CN111557949B - Medicine or health product containing hydrogen-rich water and/or NMN and preparation method thereof - Google Patents
Medicine or health product containing hydrogen-rich water and/or NMN and preparation method thereof Download PDFInfo
- Publication number
- CN111557949B CN111557949B CN202010472676.6A CN202010472676A CN111557949B CN 111557949 B CN111557949 B CN 111557949B CN 202010472676 A CN202010472676 A CN 202010472676A CN 111557949 B CN111557949 B CN 111557949B
- Authority
- CN
- China
- Prior art keywords
- hydrogen
- nmn
- rich water
- intestinal
- combination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229910052739 hydrogen Inorganic materials 0.000 title claims abstract description 98
- 239000001257 hydrogen Substances 0.000 title claims abstract description 98
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 title claims abstract description 94
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 89
- 239000003814 drug Substances 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 230000036541 health Effects 0.000 title description 11
- 229940079593 drug Drugs 0.000 title description 4
- 230000000968 intestinal effect Effects 0.000 claims abstract description 46
- 239000007789 gas Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 11
- 150000002431 hydrogen Chemical class 0.000 claims description 5
- 239000002417 nutraceutical Substances 0.000 claims description 5
- 235000021436 nutraceutical agent Nutrition 0.000 claims description 5
- 230000033116 oxidation-reduction process Effects 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 3
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 238000007911 parenteral administration Methods 0.000 claims 1
- DAYLJWODMCOQEW-TURQNECASA-O NMN(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(O)=O)O2)O)=C1 DAYLJWODMCOQEW-TURQNECASA-O 0.000 description 84
- 210000001035 gastrointestinal tract Anatomy 0.000 description 30
- 241000699666 Mus <mouse, genus> Species 0.000 description 26
- 210000004027 cell Anatomy 0.000 description 24
- 241000699670 Mus sp. Species 0.000 description 21
- 239000000047 product Substances 0.000 description 13
- 241000186000 Bifidobacterium Species 0.000 description 12
- 241000282414 Homo sapiens Species 0.000 description 12
- 241000186660 Lactobacillus Species 0.000 description 12
- 230000003247 decreasing effect Effects 0.000 description 12
- 230000006872 improvement Effects 0.000 description 12
- 108060003951 Immunoglobulin Proteins 0.000 description 10
- 229940000425 combination drug Drugs 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 102000018358 immunoglobulin Human genes 0.000 description 10
- 210000004347 intestinal mucosa Anatomy 0.000 description 10
- 241000305071 Enterobacterales Species 0.000 description 9
- 239000012895 dilution Substances 0.000 description 9
- 238000010790 dilution Methods 0.000 description 9
- 101150063569 slgA gene Proteins 0.000 description 8
- 241000194033 Enterococcus Species 0.000 description 7
- 229940039696 lactobacillus Drugs 0.000 description 7
- 239000000725 suspension Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 241000588914 Enterobacter Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 206010006326 Breath odour Diseases 0.000 description 3
- 206010010774 Constipation Diseases 0.000 description 3
- 208000032139 Halitosis Diseases 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 3
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 3
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 3
- 241001052560 Thallis Species 0.000 description 3
- 230000003110 anti-inflammatory effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 210000003405 ileum Anatomy 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 206010022437 insomnia Diseases 0.000 description 3
- 208000030159 metabolic disease Diseases 0.000 description 3
- 238000010172 mouse model Methods 0.000 description 3
- 208000015122 neurodegenerative disease Diseases 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000003248 secreting effect Effects 0.000 description 3
- 229910052711 selenium Inorganic materials 0.000 description 3
- 239000011669 selenium Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000252983 Caecum Species 0.000 description 2
- 238000012449 Kunming mouse Methods 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 102100033223 Nicotinamide phosphoribosyltransferase Human genes 0.000 description 2
- 108010064862 Nicotinamide phosphoribosyltransferase Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 210000004534 cecum Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000008297 liquid dosage form Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 210000004877 mucosa Anatomy 0.000 description 2
- 230000004770 neurodegeneration Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 239000012898 sample dilution Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000003964 Histone deacetylase Human genes 0.000 description 1
- 108090000353 Histone deacetylase Proteins 0.000 description 1
- 206010022714 Intestinal ulcer Diseases 0.000 description 1
- 208000005102 Kashin-Beck Disease Diseases 0.000 description 1
- 208000019926 Keshan disease Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010063837 Reperfusion injury Diseases 0.000 description 1
- 102000011990 Sirtuin Human genes 0.000 description 1
- 108050002485 Sirtuin Proteins 0.000 description 1
- 102000000344 Sirtuin 1 Human genes 0.000 description 1
- 108010041191 Sirtuin 1 Proteins 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- -1 i.e. Substances 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 208000012947 ischemia reperfusion injury Diseases 0.000 description 1
- 238000010150 least significant difference test Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/13—Nucleic acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/36—Hydrogen production from non-carbon containing sources, e.g. by water electrolysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a medicament or health-care product containing hydrogen-rich water and/or NMN and a preparation method thereof. According to the technical scheme, the intestinal flora is improved by hydrogen-rich water and/or NMN.
Description
Technical Field
The invention relates to the technical field of food and medicine, in particular to a medicine or health-care product containing hydrogen-rich water and/or NMN and a preparation method thereof.
Background
The intestinal tract is an important nutrient absorption and immune organ of a human body, 60% -70% of immune cells of the human body are concentrated in the intestinal tract, the intestinal tract is a main battlefield of body immunity, the intestinal tract health is closely related to intestinal tract flora, the intestinal tract flora and the human body are interdependent and symbiotic to form an intestinal tract microecological system, the intestinal tract microecological system participates in body metabolism, energy balance, immune regulation and the like, the change of the intestinal tract microecological system is related to the occurrence of various diseases, such as constipation, insomnia, halitosis and the like, and the long-term accumulation of toxins in the body can even cause cancer to endanger life. Therefore, the balance of the intestinal flora is very important for keeping the intestinal health, can be used as a wind vane for human health, and has profound significance for health by improving the condition of the intestinal flora.
At present, intestinal tract improvement is mostly carried out through living microorganisms such as probiotics and the like, the intestinal tract improvement can play a role in benefiting health of a host, but the intestinal tract improvement has the related problems that thalli are easy to die, the requirement on the storage environment is met and the like, so that certain economic cost is needed for popularization. The method for improving the intestinal environment by compounding the selenium element and the xylo-oligosaccharide is available, but the selenium source is inorganic selenium which is mostly used for drug treatment of patients under extreme conditions, such as keshan disease, Kashin-Beck disease, cancer and the like, and has the advantages of great toxic and side effects, low absorption rate and unobvious effect on improvement of the intestinal environment.
Disclosure of Invention
The invention mainly aims to provide a medicament or health-care product containing hydrogen-rich water and/or NMN and a preparation method thereof, aiming at improving the intestinal flora of organisms.
In order to achieve the purpose, the invention provides an application of a combined medicine or health-care product containing hydrogen-rich water and NMN in preparing a medicine for improving intestinal flora, wherein the hydrogen content of saturated nanoscale soluble hydrogen molecular groups in the hydrogen-rich water is 0.50-2.0 mg/L; and/or the presence of a gas in the gas,
the pH range of the hydrogen-rich water is 5.85-6.15; and/or the presence of a gas in the gas,
the oxidation-reduction potential of the hydrogen-rich water is-100 to-150 mV;
the combined medicine is characterized in that the mass of NMN dissolved in each 1mL of the hydrogen-rich water is 0.3-2.5 g.
In one embodiment, the single dose of the combination drug is 0.35-1.5 mg/mL/day.
In one embodiment, the combination drug or health product is in a liquid dosage form.
In one embodiment, the combined medicine or health product is in a gastrointestinal administration dosage form.
In one embodiment, the method for preparing the pharmaceutical composition or health product containing hydrogen-rich water and NMN comprises: and dissolving the NMN in the hydrogen-rich water, and uniformly mixing to obtain the combined medicine of the hydrogen-rich water and the NMN.
Compared with the prior art, the invention has the following beneficial effects:
the technical scheme of the invention discloses application of a medicine or health-care product containing hydrogen-rich water and NMN in improving intestinal flora, and the medicine or health-care product containing hydrogen-rich water and NMN can effectively improve the intestinal flora by taking, thereby improving the balance of intestinal microecological systems and reducing various diseases caused by the intestinal flora, such as constipation, insomnia, halitosis and the like. Can be taken for a long time without side effect.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the related art, hydrogen-rich water, i.e., water containing hydrogen rich gas, is used. The hydrogen-rich water can effectively prevent and treat various diseases by reducing oxidation reaction and inflammation reaction, such as protection of ischemia reperfusion injury, protection of organ transplantation, treatment of metabolic diseases, treatment of neurodegenerative diseases, treatment of intestinal ulcer inflammation and the like. NMN (nicotinamide mononucleotide) is the product of the nicotinamide phosphoribosyltransferase reaction, one of the key prerequisites for NAD +. In mammals, NMN is produced by nicotinamide under catalysis of Nampt, followed by NMN producing NAD + under catalysis of nicotinamide mononucleotide adenyl transferase. NMN exerts its physiological functions in the human body by conversion to NAD +, such as activating NAD + substrate-dependent Sirt1 (histone deacetylase, also known as sirtuin), regulating cell survival and death, maintaining redox status, and the like. Recent research shows that the NMN in the organism is regulated to have better treatment and repair effects on cardiovascular and cerebrovascular diseases, neurodegenerative diseases, aging degenerative diseases and the like; in addition, NMN has reducing and anti-inflammatory capabilities.
Based on this, the invention provides a medicament or health product containing hydrogen-rich water and/or NMN, which is used for improving the intestinal flora.
The hydrogen-rich water contains nano-scale soluble hydrogen molecular groups, and the hydrogen molecules can participate in signal conduction of different flora in intestinal tract, so that the hydrogen-rich water has anti-inflammatory and anti-oxidation effects, has a certain protection effect on intestinal mucosa, and can promote intestinal tract peristalsis and regulate intestinal tract digestive dysfunction. According to the technical scheme, the improvement on the intestinal flora is realized by the hydrogen-rich water with different administration doses.
NMN (nicotinamide mononucleotide) can be converted into NAD in cells, so that Sirt protein 1-7 subtypes are activated, and the NMN has the effects of resisting aging, oxidation and inflammation, protecting intestinal mucosa cells and improving the intestinal environment. In the technical scheme of the invention, the improvement of intestinal flora is realized by NMN with different administration doses.
Furthermore, the technical scheme of the invention also considers that NMN (nicotinamide mononucleotide) can provide prebiotics for intestinal flora, and when the NMN is compatible with hydrogen-rich water for use, a synergistic anti-inflammatory effect is generated. On one hand, the intestinal mucosa cell protection, the intestinal microenvironment improvement and the growth promotion of probiotics can be improved; on the other hand, the intestinal flora can be regulated and improved by inhibiting the predominant growth of inflammatory intestinal bacteria, so that the occurrence of diseases such as constipation, insomnia, halitosis and the like caused by the imbalance of the intestinal flora is reduced. Therefore, the technical scheme of the invention also aims to realize the improvement of the intestinal flora through the combination drug of the hydrogen-rich water and the NMN.
Optionally, the hydrogen content of the saturated nanoscale soluble hydrogen molecular groups in the selected hydrogen-rich water is 0.50-2.0 mg/L; and/or the presence of a gas in the gas,
the pH range of the hydrogen-rich water is 5.85-6.15; and/or the presence of a gas in the gas,
the oxidation-reduction potential of the hydrogen-rich water is-100 to-150 mV.
Optionally, when the combination of the hydrogen-rich water and the NMN is used, the weight of the combination to dissolve the NMN per 1mL of the hydrogen-rich water is 0.3-2.5 g.
Optionally, when the combination drug is used, the single dose concentration of the combination drug is 0.35-1.5 mg/mL/day.
Optionally, when the hydrogen-rich water is used alone, the single dose of the hydrogen-rich water is. 5-25 ml/Kg/day.
Optionally, the single dose amount of NMN is when the NMN is used alone. 0.3-1.8 mg/Kg/day.
Optionally, the pharmaceutical or nutraceutical is in liquid form.
In the embodiment, compared with the administration modes of inhalation and intravenous injection, the administration mode of drinking is safer and more convenient, has higher practicability and popularity, and is suitable for long-term administration. In this regard, this example provides a pharmaceutical or nutraceutical formulation containing hydrogen-rich water and/or NMN in liquid dosage form.
Specifically, the medicine or the health care product is a gastrointestinal administration dosage form.
The invention also provides a preparation method of the medicine or health-care product containing the hydrogen-rich water and/or NMN. Specifically, when the combined medicine of the hydrogen-rich water and the NMN is used, the NMN is dissolved in the hydrogen-rich water and uniformly mixed to obtain the combined medicine of the hydrogen-rich water and the NMN.
To further illustrate the improvement effect of hydrogen-rich water and/or NMN on intestinal flora in the technical solution of the present invention, the following examples are selected for specific illustration. It is emphasized that the set of embodiments described below is merely a subset of embodiments of the invention and not all embodiments. (the hydrogen-rich water selected in the following example group is supersaturated nanoscale soluble hydrogen clusters, hydrogen content of 0.95mg/L, pH 5.95, oxidation reduction potential-126.75 mV. NMN selected in the following example group was purchased from Shanghai Pifu Biotech Ltd.)
Example set 1
HFA mouse model construction
1. Obtaining flora: recruiting a healthy volunteer (female, 25 years old, without digestive tract and metabolic diseases, and without taking antibiotics within 90 days), taking the feces discharged by the volunteer for the first time in the morning, aseptically collecting, weighing within 1 hour under aseptic condition, adding Wikins-Chalgren anaerobic bacteria broth (Oxoid) according to the mass ratio of 1:9 (m: V) for dilution, and oscillating and mixing to obtain suspension, namely suspension containing thalli.
2. HFA mouse model: a plurality of sterile KM mice are prepared, the KM mice are 4 weeks old, 18-22 g in weight and half of male and female mice respectively. Taking 0.3mL of the suspension containing the thalli in the step 1 per mouse, and performing intragastric lavage on each mouse so as to transfer the flora into the mouse; the culture is carried out for 21 days, so that the intestinal flora of the human is colonized in the intestinal tract of the mouse, and an HFA mouse model is obtained.
Example group 2
In vivo experiment of hydrogen-rich water on improving intestinal flora
1. Breeding the HFA mice: 40 HFA mice were randomly assigned to 4 groups of 10 mice each, a normal control group. Performing intragastric administration on each mouse according to the dosage of the hydrogen-rich water shown in the table 1, performing intragastric administration 6 times every day for 30 days continuously, and detecting the intestinal flora content and the intestinal mucosa antibody content of the mouse after 30 days. (free drinking water for mouse in feeding process)
TABLE 1 dosing of hydrogen-rich water for intragastric administration in each example group
2. Detecting the content of each thallus of the intestinal flora of the mice: fixing the mice which are raised for 30 days by using hydrogen-rich water, disinfecting the skin, cutting the abdomen, and aseptically collecting cecal contents; placing the contents in a sterile test tube for later use; weighing 1.0g of caecum content in a super clean bench, adding 9mL of sterilized normal saline, mixing and oscillating for 15min to obtain suspension of the caecum content; taking 6 test tubes filled with 9mL of sterilized normal saline, and sequentially diluting the cecal contents according to the concentration gradient until the dilution multiple is 10 -5 . Specifically, the sample dilution gradient was bifidobacterium (10) -3 、10 -4 、10 -5 ) Lactobacillus (10) -3 、10 -4 、10 -5 ) Enterobacter (10) -3 、10 -4 、10 -5 ) And enterococcus (10) -3 、10 -4 、10 -5 ). Then, 0.2mL of samples with different dilution concentrations were pipetted by pipette onto different selection media, and spread for culture, and 3 sets of parallel experiments were performed at each dilution concentration. The culture conditions and identification methods of different intestinal flora are shown in table 2.
TABLE 2 method for culturing and identifying intestinal flora of mice
After the end of the cell culture, the number of viable bacteria was counted by a conventional plate counting method, and the result data shown in Table 3 was obtained.
It is emphasized that all data results were statistically analyzed using the SPSS2.0 software, with the results expressed as mean, significance analysis using the one-way ANOVA, and multiple comparisons between groups using the LSD test, with P < 0.05 as the difference being statistically significant.
The formula for plate cell count is shown below: (preferably, a plate culture dish with 30-300 bacteria colonies is selected during counting)
Wherein alpha is the average colony number of each colony under the same dilution, and each dilution comprises 3 groups of parallel tests;
the total number of cells is expressed as the log of cells per gram of feces.
TABLE 3 bacterial flora and number of cells in the intestinal tract of HFA mice (P < 0.05, P < 0.01, compared to blank)
As can be seen from table 3 above, at a given hydrogen-rich water dose, the number of cells of bifidobacterium and lactobacillus increases with the increase of the hydrogen-rich water dose, and the number of cells of enterobacter and enterococcus decreases with the increase of the hydrogen-rich water dose; after a certain amount of hydrogen-rich water is added, the number of the enterobacteria and enterococci increases with the increase of the amount of hydrogen-rich water. Wherein, when the hydrogen-rich water dose reaches 9 mL/day, the intestinal bifidobacteria of the mice are increased from 8.627 + -0.521 to 9.086 + -0.461, the lactobacillus is increased from 8.551 + -0.031 to 9.067 + -0.033, the enterobacteria are decreased from 8.667 + -0.046 to 7.091 + -0.095, and the enterococcus is decreased from 7.998 + -0.042 to 7.719 + -0.044. It is demonstrated that hydrogen rich water can achieve an improvement in intestinal flora.
3. Detection of mouse intestinal mucosa antibody content: taking the ileum 2 cm at the tail end of the intestinal tract of the mouse, placing the ileum in a sterile beaker, washing the cavity of the ileum by using a phosphate buffer solution in a sterile ice bath until all excrement is removed, taking 1g of 5mL of PBS buffer solution of the ileal mucosa, homogenizing the solution on ice by using a 10mL glass homogenizer, centrifuging the solution for 10min at 1000r/min at 4 ℃, and collecting the supernatant to be placed at-20 ℃ for later use. The content of the secretory immunoglobulin in the ileal mucosa is determined according to the instructions of the slg A kit, and the specific operation is not repeated here. The data results shown in table 4 were obtained.
Table 4 HFA mouse intestinal mucosa slg a content (P < 0.05, P < 0.01 compared to blank)
As can be seen from table 4 above, at a given hydrogen-rich water dose, the cell concentration in the intestinal tract of the mouse increased with the increase of the hydrogen-rich water dose, and the content of secretory immunoglobulin (slgA) in the intestinal tract of the mouse increased with the increase of the hydrogen-rich water dose. Wherein the OD is the maximum cell concentration reached at a hydrogen-rich water dose of 18 mL/day 450 The value reached a maximum of 0.1019 ± 0.0038; also, the maximum secreted immunoglobulin (slgA) content was reached, which was 5.199. + -. 0.0054. multidot.pg/mL.
Example set 3
In vivo experiment of NMN for improving intestinal flora
1. HFA mice were housed: 40 HFA mice were randomly assigned to 4 groups of 10 mice each, a normal control group. And (3) performing intragastric gavage on each mouse according to the NMN dose shown in the table 5, performing intragastric gavage 6 times every day for 30 days continuously, and detecting the intestinal flora content and the intestinal mucosa antibody content of the mouse after 30 days. (free drinking water for mice during feeding)
TABLE 5 intragastric dosing of NMN in the various example groups
2. And (3) detecting the content of each thallus of the intestinal flora of the mice: for specific operations, refer to step 2 in embodiment group 2, and detailed description is not repeated here. The data results shown in table 6 were obtained.
TABLE 6 bacterial flora and number of cells in the intestinal tract of HFA mice (P < 0.05, P < 0.01, compared to blank)
As can be seen from table 6 above, at a certain NMN dose, the number of bifidobacteria and lactobacilli increased with increasing NMN dose, and the number of enterobacteria and enterococci decreased with increasing NMN dose; after a certain NMN dose, the number of bifidobacteria and lactobacilli decreased with increasing NMN dose, and the number of enterobacteria and enterococci increased with increasing NMN dose. Wherein, when the NMN dose reaches 0.5 g/day, the intestinal bifidobacteria in the mouse are increased from 8.627 + -0.521 to 9.986 + -0.261, the lactobacilli are increased from 8.551 + -0.031 to 9.897 + -0.033, the enterobacteria are decreased from 8.667 + -0.046 to 7.099 + -0.085, and the enterococci are decreased from 7.998 + -0.042 to 7.019 + -0.044. It is demonstrated that NMN can achieve an improvement in intestinal flora.
3. Detecting the content of the mouse intestinal mucosa antibody: for specific operation, refer to step 3 in embodiment group 2, and detailed description is not repeated here. The data results shown in table 7 were obtained.
TABLE 7 HFA mouse intestinal mucosal slg A content (P < 0.05, P < 0.01, compared to blank)
As can be seen from table 7 above, at a certain NMN dose, the cell concentration in the intestinal tract of the mouse increased with the increase of the NMN dose; after a certain NMN dosage is exceeded, the thallus concentration is reduced along with the increase of the NMN dosage. At a certain NMN dose, the content of secreted immunoglobulin (slgA) in the mouse intestinal tract increases with increasing NMN dose; over a certain NMN dose, the secreted immunoglobulin content (slgA) decreased with increasing NMN dose. Wherein the maximum cell concentration is reached at a NMN dose of 0.5 g/day, at which time the OD is reached 450 The value reaches the maximum of0.1279 ± 0.0035 ×; also, the maximum secreted immunoglobulin (slgA) content was reached, which was 6.081 ± 0.0065 × pg/mL.
EXAMPLE group 4
In vivo experiment of combination drug of hydrogen-rich water and NMN on improvement of intestinal flora
1. HFA mice were housed: 40 HFA mice were randomly assigned to 4 groups of 10 mice each, a normal control group. And (3) performing intragastric administration on each mouse according to the dosage of the hydrogen-rich water and NMN combined medicine shown in the table 8, performing intragastric administration 6 times every day for 30 days continuously, and detecting the intestinal flora content and the intestinal mucosa antibody content of the mouse after 30 days. (free drinking water for mouse in feeding process)
TABLE 8 intragastric dosing of the combination of hydrogen-rich water and NMN in each of the example groups
2. Detecting the content of each thallus of the intestinal flora of the mice: for specific operations, refer to step 2 in embodiment group 2 above, and detailed description is not repeated here. The data results shown in table 9 were obtained.
TABLE 9 bacterial flora and number of cells in the gut of HFA mice (P < 0.05, P < 0.01, compared to blank)
As can be seen from table 9 above, at a certain dosage of the combination of NMN and hydrogen-rich water, the number of the cells of bifidobacterium and lactobacillus increases with the increase of the dosage of the combination of NMN and hydrogen-rich water, and the number of the cells of enterobacter and enterococcus decreases with the increase of the dosage of the combination of NMN and hydrogen-rich water; over a certain dosage of the combination of NMN and hydrogen-rich water, the number of the cells of bifidobacterium and lactobacillus decreased with the increase of the dosage of the combination of NMN and hydrogen-rich water, and the number of the cells of enterobacteria and enterococcus increased with the increase of the dosage of the combination of NMN and hydrogen-rich water. Wherein, at a combined drug dose of NMN and hydrogen rich water of 0.5 (g/mL)/day, bifidobacteria in the intestinal tract of mice increased from 8.627 ± 0.521 to 11.645 ± 0.511, lactobacilli increased from 8.551 ± 0.031 to 11.899 ± 0.263, enterobacteria decreased from 8.667 ± 0.046 to 6.019 ± 0.067, and enterococci decreased from 7.998 ± 0.042 to 6.002 ± 0.0552. The result shows that the combination drug of NMN and hydrogen-rich water can improve the intestinal flora and has obvious effect.
3. Detecting the content of the mouse intestinal mucosa antibody: for specific operations, refer to step 3 in embodiment group 2, and detailed description is not repeated here. The data results shown in table 10 were obtained.
TABLE 10 HFA mouse intestinal mucosal slg A content (P < 0.05, P < 0.01, compared to blank)
As can be seen from table 10 above, at a certain dosage of the combination of NMN and hydrogen-rich water, the concentration of the bacteria in the intestinal tract of the mouse increases with the increase of the dosage of the combination of NMN and hydrogen-rich water; after a certain dosage of the combination drug of NMN and hydrogen-rich water is exceeded, the thallus concentration decreases with the increase of the dosage of the combination drug of NMN and hydrogen-rich water. At a certain dosage of the combination of NMN and hydrogen-rich water, the content of secreted immunoglobulin (slgA) in the intestinal tract of the mouse increased with increasing dosage of the combination of NMN and hydrogen-rich water; beyond a certain dosage of the combination of NMN and hydrogen-rich water, the secretory immunoglobulin content (slgA) decreases with increasing dosage of the combination of NMN and hydrogen-rich water. Wherein the maximum cell concentration is reached at a dose of 0.5 (g/mL)/day of the combination of NMN and hydrogen-rich water, at which OD is reached 450 The value reached a maximum of 0.1483 ± 0.0087 ·; the maximum secreted immunoglobulin (slgA) content was also reached, at which time the secreted immunoglobulin content was 6.346 ± 0.0081 ×.
4. Detection of human intestinal flora: 15 healthy volunteers (female, 25 years old, no digestive and metabolic diseases, no antibiotics taken within 90 days) were recruited and scored3 groups of 5 people each, dissolving NMN freeze-dried powder in hydrogen-rich water with the concentration of 0.37mg/ml, 0.75mg/ml and 1.5mg/ml respectively, taking 400ml of NMN hydrogen-rich water solution for each person every day, continuously taking for 30 days, collecting feces before and after administration of volunteers respectively, carrying out 10-time serial dilution to obtain suspension, and then sequentially diluting the suspension according to concentration gradient until the dilution multiple is 10 -5 . Specifically, the sample dilution gradient was bifidobacterium (10) -3 、10 -4 、10 -5 ) Lactobacillus (10) -3 、10 -4 、10 -5 ) Enterobacter (10) -3 、10 -4 、10 -5 ) And enterococcus (10) -3 、10 -4 、10 -5 ). Then, 0.2mL of samples at different dilution concentrations were pipetted onto different selection media, plated and cultured, and 3 sets of parallel experiments were performed at each dilution concentration. The culture conditions and identification methods of different intestinal flora are shown in table 11.
TABLE 11 human intestinal flora culture and identification method
After the completion of the cell culture, the number of viable bacteria was counted by a conventional plate counting method, and the result data shown in Table 12 was obtained.
TABLE 12 bacterial flora and number of cells in human intestinal tract (P < 0.05, P < 0.01, compared to blank)
As can be seen from table 12 above, at a given dose of the combination of NMN and hydrogen-rich water, the number of cells of bifidobacterium and lactobacillus increases with the increase of the dose of the combination of NMN and hydrogen-rich water, and the number of cells of enterobacteria and enterococcus decreases with the increase of the dose of the combination of NMN and hydrogen-rich water; wherein, when the dosage of the NMN and hydrogen-rich water reaches 1.5 (mg/mL)/day, the number of bifidobacteria in human intestinal tract is increased from 7.123 + -0.332 to 9.214 + -0.681, the number of lactobacilli is increased from 6.998 + -0.241 to 9.312 + -0.026, the number of enterobacteria is decreased from 7.603 + -0.323 to 5.997 + -0.527, and the number of coccobacillus is also decreased from 7.383 + -0.243 to 5.893 + -0.0551. The combination drug of NMN and hydrogen-rich water can improve the intestinal flora of human body, and has obvious effect.
The above description is only an alternative embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the present specification and directly/indirectly applied to other related technical fields within the spirit of the present invention are included in the scope of the present invention.
Claims (5)
1. An application of a combined medicine or a health-care product containing hydrogen-rich water and NMN in preparing a medicine for improving intestinal flora is characterized in that the hydrogen content of saturated nanoscale soluble hydrogen molecular groups in the hydrogen-rich water is 0.50-2.0 mg/L; and/or the presence of a gas in the gas,
the pH range of the hydrogen-rich water is 5.85-6.15; and/or the presence of a gas in the gas,
the oxidation-reduction potential of the hydrogen-rich water is-100 to-150 mV;
the combined medicine is characterized in that the mass of NMN dissolved in each 1mL of the hydrogen-rich water is 0.3-2.5 g.
2. The use of claim 1, wherein the single dose amount of the combination medicament is 0.35 to 1.5 mg/mL/day.
3. The use according to claim 1, wherein the combination pharmaceutical or nutraceutical is in liquid form.
4. The use according to claim 3, wherein the combination or nutraceutical is in a form for parenteral administration.
5. The use according to any one of claims 1 to 4, wherein the process for the preparation of a pharmaceutical or nutraceutical combination comprising hydrogen-rich water and NMN comprises: and dissolving the NMN in the hydrogen-rich water, and uniformly mixing to obtain the combined medicine of the hydrogen-rich water and the NMN.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010472676.6A CN111557949B (en) | 2020-05-28 | 2020-05-28 | Medicine or health product containing hydrogen-rich water and/or NMN and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010472676.6A CN111557949B (en) | 2020-05-28 | 2020-05-28 | Medicine or health product containing hydrogen-rich water and/or NMN and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111557949A CN111557949A (en) | 2020-08-21 |
| CN111557949B true CN111557949B (en) | 2022-08-26 |
Family
ID=72069238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010472676.6A Active CN111557949B (en) | 2020-05-28 | 2020-05-28 | Medicine or health product containing hydrogen-rich water and/or NMN and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111557949B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022082487A1 (en) * | 2020-10-21 | 2022-04-28 | 朱上翔 | Anti-aging composite nutrient containing coenzymes and bioactive hydrogen, preparation method therefor and application thereof |
| CN112891241B (en) * | 2021-02-06 | 2022-06-10 | 武汉百思凯瑞生物科技有限公司 | Targeted mitochondrial skin anti-aging nano composition and preparation method and application thereof |
| CN115671178A (en) * | 2021-07-22 | 2023-02-03 | 成都川宇健维生物科技有限公司 | Composition comprising beta-nicotinamide mononucleotide and rosa roxburghii tratt extract and application thereof |
| CN114028422A (en) * | 2021-11-29 | 2022-02-11 | 北京工业大学 | Application of hydrogen molecules in preparation of medicines or foods for regulating intestinal flora |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018195666A1 (en) * | 2017-04-27 | 2018-11-01 | H2 Water Technologies Ltd | Synergistic compositions for increasing mitochondrial function and energy |
| CN110974843A (en) * | 2019-12-23 | 2020-04-10 | 美益添生物医药(武汉)有限公司 | Composition and application thereof in balancing NO producing bacteria ratio in intestinal flora |
| CN113438896A (en) * | 2019-02-12 | 2021-09-24 | 田中惠 | Infant food or beverage, method for improving intestinal environment of infant and method for enhancing immunity of infant |
-
2020
- 2020-05-28 CN CN202010472676.6A patent/CN111557949B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018195666A1 (en) * | 2017-04-27 | 2018-11-01 | H2 Water Technologies Ltd | Synergistic compositions for increasing mitochondrial function and energy |
| CN113438896A (en) * | 2019-02-12 | 2021-09-24 | 田中惠 | Infant food or beverage, method for improving intestinal environment of infant and method for enhancing immunity of infant |
| CN110974843A (en) * | 2019-12-23 | 2020-04-10 | 美益添生物医药(武汉)有限公司 | Composition and application thereof in balancing NO producing bacteria ratio in intestinal flora |
Non-Patent Citations (2)
| Title |
|---|
| "富氢水对模拟失重效应大鼠肠道菌群的影响"，张国文等，《食品科技》，第42卷第05期，第41-45页;张国文等;《食品科技》;20171231;第42卷(第05期);第41-45页 * |
| Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells;Dahyun Hwang等;《Food and Chemical Toxicology》;20181231;第880-888页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111557949A (en) | 2020-08-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111557949B (en) | Medicine or health product containing hydrogen-rich water and/or NMN and preparation method thereof | |
| CN112175864B (en) | Bifidobacterium animalis and breeding method and application thereof | |
| CN110079476B (en) | Lactobacillus fermentum capable of reducing blood uric acid | |
| CN113122467B (en) | Lactobacillus paracasei and composition thereof | |
| WO2020075637A1 (en) | Preventive and/or therapeutic agent for clostridium difficile infection | |
| CN113564078A (en) | Bifidobacterium lactis BLA80 for reducing cholesterol and application thereof | |
| CN100372542C (en) | Use of algin oligocauharide as prebiotics | |
| CN115607575A (en) | Application of Prevotella in prevention and treatment of attention deficit hyperactivity disorder | |
| CN113637603B (en) | Lactobacillus entericus and application thereof | |
| US20060269533A1 (en) | New strains of bifidobacterium having the ability to produce glutamine | |
| CN101623085A (en) | Composition containing probiotics and stachyose | |
| TWI598103B (en) | Composition of lactobacillus fermentum gmnl-296 and method for improving inflection symptoms of clostridium difficile | |
| CN113122471B (en) | Bifidobacterium pseudocatenulatum with high utilization of galactooligosaccharides and application thereof | |
| CN116173075A (en) | Synbiotic composition for improving cognitive function based on clostridium sporogenes and application thereof | |
| CN113397170B (en) | Application of marine prebiotics composition for regulating human intestinal flora | |
| CN115851506A (en) | Lactobacillus rhamnosus JYLR-219 for preventing osteoporosis as well as microbial inoculum and application thereof | |
| CN114836349A (en) | Lactobacillus acidophilus LA16 for antagonizing helicobacter pylori and application thereof | |
| CN113262242A (en) | Application of bifidobacterium lactis JYBR-190 in removing in-vivo heavy metal products | |
| CN110628683A (en) | Probiotic compound live bacterium preparation for preventing and treating chicken diarrhea and preparation method thereof | |
| CN114224941B (en) | Traditional Chinese medicine composition for treating chronic renal failure and application thereof | |
| CN117070425B (en) | Technological method for improving metabolic stability of probiotics in organism and probiotics freeze-dried powder | |
| CN116875500B (en) | Bioactive substance for regulating human body microecological balance prepared from Lactobacillus reuteri YSs207 and application thereof | |
| CN116790402B (en) | Bacteroides simplex strain with anti-inflammatory property, culture method and application | |
| CN116042490B (en) | Composite probiotics for resisting fatigue and improving exercise capacity as well as preparation method and application thereof | |
| CN118021849A (en) | Application of bacillus coagulans in preparation of products for regulating immunity or improving renal function |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |