CN111484125A - Method and system for detecting content of active microorganisms in biological membrane of MBBR (moving bed biofilm reactor) system - Google Patents

Method and system for detecting content of active microorganisms in biological membrane of MBBR (moving bed biofilm reactor) system Download PDF

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CN111484125A
CN111484125A CN202010318726.5A CN202010318726A CN111484125A CN 111484125 A CN111484125 A CN 111484125A CN 202010318726 A CN202010318726 A CN 202010318726A CN 111484125 A CN111484125 A CN 111484125A
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active
mbbr
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王晓东
陈珊珊
毕学军
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Qingdao University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1236—Particular type of activated sludge installations
    • C02F3/1268—Membrane bioreactor systems
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/02—Temperature
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/22—O2
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

The invention provides a method and a system for detecting the content of active microorganisms in a biological membrane of an MBBR (moving bed biofilm reactor) system, belonging to the technical field of sewage treatment and environmental microorganism detection and analysis. The method comprises the following steps: measuring the oxygen consumption respiration rate of the biological membrane of the MBBR system; and calculating the content of active microorganisms on the biological membrane by using an active sludge model. The method can realize the quantification of the content of the active microorganisms on the biological membrane by monitoring the oxygen consumption and respiration rate of the biological membrane without complex biological membrane pretreatment and combining an active sludge model, and has the advantages of simplicity, convenience, high accuracy and good practical application value.

Description

Method and system for detecting content of active microorganisms in biological membrane of MBBR (moving bed biofilm reactor) system
Technical Field
The invention belongs to the technical field of sewage treatment and environmental microorganism detection and analysis, and particularly relates to a method and a system for detecting the content of active microorganisms in a biological membrane of an MBBR (moving bed biofilm reactor) system.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
Moving Bed Biofilm Reactor (MBBR) technology was in the wake in norway in the nineties of the last century, and by virtue of its compact structure, higher active biomass and stable removal efficiency, it is widely used in new sewage treatment plants and in upgrading projects of sewage treatment plants. Microorganisms in the MBBR system are attached and grown on the filler in the form of a biological film, and the content of active microorganisms on the biological film is an important factor influencing the treatment effect of the system. The microorganisms in the biomembrane can be divided into autotrophic microorganisms and heterotrophic microorganisms, the activity and the quantity of the autotrophic microorganisms directly influence the treatment effect of ammonia nitrogen, and the active biomass of the heterotrophic microorganisms is closely related to the removal of COD and the denitrification process. Because each independent reaction zone of the same MBBR system intercepts the suspended filler, different microorganism distributions are formed in each reaction zone under the action of environment selection. Therefore, by studying the contents of active heterotrophic microorganisms and active autotrophic organisms in the MBBR system and the distribution of the active heterotrophic microorganisms and the active autotrophic organisms in each chamber, the treatment efficiency, the treatment capacity and the operation stability of the system can be effectively evaluated.
At present, the suspended solid concentration (M L SS) and the volatile suspended solid concentration (M L VSS) are commonly used as the evaluation indexes of the content of active microorganisms in a sewage treatment plant, but the two indexes comprise partial residues of the spontaneous oxidation of the microorganisms, inert organic matters and adsorbed inorganic matters besides the active microorganism substances, so that the inventor finds that the evaluation of the content of the active microorganisms has inaccuracy and instability.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the method and the system for quantitatively detecting the content of the active microorganisms, which have the advantages of simple method, accurate measurement and no need of complex biomembrane pretreatment, can realize the determination of the ratio of the active autotrophic microorganisms to the heterotrophic microorganisms in each chamber in the MBBR system, and has good value of practical application.
The invention is realized by the following technical scheme:
in a first aspect of the present invention, there is provided a method for detecting the content of active microorganisms in a biofilm of an MBBR system, the method comprising:
measuring the oxygen consumption respiration rate of the filler biofilm in the MBBR system;
and calculating the content of active microorganisms on the biological membrane by using an active sludge model.
The specific method for measuring the oxygen consumption respiration rate of the biological membrane of the MBBR system comprises the following steps:
placing the filler in the MBBR system in a biological reaction system, and recording the dissolved oxygen concentration in the system along with time change in real time;
and drawing a dissolved oxygen-time curve, and calculating oxygen consumption respiration rate OURs.
Wherein, the biological reaction system comprises a biological reaction device, a stirring device, an aeration device, a dissolved oxygen measuring device and a temperature measuring device.
Wherein, the biological reaction device is a sealable transparent device, such as a cylindrical reactor made of organic glass; specifically, the filler in the MBBR system is placed in a biological reaction device;
the stirring device is arranged in the biological reaction device, and can be stirred in a mechanical stirring mode of a stirring paddle so as to ensure that the filler is always in a good fluidization state;
the aeration device can be an aeration head connected with an aeration pump, and the aeration head extends into the bottom of the biological reaction device.
The dissolved oxygen measuring device can be a portable dissolved oxygen meter, and the measuring frequency can be 1-2 min (preferably 1min) so as to record the dissolved oxygen concentration in the reaction process in real time.
The temperature measuring device can be a glass ball thermometer, so that the temperature during the reaction process can be measured.
The activated sludge model is ASM 1.
The method for calculating the content of the active microorganisms on the biomembrane by using the activated sludge model specifically comprises the step of calculating the content and/or the proportion of the active microorganisms of the autotrophic microorganisms and the heterotrophic microorganisms on the basis of the oxygen consumption respiration rate OURs and the activated sludge model ASM 1.
In a second aspect of the present invention, there is provided a system for detecting the content of active microorganisms in a biofilm of an MBBR system, wherein the system is the above biological reaction system.
In a third aspect of the invention, there is provided the use of the above method and/or system for detecting the content of active microorganisms in a biofilm of an MBBR system in the determination of the content and/or ratio of active autotrophic and heterotrophic microorganisms in each chamber of the MBBR system.
The beneficial technical effects of one or more technical schemes are as follows:
the technical scheme provides the quantitative detection method for the content of the active microorganisms, which is simple, accurate in measurement and free of complex biofilm pretreatment, can realize the determination of the proportion of the active autotrophic microorganisms and the heterotrophic microorganisms of each chamber in the MBBR system, and effectively solves the problems of high difficulty in biofilm pretreatment, inaccurate determination, high equipment and technical requirements and the like of the conventional detection method for the determination of the content of the active microorganisms on the biofilm on the filler in the MBBR system.
According to the technical scheme, the oxygen consumption and respiration rate of the biological membrane is measured by monitoring the oxygen consumption and respiration rate of the biological membrane, complex biological membrane pretreatment is not needed, quantification of the content of active microorganisms on the biological membrane can be realized by combining an active sludge model, and the method is simple, convenient and high in accuracy, so that the method has a good value in practical application.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a schematic view of an apparatus for measuring the content of active microorganisms on a biofilm of an MBBR system in example 1 of the present invention;
FIG. 2 is a schematic diagram illustrating calculation of oxygen consumption respiration rate of the biofilm in example 1 of the present invention;
the reference numbers in the figures represent: 101-biological reaction device, 102-stirring device, 103-aeration device, 104-dissolved oxygen measuring device, 105-temperature measuring device, 201-first stage linear interval, 201-second stage linear interval, 203-third stage linear interval.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise. It is to be understood that the scope of the invention is not to be limited to the specific embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.
As mentioned above, the measurement of the content of the active microorganisms in the biological membrane in the current MBBR system generally has the problems of inaccurate measurement, high equipment and technical requirements, difficult pretreatment of the biological membrane and the like.
In view of the above, in one embodiment of the present invention, there is provided a method for determining the content of active microorganisms on a biofilm of suspended fillers in an MBBR system, the method comprising:
measuring the oxygen consumption respiration rate of the filler biofilm in the MBBR system;
and calculating the content of active microorganisms on the biological membrane by using an active sludge model.
In another embodiment of the present invention, the specific method for determining the oxygen consumption respiration rate of the MBBR system biofilm comprises:
placing the filler in the MBBR system in a biological reaction system, and recording the dissolved oxygen concentration in the system along with time change in real time;
and drawing a dissolved oxygen-time curve, and calculating oxygen consumption respiration rate OURs.
In a further embodiment of the present invention, before placing the packing in the biological reaction system, the packing is preferably pretreated, and the pretreatment method specifically comprises rinsing the packing 3 to 5 times with clean water; the pretreatment method is very simple and convenient, and meanwhile, the error caused by the detection by peeling the biological membrane by the conventional technology is avoided.
In yet another embodiment of the present invention, during the oxygen consumption respiration rate of the MBBR system biofilm, the oxygen consumption activity of the active microorganisms is divided into three phases;
the first stage is that aeration is started, and when the concentration of dissolved oxygen rises to 9 mg-L-1Stopping aeration, simultaneously starting the stirring device, not adding medicament, recording the temperature and recording the dissolved oxygen concentration in real time until the concentration is reduced to 0.2 mg-L-1Stopping recording, wherein the stage is the endogenous respiration stage of the active microorganisms;
the second stage is that aeration is started, and when the concentration of dissolved oxygen rises to 9 mg-L-1Stopping aeration, starting the stirring device, and adding 20mg of N. L-1The concentration of the dissolved oxygen is recorded in real time until the concentration is reduced to 0.2 mg-L-1Stopping recording, and mainly performing metabolic activity of the active autotrophic microorganisms and endogenous respiratory activity of the active heterotrophic microorganisms in the stage;
in the third stage, aeration is started, and when the concentration of dissolved oxygen rises to 9 mg-L-1Stopping aeration, starting the stirring device, and adding 20mg of N. L-1Ammonium chloride solution (D), 250mg COD L-1And 20mg of N. L-1The concentration of dissolved oxygen is recorded in real time until the concentration is reduced to 0.2 mg-L-1Stopping recording, and inhibiting the metabolism of autotrophic microorganisms due to addition of sufficient nitrification inhibitorThe stage is completely the metabolic activity of the active heterotrophic microorganism;
drawing a dissolved oxygen-time curve of the three phases, and further obtaining an endogenous respiration rate OURenOxygen consumption rate OUR of autotrophic bacteriaAAnd heterotrophic bacteria oxygen consumption Rate OURH。
In still another embodiment of the present invention, the biological reaction system includes a biological reaction apparatus, a stirring apparatus, an aeration apparatus, a dissolved oxygen measuring apparatus, and a temperature measuring apparatus.
In another embodiment of the present invention, the bioreactor is a sealable transparent device, such as a cylindrical reactor made of organic glass; specifically, the filler in the MBBR system is placed in a biological reaction device;
in another embodiment of the present invention, the stirring device is disposed in the biological reaction device, and the stirring device can perform stirring by a mechanical stirring manner of a stirring paddle to ensure that the filler is always in a good fluidized state;
in another embodiment of the present invention, the aeration device may be an aeration head connected to an aeration pump, and the aeration head extends into the bottom of the biological reaction device.
In another embodiment of the present invention, the dissolved oxygen measuring device may be a portable dissolved oxygen meter (hash HQ40d), and the measuring frequency may be 1 to 2min (preferably 1min) to record the dissolved oxygen concentration during the reaction in real time.
In another embodiment of the present invention, the temperature measuring device may be a glass bulb thermometer, so as to measure the temperature during the reaction.
In yet another embodiment of the present invention, the activated sludge model is ASM 1.
In another embodiment of the present invention, the calculating the content of the active microorganisms on the biofilm by using the activated sludge model is specifically calculating the content and/or the ratio of the active microorganisms of the autotrophic microorganisms and the heterotrophic microorganisms based on the oxygen consumption respiration rate OURs and the activated sludge model ASM 1.
In another embodiment of the present invention, a system for detecting the content of active microorganisms in a biofilm of an MBBR system is provided, wherein the system is the above biological reaction system.
In another embodiment of the present invention, there is provided a use of the above method and/or system for detecting the content of active microorganisms in a biofilm of an MBBR system in the determination of the content and/or ratio of active autotrophic and heterotrophic microorganisms in each chamber of the MBBR system.
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
Example 1
The embodiment realizes the determination of the aerobic respiration rate of microorganisms on a biological film through a biological reaction system, which is shown in figure 1 and comprises a biological reaction device 101, a stirring device 102, an aeration device 103, a dissolved oxygen determination device 104 and a temperature determination device 105, wherein the biological reaction device is the main body of the whole system and is a closed transparent organic glass cylindrical reactor, the biological film in a system with the volume of 5L is subjected to reaction, the stirring device is arranged in the biological reaction device to ensure that a filler is always in a good fluidized state, the aeration device is a small aeration head connected with an aeration pump, the aeration head extends into the bottom of the biological reaction device, the dissolved oxygen determination device is a portable dissolved oxygen meter (Hash HQ40d), the determination frequency is 1min, the dissolved oxygen concentration in the reaction process is recorded in real time, and the temperature determination device is a glass ball thermometer, the temperature determination in the reaction process is carried out, and the closed state is always kept in the system determination process.
The method for measuring the content of the active microorganisms on the biological membrane of the MBBR system comprises the following steps:
(1) quantitatively taking out the fillers from each chamber of the MBBR pilot-scale system, recording the number of the fillers, flushing the fillers for 3 to 5 times by using tap water, placing the fillers in a biological reaction device, adding the tap water to 5L, and starting to measure the oxygen consumption respiration rate of the active microorganisms.
(2) Consumption of active microorganismsThe oxygen activity is divided into three stages, wherein the dosing method of each stage is shown in Table 1. the first stage, aeration is started, and when the dissolved oxygen concentration rises to 9 mg-L-1Stopping aeration, simultaneously starting the stirring device, not adding medicament, recording the temperature and recording the dissolved oxygen concentration in real time until the concentration is reduced to 0.2 mg-L-1Stopping recording, the period is the endogenous respiration of active microorganism, the second period is that aeration is started, when the concentration of dissolved oxygen rises to 9 mg. L-1Stopping aeration, starting the stirring device, and adding 20mg of N. L-1The concentration of the dissolved oxygen is recorded in real time until the concentration is reduced to 0.2 mg-L-1Stopping recording, performing metabolism of active autotrophic microorganisms and endogenous respiration of active heterotrophic microorganisms, and aerating until the dissolved oxygen concentration is 9 mg-L-1Stopping aeration, starting the stirring device, and adding 20mg of N. L-1Ammonium chloride solution (D), 250mg COD L-1And 20mg of N. L-1The concentration of dissolved oxygen is recorded in real time until the concentration is reduced to 0.2 mg-L-1The recording is stopped, and the metabolic activity of the autotrophic microorganisms is inhibited by adding enough nitrification inhibitor, so that the metabolic activity of the active heterotrophic microorganisms is completely obtained in the stage.
(3) The dissolved oxygen-time curves for the three phases are plotted as shown in figure 2. Selecting interval parts (201, 202, 203) in which the concentration of the dissolved oxygen is in linear relation with the time, calculating the steady state oxygen consumption respiration rate OURs, wherein the absolute value of the slope is the corresponding OURs (OURs |, the unit is mg O |)2·L-1·min-1) The oxygen consumption respiration rates of the three phases are sequentially recorded as OUR1、OUR2、OUR3. Further calculating the endogenous respiration rate OURenOxygen consumption rate OUR of autotrophic bacteriaAOxygen consumption rate OUR of heterotrophic bacteriaHWherein, in the step (A),
endogenous respiration rate, OURen＝OUR1；
Oxygen consumption Rate, OUR, of autotrophic bacteriaA＝OUR2-OUR1；
Heterotrophic bacteria oxygen consumption Rate, OURH＝OUR3。
(4) Calculating the active heterotrophic biomass and the active autotrophic biomass according to the formulas (the following formulas (1) and (2)) in an activated sludge model ASM1, further simplifying the formulas (1) and (2) to obtain a formula (3) and a formula (4) because the amount of COD and ammonia nitrogen is sufficient in the test process, and finally calculating the active heterotrophic microorganism X according to the formulas (3) and (4)HAnd autotrophic microorganism XAThe contents of (A) and the definitions of the components in the formulae (1) to (4) are shown in Table 2.
Figure BDA0002460524990000091
Figure BDA0002460524990000092
Figure BDA0002460524990000093
Figure BDA0002460524990000094
If the ambient temperature of the test is not the standard temperature of ASM1 (20 ℃), then the maximum rate of increase in specific area (. mu.M) is requiredH,Max、μA,Max) Temperature correction is carried out, and the temperature coefficient adopts the suggested value (a) of ASM2H＝1.07，aA1.12), the default values of ASM1 are used for the remaining parameters as shown in equations (5) and (6), and the specific values are shown in table 3.
μH,Max＝μH,Max,20·a(T-20)Formula (5)
μA,Max＝μA,Max,20·a(T-20)Formula (6)
Calculating to obtain the active heterotrophic microorganism XHAnd autotrophic microorganism XAThe ratio of the active heterotrophic microorganism to the active autotrophic microorganism can be obtained from the formulas (7) and (8), and the specific calculation results are shown in Table 4.
Figure BDA0002460524990000101
Figure BDA0002460524990000102
TABLE 1 breath test method for determining active biomass in biofilms
Figure BDA0002460524990000103
TABLE 2 variables and model parameters for the growth model of active heterotrophic biomass and autotrophic biomass
Figure BDA0002460524990000104
Figure BDA0002460524990000111
TABLE 3 ASM1 Default values for parameters
Figure BDA0002460524990000112
TABLE 4 concrete calculation results of active microorganism content
Item Numerical value Unit of
OUR1 0.25385 mg O2·L-1·min-1
OUR2 0.27422 mg O2·L-1·min-1
OUR3 0.979 mg O2·L-1·min-1
OURA 0.02037 mg O2·L-1·min-1
OURH 0.979 mg O2·L-1·min-1
Temperature of 28 ℃
μA,,0 1.98 day-1
μH,,0 10.31 day-1
Number of fillers 340 An
XA 0.00 mg COD·L-1
XH 0.82 mg COD·L-1
XA(％) 0.29 ％
XH(％) 99.71 ％
It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the examples given, those skilled in the art can modify the technical solution of the present invention as needed or equivalent substitutions without departing from the spirit and scope of the technical solution of the present invention.

Claims (10)

1. A method for detecting the content of active microorganisms in a biofilm of an MBBR system, the method comprising:
measuring the oxygen consumption respiration rate of the filler biofilm in the MBBR system;
and calculating the content of active microorganisms on the biological membrane by using an active sludge model.
2. The method of claim 1, wherein the specific method for determining the oxygen consumption respiration rate of the MBBR system biofilm comprises:
placing the filler in the MBBR system in a biological reaction system, and recording the dissolved oxygen concentration in the system along with time change in real time;
and drawing a dissolved oxygen-time curve, and calculating oxygen consumption respiration rate OURs.
3. The method of claim 2, wherein the biological reaction system comprises a biological reaction device, a stirring device, an aeration device, a dissolved oxygen measuring device, and a temperature measuring device;
the biological reaction device is a sealable transparent device and is used for placing the filler in the MBBR system;
the stirring device is arranged in the biological reaction device; preferably, the stirring device performs stirring in a stirring paddle mechanical stirring manner;
the aeration device is an aeration head connected with an aeration pump, and the aeration head extends into the bottom of the biological reaction device.
4. The method of claim 2, wherein the dissolved oxygen determination device is a portable dissolved oxygen meter; preferably, the dissolved oxygen measuring frequency is 1-2 min;
the temperature measuring device is a glass ball thermometer.
5. The method of claim 2, wherein the packing is pretreated prior to placing the packing in the bioreaction system.
6. The method of claim 2, wherein the pre-treatment method comprises rinsing the packing 3-5 times with clean water.
7. The method of claim 2, wherein the activated sludge model is ASM 1.
8. The method of claim 1, wherein the calculating the content of active microorganisms on the biofilm using the activated sludge model is performed by calculating the content and/or ratio of active microorganisms of autotrophic and heterotrophic microorganisms based on the oxygen consumption respiration rates OURs and the activated sludge model ASM 1.
9. A system for detecting the content of active microorganisms in a biological membrane of an MBBR system is characterized in that the system is a biological reaction system, and the biological reaction system comprises a biological reaction device, a stirring device, an aeration device, a dissolved oxygen measuring device and a temperature measuring device;
preferably, the biological reaction device is a sealable transparent device for placing the filler in the MBBR system therein;
preferably, the stirring device is arranged in the biological reaction device;
preferably, the aeration device is an aeration head connected with an aeration pump, and the aeration head extends into the bottom of the biological reaction device;
preferably, the temperature measuring device is a glass bulb thermometer.
10. Use of a method for detecting the content of active microorganisms of a biofilm of a MBBR system according to any of claims 1 to 8 and/or a system according to claim 9 in the determination of the content and/or ratio of active autotrophic and heterotrophic microorganisms of each chamber of a MBBR system.
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