CN111471576B - From deep sea microorganism normal position culture apparatus of taking pressure compensation - Google Patents
From deep sea microorganism normal position culture apparatus of taking pressure compensation Download PDFInfo
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- CN111471576B CN111471576B CN202010440118.1A CN202010440118A CN111471576B CN 111471576 B CN111471576 B CN 111471576B CN 202010440118 A CN202010440118 A CN 202010440118A CN 111471576 B CN111471576 B CN 111471576B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention discloses a deep sea microorganism in-situ culture device with pressure compensation, which comprises a culture tank, a culture tank end cover, a culture tank shell, a connecting body, a motor cylinder end cover, a pressure compensation device, a watertight connector, a stepping motor, a coupler, a trapezoidal screw rod, a sealing ring, a hollow threaded rod, a plug and a fixed support, wherein the culture tank and the culture tank end cover are connected through threads; the cultivation tank and the cultivation tank end cover are movably arranged in a cultivation tank shell, the cultivation tank shell is connected with a connector through a fixing support, the connector is connected with a motor cylinder, the inner wall of a hollow threaded rod is provided with trapezoidal threads and is meshed with a trapezoidal screw rod, and the stepping motor is connected with the trapezoidal screw rod through a coupler. The invention is arranged on a deep sea lander, and the in-situ enrichment culture and recovery are carried out on deep sea microorganisms by pushing out and withdrawing the culture tank.
Description
Technical Field
The invention belongs to the technical field of deep-sea microorganisms, and particularly relates to a deep-sea microorganism in-situ culture device with a pressure compensator.
Background
The deep sea sediment is also the largest and deepest ecosystem in the world on a large scale, and contains a large amount of microorganisms. The conventional scientific research ship has the problem of discontinuity in time and space of data acquired by deep sea exploration, and the conventional sampling method based on the scientific research ship can change the components of a sample from a deep sea zone to the surface. The offshore developed countries have earlier performed the exploration and development of marine organism resources, developed a series of deep sea exploration and sampling devices, obtained a large amount of deep sea samples and experimental data. Therefore, the invention designs a deep-sea microorganism in-situ culture device for culturing and obtaining deep-sea microorganism resources.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a deep-sea microorganism in-situ culture device with a pressure compensator. The specific technical scheme is as follows:
a deep sea microorganism in-situ culture device with pressure compensation comprises a culture tank, a culture tank end cover, a culture tank shell, a connector, a motor cylinder end cover, a pressure compensation device, a watertight connector, a stepping motor, a coupler, a trapezoidal screw rod, a sealing ring, a hollow threaded rod, a plug and a fixed support, wherein the culture tank and the culture tank end cover are connected through threads; the cultivation tank and the cultivation tank end cover are movably arranged in a cultivation tank shell, the cultivation tank shell is connected with a connector through a fixing support, the connector is connected with a motor cylinder, the inner wall of a hollow threaded rod is provided with trapezoidal threads and is meshed with a trapezoidal screw rod, and the stepping motor is connected with the trapezoidal screw rod through a coupler.
Further, cultivate jar and cultivate all being equipped with the recess on the end cover and be used for installing O type circle, through O type circle realizes radial seal with the inner wall of cultivating the jar shell.
Furthermore, the connector has been seted up square through-hole, hollow threaded rod half is cylindrical before, and half section of the back is the square, and half passes the square through-hole of connector after, and the rotation of restriction hollow threaded rod realizes axial displacement. .
Furthermore, the culture tank shell, the connector and the hollow threaded rod are respectively provided with a through hole, so that the cavities of the connector, the hollow threaded rod and the culture tank shell are communicated with the outside seawater.
Further, the sealing ring sets up on the trapezoidal lead screw, the recess that inner wall and outer wall all were equipped with installation O type circle is used for realizing radial seal, and the recess of installation O type circle is seted up in connector and motor cylinder laminating department, realizes axial seal.
Furthermore, step motor passes through the motor mount pad and is fixed in the motor section of thick bamboo in, watertight connector, oil pocket formula compensator and end cap fixed mounting are on the motor section of thick bamboo end cover, and the motor section of thick bamboo end cover is installed at the open end of motor section of thick bamboo casing to realize sealed through O type circle.
Further, the one end that cultivates jar casing and keep away from cultivateing the end cover is equipped with the through-hole symmetrically, realizes exchanging with external environment through the through-hole on the cultivateing jar casing when cultivateing jar and shifting out, carries out the enrichment culture deep sea microorganism through placing solid nutrient in the cultivateing jar in advance.
Furthermore, the culture tank shell is connected with the fixed support, a bearing is arranged in the connecting body, one end of the trapezoidal screw rod is borne by the bearing, and the front half part of the hollow screw rod is connected with the end cover of the culture tank through a through hole of the culture tank shell.
The invention has the beneficial effects that:
1. the invention can perform enrichment culture and recovery of microorganisms in the deep sea environment, perform long-term observation and sampling on deep sea microorganism resources, and provide a powerful tool for further research on the deep sea microorganism resources and acquisition of gene resources.
2. The invention adopts a modular design, can be carried on deep sea equipment such as a deep sea lander, a deep sea robot and the like, and is connected with the deep sea equipment through a watertight connector. The device is convenient to be disassembled and assembled for testing in the installation and debugging stage of the device, has the characteristics of simple operation, convenient connection and the like,
3. the pressure compensator is arranged on the end cover of the motor barrel, so that the pressure inside and outside the motor barrel can be effectively balanced, the thickness of the shell of the motor barrel is reduced, and the weight of the whole set of equipment is reduced.
4. The culture tank designed by the invention can be accurately retracted into the shell of the culture tank after working underwater for a long time, and the end cover of the culture tank and the sealing ring used by the culture tank provide good sealing performance.
Drawings
FIG. 1 is a schematic view of the external structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a sectional view of a culture tank of the present invention;
FIG. 4 is a cross-sectional view of a cultured can end of the present invention;
FIG. 5 is a sectional view of a culture tank housing of the present invention;
FIG. 6 is a schematic view of the transmission of the present invention;
FIG. 7 is a front view of the connector;
fig. 8 is a sectional view of the motor cartridge.
Detailed Description
The invention will be further explained with reference to the drawings.
As shown in fig. 1 and 2, the deep-sea microorganism in-situ culture apparatus with pressure compensation according to the embodiment of the present invention includes a culture tank 1, a culture tank end cover 2, a culture tank housing 3, a connector 4, a motor barrel 5, a motor barrel end cover 6, an oil bag type pressure compensation apparatus 7, a watertight connector 8, a stepping motor 9, a coupler 10, a trapezoidal screw rod 11, a sealing ring 12, a hollow threaded rod 13, a plug 14, and a fixing bracket 15. The culture device is fixedly arranged on the deep sea lander through a fixing bracket 15.
As shown in fig. 3 and 4, the culture tank 1 is a hollow shell, a solid nutrient enrichment culture deep-sea microorganism is placed in the middle cavity, and two symmetrical through holes 101 are formed in the shell of the culture tank 1 and are used for communicating with the external environment. Grooves 102 and 202 are respectively arranged on the culture tank shell 1 and the culture tank end cover 2 and used for installing O-shaped rings so as to realize the sealing of the culture tank. The culture tank 1 is connected with the culture tank end cover 2 through screw threads.
As shown in fig. 5, the shell of the culture tank shell 3 is provided with two symmetrical through holes 301 for communicating with the external environment and preventing the equipment from being damaged due to excessive pressure, the central symmetrical through hole 401 and the through hole 303 are used for connecting the connector 4 and the fixing bracket 15 with the culture tank shell 3, the hollow threaded rod 13 passes through the through hole 302 to be connected with the culture tank end cover 2, and the culture tank end cover 2 is provided with the through hole 201 for connection.
As shown in fig. 6 and 7, the culture tank transmission device is located in the connector 4, and includes a bearing 18, a bearing 19, a sealing ring 12 and a hollow threaded rod 13 mounted on the trapezoidal screw rod 11, wherein the inner wall of the hollow threaded rod 13 has trapezoidal threads, the connector 4 is connected with the motor barrel 5 through a through hole 402 and a threaded hole 501, an O-ring is mounted in a groove 403 to realize axial sealing, and grooves are formed in the inner wall and the outer wall of the sealing ring 12 to realize radial sealing. The connecting body 4 is provided with a square through hole 408, the front half part of the hollow threaded rod 13 is cylindrical, the section of the rear half part of the hollow threaded rod is square, and the rear half part of the hollow threaded rod 13 penetrates through the square through hole 408 to limit the rotation of the hollow threaded rod 13 to realize axial movement. Through hole 405, through hole 404 and through hole 406 allow the internal space of the connector to communicate with the external environment, preventing the equipment from being damaged by the huge pressure in the deep sea.
As shown in fig. 8, the stepping motor 9 is fixed in the motor barrel 5 through the motor mounting base 16, the coupler 10 connects the stepping motor 9 and the trapezoidal lead screw 11, and the through hole 601 and the threaded hole 502 connect the motor barrel end cover 6 and the motor barrel 5. The motor barrel end cover 6 is provided with a watertight connector 8, an oil bag type pressure compensator 7 and a plug 14, the plug 14 which is hermetically connected with a threaded hole is opened when the equipment is filled with oil, air in the motor barrel 6 is released to enable the oil filling process to be simple and easy, and the stepping motor 9 is connected with control equipment on the deep sea lander through the watertight connector 8. An O-shaped ring is arranged in a groove 602 on the motor cylinder end cover 6 to realize radial sealing, and the oil sac type pressure compensator 7 is used for balancing the pressure difference between the inside and the outside of the equipment to ensure that the equipment can work normally.
The working principle of the invention is as follows:
the deep sea lander control equipment controls the stepping motor 9 to rotate forwards and backwards through the watertight connector 8, the trapezoidal screw rod 11 rotates to drive the hollow threaded rod 13 to move axially, the hollow threaded rod 13 moves axially and simultaneously drives the end cover 2 of the culture tank and the culture tank 1 to move, when the culture tank 1 moves out of the shell 3 of the culture tank, the through hole 101 in the culture tank 1 is exposed in the deep sea environment, so that seawater enters the culture tank 1 to realize microorganism enrichment culture; when equipment retrieves, cultivate jar 1 and cultivate jar end cover 2 and move into in cultivateing jar shell 3, the O type circle of recess 102 and recess 202 installation realizes sealed to it, guarantees the smooth recycle of microorganism.
Claims (7)
1. The utility model provides a from deep sea microorganism normal position culture apparatus of taking pressure compensation, includes cultivates jar (1), cultivates a jar end cover (2), cultivates a jar shell (3), connector (4), motor cylinder (5), motor cylinder end cover (6), pressure compensation device (7), watertight connector (8), step motor (9), shaft coupling (10), trapezoidal lead screw (11), sealing ring (12), hollow threaded rod (13), end cap (14) and fixed bolster (15), its characterized in that: the culture tank (1) is connected with the end cover (2) of the culture tank through threads; the culture tank (1) and the end cover (2) of the culture tank are movably arranged in a culture tank shell (3), the culture tank shell (3) is connected with a connector (4) through a fixing support (15), the connector (4) is connected with a motor cylinder (5), the inner wall of a hollow threaded rod (13) is provided with trapezoidal threads and meshed with a trapezoidal screw rod (11), and a stepping motor (9) is connected with the trapezoidal screw rod (11) through a coupler (10); square through-hole (408) have been seted up in connector (4), hollow threaded rod (13) first half is cylindrical, and the latter half cross-section is the square, and the latter half passes square through-hole (408) of connector (4), and the rotation of restriction hollow threaded rod (13) realizes axial displacement.
2. The in-situ deep-sea microorganism culture device with pressure compensation function according to claim 1, wherein grooves are formed in the culture tank (1) and the culture tank end cover (2) for installing O-rings, and the O-rings are sealed with the inner wall of the culture tank shell (3) in a radial direction.
3. The deep-sea microorganism in-situ culture device with pressure compensation function as claimed in claim 1, wherein the culture tank shell (3), the connector (4) and the hollow threaded rod (13) are respectively provided with through holes, so that the cavities of the connector (4), the hollow threaded rod (13) and the culture tank shell (3) are communicated with the outside seawater.
4. The deep sea microorganism in-situ culture device with pressure compensation function according to claim 1, wherein the sealing ring (12) is arranged on the trapezoidal screw rod (11), grooves for installing O-shaped rings are formed in the inner wall and the outer wall of the trapezoidal screw rod to realize radial sealing, and grooves (403) for installing O-shaped rings are formed in the joint of the connecting body (4) and the motor cylinder (5) to realize axial sealing.
5. The in-situ deep sea microorganism culture device with pressure compensation function according to claim 1, wherein the stepping motor (9) is fixed in the motor barrel (5) through a motor mounting seat (16), the watertight connector (8), the oil bag type compensator (7) and the plug (14) are fixedly arranged on a motor barrel end cover (6), and the motor barrel end cover (6) is arranged at the opening end of the shell of the motor barrel (5) and sealed through an O-shaped ring.
6. The in-situ deep-sea microorganism culture device with pressure compensation function according to claim 1, wherein the end of the culture tank (1) shell far away from the culture tank end cover (2) is symmetrically provided with through holes (101), when the culture tank (1) is removed, the communication with the external environment is realized through the through holes (101) on the culture tank shell, and the deep-sea microorganisms are subjected to enrichment culture by placing solid nutrients in the culture tank in advance.
7. The deep sea microbe in-situ culture device with pressure compensation function of claim 3, wherein the culture tank shell (3) is connected with a fixed bracket (15), a bearing is arranged in the connecting body (4), one end of the trapezoidal screw rod (11) is carried by the bearing, and the front half part of the hollow threaded rod (13) is connected with the culture tank end cover (2) through a through hole of the culture tank shell (3).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010440118.1A CN111471576B (en) | 2020-05-22 | 2020-05-22 | From deep sea microorganism normal position culture apparatus of taking pressure compensation |
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| CN202010440118.1A CN111471576B (en) | 2020-05-22 | 2020-05-22 | From deep sea microorganism normal position culture apparatus of taking pressure compensation |
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| CN111471576A CN111471576A (en) | 2020-07-31 |
| CN111471576B true CN111471576B (en) | 2023-03-14 |
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| CN114350507A (en) * | 2022-03-17 | 2022-04-15 | 广东工业大学 | Single colony separation device and separation method for deep sea in-situ environment |
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