig. 2 is an infrared spectrum of the carbon quantum dots prepared in example 1, in which the abscissa is the detection wavelength and the ordinate is the transmittance.
FIG. 3 is a fluorescence spectrum of carbon quantum dots prepared in example 1.
FIG. 4 is a linear fitting curve of morin concentration in the range of 0.005 μ M to 0.23 μ M.
FIG. 5 is a spectrum diagram of a carbon quantum dot fluorescence emission curve according to the variation of excitation wavelength prepared in example 1.
FIG. 6 is a spectrum of the carbon quantum dots prepared in example 1 for detecting morin in an actual sample.
Detailed Description
The present invention will be further described with reference to the following drawings and specific examples, which show detailed embodiments and specific procedures, but the scope of the present invention is not limited to the following examples.
Example 1
Step 1, dissolving 0.1000g of sodium tetraborate and 2mL of polyethyleneimine in 20mL of water at room temperature, fully stirring, and performing ultrasonic treatment to obtain a clear solution.
Step 2, the solution was transferred to a 50ml hydrothermal reaction kettle.
And 3, placing the hydrothermal kettle in an oven, and reacting for 8 hours at 200 ℃.
And 4, filtering the product by using filter paper to remove macromolecular particles, and dialyzing the obtained solution in deionized water for 8 hours by using a 500Da dialysis membrane.
And 5, freeze-drying the aqueous solution of the fluorescent carbon quantum dots to obtain the fluorescent carbon quantum dots, wherein the relative quantum yield (based on quinine sulfate) of the fluorescent carbon quantum dots is 11.17%.

Claims (6)

1. A preparation method of boron-nitrogen double-doped blue fluorescent carbon quantum dots is characterized by comprising the following steps:
mixing sodium tetraborate and polyethyleneimine, and adding deionized water; transferring the solution into an autoclave, and reacting for 4-10 hours at 160-200 ℃; cooling to room temperature, filtering the product with filter paper to remove macromolecular particles, and dialyzing the obtained solution in deionized water for 8h through a 500Da dialysis membrane; finally, drying the carbon quantum dots in vacuum to collect target carbon quantum dots; the mass ratio of the sodium tetraborate to the polyethylene glycol to the deionized water is 0.02-0.1: 2.02-2.07: 20.
2. the method for preparing boron-nitrogen double-doped blue fluorescent carbon quantum dots according to claim 1, wherein the mass ratio of the sodium tetraborate to the polyethylene to the deionized water is 0.06-0.1: 2.05-2.07: 20.
3. the method for preparing boron-nitrogen double-doped blue fluorescent carbon quantum dots according to claim 2, wherein the mass ratio of the sodium tetraborate to the polyethylene to the deionized water is 0.08-0.1: 2.06: 20.
4. the method for preparing the boron-nitrogen double-doped blue fluorescent carbon quantum dot according to claim 1, wherein the reaction temperature is 180-200 ℃ and the reaction time is 8-10 hours.
5. The boron-nitrogen double-doped blue fluorescent carbon quantum dot prepared by the method of any one of claims 1 to 4.
6. The application of the boron-nitrogen double-doped blue fluorescent carbon quantum dot in detecting urine morin according to claim 5.
CN202010542483.3A 2020-06-15 2020-06-15 Boron-nitrogen double-doped blue fluorescent carbon quantum dot and preparation method and application thereof Active CN111662710B (en)

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CN113480731A (en) * 2021-07-22 2021-10-08 西安工业大学 Detectable Cu2+And ClO-Preparation method of fluorescent carbon dot and hydrogel
CN114854403A (en) * 2022-04-21 2022-08-05 山西大学 Orange fluorescent carbon dot and preparation method and application thereof

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Publication number Priority date Publication date Assignee Title
CN113480731A (en) * 2021-07-22 2021-10-08 西安工业大学 Detectable Cu2+And ClO-Preparation method of fluorescent carbon dot and hydrogel
CN114854403A (en) * 2022-04-21 2022-08-05 山西大学 Orange fluorescent carbon dot and preparation method and application thereof

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