CN111658672A - Human placenta stem cell extract freeze-dried powder and preparation method thereof - Google Patents
Human placenta stem cell extract freeze-dried powder and preparation method thereof Download PDFInfo
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- CN111658672A CN111658672A CN202010569631.0A CN202010569631A CN111658672A CN 111658672 A CN111658672 A CN 111658672A CN 202010569631 A CN202010569631 A CN 202010569631A CN 111658672 A CN111658672 A CN 111658672A
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- stem cell
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- dried powder
- cell extract
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
Abstract
The invention discloses a human placenta stem cell extract freeze-dried powder and a preparation method thereof, in particular to a preparation method of a human placenta stem cell extract freeze-dried powder, which comprises the following steps: s1, culturing human placenta mesenchymal stem cells by using a cell culture medium containing serum with a pH value of 5.6-6.8 until 70-90% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution; s2, standing the mixed solution in an environment with a pH value of 5.6-6.8, and sterilizing at high temperature after standing; s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃; s4 filtration was performed using precision filter paper. The human placental stem cell freeze-dried powder has the effects of comprehensively regulating an aged organism and recovering young; it can greatly raise metabolism level of organism, raise physiological function, raise immunity of organism, prolong puberty period, beautify skin, prolong life and delay senility, and at the same time, the freeze-dried powder is long in storage period, not easy to degrade and convenient for use.
Description
Technical Field
The invention relates to the technical field of freeze-dried powder preparation, in particular to human placenta stem cell extract freeze-dried powder and a preparation method thereof.
Background
The human placenta stem cell extract is freeze dried powder prepared with human placenta stem cell cultured to secrete bioactive matters including protein, polypeptide, cell growth factor, bioactive factor and other components and through special separation and extraction process and processing. The multiple active components in the human placental stem cell extract have complex physiological effects, each component can affect a plurality of tissues and organs, and the improvement of the physiological function of each form is not the effect of a single factor but the mutual coordination effect of a plurality of factors is needed, so the human placental stem cell extract has better effects of resisting aging and activating the body functions than the single cytokine. The placenta stem cell extract has effects of caring skin, resisting aging, activating organism function, regenerating cell, repairing ultraviolet injury, and improving skin immunity.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a human placental stem cell extract freeze-dried powder and a preparation method thereof.
The invention provides a human placenta stem cell extract freeze-dried powder and a preparation method thereof, comprising the following steps:
s1, culturing human placenta mesenchymal stem cells by using a cell culture medium containing serum with a pH value of 5.6-6.8 until 70-90% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution;
s2, standing the mixed solution in an environment with a pH value of 5.6-6.8, and sterilizing at high temperature after standing;
s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃;
s4, filtering by using precision filter paper;
s5 filling the solution after precision filtration into a bottle, covering the bottle stopper, filling into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.
Preferably, the room temperature is maintained at 20 to 25 degrees during the preparation.
Preferably, the cell culture medium is DMEM/F12 medium, and the nutrient medium contains antibacterial antibiotics.
Preferably, the precision filter paper of step S4 is a filter membrane with a pore size of 0.45 μm.
A freeze-dried powder of human placental stem cell extract comprises the freeze-dried powder obtained by the preparation method.
In the invention, the human placental stem cell extract freeze-dried powder contains various life active substances, has extremely high excitation effect on cell division and proliferation of an organism, and has the effects of comprehensively regulating an aged organism and recovering young; the placenta stem cell extract freeze-dried powder prepared by the special process has the advantages of long storage period, difficult degradation and convenient use.
The human placental stem cell freeze-dried powder has the effects of comprehensively regulating an aged organism and recovering young; it can greatly raise metabolism level of organism, raise physiological function, raise immunity of organism, prolong puberty period, beautify skin, prolong life and delay senility, and at the same time, the freeze-dried powder is long in storage period, not easy to degrade and convenient for use.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example one
A freeze-dried powder of human placental stem cell extract and a preparation method thereof comprise the following steps:
s1, when the human placenta mesenchymal stem cells are cultured by using a cell culture medium with a pH value of 5.6 and containing serum until 70% of cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution;
s2, standing the mixed solution in an environment with a pH value of 5.6, and sterilizing the mixed solution at high temperature after standing;
s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2 ℃;
s4, filtering by using precision filter paper;
s5 filling the solution after precision filtration into a bottle, covering the bottle stopper, filling into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.
In the present invention, the room temperature was maintained at 20 degrees during the preparation.
In the invention, the cell culture medium is DMEM/F12 culture medium, and the culture medium contains antibacterial antibiotics.
In the present invention, the precision filter paper of step S4 is a filter membrane with a pore size of 0.45 μm.
A freeze-dried powder of human placental stem cell extract comprises the freeze-dried powder obtained by the preparation method.
Example two
A freeze-dried powder of human placental stem cell extract and a preparation method thereof comprise the following steps:
s1, culturing human placenta mesenchymal stem cells by using a cell culture medium with a pH value of 6.2 and containing serum until 80% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution;
s2, standing the mixed solution in an environment with a pH value of 6.2, and sterilizing the mixed solution at high temperature after standing;
s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃;
s4, filtering by using precision filter paper;
s5 filling the solution after precision filtration into a bottle, covering the bottle stopper, filling into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.
In the present invention, the room temperature was kept at 22 degrees during the preparation.
In the invention, the cell culture medium is DMEM/F12 culture medium, and the culture medium contains antibacterial antibiotics.
In the present invention, the precision filter paper of step S4 is a filter membrane with a pore size of 0.45 μm.
A lyophilized powder of human placental stem cell extract comprises lyophilized powder obtained by the above preparation method
EXAMPLE III
A freeze-dried powder of human placental stem cell extract and a preparation method thereof comprise the following steps:
s1, culturing human placenta mesenchymal stem cells by using a cell culture medium with a pH value of 6.8 and containing serum until 90% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution;
s2, standing the mixed solution in an environment with a pH value of 6.8, and sterilizing the mixed solution at high temperature after standing;
s3, cooling the mixed solution after high-temperature disinfection and sterilization to 5 ℃;
s4, filtering by using precision filter paper;
s5 filling the solution after precision filtration into a bottle, covering the bottle stopper, filling into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.
In the present invention, the room temperature was kept at 25 degrees during the preparation.
In the invention, the cell culture medium is DMEM/F12 culture medium, and the culture medium contains antibacterial antibiotics.
In the present invention, the precision filter paper of step S4 is a filter membrane with a pore size of 0.45 μm.
A lyophilized powder of human placental stem cell extract comprises lyophilized powder obtained by the above preparation method
The invention comprises the following steps: culturing human placenta mesenchymal stem cells by using a cell culture medium with a pH value of 5.6-6.8 and containing serum until 70-90% of the cells are fused, adding deionized water, a stabilizer and an excipient, and mixing and dissolving until the mixture is transparent to obtain a mixed solution; standing the mixed solution in an environment with a pH value of 5.6-6.8, and sterilizing at high temperature after standing; cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃; filtering by adopting precise filter paper; and (3) filling the solution after the precision filtration into a bottle, covering the bottle stopper, dishing into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. A preparation method of human placental stem cell extract freeze-dried powder is characterized by comprising the following steps:
s1, culturing human placenta mesenchymal stem cells by using a cell culture medium containing serum with a pH value of 5.6-6.8 until 70-90% of the cells are fused, adding deionized water, a stabilizer and an excipient, mixing and dissolving until the mixture is transparent to obtain a mixed solution;
s2, standing the mixed solution in an environment with a pH value of 5.6-6.8, and sterilizing at high temperature after standing;
s3, cooling the mixed solution after high-temperature disinfection and sterilization to 2-5 ℃;
s4, filtering by using precision filter paper;
s5 filling the solution after precision filtration into a bottle, covering the bottle stopper, filling into a freeze-drying device, standing for 60 minutes, defoaming, and grinding to obtain the human placenta stem cell extract freeze-dried powder.
2. The method for preparing lyophilized powder of human placental stem cell extract according to claim 1, wherein the room temperature is maintained at 20-25 degrees during the preparation.
3. The method for preparing lyophilized powder of human placental stem cell extract according to claim 1, wherein the cell culture medium is DMEM/F12 culture medium, and the culture medium contains antibacterial antibiotics.
4. The method of claim 1, wherein the precision filter paper of step S4 is a filter membrane with a pore size of 0.45 μm.
5. A lyophilized powder of human placental stem cell extract comprising the lyophilized powder obtained by the preparation method of claims 1 to 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010569631.0A CN111658672A (en) | 2020-06-20 | 2020-06-20 | Human placenta stem cell extract freeze-dried powder and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010569631.0A CN111658672A (en) | 2020-06-20 | 2020-06-20 | Human placenta stem cell extract freeze-dried powder and preparation method thereof |
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| CN111658672A true CN111658672A (en) | 2020-09-15 |
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| CN202010569631.0A Pending CN111658672A (en) | 2020-06-20 | 2020-06-20 | Human placenta stem cell extract freeze-dried powder and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6372494B1 (en) * | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| CN102600057A (en) * | 2012-03-16 | 2012-07-25 | 广州赛莱拉生物科技有限公司 | Human placenta stem cell extractive lyophilized powder as well as preparation method thereof and application thereof |
| CN111228134A (en) * | 2020-03-03 | 2020-06-05 | 广州鸿懿实业发展有限公司 | Snake venom peptide active freeze-dried powder and preparation method thereof |
-
2020
- 2020-06-20 CN CN202010569631.0A patent/CN111658672A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6372494B1 (en) * | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| CN102600057A (en) * | 2012-03-16 | 2012-07-25 | 广州赛莱拉生物科技有限公司 | Human placenta stem cell extractive lyophilized powder as well as preparation method thereof and application thereof |
| CN111228134A (en) * | 2020-03-03 | 2020-06-05 | 广州鸿懿实业发展有限公司 | Snake venom peptide active freeze-dried powder and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| MAIRA S OLIVEIRA 等: "\"Placental-derived stem cells: Culture, differentiation and challenges\"" * |
| MAIRA S OLIVEIRA 等: ""Placental-derived stem cells: Culture, differentiation and challenges"" * |
| 徐海环 等: ""脐带间充质干细胞上清冻干粉对大耳兔皮肤缺损的治疗作用"" * |
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