CN1116600C - Cell dehydrator and its dehydration method - Google Patents
Cell dehydrator and its dehydration method Download PDFInfo
- Publication number
- CN1116600C CN1116600C CN 00125723 CN00125723A CN1116600C CN 1116600 C CN1116600 C CN 1116600C CN 00125723 CN00125723 CN 00125723 CN 00125723 A CN00125723 A CN 00125723A CN 1116600 C CN1116600 C CN 1116600C
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- China
- Prior art keywords
- dehydrator
- cell
- rotating disk
- ultrasonic transducer
- paraffin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/14—Drying
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- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a cell dehydrator and a dehydration method thereof. The dehydration method comprises: soaking biological tissues needing to be treated in a treatment solution of alcohol, acetone, xylene, liquid paraffin, etc., and then placing the biological tissues in the reverberation field emitted by an ultrasonic transducer; placing a plurality of liquid storage tanks on the rotary disc of the dehydrator; connecting the liquid storage tanks with a closed container through flexible pipes and seal joints; replacing the liquid storage tanks by the rotary disc, a conveying arm and by changing over the sealing joints. By the action of the reverberation field emitted by the ultrasonic transducer, the dehydrator of the present invention can automatically and quickly replace intracellular water with paraffin in a full automatic closed mode; therefore, the treatment process of biological tissues of cells is effectively shortened, the labor intensity of operators is reduced, and the reliability of the working process is improved.
Description
Technical field:
The present invention relates to a kind of cell dehydrator and dewatering thereof, relate in particular to a kind of dewaterer and the dewatering thereof that can realize replacing ICW automatically fast, belong to field of biomedicine technology with paraffin.
Background technology:
Because fractographic needs, the section of animal and plant tissue must be very thin, general 3-5 micron, and this just need remove these in-house moisture, replaces paraffin.The classic method of this process is alcohol bubble three to five roads with variable concentrations, per pass 1～2 hour, separate out the moisture in the tissue, using then with alcohol has compatibility to have the intermediate solution of compatibility to soak two to three roads with paraffin again, time and alcohol are similar, to organize two to three road each a few hours of paraffin of immersing molten at last, paraffin is fully entered in the cell membrane, replace above-mentioned intermediate solution.Road surplus the whole operation ten, consuming time one to a couple of days.
In order to shorten processing procedure, the domestic methods of heating that adopt are about to above-mentioned solution and place baking oven to be incubated, but need the time more.For procedure robotization surplus making ten, two kinds of methods are arranged substantially: 1, tissue is placed on the revoliving arm, immerse each jar liquid successively abroad.As the RH-12EP of Japanese SAKURA board and the Citadel type of RH-12EPII and Britain SHANDON company.2, with in vacuum pump the container that fills biological tissue that each jar liquid suction successively one is fixing.Hyporcenter xp as Britain SHANDON company.These two kinds of automated methods all can only replace manual operations, can not shorten the processing time, can not shorten the cycle that the doctor goes out survey report.Though the method that adopts positive negative pressure to replace is arranged, produces little effect.Wherein first method can only be handled at normal temperatures, and can not accomplish good sealing, and solution evaporation spreads in the air to indoor, influences operating personnel's health.Second method has several tubes and tens valves, place gas leakage, and then complete machine can not be worked.In addition, these two kinds of methods also have a kind of potential danger, reached one day or the processing procedure at a night in, very difficult assurance has operating personnel on the scene all the time, if meet outage, then institute in a organized way (100 multiple-cases sometimes) scrap, even produce irremediable serious consequence.
Summary of the invention:
The objective of the invention is to above-mentioned deficiency at prior art, a kind of new cell dehydrator and dewatering thereof are provided, can not only shorten the processing procedure of biological tissue greatly, provide the pathological section report early, help in time curing the patient, can also therefore improve operational reliability.
For realizing such purpose, cell dehydrator in the technical solution of the present invention comprises a rotating disk, places several fluid reservoirs on the rotating disk, and fluid reservoir links to each other with closed container with sealing adaptor by flexible tube, transmission arm is arranged on the rotating disk in addition, can drive fluid reservoir and move up and down.Except the solution of handling biological tissue, also be equipped with ultrasonic transducer in the closed container.
The cell dehydration method that the present invention adopts is full automatic air-tight manner, automatically realize replacing ICW with paraffin, the biological tissue of required processing is immersed in the solution such as alcohol, acetone, dimethylbenzene, liquid paraffin, and it is in the reverberation field that ultrasonic transducer sends.Because ultrasound wave is more or less the same in sound's velocity in liquid and biological tissue, so deep enough organization internal of acoustic energy, make cell membrane make dither, thereby increase the permeability of cell membrane, make the outer solution of cell membrane can separate out the interior liquid of film quickly, comprise moisture and residual not with the affine liquid of paraffin, paraffin is immersed in the dehydrated cell.In order to change these liquid, under airtight container, open a hole, by special changeable sealing adaptor and flexible tube, link to each other with some fluid reservoirs.When transmission arm drove fluid reservoir and changes the position up and down, liquid flow to because of deadweight or flows out closed container.A fluid reservoir places on the rotating disk surplus in the of ten, by the rotation of rotating disk, the drive and the switching sealing adaptor of transferring arm, finishes the replacing of fluid reservoir.
The reverberation field effect that the present invention utilizes ultrasonic transducer to send, and the automatic replacing by fluid reservoir, can be with the processing procedure of biological tissue, comprise fixing, dehydration, transparent, waxdip, shorten to two hours by a whole day (reaching two, three days sometimes), if the size of section and THICKNESS CONTROL are within the specific limits, also can be by improving method of temperature, overall process was shortened to about half an hour, near the required time of freezing microtome section, help providing early the pathological section report, in time cure the patient, its treatment capacity adapts to the needs of general large hospital.Description of drawings and embodiment:
For understanding technical scheme of the present invention better, be further described below in conjunction with accompanying drawing.
Fig. 1 is a dewaterer apparatus structure synoptic diagram of the present invention.
As shown in the figure, place several fluid reservoirs 4 on the rotating disk 5 of cell dehydrator, fluid reservoir 4 links to each other with closed container 1 with changeable sealing adaptor 2 by flexible tube 3, and transmission arm 6 is arranged on the rotating disk 5 in addition, can drive fluid reservoir 4 and move up and down.Treatment Solution 8 such as alcohol, acetone, dimethylbenzene, liquid paraffin are arranged in the closed container 1, and pending biological tissue 7 is immersed in these Treatment Solution 8, also is equipped with ultrasonic transducer 9 in the closed container 1.
The present invention adopts full automatic air-tight manner, automatically realize replacing ICW with paraffin, the biological tissue 7 of required processing is immersed in the Treatment Solution 8 such as alcohol, acetone, dimethylbenzene, liquid paraffin, and it is in the reverberation field that ultrasonic transducer 9 sends.A plurality of fluid reservoirs 4 place on the rotating disk 5, when rotating disk 5 runs to certain position, driving fluid reservoir 4 by transferring arm 6 rises, make the liquid in the fluid reservoir 4 enter closed container 1 by sealing adaptor 2 and flexible tube 3, after finishing, this phase process is returned on the rotating disk 5 by transferring arm 6 drives, when rotating disk 5 forwards certain position to, drive another fluid reservoir 4 again by transmission arm 6 and rise, finish the process of changing liquid.
The present invention has effectively shortened the processing procedure of cell biological tissue, can shorten interval between diagnosis, alleviates labor intensity of operating personnel; The entire process process does not have harmful gas and leaks, and because the cell dehydration processing time is shorter, can guarantee that operating personnel are on the scene, and reason power failure or other reasons are scrapped a large amount of tissues.Adopt single flexible tube in the cell dehydrator,, improved the reliability of complete machine without valve.
Claims (2)
1, a kind of cell dehydrator, it is characterized in that upward placement a plurality of fluid reservoirs (4) of rotating disk (5), described fluid reservoir (4) links to each other with closed container (1) with sealing adaptor (2) by flexible tube (3), transmission arm (6) is arranged on the rotating disk (5) in addition, Treatment Solution (8) is arranged in the closed container (1) and be equipped with ultrasonic transducer (9).
2, a kind of cell dehydration method, it is characterized in that adopting full automatic air-tight manner, the biological tissue (7) of required processing is immersed in alcohol, acetone, dimethylbenzene, the liquid paraffin Treatment Solution (8), and it is in the reverberation field that ultrasonic transducer (9) sends, a plurality of fluid reservoirs (4) place on the rotating disk (5), finish the replacing of fluid reservoir (4) by the rotation of rotating disk (5), the drive and the switching sealing adaptor (2) of transferring arm (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00125723 CN1116600C (en) | 2000-10-19 | 2000-10-19 | Cell dehydrator and its dehydration method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00125723 CN1116600C (en) | 2000-10-19 | 2000-10-19 | Cell dehydrator and its dehydration method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1292494A CN1292494A (en) | 2001-04-25 |
| CN1116600C true CN1116600C (en) | 2003-07-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00125723 Expired - Fee Related CN1116600C (en) | 2000-10-19 | 2000-10-19 | Cell dehydrator and its dehydration method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1116600C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100376457C (en) * | 2003-12-12 | 2008-03-26 | 欧洲直升机公司 | Modular de-icing device for an aerodynamic surface |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101923018B (en) * | 2010-09-15 | 2013-01-23 | 福州大学 | Method for dehydrating using biological tissue dehydrator |
-
2000
- 2000-10-19 CN CN 00125723 patent/CN1116600C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100376457C (en) * | 2003-12-12 | 2008-03-26 | 欧洲直升机公司 | Modular de-icing device for an aerodynamic surface |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1292494A (en) | 2001-04-25 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |