CN111450052A - Preparation method of urokinase injection - Google Patents
Preparation method of urokinase injection Download PDFInfo
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- CN111450052A CN111450052A CN202010320749.XA CN202010320749A CN111450052A CN 111450052 A CN111450052 A CN 111450052A CN 202010320749 A CN202010320749 A CN 202010320749A CN 111450052 A CN111450052 A CN 111450052A
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- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
- A61K38/49—Urokinase; Tissue plasminogen activator
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- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
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- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
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Abstract
The invention discloses a preparation method of a urokinase injection, relating to the technical field of urokinase preparation, wherein the preparation method of the urokinase injection comprises the following steps: preparing crude urokinase: (1) adsorption, (2) elution, (3) crude product preparation; (II) preparing a urokinase fine product: (1) dissolving, (2) preparing a fine product, (3) purifying; (III) preparing a urokinase injection: (1) examination, (2) measurement, (3) injection preparation. According to the preparation method of the urokinase injection, the crude urokinase prepared by the process (I) can be extracted from urine, and the preparation method is simple and is convenient for industrial mass production; the fine urokinase product prepared by the step (II) can be used for separating and purifying a crude urokinase product, so that the urokinase with less impurities and higher purity can be obtained, the stability is good, and the problems of poor product quality and low yield can be solved conveniently.
Description
Technical Field
The invention relates to the technical field of urokinase, in particular to a preparation method of urokinase injection.
Background
Urokinase is an enzyme protein isolated from healthy human urine, or obtained from human kidney tissue culture. The urokinase can be directly acted on an endogenous fibrinolysis system, can catalyze and crack plasminogen plasmin and plasmin, and the plasmin can degrade fibrin clots and can also degrade fibrinogen, blood coagulation factor V, blood coagulation factor VIII and the like in blood circulation, thereby exerting the thrombolysis effect. Urokinase can also improve the activity of vascular ADP enzyme, inhibit platelet aggregation induced by ADP, and prevent thrombosis. Therefore, the method has wide application in medical drugs.
At present, urokinase finished products prepared from urokinase injection have more impurities, the purity of urokinase extracted during preparation is insufficient, the urokinase injection is not checked and measured during preparation, and the extraction quality is poor, so that a preparation method of the urokinase injection is provided.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation method of urokinase injection, which solves the problems that the prior urokinase injection prepared in the background art has more impurities, the purity of urokinase extracted during preparation is insufficient, the urokinase injection is not checked and determined during preparation, and the extraction quality is poor
In order to achieve the purpose, the invention is realized by the following technical scheme: a preparation method of a urokinase injection comprises the following steps:
preparing a crude urokinase product;
secondly, preparing a fine urokinase product;
and (III) preparing urokinase injection.
Optionally, the step (one) in the preparation of the urokinase injection comprises the following specific steps:
preparing crude urokinase:
(1) and adsorption: preliminarily filtering qualified urine, then filtering the urine into a mixing tank, adding D-160A ion exchange resin to adsorb the urine, and simultaneously uniformly and slowly mixing and stirring the urine in the mixing tank to ensure that the adsorption is more sufficient;
(2) and (3) elution: washing D-160A ion exchange resin with clear water to remove impurities, eluting with ammonia water, uniformly stirring and mixing the ammonia water in the elution process, and collecting the eluent;
(3) and preparing a crude product: adding solid ammonium sulfate into the eluent, stirring and dissolving the solid ammonium sulfate, fully precipitating again, and finally filtering the precipitation solution to obtain filter residue, namely a crude urokinase product.
Optionally, the second step in the preparation of the urokinase injection comprises the following specific steps:
(II) preparing a urokinase fine product:
(1) and dissolving: dissolving the crude urokinase by using a phosphate buffer solution of ph6.0, uniformly mixing and stirring the urokinase in the dissolving process, and accelerating the dissolving process;
(2) and fine product preparation: after dissolving, filtering the solution, then precipitating again, dehydrating and drying to obtain a fine urokinase product;
(3) and purifying: dissolving the fine urokinase product in water to obtain a solution, performing centrifugal filtration to obtain a clear solution, adjusting the conductivity by using a balance buffer solution, performing elution by using an elution buffer solution, collecting the elution solution, filtering the elution solution, and performing freeze drying to obtain the high-purity fine urokinase product.
Optionally, the step (iii) in the preparation of the urokinase injection comprises the following specific steps:
(III) preparing a urokinase injection:
(1) and checking: taking high-purity fine urokinase, adding 0.9% sodium chloride solution to prepare solution containing 3000 units per 1ml, and checking the color of the solution;
(2) and (3) measuring: dissolving high-purity urokinase fine product in water to prepare a solution containing 2mg of urokinase per 1ml, adding an equal volume of buffer solution, placing the buffer solution in a water bath for cooling, and measuring by using an electrophoresis method;
(3) and preparing an injection: after checking and determining, taking high-purity fine urokinase, adding water for injection, stirring uniformly and adjusting concentration to obtain clear and colorless injection, and obtaining the urokinase injection with qualified and stable quality.
Optionally, the concentration of ammonia water eluted in the step (one) is 3%, and the average stirring time is 2 hours.
Optionally, the centrifugal rotation speed in the centrifugal process in the step (II) is 3500rpm, and the processing temperature is lower than 15 ℃.
Optionally, the determination buffer in the step (three) is prepared by mixing a concentrated gel buffer, a sodium dodecyl sulfate solution, a bromophenol blue solution, a glycerol solution and water.
Optionally, the water for injection in the step (three) may be prepared by using sterilized normal saline or 5% glucose solution.
The preparation method of the urokinase injection has the following advantages:
the crude urokinase prepared by the process (I) can be extracted from urine, and the preparation is simple and is convenient for industrial mass production; the fine urokinase product prepared by the step (II) can be used for separating and purifying a crude urokinase product, so that the urokinase with less impurities and higher purity can be obtained, the stability is good, and the problems of poor product quality and low yield can be solved conveniently; through the checking and measuring steps of preparing the urokinase injection in the step (III), the quality of the extracted urokinase can be checked, the production quality of products is guaranteed, and the fine urokinase can be dissolved by preparing injection water by adopting sterilized normal saline or 5% glucose solution, so that the urokinase injection can be obtained conveniently.
Detailed Description
A preparation method of urokinase injection comprises the following steps:
preparing a crude urokinase product;
secondly, preparing a fine urokinase product;
and (III) preparing urokinase injection.
The preparation process of the urokinase injection (I) comprises the following specific steps:
preparing crude urokinase:
(1) and adsorption: preliminarily filtering qualified urine, then filtering the urine into a mixing tank, adding D-160A ion exchange resin to adsorb the urine, and simultaneously uniformly and slowly mixing and stirring the urine in the mixing tank to ensure that the adsorption is more sufficient;
(2) and (3) elution: washing D-160A ion exchange resin with clear water to remove impurities, eluting with ammonia water, uniformly stirring and mixing the ammonia water in the elution process, and collecting the eluent;
(3) and preparing a crude product: adding solid ammonium sulfate into the eluent, stirring and dissolving the solid ammonium sulfate, fully precipitating again, and finally filtering the precipitation solution to obtain filter residue, namely a crude urokinase product;
the concentration of ammonia eluted in the step (I) was 3%, and the average stirring time was 2 hours.
The second procedure in the preparation of the urokinase injection comprises the following specific steps:
(II) preparing a urokinase fine product:
(1) and dissolving: dissolving the crude urokinase by using a phosphate buffer solution of ph6.0, uniformly mixing and stirring the urokinase in the dissolving process, and accelerating the dissolving process;
(2) and fine product preparation: after dissolving, filtering the solution, then precipitating again, dehydrating and drying to obtain a fine urokinase product;
(3) and purifying: dissolving the fine urokinase product in water to obtain a solution, performing centrifugal filtration to obtain a clear solution, adjusting the conductivity by using a balance buffer solution, performing elution by using an elution buffer solution, collecting the elution solution, filtering the elution solution, and performing freeze drying to obtain a high-purity fine urokinase product;
the centrifugal speed in the centrifugal process in the working procedure (II) is 3500rpm, and the processing temperature is lower than 15 ℃.
The third step in the preparation of the urokinase injection comprises the following specific steps:
(III) preparing a urokinase injection:
(1) and checking: taking high-purity fine urokinase, adding 0.9% sodium chloride solution to prepare solution containing 3000 units per 1ml, and checking the color of the solution;
(2) and (3) measuring: dissolving high-purity urokinase fine product in water to prepare a solution containing 2mg of urokinase per 1ml, adding an equal volume of buffer solution, placing the buffer solution in a water bath for cooling, and measuring by using an electrophoresis method;
(3) and preparing an injection: after checking and determining, taking high-purity fine urokinase, adding water for injection, stirring uniformly and adjusting concentration to obtain clear and colorless injection, and obtaining the urokinase injection with qualified and stable quality.
The determination buffer solution in the third step is prepared by mixing concentrated gel buffer solution, sodium dodecyl sulfate solution, bromophenol blue solution, glycerol solution and water, and the water for injection in the third step can be prepared by sterilized normal saline or 5% glucose solution.
In summary, the preparation method of the urokinase injection comprises the following steps:
preparing crude urokinase:
(1) and adsorption: preliminarily filtering qualified urine, then filtering the urine into a mixing tank, adding D-160A ion exchange resin to adsorb the urine, and simultaneously uniformly and slowly mixing and stirring the urine in the mixing tank to ensure that the adsorption is more sufficient;
(2) and (3) elution: washing D-160A ion exchange resin with clear water to remove impurities, eluting with ammonia water, uniformly stirring and mixing the ammonia water in the elution process, and collecting the eluent;
(3) and preparing a crude product: adding solid ammonium sulfate into the eluent, stirring and dissolving the solid ammonium sulfate, fully precipitating again, and finally filtering the precipitation solution to obtain filter residue, namely a crude urokinase product.
(II) preparing a urokinase fine product:
(1) and dissolving: dissolving the crude urokinase by using a phosphate buffer solution of ph6.0, uniformly mixing and stirring the urokinase in the dissolving process, and accelerating the dissolving process;
(2) and fine product preparation: after dissolving, filtering the solution, then precipitating again, dehydrating and drying to obtain a fine urokinase product;
(3) and purifying: dissolving the fine urokinase product in water to obtain a solution, performing centrifugal filtration to obtain a clear solution, adjusting the conductivity by using a balance buffer solution, performing elution by using an elution buffer solution, collecting the elution solution, filtering the elution solution, and performing freeze drying to obtain the high-purity fine urokinase product.
(III) preparing a urokinase injection:
(1) and checking: taking high-purity fine urokinase, adding 0.9% sodium chloride solution to prepare solution containing 3000 units per 1ml, and checking the color of the solution;
(2) and (3) measuring: dissolving high-purity urokinase fine product in water to prepare a solution containing 2mg of urokinase per 1ml, adding an equal volume of buffer solution, placing the buffer solution in a water bath for cooling, and measuring by using an electrophoresis method;
(3) and preparing an injection: after checking and determining, taking high-purity fine urokinase, adding water for injection, stirring uniformly and adjusting concentration to obtain clear and colorless injection, and obtaining the urokinase injection with qualified and stable quality.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A method for preparing a urokinase injection is characterized by comprising the following steps:
preparing a crude urokinase product;
secondly, preparing a fine urokinase product;
and (III) preparing urokinase injection.
2. The preparation method of the urokinase injection is characterized in that the preparation process (I) of the urokinase injection comprises the following specific steps:
preparing crude urokinase:
(1) and adsorption: preliminarily filtering qualified urine, then filtering the urine into a mixing tank, adding D-160A ion exchange resin to adsorb the urine, and simultaneously uniformly and slowly mixing and stirring the urine in the mixing tank to ensure that the adsorption is more sufficient;
(2) and (3) elution: washing D-160A ion exchange resin with clear water to remove impurities, eluting with ammonia water, uniformly stirring and mixing the ammonia water in the elution process, and collecting the eluent;
(3) and preparing a crude product: adding solid ammonium sulfate into the eluent, stirring and dissolving the solid ammonium sulfate, fully precipitating again, and finally filtering the precipitation solution to obtain filter residue, namely a crude urokinase product.
3. The preparation method of the urokinase injection is characterized in that the second step in the preparation of the urokinase injection comprises the following specific steps:
(II) preparing a urokinase fine product:
(1) and dissolving: dissolving the crude urokinase by using a phosphate buffer solution of ph6.0, uniformly mixing and stirring the urokinase in the dissolving process, and accelerating the dissolving process;
(2) and fine product preparation: after dissolving, filtering the solution, then precipitating again, dehydrating and drying to obtain a fine urokinase product;
(3) and purifying: dissolving the fine urokinase product in water to obtain a solution, performing centrifugal filtration to obtain a clear solution, adjusting the conductivity by using a balance buffer solution, performing elution by using an elution buffer solution, collecting the elution solution, filtering the elution solution, and performing freeze drying to obtain the high-purity fine urokinase product.
4. The preparation method of the urokinase injection is characterized in that the working procedure (III) in the preparation of the urokinase injection comprises the following specific steps:
(III) preparing a urokinase injection:
(1) and checking: taking high-purity fine urokinase, adding 0.9% sodium chloride solution to prepare solution containing 3000 units per 1ml, and checking the color of the solution;
(2) and (3) measuring: dissolving high-purity urokinase fine product in water to prepare a solution containing 2mg of urokinase per 1ml, adding an equal volume of buffer solution, placing the buffer solution in a water bath for cooling, and measuring by using an electrophoresis method;
(3) and preparing an injection: after checking and determining, taking high-purity fine urokinase, adding water for injection, stirring uniformly and adjusting concentration to obtain clear and colorless injection, and obtaining the urokinase injection with qualified and stable quality.
5. The lead filtering method of a ceramic material manufacturing apparatus as claimed in claim 1, wherein: the concentration of ammonia water eluted in the step (one) was 3%, and the average stirring time was 2 hours.
6. The lead filtering method of a ceramic material manufacturing apparatus as claimed in claim 1, wherein: the centrifugal rotating speed in the centrifugal process in the working procedure (II) is 3500rpm, and the processing temperature is lower than 15 ℃.
7. The lead filtering method of a ceramic material manufacturing apparatus as claimed in claim 1, wherein: the determination buffer solution in the step (III) is prepared by mixing a concentrated gel buffer solution, a sodium dodecyl sulfate solution, a bromophenol blue solution, a glycerol solution and water.
8. The lead filtering method of a ceramic material manufacturing apparatus as claimed in claim 1, wherein: the water for injection in the third step can be prepared by adopting sterilized normal saline or 5% glucose solution.
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| CN202010320749.XA CN111450052A (en) | 2020-04-22 | 2020-04-22 | Preparation method of urokinase injection |
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| CN202010320749.XA CN111450052A (en) | 2020-04-22 | 2020-04-22 | Preparation method of urokinase injection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114191385A (en) * | 2021-12-22 | 2022-03-18 | 天士力生物医药股份有限公司 | Recombinant human prourokinase injection and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4244943A (en) * | 1978-05-12 | 1981-01-13 | Sumitomo Chemical Company, Limited | Method for preparing urokinase injection |
| CN110894495A (en) * | 2019-12-24 | 2020-03-20 | 江苏尤里卡生物科技有限公司 | Preparation method of urokinase and freeze-dried powder thereof |
-
2020
- 2020-04-22 CN CN202010320749.XA patent/CN111450052A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4244943A (en) * | 1978-05-12 | 1981-01-13 | Sumitomo Chemical Company, Limited | Method for preparing urokinase injection |
| CN110894495A (en) * | 2019-12-24 | 2020-03-20 | 江苏尤里卡生物科技有限公司 | Preparation method of urokinase and freeze-dried powder thereof |
Non-Patent Citations (2)
| Title |
|---|
| 吴梧桐: "《生物制药工艺学》", 28 February 2006, 中国医药科技出版社 * |
| 柳文媛: "《药物分析进展》", 30 September 2018, 江苏凤凰科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114191385A (en) * | 2021-12-22 | 2022-03-18 | 天士力生物医药股份有限公司 | Recombinant human prourokinase injection and preparation method thereof |
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Application publication date: 20200728 |