CN111592976A - Virus nucleic acid enrichment sandwich structure and gauze mask - Google Patents
Virus nucleic acid enrichment sandwich structure and gauze mask Download PDFInfo
- Publication number
- CN111592976A CN111592976A CN202010173768.4A CN202010173768A CN111592976A CN 111592976 A CN111592976 A CN 111592976A CN 202010173768 A CN202010173768 A CN 202010173768A CN 111592976 A CN111592976 A CN 111592976A
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- China
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- nucleic acid
- sandwich structure
- virus
- mask
- adsorption film
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D13/00—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches
- A41D13/05—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches protecting only a particular body part
- A41D13/11—Protective face masks, e.g. for surgical use, or for use in foul atmospheres
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D31/00—Materials specially adapted for outerwear
- A41D31/02—Layered materials
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D31/00—Materials specially adapted for outerwear
- A41D31/04—Materials specially adapted for outerwear characterised by special function or use
- A41D31/10—Impermeable to liquids, e.g. waterproof; Liquid-repellent
-
- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D31/00—Materials specially adapted for outerwear
- A41D31/04—Materials specially adapted for outerwear characterised by special function or use
- A41D31/12—Hygroscopic; Water retaining
Abstract
The invention relates to the technical field of virus detection, in particular to a virus nucleic acid enrichment sandwich structure and a mask. The sandwich structure can be used for enriching virus nucleic acid, can be used as a common mask after being applied to the mask, and can also be used for enriching virus nucleic acid, and the masks on the market can only block dust, bacteria, viruses and the like. After the mask is used, the nucleic acid enriched in the mask is directly detected, so that the operation can be simplified, and the probability of infection of medical personnel is reduced.
Description
Technical Field
The invention relates to the technical field of virus detection, in particular to a virus nucleic acid enrichment sandwich structure and a mask.
Technical Field
The virus particles are very small, typically below 0.3 microns in diameter. The masks currently used, whether medical masks (represented by various surgical masks of 3M company in usa) or common civil masks, are made of textile. The mask is commonly used in hospitals and daily lives because the liquid spray in the oral cavity and the nasal cavity can be blocked by wearing the mask, and the bacteria or viruses can be brought into the human body by preventing the dirty hands from accidentally touching the mouth and the nose.
Pharyngeal swabs are currently the most common way of sampling in the oral and nasal cavities. The sampling of throat swab is the most convenient to popularize, and maneuverability is strong, and the sputum does not all have at any time, and the extraction of alveolar lavage fluid relates to the bronchoscope, and the operation is inconvenient, and medical personnel infects more easily moreover. If a virus nucleic acid enrichment mask is used, the nucleic acid enriched by the mask is directly detected, the operation can be simplified, and the infection probability of medical staff is reduced.
Disclosure of Invention
The invention aims to provide a virus nucleic acid enrichment sandwich structure and a mask, wherein the sandwich structure can be used for enriching virus nucleic acid, and not only can be used as a common mask after being applied to the mask, but also can be used for directly detecting nucleic acid enriched in the mask, so that the operation can be simplified, and the infection probability of medical care personnel can be reduced.
The invention relates to a virus nucleic acid enrichment sandwich structure, which is characterized in that: the sandwich structure is rectangular and comprises an activated carbon fiber cloth A and a virus adsorption film A from outside to inside, the edges of the peripheries of the activated carbon fiber cloth A and the nitrocellulose film A are sewn together, and a plurality of vent holes are uniformly distributed on the surface of the virus adsorption film A.
Wherein, the preferred scheme is as follows:
the sandwich structure can also be a four-layer structure, so that the capture probability of the virus is increased. The virus adsorption film comprises a virus adsorption film A and a virus adsorption film B, wherein the inner side surface of the virus adsorption film A further comprises an activated carbon fiber cloth B and the virus adsorption film B from outside to inside, the peripheral edges of the activated carbon fiber cloth A, the virus adsorption film A, the activated carbon fiber cloth B and the virus adsorption film B are sewn together, and a plurality of vent holes are uniformly distributed on the surface of the virus adsorption film B. In the invention, the activated carbon fiber cloth A and the activated carbon fiber cloth B can absorb water vapor and are used for soaking the virus adsorption film A and the virus adsorption film B so as to play a role of enriching virus nucleic acid. The virus adsorption film A and the virus adsorption film B are provided with vent holes for facilitating the air flow to pass through.
The virus adsorption film A and the virus adsorption film B are soaked in a trace amount of virus lysate for 0.5-2 hours in advance, and then are dried for use. The used micro-amount of virus lysate can crack viruses to release nucleic acid, and can protect the virus nucleic acid from degradation.
The virus adsorption membrane A and the virus adsorption membrane B are nitrocellulose membranes or nylon membranes.
The micro-scale virus lysate is DEPC water added with 100mM tris, 10mM EDTA, 0.5M KCl and 0.05% sodium dodecyl sarcosinate.
The invention relates to a virus nucleic acid enrichment mask, which comprises a mask surface body and tightening belts on two sides of the mask surface body, wherein the mask surface body sequentially comprises a moisture absorption layer, a filtering layer and a water blocking layer from outside to inside, and is characterized in that: an interlayer structure is arranged between the moisture absorption layer and the filter layer, and the interlayer structure is positioned at the position of the mask body, which is just opposite to the mouth and the nose.
The length of the sandwich structure is 3-10 cm, and the width of the sandwich structure is 2-6 cm.
The moisture absorption layer is provided with an opening which can be opened and closed and is used for taking and placing the sandwich structure. The opening can adopt structural design such as removable stitch, magic subsides, button, and this patent is not restricted to this, as long as can play with sandwich structure put into between moisture absorption layer and the filter layer can.
The moisture absorption layer is non-woven fabric or melt-blown fabric, the filter layer is melt-blown fabric, and the water-resistant layer is sanitary gauze or non-woven fabric.
Compared with the prior art, the sandwich structure can be used for enriching virus nucleic acid, can be used as a common mask after being applied to the mask, and can also be used for enriching virus nucleic acid, and the masks in the market can only block dust, bacteria, viruses and the like. After the mask is used, the nucleic acid enriched in the mask is directly detected, so that the operation can be simplified, and the probability of infection of medical personnel is reduced.
Drawings
FIG. 1 is a schematic view of a mask according to the present invention;
FIG. 2 is a schematic view of a virus-adsorbing membrane A according to the present invention;
FIG. 3 is an exploded view of the sandwich structure of the present invention;
in the drawings are labeled: 1-mask surface body, 2-sandwich structure, 3-activated carbon fiber cloth A, 4-virus adsorption film A, 5-activated carbon fiber cloth B, 6-virus adsorption film B.
Detailed Description
The invention is further illustrated by the following figures and examples, which are not to be construed as limiting the invention.
Example 1:
as shown in fig. 2 to 3, a viral nucleic acid enrichment sandwich structure is characterized in that: the sandwich structure 2 is a rectangular four-layer structure and comprises an activated carbon fiber cloth A3, a virus adsorption film A4, an activated carbon fiber cloth B5 and a virus adsorption film B6 from outside to inside, the peripheral edges of the activated carbon fiber cloth A3, the virus adsorption film A4, the activated carbon fiber cloth B5 and the virus adsorption film B6 are sewn together, and a plurality of vent holes are uniformly distributed on the surfaces of the virus adsorption film A4 and the virus adsorption film B6. In this embodiment, the activated carbon fiber cloth A3 and the activated carbon fiber cloth B5 can absorb water vapor, and are used to soak the virus adsorption film a4 and the virus adsorption film B6, so that they can enrich viral nucleic acids. The virus-adsorbing film A4 and the virus-adsorbing film B6 have air vents for facilitating the air flow.
The virus adsorption film A4 and the virus adsorption film B6 are soaked in trace virus lysate for 1 hour in advance, and then are dried for use. The used micro-amount of virus lysate can crack viruses to release nucleic acid, and can protect the virus nucleic acid from degradation.
The virus adsorption film A4 and the virus adsorption film B6 are nitrocellulose films.
The micro-scale virus lysate is DEPC water added with 100mM tris, 10mM EDTA, 0.5M KCl and 0.05% sodium dodecyl sarcosinate.
Example 2:
as shown in fig. 1, the mask for virus nucleic acid enrichment comprises a mask body 1 and tensioning belts arranged on two sides of the mask body 1, wherein the mask body 1 sequentially comprises a moisture absorption layer, a filter layer and a water-resistant layer from outside to inside, an interlayer structure 2 in the embodiment 1 is arranged between the moisture absorption layer and the filter layer, and the interlayer structure 2 is positioned at a position, right opposite to the mouth and nose, of the mask body 1.
The sandwich structure 2 has a length of 5cm and a width of 3 cm.
The moisture-absorbing layer has an opening which can be opened and closed for access to the sandwich structure 2. The opening can adopt structural design such as removable stitch, magic subsides, button, and this patent is not restricted to this, as long as can play with sandwich structure 2 put into between moisture absorption layer and the filter layer can.
The mask body 1 can be directly applied to a conventional medical mask, for example, the moisture absorption layer is a non-woven fabric, the filter layer is melt-blown fabric, and the water-resistant layer is a non-woven fabric.
After the virus nucleic acid enrichment mask is used, the sandwich structure 2 is taken out and cut open, and the nitrocellulose membrane is taken out. And (3) shearing a square with the side length of 4cm at the middle part of the nitrocellulose membrane by using a pair of scissors, shearing the square into pieces to be used as a sample, extracting nucleic acid by using a standard nucleic acid extraction method, and carrying out a subsequent nucleic acid detection process. The mask can enrich nucleic acid and detect, simplify sampling operation of medical personnel, and reduce infection probability of medical personnel.
Claims (9)
1. A viral nucleic acid enrichment sandwich structure, characterized in that: the sandwich structure is rectangular and comprises an activated carbon fiber cloth A and a virus adsorption film A from outside to inside, the edges of the peripheries of the activated carbon fiber cloth A and the nitrocellulose film A are sewn together, and a plurality of vent holes are uniformly distributed on the surface of the virus adsorption film A.
2. The viral nucleic acid enrichment sandwich structure of claim 1, wherein: the virus adsorption film A is characterized by further comprising an activated carbon fiber cloth B and a virus adsorption film B from outside to inside, the edges of the periphery of the activated carbon fiber cloth A, the virus adsorption film A, the activated carbon fiber cloth B and the virus adsorption film B are sewn together, and a plurality of vent holes are uniformly distributed on the surface of the virus adsorption film B.
3. A viral nucleic acid enrichment sandwich structure according to claim 2, wherein: the virus adsorption film A and the virus adsorption film B are soaked in a trace amount of virus lysate for 0.5-2 hours in advance, and then are dried for use.
4. A viral nucleic acid enrichment sandwich structure according to claim 3, wherein: the virus adsorption membrane A and the virus adsorption membrane B are nitrocellulose membranes or nylon membranes.
5. A viral nucleic acid enrichment sandwich structure according to claim 3, wherein: the micro virus lysate is DEPC water added with 100mM tris, 10mM EDTA, 0.5 mM KCl and 0.05% of sodium dodecyl sarcosinate.
6. The utility model provides a virus nucleic acid enrichment gauze mask, includes the taut area of gauze mask face body and gauze mask face body both sides, gauze mask face body outside-in includes moisture absorption layer, filter layer and water blocking layer, its characterized in that in proper order: an interlayer structure is arranged between the moisture absorption layer and the filter layer, and the interlayer structure is positioned at the position of the mask body, which is just opposite to the mouth and the nose.
7. The mask for enriching viral nucleic acid according to claim 6, wherein: the length of the sandwich structure is 3-10 cm, and the width of the sandwich structure is 2-6 cm.
8. The mask for enriching viral nucleic acid according to claim 6, wherein: the moisture absorption layer is provided with an opening which can be opened and closed and is used for taking and placing the sandwich structure. The opening can adopt structural design such as removable stitch, magic subsides, button, and this patent is not restricted to this, as long as can play with sandwich structure put into between moisture absorption layer and the filter layer can.
9. The mask for enriching viral nucleic acid according to claim 6, wherein: the moisture absorption layer is non-woven fabric or melt-blown fabric, the filter layer is melt-blown fabric, and the water-resistant layer is sanitary gauze or non-woven fabric.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010173768.4A CN111592976B (en) | 2020-03-13 | 2020-03-13 | Virus nucleic acid enrichment sandwich structure and gauze mask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010173768.4A CN111592976B (en) | 2020-03-13 | 2020-03-13 | Virus nucleic acid enrichment sandwich structure and gauze mask |
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| Publication Number | Publication Date |
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| CN111592976A true CN111592976A (en) | 2020-08-28 |
| CN111592976B CN111592976B (en) | 2021-09-03 |
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| CN202010173768.4A Active CN111592976B (en) | 2020-03-13 | 2020-03-13 | Virus nucleic acid enrichment sandwich structure and gauze mask |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022073479A1 (en) * | 2020-10-06 | 2022-04-14 | 刘静怡 | Face mask used to collect human/animal respiratory virus sample |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203302390U (en) * | 2013-06-07 | 2013-11-27 | 上海舒爽材料科技有限公司 | Dustproof and antibacterial mask filter sheet |
| CN107164493A (en) * | 2017-06-08 | 2017-09-15 | 杭州遂真生物技术有限公司 | A kind of GBS kit for detecting nucleic acid |
| KR101948439B1 (en) * | 2017-10-11 | 2019-02-14 | 건국대학교 산학협력단 | Ozone mask |
| CN208972707U (en) * | 2018-09-30 | 2019-06-14 | 云南炳泰科技有限公司 | A kind of surgical face mask |
| JP2019199668A (en) * | 2018-05-17 | 2019-11-21 | 帝人フロンティア株式会社 | Mask filter and face mask |
-
2020
- 2020-03-13 CN CN202010173768.4A patent/CN111592976B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203302390U (en) * | 2013-06-07 | 2013-11-27 | 上海舒爽材料科技有限公司 | Dustproof and antibacterial mask filter sheet |
| CN107164493A (en) * | 2017-06-08 | 2017-09-15 | 杭州遂真生物技术有限公司 | A kind of GBS kit for detecting nucleic acid |
| KR101948439B1 (en) * | 2017-10-11 | 2019-02-14 | 건국대학교 산학협력단 | Ozone mask |
| JP2019199668A (en) * | 2018-05-17 | 2019-11-21 | 帝人フロンティア株式会社 | Mask filter and face mask |
| CN208972707U (en) * | 2018-09-30 | 2019-06-14 | 云南炳泰科技有限公司 | A kind of surgical face mask |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022073479A1 (en) * | 2020-10-06 | 2022-04-14 | 刘静怡 | Face mask used to collect human/animal respiratory virus sample |
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| CN111592976B (en) | 2021-09-03 |
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Address after: 266000 Room 201, North 1 / F, Haike R & D center, 76 Menghai 2nd Road, aoshanwei sub district office, Jimo District, Qingdao City, Shandong Province Applicant after: Qingdao DiNova Gene Technology Co.,Ltd. Applicant after: SHANDONG YISHENG LIVESTOCK & POULTRY BREEDING Co.,Ltd. Applicant after: Shijiazhuang Shengbo Biotechnology Co.,Ltd. Applicant after: Hebei lvwo Biotechnology Co.,Ltd. Applicant after: QINGDAO INSIGHT EXBIO Co.,Ltd. Address before: 266000 room 306, building D20, Qingdao doctor Pioneer Park, 89 Changcheng Road, Chengyang street, Chengyang District, Qingdao City, Shandong Province Applicant before: Qingdao DiNova Gene Technology Co.,Ltd. Applicant before: SHANDONG YISHENG LIVESTOCK & POULTRY BREEDING Co.,Ltd. Applicant before: Shijiazhuang Shengbo Biotechnology Co.,Ltd. Applicant before: Hebei lvwo Biotechnology Co.,Ltd. Applicant before: QINGDAO INSIGHT EXBIO Co.,Ltd. |
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