CN111514367B - Wound adhesive hydrogel material, preparation method and application thereof - Google Patents
Wound adhesive hydrogel material, preparation method and application thereof Download PDFInfo
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Abstract
A wound adhesive hydrogel material, a preparation method and application thereof. The main components of the wound adhesive hydrogel material comprise polyacrylic acid, chitosan, tannic acid and a chelating agent, wherein the polyacrylic acid and the chitosan form an interpenetrating network through electrostatic interaction; tannic acid to enhance the viscosity and acidity of the wound-adhesive hydrogel; and the chelating agent is used for crosslinking the tannic acid with the polyacrylic acid and the chitosan in the interpenetrating network. The wound adhesive hydrogel material has high adhesive strength in air and water, can realize repeated adhesion, is stable in adhesion, is not influenced by acid and alkali, can specifically adhere to biological soft tissues in water, and has antibacterial and obvious healing promotion capabilities on wounds. The stable bonding strength, the high bonding efficiency and the repeated bonding greatly save the operation time, the specific adhesion to the biological soft tissue underwater is convenient for the operation of doctors, and the pain of patients is reduced. Besides, the wearable device has the potential to be used as a base of the wearable device.
Description
Technical Field
The invention relates to a wound adhesive hydrogel material with biological soft tissue super-adhesion and antibacterial functions, a preparation method and application thereof, and belongs to the technical field of biomedical materials.
Background
In recent years, many emerging biomedical materials have been used for wound or wound healing, including porous sponges, electrospun, biofilms, and the like. How to rapidly stop bleeding and promote tissue self-repair becomes the focus of attention of material design. However, the materials need to be bound in actual operation, the wound treatment process is professional, the demand for convenient and non-traumatic adhesive tapes such as band-aids is increasing day by day, and the viscous hydrogel materials gradually come into the visual field of people.
The hydrogel material is a water-dispersed polymer chain, has excellent air permeability, but the wound is in an open state and is easy to infect, so that the hydrogel material is necessary to be endowed with antibacterial capacity. In addition, the sweat glands on the skin are developed, the surface is usually covered with sweat and grease, the adhesion of the moist tissue surface to the hydrogel is very unfavorable, and most hydrogel dressings do not have long-term repeatable adhesiveness at present. Therefore, it is desired to develop an antimicrobial hydrogel that can maintain a cyclic viscosity on a wet or grease-covered surface.
After the common viscous hydrogel material interacts with water, the surface and the water form hydrogen bonds, so that the interaction of the material and other substances is reduced, and the viscosity is reduced or even lost. The key to solving the problem of wet surface adhesion is to make the material surface recover viscosity, i.e. maintain a certain hydrogen bond density on the material surface. Polyacrylic acid has many carboxyl groups and can interact with many substances to form hydrogen bonds, thereby having viscosity, and particularly interacting with amino groups in tissues. The partially dissociated carboxylate radicals can interact with positive electricity on the amino group of the chitosan, strong electrostatic interaction is provided, the relative stability of the structure is maintained, the interaction between the surface carboxyl group of the chitosan and water is inhibited, after the chitosan is contacted with water molecules, the protonated chitosan releases hydrogen bonds between the water and the carboxyl group through electrostatic interaction through slight extrusion, so that the water molecules are discharged, the density of the hydrogen bonds on the surface is maintained, and the chitosan has underwater adhesion performance.
Tannic acid has an o-phenolic dihydroxy structure similar to dopamine, which is the main sticky substance in mussels, and a biphenyl structure provides stronger conjugation, so that the adhesion performance of the tannic acid is better than that of the dopamine. The tannic acid also has certain oxidation resistance and antibacterial property, can remove free radicals at wounds, can inhibit wound infection and limit inflammatory reaction of the wounds, and thus can promote wound repair more effectively. The strong reducing property of tannic acid causes it to be easily resistant to polymerization, thus limiting the content thereof in polyacrylic acid hydrogel. The protonated chitosan can bind to phenolic hydroxyl groups on tannic acid, thereby preventing tannic acid from oxidizing and reducing its polymerization inhibiting effect.
Disclosure of Invention
The invention aims to provide a wound adhesive hydrogel material with biological soft tissue super-adhesion and antibacterial functions, a preparation method and application thereof.
To achieve the above object, the present invention provides a wound-adhesive hydrogel material comprising polyacrylic acid, chitosan, tannic acid and a chelating agent as main components, wherein: polyacrylic acid and chitosan form an interpenetrating network through electrostatic interaction; tannic acid to enhance the viscosity and acidity of the wound-adhesive hydrogel; and the chelating agent is used for crosslinking the tannic acid with the polyacrylic acid and the chitosan in the interpenetrating network.
In the above scenario, the polyacrylic acid serves as the primary network in the wound-adhesive hydrogel material, and the mass fraction in the wound-adhesive hydrogel material is 19% for providing substantial mechanical strength and substantial tack.
In the above scheme, the chitosan is used as a secondary network in the wound-adhesive hydrogel material, and the mass fraction of the chitosan in the wound-adhesive hydrogel material is 2-3%; the chitosan is protonated under the acidic condition of polyacrylic acid, inhibits the oxidation of tannic acid, reduces the inhibition effect of tannic acid, and acts with negatively charged groups on the surface of a tissue to improve the viscosity.
In the scheme, the tannic acid is used for assisting crosslinking, and the mass fraction of the tannic acid in the wound binding hydrogel material is 1-3%.
In the above scheme, the chelating agent is Al-containing3+The inorganic salt of (1), the Al-containing3+The inorganic salt of (A) is Al (NO)3)3·9H2O or AlCl3。
In the above aspect, the wound-adhesive hydrogel material further includes: methylene acrylamide BIS, used to crosslink acrylic acid.
In order to achieve the above another object, the present invention further provides a method for preparing the wound-adhesive hydrogel material, which adopts a one-pot polymerization method, specifically comprising:
forming an interpenetrating network by utilizing the electrostatic interaction of polyacrylic acid and chitosan;
crosslinking tannic acid with polyacrylic acid and chitosan in the interpenetrating network by using aluminum ions as a chelating agent; and
adding methylene acrylamide BIS, and bathing for 30-60 minutes at 60-80 ℃ to obtain the wound adhesive hydrogel material capable of repeatedly and specifically adhering biological soft tissues.
In the scheme, in the method, the hardness of the wound bonding hydrogel material is regulated and controlled by changing the content of the aluminum ion chelating agent; and/or by varying the tannin content to synthesize wound binding hydrogel materials with different binding strengths.
In order to achieve the above further object, the present invention also provides a use of the above wound-adhesive hydrogel material in the field of clinical medicine.
In the scheme, the wound bonding hydrogel material is used as a wound adhesive for surgical noninvasive suturing and daily wound treatment.
According to the technical scheme, the invention has the beneficial effects that:
1. the wound adhesive hydrogel material provided by the invention has excellent adhesive strength, can be adhered repeatedly under water, has ultrahigh adhesive efficiency, has the functions of underwater super adhesion and instant self-repairing, is antibacterial, is developed based on the inspiration of the pectinate tentacle for capturing the specificity of organisms and crustaceans in the sea, can be used for non-invasive suture of operations and daily wound treatment as a multifunctional wound adhesive tape, has the specificity adhesion of biological soft tissues under water, and is greatly convenient for clinical operation operations. Has ultrahigh bonding efficiency, saves operation time and is convenient for emergency.
2. Clinical experiments show that the adhesive strength of the wound adhesive hydrogel material provided by the invention to fresh pigskin in the air reaches 63.3kPa, repeated adhesion can be realized, the fault tolerance rate of operation is improved, and the pain of patients is relieved.
3. The wound adhesive hydrogel material provided by the invention has ultrahigh adhesion efficiency which is 6.33kPa/s, which is 10 times of that of the prior art, and can achieve stable adhesion within 10 seconds and improve the operation efficiency.
4. The wound adhesive hydrogel material provided by the invention has specific adhesion to biological soft tissues underwater, and the underwater adhesion strength reaches 18.1 kPa. The adhesive can not be stuck on gloves or surgical instruments during operation, blood at the wound does not need to be treated, and the adhesive can be quickly stuck, thereby being convenient for doctors to operate. Not limited to intra-operative use, outdoor sports injuries can also be used, with potential as a base for wearable devices.
5. The wound adhesive hydrogel material provided by the invention has excellent antibacterial performance. When escherichia coli and staphylococcus aureus S.aureus selected in the experiment are taken as pathogenic bacteria models, the hydrogel preparation shows remarkable antibacterial capacity.
6. The wound adhesive hydrogel material provided by the invention has good cell, animal biocompatibility and oxidation resistance. And has good self-healing property, excellent mechanical property and strong deformability.
7. The preparation method of the wound-adhesive hydrogel material provided by the invention is an antibacterial hydrogel preparation polymerized by adopting a one-pot method. The adhesive tape can be made into required size and shape according to the requirement, and can be directly covered on the wound.
8. The preparation method of the wound-adhesive hydrogel material provided by the invention is simple and convenient, and the wound-adhesive hydrogel material is convenient to store and carry after preparation.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic diagram of the synthesis of an antimicrobial and antioxidant wound-binding hydrogel material with ultra-high adhesion efficiency, underwater super adhesion and instantaneous self-healing capabilities, which is capable of being repeatedly bonded according to an embodiment of the present invention.
FIG. 2 is an infrared spectrum of a wound-adhesive hydrogel material according to an embodiment of the invention.
Fig. 3 is a scanning electron microscope image of a wound-adhesive hydrogel material in accordance with an embodiment of the invention.
FIG. 4 is a graph of a cyclic tensile test of a wound-adhesive hydrogel material according to an embodiment of the invention.
Fig. 5 is a graph illustrating an evaluation of the self-healing ability of a wound-adhesive hydrogel material according to an embodiment of the present invention.
Fig. 6 is a graph of data on the ability of a wound-adhesive hydrogel material to adhere in air in accordance with an embodiment of the invention.
Fig. 7 is a graph of data on the ability of a wound-adhesive hydrogel material to adhere underwater in accordance with an embodiment of the invention.
FIG. 8 is a graph illustrating the antimicrobial effect of a wound-adhesive hydrogel material in accordance with an embodiment of the invention.
FIG. 9 is a graph of in vitro L929 cytotoxicity assays of wound-adhesive hydrogel material leachate in accordance with embodiments of the present invention.
FIG. 10 is a graph showing the effect of a wound-adhesive hydrogel material according to an embodiment of the present invention in promoting wound healing in rats.
Fig. 11 is a graph of the potential of a wound-adhesive hydrogel material as a substrate for a wearable device, in accordance with an embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments and the accompanying drawings.
The embodiment of the invention relates to a wound bonding hydrogel material with the functions of biological soft tissue super-adhesion and antibiosis, a preparation method and application thereof. The wound adhesive hydrogel material with the biological soft tissue super-adhesion and antibacterial functions, provided by the embodiment of the invention, is inspirational by using a jellyfish tentacle, and can be used for designing a wound adhesive tape more suitable for operation. The stable bonding strength, the high bonding efficiency and the repeated bonding greatly save the operation time and the adhesive tape. The specific adhesion of the underwater adhesive to biological soft tissues is also convenient for doctors to operate, thereby further saving the operation time and reducing the pain of patients. Besides, the wearable device has the potential to be used as a base of the wearable device.
FIG. 1 is a schematic diagram of an antimicrobial and antioxidant wound-bonding hydrogel material with ultra-high adhesion efficiency, underwater super-adhesion and instantaneous self-healing functions, which can be repeatedly bonded according to an embodiment of the present invention. The main components of the wound adhesive hydrogel material comprise polyacrylic acid, chitosan, tannic acid and a chelating agent, wherein: polyacrylic acid and chitosan form an interpenetrating network through electrostatic interaction; tannic acid to enhance the viscosity and acidity of the wound-adhesive hydrogel; and the chelating agent is used for crosslinking the tannic acid with the polyacrylic acid and the chitosan in the interpenetrating network.
In one embodiment provided by the present invention, the polyacrylic acid serves as a primary network in the wound-adhesive hydrogel material, with a mass fraction of 19% in the wound-adhesive hydrogel material, for providing substantial mechanical strength and substantial tack.
In one embodiment provided by the present invention, the chitosan is present as a secondary network in the wound-adhesive hydrogel material, and the mass fraction in the wound-adhesive hydrogel material is 2-3%; the chitosan is protonated under the acidic condition of polyacrylic acid, inhibits the oxidation of tannic acid, reduces the inhibition effect of tannic acid, and acts with negatively charged groups on the surface of a tissue to improve the viscosity.
In one embodiment provided by the present invention, the tannic acid is used to assist in crosslinking and is present in the wound-binding hydrogel material at a weight fraction of 1-3%.
In one embodiment provided by the present invention, the chelating agent is Al-containing3+The inorganic salt of (1), the Al-containingMeterThe inorganic salt of (A) is Al (NO)3)3·9H2O or AlCl3And the like.
In one embodiment provided herein, the wound-adhesive hydrogel material further comprises: methylene acrylamide BIS, used to crosslink acrylic acid.
The wound adhesive hydrogel material with the biological soft tissue super-adhesion and antibacterial functions, which can be repeatedly used in the embodiment of the invention, has higher adhesive strength in air and water, can realize repeated adhesion, is stable in adhesion and is not influenced by acid and alkali. The bonding efficiency exceeds that of the current repeatedly-bondable hydrogel by 10 times, and is as high as 6.33 kPa/s. And it exhibits specific adhesion to biological soft tissue in water. The adhesive property is not affected by acid and alkali, and the wound-healing-promoting adhesive has antibacterial and healing-promoting effects.
The embodiment of the invention also provides a preparation method of the wound bonding hydrogel material, which adopts a one-pot polymerization method and specifically comprises the following steps:
forming an interpenetrating network by utilizing the electrostatic interaction of polyacrylic acid and chitosan;
crosslinking tannic acid with polyacrylic acid and chitosan in the interpenetrating network by using aluminum ions as a chelating agent; and
adding methylene acrylamide BIS, and bathing for 30-60 minutes at 60-80 ℃ to obtain the wound adhesive hydrogel material capable of repeatedly and specifically adhering biological soft tissues.
In the preparation method of the wound bonding hydrogel material, the hardness of the wound bonding hydrogel material is regulated and controlled by changing the content of the aluminum ion chelating agent; and/or by varying the tannin content to synthesize wound binding hydrogel materials with different binding strengths.
The embodiment of the invention also provides application of the wound bonding hydrogel material in the field of clinical medicine, and the wound bonding hydrogel material is used as a wound adhesive for surgical noninvasive suturing and daily wound treatment.
Example 1:
preparation method of wound-adhesive hydrogel material and test of performance of prepared wound-adhesive hydrogel material
The preparation method of the wound adhesive hydrogel material comprises the following steps: adding 3g of acrylic acid and 0.2-0.3g of chitosan into 10-13g (77%) of distilled water, fully stirring, then adding 0.06-0.08g of aluminum nitrate nonahydrate, adding 0.2-0.3g of tannic acid, adding 10mg of N, N.
Using infrared spectroscopy and scanning electron microscopyThe interaction of the above components of the wound-adhesive hydrogel material was analyzed under the microscope: the IR is shown in FIG. 2, which compares the IR spectra of different substances and illustrates the interaction between different substances in P-0.08Al-0.3C-0.3T (i.e., P represents polyacrylic acid, Al represents aluminum ion, C represents chitosan, and T represents tannic acid, according to the present invention). For P-0.3C-0.3T, compared with the material without tannic acid, at 3444cm-1and 3214cm-1The characteristic peak becomes larger due to phenolic hydroxyl group of tannic acid, which indicates the existence of tannic acid, and the peak becomes narrower and moves to the left, which indicates that phenolic hydroxyl group of tannic acid has interaction with chitosan. 2848cm-1and 2915cm-1The hydroxyl group on the carboxyl group of polyacrylic acid, and the sharp point indicates that tannic acid interacts with the hydroxyl group on polyacrylic acid. Carbonyl characteristic peak 1695cm of polyacrylic acid alone-1A red shift occurs indicating an interaction between polyacrylic acid and aluminum or chitosan. The invention is at 3434cm-1The peak value in the vicinity becomes large, which indicates that hydrogen bonds are formed between polyacrylic acid and chitosan. At 1317cm-1The unique peaks that appear are Tannic Acid (TA) and Al3+The interaction of (a). FIG. 3 is a scanning electron microscope image of the wound adhesive tape with a dense interstitial cross-section, with hydrogen bonding and electrostatic interactions greatly increasing the crosslink density.
The wound-adhesive hydrogel material was stretched using an STS10N universal testing machine, and self-healing ability was evaluated: tensile evaluation test temperature is 25 ℃, load force is 10N, and tensile rate is 100 mm/min. The test piece was evaluated for a length of 15mm, a diameter of 5mm, and 5-fold elongation by cyclic stretching. The resulting curve is shown in fig. 4. The self-healing ability evaluation experiment test parameters are the same as those of tensile evaluation, the sample strips are cut, the sections are aligned, the tensile test is carried out after the sections are kept for 30s, and a self-healing ability test chart is shown in fig. 5.
The wound-adhesive hydrogel material was evaluated for air and underwater adhesion using a STS10N model universal tester: the experimental test temperature was 25 ℃, the load force was 10N, and the tensile rate was 5 mm/min. The method adopts a fresh pigskin preparation procedure specified by a pharmaceutical industry standard YY/T0729.1-2009 to prepare pigskin with the length of 20mm, the width of 10mm and the thickness of 2 mm. In air or under water, using an area of10×10mm2The wound bonding hydrogel material is prepared by overlapping two pigskins, slightly pressing for 10s, then testing the underwater and water adhesion capability by using a shearing model, repeatedly measuring each group of samples for at least three times, and recording the stress-strain curve. As shown in fig. 6 (air), 7 (underwater). In fig. 6, the wound-adhesive hydrogel material was the most adhesive. FIG. 7 illustrates that the adhesive capacity of the wound to adhere hydrogel materials under water is directly proportional to the concentration of tannic acid. From fig. 6 and 7, the optimal formulation ratio of the wound-adhesive hydrogel material is 19% (mass fraction) of polyacrylic acid, 2% of tannic acid and 2% of chitosan. The adhesive strength in air can reach 63.3kPa, and the adhesive strength in water can reach 18.1 kPa.
The antibacterial performance of the wound adhesive hydrogel material was evaluated using escherichia coli (e.coli) and staphylococcus aureus (s.aureus) as pathogen models. Mixing 0.1g of hydrogel irradiated with ultraviolet for 30min with 10 g of hydrogel8The antibacterial performance was evaluated by plating after co-culturing for 24 hours in CFU/mL bacterial suspension. As shown in FIG. 8, the inhibition rates of Escherichia coli and Staphylococcus aureus were as high as 99.9% or more, respectively. The wound bonding hydrogel material is shown to have good antibacterial application prospect.
The biocompatibility of the material was evaluated according to the criteria for in vitro cytotoxicity in GB/T16886. The leach liquor was prepared according to the 0.1g/mL standard. 1g of wound adhesive tape was extracted in 10mL of RPMI 1640 medium at 37 ℃ for 24 h. Filtering with sterile filter membrane, and co-culturing with L929 fibroblast for 24h, 48h and 96 h. The Cell Count Kit-8 Kit is used for cytotoxicity analysis, and an enzyme-labeling instrument detects the light absorption value of the Kit at 450 nm. The blank group and the wound adhesive hydrogel material containing different components are designed for the detection of the experiment, and the cell experiment is carried out. The results are shown in FIG. 9, the activity of the cells treated by the wound-adhesive hydrogel material (P-0.08Al-0.3C-0.3T) leaching solution is above 70%, and the cells are obviously proliferated, and after being cultured for 96h, the activity is over 100% compared with that of the control group. The wound bonding hydrogel material is proved to have good biocompatibility and cell proliferation promoting effect.
Four experimental groups, namely a blank group, a suture group, a wound adhesive hydrogel material without tannic acid and a wound adhesive hydrogel material with tannic acid are designed in the experiment to evaluate the effect of the wound adhesive hydrogel material on promoting wound repair and healing. And 6 rats in each group are used as parallel controls, the rats are subjected to intraperitoneal injection anesthesia, back hairs are removed, four 2cm wounds are scratched on the back, after iodophor wiping, the four wounds are subjected to different treatment and are divided into blank groups and operation line suture groups, wound adhesive hydrogel materials without tannic acid are used for covering and adhering, and wound adhesive hydrogel materials with tannic acid are used for covering and adhering. Wound healing was observed on days 1, 3, 5, 7 and observed as h.e sections. The experimental results are shown in fig. 10, in which the wound-adhesive hydrogel material containing tannic acid in (a) has the most significant effect of promoting wound healing. (b) The slicing results showed that the gaps between epidermis and dermis of mice treated with the tannin-containing wound-adhesive hydrogel material were minimal, indicating that the wound-healing-promoting effect was most significant. (c) And (d) is a distance statistic result.
Example 2: the potential of the wound-adhesive hydrogel material as a substrate for wearable devices.
A sample strip 30mm long, 10mm wide and 2mm thick was cut and placed at the finger joint. The finger is externally connected with an electrochemical workstation, the finger is bent by 45 degrees and 90 degrees, and the change of an electric signal is observed, as shown in fig. 11, the wound healing can be promoted by weak current stimulation, which indicates that the wound bonding hydrogel material has the potential to be used as a wearable device substrate.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A wound-adhesive hydrogel material, the main components of which comprise polyacrylic acid, chitosan, tannic acid and a chelating agent, wherein:
polyacrylic acid and chitosan form an interpenetrating network through electrostatic interaction, the mass fraction of the polyacrylic acid in the wound-adhesive hydrogel material is 19%, and the mass fraction of the chitosan in the wound-adhesive hydrogel material is 2-3%;
tannin used for enhancing the viscosity and acidity of the wound bonding hydrogel, wherein the mass fraction of the tannin in the wound bonding hydrogel material is 1-3%;
the chelating agent is used for crosslinking the tannic acid with the polyacrylic acid and the chitosan in the interpenetrating network; the chelating agent is Al-containing3+The inorganic salt of (1), the Al-containing3+The inorganic salt of (A) is Al (NO)3)3∙9H2O or AlCl3Wherein Al is in the chelating agent3+The mass fraction of the organic silicon compound is 0.04% -0.055%;
wherein the wound-adhesive hydrogel material further comprises: methylene acrylamide BIS, used to crosslink acrylic acid.
2. A wound-adhesive hydrogel material according to claim 1, wherein the polyacrylic acid serves as a primary network in the wound-adhesive hydrogel material for providing substantial mechanical strength and substantial adhesion.
3. Wound-adhesive hydrogel material according to claim 1, characterized in that,
the chitosan acts as a secondary network in the wound-binding hydrogel material;
the chitosan is protonated under the acidic condition of polyacrylic acid, inhibits the oxidation of tannic acid, reduces the inhibition effect of tannic acid, and acts with negatively charged groups on the surface of a tissue to improve the viscosity.
4. The wound binding hydrogel material of claim 1, wherein the tannic acid is used to aid in crosslinking.
5. A method for preparing a wound-adhesive hydrogel material according to any one of claims 1 to 4, wherein the method employs a one-pot polymerization method, and specifically comprises:
forming an interpenetrating network by utilizing the electrostatic interaction of polyacrylic acid and chitosan;
crosslinking tannic acid with polyacrylic acid and chitosan in the interpenetrating network by using aluminum ions as a chelating agent; and
adding methylene acrylamide BIS, and bathing for 30-60 minutes at 60-80 ℃ to obtain a wound adhesive hydrogel material capable of repeatedly and specifically adhering biological soft tissues;
wherein the mass fraction of the polyacrylic acid in the wound-adhesive hydrogel material is 19%, the mass fraction of the chitosan in the wound-adhesive hydrogel material is 2-3%, the mass fraction of the tannic acid in the wound-adhesive hydrogel material is 1-3%, and the chelating agent is Al-containing3+The inorganic salt of (1), the Al-containing3+The inorganic salt of (A) is Al (NO)3)3∙9H2O or AlCl3。
6. The production method according to claim 5, wherein, in the method,
the hardness of the wound bonding hydrogel material is regulated and controlled by changing the content of the aluminum ion chelating agent; and/or
Wound-adhesive hydrogel materials with different adhesive strengths were synthesized by varying the content of tannic acid.
7. Use of a wound-adhesive hydrogel material according to any one of claims 1 to 4 for the preparation of a material for clinical medicine.
8. Use of the wound-adhesive hydrogel material according to claim 7 for the preparation of a wound adhesive for surgical non-invasive suturing and daily wound treatment.
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| CN112500585B (en) * | 2020-10-22 | 2022-11-15 | 湖北大学 | CS/TA/PAA-Al3+ composite ionic hydrogel and preparation method and application thereof |
| CN112354004B (en) * | 2020-10-29 | 2022-11-04 | 南方科技大学 | Medical hydrogel and preparation method and application thereof |
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| CN113637186B (en) * | 2021-08-27 | 2022-09-23 | 东南大学 | Preparation method of sustained adhesive hydrogel, hydrogel obtained by preparation method and application of hydrogel |
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