CN1114701C - Fast medicine sensitivity screening method for tubercle bacillus - Google Patents
Fast medicine sensitivity screening method for tubercle bacillus Download PDFInfo
- Publication number
- CN1114701C CN1114701C CN 00131291 CN00131291A CN1114701C CN 1114701 C CN1114701 C CN 1114701C CN 00131291 CN00131291 CN 00131291 CN 00131291 A CN00131291 A CN 00131291A CN 1114701 C CN1114701 C CN 1114701C
- Authority
- CN
- China
- Prior art keywords
- tubercule bacillus
- chip
- performance
- bacillus
- tubercle bacilli
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention belongs to a fast medicine sensitivity screening method for tubercle bacilli. A uniformly mixed agent of a tested sample and various medicaments to be tested is injected onto a miniature polymer chip carved with multipath parallel channel separation grates through each injection port; the situation of the corresponding channel on the chip is monitored in one visual field of an objective lens of a microscopic picture pick-up system. The performance of the corresponding tubercle bacilli in each channel grate is analyzed by the micro picture pick-up system and a computer to obtain a movement result or a suppressed result. The method of the present invention can realize multipath synchronous analysis on the tubercle bacilli, greatly reduce the requirements for the number of tubercle bacilli and medicaments required by analysis, and greatly increase the speed of the susceptibility test of the tubercle bacilli.
Description
Technical field
The invention belongs to a kind of fast medicine sensitivity screening method to tubercule bacillus.
Technical background
Medicine sensitivity screening to tubercule bacillus in the prior art normally adopts different reagent, divide other that tubercule bacillus bacterium colony is detected test with regard to each reagent, therefrom find the medicine of the most suitable inhibition tubercule bacillus, thereby reach purpose according to different patient's symptomatic treatments.To with regard to the medicine sensitivity screening method of tubercule bacillus, need more tubercule bacillus test samples with regard to prior art, with the needs of the medicine sensitivity screening that adapts to different medicines.But, because division, the growth cycle of tubercule bacillus is longer, want usually 18 hours just division once, obtain screening and the detection that just can carry out necessity behind the tubercule bacillus of sufficient amount and test so need spend the long time that tubercule bacillus is cultivated.Until now, all methods of doing tubercule bacillus (TB) susceptibility all needed three days even reached a week or more times just can be finished both at home and abroad.This situation is very disadvantageous for the numerous crowds' that are subjected to the tubercule bacillus threat health service.
Summary of the invention
The objective of the invention is at the deficiency in the art methods, in order to solve the speed-raising purpose of tubercule bacillus drug sensitive test, proposition is on the chip of micro-polymer, tubercule bacillus is carried out parallel parsing, to shorten analytical cycle, minimizing improves the method for tubercule bacillus susceptibility speed to the needs of tubercule bacillus strain number.
Fast medicine sensitivity screening method for tubercle bacillus of the present invention and step are:
(1) to test sample through the removal of impurities bacterium pre-treatment of going out, the sample that injects in the chip only survive tubercule bacillus, other bacterium is deactivation all, and suitable enrichment;
(2) said chip is for being carved with the separate gate of multichannel channel-type arranged side by side on polymkeric substance; Have air storage bag on each passage in grid region, between air storage bag and the working section channel communication channel is arranged for the usefulness of tubercule bacillus breathing;
(3) all passages on the chip are all placed within visual field of object lens of microscope camera system, so that supply the usefulness of quick separated in synchronization analysis;
(4) with test sample and various treat an amount of mixing of reagent agent after, inject each respective channel inlet of chip through each inlet respectively;
When (5) injecting, by the situation of respective channel on the microscopic system supervision chip, behind a plurality of rod bacteriums of appearance, this passage side stops to inject in the visual field;
(6) performance of monitoring tubercule bacillus under drug effect; The performance of tubercule bacillus under drug effect, directly take in the computer through microscope camera system, the image recognition software of the performance of tubercule bacillus under drug effect can be correctly expressed in utilization, analyzes the performance of corresponding tubercule bacillus in each passage grid, and learns that it is movable or suppressed the result;
(7) draw the medicine sensitivity screening result who suppresses tubercule bacillus.
Fast medicine sensitivity screening method for tubercle bacillus of the present invention, proposition realizes on the chip of micro-polymer the separation of tubercule bacillus and the function of multichannel Synchronization Analysis, thereby reach the needs that reduce significantly tubercule bacillus bacterium quantity, a large amount of needs of analyzing required medicament that reduce, owing to adopt the mode of parallel parsing, improve analysis speed, and can process multiple signals simultaneously, in a visual field, obtain signal and transfer to Computer Analysis.Thereby increased substantially the speed of tubercule bacillus drug sensitive test.
Description of drawings
Accompanying drawing 1: the chip structure synoptic diagram that adopts in the fast medicine sensitivity screening method for tubercle bacillus.
Specific embodiments
The fast medicine sensitivity screening method of tubercule bacillus of the present invention is:
The carrier of separate gate 2 chips 1 of multichannel channel-type arranged side by side as check sample is carved with in utilization on polymkeric substance; All have air storage bag 3 on each passage in said separate gate grid region, between air storage bag and the working section channel communication channel is arranged for the usefulness of tubercule bacillus breathing.
The working zone (the straight-through district of band air storage bag as shown in Figure 1) of all passages on the chip 1 is all placed within visual field 4 of object lens of microscope camera system, so that supply the usefulness of quick separated in synchronization analysis.
Test sample 5 (tubercule bacillus) the removal of impurities bacterium that should do to go out before check is handled, and the sample that injects in the chip only survive tubercule bacillus, and suitable enrichment; With test sample with various different treat an amount of mixing of reagent agent after, each is added with the medicament of check sample respectively among each inlet 6 is injected into chip 1 each respective channel; When injecting, monitor the situation of respective channel on the chip by microscopic system, a plurality of rod bacteriums that active performance arranged appear until visual field 4 in and after, this passage stops injection.
After the sample injection, through the performance of microscope camera system monitoring tubercule bacillus under drug effect; With the performance of tubercule bacillus under drug effect, directly take in the computer through microscope camera system, the image recognition software of the special use of the performance of tubercule bacillus under drug effect can be correctly expressed in utilization, analyze the situation of corresponding tubercule bacillus in each passage grid, thereby learn the active situation of its tubercule bacillus or suppressed the result; Finally draw the medicine sensitivity screening result who suppresses tubercule bacillus.
Said microscope camera system preferably has 10bit or the above quantified precision of 10bit, to guarantee knowing that the faint changing condition of target, its work light-wave band are 0.4～0.95 μ m;
The lighting source of said chip 1 can be the below, or top, or dual inclination executes photograph, and used light source can be the halogen tungsten lamp light source that fibre bundle is introduced.
Claims (1)
1, a kind of fast medicine sensitivity screening method for tubercle bacillus is:
(1) to test sample through the removal of impurities bacterium pre-treatment of going out, the sample that injects in the chip is only survived tubercule bacillus, other bacterium is deactivation all;
(2) said chip is for being carved with the separate gate of multichannel channel-type arranged side by side on polymkeric substance; Have air storage bag on each passage in grid region for the usefulness of tubercule bacillus breathing;
(3) working zone with all passages on the chip all places within visual field of object lens of microscope camera system;
(4) with test sample and various treat an amount of mixing of reagent agent after, inject each respective channel inlet of chip through each inlet respectively;
When (5) injecting, monitor the situation of respective channel on the chip by microscopic system, a plurality of rod bacteriums that active performance arranged appear in the visual field and after, this passage side stops to inject;
(6) performance of monitoring tubercule bacillus under drug effect; The performance of tubercule bacillus under drug effect, directly take in the computer through microscope camera system, the image recognition software of the performance of tubercule bacillus under drug effect can be correctly expressed in utilization, analyzes the performance of corresponding tubercule bacillus in each passage grid, and learns that it is movable or suppressed the result.
(7) draw the medicine sensitivity screening result who suppresses tubercule bacillus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00131291 CN1114701C (en) | 2000-12-20 | 2000-12-20 | Fast medicine sensitivity screening method for tubercle bacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00131291 CN1114701C (en) | 2000-12-20 | 2000-12-20 | Fast medicine sensitivity screening method for tubercle bacillus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1299878A CN1299878A (en) | 2001-06-20 |
| CN1114701C true CN1114701C (en) | 2003-07-16 |
Family
ID=4594609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00131291 Expired - Fee Related CN1114701C (en) | 2000-12-20 | 2000-12-20 | Fast medicine sensitivity screening method for tubercle bacillus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1114701C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5828177B2 (en) | 2011-09-13 | 2015-12-02 | 国立大学法人大阪大学 | Bacterial or fungal antimicrobial susceptibility testing method and system used therefor |
-
2000
- 2000-12-20 CN CN 00131291 patent/CN1114701C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1299878A (en) | 2001-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Geme III et al. | Distinguishing sepsis from blood culture contamination in young infants with blood cultures growing coagulase-negative staphylococci | |
| Tyndall et al. | Application of flow cytometry to detection and characterization of Legionella spp | |
| Holmberg et al. | Identification of Actinomyces, Arachnia, Bacterionema, Rothia, and Propionibacterium species by defined immunofluorescence | |
| CN107287107A (en) | A kind of circulating tumor cell separation equipment, system and method | |
| CN110824032B (en) | Fingerprint identification method for bombyx batryticatus and counterfeit bombyx batryticatus | |
| CN102288755B (en) | PDMS (Polydimethylsiloxane) multichannel immunoassay chip for rapid field detection of microorganisms | |
| CN109266717A (en) | A kind of method and apparatus by single cell analysis detection bacterium drug resistance | |
| EP2640843A1 (en) | Viability cell counting by differential light absorption | |
| CN1114701C (en) | Fast medicine sensitivity screening method for tubercle bacillus | |
| CN115029244A (en) | Micro-fluidic chip for sorting circulating tumor cells and analyzing single-cell horizontal multi-dimensional heterogeneity and application | |
| AU2018235992B2 (en) | Device, method, and system for identifying organisms and determining their sensitivity to toxic substances using the changes in the concentrations of metabolites present in growth medium | |
| CN114317400A (en) | Exosome separation purification detection method and device | |
| DE69731982T2 (en) | METHOD FOR MEASURING THE LYMPHOCYTE FUNCTION | |
| CN1458527A (en) | Immune chromatographic paper strip and method for quick detecting pathogen and toxin in food | |
| CN105838667B (en) | A kind of TGF-β signal path function verification method in the Huang chicken male sex-cell atomization of Rugao | |
| CN1390949A (en) | Reagent kit for detecting different microbes with colloidal gold by one step | |
| CN106950163B (en) | Method for detecting chicken thymus T lymphocyte subpopulation by flow cytometry | |
| Shields et al. | Invasion of collagen gels by mouse lymphoid cells. | |
| CN101570739B (en) | Method for building psoriasis basic research models, and gas-liquid level transmembrane device | |
| CN102768283B (en) | Kit for detecting or supplementarily detecting medullary leukemia cell differentiation stages | |
| US20230249182A1 (en) | Microfluidic chip device based on magnetic field-controlled fluorescently-labeled cell sorting method and use method | |
| CN114015741B (en) | Non-invasive cell activity analysis method | |
| CN1114700C (en) | Chip for the separation, analysis and treatment of bacteria and cells | |
| CN113567407A (en) | Method for detecting mitochondrial function of hematopoietic cells | |
| Zinner et al. | Use of fluorescent-antibody technique to identify experimental hamster and rat strains of cariogenic streptococci |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |