CN111498989A - Preparation method of facultative anaerobe filler for in-situ purification of domestic sewage - Google Patents
Preparation method of facultative anaerobe filler for in-situ purification of domestic sewage Download PDFInfo
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- CN111498989A CN111498989A CN202010298995.XA CN202010298995A CN111498989A CN 111498989 A CN111498989 A CN 111498989A CN 202010298995 A CN202010298995 A CN 202010298995A CN 111498989 A CN111498989 A CN 111498989A
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- filler
- domestic sewage
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- facultative anaerobic
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2806—Anaerobic processes using solid supports for microorganisms
Abstract
A preparation method of facultative anaerobic microbial filler for in-situ purification of domestic sewage is characterized in that facultative anaerobic microbial bacteria capable of treating the domestic sewage can be constructed by the method, the bacteria are loaded on a carrier with large surface area, multiple holes and rough surface, efficient loading of microbial strains is realized, and the facultative anaerobic microbial filler capable of treating the domestic sewage is obtained by a freeze drying technology. The invention has the advantages that: the technology of the invention can quickly realize the fixation of the facultative anaerobe for treating the domestic sewage on the carrier, and the effective viable count is higher than 1010cfu/g. The facultative anaerobe filler constructed by the invention has the characteristics of high porosity, large specific surface area, rich functional groups (hydroxyl and amino), high effective viable count and the like. The filler constructed by the invention can obviously improve the facultative anaerobic treatment efficiency and accelerate the removal of pollutants.
Description
Technical Field
The invention relates to a domestic sewage treatment technology, in particular to a preparation method of a facultative anaerobe filler for in-situ purification of domestic sewage.
Background
With the development of the times and the improvement of the living standard of people, the pace of city construction is accelerated, the increasing consumption demand of people and the desire of people for beautiful cities are stronger and stronger, and the construction of rural sewage infrastructure is relatively lagged behind, so that rural sewage is discharged into river channels, earth surfaces and the like, mosquitoes and flies are bred while the environment is seriously polluted, foul gas is generated, and the personal health is threatened. Rural sewage treatment is an important component of the sewage treatment industry in China, only sewage generated in the production and living processes is treated in relative cities and towns, and the rural sewage treatment is accompanied with environmental improvement and protection contents such as rural drinking water source area protection, rural domestic garbage treatment, livestock and poultry breeding waste resource utilization, pollution prevention and control and the like. However, the coverage rate of rural sewage treatment in China is not high, so how to efficiently treat rural sewage according to local conditions becomes one of the primary tasks of current social development.
At present, the rural sewage treatment mostly adopts centralized treatment, and the technical method comprises the following steps: an artificial wetland treatment system and an underground soil infiltration and purification system based on the natural ecological principle, an aerobic biological treatment system and an anaerobic biological treatment system based on biological treatment. However, in rural areas with dispersed residents and complex landforms, the cost of infrastructure construction (pipe network construction) in the early stage of centralized treatment is extremely high, and the treatment of rural sewage treatment is severely restricted. Meanwhile, the efficiency of the micro-scale dispersed microorganism strains becomes one of the main problems restricting the rural sewage treatment. Therefore, in order to solve the problem in the existing remote rural sewage treatment and disposal process, the invention develops the preparation method of the high-efficiency facultative anaerobe filler so as to improve the sewage treatment efficiency.
Disclosure of Invention
The invention aims to overcome the defects of the existing rural domestic sewage treatment and provides the preparation method of the facultative anaerobe filler for the in-situ purification of the domestic sewage.
The technical scheme of the invention is as follows:
a preparation method of a facultative anaerobic bacteria filler for in-situ purification of domestic sewage is characterized in that the facultative anaerobic microorganism filler is formed by natural growth, loading and cultivation on the filler, and the steps are as follows:
1) introducing domestic sewage into a facultative anaerobic tank provided with a micro-aeration device, and maintaining dissolved oxygen at 0.1-1.0 mg/l;
2) placing the porous chitosan @ lignin filler rich in hydroxyl and amino in the facultative anaerobic tank, immersing the filler in domestic sewage, and maintaining the water temperature at 10-25 ℃;
3) according to the water circulation flow (ml/min) of 1-2% of the capacity of the facultative anaerobic tank, carrying out circulation treatment on the domestic sewage in the facultative anaerobic tank;
4) the system is continuously operated, and the number of facultative anaerobes capable of treating the domestic sewage on the filler is gradually increased after 3-5 days;
5) the continuous operation is carried out for 6 to 12 days, and the density of the facultative anaerobes distributed on the filler reaches 1.0 × 1010cfu/g；
6) And taking out the filler, and placing the filler in a freeze dryer for freeze drying, thereby obtaining the required facultative anaerobic microbial filler capable of treating domestic sewage.
Wherein the domestic sewage is wastewater generated in the life process of residents.
The porous chitosan @ lignin is a porous material with a core and chitosan wrapped outside the core.
The invention has the advantages and beneficial effects that:
the facultative anaerobe filler constructed by the invention has the characteristics of high porosity, large specific surface area, rich functional groups (hydroxyl and amino), high effective viable count and the like; meanwhile, the surface of the carrier is rough, so that the growth of microorganisms on the carrier is facilitated, and the immobilization of the microorganisms is realized. The loaded facultative anaerobic microorganisms are microorganisms capable of effectively treating domestic sewage, are high in treatment efficiency and accelerate removal of pollutants.
Detailed Description
The present invention is further described in the following examples, but the technical content described in the examples is illustrative and not restrictive, and the scope of the present invention should not be limited thereby.
Example 1:
a preparation method of a facultative anaerobic bacteria filler for in-situ purification of domestic sewage is characterized in that the facultative anaerobic microorganism filler is formed by natural growth, loading and cultivation on the filler, and the steps are as follows:
1) introducing domestic sewage into a facultative anaerobic tank provided with a micro-aeration device, and maintaining dissolved oxygen at 0.1 mg/l;
2) placing the porous chitosan @ lignin filler rich in hydroxyl and amino in the facultative anaerobic tank, immersing the filler in domestic sewage, and maintaining the water temperature at 15 ℃;
3) according to the water circulation flow (ml/min) of 1% of the capacity of the facultative anaerobic tank, carrying out circulation treatment on the domestic sewage in the facultative anaerobic tank;
4) the system is continuously operated, and the number of facultative anaerobes capable of treating the domestic sewage is gradually increased after 4 days of filling;
5) the continuous operation is carried out for 8 days, and the density of the facultative anaerobes distributed on the filler reaches 1.0 × 1010cfu/g；
6) And taking out the filler, and placing the filler in a freeze dryer for freeze drying, thereby obtaining the required facultative anaerobic microbial filler capable of treating domestic sewage.
Wherein the domestic sewage is wastewater generated in the life process of residents.
The porous chitosan @ lignin is a porous material with a core and chitosan wrapped outside the core.
Example 2:
a preparation method of a facultative anaerobic bacteria filler for in-situ purification of domestic sewage is characterized in that the facultative anaerobic microorganism filler is formed by natural growth, loading and cultivation on the filler, and the steps are as follows:
1) introducing domestic sewage into a facultative anaerobic tank provided with a micro-aeration device, and maintaining dissolved oxygen at 0.3 mg/l;
2) placing the porous chitosan @ lignin filler rich in hydroxyl and amino in the facultative anaerobic tank, immersing the filler in domestic sewage, and maintaining the water temperature at 20 ℃;
3) according to the water circulation flow (ml/min) of 1.5 percent of the capacity of the facultative anaerobic tank, the domestic sewage in the facultative anaerobic tank is circularly treated;
4) the system is continuously operated, and the number of facultative anaerobes capable of treating the domestic sewage is gradually increased after 5 days of filling;
5) the continuous operation is carried out for 10 days, and the density of the facultative anaerobes distributed on the filler reaches 1.0 × 1010cfu/g；
6) And taking out the filler, and placing the filler in a freeze dryer for freeze drying, thereby obtaining the required facultative anaerobic microbial filler capable of treating domestic sewage.
Wherein the domestic sewage is wastewater generated in the life process of residents.
The porous chitosan @ lignin is a porous material with a core and chitosan wrapped outside the core.
Example 3:
a preparation method of a facultative anaerobic bacteria filler for in-situ purification of domestic sewage is characterized in that the facultative anaerobic microorganism filler is formed by natural growth, loading and cultivation on the filler, and the steps are as follows:
1) introducing the domestic sewage into a facultative anaerobic tank provided with a micro-aeration device, and maintaining the dissolved oxygen at 0.4 mg/l;
2) placing the porous chitosan @ lignin filler rich in hydroxyl and amino in the facultative anaerobic tank, immersing the filler in domestic sewage, and maintaining the water temperature at 25 ℃;
3) performing circulating treatment on the domestic sewage in the facultative anaerobic tank according to the water circulation flow (ml/min) of 2 percent of the capacity of the facultative anaerobic tank;
4) the system is continuously operated, and the number of facultative anaerobes capable of treating the domestic sewage is gradually increased after 5 days of filling;
5) the continuous operation is carried out for 11 days, and the density of the facultative anaerobes distributed on the filler reaches 1.0 × 1010cfu/g；
6) And taking out the filler, and placing the filler in a freeze dryer for freeze drying, thereby obtaining the required facultative anaerobic microbial filler capable of treating domestic sewage.
Wherein the domestic sewage is wastewater generated in the life process of residents.
The porous chitosan @ lignin is a porous material with a core and chitosan wrapped outside the core.
Claims (3)
1. A preparation method of a facultative anaerobic bacteria filler for in-situ purification of domestic sewage is characterized in that the facultative anaerobic microorganism filler is formed by natural growth, loading and cultivation on the filler, and the steps are as follows:
1) introducing domestic sewage into a facultative anaerobic tank provided with a micro-aeration device, and maintaining dissolved oxygen at 0.1-1.0 mg/l;
2) placing the porous chitosan @ lignin filler rich in hydroxyl and amino in the facultative anaerobic tank, immersing the filler in domestic sewage, and maintaining the water temperature at 10-25 ℃;
3) according to the water circulation flow (ml/min) of 1-2% of the capacity of the facultative anaerobic tank, carrying out circulation treatment on the domestic sewage in the facultative anaerobic tank;
4) the system is continuously operated, and the number of facultative anaerobes capable of treating the domestic sewage on the filler is gradually increased after 3-5 days;
5) the continuous operation is carried out for 6 to 12 days, and the density of the facultative anaerobes distributed on the filler reaches 1.0 × 1010cfu/g；
6) And taking out the filler, and placing the filler in a freeze dryer for freeze drying, thereby obtaining the required facultative anaerobic microbial filler capable of treating domestic sewage.
2. The preparation method of the facultative anaerobe filler for in-situ purification of domestic sewage according to claim 1, wherein the facultative anaerobe filler comprises: the domestic sewage is wastewater generated in the life process of residents.
3. The preparation method of the facultative anaerobe filler for in-situ purification of domestic sewage according to claim 1, wherein the facultative anaerobe filler comprises: the porous chitosan @ lignin is a porous material with a core coated with chitosan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010298995.XA CN111498989A (en) | 2020-04-16 | 2020-04-16 | Preparation method of facultative anaerobe filler for in-situ purification of domestic sewage |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010298995.XA CN111498989A (en) | 2020-04-16 | 2020-04-16 | Preparation method of facultative anaerobe filler for in-situ purification of domestic sewage |
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| CN111498989A true CN111498989A (en) | 2020-08-07 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214106B (en) * | 2013-05-22 | 2014-03-26 | 南京林业大学 | Method for biologically degrading organic wastewater by utilizing microbe |
| CN104445597A (en) * | 2014-11-24 | 2015-03-25 | 浙江大学 | Method for strengthening raw water biological pretreatment biological membrane enrichment and operating performance stability |
| CN104671409A (en) * | 2014-12-31 | 2015-06-03 | 浙江至美环境科技有限公司 | Method for treating sewage by using biological fluidized bed |
| CN106365294A (en) * | 2016-08-25 | 2017-02-01 | 李勇锋 | Microorganism carrier and preparation method of same |
| WO2018007429A1 (en) * | 2016-07-05 | 2018-01-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Lignin based composite material and method for its production |
-
2020
- 2020-04-16 CN CN202010298995.XA patent/CN111498989A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214106B (en) * | 2013-05-22 | 2014-03-26 | 南京林业大学 | Method for biologically degrading organic wastewater by utilizing microbe |
| CN104445597A (en) * | 2014-11-24 | 2015-03-25 | 浙江大学 | Method for strengthening raw water biological pretreatment biological membrane enrichment and operating performance stability |
| CN104671409A (en) * | 2014-12-31 | 2015-06-03 | 浙江至美环境科技有限公司 | Method for treating sewage by using biological fluidized bed |
| WO2018007429A1 (en) * | 2016-07-05 | 2018-01-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Lignin based composite material and method for its production |
| CN106365294A (en) * | 2016-08-25 | 2017-02-01 | 李勇锋 | Microorganism carrier and preparation method of same |
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Application publication date: 20200807 |