CN111454973A - Eggplant fruit color epistatic P gene locus candidate gene and application method thereof - Google Patents
Eggplant fruit color epistatic P gene locus candidate gene and application method thereof Download PDFInfo
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Abstract
The invention discloses a candidate gene of an eggplant fruit color epistatic P gene locus; also discloses an eggplant Sm ANS-1 mutant gene, wherein the Sm ANS-1 mutant gene comprises 1 nonsense mutation compared with known eggplant Sm ANS gene sequences (EU809469 and KT727966), so that translation is terminated early, and the function of anthocyanin synthetase is lost. The invention also discloses the function of the candidate gene of the upper P locus of the eggplant fruit color in the upper inheritance of the eggplant fruit color and the application of the candidate gene in the improvement of the fruit color.
Description
Technical Field
The invention belongs to the field of molecular biology, particularly relates to the technical field of plant genetic engineering, and particularly relates to positioning cloning, mutant gene analysis and application of candidate genes related to synthesis of eggplant anthocyanin.
Background
Eggplant fruit color inheritance phenomenon controlled by 2 pairs of epistatic effector genes is found in eggplant breeding practice of vegetable research of Guangdong provincial academy of agricultural sciences. I.e. after crossing 2 homozygous white eggplant plants, F1The fruit substitute color is purple red (figure 1), F2The segregation ratio of the generation rhododendron and white fruit plants is 9:7, which shows that the peel coloring is controlled by 2 genetic loci with epistasis, 2 gingko color eggplant is caused by the inactivation of 2 independent and complementary genetic loci (D gene and P gene) for controlling anthocyanin biosynthesis process, the gingko color male parent and female parent genotypes are ddPP and DDPP respectively, the genetic loci for controlling the color epistasis of eggplant are defined as D gene and P gene respectively in the existing references (tomato longitudinal L.). Genetics,1968,60: 475-.
At present, specific genes of the eggplant fruit color epistasis gene locus are not clear, so that the research is based on the research of locating the eggplant fruit color epistasis P gene (Z L201810018063.8) in the previous period, and the candidate genes are dug by performing gene function annotation in a close association region on the epistasis P gene of the eggplant fruit color located on an 8 chromosome, and SmANS is considered as the P gene candidate gene.
A large number of experiments prove that the ANS gene plays a key role in an anthocyanin synthesis pathway and has certain specificity in different tissues and development stages of plants. ANS plays an important role in the synthesis of anthocyanins, and mutations in its key structure can result in the failure to accumulate anthocyanins. The research clones male parent SmANS genes and female parent SmANS genes to obtain full-length mRNA and DNA sequences, and finds that the female parent SmANS genes generate nonsense mutation by comparing sequence difference between the male parent and the female parent; the function of SmANS genes in the color epistasis inheritance of eggplant fruits is researched by constructing SmANS gene overexpression vectors and converting ginkgo color master mutants. The research can verify the reliability of the early positioning result, can also define the action mechanism of the candidate gene SmANS for regulating and controlling anthocyanin synthesis under the genetic background of the upper position of eggplant fruit color, and has important theoretical and guiding significance for eggplant fruit color molecular breeding in future.
Disclosure of Invention
The invention aims to screen candidate genes of a P gene locus for controlling the color epistasis inheritance of eggplants, to determine the mutation types of the candidate genes and to regulate the action mechanism of the synthesis of the anthocyanin of the eggplant peel in the color epistasis genes of the eggplants.
The technical scheme adopted by the invention is as follows:
predicting and annotating genes in the fine positioning associated region, and screening genes related to anthocyanin metabolic pathways; amplifying P gene locus candidate genes by respectively taking a female parent (genotype DDpp) and a ginkgo color male parent (genotype ddPP) as templates to determine female parent (pp) mutant gene information; extracting male parent RNA as a template, amplifying SmANS gene by RT-PCR according to sequence information of a self-owned transcriptome database, and constructing a 35S promoter overexpression vector pBWA (V) HS-Sm ANS; the mutant maternal cotyledon and hypocotyl are transformed by adopting an agrobacterium-mediated leaf disc method, and the synthesis expression of the callus and the transformed adventitious bud anthocyanin is observed in the transformation culture process.
Specifically, the candidate gene of the eggplant fruit color epistatic P gene locus is anthocyanin synthase (ANS) Sm ANS.
And obtaining a mother eggplant Sm ANS-1 mutant gene sequence based on the candidate gene, wherein the cDNA sequence is shown as SEQ ID NO.1, and the DNA sequence is shown as SEQ ID NO. 2.
Compared with the known eggplant Sm ANS gene sequence, the Sm ANS-1 mutant gene comprises 1 nonsense mutation, namely, the base T at the position of +101 is mutated into A, and the triplet codon TTA is mutated into TAA, so that translation is terminated early, and the function of anthocyanin synthetase is lost.
An application method of candidate genes of eggplant fruit color epistatic P gene loci constructs a pBWA (V) HS-Sm ANS overexpression vector, Sm ANS-1 mutants are transformed by an agrobacterium-mediated method, and results show that mauve is observed on the back sides of transformed mutant callus and adventitious bud leaf stalks, which indicates that the anthocyanin synthesis of ginkgo color masterbatch mutant anthocyanin is recovered by overexpression of the Sm ANS genes.
The invention has the beneficial effects that: on the basis of finely positioning the upper P gene locus of the eggplant fruit color, genes in a finely positioned related region are predicted and annotated, and then genes related to anthocyanin metabolic pathways are screened through bioinformatics analysis such as GO enrichment and KEGG enrichment, so that an upper P gene candidate gene of the eggplant fruit color can be obtained; gene mutation can be determined by gene amplification analysis and comparison of candidate genes in different genotype male parent and female parent materials; the normal wild type gene of the male parent is overexpressed in the mutant female parent material, and the anthocyanin synthesis condition of the transformed tissue is observed, so that the accuracy of the positioning result can be verified, the function of the candidate gene in the regulation and control of the color position of the eggplant can be determined, and an important theoretical basis and technical means can be provided for the molecular breeding of the color of the eggplant.
Drawings
FIG. 1: the fruit color of the parent material Female is white, and no anthocyanin is observed in hypocotyl, vein, flower and sepal during the whole growth period; male parent Male is white in fruit color, hypocotyl is purplish slightly in seedling stage, leaf vein is purplish slightly, flower color is purplish slightly, F1The hypocotyls, veins, flower color and fruit color of the substitute materials are all purple red (a, b); c is a moiety F2And (5) separating the fruit color of the single plant.
FIG. 2: the parent (Female) SmANS-1cDNA sequence was aligned with the parent (Male) SmANS cDNA, known SmANS sequences (EU809469 and KT727966) and the reference genome annotated gene (Sme2.5_01638.1_ g 00005.1).
FIG. 3 shows the cDNA sequence of the parent SmANS and its predicted amino acid sequence, the terminator is indicated by a dotted letter, the mutation site is indicated by a red box, the conserved domain P L N03176 sequence is indicated by a black underline, the conserved domain DIOX-N sequence is indicated by a black box, and the conserved domain 2 OG-FeII-Oxy sequence is indicated by a red underline.
FIG. 4 shows construction of pBWA (V) HS-Sm ANS eggplant over-expression vector, A is SmANS gene amplification electrophoresis detection result, M1 is Marker [ D L6000 ], (6000, 4000, 3000, 2000, 1500, 1000, 750, 500, 250 and 100bp from top to bottom), 1 is male parent Sm ANS gene (1242bp), B is EcorV enzyme digestion electrophoresis detection result pBWA (V) HS-Sm ANS plasmid, M2 is Marker [ D L5000 ], (5000, 3000, 2000, 1000, 750, 500, 250 and 100bp, 2 is EcorV enzyme digestion band 1134, 1843, 2691 and 2624 bp, and C is pBWA (V) HS-Sm vector structure diagram.
FIG. 5: influence of overexpression of SmANS gene on anthocyanin synthesis of white female parent mutant. A is negative control; b, synthesizing anthocyanin from the callus; c is synthesized anthocyanin on the back of the leaf stalk of the transformed adventitious bud.
Detailed Description
The present invention will be further described with reference to the accompanying drawings, and it should be noted that the following examples are provided to illustrate the detailed embodiments and specific operations based on the technical solutions of the present invention, but the scope of the present invention is not limited to the examples.
Example 1
Screening of candidate genes of P sites on fruit color epistasis of eggplants
1. Test materials
2 parts of white eggplant purified inbred line Male parent (Male, genotype is ddPP) and Female parent (Female, genotype is DDPP) which are bred by strictly bagging and selfing at the vegetable research institute of Guangdong province academy of agricultural sciences for multiple generations, and a hybrid combination 3316F1 prepared by the Male parent and the Female parent are plant materials. The fruit color of the parent material Female is white, and no anthocyanin is observed in hypocotyl, vein, flower and sepal during the whole growth period; male parent Male is white in fruit color, hypocotyl is purplish slightly in seedling stage, leaf vein is purplish slightly, flower color is purplish slightly, F1The hypocotyls, veins, flower color and fruit color of the generation material all appeared purple red (fig. 1a, b). F2The segregation ratio of the segregating generations of purple red and white fruit plants (fig. 1c) was checked by chi square to be 9: 7.
2. Screening result of candidate genes of P locus on fruit color of eggplant
According to the positioning result of the QT L gene of the eggplant fruit color and the distribution of Marker on chromosome, searching genes in the character candidate region, and performing gene function annotation, the statistics are shown in Table 1, and the gene Sme2.5_01638.1_ g00005.1 is annotated on Scarford Sme2.5_01638.1 in the positioning region, and is Sm ANS gene.
TABLE 1 QT L result location Gene annotation statistics
Amplification and comparative analysis of Sm ANS gene in male parent and female parent
RNA extraction and reverse transcription
Total RNA of the samples was extracted using a TransZol Plant kit (Beijing Quanyujin Biotechnology Co., Ltd.), and 1. mu.g of the total RNA of each sample was used as a template with PrimeScriptTMRT reagent kit with gDNA Eraser (Perfect Real Time) kit (Takara, Boehringer Bio Inc.) removes genomic DNA and reverse transcribes to synthesize the first strand of cDNA, which is stored at-20 ℃ for use as a template for PCR amplification.
2. Primer design
Specific primers are designed in different sequence sections according to Unigene0020595 (encoding eggplant anthocyanin synthase SmANS, self transcriptome sequence, unpublished) related to synthesis of eggplant pericarp anthocyanin and reference genome sequence information, and amplification, splicing and verification are carried out on Sm ANS gene cDNA sequence and DNA. The primer sequences and target fragment sizes are detailed in Table 2.
TABLE 2 primer sequences and sizes
The result of the alignment of the Sm ANS gene cDNA sequence and DNA sequence
The result of the splicing of the Sm ANS gene cDNA sequence and the DNA sequence is shown as SEQ ID NO.1 and SEQ ID NO.2 respectively. The sequence alignment results are shown in fig. 2, the parent (Female) SmANS-1cDNA sequence has 1T/A base difference compared with the parent (Male) SmANS cDNA, the known SmANS sequences (EU809469 and KT727966) and the reference genome annotation gene (Sme2.5_01638.1_ g00005.1), and the rest is T except the parent is A. Further analysis showed that the mutation of base T at position +101 of SmANS-1cDNA sequence to A, the mutation of triplet codon TTA to TAA (stop codon) and nonsense mutation (FIG. 3) resulted in premature termination of translation and loss of anthocyanin synthase function.
Example 2
Effect of eggplant fruit overexpression SmANS gene on anthocyanin synthesis of maternal mutant
pBWA (V) HS-Sm ANS eggplant over-expression vector construction.
PCR amplification reaction is carried out by taking cDNA of male parent eggplant as a template to obtain SmANS gene over-expression CDS sequence (figure 4A), the obtained SmANS gene fragment is connected with pBWA (V) HS vector, and the enzyme cutting electrophoresis detection result of figure 4B shows that pBWA (V) HS SmANS is cut by enzyme to obtain 1372bp target fragment, which indicates that pBWA (V) HS-SmANS recombinant vector is successfully constructed (figure 4C).
2. Effect of eggplant fruit overexpression SmANS gene on anthocyanin synthesis of different tissue parts of female parent mutant
The SmANS gene of the female parent is mutated into SmANS-1, so that various tissue parts (including stems, flowers, fruits and the like) of the female parent cannot synthesize anthocyanin, the whole body of a nutritive tissue is green, the flowers are white, and the fruit peel is also white. Anthocyanin synthesis was observed on maternal mutant calli overexpressing the SmANS gene (FIG. 5B) and a purple-red color was also observed on the back of the petiole of the transformed regenerated adventitious bud, compared to the negative control (FIG. 5A). The result proves that the SmANS gene is a candidate gene of the upper P gene locus of the eggplant fruit color, the P locus SmANS-1 gene mutant is a root cause that the female parent can not normally synthesize anthocyanin, and the SmANS is one of upper regulation genes of the eggplant fruit color and plays an important role in the upper genetic inheritance of the eggplant fruit color.
Various corresponding changes and modifications can be made by those skilled in the art based on the above technical solutions and concepts, and all such changes and modifications should be included in the protection scope of the present invention.
Sequence listing
<110> vegetable research institute of academy of agricultural sciences of Guangdong province
<120> eggplant fruit color epistatic P gene locus candidate gene and application method thereof
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>3
<212>DNA
<213>2 Ambystoma laterale x Ambystoma jeffersonianum
<400>1
Claims (4)
1. A candidate gene of an eggplant fruit color epistatic P gene locus is characterized in that the candidate gene is an anthocyanin synthase (ANS) Sm ANS.
2. The candidate gene of the eggplant fruit color epistatic P gene locus as claimed in claim 1, characterized in that a mother eggplant Sm ANS-1 mutant gene sequence is obtained based on the mutation of the candidate gene, wherein the cDNA sequence is shown as SEQ ID No.1 and the DNA sequence is shown as SEQ ID No. 2.
3. The candidate gene of eggplant fruit color epistatic P gene locus as claimed in claim 3, characterized in that the SmANS-1 mutant gene comprises a nonsense mutation at 1, i.e. base T is mutated to A at position +101 and triplet TTA is mutated to TAA at position +101, compared to the known Sm ANS gene sequence, resulting in premature termination of translation and loss of anthocyanin synthase function.
4. The application method of the candidate gene of the eggplant fruit color epistatic P gene locus as claimed in claim 1, characterized in that a pBWA (V) HS-Sm ANS overexpression vector is constructed, and Sm ANS-1 mutant is transformed by an agrobacterium-mediated transformation method, and as a result, purple red is observed on the back of transformed mutant callus and adventitious bud leaf stalk, which indicates that the gingko color masterbatch mutant anthocyanin synthesis is recovered by overexpression of the Sm ANS gene.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111910013A (en) * | 2020-08-20 | 2020-11-10 | 广东省农业科学院蔬菜研究所 | InDel molecular marker closely linked with anthocyanin synthetic gene of eggplant, primer and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701926A (en) * | 2016-12-09 | 2017-05-24 | 广东省农业科学院蔬菜研究所 | Mapping of eggplant fruit color epistatic gene D, and InDel molecular marker development and application thereof |
| CN108034752A (en) * | 2018-01-09 | 2018-05-15 | 广东省农业科学院蔬菜研究所 | InDel molecular labelings and application with eggplant fruit color epistatic gene P close linkages |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701926A (en) * | 2016-12-09 | 2017-05-24 | 广东省农业科学院蔬菜研究所 | Mapping of eggplant fruit color epistatic gene D, and InDel molecular marker development and application thereof |
| CN108034752A (en) * | 2018-01-09 | 2018-05-15 | 广东省农业科学院蔬菜研究所 | InDel molecular labelings and application with eggplant fruit color epistatic gene P close linkages |
Non-Patent Citations (5)
| Title |
|---|
| DOCIMO,T.等: "ACCESSION:KT727966.1", 《GENBANK》 * |
| MENGQIANG CHEN等: "Fine Mapping Identifies SmFAS Encoding an Anthocyanidin Synthase as a Putative Candidate Gene for Flower Purple Color in Solanum melongena L.", 《INT J MOL SCI.》 * |
| W. S. DE JONG等: "Candidate gene analysis of anthocyanin pigmentation loci in the Solanaceae", 《THEOR APPL GENET》 * |
| 孙保娟等: "茄子果色上位P基因紧密连锁的InDel分子标记及其应用", 《中国园艺学会2019年学术年会暨成立90周年纪念大会》 * |
| 廖毅等: "与茄子果皮颜色相关联的AFLP 及SCAR 标记", 《中国农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111910013A (en) * | 2020-08-20 | 2020-11-10 | 广东省农业科学院蔬菜研究所 | InDel molecular marker closely linked with anthocyanin synthetic gene of eggplant, primer and application thereof |
| CN111910013B (en) * | 2020-08-20 | 2021-11-19 | 广东省农业科学院蔬菜研究所 | InDel molecular marker closely linked with anthocyanin synthetic gene of eggplant, primer and application thereof |
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