CN111650306A - HPLC method for simultaneously determining eight effective components in pill of six ingredients with rehmannia - Google Patents
HPLC method for simultaneously determining eight effective components in pill of six ingredients with rehmannia Download PDFInfo
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- CN111650306A CN111650306A CN202010648284.0A CN202010648284A CN111650306A CN 111650306 A CN111650306 A CN 111650306A CN 202010648284 A CN202010648284 A CN 202010648284A CN 111650306 A CN111650306 A CN 111650306A
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- 238000004128 high performance liquid chromatography Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000004615 ingredient Substances 0.000 title claims abstract description 18
- 239000006187 pill Substances 0.000 title claims abstract description 18
- 241000405414 Rehmannia Species 0.000 title claims abstract description 14
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 105
- YTZSBJLNMIQROD-SFBCHFHNSA-N Morroniside Chemical compound O([C@@H]1OC=C([C@H]2C[C@H](O)O[C@@H](C)[C@H]21)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YTZSBJLNMIQROD-SFBCHFHNSA-N 0.000 claims abstract description 42
- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical compound COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 claims abstract description 42
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims abstract description 40
- 239000000243 solution Substances 0.000 claims abstract description 25
- YTZSBJLNMIQROD-UHFFFAOYSA-N (4aS)-1c-beta-D-glucopyranosyloxy-6xi-hydroxy-8t-methyl-(4ar,8ac)-5,6,8,8a-tetrahydro-1H,4aH-pyrano[3,4-c]pyran-4-carboxylic acid methyl ester Natural products C12C(C)OC(O)CC2C(C(=O)OC)=COC1OC1OC(CO)C(O)C(O)C1O YTZSBJLNMIQROD-UHFFFAOYSA-N 0.000 claims abstract description 21
- AMBQHHVBBHTQBF-UHFFFAOYSA-N Loganin Natural products C12C(C)C(O)CC2C(C(=O)OC)=COC1OC1OC(CO)C(O)C(O)C1O AMBQHHVBBHTQBF-UHFFFAOYSA-N 0.000 claims abstract description 21
- YLTGFGDODHXMFB-UHFFFAOYSA-N isoacetovanillon Natural products COC1=CC=C(C(C)=O)C=C1O YLTGFGDODHXMFB-UHFFFAOYSA-N 0.000 claims abstract description 21
- AMBQHHVBBHTQBF-UOUCRYGSSA-N loganin Chemical compound O([C@@H]1OC=C([C@H]2C[C@H](O)[C@H](C)[C@H]21)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O AMBQHHVBBHTQBF-UOUCRYGSSA-N 0.000 claims abstract description 21
- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 claims abstract description 21
- NOEGNKMFWQHSLB-UHFFFAOYSA-N 5-hydroxymethylfurfural Chemical compound OCC1=CC=C(C=O)O1 NOEGNKMFWQHSLB-UHFFFAOYSA-N 0.000 claims abstract description 20
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 claims abstract description 20
- OZWDYLLMBFLSNH-UHFFFAOYSA-N Swertisin Natural products COc1cc2OC(=CC(=O)c2c(O)c1OC3OC(CO)C(O)C(O)C3O)c4cccc(O)c4 OZWDYLLMBFLSNH-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229940074391 gallic acid Drugs 0.000 claims abstract description 20
- 235000004515 gallic acid Nutrition 0.000 claims abstract description 20
- RJGBSYZFOCAGQY-UHFFFAOYSA-N hydroxymethylfurfural Natural products COC1=CC=C(C=O)O1 RJGBSYZFOCAGQY-UHFFFAOYSA-N 0.000 claims abstract description 20
- YKRGDOXKVOZESV-UHFFFAOYSA-N paeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(CO)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-UHFFFAOYSA-N 0.000 claims abstract description 20
- ABRULANJVVJLFI-DGHBBABESA-N swertisin Chemical compound COC1=CC=2OC(C=3C=CC(O)=CC=3)=CC(=O)C=2C(O)=C1[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ABRULANJVVJLFI-DGHBBABESA-N 0.000 claims abstract description 20
- 239000013558 reference substance Substances 0.000 claims abstract description 18
- 238000005303 weighing Methods 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 239000012488 sample solution Substances 0.000 claims abstract description 15
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000007865 diluting Methods 0.000 claims abstract description 9
- 239000000706 filtrate Substances 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 238000000227 grinding Methods 0.000 claims abstract description 9
- 239000012088 reference solution Substances 0.000 claims abstract description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000010828 elution Methods 0.000 claims abstract description 8
- UGWAFWFSLCHCJD-UHFFFAOYSA-N Cornuside Natural products OC1C(O)C(O)C(CO)OC1OC1C(C=C)C(CCOC(=O)C=2C=C(O)C(O)=C(O)C=2)C(C(O)=O)=CO1 UGWAFWFSLCHCJD-UHFFFAOYSA-N 0.000 claims abstract description 6
- SMTKSCGLXONVGL-ZEIULZLQSA-N methyl (2r,3s,4r)-3-ethenyl-4-[2-(3,4,5-trihydroxybenzoyl)oxyethyl]-2-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,4-dihydro-2h-pyran-5-carboxylate Chemical compound O([C@H]1OC=C([C@@H]([C@@H]1C=C)CCOC(=O)C=1C=C(O)C(O)=C(O)C=1)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SMTKSCGLXONVGL-ZEIULZLQSA-N 0.000 claims abstract description 6
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 19
- 238000001514 detection method Methods 0.000 claims description 4
- 239000009220 Liuwei Dihuang Decoction Substances 0.000 claims 3
- 239000003814 drug Substances 0.000 abstract description 9
- 238000010998 test method Methods 0.000 abstract description 2
- 238000004090 dissolution Methods 0.000 description 11
- -1 cornusin Chemical compound 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 4
- 238000010992 reflux Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 3
- 238000003908 quality control method Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention provides an HPLC method for simultaneously determining eight effective components in a pill of six ingredients with rehmannia, which comprises the following steps: preparation of sample solution: taking a proper amount of samples, grinding, precisely weighing, placing into a measuring flask, adding part of 70% methanol, ultrasonically dissolving, diluting with 70% methanol to scale, shaking, filtering, and collecting filtrate; preparation of a reference solution: precisely weighing gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornuside, and paeonol as reference substances, and adding 70% methanol to obtain reference substance solution; the conditions of the high performance liquid chromatography are as follows: the C18 chromatographic column is adopted, 0.2% phosphoric acid solution is used as an A mobile phase, acetonitrile is used as a B mobile phase, and gradient elution is adopted. The test method is convenient, quick in connection, stable, precise, good in reproducibility and capable of effectively representing the overall quality of the medicine.
Description
Technical Field
The invention relates to an HPLC method for simultaneously determining eight effective components in a pill of six ingredients with rehmannia, belonging to the technical field of medicine quality control.
Background
The quality of traditional Chinese medicine products is mostly reflected by the content of single component or 2-3 components in the existing traditional Chinese medicine standard, but the traditional Chinese medicine components are complex and have the characteristics of multiple components and multiple targets, and the quality of the traditional Chinese medicine cannot be comprehensively reflected by only one or 2-3 components, so that the idea of multi-component quality control is provided, namely the quality of the traditional Chinese medicine is reflected by multi-index synchronous quality control, and the traditional Chinese medicine standard becomes the consensus of a plurality of experts and scholars.
The Chinese pharmacopoeia (2020 edition) only has three effective components of morroniside, loganin and paeonol as the content control indexes of the pill of six ingredients with rehmannia, and 654 approved characters are shared in China, and the basic requirements in the pharmacopoeia standard cannot distinguish the quality of each product, so that an HPLC method for simultaneously measuring a plurality of effective components in the pill of six ingredients with rehmannia is established, so that the quality of the pill of six ingredients with rehmannia can be better controlled to become the research direction of people.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide an HPLC method for simultaneously measuring eight effective components in the pill of six ingredients with rehmannia, which can better control the product quality of the pill of six ingredients with rehmannia.
In order to achieve the above object, the present invention provides an HPLC method for simultaneously determining eight effective ingredients in liuwei dihuang pills, which is characterized by comprising the following steps:
preparation of sample solution: preparation of sample solution: taking a proper amount of samples, grinding, precisely weighing, placing into a measuring flask, adding part of 70% methanol, ultrasonically dissolving, diluting with 70% methanol to scale, shaking, filtering, and collecting filtrate;
preparation of a reference solution: precisely weighing gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornuside, and paeonol as reference substances, and adding 70% methanol to obtain reference substance solution;
detecting the sample solution and the reference solution by adopting a high performance liquid chromatography to obtain qualitative and quantitative detection results of eight effective components, namely gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin and paeonol, in the pill of six ingredients with rehmannia, wherein the conditions of the high performance liquid chromatography are as follows: using a C18 chromatographic column, using a 0.2% phosphoric acid solution as an A mobile phase and acetonitrile as a B mobile phase, and adopting gradient elution, wherein the gradient elution procedure is as follows:
| T(min) | A％ | B％ |
| 0 | 95 | 5 |
| 5 | 92 | 8 |
| 15 | 92 | 8 |
| 30 | 75 | 25 |
| 40 | 35 | 65 |
| 50 | 35 | 65 |
| 51 | 5 | 95 |
| 60 | 5 | 95 |
| 61 | 95 | 5 |
| 66 | 95 | 5 |
in the scheme, the method comprises the following steps: the ultrasonic time is 1 h.
In the scheme, the method comprises the following steps: each 1ml of the control solution contains 30 μ g of gallic acid, 30 μ g of 5-hydroxymethylfurfural, 40 μ g of morroniside, 40 μ g of loganin, 30 μ g of swertisin, 30 μ g of paeoniflorin, 30 μ g of cornuside and 90 μ g of paeonol.
In the scheme, the method comprises the following steps: the column temperature is 28-32 ℃, the flow rate is 0.8-1.2mL/min, and the wavelength is 240 nm.
In the scheme, the method comprises the following steps: the mass concentration of the sample solution was 0.01g sample/ml methanol solution.
Has the advantages that: 1. the invention adopts a gradient elution procedure, can realize effective separation of eight components in the pill of six ingredients with rehmannia, and carries out qualitative and quantitative determination on each effective component.
2. The sample is dissolved by 70% of methanol, so that the good dissolution rate of the effective components and the good peak shape can be obtained, and the detection precision is higher.
3. The ultrasonic dissolution of the sample is adopted, and the dissolution rate is high.
4. The test method is convenient, quick in connection, stable, precise, good in reproducibility and capable of effectively representing the overall quality of the medicine.
Drawings
FIG. 1 is an HPLC chromatogram of a sample to be tested in example 1.
FIG. 2 is an HPLC chromatogram of a control of example 1.
FIG. 3 is an HPLC chromatogram of a sample to be tested in example 2.
FIG. 4 is an HPLC chromatogram of a sample to be tested in example 3.
FIG. 5 is an HPLC chromatogram of a sample to be tested in example 4.
FIG. 6 is an HPLC chromatogram of a sample to be tested of example 5.
FIG. 7 is an HPLC chromatogram of a sample to be tested of example 6.
The specific implementation mode is as follows:
the present invention will be described in further detail with reference to the following examples and the accompanying drawings.
Example 1
Preparation of sample solution: taking a proper amount of sample, grinding, taking about 0.5g, precisely weighing, placing into a 50ml measuring flask, adding 25ml of 70% (weight) methanol, ultrasonically dissolving for 1 hour, diluting with 70% methanol to scale, shaking up, filtering, and taking the filtrate.
Preparation of a reference solution: precisely weighing gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin, and paeonol as reference substances, and adding 70% methanol to obtain reference substance solution containing gallic acid 30 μ g, 5-hydroxymethyl furfural 30 μ g, morroniside 40 μ g, loganin 40 μ g, swertisin 30 μ g, paeoniflorin 30 μ g, cornusin 30 μ g, and paeonol 90 μ g per 1ml of reference substance solution.
Example 2
Preparation of sample solution: taking a proper amount of samples, grinding, taking about 0.5g, precisely weighing, placing in a 50ml measuring flask, adding 25ml of 100% methanol, dissolving by ultrasonic for 1 hour, diluting to scale with 100% methanol, shaking up, filtering, and taking the filtrate.
Preparation of a reference solution: precisely weighing gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin, and paeonol as reference substances, and adding 100% methanol to obtain reference substance solution containing gallic acid 30 μ g, 5-hydroxymethyl furfural 30 μ g, morroniside 40 μ g, loganin 40 μ g, swertisin 30 μ g, paeoniflorin 30 μ g, cornusin 30 μ g, and paeonol 90 μ g per 1ml of reference substance solution.
Example 3
Preparation of sample solution: taking a proper amount of samples, grinding, taking about 0.5g, precisely weighing, placing in a 50ml measuring flask, adding 25ml of 50% methanol, dissolving by ultrasonic for 1 hour, diluting to scale with 50% methanol, shaking up, filtering, and taking the filtrate.
Preparation of a reference solution: precisely weighing gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin, and paeonol as reference substances, and adding 50% methanol to obtain reference substance solution containing gallic acid 30 μ g, 5-hydroxymethyl furfural 30 μ g, morroniside 40 μ g, loganin 40 μ g, swertisin 30 μ g, paeoniflorin 30 μ g, cornusin 30 μ g, and paeonol 90 μ g per 1ml of reference substance solution.
Example 4
Preparation of sample solution: taking a proper amount of sample, grinding, taking about 0.5g, precisely weighing, placing in a 50ml measuring flask, adding 25ml of 50% methanol, heating and refluxing for 1 hour, diluting to scale with methanol, shaking uniformly, filtering, and taking a subsequent filtrate.
Control solution: taking appropriate amount of gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin, and paeonol reference substances, precisely weighing, and adding 50% methanol to obtain a solution containing gallic acid 30 μ g, 5-hydroxymethyl furfural 30 μ g, morroniside 40 μ g, loganin 40 μ g, swertisin 30 μ g, paeoniflorin 30 μ g, cornusin 30 μ g, and paeonol 90 μ g per 1 ml.
Scheme 5:
preparation of sample solution: taking a proper amount of sample, grinding, taking about 0.5g, precisely weighing, placing in a 50ml measuring flask, adding 25ml of 100% methanol, heating and refluxing for 1 hour, diluting to scale with methanol, shaking uniformly, filtering, and taking a subsequent filtrate.
Control solution: taking appropriate amount of gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin, and paeonol reference substances, precisely weighing, and adding 100% methanol to obtain a solution containing gallic acid 30 μ g, 5-hydroxymethyl furfural 30 μ g, morroniside 40 μ g, loganin 40 μ g, swertisin 30 μ g, paeoniflorin 30 μ g, cornusin 30 μ g, and paeonol 90 μ g per 1 ml.
Scheme 6:
preparation of sample solution: taking a proper amount of sample, grinding, taking about 0.5g, precisely weighing, placing in a 50ml measuring flask, adding 25ml of 50% methanol, heating and refluxing for 1 hour, diluting to scale with methanol, shaking uniformly, filtering, and taking a subsequent filtrate.
Control solution: taking appropriate amount of gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin, and paeonol reference substances, precisely weighing, and adding 50% methanol to obtain a solution containing gallic acid 30 μ g, 5-hydroxymethyl furfural 30 μ g, morroniside 40 μ g, loganin 40 μ g, swertisin 30 μ g, paeoniflorin 30 μ g, cornusin 30 μ g, and paeonol 90 μ g per 1 ml.
The samples used in examples 1-6 were all the same.
Detecting the sample solutions and the reference solution in the examples 1 to 6 by adopting a high performance liquid chromatography to obtain qualitative and quantitative detection results of eight effective components, namely gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornuside and paeonol, in the pill of six ingredients with rehmannia, wherein the conditions of the high performance liquid chromatography are as follows: adopting C18 chromatographic column, with column temperature of 28-32 deg.C, flow rate of 0.8-1.2mL/min, wavelength of 240nm, 0.2% phosphoric acid solution as A mobile phase, acetonitrile as B mobile phase, and gradient elution procedure as follows:
| T(min) | A％ | B％ |
| 0 | 95 | 5 |
| 5 | 92 | 8 |
| 15 | 92 | 8 |
| 30 | 75 | 25 |
| 40 | 35 | 65 |
| 50 | 35 | 65 |
| 51 | 5 | 95 |
| 60 | 5 | 95 |
| 61 | 95 | 5 |
| 66 | 95 | 5 |
according to analysis, the sample is dissolved by 70% of methanol, so that the good dissolution rate of the effective components and the good peak shape can be obtained, the system adaptability test is good, and the dissolution rate is high. By comparing the dissolution with the 100% methanol solution and the dissolution with the 50% methanol solution, it can be seen that when the 100% methanol solution is used to dissolve the sample, although the dissolution rate is better, the obtained peak shape is asymmetric, and the determination accuracy is poorer. The sample is dissolved by 50% methanol, and although the obtained peak shape is good, the component dissolution rate is low, which is not beneficial to the determination of the effective components in the sample. Under the condition of the gradient elution of the invention, the effective separation of 8 components can be realized, and under the condition of the wavelength of the invention, the 8 components have good absorption peak shapes. The ultrasonic dissolution of the sample is higher than the heating reflux dissolution rate.
The above description is for the purpose of describing the invention in more detail with reference to specific preferred embodiments, and it should not be construed that the embodiments of the invention are limited to those described herein, and it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention.
Claims (4)
1. An HPLC method for simultaneously determining eight effective components in a pill of six ingredients with rehmannia is characterized by comprising the following steps:
preparation of sample solution: taking a proper amount of samples, grinding, precisely weighing, placing into a measuring flask, adding part of 70% methanol, ultrasonically dissolving, diluting with 70% methanol to scale, shaking, filtering, and collecting filtrate;
preparation of a reference solution: precisely weighing gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornuside and paeonol as reference substances, and adding 70% methanol to obtain reference substance solution;
detecting the sample solution and the reference solution by adopting a high performance liquid chromatography to obtain qualitative and quantitative detection results of eight effective components, namely gallic acid, 5-hydroxymethyl furfural, morroniside, loganin, swertisin, paeoniflorin, cornusin and paeonol, in the pill of six ingredients with rehmannia, wherein the conditions of the high performance liquid chromatography are as follows: using a C18 chromatographic column, using a 0.2% phosphoric acid solution as an A mobile phase and acetonitrile as a B mobile phase, and adopting gradient elution, wherein the gradient elution procedure is as follows:
2. an HPLC method for simultaneously determining eight effective ingredients in LIUWEIDIHUANG pill according to claim 1, which is characterized in that: each 1ml of the control solution contains 30 μ g of gallic acid, 30 μ g of 5-hydroxymethylfurfural, 40 μ g of morroniside, 40 μ g of loganin, 30 μ g of swertisin, 30 μ g of paeoniflorin, 30 μ g of cornuside and 90 μ g of paeonol.
3. The HPLC method for simultaneously measuring eight effective ingredients in LIUWEIDIHUANG pill according to claim 1 or 2, wherein: the column temperature is 28-32 ℃, the flow rate is 0.8-1.2mL/min, and the wavelength is 240 nm.
4. An HPLC method for simultaneously determining eight effective ingredients in LIUWEIDIHUANG pill according to claim 1, which is characterized in that: the mass concentration of the sample solution was 0.01g sample/ml methanol.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115166081A (en) * | 2022-06-28 | 2022-10-11 | 鲁南厚普制药有限公司 | Method for measuring multi-component content of Yangchun oral liquid |
| CN115389663A (en) * | 2022-08-24 | 2022-11-25 | 上海和黄药业有限公司 | Method for measuring contents of various components in deafness zuoci pill mixed powder |
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| CN104865320A (en) * | 2015-03-31 | 2015-08-26 | 湖南中医药大学 | HPLC dual-wavelength fingerprint determination method for multiple components of six-ingredient glutinous rehmannia preparation |
| CN105784951A (en) * | 2014-12-24 | 2016-07-20 | 九芝堂股份有限公司 | Multiple indicator rapid detection method for raw medicinal powder of condensed pill of six drugs with rehmannia |
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|---|---|---|---|---|
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Application publication date: 20200911 |