Disclosure of Invention
Aiming at the prior art, the invention provides a stem cell separation device, which improves the cell separation efficiency, is convenient to clean and disinfect, reduces the blockage of a cell screen and has high separation efficiency.
The technical scheme of the invention is realized as follows:
the utility model provides a stem cell separator, includes a jar body, sleeve, cell screen cloth and pushes away the water piston, the sleeve both ends are equipped with the opening and fix through the support the jar is internal, it is in to push away the water piston telescopic lower part motion, telescopic upper portion is equipped with the cell screen cloth, the sleeve with be equipped with first filter screen between the jar body, the cell screen cloth below be equipped with the second filter screen in the sleeve, the second filter screen is equipped with first through-hole, first through-hole is equipped with the first one-way baffle of opening downwards, telescopic lateral wall is equipped with the second through-hole, the second through-hole is equipped with the one-way baffle of outside open second, push away be equipped with in the water piston with the metal block, the jar is external to be equipped with the magnet, the magnet with the metal block inter attraction, the magnet drive the metal block along jar body up-and-down.
Furthermore, the height position of the second through hole is 3-8 cm smaller than that of the first through hole.
Further, a motor, a first driving lever and a second driving lever are arranged outside the tank body, one first driving lever and 2 second driving levers are arranged on an output shaft of the motor, the length of the first driving lever is larger than that of the second driving lever, a sliding ring is sleeved on the tank body and connected with the tank body through a spring, the magnet is fixed on the sliding ring, a bump is arranged on the sliding ring, and the first driving lever and the second driving lever are used for pushing the bump to slide upwards.
Further, push away water piston and include piston head and C shape ring, be equipped with in the piston head the metal block, the side of piston head is equipped with the annular, C shape ring is located in the annular.
Further, the C-shaped ring is made of a metal material.
Further, the cell screen is in a cone-shaped structure.
Furthermore, the first filter screen is of a conical structure, and the first through holes are formed in the bottom of the cell screen.
Further, the tank body is provided with a cover, and the cover is connected with the tank body through threads.
Further, the internal wall of jar is equipped with the draw-in groove, the support joint is in the draw-in groove.
The invention has the beneficial effects that: the cell soak solution forms internal circulation in the stem cell separation, and no new liquid needs to be additionally injected. In circulation, liquid passes through the cell screen in two directions of upward flow and downward flow, the downward flow promotes the separation of cells, the upward flow pushes the cells to be loose, the blockage of the cell screen is reduced, and the separation of stem cells is facilitated. In the circulation process, the first filter screen and the second filter screen both filter the cell soaking solution, and large-particle substances are prevented from returning to the upper part of the cell screen. During the downward reciprocating motion of the water pushing piston, the opening and closing of the first one-way baffle and the second one-way baffle can be automatically controlled, the cell loosening operation can be performed after every two times of stem cell filtration, the upward flowing liquid is filtered every time, and the liquid flowing back to the upper part of the cell screen is also filtered. When the water pushing piston moves downwards, the stem cell separation can be promoted, and the cell soaking solution is pushed back to the upper part of the cell screen. When the water pushing piston moves upwards, the cell soaking liquid in the sleeve can be discharged or the cells can be loosened. The water pushing piston is driven by the magnet to move up and down outside the tank body, the tank body and parts in the tank body can be disinfected at high temperature, and the driving parts are prevented from polluting the environment in the tank body.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only preferred embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a schematic cross-sectional view of a stem cell separation device according to the present invention;
FIG. 2 is a schematic structural view of a motor, a first shift lever and a second shift lever according to the present invention;
in the figure, 1 a tank body, 2 sleeves, 3 cell screens, 4 water pushing pistons, 5 supports, 6 first filter screens, 7 second filter screens, 8 first through holes, 9 first one-way baffles, 10 second through holes, 11 second one-way baffles, 12 metal blocks, 13 magnets, 14 motors, 15 first deflector rods, 16 second deflector rods, 17 slip rings, 18 springs, 19C-shaped rings, 20 piston heads, 21 annular grooves, 22 covers, 23 clamping grooves and 24 convex blocks.
Detailed Description
In order to better understand the technical content of the invention, specific embodiments are provided below, and the invention is further described with reference to the accompanying drawings.
Referring to fig. 1-2, a stem cell separation device comprises a tank body 1, a sleeve 2, a cell screen 3 and a water pushing piston 4, wherein openings are arranged at two ends of the sleeve 2 and fixed in the tank body 1 through a support 5, the water pushing piston 4 moves at the lower part of the sleeve 2, the cell screen 3 is arranged at the upper part of the sleeve 2, a first filter screen 6 is arranged between the sleeve 2 and the tank body 1, a second filter screen 7 is arranged in the sleeve 2 below the cell screen 3, the second filter screen 7 is provided with a first through hole 8, the first through hole 8 is provided with a first one-way baffle 9 which is opened downwards, a second through hole 10 is arranged on the side wall of the sleeve 2, the second through hole 10 is provided with a second one-way baffle 11 which is opened outwards, a metal block 12 is arranged in the water pushing piston 4, a magnet 13 is arranged outside the tank body 1, and the magnet 13 and the metal block 12 attract each other, the magnet 13 drives the metal block 12 to move up and down along the can body 1.
Pouring cell sample liquid to be separated into a sleeve 2, arranging a cell screen 3 at the upper part in the sleeve 2, and separating stem cells by using the cell screen 3. The upper and lower both ends of sleeve 2 are equipped with the opening to in jar body 1 was located to sleeve 2, cell soak can flow in from the upper end of sleeve 2, flows out from the lower extreme. The lower part of the sleeve 2 is provided with a water pushing piston 4, and when the water pushing piston 4 moves downwards, the cell soaking liquid is pumped into the sleeve 2. The lateral wall of sleeve 2 is equipped with second through-hole 10, and second through-hole 10 is equipped with outside open-ended second one-way baffle 11, and is optional, and second one-way baffle 11 has elasticity, and one side is connected with sleeve 2, the cell screen cloth 3 below be equipped with second filter screen 7 in the sleeve 2, second filter screen 7 is equipped with first through-hole 8, first through-hole 8 is equipped with first one-way baffle 9 of opening downwards, and is optional, and first one-way baffle 9 has elasticity, and one side is connected with second filter screen 7. When the push piston 4 moves downward below the second through hole 10: the second one-way baffle 11 covers the second through hole 10 under the action of water flow, so that the second through hole 10 is sealed, the cell soaking solution cannot flow into the sleeve 2 from the second through hole 10, meanwhile, the first one-way baffle 9 moves downwards, the first through hole 8 is opened, so that the cell soaking solution flows from the upper part to the lower part of the sleeve 2, the separation of the cell screen 3 on stem cells is promoted, the cell soaking solution in the sleeve 2 flows into the gap between the sleeve 2 and the tank body 1 from the bottom of the sleeve 2, and then upwards overflows back to the upper part of the sleeve 2 through the first filter screen 6, and due to the filtration of the first filter screen 6, the aperture of the first filter screen 6 and the aperture of the second filter screen 7 are smaller than that of the cell screen 3, and large granular substances and cells cannot return to the top of the sleeve 2. When the water pushing piston 4 moves upwards under the second through hole 10, the first one-way baffle 9 moves upwards to block the first through hole 8, the first through hole 8 is closed, and simultaneously, the second one-way baffle 11 opens outwards, so that the cell soaking solution flows out of the sleeve 2 from the second through hole 10. When pushing away water piston 4 and continuing upward movement after upwards moving to second through-hole 10, second through-hole 10 is blocked or is located and pushes away water piston 4 below by pushing away water piston 4, first through-hole 8 and second through-hole 10 are all closed, the cell soak squeezes second filter screen 7 upward flow, because large granule material and cell are kept off by the check under the filtering action of filter screen, the cell soak flows upward and strikes cell screen 3, with the cell pine of cell screen 3 top scatter, be convenient for next time cell screen 3 separates the stem cell. The water pushing piston 4 moves downwards after reaching the top extreme position, when the water pushing piston moves downwards above the second through hole 10, the first one-way baffle 9 moves downwards to open the first through hole 8, and the cell soak solution flows downwards to promote the cell screen 3 to separate the cells. Push away in the water piston 4 be equipped with metal block 12, jar body 1 is equipped with magnet 13 outward, magnet 13 with metal block 12 inter attraction, magnet 13 drive metal block 12 along jar body 1 up-and-down motion, magnet 13 are located outside jar body 1, and rethread magnetic field drive pushes away water piston 4 up-and-down motion, avoids actuating mechanism contact jar body 1 inside, and jar body 1, sleeve 2, parts such as the water piston 4 that push away all can carry out high temperature sterilization, ensure that stem cell separation process is not polluted, the device is clean and disinfection easily. The magnet 13 reciprocates 2 times below the second through hole 10, and then reciprocates 1 time above the second through hole 10.
Preferably, the height position of the second through hole 10 is 3-8 cm smaller than that of the first through hole 8. The water pushing piston 4 has a certain moving space above the second through hole 10, so that water is pushed conveniently to loosen cells.
Optionally, a motor 14, a first driving lever 15 and a second driving lever 16 are arranged outside the tank body 1, one first driving lever 15 and 2 second driving levers 16 are arranged on an output shaft of the motor 14, the length of the first driving lever 15 is greater than that of the second driving lever 16, a slip ring 17 is sleeved on the tank body 1, the slip ring 17 is connected with the tank body 1 through a spring 18, the magnet 13 is fixed on the slip ring 17, the slip ring 17 is provided with a convex block 24, and the first driving lever 15 and the second driving lever 16 are used for pushing the convex block 24 to slide upwards. The first shift lever 15 and the second shift lever 16 are perpendicular to an output shaft of the motor 14, and when the motor 14 rotates, the first shift lever 15 and the second shift lever 16 rotate, so that the convex block 24 is pushed to move upwards, and the slip ring 17 is driven to move upwards. Since the length of the first lever 15 is greater than the length of the second lever 16, the distance that the first lever 15 pushes the slip ring 17 upward is greater than the distance that the second lever 16 pushes the slip ring 17 upward, thereby ensuring that the magnet 13 reciprocates 2 times below the second through hole 10 and then reciprocates 1 time above the second through hole 10. The slip ring 17 is sleeved on the tank body 1, the slip ring 17 can move up and down, the magnet 13 can move up and down along with the slip ring 17, the deviation of the magnet 13 is avoided, and the components such as the tank body 1 can be sterilized after the magnet 13 is taken down. Preferably, the motor 14 outside the tank body 1 is fixed on the base, and the tank body 1 is clamped with the base. After the pushing force of the cam 17 is lost, the sliding ring 17 moves downwards to be reset under the pulling force of the spring 18.
Optionally, the water piston 4 includes a piston head 20 and a plurality of C-shaped rings 19, the metal block 12 is disposed in the piston head 20, a ring groove 21 is disposed on a side surface of the piston head 20, and the C-shaped rings 19 are disposed in the ring groove 21. The C-shaped ring 19 is made of elastic material, and the annular openings are staggered, so that the sealing performance of the water pushing piston 4 is improved, and the water pushing capacity is provided.
Preferably, the C-shaped ring 19 is made of a metal material. The magnetic sleeve is made of magnetizable metal material, so that the magnetic sleeve is convenient to be attached to the sleeve 2 under the action of the magnet 13, and the sealing effect is improved.
Specifically, the cell screen 3 has a conical structure. The surface area of the cell screen 3 is increased, and the cell separation effect is improved.
Specifically, the first filter screen 6 is a conical structure, and the first through hole 8 is formed in the bottom of the cell screen 3. The area of the filter screen is increased, and the filtering effect is improved.
Specifically, the can body 1 is provided with a cover 22, and the cover 22 is connected with the can body 1 through threads. The tank body 1 is covered by a cover 22 to avoid overflowing of the cell soaking solution in the separation process.
Optionally, the inner wall of the tank body 1 is provided with a clamping groove 23, and the bracket 5 is clamped in the clamping groove 23. The bracket 5 is clamped in the tube body, and can be detached during cleaning and disinfection, thereby being convenient for operation.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.