CN111518695A

CN111518695A - Biological cell culture dish

Info

Publication number: CN111518695A
Application number: CN202010404145.3A
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-13
Filing date: 2020-05-13
Publication date: 2020-08-11

Abstract

The invention belongs to the technical field of microbial culture, and particularly relates to a biological cell culture dish; the culture dish comprises a base, a first fixing ring, a second fixing ring, a first shielding plate, a second shielding plate, a first culture dish, a second culture dish, a rotating shaft and a shell; the culture requirements of different cells under the same environment are met through the matching of the base, the first fixing ring, the second fixing ring, the first shielding plate, the second shielding plate, the first culture dish, the second culture dish, the rotating shaft and the shell; make a sunshade not take place to rotate along with the rotation axis, then take place relative rotation between a sunshade and No. two sunshades this moment to make a through-hole and No. two through-holes intercommunication, the whole air in the culture dish is linked together this moment, thereby has improved the uniformity of cultivateing the environment between a culture dish and No. two culture dishes, has reduced the research error of cultivateing the result, has brought very big just easily for the staff simultaneously.

Description

Biological cell culture dish

Technical Field

The invention belongs to the technical field of microbial culture, and particularly relates to a biological cell culture dish.

Background

The culture dish is a laboratory vessel for culturing microorganisms or cells, consists of a flat disc-shaped bottom and a cover, and is generally made of glass or plastic; the culture dish is basically made of two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells; the plastic may be of polyethylene material, disposable and reusable, suitable for laboratory inoculation, streaking, bacterial isolation and may be used for the cultivation of plant material.

Some solutions for culture dishes have been proposed in the prior art, for example, a chinese patent with application number 2018104959453 discloses a culture dish, which comprises: a transparent cover, a culture part for plant culture and a base; the bottom of the transparent cover covers the culture part, and the culture part is arranged on the base; the edible fungi or vegetables are cultivated through the culture dish, so that pesticide residue and heavy metal residue of the edible fungi or vegetables can be reduced, and the problem of secondary pollution of the edible fungi or vegetables is avoided due to the fact that the edible fungi or vegetables are not required to be transported, and the consumers can obtain edible fungi or vegetables with higher quality; however, the technical scheme fails to meet the culture requirement of different cells under the same environment; in the process of culturing biological cells, different cells may need to be placed in the same culture environment for culturing, but since different cells are cultured in different culture dishes, absolute unification of the environment is difficult to achieve, and sometimes the associativity and antagonism between two cells need to be researched, the culture dishes in the prior art hardly meet the environmental requirements in the research process.

Disclosure of Invention

In order to make up for the deficiency of the prior art, solve the problem that different cells may need to be placed in the same culture environment for culture in the process of culturing biological cells, but because different cells are cultured in different culture dishes, absolute unification of the environment is difficult to realize, and sometimes the associativity and antagonism between two cells need to be researched, the culture dish in the prior art is difficult to meet the environmental requirement in the research process; the invention provides a biological cell culture dish.

The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a biological cell culture dish, which comprises a base, a first fixing ring, a second fixing ring, a first shielding plate, a second shielding plate, a first culture dish, a second culture dish, a rotating shaft and a shell, wherein the first fixing ring is arranged on the base; the first fixing ring is arranged on the base, and an annular groove is formed in the side wall of the top end of the first fixing ring; the first culture dish is arranged on the base in the first fixing ring; the first shielding plate is arranged on the side wall of the top end of the first culture dish, and a first through hole is formed in the first shielding plate; the bottom end of the second fixing ring is provided with a sliding block, and the second fixing ring is connected in the annular groove in a sliding manner through the sliding block; the second shielding plate is rotatably connected to the side wall of the top end of the first shielding plate, a support column is arranged on the side wall of the top end of the second shielding plate, and a second through hole is formed in the second shielding plate; the second culture dish is arranged on the support; the bottom end of the rotating shaft is rotatably connected to the base, the top end of the rotating shaft penetrates through the first shielding plate and the second shielding plate and then extends to the side wall of the top end of the second culture dish, the side wall of the rotating shaft is rotatably connected with the first shielding plate, and the side wall of the rotating shaft is fixedly connected with the second shielding plate; the shell is sleeved on the base, the top end of the shell is rotatably connected with a knob, and the bottom end of the knob penetrates into the shell to be matched with the top end of the rotating shaft; the culture requirements of different cells under the same environment are met through the matching of the base, the first fixing ring, the second fixing ring, the first shielding plate, the second shielding plate, the first culture dish, the second culture dish, the rotating shaft and the shell; when the culture medium is used, different biological cells are respectively placed in a first culture dish and a second culture dish through culture solution for culture, in the culture process, if the two culture dishes are required to be in the same culture environment, the rotating shaft is driven to rotate through the knob on the rotating shell, and in the rotating process of the rotating shaft, the second shielding plate is fixedly connected to the side wall of the rotating shaft, so that the second shielding plate rotates along with the rotating shaft; because the first shielding plate is connected to the side wall of the rotating shaft in a rotating mode, the first shielding plate does not rotate along with the rotating shaft, relative rotation occurs between the first shielding plate and the second shielding plate at the moment, the first through hole is communicated with the second through hole, and the whole air in the culture dish is communicated at the moment, so that the consistency of the culture environment between the first culture dish and the second culture dish is improved, the research error of a culture result is reduced, and meanwhile, the great convenience and easiness are brought to workers.

Preferably, the side wall of the knob is provided with an air bag; a fixed rod is arranged at the top end of the rotating shaft; a cavity is formed in the fixing rod; the cavity is communicated with the air bag through an air pipe, a piston rod and a blocking piece are connected in the cavity in a sliding mode, the piston rod is close to the air bag, the blocking piece is far away from the air bag, and the piston rod is in contact with the air bag; the stability of the culture solution in the second culture dish is improved through the matching of the air bag, the fixing rod, the piston rod and the baffle plate; when the culture dish is used, in the culture process, if the two culture dishes are required to be ensured to be in the same culture environment, the rotating shaft is driven to rotate through the knob on the rotating shell, when the knob is rotated, a worker extrudes the air bag, after the air bag is pressed, the air in the air bag is transmitted into the cavity through the air pipe, so that after the air pressure in the cavity is increased, the piston rod slides in the cavity in the direction away from the knob, the piston rod generates thrust on the baffle plate in the sliding process, the baffle plate slides out of the cavity after being subjected to the thrust and covers the surface of the second culture dish, the baffle plate can generate resistance on the culture liquid in the second culture dish, the vibration frequency of the culture liquid is reduced, when the second culture dish rotates along with the second baffle plate, the vibration synchronism between the culture liquid and the second culture dish is changed, the stability of the surface of the culture liquid is effectively ensured, and the second culture dish is prevented from rotating, the condition that the culture solution spills from No. two culture dishes avoids the relatively poor pollution between the culture solution, improves the holistic safety in utilization of culture dish.

Preferably, the blocking piece consists of a first elastic piece, a second elastic piece and blocking cloth; the first elastic sheet and the second elastic sheet have bending angles, and the bending direction of the first elastic sheet and the bending direction of the second elastic sheet are far away from each other; two sides of the blocking cloth are respectively arranged on the side walls of the first elastic sheet and the second elastic sheet which are close to each other; the covering effect on the culture solution is enhanced through the matching of the first elastic sheet, the second elastic sheet and the blocking cloth; during the use, in the culture process, when needing to guarantee that two culture dishes are in the same cultivation environment, through the turn-knob on the rotatory casing, drive the rotation axis and rotate, when rotatory turn-knob, the staff produces the extrusion to the gasbag, gasbag pressurized back, its inside gas passes through in trachea transmission to the cavity, make behind the increase of cavity internal gas pressure, the piston rod slides to the direction of keeping away from the turn-knob in the cavity, the piston rod produces thrust to the separation blade at the slip in-process, the separation blade receives behind the thrust and slides out in the cavity, at the in-process of separation blade roll-off, elastic deformation that first shell fragment and No. two shell fragments took place to keep away from each other under the spring action of self, pull up the fender cloth when first shell fragment and No. two shell fragments warp, thereby realized the full coverage to No. two culture dish surfaces, the stability of culture solution in No. two culture dishes has further been guaranteed.

Preferably, the surface of the cloth baffle is provided with a polytetrafluoroethylene coating, the surface of the cloth baffle is provided with a connecting rod, and the side wall of the connecting rod is symmetrically provided with a plurality of supporting rods; the end part of the connecting rod is connected with the side wall of the piston rod; the stretching degree of the blocking cloth is enhanced through the matching of the piston rod, the blocking cloth, the connecting rod and the supporting rod, and the blocking cloth is prevented from being blocked when the blocking piece is recovered; when in use, after the second shield plate is rotated, the worker loosens the knob, the air bag resets under the action of the self elasticity, the piston rod also resets, no pressure is generated on the first elastic sheet and the second elastic sheet, the first elastic sheet and the second elastic sheet are reset under the self elastic action and are retracted into the cavity again, but in the resetting process of the first elastic sheet and the second elastic sheet, because the blocking cloth is soft in texture and low in plasticity, the blocking cloth can be clamped in the cavity, so that the normal contraction effect of the first elastic sheet and the second elastic sheet is influenced, therefore, in the resetting process of the piston rod, the connecting rod generates tension on the supporting rod, and then the supporting rod generates contraction force on the whole blocking cloth, thereby effectively guaranteed when the separation blade contracts that the fender cloth can be saved between shell fragment and No. two shell fragments, further strengthened the whole result of use of culture dish.

Preferably, a first magnet is mounted at the end part of the baffle plate; a second magnet is arranged on the side wall of the second culture dish, and mutual attraction exists between the first magnet and the second magnet; the stability of the blocking piece is enhanced through the matching of the first magnet and the second magnet; when the device is used, in the culture process, if the two culture dishes are required to be ensured to be in the same culture environment, the rotating shaft is driven to rotate through the knob on the rotating shell, when the knob is rotated, a worker extrudes the air bag, after the air bag is pressed, the air in the air bag is transmitted into the cavity through the air pipe, so that the air pressure in the cavity is increased, the piston rod slides in the cavity in the direction away from the knob, the piston rod generates thrust on the blocking piece in the sliding process, the blocking piece slides out of the cavity after being subjected to the thrust, in the process of sliding out of the blocking piece, the first elastic piece and the second elastic piece generate elastic deformation which is away from each other under the action of the self elasticity, the blocking cloth is pulled up when the first elastic piece and the second elastic piece deform, thereby the full coverage on the surface of the second culture dish is realized, and under the action of the mutual attraction force between the first magnet and the second magnet, the first elastic piece and the second elastic piece are respectively in close contact with the, after the culture dish service time overlength, the elasticity of a shell fragment and No. two shell fragments takes place to lose efficacy after, influences the cover effect to the culture solution to the life of culture dish has effectively been prolonged.

Preferably, the end of the fixing rod is tapered to facilitate the engagement between the knob and the fixing rod; during the use, place different biological cells in culture dish and No. two culture dishes through the culture solution respectively and cultivate, then cover the casing outside culture dish and No. two culture dishes, and make the dead lever cooperate with the turn-knob, so that subsequent gas transmission and revolving force transmission, but because at the in-process of covering the casing, the staff can't look at the casing inner structure directly, thereby increased the degree of difficulty for the cooperation between turn-knob and the dead lever, consequently establish the fixed rod tip to the toper, thereby make the cooperation between realization turn-knob and the dead lever that the staff can be easier, effectively increased the holistic practicality of culture dish, brought very big convenience for the staff simultaneously.

The invention has the following beneficial effects:

1. according to the biological cell culture dish, the culture requirements of different cells under the same environment are met through the matching of the base, the first fixing ring, the second fixing ring, the first shielding plate, the second shielding plate, the first culture dish, the second culture dish, the rotating shaft and the shell, and after the first through hole is communicated with the second through hole, the whole air in the culture dish is communicated, so that the consistency of the culture environment between the first culture dish and the second culture dish is improved, the research error of a culture result is reduced, and meanwhile, great convenience and easiness are brought to workers.

2. According to the biological cell culture dish, the stability of the culture solution in the second culture dish is improved through the matching of the air bag, the fixing rod, the piston rod and the baffle plate, and the baffle plate can generate resistance to the culture solution in the second culture dish, so that the vibration frequency of the culture solution is reduced, the vibration synchronism between the culture solution and the second culture dish is changed when the second culture dish rotates along with the second baffle plate, the stability of the surface of the culture solution is effectively ensured, the situation that the culture solution is splashed out of the second culture dish in the rotating process of the second culture dish is avoided, the poor pollution between the culture solution is avoided, and the use safety of the whole culture dish is improved.

Drawings

The invention will be further explained with reference to the drawings.

FIG. 1 is a perspective view of the present invention;

FIG. 2 is a cross-sectional view of the present invention;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

FIG. 4 is an enlarged view of a portion of FIG. 2 at B;

FIG. 5 is an enlarged view of a portion of FIG. 2 at C;

FIG. 6 is a schematic view of the structure of the baffle plate of the present invention;

in the figure: base 1, No. one fixed ring 2, annular groove 21, No. two fixed ring 3, slider 31, a sunshade 4, a through-hole 41, No. two sunshade 5, pillar 51, No. two through-holes 52, a culture dish 6, No. two culture dishes 7, No. two magnet 71, rotation axis 8, dead lever 81, cavity 82, piston rod 83, separation blade 84, an elastic sheet 841, No. two elastic sheets 842, fender cloth 843, connecting rod 844, branch 845, a magnet 846, casing 9, turn knob 91, gasbag 92.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1 to 6, a biological cell culture dish according to the present invention includes a base 1, a first fixing ring 2, a second fixing ring 3, a first shielding plate 4, a second shielding plate 5, a first culture dish 6, a second culture dish 7, a rotating shaft 8, and a housing 9; the first fixing ring 2 is arranged on the base 1, and the side wall of the top end of the first fixing ring 2 is provided with an annular groove 21; the first culture dish 6 is arranged on the base 1 in the first fixing ring 2; the first shielding plate 4 is arranged on the side wall of the top end of the first culture dish 6, and a first through hole 41 is formed in the first shielding plate 4; the bottom end of the second fixing ring 3 is provided with a sliding block 31, and the second fixing ring 3 is connected in the annular groove 21 in a sliding manner through the sliding block 31; the second shielding plate 5 is rotatably connected to the side wall of the top end of the first shielding plate 4, a support 51 is arranged on the side wall of the top end of the second shielding plate 5, and a second through hole 52 is formed in the second shielding plate 5; the second culture dish 7 is arranged on the support column 51; the bottom end of the rotating shaft 8 is rotatably connected to the base 1, the top end of the rotating shaft 8 penetrates through the first shielding plate 4 and the second shielding plate 5 and then extends to the side wall of the top end of the second culture dish 7, the side wall of the rotating shaft 8 is rotatably connected with the first shielding plate 4, and the side wall of the rotating shaft 8 is fixedly connected with the second shielding plate 5; the shell 9 is sleeved on the base 1, the top end of the shell 9 is rotatably connected with a knob 91, and the bottom end of the knob 91 penetrates into the shell 9 to be matched with the top end of the rotating shaft 8; the culture requirements of different cells under the same environment are realized through the cooperation of the base 1, the first fixing ring 2, the second fixing ring 3, the first shielding plate 4, the second shielding plate 5, the first culture dish 6, the second culture dish 7, the rotating shaft 8 and the shell 9; when the culture medium is used, different biological cells are respectively placed in the first culture dish 6 and the second culture dish 7 through culture solution for culture, in the culture process, if the two culture dishes are required to be in the same culture environment, the rotating shaft 8 is driven to rotate through the knob 91 on the rotating shell 9, and in the rotating process of the rotating shaft 8, the second shielding plate 5 is fixedly connected to the side wall of the rotating shaft 8, so that the second shielding plate 5 rotates along with the rotating shaft 8; because No. one sunshade 4 rotates and connects on the rotation axis 8 lateral wall again for No. one sunshade 4 does not take place to rotate along with rotation axis 8, then take place relative rotation between No. one sunshade 4 and No. two sunshades 5 this moment, thereby make No. one through-hole 41 and No. two through-holes 52 communicate, the whole air in the culture dish is linked together this moment, thereby improved the uniformity of culture environment between No. one culture dish 6 and No. two culture dishes 7, reduced the research error of culture result, brought very big convenient and easy simultaneously for the staff.

In one embodiment of the present invention, an air bag 92 is mounted on the side wall of the knob 91; a fixed rod 81 is arranged at the top end of the rotating shaft 8; a cavity 82 is formed in the fixing rod 81; the cavity 82 is communicated with the air bag 92 through an air pipe, a piston rod 83 and a baffle 84 are connected in the cavity 82 in a sliding mode, the piston rod 83 is close to the air bag 92, the baffle 84 is far away from the air bag 92, and the piston rod 83 is in contact with the air bag 92; the stability of the culture solution in the second culture dish 7 is improved through the matching of the air bag 92, the fixing rod 81, the piston rod 83 and the baffle 84; when the culture dish is used, in the culture process, if the two culture dishes are required to be ensured to be in the same culture environment, the rotating shaft 8 is driven to rotate by rotating the knob 91 on the shell 9, when the knob 91 is rotated, a worker extrudes the air bag 92, after the air bag 92 is compressed, the air in the air bag 92 is transmitted into the cavity 82 through the air pipe, so that after the air pressure in the cavity 82 is increased, the piston rod 83 slides in the direction away from the knob 91 in the cavity 82, the piston rod 83 generates thrust on the baffle plate 84 in the sliding process, the baffle plate 84 slides out of the cavity 82 after being pushed and covers the surface of the second culture dish 7, the baffle plate 84 can generate resistance on the culture liquid in the second culture dish 7, the vibration frequency of the culture liquid is reduced, and when the second culture dish 7 rotates along with the second baffle plate 5, the vibration synchronism between the culture liquid and the second culture dish 7 is changed, effectively guaranteed the stability on culture solution surface, avoided No. two culture dishes 7 at rotatory in-process, the relatively poor pollution between the culture solution is avoided to the condition that the culture solution spills from No. two culture dishes 7, improves the holistic safety in utilization of culture dish.

As an embodiment of the present invention, the blocking sheet 84 is composed of a first elastic sheet 841, a second elastic sheet 842 and a blocking cloth 843; the first elastic sheet 841 and the second elastic sheet 842 have bending angles, and the bending direction of the first elastic sheet 841 and the bending direction of the second elastic sheet 842 are far away from each other; two sides of the blocking cloth 843 are respectively arranged on the side walls of the first elastic sheet 841 and the second elastic sheet 842 which are close to each other; the covering effect on the culture solution is enhanced through the cooperation of the first elastic sheet 841, the second elastic sheet 842 and the blocking cloth 843; when in use, in the process of culture, if the two culture dishes are required to be kept in the same culture environment, the rotating shaft 8 is driven to rotate by rotating the knob 91 on the shell 9, when the knob 91 is rotated, the worker presses the air bag 92, and after the air bag 92 is pressed, the air inside the cavity 82 is transmitted into the cavity 82 through the air pipe, so that after the air pressure in the cavity 82 is increased, the piston rod 83 slides in the cavity 82 in the direction away from the knob 91, the piston rod 83 generates thrust to the blocking piece 84 in the sliding process, the blocking piece 84 slides out of the cavity 82 after being pushed, in the process of sliding out the blocking piece 84, the first elastic sheet 841 and the second elastic sheet 842 elastically deform away from each other under the action of the elastic force of the first elastic sheet 841 and the second elastic sheet 842, the blocking cloth 843 is pulled up when the first elastic sheet 841 and the second elastic sheet 842 deform, so that the surface of the second culture dish 7 is completely covered, and the stability of the culture solution in the second culture dish 7 is further ensured.

As an embodiment of the invention, a polytetrafluoroethylene coating is arranged on the surface of the cloth 843, a connecting rod 844 is arranged on the surface of the cloth 843, and a plurality of struts 845 are symmetrically arranged on the side wall of the connecting rod 844; the end of the connecting rod 844 is connected with the side wall of the piston rod 83; the stretching degree of the blocking cloth 843 is enhanced through the matching of the piston rod 83, the blocking cloth 843, the connecting rod 844 and the supporting rod 845, and meanwhile, the blocking situation of the blocking cloth 843 is avoided when the blocking piece 84 is recovered; when the folding structure is used, after the rotation of the second shielding plate 5 is completed, a worker loosens the knob 91, the air bag 92 resets under the action of the elastic force of the air bag 92, the piston rod 83 also resets, no pressure is generated on the first elastic sheet 841 and the second elastic sheet 842, the first elastic sheet 841 and the second elastic sheet 842 also reset under the action of the elastic force of the air bag, and the air bag retracts into the cavity 82 again, but in the resetting process of the first elastic sheet 841 and the second elastic sheet 842, because the blocking cloth 843 is soft in texture and low in plasticity, the blocking cloth 843 is clamped in the cavity 82, so that the normal retracting effect of the first elastic sheet 841 and the second elastic sheet 842 is influenced, therefore, in the resetting process of the piston rod 83, the connecting rod 844 generates pulling force on the supporting rod 845, and then the supporting rod 845 generates a retracting force on the whole blocking cloth 843, so that the blocking cloth 843 can be stored between the first elastic sheet 841 and the second elastic sheet 842 when the blocking plate 84 contracts, further enhancing the overall use effect of the culture dish.

In one embodiment of the present invention, a first magnet 846 is installed at the end of the blocking piece 84; the second magnet 71 is arranged on the side wall of the second culture dish 7, and the first magnet 846 and the second magnet 71 have mutual attraction; the stability of the blocking piece 84 is enhanced through the matching of the first magnet 846 and the second magnet 71; when the culture dish is used, in the culture process, if the two culture dishes are required to be in the same culture environment, the rotating shaft 8 is driven to rotate by rotating the knob 91 on the shell 9, when the knob 91 is rotated, a worker extrudes the air bag 92, after the air bag 92 is pressed, the air in the air bag 92 is transmitted into the cavity 82 through the air pipe, so that after the air pressure in the cavity 82 is increased, the piston rod 83 slides in the direction away from the knob 91 in the cavity 82, the piston rod 83 generates thrust on the baffle plate 84 in the sliding process, the baffle plate 84 slides out of the cavity 82 after being pushed, in the sliding process of the baffle plate 84, the first elastic sheet 841 and the second elastic sheet 842 generate mutual-away elastic deformation under the action of the elastic force of the first elastic sheet 841 and the second elastic sheet 842, the baffle cloth 843 is pulled up when the first elastic sheet 841 and the second elastic sheet 842 deform, thereby realizing the full coverage of the surface of the second culture dish 7, and under the action of the mutual attraction force between the first magnet 846 and the second, no. one shell fragment 841 and No. two shell fragments 842 respectively with No. two 7 lateral walls in close contact with of culture dish, avoid the culture dish live time overlength after, the elasticity of a shell fragment 841 and No. two shell fragments 842 takes place to become invalid, influences the covering effect to the culture solution to the life of culture dish has effectively been prolonged.

As an embodiment of the present invention, the end of the fixing lever 81 is tapered, thereby facilitating the engagement between the knob 91 and the fixing lever 81; during the use, place different biological cells in culture dish 6 and No. two culture dishes 7 through the culture solution respectively and cultivate, then cover casing 9 outside culture dish 6 and No. two culture dishes 7, and make dead lever 81 cooperate with knob 91, so that subsequent gas transmission and revolving force transmission, but because at the in-process of covering casing 9, the staff can't look directly at casing 9 inner structure, thereby increased the degree of difficulty for the cooperation between knob 91 and dead lever 81, consequently establish the dead lever 81 tip to the toper, thereby make the cooperation between realization knob 91 and the dead lever 81 that the staff can be easier, effectively increased the holistic practicality of culture dish, brought very big convenience for the staff simultaneously.

When the culture medium is used, different biological cells are respectively placed in the first culture dish 6 and the second culture dish 7 through culture solution for culture, in the culture process, if the two culture dishes are required to be in the same culture environment, the rotating shaft 8 is driven to rotate through the knob 91 on the rotating shell 9, and in the rotating process of the rotating shaft 8, the second shielding plate 5 is fixedly connected to the side wall of the rotating shaft 8, so that the second shielding plate 5 rotates along with the rotating shaft 8; the first shielding plate 4 is rotatably connected to the side wall of the rotating shaft 8, so that the first shielding plate 4 does not rotate along with the rotating shaft 8, the first shielding plate 4 and the second shielding plate 5 rotate relatively at the moment, the first through hole 41 is communicated with the second through hole 52, and the whole air in the culture dish is communicated at the moment, so that the consistency of the culture environment between the first culture dish 6 and the second culture dish 7 is improved, the research error of a culture result is reduced, and meanwhile, the great convenience and easiness are brought to workers; in the culture process, if it is required to ensure that the two culture dishes are in the same culture environment, the rotating shaft 8 is driven to rotate by rotating the knob 91 on the housing 9, when the knob 91 is rotated, the worker extrudes the air bag 92, after the air bag 92 is compressed, the air in the air bag is transmitted into the cavity 82 through the air pipe, so that after the air pressure in the cavity 82 is increased, the piston rod 83 slides in the cavity 82 in the direction away from the knob 91, the piston rod 83 generates thrust on the baffle 84 in the sliding process, the baffle 84 slides out of the cavity 82 after being pushed and covers the surface of the second culture dish 7, the baffle 84 generates resistance on the culture solution in the second culture dish 7, thereby reducing the vibration frequency of the culture solution, so that when the second culture dish 7 rotates along with the second shield plate 5, the vibration synchronism between the culture solution and the second culture dish 7 is changed, and the stability of the surface of the culture solution is effectively ensured, the situation that the culture solution is splashed out of the second culture dish 7 in the rotating process of the second culture dish 7 is avoided, poor pollution among the culture solutions is avoided, and the use safety of the whole culture dish is improved; in the culture process, if the two culture dishes are required to be kept in the same culture environment, the rotary shaft 8 is driven to rotate by rotating the knob 91 on the shell 9, when the knob 91 is rotated, the worker presses the air bag 92, and after the air bag 92 is pressed, the air inside the cavity 82 is transmitted into the cavity 82 through the air pipe, so that after the air pressure in the cavity 82 is increased, the piston rod 83 slides in the cavity 82 in the direction away from the knob 91, the piston rod 83 generates thrust to the blocking piece 84 in the sliding process, the blocking piece 84 slides out of the cavity 82 after being pushed, in the process of sliding out the blocking piece 84, the first elastic sheet 841 and the second elastic sheet 842 elastically deform away from each other under the action of the elastic force of the first elastic sheet 841 and the second elastic sheet 842, the blocking cloth 843 is pulled up when the first elastic sheet 841 and the second elastic sheet 842 deform, so that the surface of the second culture dish 7 is completely covered, and the stability of the culture solution in the second culture dish 7 is further ensured; after the rotation of the second shield plate 5 is completed, the worker loosens the knob 91, the air bag 92 resets under the action of the elastic force of the air bag, the piston rod 83 resets, and no pressure is generated on the first elastic sheet 841 and the second elastic sheet 842, so that the first elastic sheet 841 and the second elastic sheet 842 reset under the action of the elastic force of the air bag, and the piston rod 83 retracts into the cavity 82 again, but in the resetting process of the first elastic sheet 841 and the second elastic sheet 842, because the blocking cloth 843 is soft in texture and low in plasticity, the blocking cloth 843 is clamped in the cavity 82, so that the normal contraction effect of the first elastic sheet 841 and the second elastic sheet 842 is influenced, therefore, in the resetting process of the piston rod 83, the connecting rod 844 generates a pulling force on the supporting rod 845, and then generates a convergence force on the whole blocking cloth 843 through the supporting rod 845, so that the blocking cloth 843 can be stored between the first elastic sheet 841 and the second elastic sheet 842 when the blocking cloth 84 contracts, the overall use effect of the culture dish is further enhanced; in the culture process, if it is required to ensure that the two culture dishes are in the same culture environment, the rotating shaft 8 is driven to rotate by rotating the knob 91 on the housing 9, when the knob 91 is rotated, the worker extrudes the air bag 92, after the air bag 92 is compressed, the air in the air bag is transmitted into the cavity 82 through the air pipe, so that after the air pressure in the cavity 82 is increased, the piston rod 83 slides in the cavity 82 in the direction away from the knob 91, the piston rod 83 generates thrust on the blocking piece 84 in the sliding process, the blocking piece 84 slides out of the cavity 82 after being thrust, in the process that the blocking piece 84 slides out, the first elastic piece 841 and the second elastic piece 842 generate elastic deformation which is away from each other under the action of the own elastic force, the blocking cloth 843 is pulled up when the first elastic piece 841 and the second elastic piece 842 deform, thereby realizing the full coverage of the surface of the second culture dish 7, and under the action of the mutual attraction force between the first magnet 846 and the second magnet 71, the first elastic sheet 841 and the second elastic sheet 842 are respectively in close contact with the side wall of the second culture dish 7, so that the phenomenon that the covering effect on the culture solution is influenced after the elasticity of the first elastic sheet 841 and the second elastic sheet 842 is invalid after the culture dish is used for too long is avoided, and the service life of the culture dish is effectively prolonged; different biological cells are respectively placed in a culture dish 6 and a culture dish 7 through culture solution for culture, then a shell 9 is covered outside the culture dish 6 and the culture dish 7, and the fixing rod 81 is matched with the knob 91, so that the subsequent gas transmission and the rotating force transmission are facilitated, but because the process of covering the shell 9 is adopted, the worker can not look at the internal structure of the shell 9, the difficulty is increased for the matching between the knob 91 and the fixing rod 81, the end part of the fixing rod 81 is set to be conical, so that the worker can more easily realize the matching between the knob 91 and the fixing rod 81, the overall practicability of the culture dish is effectively increased, and meanwhile, great convenience is brought to the worker.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A biological cell culture dish, characterized in that: comprises a base (1), a first fixing ring (2), a second fixing ring (3), a first shielding plate (4), a second shielding plate (5), a first culture dish (6), a second culture dish (7), a rotating shaft (8) and a shell (9); the first fixing ring (2) is arranged on the base (1), and an annular groove (21) is formed in the side wall of the top end of the first fixing ring (2); the first culture dish (6) is arranged on the base (1) in the first fixing ring (2); the first shielding plate (4) is arranged on the side wall of the top end of the first culture dish (6), and a first through hole (41) is formed in the first shielding plate (4); the bottom end of the second fixing ring (3) is provided with a sliding block (31), and the second fixing ring (3) is connected in the annular groove (21) in a sliding mode through the sliding block (31); the second shielding plate (5) is rotatably connected to the side wall of the top end of the first shielding plate (4), a support column (51) is arranged on the side wall of the top end of the second shielding plate (5), and a second through hole (52) is formed in the second shielding plate (5); the second culture dish (7) is arranged on the support column (51); the bottom end of the rotating shaft (8) is rotatably connected to the base (1), the top end of the rotating shaft (8) penetrates through the first shielding plate (4) and the second shielding plate (5) and then extends to the side wall of the top end of the second culture dish (7), the side wall of the rotating shaft (8) is rotatably connected with the first shielding plate (4), and the side wall of the rotating shaft (8) is fixedly connected with the second shielding plate (5); the shell (9) is sleeved on the base (1), the top end of the shell (9) is rotatably connected with a knob (91), and the bottom end of the knob (91) penetrates into the shell (9) to be matched with the top end of the rotating shaft (8); the culture requirements of different cells under the same environment are realized through the matching of the base (1), the first fixing ring (2), the second fixing ring (3), the first shielding plate (4), the second shielding plate (5), the first culture dish (6), the second culture dish (7), the rotating shaft (8) and the shell (9).

2. A biological cell culture dish according to claim 1, wherein: an air bag (92) is mounted on the side wall of the knob (91); a fixed rod (81) is installed at the top end of the rotating shaft (8); a cavity (82) is formed in the fixing rod (81); the cavity (82) is communicated with the air bag (92) through an air pipe, a piston rod (83) and a blocking piece (84) are connected in the cavity (82) in a sliding mode, the piston rod (83) is close to the air bag (92), the blocking piece (84) is far away from the air bag (92), and the piston rod (83) is in contact with the air bag (92); the stability of the culture solution in the second culture dish (7) is improved through the matching of the air bag (92), the fixing rod (81), the piston rod (83) and the baffle plate (84).

3. A biological cell culture dish according to claim 2, wherein: the baffle plate (84) consists of a first elastic sheet (841), a second elastic sheet (842) and a blocking cloth (843); the first elastic sheet (841) and the second elastic sheet (842) have bending angles, and the bending direction of the first elastic sheet (841) and the bending direction of the second elastic sheet (842) are far away from each other; two sides of the blocking cloth (843) are respectively arranged on the side walls of the first elastic sheet (841) and the second elastic sheet (842) which are close to each other; the covering effect on the culture solution is enhanced through the cooperation of the first elastic sheet (841), the second elastic sheet (842) and the blocking cloth (843).

4. A biological cell culture dish according to claim 3, wherein: the surface of the blocking cloth (843) is provided with a polytetrafluoroethylene coating, the surface of the blocking cloth (843) is provided with a connecting rod (844), and the side wall of the connecting rod (844) is symmetrically provided with a plurality of supporting rods (845); the end of the connecting rod (844) is connected with the side wall of the piston rod (83); the spreading degree of the blocking cloth (843) is enhanced through the matching of the piston rod (83), the blocking cloth (843), the connecting rod (844) and the supporting rod (845), and meanwhile, the blocking situation of the blocking cloth (843) when the blocking piece (84) is recovered is avoided.

5. A biological cell culture dish according to claim 3, wherein: a first magnet (846) is installed at the end part of the baffle plate (84); a second magnet (71) is arranged on the side wall of the second culture dish (7), and mutual attraction exists between the first magnet (846) and the second magnet (71); the stability of the blocking piece (84) is enhanced through the matching of the first magnet (846) and the second magnet (71).

6. A biological cell culture dish according to claim 2, wherein: the end of the fixing rod (81) is tapered so as to facilitate the cooperation between the knob (91) and the fixing rod (81).