CN111481397A - Hair follicle stem cell culture injection device and use method - Google Patents

Hair follicle stem cell culture injection device and use method Download PDF

Info

Publication number
CN111481397A
CN111481397A CN202010313391.8A CN202010313391A CN111481397A CN 111481397 A CN111481397 A CN 111481397A CN 202010313391 A CN202010313391 A CN 202010313391A CN 111481397 A CN111481397 A CN 111481397A
Authority
CN
China
Prior art keywords
fixing
block
fixed
base
groove
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010313391.8A
Other languages
Chinese (zh)
Inventor
赵焰
孙吉灵
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jinan Shengfatang Biotechnology Co ltd
Original Assignee
Jinan Shengfatang Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jinan Shengfatang Biotechnology Co ltd filed Critical Jinan Shengfatang Biotechnology Co ltd
Priority to CN202010313391.8A priority Critical patent/CN111481397A/en
Publication of CN111481397A publication Critical patent/CN111481397A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61GTRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
    • A61G13/00Operating tables; Auxiliary appliances therefor
    • A61G13/10Parts, details or accessories
    • A61G13/12Rests specially adapted therefor; Arrangements of patient-supporting surfaces
    • A61G13/1205Rests specially adapted therefor; Arrangements of patient-supporting surfaces for specific parts of the body
    • A61G13/121Head or neck
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/34Trocars; Puncturing needles
    • A61B17/3403Needle locating or guiding means
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/34Trocars; Puncturing needles
    • A61B17/3468Trocars; Puncturing needles for implanting or removing devices, e.g. prostheses, implants, seeds, wires
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/10Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges for stereotaxic surgery, e.g. frame-based stereotaxis
    • A61B90/14Fixators for body parts, e.g. skull clamps; Constructional details of fixators, e.g. pins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61GTRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
    • A61G15/00Operating chairs; Dental chairs; Accessories specially adapted therefor, e.g. work stands
    • A61G15/10Parts, details or accessories
    • A61G15/12Rests specially adapted therefor, e.g. for the head or feet
    • A61G15/125Head-rests
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00Surgical instruments, devices or methods, e.g. tourniquets
    • A61B2017/00743Type of operation; Specification of treatment sites
    • A61B2017/00747Dermatology
    • A61B2017/00752Hair removal or transplantation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/34Trocars; Puncturing needles
    • A61B17/3403Needle locating or guiding means
    • A61B2017/3405Needle locating or guiding means using mechanical guide means
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61GTRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
    • A61G2210/00Devices for specific treatment or diagnosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Neurosurgery (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Otolaryngology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of hair follicle stem cell injection, in particular to a hair follicle stem cell culture injection device and a using method thereof, wherein the device comprises a base, a mounting plate, a fixing structure, an adjusting structure, a transverse moving structure, a vertical moving structure, a rotating structure and a clamping structure; through fixed knot structure's setting, can fix human head, thereby can effectively prevent to take place the condition that the head rocked when injecting the hair follicle stem cell that will cultivate the completion to patient bald zone, the accuracy of injection has been guaranteed, through installation regulation structure between base and mounting panel, can adjust the angle of patient's head according to actual need, thereby can conveniently carry out the injection work of hair follicle stem cell more, cooperation through lateral shifting structure and vertical migration structure is used, can realize that the syringe carries out accurate injection to any one of human scalp, thereby can avoid taking place the condition of dislocation when carrying out the injection of hair follicle stem cell to human scalp, thereby can increase the efficiency of injection.

Description

Hair follicle stem cell culture injection device and use method
Technical Field
The invention relates to the technical field of hair follicle stem cell injection, in particular to a hair follicle stem cell culture injection device and a using method thereof.
Background
The hair follicle stem cell is one of the outer root sheath bulge of human hair follicles. The hair follicle stem cells belong to adult stem cells, are in a quiescent state in vivo, and show a surprising proliferation capacity under the action of in vitro culture. The research finds that the hair follicle stem cell has the multidirectional differentiation potential, can be differentiated into epidermis, hair follicle and sebaceous gland, and is involved in the process of healing skin wounds. Alopecia can be formed after the hair is abnormally fallen off, and inconvenience is brought to patients. The hair follicle of the normal hair of the patient is cultured, propagated and purified in vitro and then injected into the bald area of the patient, so as to generate new normal hair. At present, umbilical cord blood is adopted to separate stem cells, and the stem cells are injected in bald areas after separation culture.
At present when carrying out the injection of culture hair follicle stem cell to bald area, generally all be that medical personnel directly hold the syringe and carry out the injection work to bald area, but because when the injection, syringe and medical personnel's hand do not have any supporting role, and generally need many times and slowly inject when injecting hair follicle stem cell, and long-time holding action can lead to the condition that medical personnel's hand trembled, will cause the condition of injection deviation even damage scalp.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a hair follicle stem cell culture injection device and a using method thereof.
The technical scheme adopted by the invention for solving the technical problems is as follows: a hair follicle stem cell culture injection device comprises a base, an installation plate, a fixing structure, an adjusting structure, a transverse moving structure, a vertical moving structure, a rotating structure and a clamping structure, wherein the installation plate for fixing with a bed body or a chair is arranged at the bottom end of the base for supporting the head of a human body; the fixing structure for fixing the head of a human body is arranged at one end of the base, and the adjusting structure for adjusting the head angle of the human body is arranged between the base and the mounting plate; the transverse moving structure for controlling the transverse position of the syringe is arranged in the base, and the vertical moving structure for adjusting the vertical position of the syringe is arranged at one end of the base; the rotating structure used for adjusting the angle of the injector is arranged in the vertical moving structure, and the clamping structure used for clamping and fixing the injector is arranged at one end of the rotating structure.
Specifically, fixed knot constructs including fixed band, containing box, spool, gag lever post, gear and first spring the top of base is fixed the fixed band the fixed inside hollow of one end lateral wall of base the containing box the inside of containing box rotates to be connected with the spool, the other end of fixed band is fixed on the spool, just the fixed band winding is in on the spool the outside of spool is fixed the gear, just the gear with the containing box rotates to be connected the inside sliding connection of containing box has the gag lever post, just the gag lever post is close to the one end of gear is the triangle-shaped structure the gag lever post with the centre gripping is fixed between the containing box first spring.
Specifically, the adjusting structure comprises a first crank, a rotating shaft, an accommodating groove, a sliding block, a first screw rod, a limiting disc, a rotating sleeve and a connecting rod, the base and the mounting plate are rotatably connected through the rotating shaft, the accommodating groove is arranged in the mounting plate, the sliding groove is arranged in the mounting plate, the width of the accommodating groove is smaller than that of the sliding groove, the sliding block is slidably connected in the sliding groove, the first screw rod is rotatably connected in the mounting plate, the first screw rod penetrates through the sliding block and is in threaded connection with the sliding block, the limiting disc is fixed outside the first screw rod and is rotatably connected with the mounting plate, the rotating sleeve is fixed at the bottom end of the base, the connecting rod is connected between the sliding block and the rotating sleeve, and two ends of the connecting rod are respectively rotatably connected with the rotating sleeve and the sliding block, and the first crank is fixed at one end of the outer part of the first screw rod.
Specifically, the transverse moving structure comprises a fixing groove, a fixing block, a second screw rod, a sliding sleeve, a second crank, a fixing rod, a limiting groove and a limiting block, the fixing groove with a rectangular cross section is arranged in the base, the fixing block with a cuboid structure is connected in the fixing groove in a sliding manner, the second screw rod is rotatably connected in the base and penetrates through the fixing block and is in threaded connection with the fixing block, the fixing rod with a square cross section is fixed at one end of the second screw rod, the sliding sleeve with a rectangular structure is connected outside the fixing rod in a sliding manner, the sliding sleeve is connected with the base in a sliding manner, the second crank is fixed at one end of the sliding sleeve, the limiting groove is arranged in the fixing rod, and the limiting block is fixed in the sliding sleeve, and the limiting blocks are in sliding connection with the inner parts of the limiting grooves.
Specifically, the vertical moving structure comprises an upright column, a moving groove, a moving block and a locking screw, the upright column is fixed at one end of the fixed block, the moving groove is arranged in the upright column and is of a cross-shaped structure, the moving block is slidably connected in the moving groove, the locking screw is connected to one end of the moving block in a threaded manner, and the maximum external diameter of the locking screw is greater than the external width of the moving groove.
Specifically, the rotating structure comprises a screw rod, a connecting block, a second spring and a locking nut, the screw rod is connected inside the moving block in a sliding mode and penetrates through the moving block, the connecting block is fixed to one end of the screw rod, the second spring is clamped between the connecting block and the moving block and penetrates through the second spring, the locking nut is connected to the outer portion of the screw rod in a threaded mode, and the minimum outer diameter of the locking nut is smaller than the width of the moving block.
Specifically, the clamping structure includes solid fixed ring, rubber circle, clamp splice and locking screw, is the loop configuration gu fixed ring is fixed in the connecting block deviates from the one end of screw rod gu fixed ring's inside is spliced the rubber circle gu fixed ring's inside sliding connection has and is fan-shaped structure the clamp splice the one end of clamp splice is rotated and is connected with locking screw, just locking screw with gu fixed ring threaded connection.
Specifically, the using method of the hair follicle stem cell culture injection device comprises the following steps:
s1: firstly, fixing the mounting plate on a bed body or an arm-chair, enabling the head of a patient to lean against the inside of a groove in a base, and then rotating a fixing structure to fix the head of the patient;
s2: then quantitatively extracting the cultured hair follicle stem cells by using an injector, and then clamping and fixing the injector with the extracted hair follicle stem cells by using a clamping structure;
s3: then according to the position of the hair-off area that needs the injection, adjust lateral shifting structure, vertical migration structure and rotation structure's position, treat that the position is confirmed after finishing, can also adjust the angle between base and the mounting panel through adjusting according to operator's specific operating conditions, can loosen clamping structure a little at last and make the syringe just can slide, can carry out injection work at last.
The invention has the beneficial effects that:
(1) the hair follicle stem cell culture injection device and the use method can fix the head of a human body through the arrangement of the fixing structure, thereby effectively preventing the situation that the head shakes when the cultured hair follicle stem cells are injected to a bald area of a patient, ensuring the injection accuracy, namely when the hair follicle stem cells which are successfully cultured are required to be injected to the bald area of the patient, firstly, the mounting plate needs to be fixed on a bed body or a chair, then, the head of the patient leans against a base, then, the fixing strap passes through the upper part of the forehead of the human body, then, the reel is rotated, as the other end of the fixing strap is fixed on the outer part of the reel, the fixing strap can be wound on the reel when the reel is rotated, as the gear is fixed on the outer part of the reel, the limiting rod is connected in the storage box in a sliding way, and one end of the limiting rod, which is close to the gear, is of a triangular, under the elastic force effect of first spring, the gag lever post remains the limiting displacement to the gear all the time, makes the spool only can rotate clockwise, has realized promptly when rotating the spool that the condition of taut and can not take place automatic resilience of fixed band has guaranteed the fixed action to patient's head, and when not needing to fix, only need with the gag lever post pull up can, convenient and fast, and fixed effectual.
(2) The invention relates to a hair follicle stem cell culture injection device and a using method thereof, wherein an adjusting structure is arranged between a base and a mounting plate, and the angle of the head of a patient can be adjusted according to actual needs, so that the injection work of the hair follicle stem cells can be more convenient, namely, the base and the mounting plate can be ensured to be adjustable because the base and the mounting plate can be rotatably connected through a rotating shaft, and the rotating angle of the base and the mounting plate can be adjusted because the mounting plate is internally provided with a containing groove and a sliding groove for a sliding block to slide, therefore, when the base and the mounting plate are covered, a connecting rod rotatably connected between a rotating sleeve and the sliding block can be completely contained, because the mounting plate is rotatably connected with a first screw rod in the inner part, and the first screw rod penetrates through the sliding block and is in threaded connection with the sliding block, when a first crank is rotated in the outer part, the, just can make the slider slide in the inside of spout, when the slider slides to the one end of pivot, just can promote the connecting rod with base jack-up to can realize the angle change of base, thereby can be convenient for the injection work of syringe according to actual conditions, and the operation of being convenient for.
(3) The invention relates to a hair follicle stem cell culture injection device and a using method thereof, which can realize that an injector can accurately inject any point of human scalp through the matching use of a transverse moving structure and a vertical moving structure, thereby avoiding the dislocation condition when injecting hair follicle stem cells to the human scalp, and increasing the injection efficiency, namely, as a fixed block is connected in a sliding way in a fixed groove, and a second screw rod which is rotationally connected in a base penetrates through the fixed block and is in threaded connection with the fixed block, when the second screw rod is rotated, the fixed block can slide in the fixed groove, thereby changing the transverse any position of the injector, being convenient for realizing accurate injection, because a fixed rod which is in a cuboid structure is fixed at one end of the second screw rod, and a sliding sleeve which is in a cuboid structure is connected outside the fixed rod in a sliding way, the limiting groove is formed in the fixing rod, the limiting block is fixed in the sliding sleeve, after the sliding sleeve slides out of the base, the sliding sleeve is not limited by the base any more at the moment, the second crank is rotated at the moment to rotate the second lead screw, the transverse movement of the fixing block is realized, when the sliding sleeve needs to be fixed, the sliding sleeve only needs to be inserted into the base, the injection device is convenient and practical, the stand column is fixed to one end of the fixing block and can be used for supporting the injection device, the moving groove in a cross-shaped structure is formed in the stand column, the moving block is connected to the inside of the moving groove in a sliding mode, through the movement of the moving block, the vertical optional position of the injection device can be fixed, accurate injection can be realized, when the moving block needs to be fixed, only the locking screw needs to be rotated, convenience and rapid.
(4) The hair follicle stem cell culture injection device and the use method can quickly install and fix injectors of different types by the arrangement of the clamping structure, avoid the situation that an operator has manual injection deviation during injection, and can realize the rotation adjusting function of the injector by matching with the arrangement of the rotating structure, thereby not only improving the injection accuracy, but also facilitating the operation of the operator according to the actual situation and improving the injection efficiency, namely, when the hair follicle stem cells are required to be injected to a hair loss area, the cultured hair follicle stem cells need to be extracted by the injector firstly, then the injector is inserted into the fixed ring, then the locking screw rod is rotated, as the locking screw rod which is connected with the fixed ring by screw threads is rotationally connected with the clamp block which is slidably connected with the fixed ring, the fixing effect of the clamp block on the injector can be realized when the locking screw rod is rotated, convenient and fast, and fixed firm, avoid taking place the condition of rocking when the injection, because the connecting block that is used for fixed ring is fixed with sliding connection in the inside screw rod of movable block, when rotating threaded connection in the outside lock nut of screw rod, can realize the conflict locking effect of connecting block and movable block, realize the fixed of movable block, when loosening, still can rotate the screw rod, realize the rotation regulation of syringe, the operating personnel of being convenient for carries out injection operation and accurate injection to improve injection efficiency greatly.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a structural diagram illustrating the overall structure of a preferred embodiment of the injection device for culturing hair follicle stem cells provided by the present invention;
FIG. 2 is a schematic structural view of the fixing structure shown in FIG. 1;
FIG. 3 is a schematic view showing a connection structure of the reel and the gear shown in FIG. 1;
FIG. 4 is a schematic view of the attachment of the mounting plate and adjustment structure shown in FIG. 1;
FIG. 5 is a schematic view of the connection structure of the base and the lateral shifting structure shown in FIG. 1;
FIG. 6 is an enlarged view of portion A of FIG. 5;
FIG. 7 is a schematic view of a connection structure of the rotating structure and the clamping structure shown in FIG. 1;
fig. 8 is a flow chart of the method of using the hair follicle stem cell culture injection device provided by the invention.
In the figure: 1. a base, 2, a mounting plate, 3, a fixing structure, 31, a fixing band, 32, a containing box, 33, a scroll, 34, a limiting rod, 35, a gear, 36, a first spring, 4, an adjusting structure, 41, a first crank, 42, a rotating shaft, 43, a containing groove, 44, a sliding groove, 45, a sliding block, 46, a first screw rod, 47, a limiting disc, 48, a rotating sleeve, 49, a connecting rod, 5, a transverse moving structure, 51, a fixing groove, 52, a fixing block, 53, a second screw rod, 54, a sliding sleeve, 55, a second crank, 56, a fixing rod, 57, a limiting groove, 58, a limiting block, 6, a vertical moving structure, 61, a column, 62, a moving groove, 63, a moving block, 64, a locking screw, 7, a rotating structure, 71, a screw rod, 72, a connecting block, 73, a second spring, 74, a locking nut, 8, a clamping structure, 81, a fixing ring, 82, a rubber ring, 83 and a clamping block, 84. and locking the screw rod.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1-7, the injection device for culturing hair follicle stem cells comprises a base 1, a mounting plate 2, a fixing structure 3, an adjusting structure 4, a transverse moving structure 5, a vertical moving structure 6, a rotating structure 7 and a clamping structure 8, wherein the mounting plate 2 for fixing with a bed or a chair is arranged at the bottom end of the base 1 for supporting the head of a human body; the fixing structure 3 for fixing the head of a human body is arranged at one end of the base 1, and the adjusting structure 4 for adjusting the head angle of the human body is arranged between the base 1 and the mounting plate 2; the transverse moving structure 5 for controlling the transverse position of the syringe is arranged in the base 1, and the vertical moving structure 6 for adjusting the vertical position of the syringe is arranged at one end of the base 1; the rotating structure 7 for adjusting the angle of the syringe is arranged in the vertical moving structure 6, and the clamping structure 8 for clamping and fixing the syringe is arranged at one end of the rotating structure 7.
Specifically, the fixing structure 3 includes a fixing strap 31, a storage box 32, a reel 33, a limiting rod 34, a gear 35 and a first spring 36, the top end of the base 1 is fixed with the fixing strap 31, the storage box 32 with a side wall at one end of the base 1 fixed with the hollow inside is connected with the reel 33 in the storage box 32, the other end of the fixing strap 31 is fixed on the reel 33, the fixing strap 31 is wound on the reel 33, the gear 35 is fixed outside the reel 33, the gear 35 is connected with the storage box 32 in a rotating manner, the limiting rod 34 is connected with the storage box 32 in a sliding manner, one end of the limiting rod 34 close to the gear 35 is in a triangular structure, the first spring 36 is clamped and fixed between the limiting rod 34 and the storage box 32, when the cultured hair follicle stem cells are required to be injected into a bald area of a patient, firstly, the mounting plate 2 needs to be fixed on a bed or a chair, then the head of a patient leans against the base 1, then the fixing belt 31 passes through the forehead of the human body, then the reel 33 is rotated, because the other end of the fixing belt 31 is fixed outside the reel 33, the fixing belt 31 can be wound on the reel 33 when the reel 33 is rotated, because the gear 35 is fixed outside the reel 33, the limiting rod 34 is connected inside the storage box 32 in a sliding manner, and one end of the limiting rod 34 close to the gear 35 is in a triangular structure, under the elastic force of the first spring 36, the limiting rod 34 always keeps the limiting effect on the gear 35, so that the reel 33 can only rotate clockwise, namely when the reel 33 is rotated, the situation that the fixing belt 31 is tensioned and cannot automatically rebound is achieved, the fixing effect on the head of a patient is guaranteed, and when the fixing is not needed, the limiting rod 34 only needs to be pulled up, so that the fixing device is convenient and fast to use and good in fixing effect.
Specifically, the adjusting structure 4 includes a first crank 41, a rotating shaft 42, a containing groove 43, a sliding groove 44, a sliding block 45, a first screw rod 46, a limiting disc 47, a rotating sleeve 48 and a connecting rod 49, the base 1 and the mounting plate 2 are connected by the rotating shaft 42, the containing groove 43 is arranged inside the mounting plate 2, the sliding groove 44 is arranged inside the mounting plate 2, the width of the containing groove 43 is smaller than the width of the sliding groove 44, the sliding block 45 is connected inside the sliding groove 44, the first screw rod 46 is connected inside the mounting plate 2 in a rotating manner, the first screw rod 46 penetrates through the sliding block 45 and is in threaded connection with the sliding block 45, the limiting disc 47 is fixed outside the first screw rod 46, the limiting disc 47 is connected with the mounting plate 2 in a rotating manner, the rotating sleeve 48 is fixed at the bottom end of the base 1, the connecting rod 49 is connected between the sliding block 45 and the rotating sleeve 48, two ends of the connecting rod 49 are respectively rotatably connected with the rotating sleeve 48 and the sliding block 45, the first crank 41 is fixed at one end outside the first screw rod 46, the base 1 and the mounting plate 2 can be rotatably connected through the rotating shaft 42, so that the base 1 and the mounting plate 2 can be ensured to be adjustable in rotation angle, the accommodating groove 43 and the sliding groove 44 for the sliding block 45 are arranged inside the mounting plate 2, so that when the base 1 and the mounting plate 2 are covered, the connecting rod 49 rotatably connected between the rotating sleeve 48 and the sliding block 45 can be completely accommodated, the first screw rod 46 is rotatably connected inside the mounting plate 2, and the first screw rod 46 penetrates through the sliding block 45 and is in threaded connection with the sliding block 45, therefore, when the first crank 41 is rotated externally, the first lead screw 46 can be rotated, the slider 45 can be slid inside the sliding groove 44 by the rotation of the first lead screw 46, and when the slider 45 slides to one end of the rotating shaft 42, the connecting rod 49 can be pushed to jack up the base 1, so that the angle change of the base 1 can be realized, the injection work of the injector can be facilitated according to actual conditions, and the operation is facilitated.
Specifically, the transverse moving structure 5 includes a fixing groove 51, a fixing block 52, a second screw 53, a sliding sleeve 54, a second crank 55, a fixing rod 56, a limiting groove 57 and a limiting block 58, the fixing groove 51 with a rectangular cross section is disposed inside the base 1, the fixing block 52 with a rectangular parallelepiped structure is slidably connected inside the fixing groove 51, the second screw 53 is rotatably connected inside the base 1, the second screw 53 penetrates through the fixing block 52 and is in threaded connection with the fixing block 52, the fixing rod 56 with a square cross section is fixed at one end of the second screw 53, the sliding sleeve 54 with a rectangular outer structure is slidably connected outside the fixing rod 56, the sliding sleeve 54 is slidably connected with the base 1, the second crank 55 is fixed at one end of the sliding sleeve 54, the limiting groove 57 is disposed inside the fixing rod 56, the limit block 58 is fixed inside the sliding sleeve 54, and the limit block 58 is slidably connected inside the limit groove 57, because the fixed block 52 is slidably connected inside the fixed groove 51, and the second lead screw 53 rotatably connected inside the base 1 penetrates through the fixed block 52 and is in threaded connection with the fixed block 52, when the second lead screw 53 is rotated, the fixed block 52 can slide inside the fixed groove 51, so that any horizontal position of the injector can be changed, and accurate injection is facilitated, because the fixed rod 56 with a cuboid structure is fixed at one end of the second lead screw 53, the sliding sleeve 54 with a cuboid structure is slidably connected outside the fixed rod 56, the limit groove 57 is arranged on the fixed rod 56, and the limit block 58 is fixed inside the sliding sleeve 54, when will the sliding sleeve 54 follows the inside roll-off of base 1 back, at this moment the sliding sleeve 54 no longer receives the limiting displacement of base 1 rotates this moment second crank 55 can realize the rotation of second lead screw 53 realizes the lateral shifting of fixed block 52, when needs are fixed, only need with the sliding sleeve 54 insert the inside of base 1 can, convenient and practical.
Specifically, the vertical moving structure 6 includes an upright post 61, a moving groove 62, a moving block 63 and a locking screw 64, the upright post 61 is fixed at one end of the fixed block 52, the moving groove 62 is arranged inside the upright post 61, the moving groove 62 is in a cross-shaped structure, the moving block 63 is slidably connected inside the moving groove 62, the locking screw 64 is threadedly connected at one end of the moving block 63, the maximum outer diameter of the locking screw 64 is larger than the outer width of the moving groove 62, the upright post 61 fixed at one end of the fixed block 52 can be used for supporting the injector, since the moving groove 62 in the cross-shaped structure is arranged inside the upright post 61, and the moving block 63 is slidably connected inside the moving groove 62, any vertical position of the injector can be fixed by the movement of the moving block 63, therefore, accurate injection can be achieved, and when the moving block 63 needs to be fixed, only the locking screw 64 needs to be rotated, so that the injection device is convenient and rapid to use and accurate in positioning.
Specifically, the rotating structure 7 includes a screw 71, a connecting block 72, a second spring 73 and a locking nut 74, the screw 71 is slidably connected to the inside of the moving block 63, the screw 71 penetrates through the moving block 63, the connecting block 72 is fixed at one end of the screw 71, the second spring 73 is clamped between the connecting block 72 and the moving block 63, the screw 71 penetrates through the second spring 73, the locking nut 74 is connected to the outside of the screw 71 through a thread, and the minimum outside diameter of the locking nut 74 is smaller than the width of the moving block 63, since the connecting block for fixing the fixing ring is fixed to the screw 71 slidably connected to the inside of the moving block, when the locking nut 74 threadedly connected to the outside of the screw 71 is rotated, the locking action between the connecting block 72 and the moving block 63 can be achieved, the fixing of the moving block 63 is realized, and when the moving block is loosened, the screw rod 71 can be rotated, so that the rotation adjustment of the injector is realized, the injection operation and the accurate injection are convenient for operators, and the injection efficiency is greatly improved.
Specifically, clamping structure 8 is including solid fixed ring 81, rubber circle 82, clamp splice 83 and locking screw 84, is the loop configuration gu fixed ring 81 is fixed in the connecting block 72 deviates from the one end of screw 71 gu fixed ring 81's inside is glued joint rubber circle 82 gu fixed ring 81's inside sliding connection has and is fan-shaped structure clamp splice 83 the one end of clamp splice 83 is rotated and is connected with locking screw 84, just locking screw 84 with gu fixed ring 81 threaded connection, when needing to inject hair follicle stem cell to alopecia area, need extract the hair follicle stem cell that will cultivate with the syringe earlier, then insert the syringe gu fixed ring 81's inside, then rotate locking screw 84, because threaded connection in gu fixed ring 81 locking screw 84 with sliding connection in gu fixed ring 81 clamp splice 83 rotates and connects, therefore, when the locking screw 84 is rotated, the fixing effect of the clamping block 83 on the injector can be realized, the fixing is convenient and fast, the fixing is firm, and the shaking condition during injection is avoided.
Specifically, referring to fig. 8, fig. 8 is a flowchart of a method for using the injection device for culturing hair follicle stem cells provided by the present invention, wherein the method for using the injection device for culturing hair follicle stem cells comprises the following steps:
s1: firstly, fixing the mounting plate on a bed body or an arm-chair, enabling the head of a patient to lean against the inside of a groove in a base, and then rotating a fixing structure to fix the head of the patient;
s2: then quantitatively extracting the cultured hair follicle stem cells by using an injector, and then clamping and fixing the injector with the extracted hair follicle stem cells by using a clamping structure;
s3: then according to the position of the hair-off area that needs the injection, adjust lateral shifting structure, vertical migration structure and rotation structure's position, treat that the position is confirmed after finishing, can also adjust the angle between base and the mounting panel through adjusting according to operator's specific operating conditions, can loosen clamping structure a little at last and make the syringe just can slide, can carry out injection work at last.
When the hair follicle stem cells which are successfully cultured are required to be injected into a baldness area of a patient, the mounting plate 2 is firstly required to be fixed on a bed body or a chair, then the head of the patient is leaned on the base 1, then the fixing belt 31 passes through the forehead of the human body, then the reel 33 is rotated, the fixing belt 31 can be wound on the reel 33 when the reel 33 is rotated because the other end of the fixing belt 31 is fixed on the outer part of the reel 33, the limiting rod 34 is connected in the containing box 32 in a sliding manner because the gear 35 is fixed on the outer part of the reel 33, and one end of the limiting rod 34 close to the gear 35 is in a triangular structure, so under the elastic force of the first spring 36, the limiting rod 34 always keeps limiting action on the gear 35, the reel 33 can only rotate clockwise, namely, when the reel 33 is rotated, the fixing belt 31 is tensioned without automatic rebounding, the fixing effect on the head of a patient is ensured, and when the fixing is not needed, the limiting rod 34 only needs to be pulled up, so that the fixing is convenient and rapid, and the fixing effect is good; when the hair follicle stem cells are required to be injected to a hair loss area, the cultured hair follicle stem cells are required to be extracted by an injector firstly, then the injector is inserted into the fixing ring 81, and then the locking screw 84 is rotated, because the locking screw 84 in threaded connection with the fixing ring 81 is in rotating connection with the clamping block 83 in sliding connection with the fixing ring 81, the fixing effect of the clamping block 83 on the injector can be realized when the locking screw 84 is rotated, the operation is convenient and fast, the fixation is firm, and the situation of shaking during injection is avoided; because the connecting block for fixing the fixing ring is fixed with the screw 71 which is slidably connected inside the moving block, when the locking nut 74 which is in threaded connection with the outside of the screw 71 is rotated, the abutting locking effect of the connecting block 72 and the moving block 63 can be realized, the fixing of the moving block 63 is realized, and when the locking nut 74 is loosened, the screw 71 can be rotated, the rotation adjustment of the injector is realized, so that the injection operation and the accurate injection can be conveniently performed by an operator, and the injection efficiency is greatly improved; since the base 1 and the mounting plate 2 are rotatably connected by the rotating shaft 42, it is ensured that the rotation angle of the base 1 and the mounting plate 2 is adjustable, since the receiving groove 43 and the sliding groove 44 for the sliding of the sliding block 45 are provided in the mounting plate 2, when the base 1 and the mounting plate 2 are covered, the connecting rod 49 rotatably connected between the rotating sleeve 48 and the sliding block 45 can be completely received, since the first screw 46 is rotatably connected in the mounting plate 2, and the first screw 46 penetrates the sliding block 45 and is screwed with the sliding block 45, when the first handle 41 is externally rotated, the rotation of the first screw 46 can be realized, and the sliding block 45 can be slid in the sliding groove 44 by the rotation of the first screw 46, when the sliding block 45 slides to one end of the rotating shaft 42, the connecting rod 49 can be pushed to jack up the base 1, so that the angle change of the base 1 can be realized, the injection work of the injector can be facilitated according to actual conditions, and the operation is facilitated; because the fixed block 52 is connected to the inside of the fixed slot 51 in a sliding manner, and the second lead screw 53 rotatably connected to the inside of the base 1 penetrates through the fixed block 52 and is in threaded connection with the fixed block 52, when the second lead screw 53 is rotated, the fixed block 52 can slide in the inside of the fixed slot 51, so that any horizontal position of the injector can be changed, and accurate injection can be realized, because the fixed rod 56 in a cuboid structure is fixed at one end of the second lead screw 53, the sliding sleeve 54 in a cuboid structure is slidably connected to the outside of the fixed rod 56, the limit groove 57 is arranged on the fixed rod 56, the limit block 58 is fixed in the sliding sleeve 54, and after the sliding sleeve 54 slides out from the inside of the base 1, the sliding sleeve 54 is not subjected to the limit action of the base 1 any more, at the moment, the second crank 55 is rotated to rotate the second screw 53 and realize the transverse movement of the fixed block 52, and when the fixed block needs to be fixed, the sliding sleeve 54 only needs to be inserted into the base 1, so that the device is convenient and practical; the upright post 61 fixed at one end of the fixed block 52 can be used for supporting an injector, the movable groove 62 in a cross-shaped structure is arranged in the upright post 61, the movable block 63 is connected in the movable groove 62 in a sliding manner, and the injector can be fixed at any vertical position by moving the movable block 63, so that accurate injection can be realized, and when the movable block 63 needs to be fixed, only the locking screw 64 needs to be rotated, so that the injector is convenient and rapid and is accurate in positioning.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (8)

1. A hair follicle stem cell culture injection device is characterized by comprising a base (1), a mounting plate (2), a fixing structure (3), an adjusting structure (4), a transverse moving structure (5), a vertical moving structure (6), a rotating structure (7) and a clamping structure (8), wherein the mounting plate (2) for fixing with a bed body or a chair is arranged at the bottom end of the base (1) for supporting the head of a human body; the fixing structure (3) for fixing the head of a human body is arranged at one end of the base (1), and the adjusting structure (4) for adjusting the head angle of the human body is arranged between the base (1) and the mounting plate (2); the transverse moving structure (5) for controlling the transverse position of the syringe is arranged in the base (1), and the vertical moving structure (6) for adjusting the vertical position of the syringe is arranged at one end of the base (1); the rotating structure (7) used for adjusting the angle of the syringe is arranged in the vertical moving structure (6), and the clamping structure (8) used for clamping and fixing the syringe is arranged at one end of the rotating structure (7).
2. The hair follicle stem cell culture injection device of claim 1, which is characterized in that: the fixing structure (3) comprises a fixing belt (31), a containing box (32), a scroll (33), a limiting rod (34), a gear (35) and a first spring (36), the fixing belt (31) is fixed at the top end of the base (1), the containing box (32) which is hollow inside is fixed on one end side wall of the base (1), the scroll (33) is rotatably connected inside the containing box (32), the other end of the fixing belt (31) is fixed on the scroll (33), the fixing belt (31) is wound on the scroll (33), the gear (35) is fixed outside the scroll (33), the gear (35) is rotatably connected with the containing box (32), the limiting rod (34) is slidably connected inside the containing box (32), and one end, close to the gear (35), of the limiting rod (34) is of a triangular structure, the first spring (36) is clamped and fixed between the limiting rod (34) and the containing box (32).
3. The hair follicle stem cell culture injection device of claim 1, which is characterized in that: the adjusting structure (4) comprises a first crank (41), a rotating shaft (42), a containing groove (43), a sliding groove (44), a sliding block (45), a first screw rod (46), a limiting disc (47), a rotating sleeve (48) and a connecting rod (49), wherein the base (1) and the mounting plate (2) are connected in a rotating mode through the rotating shaft (42), the containing groove (43) is arranged inside the mounting plate (2), the sliding groove (44) is arranged inside the mounting plate (2), the width of the containing groove (43) is smaller than that of the sliding groove (44), the sliding block (45) is connected inside the sliding groove (44), the first screw rod (46) is connected inside the mounting plate (2) in a rotating mode, the first screw rod (46) penetrates through the sliding block (45) and is in threaded connection with the sliding block (45), the limiting disc (47) is fixed outside the first screw rod (46), and spacing dish (47) with mounting panel (2) rotate to be connected the bottom mounting of base (1) commentaries on classics cover (48) slider (45) with connect between commentaries on classics cover (48) connecting rod (49), just the both ends of connecting rod (49) respectively with change cover (48) with slider (45) rotate to be connected the outside one end of first lead screw (46) is fixed first crank (41).
4. The hair follicle stem cell culture injection device of claim 1, which is characterized in that: the transverse moving structure (5) comprises a fixing groove (51), a fixing block (52), a second screw rod (53), a sliding sleeve (54), a second crank (55), a fixing rod (56), a limiting groove (57) and a limiting block (58), wherein the fixing groove (51) with a rectangular cross section is arranged in the base (1), the fixing block (52) with a cuboid structure is connected in the fixing groove (51) in a sliding manner, the second screw rod (53) is rotatably connected in the base (1), the second screw rod (53) penetrates through the fixing block (52) and is in threaded connection with the fixing block (52), the fixing rod (56) with a square cross section is fixed at one end of the second screw rod (53), the sliding sleeve (54) with a rectangular outer portion is connected in a sliding manner outside the fixing rod (56), the sliding sleeve (54) is in sliding connection with the base (1), the second crank (55) is fixed at one end of the sliding sleeve (54), the limiting groove (57) is formed in the fixing rod (56), the limiting block (58) is fixed in the sliding sleeve (54), and the limiting block (58) is in sliding connection with the limiting groove (57).
5. The hair follicle stem cell culture injection device of claim 4, which is characterized in that: the vertical moving structure (6) comprises an upright post (61), a moving groove (62), a moving block (63) and a locking screw (64), the upright post (61) is fixed at one end of the fixed block (52), the moving groove (62) is arranged inside the upright post (61), the moving groove (62) is of a cross-shaped structure, the moving block (63) is connected inside the moving groove (62) in a sliding mode, the locking screw (64) is connected to one end of the moving block (63) in a threaded mode, and the maximum outer diameter of the locking screw (64) is larger than the outer width of the moving groove (62).
6. The hair follicle stem cell culture injection device of claim 5, which is characterized in that: the rotating structure (7) comprises a screw rod (71), a connecting block (72), a second spring (73) and a locking nut (74), the screw rod (71) is connected to the inside of the moving block (63) in a sliding mode, the screw rod (71) penetrates through the moving block (63), the connecting block (72) is fixed to one end of the screw rod (71), the second spring (73) is clamped between the connecting block (72) and the moving block (63), the screw rod (71) penetrates through the second spring (73), the locking nut (74) is connected to the outer portion of the screw rod (71) in a threaded mode, and the minimum outer diameter of the locking nut (74) is smaller than the width of the moving block (63).
7. The hair follicle stem cell culture injection device of claim 6, which is characterized in that: clamping structure (8) are loop configuration including solid fixed ring (81), rubber ring (82), clamp splice (83) and locking screw (84) gu fixed ring (81) are fixed in connecting block (72) deviate from the one end of screw rod (71) the inside of solid fixed ring (81) is glued rubber ring (82) the inside sliding connection of solid fixed ring (81) has and is fan-shaped structure clamp splice (83) the one end of clamp splice (83) is rotated and is connected with locking screw (84), just locking screw (84) with gu fixed ring (81) threaded connection.
8. The use method of the hair follicle stem cell culture injection device according to claims 1-7, characterized in that: the using method comprises the following steps:
s1: firstly, fixing the mounting plate on a bed body or an arm-chair, enabling the head of a patient to lean against the inside of a groove in a base, and then rotating a fixing structure to fix the head of the patient;
s2: then quantitatively extracting the cultured hair follicle stem cells by using an injector, and then clamping and fixing the injector with the extracted hair follicle stem cells by using a clamping structure;
s3: then according to the position of the hair-off area that needs the injection, adjust lateral shifting structure, vertical migration structure and rotation structure's position, treat that the position is confirmed after finishing, can also adjust the angle between base and the mounting panel through adjusting according to operator's specific operating conditions, can loosen clamping structure a little at last and make the syringe just can slide, can carry out injection work at last.
CN202010313391.8A 2020-04-20 2020-04-20 Hair follicle stem cell culture injection device and use method Pending CN111481397A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010313391.8A CN111481397A (en) 2020-04-20 2020-04-20 Hair follicle stem cell culture injection device and use method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010313391.8A CN111481397A (en) 2020-04-20 2020-04-20 Hair follicle stem cell culture injection device and use method

Publications (1)

Publication Number Publication Date
CN111481397A true CN111481397A (en) 2020-08-04

Family

ID=71789705

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010313391.8A Pending CN111481397A (en) 2020-04-20 2020-04-20 Hair follicle stem cell culture injection device and use method

Country Status (1)

Country Link
CN (1) CN111481397A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112006880A (en) * 2020-09-09 2020-12-01 新乡医学院第一附属医院 Fixing device for orthopedic anesthesia nursing
CN112587177A (en) * 2020-12-09 2021-04-02 北京世通瑞泰医疗器械有限公司 Temporomandibular arthroscope instrument matched with temporomandibular arthroscope for use

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107080592A (en) * 2017-06-05 2017-08-22 郑州大学第附属医院 A kind of ganglioside GM_3 incidence fixing device
CN107335124A (en) * 2017-08-10 2017-11-10 泉州市惠安闽投商贸有限公司 A kind of epidural anaesthesia device
WO2018157882A1 (en) * 2017-03-01 2018-09-07 Hubert Noras Biopsy needle guide having two-stage fixation
CN108814759A (en) * 2018-07-03 2018-11-16 广东医诺生物科技有限公司 A kind of fixing device for experimental animal
CN109646088A (en) * 2019-01-15 2019-04-19 高密市人民医院 A kind of surgical operation lumbar anesthesia auxiliary locator
CN109700484A (en) * 2018-12-24 2019-05-03 吴道诩 A kind of Nephrology dept.'s biopsy puncture device
CN110522960A (en) * 2019-05-29 2019-12-03 王华丽 A kind of portable flusher for bleeding wounds nursing

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018157882A1 (en) * 2017-03-01 2018-09-07 Hubert Noras Biopsy needle guide having two-stage fixation
CN107080592A (en) * 2017-06-05 2017-08-22 郑州大学第附属医院 A kind of ganglioside GM_3 incidence fixing device
CN107335124A (en) * 2017-08-10 2017-11-10 泉州市惠安闽投商贸有限公司 A kind of epidural anaesthesia device
CN108814759A (en) * 2018-07-03 2018-11-16 广东医诺生物科技有限公司 A kind of fixing device for experimental animal
CN109700484A (en) * 2018-12-24 2019-05-03 吴道诩 A kind of Nephrology dept.'s biopsy puncture device
CN109646088A (en) * 2019-01-15 2019-04-19 高密市人民医院 A kind of surgical operation lumbar anesthesia auxiliary locator
CN110522960A (en) * 2019-05-29 2019-12-03 王华丽 A kind of portable flusher for bleeding wounds nursing

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112006880A (en) * 2020-09-09 2020-12-01 新乡医学院第一附属医院 Fixing device for orthopedic anesthesia nursing
CN112006880B (en) * 2020-09-09 2022-07-01 新乡医学院第一附属医院 Fixing device for orthopedic anesthesia nursing
CN112587177A (en) * 2020-12-09 2021-04-02 北京世通瑞泰医疗器械有限公司 Temporomandibular arthroscope instrument matched with temporomandibular arthroscope for use

Similar Documents

Publication Publication Date Title
CN111481397A (en) Hair follicle stem cell culture injection device and use method
CN209565929U (en) A kind of sewing needle grinding apparatus
CN112716541B (en) Cervical vertebra anterior surgery gap retractor
CN208756441U (en) A kind of auxiliary lift leg adjustable speed medical optical fiber automatic returning device
CN215606131U (en) Epidural anesthesia positioning auxiliary device
CN213190192U (en) Laparoscope supporting device for single-port laparoscopic surgery
CN209644973U (en) A kind of mastoid process dilator
CN211534577U (en) Fixed drag hook for thyroid operation
CN208874929U (en) A kind of municipal administration gardens vine plant climbing device
CN208725774U (en) A kind of acra skin drawing device
CN209966462U (en) Posterior approach hip joint replacement operation auxiliary device
CN112715201A (en) Combined cultivation frame for pineapple flower forcing cultivation and implementation method thereof
CN209018929U (en) For the probe fixture in percutaneous irreversible electroporation ablation
CN218739087U (en) Hair follicle micro-needle hair-planting device
CN215652464U (en) Intelligent wearing hair growth instrument with massage effect
CN219981637U (en) Suspension type cultivation device based on wisdom agricultural is used
CN219742993U (en) Apple muscle reset beauty pulling wire
CN213044469U (en) A cultivation shed frame of taking shelter from rain for grape high grafting is traded kind
CN219250320U (en) Hand-twisting type pressing hemostasis device matched with belt
CN218184368U (en) Afforestation plant protection machanism
CN216221716U (en) Adjustable scalpel for implant tooth
CN110575214A (en) Skin stretching device
CN113632666B (en) Tomato is planted and is used tendril fixing device
KR200424303Y1 (en) An artificial hair for transplanting to skin
CN216874027U (en) Automatic trimming device of photinia fraseri

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20200804

RJ01 Rejection of invention patent application after publication