CN111495445B

CN111495445B - Test tray and test system

Info

Publication number: CN111495445B
Application number: CN202010131019.5A
Authority: CN
Inventors: 陈跃东; 陈杰; 顾志鹏; 刘仁源
Original assignee: North South Brothers Pharmaceutical Investment Co ltd
Current assignee: North South Brothers Pharmaceutical Investment Co ltd
Priority date: 2020-02-28
Filing date: 2020-02-28
Publication date: 2022-01-14
Anticipated expiration: 2040-02-28
Also published as: CN111495445A

Abstract

The invention provides a test disc and a test system, which relate to the technical field of microfluidics and comprise: the tray body is provided with at least one group of detection structures; the detection structure comprises at least one liquid storage tank, at least one waste liquid tank and at least one receiving tank; each waste liquid tank is communicated with at least one liquid storage tank in a matching way, and each liquid storage tank is communicated with at least one receiving tank in a matching way; the waste liquid tank is communicated with a waste liquid outlet of the liquid storage tank, and the maximum diameters of the waste liquid tank and the waste liquid outlet on the tray body are smaller than the maximum diameter of the liquid storage tank on the tray body; the first valve is arranged between the liquid storage tank and the receiving groove, and the maximum diameter of the liquid storage tank on the tray body is smaller than the maximum diameter of the receiving groove on the tray body; the water absorbing material is disposed in the waste liquid tank. In the technical scheme, the relative radial position relation of the liquid storage tank and the waste liquid tank on the tray body is adjusted, and the water absorption material is utilized to ensure that the waste liquid cannot flow back, so that the design of the test tray with higher integration level and smaller diameter size is facilitated.

Description

Test tray and test system

Technical Field

The invention relates to the technical field of microfluidics, in particular to a test disc and a test system.

Background

In the technical field of microfluidics, the test disc has the advantages of good integration performance and simple control, so that the test disc can be widely applied to the field of POCT (point of care testing) including biochemistry, immunity and molecular diagnosis. However, with the development of the industry, the miniaturization of the diameter size of the test disc faces the bottleneck, and the diameter size of the test disc in the prior art is generally large, so that the test disc is not easy to carry and cannot meet the existing use requirements.

Disclosure of Invention

The invention aims to provide a test disc and a test system, which aim to solve the technical problem that the size of the test disc is larger in the prior art.

The invention provides a test disc, comprising:

the tray body is provided with at least one group of detection structures;

the detection structure comprises at least one liquid storage tank, at least one waste liquid tank and at least one receiving tank; each waste liquid groove is communicated with at least one liquid storage groove in a matching way, and each liquid storage groove is communicated with at least one receiving groove in a matching way;

the waste liquid tank is communicated with a waste liquid outlet of the liquid storage tank, and the maximum diameters of the waste liquid tank and the waste liquid outlet on the tray body are smaller than the maximum diameter of the liquid storage tank on the tray body;

a first valve mounted between the reservoir and the receiving tank, a maximum diameter of the reservoir on the tray body being smaller than a maximum diameter of the receiving tank on the tray body;

a water absorbent material disposed within the waste tank.

Further, the test tray further includes:

a second valve mounted between the reservoir and the waste reservoir.

Further, the test tray further includes:

a first passage through which the reservoir and the receiving groove communicate, the maximum diameter of the reservoir on the tray body being smaller than the minimum diameter of the receiving groove on the tray body;

the first valve is mounted within the first passage.

Further, the test tray further includes:

the waste liquid tank and the liquid storage tank are communicated through the second channel;

the second channel is communicated with a waste liquid inlet of the waste liquid tank, and the maximum diameter of the waste liquid inlet on the tray body is equal to the maximum diameter of the waste liquid tank on the tray body; and/or the second channel is communicated with the waste liquid outlet, and the waste liquid outlet is positioned between the maximum diameter and the minimum diameter of the liquid storage tank on the tray body.

Further, the test tray further includes:

and the quality control reagent is arranged on the water absorbing material.

Further, the test tray further includes:

the cover body is covered with the disc surface of the disc body relatively.

Furthermore, at least one through hole corresponding to the detection structure is formed in the cover body.

Further, the waste liquid tank comprises a first waste liquid tank, the liquid storage tank comprises a first liquid storage tank and a second liquid storage tank, and the receiving tank comprises a first receiving tank and a second receiving tank; the first waste liquid tank is communicated with the first liquid storage tank and the second liquid storage tank at the same time, the first liquid storage tank is communicated with the first receiving groove, and the second liquid storage tank is communicated with the second receiving groove.

Further, the waste liquid tank further comprises a second waste liquid tank, the liquid storage tank further comprises a third liquid storage tank, and the receiving tank further comprises a third receiving tank; the second waste liquid tank is communicated with the third liquid storage tank, and the third liquid storage tank is communicated with the third receiving groove.

Further, the test tray further includes:

a fluid passage through which the first receiving groove, the second receiving groove, and the third receiving groove communicate in order.

Further, the test tray further includes:

a sample introduction well, a sample processing channel, and an aspiration channel;

the sample processing channel is in communication with the sample introduction well, one end of the siphon channel is in communication with the sample introduction well, and the other end of the siphon channel is in communication with the first receiving well.

Furthermore, the detection structure is the multiunit, the multiunit the detection structure use the axis of disk body is the axle and is the circumference array and arranges.

Further, the first valve and/or the second valve is a phase change valve.

The invention also provides a test system, which comprises the test disc; further comprising:

a centrifuge device, the test tray being mounted on a driving part of the centrifuge device;

the identification part of the image identification device is arranged corresponding to the waste liquid groove of the test disc, and the control part of the magnetic bead transfer device is arranged corresponding to the receiving groove.

In the technical scheme, the relative radial position relation of the liquid storage tank and the waste liquid tank on the tray body is adjusted by the test tray, so that the waste liquid tank is arranged at the position which is radially inward relative to the liquid storage tank, the problem that the diameter size of the tray body is limited by the waste liquid tank is solved, and meanwhile, the waste liquid cannot flow back to the liquid storage tank from the waste liquid tank by utilizing the water absorbing material, so that the test tray which is higher in integration level and smaller in diameter size is favorably designed.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a plan view of a prior art test tray;

FIG. 2 is a perspective view of a tray provided in accordance with one embodiment of the present invention;

FIG. 3 is a plan view of a tray provided in accordance with one embodiment of the present invention;

FIG. 4 is an assembled perspective view of a first valve provided in accordance with one embodiment of the present invention;

FIG. 5 is an assembled plan view of a first valve provided in accordance with one embodiment of the present invention;

FIG. 6 is an exploded view of a test tray provided in accordance with one embodiment of the present invention;

FIG. 7 is a perspective view of a tray provided in accordance with another embodiment of the present invention;

FIG. 8 is a plan view of a tray provided in accordance with another embodiment of the present invention;

FIG. 9 is an exploded view of a test tray provided in accordance with another embodiment of the present invention;

FIG. 10 is a perspective view of a through hole provided in accordance with one embodiment of the present invention;

FIG. 11 is a fill plan view of a liquid reagent provided in accordance with one embodiment of the present invention;

FIG. 12 is a filled perspective view of a liquid reagent provided in accordance with one embodiment of the present invention;

FIG. 13 is a quantitative plan view of a liquid reagent provided in accordance with one embodiment of the present invention;

FIG. 14 is a perspective view of a quantitative liquid reagent provided in accordance with an embodiment of the present invention;

FIG. 15 is a receiving plan view of a liquid reagent provided by one embodiment of the present invention;

FIG. 16 is a receiving perspective view of a liquid reagent provided by one embodiment of the present invention;

FIG. 17 is a plan view of a tray provided in accordance with yet another embodiment of the present invention;

FIG. 18 is a perspective view of a tray provided in accordance with yet another embodiment of the present invention;

FIG. 19 is an exploded view of a test tray provided in accordance with yet another embodiment of the present invention;

fig. 20 is a schematic plan view of a through hole according to an embodiment of the present invention.

Reference numerals:

1. a tray body; 2. a cover body; 3. detecting the structure; 4. a liquid reagent;

11. a dish surface; 12. a shaft hole; 21. a through hole;

31. a liquid storage tank; 32. a waste liquid tank; 33. a receiving groove; 34. a first valve; 35. a water-absorbing material; 36. a second valve; 37. a first channel; 38. a second channel;

311. a waste liquid outlet; 312. a first reservoir; 313. a second reservoir; 314. a third reservoir;

321. a waste liquid inlet; 322. a first waste liquid tank; 323. a second waste liquid tank;

331. a first receiving groove; 332. a second receiving groove; 333. a third receiving groove;

334. a fluid channel; 335. a sample introduction well; 336. a sample processing channel; 337. an siphon channel.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As a result of research on the detecting structure 3 of the conventional test tray, it was found that the quantitative design structure of the test tray in the prior art is one of the reasons that the miniaturization (i.e., diameter size reduction) of the test tray size is limited. Specifically, the quantitative design structure of the conventional test tray is generally constituted by the waste liquid tank 32 and the reservoir 31. In order to ensure that the excessive waste liquid in the liquid storage tank 31 can smoothly enter the waste liquid tank 32 under the centrifugal action and still can be kept in the waste liquid tank 32 without flowing back to the liquid storage tank 31 in the continuous process of the centrifugal motion, the waste liquid tank 32 on the test tray is arranged at a position radially outward relative to the liquid storage tank 31, that is, at least the maximum diameter of the waste liquid tank 32 on the test tray is required to be larger than the maximum diameter of the liquid storage tank 31 on the test tray, such as the structure shown in fig. 1, so as to ensure that the waste liquid entering the waste liquid tank 32 can be kept in the tank structure part radially outward in the waste liquid tank 32 under the centrifugal action and cannot flow back.

It can be seen that, as shown in fig. 1, the position of the waste liquid tank 32 is closer to the edge of the tray body 1 (i.e. the radially outward position) relative to the liquid storage tank 31, so that the diameter size of the conventional test tray is determined by the radial position of the waste liquid tank 32 on the test tray, and this structural design can ensure that the excessive waste liquid in the liquid storage tank 31 cannot flow back from the waste liquid tank 32, but the diameter size of the test tray is continuously increased, i.e. the smaller the radial position of the waste liquid tank 32 on the test tray, the larger the minimum diameter size of the test tray is, and there is a desire to make the size of the test tray as small as possible.

Therefore, the present application provides a technical solution that, on the basis of ensuring that the waste liquid in the waste liquid tank 32 does not flow back, the restriction of the position of the waste liquid tank 32 on the miniaturization of the diameter size of the test tray is eliminated, which is beneficial to designing the test tray with higher integration level and smaller diameter size.

As shown in fig. 2 to 5, the present embodiment provides a test tray, including:

the detection device comprises a tray body 1, wherein at least one group of detection structures 3 are arranged on the tray surface 11 of the tray body 1;

the detection structure 3 comprises at least one reservoir 31, at least one waste reservoir 32 and at least one receiving tank 33; each waste liquid tank 32 is communicated with at least one liquid storage tank 31 in a matching way, and each liquid storage tank 31 is communicated with at least one receiving groove 33 in a matching way;

the waste liquid tank 32 is communicated with a waste liquid outlet 311 of the liquid storage tank 31, and the maximum diameters of the waste liquid tank 32 and the waste liquid outlet 311 on the tray body 1 are both smaller than the maximum diameter of the liquid storage tank 31 on the tray body 1;

a first valve 34, the first valve 34 being installed between the reservoir tank 31 and the receiving groove 33, a maximum diameter of the reservoir tank 31 on the tray body 1 being smaller than a maximum diameter of the receiving groove 33 on the tray body 1;

a water absorbing material 35, said water absorbing material 35 being disposed in said waste liquid tank 32.

With continued reference to fig. 2 and 3, in order to eliminate the restriction of the radial position of the waste liquid tank 32 on the diameter size of the tray body 1, the test tray provided by the present application adjusts the relative radial positional relationship of the liquid storage tank 31 and the waste liquid tank 32 on the tray body 1, and defines that the maximum diameter of the waste liquid tank 32 on the tray body 1 is smaller than the maximum diameter of the liquid storage tank 31 on the tray body 1, i.e., the waste liquid tank 32 is disposed at a radially inward position relative to the liquid storage tank 31 in the radial direction of the tray body 1. Therefore, when the maximum diameter of the waste liquid tank 32 is smaller than the maximum diameter of the liquid storage tank 31, the waste liquid tank 32 cannot limit the diameter of the tray body 1, and the diameter of the tray body 1 is not limited by the waste liquid tank 32 even if the edge of the tray body 1 is infinitely close to the liquid storage tank 31.

At the same time, the test tray also defines that the maximum diameter of the waste liquid outlet 311 of the reservoir 31 on the tray body 1 is smaller than the maximum diameter of the reservoir 31 on the tray body 1, as shown in fig. 4 and 5, when the first valve 34 is in the closed state, the test tray controls the liquid reagent 4 to leave the reservoir 31 from the waste liquid outlet 311 and enter the waste liquid tank 32 communicated with the reservoir 31 during the centrifugal movement. Therefore, the position of the waste liquid outlet 311 can determine the amount of liquid that the liquid reagent 4 can be stored in the reservoir 31 under the centrifugal action, and specifically, the amount of liquid stored in the reservoir 31 is actually the radial distance between the maximum diameter of the waste liquid outlet 311 on the tray body 1 and the maximum diameter of the reservoir 31 on the tray body 1, that is, in the radial direction from the outside to the inside of the tray body 1, the liquid reagent 4 enters the waste liquid tank 32 once reaching the waste liquid outlet 311 due to the centrifugal force, so that the larger the radial distance is, the more the amount of liquid stored in the reservoir 31 is, and the smaller the amount of liquid stored in the reservoir 31 is.

Therefore, by adjusting the relative positions of the reservoir 31 and the waste liquid tank 32, the excess liquid reagent 4 in the reservoir 31 enters the waste liquid tank 32 after the liquid reagent 4 in the reservoir 31 is quantified. However, since the maximum diameter of the waste liquid tank 32 and the waste liquid outlet 311 on the tray body 1 is smaller than the maximum diameter of the reservoir 31 on the tray body 1, and the maximum diameter of the reservoir 31 on the tray body 1 is smaller than the maximum diameter of the receiving groove 33 on the tray body 1, the liquid reagent 4 should normally flow in the direction from the waste liquid tank 32, the reservoir 31 to the receiving groove 33 under centrifugal motion.

Therefore, when the first valve 34 is in an open state, that is, when the liquid reagent 4 with a fixed amount needs to be controlled to enter the receiving groove 33 from the reservoir 31 in the testing step, not only the liquid reagent 4 with a fixed amount in the reservoir 31 will enter the receiving groove 33 under the centrifugal action, but also the waste liquid in the waste liquid groove 32 will flow back into the reservoir 31 and enter the receiving groove 33 under the centrifugal action, which may cause the previous quantifying operation to be meaningless.

In order to ensure that the excessive waste liquid in the liquid storage tank 31 cannot flow back to the liquid storage tank 31 from the waste liquid tank 32 and then enter the receiving groove 33 to affect biochemical reaction, a water absorbing material 35 is further arranged in the waste liquid tank 32, and after the liquid reagent 4 is forced to enter the waste liquid tank 32 under the centrifugal action of the test tray, the excessive waste liquid is absorbed by the water absorbing material 35, so that the waste liquid cannot flow back to the liquid storage tank 31 again even if the first valve 34 is in an open state.

It should be noted that the maximum diameter of the waste liquid tank 32 on the tray body 1 is smaller than the maximum diameter of the liquid storage tank 31 on the tray body 1, that is, the waste liquid tank 32 is located radially inward of the liquid storage tank 31 on the test tray, wherein the waste liquid tank 32 may have a partial overlap with the liquid storage tank 31 in the circumferential direction of the test tray, or the waste liquid tank 32 may be located completely radially inward of the liquid storage tank 31 as shown in fig. 2 and 3. Similarly, the maximum diameter of the reservoir 31 on the tray body 1 is smaller than the maximum diameter of the receiving groove 33 on the tray body 1, that is, the reservoir 31 is located radially inward of the receiving groove 33 on the test tray, wherein the reservoir 31 may partially overlap with the receiving groove 33 in the circumferential direction of the test tray, or the reservoir 31 may be located completely radially inward of the receiving groove 33, which is not limited herein.

In addition, the maximum diameter of the waste liquid outlet 311 on the tray body 1 is smaller than the maximum diameter of the liquid storage tank 31 on the tray body 1, that is, the waste liquid outlet 311 is located at a position radially inward of the liquid storage tank 31 on the test tray, and only the waste liquid outlet 311 is limited to be located at a position corresponding to the maximum diameter of the liquid storage tank 31, and a partial tank structure capable of quantifying the liquid reagent 4 can be defined in the liquid storage tank 31 through the waste liquid outlet 311. Therefore, those skilled in the art can specifically set the relative position relationship among the reservoir 31, the waste liquid tank 32 and the receiving tank 33, which are mutually matched, according to the requirement, so as to ensure that the liquid reagent 4 can be quantified and received.

As shown in fig. 6, the test tray may be formed by a tray body 1 and a cover body 2, and the cover body 2 covers the tray surface 11 of the tray body 1, so that the detection structure 3 on the tray surface 11 of the tray body 1 can be covered. Referring to fig. 11 and 12, when the test tray is used, the liquid reagent 4 may be first filled into the liquid storage tank 31, the liquid reagent 4 is filled, the tray body 1 and the cover body 2 are relatively assembled, and then the test tray is controlled to perform a centrifugal motion by the centrifugal device, so as to keep the first valve 34 in a closed state.

Referring to fig. 13 and 14, the liquid reagent 4 in the reservoir 31 moves outward in the radial direction of the test tray under the centrifugal force, and the liquid reagent 4 is deposited to a position radially outward in the reservoir 31 under the centrifugal force, which also corresponds to a state where the liquid reagent 4 falls (rests) by gravity when the test tray is vertical in the radial direction by gravity. The liquid reagent 4 is quantified when the part of the liquid reagent 4 that exceeds the waste liquid outlet 311 enters the waste liquid tank 32 and is absorbed and retained by the water absorbing material 35 therein.

Referring to fig. 15 and 16, when the quantification of the liquid reagent 4 in the reservoir 31 is completed, the first valve 34 may be opened to communicate between the reservoir 31 and the receiving groove 33, and the liquid reagent 4 may enter the receiving groove 33 by centrifugation. This makes it possible to prevent backflow and to quantify the amount of the liquid reagent 4, and to allow the liquid reagent 4 to smoothly enter the receiving groove 33 in a quantitative amount of liquid, thereby performing a biochemical reaction.

The water absorbing material 35 may be made of filter paper, cotton or nitrocellulose membrane, which has the ability to absorb and retain water rapidly. The absorbent material 35 may be secured within the associated waste reservoir 32 by gluing or nesting during installation. The tray body 1 and the cover body 2 may be made of any one of glass, polymethyl methacrylate (PMMA), Polystyrene (PS), Polycarbonate (PC), Cyclic Olefin Copolymer (COC), polyethylene terephthalate (PET), and the like. In addition, disk body 1 and lid 2 can be formed through the processing of shaping processing techniques such as moulding plastics, CNC, hot pressing, disk body 1 with lid 2 each other lid closes the equipment and can utilize any one or more bonding mode bonding in laser bonding, ultrasonic bonding, thermal compression bonding, adhesive bonding etc. to combine together to form a complete test tray.

The first valve 34 may be a phase change valve or other valve structure, wherein the phase change valve may be a wax, such as natural wax, paraffin wax, microcrystalline wax, or the like, or may be a thermoplastic resin or a phase change metal material, or the like. The phase change valve is characterized in that the phase change valve is provided with a groove structure for installing the phase change material, and the groove structure is provided with a plurality of grooves for installing the phase change material. Therefore, by utilizing the different melting points of the different phase-change materials, the different phase-change materials can be heated and melted separately or sequentially to open the fluid channel 334, or the different phase-change materials are cooled and solidified after being heated and expanded to block the fluid channel 334, so that the same microchannel can be operated to open and close for multiple times.

The phase change valve is preferably a wax valve because wax has a lower melting point temperature and better fluid properties. The wax material used for making the wax valve can be one or more pure waxes of vegetable wax, mineral wax, paraffin wax, microcrystalline wax and the like. Although these wax materials can be melted by heating using a heating element such as hot air, a resistance heater, a hot plate, etc., when it is necessary to shorten the response time for opening the wax valve, or when it is desired to use a light source having a specific wavelength as a heater, micro/nano particles may be doped in pure wax. The commonly used micro/nanoparticles may be one or more of carbon black particles, graphene powder, iron oxide particles, and the like. After the micro/nano particles are modified by amphiphilic modifying agents such as silanization coupling agents, fluorination coupling agents, surfactants and the like, hydrophobic groups can be exposed on the outer surfaces of the particles, so that the micro/nano particles are favorably dispersed in pure wax.

Referring to fig. 11 and 12, in one embodiment of the test tray, the test tray further comprises: a second valve 36, said second valve 36 being installed between said reservoir 31 and said waste liquid tank 32. When the second valve 36 is provided between the reservoir 31 and the waste liquid tank 32, the communication and closing state between the reservoir 31 and the waste liquid tank 32 can be controlled as required. Thus, when the reservoir 31 is filled with the liquid reagent 4, the first valve 34 and the second valve 36 are in the closed state so that the liquid reagent 4 is confined in the reservoir 31, thereby facilitating carrying of the test disc. The first valve 34 and the second valve 36 are opened separately when needed according to the testing procedure.

In one embodiment of the relative position of the reservoir 31 and the receiving groove 33, the test tray further comprises: a first passage 37, a maximum diameter of the reservoir 31 on the tray body 1 being smaller than a minimum diameter of the receiving groove 33 on the tray body 1, the reservoir 31 and the receiving groove 33 communicating through the first passage 37; the first valve 34 is mounted in the first passage 37. As shown in fig. 2 and 3, the reservoir 31 may be defined to be located completely radially inward of the receiving groove 33 on the test tray, and the first channel 37 between the reservoir 31 and the receiving groove 33 may be provided along the radial direction of the test tray, so that the liquid reagent 4 may flow more smoothly from the reservoir 31 to the receiving groove 33.

With continued reference to fig. 2 and 3, the test tray further comprises: a second passage 38, through which the waste liquid tank 32 and the reservoir 31 communicate. The second channel 38 communicates with a waste liquid inlet 321 of the waste liquid tank 32, and the maximum diameter of the waste liquid inlet 321 on the tray body 1 is equal to the maximum diameter of the waste liquid tank 32 on the tray body 1; and/or the second channel 38 is communicated with the waste liquid outlet 311, and the waste liquid outlet 311 is positioned between the maximum diameter and the minimum diameter of the liquid storage tank 31 on the tray body 1.

Therefore, the excess waste liquid in the reservoir 31 can be discharged to the waste liquid tank 32 through the second channel 38. The maximum diameter of the waste liquid inlet 321 on the tray body 1 is equal to the maximum diameter of the waste liquid tank 32 on the tray body 1, that is, the waste liquid inlet 321 is arranged at the radial outermost end of the waste liquid tank 32 on the test tray, so that the waste liquid tank 32 can be ensured to be positioned at a radial inward position, the influence on the diameter size of the tray body 1 can be reduced to the maximum extent, and meanwhile, the waste liquid can be ensured to enter the waste liquid tank 32 more smoothly.

Furthermore, the waste outlet 311 is located between the maximum diameter and the minimum diameter of the reservoir 31 on the tray 1, and the quantifiable amount of the liquid reagent 4 in the reservoir 31 can be defined by adjusting the radial position of the waste outlet 311 on the reservoir 31, the greater the value of the quantifiable amount the more radially inward the waste outlet 311 (the more radially inward the maximum diameter of the waste outlet 311). Conversely, the smaller the quantifiable value. Therefore, those skilled in the art can adjust the quantifiable value according to the requirement, and the limitation and the description are not given here.

Further, the test tray further includes: a quality control reagent provided on the water absorbing material 35.

The quality control reagent has a function of reacting with the liquid reagent 4 and developing a color, and can change the color of a part or the whole area of the water absorbent material 35 after the quality control reagent reacts with the liquid reagent 4. Therefore, once the color of the absorbent material 35 changes, it can be verified that the excess waste liquid of the liquid reagent 4 in the reservoir 31 enters the waste liquid tank 32 and is adsorbed in the absorbent material 35, which means that the liquid reagent 4 in the reservoir 31 has reached the maximum liquid amount that can be quantified, i.e. the quantification operation of the liquid reagent 4 in the reservoir 31 is completed, thereby achieving the purpose of controlling the quality of the liquid reagent 4.

The quality control reagent may be selected from an acid-base indicator, such as phenolphthalein indicator, bromophenol blue indicator, neutral red indicator, methyl red indicator, etc., which may exhibit different colors depending on the pH of the liquid reagent 4 in the reservoir 31. For example, when the phenolphthalein indicator is used as the quality control agent and the water absorbent material 35 is white, the water absorbent material 35 may still have the original color after the phenolphthalein indicator is sprayed and dried on the water absorbent material 35. When the liquid reagent 4 with pH 9.5 enters the waste liquid tank 32 and reacts with the phenolphthalein indicator, the water absorbent material 35 turns pink accordingly. Therefore, the excess waste liquid of the liquid reagent 4 in the liquid storage tank 31 can be judged to enter the waste liquid tank 32 through the color change of the water absorbing material 35 from white to pink, that is, the amount of the liquid reagent 4 in the liquid storage tank 31 is enough, because if the amount of the liquid reagent 4 is not enough, no excess waste liquid can enter the waste liquid tank 32.

In addition, those skilled in the art can select a quality control reagent that can be used in a complex reaction and that can develop a color, depending on the type of the specific liquid reagent 4. And to the chromogenic reaction of water absorbing material 35, can discern through the image recognition device that cooperates, the image recognition device can cooperate the assembly to be used for carrying out centrifugal operation to the test disc on the centrifugal device, certainly also can set up independently, and the technical staff in the art can select different structural style according to the user demand, does not need to be repeated here. In addition, the quality control reagent may be embedded in the water absorbing material 35 or sprayed on the water absorbing material 35 according to the kind, and if necessary, the drying operation may be performed on the water absorbing material, which is not limited herein.

Referring to fig. 10 or fig. 20, illustrating the structure or position of the through hole 21, at least one through hole 21 corresponding to the detection structure 3 is formed on the cover 2. The number and the position of the through holes 21 are not limited, and a plurality of through holes may be provided and respectively correspond to any position of the first valve 34, the second valve 36, the liquid storage tank 31, and the like, so that after the cover body 2 and the tray body 1 are mutually covered and assembled, corresponding operations can be performed on different positions of the detection structure 3.

For example, after any one of the through holes 21 is communicated with the reservoir 31, it can be used as a sample adding hole for injecting the liquid reagent 4, and at this time, when the test tray is used, the tray body 1 and the cover body 2 are assembled relatively, and then the liquid reagent 4 is injected into the reservoir 31 through the through hole 21. In addition, other through holes 21 may be provided as air holes.

In one embodiment of the detection structure 3, as shown in fig. 7 to 9, the waste liquid tank 32 comprises a first waste liquid tank 322, the reservoir 31 comprises a first reservoir 312 and a second reservoir 313, and the receiving tank 33 comprises a first receiving tank 331 and a second receiving tank 332; the first waste liquid tank 322 is simultaneously communicated with the first reservoir 312 and the second reservoir 313, the first reservoir 312 is communicated with the first receiving groove 331, and the second reservoir 313 is communicated with the second receiving groove 332.

The detection structure 3 employs one waste liquid tank 32 to collect waste liquid from two reservoirs 31, that is, the first waste liquid tank 322 is simultaneously communicated with the first reservoir 312 and the second reservoir 313, and at the same time, each reservoir 31 is cooperatively communicated with one receiving groove 33, that is, the first reservoir 312 is communicated with the first receiving groove 331, and the second reservoir 313 is communicated with the second receiving groove 332.

Therefore, the test structure formed by the structure can carry out quantitative and quality control operation on two different liquid reagents 4 through a single test structure, and during specific operation, redundant waste liquid of the liquid reagents 4 in the first liquid storage tank 312 or the second liquid storage tank 313 can be collected by the waste liquid tank 32 according to the types of the different liquid reagents 4 and related test requirements, and color reaction is carried out by matching with the quality control reagent.

Based on the above structure, in a specific embodiment, the white water absorbing material 35 sprayed with the phenolphthalein indicator and dried may be integrated in the first waste liquid tank 322 of the test tray, and different kinds of liquid reagents 4, such as the first liquid reagent 4 and the second liquid reagent 4, may be loaded in the first reservoir 312 and the second reservoir 313, respectively. The first liquid reagent 4 is defined to have a pH of 9.5 and the second liquid reagent 4 has a pH of 7.0. When the second valve 36 between the first reservoir 312 and the first waste liquid tank 322 is in the open state and the other valves are in the closed state, the excess waste liquid of the first liquid reagent 4 flows into the waste liquid tank 32 under the centrifugal action and is absorbed by the water absorbent material 35, thereby completing the quantification of the first liquid reagent 4.

At this time, the phenolphthalein indicator in the water absorbent material 35 is redissolved and turns the water absorbent material 35 into pink. The image recognition device may determine that the amount of the first liquid reagent 4 added to the first reservoir 312 is sufficient according to the phenomenon that the water absorbent material 35 changes from white to pink, and thus, the subsequent operations may be performed continuously. Once the image recognition device cannot detect the color change of the water absorbing material 35, it indicates that the liquid amount of the first liquid reagent 4 is insufficient, and therefore the quality control link cannot be passed, and the whole test operation needs to be stopped.

After the quantification of the first liquid reagent 4 is completed, the second valve 36 between the second reservoir 313 and the first waste liquid tank 322 may be continuously opened, so that the excess second liquid reagent 4 enters the first waste liquid tank 322 by the same method as described above, thereby achieving the quantification of the second liquid reagent 4. Since the second liquid reagent 4 has a pH of 7.0, when it is absorbed by the water absorbing material 35, it neutralizes the first liquid reagent 4 that was originally absorbed by the water absorbing material 35, so that the pink water absorbing material 35 turns white again.

At this time, the image recognition device can determine that the amount of the second liquid reagent 4 added to the second reservoir 313 is sufficient according to the phenomenon that the absorbent material 35 changes from pink to white, and further, the subsequent operation can be continuously performed. Similarly, once the image recognition device cannot detect the color change, it indicates that the liquid amount of the second liquid reagent 4 is insufficient, and therefore the whole test operation needs to be stopped due to the failure of the quality control link.

When the other first valves 34 are opened after the first liquid reagent 4 and the second liquid reagent 4 are both dosed, the dosed first liquid reagent 4 and the dosed second liquid reagent 4 are released into the first receiving groove 331 and the second receiving groove 332, respectively.

In another embodiment of the detection structure 3, as shown in fig. 17 to 19, the waste liquid tank 32 further comprises a second waste liquid tank 323, the reservoir 31 further comprises a third reservoir 314, and the receiving tank 33 further comprises a third receiving tank 333; the second waste liquid tank 323 communicates with the third reservoir 314, and the third reservoir 314 communicates with the third receiving groove 333.

The detection structure 3 may further include a waste liquid tank 32, and the waste liquid tank 32 is used to simultaneously and separately communicate the liquid storage tank 31 and the receiving tank 33, that is, the second waste liquid tank 323 communicates with the third liquid storage tank 314, and the third liquid storage tank 314 communicates with the third receiving tank 333. Therefore, the detection structure 3 can perform quantitative and quality control operations on three different liquid reagents 4 through a single test structure, and during specific operations, according to the types of the different liquid reagents 4 and related test requirements, the redundant waste liquid of the liquid reagent 4 in the first reservoir 312 or the second reservoir 313 can be collected by the first waste liquid tank 322, and the redundant waste liquid of the liquid reagent 4 in the third reservoir 314 can be collected by the second waste liquid tank 323, and color reaction can be performed by matching with the quality control reagent.

It should be noted that, the number and the matching and communicating structure of the waste liquid tank 32, the liquid storage tank 31 and the receiving tank 33 in the detecting structure 3 are not limited to the above list, and those skilled in the art can flexibly adjust the number and the matching and communicating structure of the required waste liquid tank 32, liquid storage tank 31 and receiving tank 33 according to the needs, so as to meet the testing requirements of various liquid reagents 4 and other different testing requirements, which is not described herein again.

With continued reference to fig. 17-19, the test tray further comprises: a fluid passage 334 through which the first receiving groove 331, the second receiving groove 332, and the third receiving groove 333 communicate in sequence. Therefore, the liquid reagent 4 received between the first receiving groove 331, the second receiving groove 332, and the third receiving groove 333 can communicate among the three through the fluid passage 334. Therefore, the single detection structure 3 not only can individually test different liquid reagents 4, but also can perform a combined operation of a complete chemical experiment, such as an enzyme-linked immunosorbent assay, through the detection structure 3.

With continued reference to fig. 17-19, the test tray further comprises: sample introduction slot 335, sample processing channel 336, and siphon channel 337; the sample processing channel 336 is in communication with the sample introduction slot 335, one end of the siphon channel 337 is in communication with the sample introduction slot 335, and the other end of the siphon channel 337 is in communication with the first receiving slot 331. Thus, in one embodiment of the detection structure 3, a sample reagent that is intended to react with the liquid reagent 4 can be processed through the sample introduction groove 335, the sample processing channel 336 and the siphon channel 337 into each receiving groove 33 to react with the liquid reagent 4.

Based on the above structure, in a specific embodiment, the structure of the test tray is clearly illustrated by taking an enzyme-linked immunosorbent assay as an example. In order to improve the convenience of use of the test tray and reduce the test error, a reaction buffer (pH 7.0), a washing buffer (pH 7.5) and a substrate solution (pH 9.5) required for the enzyme-linked immunochemical luminescence reaction are pre-embedded in the test tray. The reaction buffer (pH 7.0), the washing buffer (pH 7.5), and the substrate solution (pH 9.5) are pre-embedded in the first reservoir 312, the third reservoir 314, and the second reservoir 313, respectively. Since the channels communicating with the first reservoir 312, the third reservoir 314, and the second reservoir 313 are sealed by the first valve 34, preferably a normally closed wax valve, the reaction buffer, the washing buffer, and the substrate solution are always kept in the first reservoir 312, the third reservoir 314, and the second reservoir 313 until the first valve 34 is opened. In addition, an enzyme-labeled antibody, a biotinylated antibody, and streptavidin-modified magnetic beads are pre-embedded in the first receiving groove 331 in a dry form.

As shown in fig. 17 and 18. In conducting the experiment, a whole blood (or serum, plasma) sample is first added to the sample processing channel 336 from the sample introduction slot 335 by a pipette, and then the test tray is assembled to a centrifuge device, which allows the blood cells and serum in the whole blood sample to be separated into layers within the sample processing channel 336. Wherein the upper layer is serum and the lower layer is blood cells. The interface between the serum layer and the blood cell layer is flush with the inlet of the siphon channel connected to the sample processing channel 336, and since the siphon channel is modified by the hydrophilic modification reagent, the serum will flow into the siphon channel when the whole blood sample is layered and the centrifugal force is less than the capillary force of the siphon channel. The centrifugal force is then increased to transfer the serum into the first receiving well 331 while the blood cells remain in the sample processing channel 336.

Meanwhile, the reaction buffer and the substrate solution may be quantified and controlled according to the quantification and control method described above, and the quantified reaction buffer may be released into the first receiving groove 331. Specifically, the white water absorbing material 35 integrated in the first waste liquid tank 322 is sprayed with the phenolphthalein indicator in advance and is dried. When the second valve 36 between the second reservoir 313 and the first waste liquid tank 322 is in an open state and the other valves are in a closed state, the excess substrate liquid in the second reservoir 313 is discharged into the first waste liquid tank 322 by centrifugation, and the absorbent material 35 turns pink after the substrate liquid is absorbed by the absorbent material 35 therein. This indicates that the amount of the substrate liquid is sufficient for the subsequent operations to be continued.

By continuing to open the second valve 36 between the first reservoir 312 and the first waste liquid tank 322, the excess reaction buffer in the first reservoir 312 will be discharged into the first waste liquid tank 322 by centrifugation, and the reaction buffer will be absorbed by the absorbent material 35 therein to restore the absorbent material 35 from pink to white. This indicates that the reaction buffer is in sufficient volume to continue the subsequent operations. The reaction buffer may then be released into the first receiving groove 331 after the quantitative amount is determined. At the moment, the pre-embedded enzyme-labeled antibody, biotinylated antibody and streptavidin-modified magnetic microbead can be redissolved by the reaction buffer solution and serum, and can rapidly participate in the reaction under the condition of centrifugal oscillation.

Subsequently, the excess washing buffer may be drained from the third reservoir 314 into the second waste liquid tank 323 under centrifugation. A neutral red indicator may be disposed in the second waste liquid tank 323, which may first turn the originally white water absorbing material 3515 into red. When the water-absorbing material 35 in the second waste liquid tank 323 absorbs the washing buffer, the washing buffer reacts with the neutral red indicator to make the water-absorbing material 35 turn yellow-orange. This indicates that the amount of washing buffer is sufficient for the subsequent operations to be carried out.

When the quantified washing buffer is released into the third receiving groove 333 by centrifugation, the magnetic beads in the first receiving groove 331 can be driven to be transferred into the third receiving groove 333 by a matched magnetic bead transfer device. The magnetic beads can wash away the impurity molecules that are not strongly bound there. Subsequently, the quantified substrate solution is discharged to the second receiving well 332 by centrifugation. At this time, the magnetic beads may be continuously transferred from the third receiving groove 333 into the second receiving groove 332 by the magnetic bead transfer means. A chemiluminescent reaction may occur within the second receiving slot 332 which in turn generates an optical signal that is captured and recorded by a suitable optical detection device, thereby completing the entire testing process. In the present invention, the centrifugal device, the image recognition device, the magnetic bead transfer device, the optical detection device, and the like may be selected by a person skilled in the art according to the needs, and the selection is not limited herein.

With continued reference to fig. 17 and 18, the detection structures 3 are multiple groups, and the multiple groups of detection structures 3 are arranged in a circumferential array with the central axis of the tray body 1 as the axis. Therefore, because a plurality of groups of detection structures 3 are arranged on a single test disc, the test operation of a plurality of liquid reagents 4 can be synchronously performed on the single test disc, and the test efficiency is greatly improved.

an image recognition device having a recognition portion provided corresponding to the waste liquid tank 32 of the test tray and/or a magnetic bead transfer device having a control portion provided corresponding to the receiving groove 33.

When the test system adopts the test disc for reaction, the test disc can be arranged on the driving part of the centrifugal device through the shaft hole 12, so that the centrifugal motion of the test disc is realized through the driving of the centrifugal device. The image recognition means may recognize the water absorbing material 35 to which the quality control agent is added to determine whether or not it is discolored. The magnetic microbead transfer device can make the test disc have magnetic particle control function at the same time, so as to carry out relevant biochemical, immune and molecular diagnosis operation, such as enzyme-linked immunosorbent assay.

In addition, when the phase change valve is the first valve 34, the second valve 36 and the like, the test system further comprises a temperature control device matched with the phase change valve to realize the opening operation of the phase change valve by controlling the temperature of the phase change valve. The detailed structure, functional principle and technical effect of the test disc have been described in detail in the foregoing. Therefore, any technical content related to the test disc can be referred to the above description, and will not be described herein again.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A test tray, comprising:

the tray body is provided with at least one group of detection structures;

the waste liquid tank is communicated with a waste liquid outlet of the liquid storage tank, and the maximum diameters of the waste liquid tank and the waste liquid outlet on the tray body are smaller than the maximum diameter of the liquid storage tank on the tray body; the waste liquid outlet is communicated with a waste liquid inlet of the waste liquid tank, and the maximum diameter of the waste liquid inlet on the tray body is equal to the maximum diameter of the waste liquid tank on the tray body;

a water absorbent material disposed within the waste tank.

2. The test tray of claim 1, further comprising:

a second valve mounted between the reservoir and the waste reservoir.

3. The test tray of claim 1, further comprising:

the first valve is mounted within the first passage.

4. The test tray of claim 1, further comprising:

the second channel is communicated with a waste liquid inlet of the waste liquid groove; and/or the second channel is communicated with the waste liquid outlet, and the waste liquid outlet is positioned between the maximum diameter and the minimum diameter of the liquid storage tank on the tray body.

5. The test tray of claim 1, further comprising:

and the quality control reagent is arranged on the water absorbing material.

6. The test tray of claim 1, further comprising:

the cover body is covered with the disc surface of the disc body relatively.

7. The test tray of claim 6, wherein the cover has at least one through hole corresponding to the detecting structure.

8. The test tray of any one of claims 1 to 7, wherein the waste liquid tank comprises a first waste liquid tank, the reservoir comprises a first reservoir and a second reservoir, and the receiving groove comprises a first receiving groove and a second receiving groove; the first waste liquid tank is communicated with the first liquid storage tank and the second liquid storage tank at the same time, the first liquid storage tank is communicated with the first receiving groove, and the second liquid storage tank is communicated with the second receiving groove.

9. The test tray of claim 8, wherein the waste liquid tank further comprises a second waste liquid tank, the reservoir further comprises a third reservoir, and the receiving groove further comprises a third receiving groove; the second waste liquid tank is communicated with the third liquid storage tank, and the third liquid storage tank is communicated with the third receiving groove.

10. The test tray of claim 9, further comprising:

11. The test tray of claim 10, further comprising:

12. The test tray according to any one of claims 1 to 7, wherein the plurality of sets of the detection structures are arranged in a circumferential array around the central axis of the tray body.

13. The test disc according to any of claims 2-7, wherein the first valve and/or the second valve is a phase change valve.

14. A test system comprising a test disc according to any one of claims 1-13; further comprising: