CN111624247B - Reaction tank device for stabilizing biological nanopores for biological nano detection - Google Patents
Reaction tank device for stabilizing biological nanopores for biological nano detection Download PDFInfo
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- CN111624247B CN111624247B CN202010599877.2A CN202010599877A CN111624247B CN 111624247 B CN111624247 B CN 111624247B CN 202010599877 A CN202010599877 A CN 202010599877A CN 111624247 B CN111624247 B CN 111624247B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/333—Ion-selective electrodes or membranes
Abstract
The invention discloses a reaction tank device for stabilizing biological nanopores for biological nano detection, which comprises a device shell, a stabilizing device, a reaction tank, a clamping plate device, an upper sliding plate, a lower sliding plate, an electric cylinder, a rotating plate, a clamping steel wire, a rotating frame and a clamping plate. By arranging the stabilizing device, when the reaction tank receives external force and shakes, the stabilizing device can counteract the external force and stabilize the reaction tank, so that the inversion process is not influenced, and the working effect is improved.
Description
Technical Field
The invention belongs to the technical field of nano-channel detection, and particularly relates to a reaction tank device for stabilizing biological nanopores for biological nano-detection.
Background
The biological nanopore is constructed in a phospholipid bilayer by alpha-hemolysin to form an ion channel, and the movement of a single molecule can be accurately detected by detecting the current change at two sides of the channel. The bio-nanopore has extremely high sensitivity, but in the detection process, the structure of the phospholipid bilayer is too fragile and can be damaged when being subjected to external mechanical interference, such as vibration, movement and the like, so that the phospholipid bilayer cannot move before and after the structure is formed, the working efficiency is reduced, and the further development of the bio-nanopore reaction is limited, so that a device capable of stabilizing the structure in the reaction tank is needed.
Disclosure of Invention
Aiming at the technical problem, the invention provides a reaction tank device for stabilizing biological nanopores for biological nano detection, which prevents the detection effect from being influenced by severe shaking of the reaction tank through self-stabilization arranged at the lower end part, and improves the working efficiency.
In order to solve the above problems: the utility model provides a reaction tank device that is used for biological nanometer to detect stable biological nanopore, includes device shell, stabilising arrangement, reaction tank, splint device, upper and lower sliding plate, electric jar, rotor plate, buckle steel wire, rotating turret, buckle board, its characterized in that: the device shell include casing, anti-skidding callus on the sole, rotate the hinge support, the casing is the rectangle casing, inside cavity, anti-skidding callus on the sole set up the bottom at the casing, the rotation hinge support set up the position of leaning on in the inboard four corners of casing.
The stabilizing device comprises a reaction tank mounting groove, a spring hinge support, a buffer spring, a bottom hinge support, a spherical hinge rod, an upper moving rod, a lower moving rod, a telescopic spring, an upper clamping groove, a lower clamping groove, a bottom end plate, a spherical hinge electric cylinder and an upper end plate, wherein the reaction tank mounting groove is a rectangular shell, sliding grooves are arranged inside the left side and the right side of the reaction tank mounting groove, and the upper clamping groove and the lower clamping groove are arranged on the two sides of the sliding groove; the bottom hinged support is arranged on the left side and the right side of the lower end part of the reaction tank mounting groove, a telescopic rod is hinged to the bottom hinged support, the lower end part of the telescopic rod is hinged to the spring hinged support, a buffer spring is sleeved on the telescopic rod, and the lower end part of the spring hinged support is fixedly mounted with the lower bottom surface of the interior of the shell; the bottom end plate is fixedly arranged on the surface of the bottom surface of the inner side of the shell, the upper end plate is arranged above the bottom end plate, the spherical hinge electric cylinder is arranged between the bottom end plate and the upper end plate, and the upper end surface of the upper end plate is fixedly connected with the surface of the lower end part of the reaction tank mounting groove; the four corners of reaction tank mounting groove set up to the fillet, the intermediate position on every fillet limit is rectangular to be equipped with the round hole, upper and lower carriage release lever setting in the round hole, expanding spring is installed respectively at the upper and lower both ends of upper and lower carriage release lever, an end fixed mounting of expanding spring is downthehole at the reaction tank mounting groove, expanding spring's other end fixed mounting is provided with the ball pivot mount pad on the carriage release lever from top to bottom, the lower tip of ball pivot pole pass through the ball pivot mount pad and be connected with upper and lower carriage release lever, the upper end of ball pivot pole rotates and installs on rotating the hinge support.
The reaction tank is internally provided with two reaction tanks, and a clamping plate installation operation tank is arranged in the middle of the inner side of the reaction tank.
The clamp plate device comprises a clamp plate, a cylinder clamping groove, a circular reaction cylinder, a circular hole channel and a cylinder buckle, the clamp plate is a double-layer plate, a circular hole is formed in the clamp plate, the cylinder clamping groove is formed in the outer side of the circular hole, the circular reaction cylinder is sleeved in the circular hole, the circular hole channel is formed in the inner side of the circular reaction cylinder, the cylinder buckle is arranged on the outer edge of one side of the circular reaction cylinder, and the cylinder buckle is clamped in the cylinder clamping groove to form matching; the splint are slidably mounted in a splint mounting groove arranged on the reaction tank.
Furthermore, the rear end of the electric cylinder is hinged to the upper end of the reaction tank mounting groove, the rotating frame is fixedly arranged at the front end of the hinged support on the reaction tank mounting groove, the front end of the rotating plate is rotatably arranged on a rotating shaft arranged on the rotating frame, and the rear end of the rotating plate is hinged to the upper end of the electric cylinder.
Furthermore, the rear end part of the buckle steel wire is rotatably arranged in a round hole arranged in the middle of the rotating plate, and the buckle plates are fixedly arranged on two sides of the upper end surface of the clamping plate.
Furthermore, the anti-skidding foot pads are made of anti-skidding rubber materials, and the upper surfaces of the anti-skidding foot pads are attached to the mounting grooves formed in the bottom surface of the shell.
Furthermore, the upper end and the lower end of the spherical hinge electric cylinder are respectively connected with the upper end plate and the bottom end plate through spherical hinges, and every two spherical hinge electric cylinders are arranged in a V shape to form a parallel mechanism.
Furthermore, the upper and lower sliding plates comprise sliding plates, compression springs and compression rods, circular mounting grooves are formed in two sides of each sliding plate, each circular mounting groove is internally provided with a compression spring, the sliding plate at the rear end part of each compression spring is fixedly mounted, the compression springs are sleeved on the compression rods, and the front end parts of the compression springs are fixedly mounted with the compression rods; the sliding plates are fixedly arranged on two sides of the reaction tank, the sliding plates are slidably arranged in sliding grooves formed in the inner side of the reaction tank mounting groove, and the front end parts of the compression rods are clamped in the upper clamping groove and the lower clamping groove.
Furthermore, the front end part of the compression rod is a smooth spherical surface, the spherical surface is installed outwards, and the diameter of the spherical surface is equal to that of the round hole formed in the sliding plate.
Furthermore, a reaction tank cover is arranged at the top of the reaction tank and is connected with the reaction tank through a hinge.
Compared with the prior art, the invention has the beneficial effects that:
(1) by arranging the stabilizing device, when the reaction tank receives external force and shakes, the stabilizing device can counteract the external force and stabilize the reaction tank, so that the inversion process is not influenced, and the working effect is improved.
(2) The reaction channel can be flexibly replaced by arranging the clamping plate structure, so that the use engineering is facilitated, and the working efficiency is improved.
Drawings
Fig. 1 and 2 are schematic views of the overall structure of the present invention.
FIG. 3 is a schematic view of the internal structure of the present invention.
Fig. 4 is a cross-sectional view of the housing of the device of the present invention.
Fig. 5 and 7 are schematic structural views of the stabilizing device of the present invention.
Fig. 6 is a schematic structural diagram of the parallel mechanism of the present invention.
Fig. 8 is a schematic structural view of the splint device according to the present invention.
Fig. 9 is a schematic sectional view of the upper and lower sliding plates according to the present invention.
Fig. 10 is a schematic view of the buckle structure of the present invention.
Reference numerals: 1-a device housing; 2-a stabilizing device; 3-a reaction tank; 4-a splint device; 5-sliding plates up and down; 6-an electric cylinder; 7-a rotating plate; 8-buckling a steel wire; 9-a rotating frame; 10-a snap plate; 101-a housing; 102-anti-skid foot pads; 103-rotating hinged support; 201-reaction tank installation groove; 202-spring hinge support; 203-a buffer spring; 204-bottom hinged support; 205-spherical hinge rod; 206-up and down moving rod; 207-a telescopic spring; 208-upper and lower card slots; 209-bottom end plate; 210-a spherical hinge electric cylinder; 211-upper end plate; 401-splint; 402-cylinder card slot; 403-round reaction cylinder; 404-circular hole passage; 405-cylinder snap; 501-sliding plate; 502-compression spring; 503-compressing the rod.
Detailed Description
The present invention will be further described with reference to specific examples, which are illustrative of the invention and are not to be construed as limiting the invention.
Example (b): referring to fig. 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10, a reaction cell device for stabilizing biological nanopores for biological nanopores detection is disclosed, wherein a housing 101 is a rectangular housing, the interior of the housing is hollow, anti-slip foot pads 102 are installed at four corners of the bottom of the housing 101, the anti-slip foot pads 102 are made of anti-slip rubber materials, and the upper surfaces of the anti-slip foot pads 102 are attached to installation grooves formed in the bottom surface of the housing 101, so that the device is prevented from slipping when placed on the ground. The rotation hinge supports 103 are provided at upper positions of four corners of the inside of the housing 101, and two rotation hinge supports are provided at each corner.
The specific structure of the stabilizing device 2 is shown in fig. 5, wherein the reaction tank installation groove 201 is a rectangular shell, the volume of the reaction tank installation groove is smaller than that of the shell 101, the reaction tank installation groove is arranged in the shell 101, the bottom hinged supports 204 are arranged on the left and right sides of the lower end part of the reaction tank installation groove 201, each bottom hinged support 204 is hinged with a telescopic rod, the lower end part of each telescopic rod is hinged with the spring hinged support 202, the telescopic rods are sleeved with buffer springs 203, when the buffer springs 203 are compressed, the telescopic rods move together, and the lower end parts of the spring hinged supports 202 and the lower bottom surface in the shell 101 are fixedly installed through screws; sliding grooves are formed in the left side and the right side of the reaction tank installation groove 201, and upper clamping grooves and lower clamping grooves 208 are formed in the two sides of each sliding groove; the bottom end plate 209 is fixedly installed on the surface of the bottom surface of the inner side of the shell 101, an upper end plate 211 is arranged above the bottom end plate 209, a spherical hinge electric cylinder 210 is installed between the bottom end plate 209 and the upper end plate 211, the upper end surface of the upper end plate 211 is fixedly connected with the surface of the lower end part of the reaction tank installation groove 201, the upper end and the lower end of the spherical hinge electric cylinder 210 are respectively connected with the upper end plate 211 and the bottom end plate 209 through spherical hinges, and every two spherical hinge electric cylinders 210 are installed in a V shape to form a parallel mechanism. The three parallel mechanisms are respectively arranged at the bottom of the reaction tank mounting groove 201 to form a plane. The frame four corners of reaction tank mounting groove 201 sets up to the fillet, the intermediate position on every fillet limit is rectangular to be equipped with the round hole, upper and lower carriage release lever 206 slidable mounting is in the round hole, and expanding spring 207 is installed respectively at the upper and lower both ends of upper and lower carriage release lever 206, an end fixed mounting of expanding spring 207 is downthehole at reaction tank mounting groove 201, expanding spring 207's other end fixed mounting is on upper and lower carriage release lever 206, be provided with the ball pivot mount pad on the upper and lower carriage release lever 206, the lower tip of ball pivot lever 205 passes through the ball pivot mount pad and is connected with upper and lower carriage release lever 206, the upper end of ball pivot lever 205 rotates and installs on rotating hinged-support 103, when reaction unit appears rocking, stabilising arrangement can eliminate and rock, cushion.
In the preferred scheme of the invention, two reaction tanks are arranged in the reaction tank 3, and a splint installation operation tank is arranged in the middle of the inner side of the reaction tank 3 and is used for placing a splint device.
In a preferred scheme of the invention, a specific structure of the clamping plate device 4 is shown as 8, wherein the clamping plate 401 is a double-layer plate, the middle of the clamping plate is hollow, the upper end of the clamping plate is provided with a clamping plate 10, the clamping plate 401 is provided with four large circular through holes for accommodating a circular reaction cylinder 403, the outer side of each circular hole is provided with three cylindrical clamping grooves 402, the circular reaction cylinder 403 penetrates through one end of the circular hole and is sleeved in the circular hole, the outer edge of one side of the circular reaction cylinder 403 is provided with a cylindrical clamping buckle 405, and the cylindrical clamping buckle 405 is clamped in the cylindrical clamping grooves; the inboard of circular reaction cylinder 403 is provided with round hole passageway 404, for reaction channel splint 401, and splint device 4 slidable mounting is convenient for take out the operation such as change, clearance in the splint mounting groove that sets up on reaction tank 3 simultaneously.
At the both sides position on reaction tank mounting groove 201 top, be provided with the hinged-support, the rear end of electricity jar 6 sets up the hinged-support with reaction tank mounting groove 201 upper end and articulates, the front end position of hinged-support on reaction tank mounting groove 201 is fixed to dress, the preceding tip of rotor plate 7 rotates and installs in the axis of rotation that sets up on rotor plate 9, the rear end of rotor plate 7 is articulated with the upper end of electricity jar 6, the rear end rotation of buckle steel wire 8 is installed in the round hole that the intermediate position of rotor plate 7 set up, fixed mounting is in the both sides of the up end of splint 401. When the clamping plate device 4 needs to be fixed, the clamping steel wire is hung in the groove of the clamping plate 10, then the electric cylinder 6 contracts, and the clamping plate device 10 is fixed.
In a preferred embodiment of the present invention, the up-down sliding plate 5 includes a sliding plate 501, a compression spring 502, and a compression rod 503, wherein circular mounting grooves are formed on both sides of the sliding plate 501, the compression spring 502 is mounted in each circular mounting groove, the sliding plate 501 is fixedly mounted on the rear end portion of the compression spring 502, the compression spring 502 is sleeved on the compression rod 503, and the front end portion of the compression spring 502 is fixedly mounted on the compression rod 503; the sliding plates 501 are fixedly installed at both sides of the reaction tank 3, the sliding plates 501 are slidably installed in sliding grooves formed at the inner sides of the reaction tank installation grooves 201, and the front end portions of the compression rods 503 are clamped in the upper and lower clamping grooves 208. The front end of the compression rod 503 is a smooth spherical surface, the spherical surface is installed outwards, and the diameter of the spherical surface is equal to the diameter of the circular hole formed in the sliding plate 501. The purpose of this device is can stabilize the reaction tank and do not take place to slide in the spout, play stable effect.
The foregoing is only a preferred embodiment of this invention and it should be noted that modifications can be made by those skilled in the art without departing from the principle of the invention and these modifications should also be considered as the protection scope of the invention.
Claims (7)
1. The utility model provides a reaction tank device that is used for biological nanometer to detect stable biological nanopore, includes device shell (1), stabilising arrangement (2), reaction tank (3), splint device (4), upper and lower sliding plate (5), electric jar (6), rotor plate (7), buckle steel wire (8), rotating turret (9), buckle board (10), its characterized in that: the device shell (1) comprises a shell (101), an anti-skidding foot pad (102) and a rotating hinge support (103), wherein the shell (101) is a rectangular shell, the interior of the shell is hollow, the anti-skidding foot pad (102) is arranged at the bottom of the shell (101), and the rotating hinge support (103) is arranged at the upper positions of four corners of the inner side of the shell (101);
the stabilizing device (2) comprises a reaction tank mounting groove (201), a spring hinge support (202), a buffer spring (203), a bottom hinge support (204), a spherical hinge rod (205), an upper moving rod and a lower moving rod (206), a telescopic spring (207), upper and lower clamping grooves (208), a bottom end plate (209), a spherical hinge electric cylinder (210) and an upper end plate (211), wherein the reaction tank mounting groove (201) is a rectangular shell, sliding grooves are formed in the left side and the right side of the reaction tank mounting groove (201), and the upper and lower clamping grooves (208) are formed in the two sides of the sliding grooves; the bottom hinged support (204) is arranged on the left side and the right side of the lower end part of the reaction tank mounting groove (201), a telescopic rod is hinged to the bottom hinged support (204), the lower end part of the telescopic rod is hinged to the spring hinged support (202), a buffer spring (203) is sleeved on the telescopic rod, and the lower end part of the spring hinged support (202) is fixedly mounted with the lower bottom surface inside the shell (101); the bottom end plate (209) is fixedly arranged on the surface of the bottom surface of the inner side of the shell (101), an upper end plate (211) is arranged above the bottom end plate (209), a ball hinge electric cylinder (210) is arranged between the bottom end plate (209) and the upper end plate (211), and the upper end surface of the upper end plate (211) is fixedly connected with the surface of the lower end part of the reaction tank installation groove (201); four corners of the reaction tank mounting groove (201) are set to be round corners, a round hole is arranged in the middle position of each round corner edge in a long strip mode, the upper moving rod (206) and the lower moving rod (206) are arranged in the round holes, the upper end and the lower end of each upper moving rod (206) are respectively provided with a telescopic spring (207), one end of each telescopic spring (207) is fixedly arranged in each hole of the reaction tank mounting groove (201), the other end of each telescopic spring (207) is fixedly arranged on each upper moving rod (206), each upper moving rod (206) is provided with a spherical hinge mounting seat, the lower end of each spherical hinge rod (205) is connected with each upper moving rod (206) through the spherical hinge mounting seat, and the upper end of each spherical hinge rod (205) is rotatably arranged on the rotary hinge support (;
two reaction tanks are arranged in the reaction tank (3), and a splint mounting groove is arranged in the middle of the inner side of the reaction tank (3);
the clamp plate device (4) comprises a clamp plate (401), a cylinder clamping groove (402), a circular reaction cylinder (403), a circular hole channel (404) and a cylinder buckle (405), the clamp plate (401) is a double-layer plate, the clamp plate (401) is provided with a circular hole, the outer side of the circular hole is provided with the cylinder clamping groove (402), the circular reaction cylinder (403) is sleeved in the circular hole, the inner side of the circular reaction cylinder (403) is provided with the circular hole channel (404), the outer edge of one side of the circular reaction cylinder (40) is provided with the cylinder buckle (405), and the cylinder buckle (405) is clamped in the cylinder clamping groove (402) to form matching; the clamping plate (401) is arranged on the reaction tank (3) in a sliding way
And (6) loading in the groove.
2. The bio-nanopore stabilized reaction cell device for bio-nano detection according to claim 1, wherein: the rear end of electric jar (6) and reaction tank mounting groove (201) upper end set up the hinged-support articulated, rotating turret (9) fixed dress in the front position of hinged-support on reaction tank mounting groove (201), the preceding tip of rotor plate (7) rotate and install in the axis of rotation that sets up on rotating turret (9), the rear end of rotor plate (7) is articulated with the upper end of electric jar (6).
3. The bio-nanopore stabilized reaction cell device for bio-nano detection according to claim 1, wherein: the rear end part of the buckle steel wire (8) is rotatably arranged in a round hole arranged in the middle of the rotating plate (7), and the buckle plates (10) are fixedly arranged on two sides of the upper end surface of the clamping plate (401).
4. The bio-nanopore stabilized reaction cell device for bio-nano detection according to claim 1, wherein: the anti-skidding foot pads (102) are made of anti-skidding rubber materials, and the upper surfaces of the anti-skidding foot pads (102) are attached to mounting grooves formed in the bottom surface of the shell (101).
5. The bio-nanopore stabilized reaction cell device for bio-nano detection according to claim 1, wherein: the upper end and the lower end of each spherical hinge electric cylinder (210) are respectively connected with the upper end plate (211) and the bottom end plate (209) through spherical hinges, and each two spherical hinge electric cylinders (210) are arranged in a V shape to form a parallel mechanism.
6. The bio-nanopore stabilized reaction cell device for bio-nano detection according to claim 1, wherein: the upper and lower sliding plates (5) comprise sliding plates (501), compression springs (502) and compression rods (503), circular mounting grooves are formed in two sides of each sliding plate (501), each circular mounting groove is internally provided with a compression spring (502), and each compression spring (502)
The rear end sliding plate (501) is fixedly installed, a compression spring (502) is sleeved on a compression rod (503), and the front end part of the compression spring (502) is fixedly installed with the compression rod (503); the sliding plates (501) are fixedly arranged on two sides of the reaction tank (3), the sliding plates (501) are slidably arranged in sliding grooves formed in the inner sides of the reaction tank mounting grooves (201), and the front end parts of the compression rods (503) are clamped in the upper clamping grooves and the lower clamping grooves (208); the front end part of the compression rod (503) is a smooth spherical surface, the spherical surface is installed outwards, and the diameter of the spherical surface is equal to that of a round hole formed in the sliding plate (501).
7. The bio-nanopore stabilized reaction cell device for bio-nano detection according to claim 1, wherein: the top of the reaction tank (3) is provided with a reaction tank cover which is connected with the reaction tank (3) through a hinge.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010599877.2A CN111624247B (en) | 2020-06-28 | 2020-06-28 | Reaction tank device for stabilizing biological nanopores for biological nano detection |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010599877.2A CN111624247B (en) | 2020-06-28 | 2020-06-28 | Reaction tank device for stabilizing biological nanopores for biological nano detection |
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| CN111624247B true CN111624247B (en) | 2021-01-12 |
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| WO2013134610A1 (en) * | 2012-03-08 | 2013-09-12 | Quality Manufacturing Inc. | Touch sensitive robotic gripper |
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| EP0322178A2 (en) * | 1987-12-22 | 1989-06-28 | Beecham Group Plc | Cosmetic dispenser |
| CN201488972U (en) * | 2009-04-16 | 2010-05-26 | 福州大学 | Multi-channel gating device of electrochemical electrode array chip |
| CN104591548A (en) * | 2013-10-31 | 2015-05-06 | 登封市豫科玻璃技术有限公司 | Frosted glass reaction tank |
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